Bhi01G001222 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G001222
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Fe2OG dioxygenase domain-containing protein
Location	chr1: 34060626 .. 34061529 (+)
RNA-Seq Expression	Bhi01G001222
Synteny	Bhi01G001222

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAGTGGCTGATTCTACCTAGTACATGTGTCCCCGATAGCTGCATCGTAAACATATACGATGAAGGATATTGCATTCCGCCACACATTGATCAACATGACTTTCTAAGACCTTTTTGCACTGTATCATTTCTAACAGAAAGCAACATCATATTTGGCACTCCGACGGAAATTCTTAGTCCTGGAGAGTTCTCCGGTCCTGTTTCGGTTCCTTTGCCTAAAGGGTATGCACACACTATTATTATGCTTTCTTTGTTTCTACATTTCATTTGCTTGGTTATAAATCTAATGTTTGTGTTTCTGTTGATATTGCAGTTCTGTATTGATTTTAAATGGTAATGGAGCTGATCTTGCCAAGCATTGTGTACCTAGTGTCAAATCTAAAAGGTTAGCTATCTGCTATTGTTATTTTACAATCTTCAAATCATTGCCAAAAGAAAAAGGTTCCTTGTGCATCAAATATCTATAAGTTGGTTCCTAATATTTGAATCTGTGGACCTATTACAGGATCTCGATCACATTTCGAAAAATGGAAGACAGGAAACTTCCATACAGGTTTTTGCCTGATCTTGAACTTCAAAGAATCAAGCCACTGGACTTATCTGAATGGCAAGAGGAAGAGAAGAATGTTGAACTGGAGAAAGGTGAAATTGAACAAAGTGATCATTCACCCAAGCCACTGAAATCATTATCCCAATGGCAAGAGGAGAGAAAGAAGAATGTTGAAGGGAAAAAACAGAGGGGGCATCACAATTATGATTCAGTGGACTTCTGTCAGTTGCAGAAGGAAGAGAAGAATGAAAGGAAACAGAGAGACCATCATCATTATCGATATCATTATTCACCCAACCCATCCTCCCAACAAACCAAACGTCAGAGATTCCAAGTTGGTGTCTATTGA

mRNA sequence

ATGGTGAAGTGGCTGATTCTACCTAGTACATGTGTCCCCGATAGCTGCATCGTAAACATATACGATGAAGGATATTGCATTCCGCCACACATTGATCAACATGACTTTCTAAGACCTTTTTGCACTGTATCATTTCTAACAGAAAGCAACATCATATTTGGCACTCCGACGGAAATTCTTAGTCCTGGAGAGTTCTCCGGTCCTGTTTCGGTTCCTTTGCCTAAAGGTTCTGTATTGATTTTAAATGGTAATGGAGCTGATCTTGCCAAGCATTGTGTACCTAGTGTCAAATCTAAAAGGATCTCGATCACATTTCGAAAAATGGAAGACAGGAAACTTCCATACAGGTTTTTGCCTGATCTTGAACTTCAAAGAATCAAGCCACTGGACTTATCTGAATGGCAAGAGGAAGAGAAGAATGTTGAACTGGAGAAAGGTGAAATTGAACAAAGTGATCATTCACCCAAGCCACTGAAATCATTATCCCAATGGCAAGAGGAGAGAAAGAAGAATGTTGAAGGGAAAAAACAGAGGGGGCATCACAATTATGATTCAGTGGACTTCTGTCAGTTGCAGAAGGAAGAGAAGAATGAAAGGAAACAGAGAGACCATCATCATTATCGATATCATTATTCACCCAACCCATCCTCCCAACAAACCAAACGTCAGAGATTCCAAGTTGGTGTCTATTGA

Coding sequence (CDS)

