Bhi01G001178 (gene) Wax gourd (B227) v1

Overview
NameBhi01G001178
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionhyccin
Locationchr1: 32259690 .. 32261503 (+)
RNA-Seq ExpressionBhi01G001178
SyntenyBhi01G001178
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCTATCATTCATCTAGAATCAGAGTTTTTAAAATTATGTAATTAAGTTGTCTTTTTAAACAATTGAATCAAACGTATAACTTAATTAAAAATGAAATAAATGAAATAAAATAATAAAAATTCATGGTTACATGTAGTTGCCTAAGTAGTGGATGAAAGTTTCGAGAGATGGAGAATTTGAAGGGAAAGGTCTCAACGTTGTAAGCTCGATCCTATTTCTCCTCGTTTCTTTTCCCTCCACACTTTCCTTTCATTTTACCTTCAAACTTTCAAACCCCGTTTTCCCAAGAAAAAACAATAAAAAGAAAATAAGAAAAACAAAACTCAAATATCAAATTCCATTATATTTTATTTTATTTTATTTTATTTATTTATTTTAAAGAAAACTAATCAATTTCCACCAATGTCCTCCTCTTCCTCCGACGACGACTCCCCCGCCGCCGTAGAACCAACCCCCGCAGAAGAACCCGCCGCCGAAGAAAAACCAACCCCCGCAGAAGAAACTGCCATCGATGAAACCGCCGCCGCCGAAGAAACCACCCCCACCATCGCCGCCGCCGAAGCACCCGTCATCAAAACCAGCAGCAGAGCAAGCGGAAGCGGCCCCGTGGTGAGATTCGATATCTCACAATCGTCATCCTTAACCACAATCGCAAAAACCGCCATTGAATCCCTAAAATCCATTCTCCCAAATATCCCCTCATCTCTCCCTTCCGCACCCAACCCCGCCCTCGCCCTCCTCAACGACCTCGAAACCACCGCCCAAATCACCGCCCTCCTCCGCCGCCCCACCTCCGGCGCCGGCGACGACAACCTCTGCCGTTGGCTCTACGATACTTTCCAATCCAACAACCCCGACCTCAAACTCGTCGTCCTCCGCTTCCTCCCTGTTCTCCTCGGCGCTTACCTCTCCCGTGTCGTCTCCCGCCGCAAATCCCTCGCCGGGTTCGAAGCCGTTCTCCTCTCCCTCTACGCCCATGAAACCAACCGCCGCGCCAGCCAACCCCTCTCCGTCAACATCCCCGATTTAACCCACCCCAGTATTTACCACGAATCCAAATCCCCTCTCAAAAACAATGCCACCGCTCTCAATCTCGCCGTAATCTCCCCATCGTTGGAGCCCCACGGTATGGTCCGCTCCACCAAGAGGGCTCGAATCGTCGGCGTCGCCCTCGAACTCTACTACACGAAAATCGATAAGATTCCAGAAACTTCCAAGATTGAGTTCTGCGAGTTTTGCCGGATCTGGGCCAGCGGCGGCGATGATGAAAAGGGAAAAGTTAAAAATGAGGAAGCGGCGGCGGAGGAGGCAGAGGAGGAGAGTATTGGGAGAATTCCGTTGCCGTGGGAGATGCTTCAGCCGATTTTAAGAGTGTTGGGGCACTGTCTTTTGGGGTCGAATGTGACTGCGAAGTGCAAGAAGAATGAAACGACCCCGTTGTTCGATGCAGCCATTGCTGCGATTCGGAGCTTGTATTTGAGATCGATGCATGACATTAATCCCAAAGCAATTTTGGCGACTGGGAGTTTGGTGAGGTTGGGGAATATGGCTATGGAGTCGGCTGATGAAATTGATTACACTGAGATTCCTTATCAAACTGTCATCAACCTCTAGCAATTCATTTTTTCTTCTTTTTCTCTTCAGATCAAATGAATGGTTGCAATCTTTTTTCTTGCCTTTTTCTCTTTTTTGTTTGGATTCTTTAATTTTCTTTAGTGAATTTGGAGTTGTAAAATAAAATGTACTTTATAGAGAATTGTCTTTGTTGGCTTATTTTTGTTTGGTTGATATATTTGTAGTCCCCTTTACCTTC

mRNA sequence

TCCTATCATTCATCTAGAATCAGAGTTTTTAAAATTATGTAATTAAGTTGTCTTTTTAAACAATTGAATCAAACGTATAACTTAATTAAAAATGAAATAAATGAAATAAAATAATAAAAATTCATGGTTACATGTAGTTGCCTAAGTAGTGGATGAAAGTTTCGAGAGATGGAGAATTTGAAGGGAAAGGTCTCAACGTTGTAAGCTCGATCCTATTTCTCCTCGTTTCTTTTCCCTCCACACTTTCCTTTCATTTTACCTTCAAACTTTCAAACCCCGTTTTCCCAAGAAAAAACAATAAAAAGAAAATAAGAAAAACAAAACTCAAATATCAAATTCCATTATATTTTATTTTATTTTATTTTATTTATTTATTTTAAAGAAAACTAATCAATTTCCACCAATGTCCTCCTCTTCCTCCGACGACGACTCCCCCGCCGCCGTAGAACCAACCCCCGCAGAAGAACCCGCCGCCGAAGAAAAACCAACCCCCGCAGAAGAAACTGCCATCGATGAAACCGCCGCCGCCGAAGAAACCACCCCCACCATCGCCGCCGCCGAAGCACCCGTCATCAAAACCAGCAGCAGAGCAAGCGGAAGCGGCCCCGTGGTGAGATTCGATATCTCACAATCGTCATCCTTAACCACAATCGCAAAAACCGCCATTGAATCCCTAAAATCCATTCTCCCAAATATCCCCTCATCTCTCCCTTCCGCACCCAACCCCGCCCTCGCCCTCCTCAACGACCTCGAAACCACCGCCCAAATCACCGCCCTCCTCCGCCGCCCCACCTCCGGCGCCGGCGACGACAACCTCTGCCGTTGGCTCTACGATACTTTCCAATCCAACAACCCCGACCTCAAACTCGTCGTCCTCCGCTTCCTCCCTGTTCTCCTCGGCGCTTACCTCTCCCGTGTCGTCTCCCGCCGCAAATCCCTCGCCGGGTTCGAAGCCGTTCTCCTCTCCCTCTACGCCCATGAAACCAACCGCCGCGCCAGCCAACCCCTCTCCGTCAACATCCCCGATTTAACCCACCCCAGTATTTACCACGAATCCAAATCCCCTCTCAAAAACAATGCCACCGCTCTCAATCTCGCCGTAATCTCCCCATCGTTGGAGCCCCACGGTATGGTCCGCTCCACCAAGAGGGCTCGAATCGTCGGCGTCGCCCTCGAACTCTACTACACGAAAATCGATAAGATTCCAGAAACTTCCAAGATTGAGTTCTGCGAGTTTTGCCGGATCTGGGCCAGCGGCGGCGATGATGAAAAGGGAAAAGTTAAAAATGAGGAAGCGGCGGCGGAGGAGGCAGAGGAGGAGAGTATTGGGAGAATTCCGTTGCCGTGGGAGATGCTTCAGCCGATTTTAAGAGTGTTGGGGCACTGTCTTTTGGGGTCGAATGTGACTGCGAAGTGCAAGAAGAATGAAACGACCCCGTTGTTCGATGCAGCCATTGCTGCGATTCGGAGCTTGTATTTGAGATCGATGCATGACATTAATCCCAAAGCAATTTTGGCGACTGGGAGTTTGGTGAGGTTGGGGAATATGGCTATGGAGTCGGCTGATGAAATTGATTACACTGAGATTCCTTATCAAACTGTCATCAACCTCTAGCAATTCATTTTTTCTTCTTTTTCTCTTCAGATCAAATGAATGGTTGCAATCTTTTTTCTTGCCTTTTTCTCTTTTTTGTTTGGATTCTTTAATTTTCTTTAGTGAATTTGGAGTTGTAAAATAAAATGTACTTTATAGAGAATTGTCTTTGTTGGCTTATTTTTGTTTGGTTGATATATTTGTAGTCCCCTTTACCTTC

Coding sequence (CDS)

ATGTCCTCCTCTTCCTCCGACGACGACTCCCCCGCCGCCGTAGAACCAACCCCCGCAGAAGAACCCGCCGCCGAAGAAAAACCAACCCCCGCAGAAGAAACTGCCATCGATGAAACCGCCGCCGCCGAAGAAACCACCCCCACCATCGCCGCCGCCGAAGCACCCGTCATCAAAACCAGCAGCAGAGCAAGCGGAAGCGGCCCCGTGGTGAGATTCGATATCTCACAATCGTCATCCTTAACCACAATCGCAAAAACCGCCATTGAATCCCTAAAATCCATTCTCCCAAATATCCCCTCATCTCTCCCTTCCGCACCCAACCCCGCCCTCGCCCTCCTCAACGACCTCGAAACCACCGCCCAAATCACCGCCCTCCTCCGCCGCCCCACCTCCGGCGCCGGCGACGACAACCTCTGCCGTTGGCTCTACGATACTTTCCAATCCAACAACCCCGACCTCAAACTCGTCGTCCTCCGCTTCCTCCCTGTTCTCCTCGGCGCTTACCTCTCCCGTGTCGTCTCCCGCCGCAAATCCCTCGCCGGGTTCGAAGCCGTTCTCCTCTCCCTCTACGCCCATGAAACCAACCGCCGCGCCAGCCAACCCCTCTCCGTCAACATCCCCGATTTAACCCACCCCAGTATTTACCACGAATCCAAATCCCCTCTCAAAAACAATGCCACCGCTCTCAATCTCGCCGTAATCTCCCCATCGTTGGAGCCCCACGGTATGGTCCGCTCCACCAAGAGGGCTCGAATCGTCGGCGTCGCCCTCGAACTCTACTACACGAAAATCGATAAGATTCCAGAAACTTCCAAGATTGAGTTCTGCGAGTTTTGCCGGATCTGGGCCAGCGGCGGCGATGATGAAAAGGGAAAAGTTAAAAATGAGGAAGCGGCGGCGGAGGAGGCAGAGGAGGAGAGTATTGGGAGAATTCCGTTGCCGTGGGAGATGCTTCAGCCGATTTTAAGAGTGTTGGGGCACTGTCTTTTGGGGTCGAATGTGACTGCGAAGTGCAAGAAGAATGAAACGACCCCGTTGTTCGATGCAGCCATTGCTGCGATTCGGAGCTTGTATTTGAGATCGATGCATGACATTAATCCCAAAGCAATTTTGGCGACTGGGAGTTTGGTGAGGTTGGGGAATATGGCTATGGAGTCGGCTGATGAAATTGATTACACTGAGATTCCTTATCAAACTGTCATCAACCTCTAG

Protein sequence

MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAAAEETTPTIAAAEAPVIKTSSRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPNIPSSLPSAPNPALALLNDLETTAQITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEEAAAEEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAIRSLYLRSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL
Homology
BLAST of Bhi01G001178 vs. TAIR 10
Match: AT5G21050.1 (LOCATED IN: chloroplast; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Hyccin (InterPro:IPR018619); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G64090.1); Has 206 Blast hits to 206 proteins in 60 species: Archae - 0; Bacteria - 0; Metazoa - 145; Fungi - 0; Plants - 50; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 332.0 bits (850), Expect = 6.5e-91
Identity = 190/364 (52.20%), Postives = 242/364 (66.48%), Query Frame = 0

Query: 59  TSSRASGSGPVVRFD-----ISQSSSLTTIAKTAIESLKSILPNIPSSLPSAPNPALALL 118
           +SS  S   P +  D      +  S   T  +TAI+SL +I+ N      + P+    LL
Sbjct: 5   SSSHDSPPSPAITGDSETTVTNNESESNTKCQTAIQSLSTIVTN-----TNIPSTITILL 64

Query: 119 NDLETTAQITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVV 178
           +D   +  I++LL RP SGAGD+NLCRWLYDTFQS  P L+L+VLRF+P++ G YLSRV 
Sbjct: 65  DDEAVSTAISSLLLRPDSGAGDNNLCRWLYDTFQSAEPSLQLLVLRFVPLIAGLYLSRVP 124

Query: 179 SRRKSLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLK-NNATALNLA 238
            R+   AGFEAVLL+LYAHET  RA Q ++VNIPDL++PSIYHESK   + NN+T LN+A
Sbjct: 125 LRQPQ-AGFEAVLLALYAHETTSRAGQAITVNIPDLSYPSIYHESKGLTRNNNSTCLNIA 184