ATGGTGAAGTGGCTGATTCTACCTAGTACATGTGTCCCCGATAGCTGCATCGTAAACATATACGATGAAGGATATTGCATTCCGCCACACATTGATCAACATGACTTTCTAAGACCTTTTTGCACTGTATCATTTCTAACAGAAAGCAACATCATATTTGGCACTCCGACGGAAATTCTTAGTCCTGGAGAGTTCTCCGGTCCTGTTTCGGTTCCTTTGCCTAAAGGTTCTGTATTGATTTTAAATGGTAATGGAGCTGATCTTGCCAAGCATTGTGTACCTAGTGTCAAATCTAAAAGGATCTCGATCACATTTCGAAAAATGGAAGACAGGAAACTTCCATACAGGTTTTTGCCTGATCTTGAACTTCAAAGAATCAAGCCACTGGACTTATCTGAATGGCAAGAGGAAGAGAAGAATGTTGAACTGGAGAAAGGTGAAATTGAACAAAGTGATCATTCACCCAAGCCACTGAAATCATTATCCCAATGGCAAGAGGAGAGAAAGAAGAATGTTGAAGGGAAAAAACAGAGGGGGCATCACAATTATGATTCAGTGGACTTCTGTCAGTTGCAGAAGGAAGAGAAGAATGAAAGGAAACAGAGAGACCATCATCATTATCGATATCATTATTCACCCAACCCATCCTCCCAACAAACCAAACGTCAGAGATTCCAAGTTGGTGTCTATTGA

Protein sequence

MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEILSPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPDLELQRIKPLDLSEWQEEEKNVELEKGEIEQSDHSPKPLKSLSQWQEERKKNVEGKKQRGHHNYDSVDFCQLQKEEKNERKQRDHHHYRYHYSPNPSSQQTKRQRFQVGVY

Homology

[Close]

BLAST of Bhi01G001222 vs. TAIR 10
Match: AT2G17970.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 199.1 bits (505), Expect = 3.8e-51
Identity = 89/131 (67.94%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           ++KW +LP TCVPDSCIVNIYDEG CIPPHID HDFLRPFCT+SFL+E +I+FG+  ++ 
Sbjct: 306 LIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILFGSNLKVE 365

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
            PG+FSG  S+PLP GSVL+LNGNGAD+AKHCVP+V +KRISITFRKM++ K P  F P+
Sbjct: 366 GPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKRPVWFTPE 425

Query: 121 LELQRIKPLDL 132
            +LQ I+PL L
Sbjct: 426 PDLQGIEPLPL 436

[Close]

BLAST of Bhi01G001222 vs. TAIR 10
Match: AT2G17970.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 199.1 bits (505), Expect = 3.8e-51
Identity = 89/131 (67.94%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           ++KW +LP TCVPDSCIVNIYDEG CIPPHID HDFLRPFCT+SFL+E +I+FG+  ++ 
Sbjct: 232 LIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILFGSNLKVE 291

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
            PG+FSG  S+PLP GSVL+LNGNGAD+AKHCVP+V +KRISITFRKM++ K P  F P+
Sbjct: 292 GPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKRPVWFTPE 351

Query: 121 LELQRIKPLDL 132
            +LQ I+PL L
Sbjct: 352 PDLQGIEPLPL 362

[Close]

BLAST of Bhi01G001222 vs. TAIR 10
Match: AT2G17970.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 199.1 bits (505), Expect = 3.8e-51
Identity = 89/131 (67.94%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           ++KW +LP TCVPDSCIVNIYDEG CIPPHID HDFLRPFCT+SFL+E +I+FG+  ++ 
Sbjct: 232 LIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILFGSNLKVE 291

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
            PG+FSG  S+PLP GSVL+LNGNGAD+AKHCVP+V +KRISITFRKM++ K P  F P+
Sbjct: 292 GPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKRPVWFTPE 351

Query: 121 LELQRIKPLDL 132
            +LQ I+PL L
Sbjct: 352 PDLQGIEPLPL 362

[Close]