Query: 239 VISPSLEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA-SGGDDEKG 298
           VIS +L+PHG VRST+RARIVGVALELYY+KI K+P  SK+ FCE C  WA   G+ E+ 
Sbjct: 185 VISSTLDPHGTVRSTRRARIVGVALELYYSKISKMPRESKLNFCESCEKWAGQNGETEQS 244

Query: 299 K------------VKNEEAAAEEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCK 358
                         + E  A     E   GRIPLPWE+LQPILR+LGHCLLG        
Sbjct: 245 SRAVIPTLSDDSWREEENVAIGGRSERDSGRIPLPWELLQPILRILGHCLLG-------L 304

Query: 359 KNETTPLFDAAIAAIRSLYLRSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQT 404
           K E   L +AA  A +SLYLRS+HDINPKAILATGSL+RL  MA++  ++ID+TE+   T
Sbjct: 305 KMEDRELSEAANKACQSLYLRSLHDINPKAILATGSLLRLREMALDPKNQIDHTELSNDT 355

BLAST of Bhi01G001178 vs. TAIR 10
Match: AT5G64090.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Hyccin (InterPro:IPR018619); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G21050.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 219.9 bits (559), Expect = 3.6e-57
Identity = 161/424 (37.97%), Postives = 221/424 (52.12%), Query Frame = 0

Query: 28  PTPAEETAIDETAAAEETTPTIAAAEAPVIKTSSRASGSGPVVRFDISQSSSLTTIAKTA 87
           P+P+  T+       +  T   A A A    + S A+   P+  +  S S       ++ 
Sbjct: 12  PSPSSSTSSSTPHRFKSVTTPTATAAAVSGFSPSAAADRDPMHSWWESVSKQ-----RSR 71

Query: 88  IESLKSILPNIP----------SSLPSAPNPALALLNDLETTAQITALLRRPTSGAGDDN 147
           I SL S+L              SSL  +  PAL+LL+     + I+  L  P SG+G D 
Sbjct: 72  ILSLSSLLSGDSHFEDGDVTPISSLADSDRPALSLLSSRAAYSLISNSLCNPASGSGSDP 131

Query: 148 LCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRK----SLAGFEAVLLSLYAHE 207
           LC+WLY+T+ S++P L+LVVL F P+L+G YLSR+ S       SL+GFEAVLL++YA E
Sbjct: 132 LCQWLYETYLSSDPPLRLVVLSFFPLLVGMYLSRIHSSDSTSLPSLSGFEAVLLAIYAAE 191

Query: 208 TNRRASQPLSVNIPDLTHPSIYHESKSPL----KNNATALNLAVISPSLEPHGMVRSTKR 267
              RA +P+ V+IPDL+ PS+YH  ++ +     +N TA ++ V+SP LEP   V+STKR
Sbjct: 192 VKARAGKPILVHIPDLSQPSLYHTPRNGVDKSRDSNPTA-SVGVLSPQLEPQIAVKSTKR 251

Query: 268 ARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEE------------ 327
           A IVGV L+ Y+ +I ++P  SK+EFC+F   WA    D K K+  +E            
Sbjct: 252 ASIVGVGLQCYFKEISQMPAWSKLEFCKFSASWAGQDCDCKEKIDEDEDKVLALTNGFGD 311

Query: 328 --------------------AAAEEAEEE--------SIG---RIPLPWEMLQPILRVLG 387
                                A  E EE+         +G   RIPLPWE+ QP LR+LG
Sbjct: 312 SSSFNGSSGRSLEIEEDFDRLAIRENEEQLSSNGGGGGVGRGVRIPLPWELFQPTLRILG 371

Query: 388 HCLLGSNVTAKCKKNETTPLFDAAIAAIRSLYLRSMHDINPKAILATGSLVRLGNMAMES 391
           HCLL    T   K        DAA  A+RSLY R+ HD+NP+AILAT SLV L   A  S
Sbjct: 372 HCLLSPLNTEDVK--------DAASNAVRSLYARASHDLNPQAILATRSLVNLDTSARTS 421

BLAST of Bhi01G001178 vs. ExPASy Swiss-Prot
Match: Q9BYI3 (Hyccin OS=Homo sapiens OX=9606 GN=FAM126A PE=1 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 5.2e-08
Identity = 56/208 (26.92%), Postives = 92/208 (44.23%), Query Frame = 0

Query: 82  TIAKTAIESLKSILPNIPSSLPSAPNPALALLNDLETTAQITALLRRPTSGAGDDNLCRW 141
           T  K  +E   S    +P +  S PN A  L +     + +  +++ P S   +  +C  
Sbjct: 3   TSEKGVVEEWLSEFKTLPET--SLPNYATNLKDKSSLVSSLYKVIQEPQSELLEP-VCHQ 62

Query: 142 LYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLAG-FEAVLLSLYAHE--TNRRA 201
           L++ ++S    L    L+FLP L+  YL+   SR    +G  EA+LL +Y  E    +  
Sbjct: 63  LFEFYRSGEEQLLQFTLQFLPELIWCYLAVSASRNVHSSGCIEALLLGVYNLEIVDKQGH 122

Query: 202 SQPLSVNIPDLTHPSIYHESKS----PLKNNATA---LNLAVISPSLEPHGMVRSTKRAR 261
           ++ LS  IP L+ PS+YHE  S     L  +A +   L+  V S       M+ +  R  
Sbjct: 123 TKVLSFTIPSLSKPSVYHEPSSIGSMALTESALSQHGLSKVVYSGPHPQREMLTAQNRFE 182

Query: 262 IVGVALELYYTKIDKIPETSKIEFCEFC 280
           ++   L  Y   +  +P  S    C+ C
Sbjct: 183 VLTFLLLCYNAALTYMPSVSLQSLCQIC 207

BLAST of Bhi01G001178 vs. ExPASy Swiss-Prot
Match: Q6P9N1 (Hyccin OS=Mus musculus OX=10090 GN=Fam126a PE=1 SV=3)

HSP 1 Score: 58.5 bits (140), Expect = 2.0e-07
Identity = 55/202 (27.23%), Postives = 88/202 (43.56%), Query Frame = 0

Query: 88  IESLKSILPNIPSSLPSAPNPALALLNDLETTAQITALLRRPTSGAGDDNLCRWLYDTFQ 147
           +E   S    +P +  S PN A  L +       +  +++ P S   +  +C  L++ ++
Sbjct: 9   VEEWLSEFKTLPET--SLPNYATNLKDKSSLVTSLYKVIQEPQSELLEP-VCHQLFEFYR 68

Query: 148 SNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLAG-FEAVLLSLYAHE--TNRRASQPLSV 207
           S    L    L+FLP L+  YL+   SR    +G  EA+LL +Y  E       S+ LS 
Sbjct: 69  SGEEQLLRFTLQFLPELMWCYLAVSASRDVHSSGCIEALLLGVYNLEIVDKHGHSKVLSF 128

Query: 208 NIPDLTHPSIYHESKS----PLKNNATA---LNLAVISPSLEPHGMVRSTKRARIVGVAL 267
            IP L+ PS+YHE  S     L  +A +   L+  V S       M+ +  R  ++   L
Sbjct: 129 TIPSLSKPSVYHEPSSIGSMALTESALSQHGLSKVVYSGPHPQREMLTAQNRFEVLTFLL 188

Query: 268 ELYYTKIDKIPETSKIEFCEFC 280
             Y   +  +P  S    C+ C
Sbjct: 189 LCYNAALTYMPSVSLQSLCQIC 207

BLAST of Bhi01G001178 vs. ExPASy Swiss-Prot
Match: Q8IXS8 (Protein FAM126B OS=Homo sapiens OX=9606 GN=FAM126B PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.4e-07
Identity = 48/166 (28.92%), Postives = 78/166 (46.99%), Query Frame = 0

Query: 138 LCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSR-RKSLAGFEAVLLSLYAHETNR 197
           +C  L++ ++S+   LK   L+FLP L+  YL   VSR R+S    EA+LL +Y  E   
Sbjct: 56  VCHQLFELYRSSEVRLKRFTLQFLPELMWVYLRLTVSRDRQSNGCIEALLLGIYNLEIAD 115

Query: 198 R--ASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEPHGMVR--------- 257
           +   ++ LS  IP L+ PSIYHE        +T  ++A+   +L  H ++R         
Sbjct: 116 KDGNNKVLSFTIPSLSKPSIYHEP-------STIGSMALTEGALCQHDLIRVVYSDLHPQ 175

Query: 258 -----STKRARIVGVALELYYTKIDKIPETSKIEFCEF-CRIWASG 286
                +  R  ++   +  Y + I  +P +S    C    R+  SG
Sbjct: 176 RETFTAQNRFEVLSFLMLCYNSAIVYMPASSYQSLCRMGSRVCVSG 214

BLAST of Bhi01G001178 vs. ExPASy Swiss-Prot
Match: Q5R977 (Protein FAM126B OS=Pongo abelii OX=9601 GN=FAM126B PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.4e-07
Identity = 48/166 (28.92%), Postives = 78/166 (46.99%), Query Frame = 0

Query: 138 LCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSR-RKSLAGFEAVLLSLYAHETNR 197
           +C  L++ ++S+   LK   L+FLP L+  YL   VSR R+S    EA+LL +Y  E   
Sbjct: 56  VCHQLFELYRSSEVRLKRFTLQFLPELMWVYLRLTVSRDRQSNGCIEALLLGIYNLEIAD 115

Query: 198 R--ASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEPHGMVR--------- 257
           +   ++ LS  IP L+ PSIYHE        +T  ++A+   +L  H ++R         
Sbjct: 116 KDGNNKVLSFTIPSLSKPSIYHEP-------STIGSMALTEGALCQHDLIRVVYSDLHPQ 175

Query: 258 -----STKRARIVGVALELYYTKIDKIPETSKIEFCEF-CRIWASG 286
                +  R  ++   +  Y + I  +P +S    C    R+  SG
Sbjct: 176 RETFTAQNRFEVLSFLMLCYNSAIVYMPASSYQSLCRMGSRVCVSG 214

BLAST of Bhi01G001178 vs. ExPASy Swiss-Prot
Match: Q6P121 (Hyccin OS=Danio rerio OX=7955 GN=fam126a PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.4e-07
Identity = 39/111 (35.14%), Postives = 60/111 (54.05%), Query Frame = 0

Query: 138 LCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLAG-FEAVLLSLYAHETNR 197
           +C  L++ ++S  P L+   L+FLP L+ +YLS   +R    +G  EA+LL +Y  E   
Sbjct: 56  VCHQLFEFYRSGEPRLQRFTLQFLPELVWSYLSVTAARDPHCSGCIEALLLGIYNLEIVD 115

Query: 198 R--ASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEPHGMVR 246
           +   S+ LS  IP L+ PS+YHE  S         +LA+   +L  HG+ R
Sbjct: 116 KDGQSKVLSFTIPSLSKPSVYHEPSS-------IGSLALTEGALANHGLSR 159

BLAST of Bhi01G001178 vs. NCBI nr
Match: XP_038879407.1 (uncharacterized protein LOC120071285 [Benincasa hispida])

HSP 1 Score: 763.1 bits (1969), Expect = 1.2e-216
Identity = 403/403 (100.00%), Postives = 403/403 (100.00%), Query Frame = 0

Query: 1   MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAAAEETTPTIAAAEAPVIKTS 60
           MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAAAEETTPTIAAAEAPVIKTS
Sbjct: 1   MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAAAEETTPTIAAAEAPVIKTS 60

Query: 61  SRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPNIPSSLPSAPNPALALLNDLETTA 120
           SRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPNIPSSLPSAPNPALALLNDLETTA
Sbjct: 61  SRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPNIPSSLPSAPNPALALLNDLETTA 120

Query: 121 QITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLA 180
           QITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLA
Sbjct: 121 QITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLA 180

Query: 181 GFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEP 240
           GFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEP
Sbjct: 181 GFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEP 240

Query: 241 HGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEEAAA 300
           HGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEEAAA
Sbjct: 241 HGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEEAAA 300

Query: 301 EEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAIRSLYLR 360
           EEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAIRSLYLR
Sbjct: 301 EEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAIRSLYLR 360

Query: 361 SMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           SMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL
Sbjct: 361 SMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 403

BLAST of Bhi01G001178 vs. NCBI nr
Match: XP_008456035.1 (PREDICTED: hyccin [Cucumis melo])