BLAST of Bhi01G001222 vs. TAIR 10
Match: AT4G36090.2 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-46
Identity = 102/220 (46.36%), Postives = 134/220 (60.91%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           +V W +LP TCVPDSCIVNIY+E  CIPPHID HDFLRPFCTVSFL+E NI+FG+  ++L
Sbjct: 242 LVGWHVLPPTCVPDSCIVNIYEEDDCIPPHIDNHDFLRPFCTVSFLSECNILFGSNLKVL 301

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
            PGEFSG  S+PLP GSVL+L GNGAD+AKHCVP+V +KRISITFRKM++ K P  F P+
Sbjct: 302 GPGEFSGSYSIPLPVGSVLVLKGNGADVAKHCVPAVPTKRISITFRKMDESKRPVGFTPE 361

Query: 121 LELQRIKPLDLSEWQEE---EKNVELEKGEIEQSDHSPKPLKSLSQWQEERKKNVEGKKQ 180
            +L+ IKPL           +  +   +  I+Q+  +             R  +  G K 
Sbjct: 362 PDLEEIKPLPYEHTTPSTPADAVISSSRSSIDQNGSN----------HNNRTAHGGGSKY 421

Query: 181 RGHHNYDSVDFCQLQKEEKNERKQRDHHHYRYHYSPNPSS 218
           R   +Y S      ++    +R++   H     Y P  +S
Sbjct: 422 RRSRDYHSE---SRERSSSGQRRETSRHTPNRSYRPKVTS 448

[Close]

BLAST of Bhi01G001222 vs. TAIR 10
Match: AT4G36090.3 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-46
Identity = 102/220 (46.36%), Postives = 134/220 (60.91%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           +V W +LP TCVPDSCIVNIY+E  CIPPHID HDFLRPFCTVSFL+E NI+FG+  ++L
Sbjct: 310 LVGWHVLPPTCVPDSCIVNIYEEDDCIPPHIDNHDFLRPFCTVSFLSECNILFGSNLKVL 369

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
            PGEFSG  S+PLP GSVL+L GNGAD+AKHCVP+V +KRISITFRKM++ K P  F P+
Sbjct: 370 GPGEFSGSYSIPLPVGSVLVLKGNGADVAKHCVPAVPTKRISITFRKMDESKRPVGFTPE 429

Query: 121 LELQRIKPLDLSEWQEE---EKNVELEKGEIEQSDHSPKPLKSLSQWQEERKKNVEGKKQ 180
            +L+ IKPL           +  +   +  I+Q+  +             R  +  G K 
Sbjct: 430 PDLEEIKPLPYEHTTPSTPADAVISSSRSSIDQNGSN----------HNNRTAHGGGSKY 489

Query: 181 RGHHNYDSVDFCQLQKEEKNERKQRDHHHYRYHYSPNPSS 218
           R   +Y S      ++    +R++   H     Y P  +S
Sbjct: 490 RRSRDYHSE---SRERSSSGQRRETSRHTPNRSYRPKVTS 516

[Close]

BLAST of Bhi01G001222 vs. ExPASy Swiss-Prot
Match: Q9SL49 (RNA demethylase ALKBH9B OS=Arabidopsis thaliana OX=3702 GN=ALKBH9B PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 5.3e-50
Identity = 89/131 (67.94%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           ++KW +LP TCVPDSCIVNIYDEG CIPPHID HDFLRPFCT+SFL+E +I+FG+  ++ 
Sbjct: 306 LIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILFGSNLKVE 365

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
            PG+FSG  S+PLP GSVL+LNGNGAD+AKHCVP+V +KRISITFRKM++ K P  F P+
Sbjct: 366 GPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKRPVWFTPE 425

Query: 121 LELQRIKPLDL 132
            +LQ I+PL L
Sbjct: 426 PDLQGIEPLPL 436

[Close]

BLAST of Bhi01G001222 vs. ExPASy Swiss-Prot
Match: Q9ZT92 (RNA demethylase ALKBH10B OS=Arabidopsis thaliana OX=3702 GN=ALKBH10B PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.0e-13
Identity = 38/109 (34.86%), Postives = 66/109 (60.55%), Query Frame = 0