HSP 1 Score: 645.6 bits (1664), Expect = 2.9e-181
Identity = 358/409 (87.53%), Postives = 367/409 (89.73%), Query Frame = 0

Query: 2   SSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAA---AEETTPTIAAAEAPVIK 61
           SSSSSDDDSPAAVEPTPAEE A  ++P    ETAI+E AA   AEETTPTIAA EAPV K
Sbjct: 3   SSSSSDDDSPAAVEPTPAEETAENKEP----ETAIEEIAAPADAEETTPTIAAVEAPVTK 62

Query: 62  TSSRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPN-IPSSLPSAPNPALALLNDLE 121
           TSSRASGSGPVVRFDISQSSSLTTIA+TAIESL  ILPN IPSSL SAPNPALALLNDLE
Sbjct: 63  TSSRASGSGPVVRFDISQSSSLTTIAQTAIESLIPILPNTIPSSLSSAPNPALALLNDLE 122

Query: 122 TTAQITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRK 181
           T AQITALLRRPTSGAGDDNLCRWLYDTFQS+NPDLKLVVLRFLPVLL AYLSRVVSRRK
Sbjct: 123 TIAQITALLRRPTSGAGDDNLCRWLYDTFQSSNPDLKLVVLRFLPVLLSAYLSRVVSRRK 182

Query: 182 SLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPS 241
           SLAGFEAVLLSLYAHETNRRA QPLSVNIPDLTHPSIYHES SP KNNATALNLAVISPS
Sbjct: 183 SLAGFEAVLLSLYAHETNRRAGQPLSVNIPDLTHPSIYHESISPHKNNATALNLAVISPS 242

Query: 242 LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEE 301
           LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA  GD E   VK EE
Sbjct: 243 LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA--GDVENRGVKKEE 302

Query: 302 ---AAAEEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAI 361
              A  EEAEE+ IGRIPLPWE+LQPILRVLGHCLLGSN   KCKK E T LFDAAI AI
Sbjct: 303 ATVAVVEEAEEDGIGRIPLPWEILQPILRVLGHCLLGSNSIVKCKKKERTALFDAAIGAI 362

Query: 362 RSLYLRSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           RSLYLRSMHDINPKAILATGSLV+LGNMAMESADEIDYTEIPYQT+INL
Sbjct: 363 RSLYLRSMHDINPKAILATGSLVKLGNMAMESADEIDYTEIPYQTIINL 405

BLAST of Bhi01G001178 vs. NCBI nr
Match: KAA0038931.1 (hyccin [Cucumis melo var. makuwa] >TYK11229.1 hyccin [Cucumis melo var. makuwa])

HSP 1 Score: 642.5 bits (1656), Expect = 2.4e-180
Identity = 356/409 (87.04%), Postives = 365/409 (89.24%), Query Frame = 0

Query: 2   SSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAA---AEETTPTIAAAEAPVIK 61
           SSSS DDDSPAAVEPTPAEE A  ++P    ETAI+E AA   AEETTPTIAA EAPV K
Sbjct: 3   SSSSDDDDSPAAVEPTPAEETAENKEP----ETAIEEIAAPADAEETTPTIAAVEAPVTK 62

Query: 62  TSSRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPN-IPSSLPSAPNPALALLNDLE 121
           TSSRASGSGPVVRFDISQSSSLTTIA+TAIESL  ILPN IPSSL SAPNPALALLNDLE
Sbjct: 63  TSSRASGSGPVVRFDISQSSSLTTIAQTAIESLIPILPNTIPSSLSSAPNPALALLNDLE 122

Query: 122 TTAQITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRK 181
           T AQITALLRRPTSGAGDDNLCRWLYDTFQS+NPDLKLVVLRFLPVLL AYLSRVVSRRK
Sbjct: 123 TIAQITALLRRPTSGAGDDNLCRWLYDTFQSSNPDLKLVVLRFLPVLLSAYLSRVVSRRK 182

Query: 182 SLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPS 241
           SLAGFEAVLLSLYAHETNRRA QPLSVNIPDLTHPSIYHES SP KNNATALNL VISPS
Sbjct: 183 SLAGFEAVLLSLYAHETNRRAGQPLSVNIPDLTHPSIYHESISPHKNNATALNLVVISPS 242

Query: 242 LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEE 301
           LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA  GD E   VK EE
Sbjct: 243 LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA--GDVENRGVKKEE 302

Query: 302 ---AAAEEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAI 361
              A  EEAEE+ IGRIPLPWE+LQPILRVLGHCLLGSN   KCKK E T LFDAAI AI
Sbjct: 303 ATVAVVEEAEEDGIGRIPLPWEILQPILRVLGHCLLGSNSIVKCKKKERTALFDAAIGAI 362

Query: 362 RSLYLRSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           RSLYLRSMHDINPKAILATGSLV+LGNMAMESADEIDYTEIPYQT+INL
Sbjct: 363 RSLYLRSMHDINPKAILATGSLVKLGNMAMESADEIDYTEIPYQTIINL 405

BLAST of Bhi01G001178 vs. NCBI nr
Match: XP_004146279.1 (uncharacterized protein LOC101210037 [Cucumis sativus] >KGN57615.1 hypothetical protein Csa_011737 [Cucumis sativus])

HSP 1 Score: 634.0 bits (1634), Expect = 8.6e-178
Identity = 353/414 (85.27%), Postives = 363/414 (87.68%), Query Frame = 0

Query: 1   MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAA---AEETTPTIAAAEAPVI 60
           MSSSSS DD  AAVEPTPAEE A  ++P    ETAI+E AA   AEETTPTIAA EAPV 
Sbjct: 1   MSSSSSSDDDSAAVEPTPAEETAENKEP----ETAIEEIAAPAEAEETTPTIAAVEAPVT 60

Query: 61  KTSSRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPN-IPSSLPSAPNPALALLNDL 120
           KTSSRASGSGPVVRFDISQSSSLTTIA+TAIESLK ILPN IPSSL SAPNPALALLNDL
Sbjct: 61  KTSSRASGSGPVVRFDISQSSSLTTIAQTAIESLKPILPNTIPSSLSSAPNPALALLNDL 120