Query: 2   VKWLILPSTCVPDSCIVNIYDEG-----YCIPPHIDQHDFLRPFCTVSFLTESNIIFGTP 61
           V W ++P    P+ C++N ++EG     +  PPH++Q     P  T+  L+ES + +G  
Sbjct: 333 VTWRLIPEYKRPNGCVINFFEEGEYSQPFLKPPHLEQ-----PISTL-VLSESTMAYGRI 392

Query: 62  TEILSPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITF 106
               + G F GP+++ L +GS+L++ GN AD+A+H +   ++KR+SITF
Sbjct: 393 LSSDNEGNFRGPLTLSLKQGSLLVMRGNSADMARHVMCPSQNKRVSITF 435

[Close]

BLAST of Bhi01G001222 vs. ExPASy Swiss-Prot
Match: E1BH29 (RNA demethylase ALKBH5 OS=Bos taurus OX=9913 GN=ALKBH5 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 9.2e-10
Identity = 41/111 (36.94%), Postives = 62/111 (55.86%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQ-HDFLRPFCTVSFLTESNIIFGTPTEI 60
           +V+  ++P   V +S ++N Y  G CI  H+D  H F RP  +VSF ++S + FG   + 
Sbjct: 176 LVEHRVIPEGFV-NSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQ- 235

Query: 61  LSPGEFSGPV-SVPLPKGSVLILNGNGADLAKHCV--PSVKSKRISITFRK 108
             P   S PV S+P+ +GSV +L+G  AD   HC+    +K +R  I  RK
Sbjct: 236 FKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRK 284

[Close]

BLAST of Bhi01G001222 vs. ExPASy Swiss-Prot
Match: Q6P6C2 (RNA demethylase ALKBH5 OS=Homo sapiens OX=9606 GN=ALKBH5 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 9.2e-10
Identity = 41/111 (36.94%), Postives = 62/111 (55.86%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQ-HDFLRPFCTVSFLTESNIIFGTPTEI 60
           +V+  ++P   V +S ++N Y  G CI  H+D  H F RP  +VSF ++S + FG   + 
Sbjct: 176 LVEHRVIPEGFV-NSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQ- 235

Query: 61  LSPGEFSGPV-SVPLPKGSVLILNGNGADLAKHCV--PSVKSKRISITFRK 108
             P   S PV S+P+ +GSV +L+G  AD   HC+    +K +R  I  RK
Sbjct: 236 FKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRK 284

[Close]

BLAST of Bhi01G001222 vs. ExPASy Swiss-Prot
Match: Q3TSG4 (RNA demethylase ALKBH5 OS=Mus musculus OX=10090 GN=Alkbh5 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 9.2e-10
Identity = 41/111 (36.94%), Postives = 62/111 (55.86%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQ-HDFLRPFCTVSFLTESNIIFGTPTEI 60
           +V+  ++P   V +S ++N Y  G CI  H+D  H F RP  +VSF ++S + FG   + 
Sbjct: 177 LVEHRVIPEGFV-NSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQ- 236

Query: 61  LSPGEFSGPV-SVPLPKGSVLILNGNGADLAKHCV--PSVKSKRISITFRK 108
             P   S PV S+P+ +GSV +L+G  AD   HC+    +K +R  I  RK
Sbjct: 237 FKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRK 285

[Close]

BLAST of Bhi01G001222 vs. NCBI nr
Match: XP_022149186.1 (RNA demethylase ALKBH5-like [Momordica charantia])

HSP 1 Score: 308.1 bits (788), Expect = 6.2e-80
Identity = 172/250 (68.80%), Postives = 199/250 (79.60%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MVKW ILP TCVPDSCIVNIYDEG CIPPHID HDFLRPFCTVSFLTESNI+FG+  EIL
Sbjct: 315 MVKWHILPRTCVPDSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTESNILFGSRLEIL 374

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GS L+L+GNGADLAKHCVPSV +KRISITFRKM+DRKLPYRFLPD
Sbjct: 375 SPGEFSGPVSIPLPMGSALVLSGNGADLAKHCVPSVSAKRISITFRKMDDRKLPYRFLPD 434

Query: 121 LELQRIKPLDLSEW---QEEEKNVELEKGEIEQSD----HSPKPLKSLSQWQEERKKNV- 180
           LELQRIKPL+LS++    EEE+ V+ E GE +Q D    HSP PL  L+QWQEE +KNV 
Sbjct: 435 LELQRIKPLNLSQFLEEGEEEEIVQAEDGERKQRDHHHFHSPMPL-DLTQWQEEERKNVR 494

Query: 181 --EGK-KQRGHHNYDS---VDFCQLQKEEKN------ERKQRDHHHYRYHYSPNPSSQQT 231
             EGK KQR H++Y S   +D  + QKEEKN      E KQR HH+Y   Y+P  SSQ++
Sbjct: 495 VKEGKRKQRDHYHYYSPKPLDLTRWQKEEKNVQLEEGEIKQRIHHNY---YTPKRSSQRS 554

[Close]

BLAST of Bhi01G001222 vs. NCBI nr
Match: XP_039002799.1 (RNA demethylase ALKBH9B-like [Hibiscus syriacus] >KAE8702262.1 phosphatidylinositol-4-phosphate 5-kinase 8-like [Hibiscus syriacus])

HSP 1 Score: 220.7 bits (561), Expect = 1.3e-53
Identity = 102/132 (77.27%), Postives = 116/132 (87.88%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MV+W ILP TC+P+SCIVNIYDEG CIPPHID HDFLRPFCTVSFLT+ NI+FG+  +I 
Sbjct: 211 MVRWHILPPTCIPNSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTQCNILFGSSLKIA 270

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GSVLILNGNGAD+AKHCVP+V  KRISITFR+M+D KLPY+F PD
Sbjct: 271 SPGEFSGPVSIPLPVGSVLILNGNGADIAKHCVPAVPGKRISITFRRMDDSKLPYQFSPD 330

Query: 121 LELQRIKPLDLS 133
            EL RIKPL +S
Sbjct: 331 PELLRIKPLVVS 342

[Close]

BLAST of Bhi01G001222 vs. NCBI nr
Match: XP_021617215.1 (uncharacterized protein LOC110618397 isoform X2 [Manihot esculenta] >KAG8650240.1 hypothetical protein MANES_07G016100v8 [Manihot esculenta])

HSP 1 Score: 220.3 bits (560), Expect = 1.7e-53
Identity = 113/183 (61.75%), Postives = 132/183 (72.13%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MV+W +LP+TC+P+SCIVNIYDEG CIPPHID HDFLRPFCTVSFLTE NI+FG+  +IL
Sbjct: 259 MVRWHVLPATCIPNSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTECNIVFGSNLKIL 318

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GSVL+LNGNGAD+AKHC+P V +KRISITFRKM+D KLPY+F PD
Sbjct: 319 SPGEFSGPVSIPLPVGSVLVLNGNGADIAKHCIPGVPAKRISITFRKMDDSKLPYQFSPD 378

Query: 121 LELQRIKPLDLSEWQEEEKNVELEKGEIEQSDHS-----PKPLKSLSQWQEERKKNVEGK 179
            EL  IKPL  S          L K   +QS        P  +KSL Q    +  N+   
Sbjct: 379 PELLGIKPLIYS---------PLTKSPYQQSQQQKPFGYPSVVKSLVQQSNHQNGNLTAT 432

[Close]

BLAST of Bhi01G001222 vs. NCBI nr
Match: XP_021617214.1 (uncharacterized protein LOC110618397 isoform X1 [Manihot esculenta] >OAY44915.1 hypothetical protein MANES_07G016100v8 [Manihot esculenta])

HSP 1 Score: 220.3 bits (560), Expect = 1.7e-53
Identity = 113/183 (61.75%), Postives = 132/183 (72.13%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MV+W +LP+TC+P+SCIVNIYDEG CIPPHID HDFLRPFCTVSFLTE NI+FG+  +IL
Sbjct: 284 MVRWHVLPATCIPNSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTECNIVFGSNLKIL 343

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GSVL+LNGNGAD+AKHC+P V +KRISITFRKM+D KLPY+F PD
Sbjct: 344 SPGEFSGPVSIPLPVGSVLVLNGNGADIAKHCIPGVPAKRISITFRKMDDSKLPYQFSPD 403

Query: 121 LELQRIKPLDLSEWQEEEKNVELEKGEIEQSDHS-----PKPLKSLSQWQEERKKNVEGK 179
            EL  IKPL  S          L K   +QS        P  +KSL Q    +  N+   
Sbjct: 404 PELLGIKPLIYS---------PLTKSPYQQSQQQKPFGYPSVVKSLVQQSNHQNGNLTAT 457

[Close]

BLAST of Bhi01G001222 vs. NCBI nr
Match: EOX97320.1 (Oxidoreductase, putative [Theobroma cacao])

HSP 1 Score: 218.8 bits (556), Expect = 4.9e-53
Identity = 103/132 (78.03%), Postives = 114/132 (86.36%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MV+W ILP TCVP+SCIVNIYDEG CIPPHID HDFLRPFCTVSFLTESNI+FG+  +I+
Sbjct: 252 MVRWHILPPTCVPNSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTESNILFGSSLKIV 311

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GSVLILNGNGAD+AKHCVP V  KRISITFR+M+D KLPY+F  D
Sbjct: 312 SPGEFSGPVSIPLPVGSVLILNGNGADIAKHCVPGVPGKRISITFRRMDDSKLPYKFSSD 371

Query: 121 LELQRIKPLDLS 133
            EL  IKPL  S
Sbjct: 372 PELLGIKPLIFS 383

[Close]

BLAST of Bhi01G001222 vs. ExPASy TrEMBL
Match: A0A6J1D7M0 (RNA demethylase ALKBH5-like OS=Momordica charantia OX=3673 GN=LOC111017672 PE=4 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 3.0e-80
Identity = 172/250 (68.80%), Postives = 199/250 (79.60%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MVKW ILP TCVPDSCIVNIYDEG CIPPHID HDFLRPFCTVSFLTESNI+FG+  EIL
Sbjct: 315 MVKWHILPRTCVPDSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTESNILFGSRLEIL 374

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GS L+L+GNGADLAKHCVPSV +KRISITFRKM+DRKLPYRFLPD
Sbjct: 375 SPGEFSGPVSIPLPMGSALVLSGNGADLAKHCVPSVSAKRISITFRKMDDRKLPYRFLPD 434

Query: 121 LELQRIKPLDLSEW---QEEEKNVELEKGEIEQSD----HSPKPLKSLSQWQEERKKNV- 180
           LELQRIKPL+LS++    EEE+ V+ E GE +Q D    HSP PL  L+QWQEE +KNV 
Sbjct: 435 LELQRIKPLNLSQFLEEGEEEEIVQAEDGERKQRDHHHFHSPMPL-DLTQWQEEERKNVR 494

Query: 181 --EGK-KQRGHHNYDS---VDFCQLQKEEKN------ERKQRDHHHYRYHYSPNPSSQQT 231
             EGK KQR H++Y S   +D  + QKEEKN      E KQR HH+Y   Y+P  SSQ++
Sbjct: 495 VKEGKRKQRDHYHYYSPKPLDLTRWQKEEKNVQLEEGEIKQRIHHNY---YTPKRSSQRS 554

[Close]

BLAST of Bhi01G001222 vs. ExPASy TrEMBL
Match: A0A6A3AHL3 (Phosphatidylinositol-4-phosphate 5-kinase 8-like OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110485pilonHSYRG00154 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 6.3e-54
Identity = 102/132 (77.27%), Postives = 116/132 (87.88%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MV+W ILP TC+P+SCIVNIYDEG CIPPHID HDFLRPFCTVSFLT+ NI+FG+  +I 
Sbjct: 211 MVRWHILPPTCIPNSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTQCNILFGSSLKIA 270

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GSVLILNGNGAD+AKHCVP+V  KRISITFR+M+D KLPY+F PD
Sbjct: 271 SPGEFSGPVSIPLPVGSVLILNGNGADIAKHCVPAVPGKRISITFRRMDDSKLPYQFSPD 330

Query: 121 LELQRIKPLDLS 133
            EL RIKPL +S
Sbjct: 331 PELLRIKPLVVS 342

[Close]

BLAST of Bhi01G001222 vs. ExPASy TrEMBL
Match: A0A2C9VHM6 (Fe2OG dioxygenase domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_07G016100 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 8.2e-54
Identity = 113/183 (61.75%), Postives = 132/183 (72.13%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MV+W +LP+TC+P+SCIVNIYDEG CIPPHID HDFLRPFCTVSFLTE NI+FG+  +IL
Sbjct: 284 MVRWHVLPATCIPNSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTECNIVFGSNLKIL 343

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GSVL+LNGNGAD+AKHC+P V +KRISITFRKM+D KLPY+F PD
Sbjct: 344 SPGEFSGPVSIPLPVGSVLVLNGNGADIAKHCIPGVPAKRISITFRKMDDSKLPYQFSPD 403

Query: 121 LELQRIKPLDLSEWQEEEKNVELEKGEIEQSDHS-----PKPLKSLSQWQEERKKNVEGK 179
            EL  IKPL  S          L K   +QS        P  +KSL Q    +  N+   
Sbjct: 404 PELLGIKPLIYS---------PLTKSPYQQSQQQKPFGYPSVVKSLVQQSNHQNGNLTAT 457

[Close]

BLAST of Bhi01G001222 vs. ExPASy TrEMBL
Match: A0A061DXA1 (Oxidoreductase, putative OS=Theobroma cacao OX=3641 GN=TCM_006385 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 2.4e-53
Identity = 103/132 (78.03%), Postives = 114/132 (86.36%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MV+W ILP TCVP+SCIVNIYDEG CIPPHID HDFLRPFCTVSFLTESNI+FG+  +I+
Sbjct: 252 MVRWHILPPTCVPNSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTESNILFGSSLKIV 311

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GSVLILNGNGAD+AKHCVP V  KRISITFR+M+D KLPY+F  D
Sbjct: 312 SPGEFSGPVSIPLPVGSVLILNGNGADIAKHCVPGVPGKRISITFRRMDDSKLPYKFSSD 371

Query: 121 LELQRIKPLDLS 133
            EL  IKPL  S
Sbjct: 372 PELLGIKPLIFS 383

[Close]

BLAST of Bhi01G001222 vs. ExPASy TrEMBL
Match: A0A2P5WEQ1 (Fe2OG dioxygenase domain-containing protein OS=Gossypium barbadense OX=3634 GN=ES319_A04G155400v1 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 4.1e-53
Identity = 106/160 (66.25%), Postives = 126/160 (78.75%), Query Frame = 0


Query: 1   MVKWLILPSTCVPDSCIVNIYDEGYCIPPHIDQHDFLRPFCTVSFLTESNIIFGTPTEIL 60
           MV+W +LP+ CVP+SCIVNIYDEG CIPPHID HDFLRPFCTVSFLT+ NI+FG+  +I+
Sbjct: 240 MVRWHVLPTMCVPNSCIVNIYDEGDCIPPHIDHHDFLRPFCTVSFLTQCNILFGSSLKIV 299

Query: 61  SPGEFSGPVSVPLPKGSVLILNGNGADLAKHCVPSVKSKRISITFRKMEDRKLPYRFLPD 120
           SPGEFSGPVS+PLP GSVLILNGNGAD+AKHCVP+V  KRISITFR+M++ KLPY+FLPD
Sbjct: 300 SPGEFSGPVSIPLPVGSVLILNGNGADIAKHCVPAVPGKRISITFRRMDESKLPYKFLPD 359