Query: 121 ETTAQITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRR 180
           ET AQITALLRRPTSGAGDDNLCRWLYDTFQS+NPDLKLVVLRFLPVLL AYLSRVVSRR
Sbjct: 121 ETIAQITALLRRPTSGAGDDNLCRWLYDTFQSSNPDLKLVVLRFLPVLLSAYLSRVVSRR 180

Query: 181 KSLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISP 240
           KSLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHES  P KNNATALNLAVISP
Sbjct: 181 KSLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESIFPHKNNATALNLAVISP 240

Query: 241 SLEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNE 300
           SLEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA  GD     VK E
Sbjct: 241 SLEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA--GDVNNRGVKKE 300

Query: 301 EAAA-------EEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDA 360
           EA A       EE EE+ IGRIPLPWE+LQPILRVLGHCLLGSN    CKK E T LFDA
Sbjct: 301 EATAAVVEEAEEEEEEDGIGRIPLPWEILQPILRVLGHCLLGSNSIVNCKKKERTALFDA 360

Query: 361 AIAAIRSLYLRSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           AI AIRSLYLRSMHDINPKAILATGSLV+LG+MAMES DEIDYTEIPYQT+INL
Sbjct: 361 AIGAIRSLYLRSMHDINPKAILATGSLVKLGDMAMESTDEIDYTEIPYQTIINL 408

BLAST of Bhi01G001178 vs. NCBI nr
Match: XP_023552628.1 (uncharacterized protein LOC111810220 [Cucurbita pepo subsp. pepo] >XP_023552646.1 uncharacterized protein LOC111810232 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 585.1 bits (1507), Expect = 4.6e-163
Identity = 316/403 (78.41%), Postives = 347/403 (86.10%), Query Frame = 0

Query: 1   MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAAAEETTPTIAAAEAPVIKTS 60
           MSSS +D+  PAA E T AEE A +E PTPA         +AEETTP   AA+ P  + +
Sbjct: 1   MSSSHADNGRPAAQETTTAEEAAGQE-PTPA--------VSAEETTP--PAAKQPTARHN 60

Query: 61  SRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPNIPSSLPSAPNPALALLNDLETTA 120
            R SGSG VVRFD+SQ++SLT+IA++AIESLK ILPNI ++L +APNPALALL+D E TA
Sbjct: 61  RRTSGSGLVVRFDVSQTASLTSIAQSAIESLKLILPNISAALSAAPNPALALLHDTEVTA 120

Query: 121 QITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLA 180
           QITALLR  TSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLP+LLGAYLSRVVSRRKSLA
Sbjct: 121 QITALLRSATSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPILLGAYLSRVVSRRKSLA 180

Query: 181 GFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEP 240
           GFEAVLLSLYAHETNRRASQPL+VNIPDL HPSIYHE+KSPLK NATALNLAVISPSLEP
Sbjct: 181 GFEAVLLSLYAHETNRRASQPLTVNIPDLAHPSIYHETKSPLKYNATALNLAVISPSLEP 240

Query: 241 HGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEEAAA 300
           HGMVRSTKRARIVGVALELYYTKI+KIPE+SKI+FCEFCR+WA G D+  G  K+E    
Sbjct: 241 HGMVRSTKRARIVGVALELYYTKIEKIPESSKIDFCEFCRLWAGGDDESGGAKKDEREEE 300

Query: 301 EEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAIRSLYLR 360
           EE EEE IG IPLPWE+LQPILRVLGHCLLGSN+  K KKNETTPLF AAIAAIRSLY+R
Sbjct: 301 EEEEEEDIGIIPLPWEILQPILRVLGHCLLGSNLITKSKKNETTPLFKAAIAAIRSLYVR 360

Query: 361 SMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           SMHDINPKAILATGSL+RLGNMAMES DE+DYTEIP QTVINL
Sbjct: 361 SMHDINPKAILATGSLMRLGNMAMESGDEVDYTEIPAQTVINL 392

BLAST of Bhi01G001178 vs. ExPASy TrEMBL
Match: A0A1S3C3J2 (hyccin OS=Cucumis melo OX=3656 GN=LOC103496087 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.4e-181
Identity = 358/409 (87.53%), Postives = 367/409 (89.73%), Query Frame = 0

Query: 2   SSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAA---AEETTPTIAAAEAPVIK 61
           SSSSSDDDSPAAVEPTPAEE A  ++P    ETAI+E AA   AEETTPTIAA EAPV K
Sbjct: 3   SSSSSDDDSPAAVEPTPAEETAENKEP----ETAIEEIAAPADAEETTPTIAAVEAPVTK 62

Query: 62  TSSRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPN-IPSSLPSAPNPALALLNDLE 121
           TSSRASGSGPVVRFDISQSSSLTTIA+TAIESL  ILPN IPSSL SAPNPALALLNDLE
Sbjct: 63  TSSRASGSGPVVRFDISQSSSLTTIAQTAIESLIPILPNTIPSSLSSAPNPALALLNDLE 122

Query: 122 TTAQITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRK 181
           T AQITALLRRPTSGAGDDNLCRWLYDTFQS+NPDLKLVVLRFLPVLL AYLSRVVSRRK
Sbjct: 123 TIAQITALLRRPTSGAGDDNLCRWLYDTFQSSNPDLKLVVLRFLPVLLSAYLSRVVSRRK 182

Query: 182 SLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPS 241
           SLAGFEAVLLSLYAHETNRRA QPLSVNIPDLTHPSIYHES SP KNNATALNLAVISPS
Sbjct: 183 SLAGFEAVLLSLYAHETNRRAGQPLSVNIPDLTHPSIYHESISPHKNNATALNLAVISPS 242

Query: 242 LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEE 301
           LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA  GD E   VK EE
Sbjct: 243 LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA--GDVENRGVKKEE 302

Query: 302 ---AAAEEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAI 361
              A  EEAEE+ IGRIPLPWE+LQPILRVLGHCLLGSN   KCKK E T LFDAAI AI
Sbjct: 303 ATVAVVEEAEEDGIGRIPLPWEILQPILRVLGHCLLGSNSIVKCKKKERTALFDAAIGAI 362

Query: 362 RSLYLRSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           RSLYLRSMHDINPKAILATGSLV+LGNMAMESADEIDYTEIPYQT+INL
Sbjct: 363 RSLYLRSMHDINPKAILATGSLVKLGNMAMESADEIDYTEIPYQTIINL 405

BLAST of Bhi01G001178 vs. ExPASy TrEMBL
Match: A0A5D3CLY5 (Hyccin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00740 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.2e-180
Identity = 356/409 (87.04%), Postives = 365/409 (89.24%), Query Frame = 0

Query: 2   SSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAA---AEETTPTIAAAEAPVIK 61
           SSSS DDDSPAAVEPTPAEE A  ++P    ETAI+E AA   AEETTPTIAA EAPV K
Sbjct: 3   SSSSDDDDSPAAVEPTPAEETAENKEP----ETAIEEIAAPADAEETTPTIAAVEAPVTK 62

Query: 62  TSSRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPN-IPSSLPSAPNPALALLNDLE 121
           TSSRASGSGPVVRFDISQSSSLTTIA+TAIESL  ILPN IPSSL SAPNPALALLNDLE
Sbjct: 63  TSSRASGSGPVVRFDISQSSSLTTIAQTAIESLIPILPNTIPSSLSSAPNPALALLNDLE 122

Query: 122 TTAQITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRK 181
           T AQITALLRRPTSGAGDDNLCRWLYDTFQS+NPDLKLVVLRFLPVLL AYLSRVVSRRK
Sbjct: 123 TIAQITALLRRPTSGAGDDNLCRWLYDTFQSSNPDLKLVVLRFLPVLLSAYLSRVVSRRK 182

Query: 182 SLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPS 241
           SLAGFEAVLLSLYAHETNRRA QPLSVNIPDLTHPSIYHES SP KNNATALNL VISPS
Sbjct: 183 SLAGFEAVLLSLYAHETNRRAGQPLSVNIPDLTHPSIYHESISPHKNNATALNLVVISPS 242

Query: 242 LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEE 301
           LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA  GD E   VK EE
Sbjct: 243 LEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA--GDVENRGVKKEE 302

Query: 302 ---AAAEEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAI 361
              A  EEAEE+ IGRIPLPWE+LQPILRVLGHCLLGSN   KCKK E T LFDAAI AI
Sbjct: 303 ATVAVVEEAEEDGIGRIPLPWEILQPILRVLGHCLLGSNSIVKCKKKERTALFDAAIGAI 362

Query: 362 RSLYLRSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           RSLYLRSMHDINPKAILATGSLV+LGNMAMESADEIDYTEIPYQT+INL
Sbjct: 363 RSLYLRSMHDINPKAILATGSLVKLGNMAMESADEIDYTEIPYQTIINL 405

BLAST of Bhi01G001178 vs. ExPASy TrEMBL
Match: A0A0A0L6M4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G229410 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.2e-178
Identity = 353/414 (85.27%), Postives = 363/414 (87.68%), Query Frame = 0

Query: 1   MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAA---AEETTPTIAAAEAPVI 60
           MSSSSS DD  AAVEPTPAEE A  ++P    ETAI+E AA   AEETTPTIAA EAPV 
Sbjct: 1   MSSSSSSDDDSAAVEPTPAEETAENKEP----ETAIEEIAAPAEAEETTPTIAAVEAPVT 60

Query: 61  KTSSRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPN-IPSSLPSAPNPALALLNDL 120
           KTSSRASGSGPVVRFDISQSSSLTTIA+TAIESLK ILPN IPSSL SAPNPALALLNDL
Sbjct: 61  KTSSRASGSGPVVRFDISQSSSLTTIAQTAIESLKPILPNTIPSSLSSAPNPALALLNDL 120

Query: 121 ETTAQITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRR 180
           ET AQITALLRRPTSGAGDDNLCRWLYDTFQS+NPDLKLVVLRFLPVLL AYLSRVVSRR
Sbjct: 121 ETIAQITALLRRPTSGAGDDNLCRWLYDTFQSSNPDLKLVVLRFLPVLLSAYLSRVVSRR 180

Query: 181 KSLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISP 240
           KSLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHES  P KNNATALNLAVISP
Sbjct: 181 KSLAGFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESIFPHKNNATALNLAVISP 240

Query: 241 SLEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNE 300
           SLEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA  GD     VK E
Sbjct: 241 SLEPHGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA--GDVNNRGVKKE 300

Query: 301 EAAA-------EEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDA 360
           EA A       EE EE+ IGRIPLPWE+LQPILRVLGHCLLGSN    CKK E T LFDA
Sbjct: 301 EATAAVVEEAEEEEEEDGIGRIPLPWEILQPILRVLGHCLLGSNSIVNCKKKERTALFDA 360

Query: 361 AIAAIRSLYLRSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           AI AIRSLYLRSMHDINPKAILATGSLV+LG+MAMES DEIDYTEIPYQT+INL
Sbjct: 361 AIGAIRSLYLRSMHDINPKAILATGSLVKLGDMAMESTDEIDYTEIPYQTIINL 408

BLAST of Bhi01G001178 vs. ExPASy TrEMBL
Match: A0A6J1E958 (uncharacterized protein LOC111430539 OS=Cucurbita moschata OX=3662 GN=LOC111430539 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.9e-162
Identity = 314/403 (77.92%), Postives = 346/403 (85.86%), Query Frame = 0

Query: 1   MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAAAEETTPTIAAAEAPVIKTS 60
           MSSS +D+  PA  E T A+E AA+E PTPA         +AEETTP   AA+ P  + +
Sbjct: 1   MSSSHADNGRPAGQETTTAQEAAAQE-PTPA--------VSAEETTP--PAAKQPTARHN 60

Query: 61  SRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPNIPSSLPSAPNPALALLNDLETTA 120
            R SGSG VVRFD+SQ++SLT+IA++AIESLK ILPNI S+L +APNPALALL+D E TA
Sbjct: 61  RRTSGSGLVVRFDVSQTASLTSIAQSAIESLKLILPNISSALSAAPNPALALLHDTEVTA 120

Query: 121 QITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLA 180
           QITALLR  TSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLP+LLGAYLSRVVSRRKSLA
Sbjct: 121 QITALLRSSTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPILLGAYLSRVVSRRKSLA 180

Query: 181 GFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEP 240
           GFEAVLLSLYAHETNRRASQPL+VNIPDL HPSIYHE+KSPLK NATALNLAVISPSLEP
Sbjct: 181 GFEAVLLSLYAHETNRRASQPLTVNIPDLAHPSIYHETKSPLKYNATALNLAVISPSLEP 240