Query: 121 LELQRIKPLDLS-------EWQEEEKNVELEKGEIEQSDH 154
            EL RIKP  +S         Q+   N    K  I+Q+ H
Sbjct: 360 PELSRIKPFIVSPSSAPQGHHQKPVINSSFVKAIIQQNQH 399

The following BLAST results are available for this feature:

BLAST of Bhi01G001222 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G17970.1	3.8e-51	67.94	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17970.2	3.8e-51	67.94	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17970.3	3.8e-51	67.94	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G36090.2	1.3e-46	46.36	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]
AT4G36090.3	1.3e-46	46.36	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]

BLAST of Bhi01G001222 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SL49	5.3e-50	67.94	RNA demethylase ALKBH9B OS=Arabidopsis thaliana OX=3702 GN=ALKBH9B PE=1 SV=1	[more]
Q9ZT92	3.0e-13	34.86	RNA demethylase ALKBH10B OS=Arabidopsis thaliana OX=3702 GN=ALKBH10B PE=1 SV=1	[more]
E1BH29	9.2e-10	36.94	RNA demethylase ALKBH5 OS=Bos taurus OX=9913 GN=ALKBH5 PE=3 SV=1	[more]
Q6P6C2	9.2e-10	36.94	RNA demethylase ALKBH5 OS=Homo sapiens OX=9606 GN=ALKBH5 PE=1 SV=2	[more]
Q3TSG4	9.2e-10	36.94	RNA demethylase ALKBH5 OS=Mus musculus OX=10090 GN=Alkbh5 PE=1 SV=2	[more]

BLAST of Bhi01G001222 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149186.1	6.2e-80	68.80	RNA demethylase ALKBH5-like [Momordica charantia]	[more]
XP_039002799.1	1.3e-53	77.27	RNA demethylase ALKBH9B-like [Hibiscus syriacus] >KAE8702262.1 phosphatidylinosi...	[more]
XP_021617215.1	1.7e-53	61.75	uncharacterized protein LOC110618397 isoform X2 [Manihot esculenta] >KAG8650240....	[more]
XP_021617214.1	1.7e-53	61.75	uncharacterized protein LOC110618397 isoform X1 [Manihot esculenta] >OAY44915.1 ...	[more]
EOX97320.1	4.9e-53	78.03	Oxidoreductase, putative [Theobroma cacao]	[more]

BLAST of Bhi01G001222 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7M0	3.0e-80	68.80	RNA demethylase ALKBH5-like OS=Momordica charantia OX=3673 GN=LOC111017672 PE=4 ...	[more]
A0A6A3AHL3	6.3e-54	77.27	Phosphatidylinositol-4-phosphate 5-kinase 8-like OS=Hibiscus syriacus OX=106335 ...	[more]
A0A2C9VHM6	8.2e-54	61.75	Fe2OG dioxygenase domain-containing protein OS=Manihot esculenta OX=3983 GN=MANE...	[more]
A0A061DXA1	2.4e-53	78.03	Oxidoreductase, putative OS=Theobroma cacao OX=3641 GN=TCM_006385 PE=4 SV=1	[more]
A0A2P5WEQ1	4.1e-53	66.25	Fe2OG dioxygenase domain-containing protein OS=Gossypium barbadense OX=3634 GN=E...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 131..151
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 162..202
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 142..230
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 211..230
None	No IPR available	PANTHER	PTHR31447:SF1	2OG-FE(II) OXYGENASE	coord: 1..203
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 9..108
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 1..113 e-value: 2.5E-38 score: 133.8
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 8..106 e-value: 5.3E-10 score: 39.8
IPR044842	RNA demethylase ALKBH9B/ALKBH10B-like	PANTHER	PTHR31447	HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN-RELATED	coord: 1..203
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 12..109 score: 9.538495

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M001222	Bhi01M001222	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070988	demethylation
biological_process	GO:0032259	methylation
biological_process	GO:0006402	mRNA catabolic process
molecular_function	GO:0032451	demethylase activity
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003729	mRNA binding