Query: 241 HGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEEAAA 300
           HGMVRSTKRARIVGVALELYYTKI+KIPE+SKI+FCEFCR+WA G D+  G  K+E    
Sbjct: 241 HGMVRSTKRARIVGVALELYYTKIEKIPESSKIDFCEFCRLWAGGDDESGGAKKDEREEE 300

Query: 301 EEAEEESIGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAIRSLYLR 360
           EE EEE IG IPLPWE+LQPILRVLGHCLLGSN+  K KKNETTPLF AAI AIRSLY+R
Sbjct: 301 EEEEEEDIGIIPLPWEILQPILRVLGHCLLGSNLITKSKKNETTPLFKAAIGAIRSLYVR 360

Query: 361 SMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           SMHDINPKAILATGSL+RLGNMA+ES DE+DYTEIP QTVINL
Sbjct: 361 SMHDINPKAILATGSLMRLGNMAIESGDEVDYTEIPAQTVINL 392

BLAST of Bhi01G001178 vs. ExPASy TrEMBL
Match: A0A6J1J402 (uncharacterized protein LOC111483205 OS=Cucurbita maxima OX=3661 GN=LOC111483205 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.5e-162
Identity = 321/404 (79.46%), Postives = 348/404 (86.14%), Query Frame = 0

Query: 1   MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAAAEETTPTIAAAEAPVIKTS 60
           MSSS +D+  PAA E T AEE AA+E PTPA         +AEET P   AA+ P  + +
Sbjct: 1   MSSSPADNGRPAAQEITTAEEAAAQE-PTPA--------VSAEETNP--PAAKPPAARHN 60

Query: 61  SRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPNIPSSLPSAPNPALALLNDLETTA 120
            R SGSG VVRFDISQ++SLT+IA++AIESLK ILPNI S+L +APNPALALL+D E TA
Sbjct: 61  RRTSGSGLVVRFDISQTASLTSIAQSAIESLKLILPNISSALSAAPNPALALLHDTEVTA 120

Query: 121 QITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLA 180
           QI ALLR  TSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLP+LLGAYLSRVVSRRKSLA
Sbjct: 121 QIIALLRSSTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPILLGAYLSRVVSRRKSLA 180

Query: 181 GFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEP 240
           GFEAVLLSLYAHETNRRASQPL+VNIPDL HPSIYHE+KSPLK NATALNLAVISPSLEP
Sbjct: 181 GFEAVLLSLYAHETNRRASQPLTVNIPDLAHPSIYHETKSPLKYNATALNLAVISPSLEP 240

Query: 241 HGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWASGGDDEKGKVKNEEAAA 300
           HGMVRSTKRARIVGVALELYYTKI+KIPE+SKI+FCEFCR+WA GGDDE G  K EE   
Sbjct: 241 HGMVRSTKRARIVGVALELYYTKIEKIPESSKIDFCEFCRLWA-GGDDESGGAKKEEREE 300

Query: 301 EEAEEES-IGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNETTPLFDAAIAAIRSLYL 360
           EE EEE  IG IPLPWE+LQPILRVLGHCLLGSN+  K KKNET PLF+AAIAAIRSLY+
Sbjct: 301 EEEEEEEYIGIIPLPWEILQPILRVLGHCLLGSNLITKSKKNETRPLFNAAIAAIRSLYV 360

Query: 361 RSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 404
           RSMHDINPKAILATGSL+RLGNMAMES DEIDYTEIP QTVINL
Sbjct: 361 RSMHDINPKAILATGSLMRLGNMAMESGDEIDYTEIPAQTVINL 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G21050.16.5e-9152.20LOCATED IN: chloroplast; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 8 ... [more]
AT5G64090.13.6e-5737.97FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
Match NameE-valueIdentityDescription
Q9BYI35.2e-0826.92Hyccin OS=Homo sapiens OX=9606 GN=FAM126A PE=1 SV=2[more]
Q6P9N12.0e-0727.23Hyccin OS=Mus musculus OX=10090 GN=Fam126a PE=1 SV=3[more]
Q8IXS83.4e-0728.92Protein FAM126B OS=Homo sapiens OX=9606 GN=FAM126B PE=1 SV=1[more]
Q5R9773.4e-0728.92Protein FAM126B OS=Pongo abelii OX=9601 GN=FAM126B PE=2 SV=1[more]
Q6P1213.4e-0735.14Hyccin OS=Danio rerio OX=7955 GN=fam126a PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038879407.11.2e-216100.00uncharacterized protein LOC120071285 [Benincasa hispida][more]
XP_008456035.12.9e-18187.53PREDICTED: hyccin [Cucumis melo][more]
KAA0038931.12.4e-18087.04hyccin [Cucumis melo var. makuwa] >TYK11229.1 hyccin [Cucumis melo var. makuwa][more]
XP_004146279.18.6e-17885.27uncharacterized protein LOC101210037 [Cucumis sativus] >KGN57615.1 hypothetical ... [more]
XP_023552628.14.6e-16378.41uncharacterized protein LOC111810220 [Cucurbita pepo subsp. pepo] >XP_023552646.... [more]
Match NameE-valueIdentityDescription
A0A1S3C3J21.4e-18187.53hyccin OS=Cucumis melo OX=3656 GN=LOC103496087 PE=3 SV=1[more]
A0A5D3CLY51.2e-18087.04Hyccin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00740 PE=3 SV... [more]
A0A0A0L6M44.2e-17885.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G229410 PE=3 SV=1[more]
A0A6J1E9581.9e-16277.92uncharacterized protein LOC111430539 OS=Cucurbita moschata OX=3662 GN=LOC1114305... [more]
A0A6J1J4022.5e-16279.46uncharacterized protein LOC111483205 OS=Cucurbita maxima OX=3661 GN=LOC111483205... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 286..306
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..68
NoneNo IPR availablePANTHERPTHR31220:SF10HYCCINcoord: 74..402
IPR018619HyccinPFAMPF09790Hyccincoord: 107..379
e-value: 3.9E-54
score: 184.0
IPR018619HyccinPANTHERPTHR31220HYCCIN RELATEDcoord: 74..402

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M001178Bhi01M001178mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046854 phosphatidylinositol phosphate biosynthetic process
biological_process GO:0072659 protein localization to plasma membrane
cellular_component GO:0005829 cytosol
cellular_component GO:0005886 plasma membrane