Bhi01G000901 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G000901
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	chr1: 25015837 .. 25017014 (+)
RNA-Seq Expression	Bhi01G000901
Synteny	Bhi01G000901

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACGTGTCTTCCACAACTATAAATATAGTAAAAATCTCCCGGCAACCCTAATAGATTCACTAGAGAAAGAGATGGAATCTCAAATCCTAAAATCCTCACTAACCCTTTTCTTCTTCATTGTTTTAACAACATTCACTACCTCCGCCGCGGCCTCTTCTTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCATGCAAGCCGACGCCATATCCGCGCCTTTGCGAGACTGCACTCTCTTTATACGCTTCACAGACCAAAAGAAACCAGCAAGAACTATGCAGGGCGGCCATGGTTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGATCTCCCGTCGGAAGTTGTCACCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCCGTCGACGAGATCCGTCGAGCTACCACTGCCATTAAAAAATTGAGTCGTTCTAAGGATGTGGATTTTCAGGTTGTTAAAACTACTACTTAATCCTATTAACGGTTTAATTTCATATTCTTTTTAGGTTAAAAAACATTTTTGTCTCTGAATTTTCGGGAAAATAATTAACAATTTTAGTTGATAATGATGTGACCCCTATATTTTTAAATTTGTAATTCTTGATTTTTAGTCCTTATGATCTATAATTTGTAATGACTTAGTCCTATCGTGAAAAAAACATTCATAAGGACTAAAACATTACAGATGTTTTTAGAGACTAAACCGTTTCTTTCATAAAAGTAAATGTGTTTAACCAAATTTATTCATATTCTTAATGCAGTTGAGTAGTATAAAGACGTGGATGAGCGCAGCAGAGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGCGGCGGGGCGGAAGGTGAGGACGAAGGTGAAGAAAGAGGTGAAGAATTGTAGTGTGATTGTGGTAAGGCAGATTAGTAATGCCTTGTCTCTCATCAACAACTTCAACTACAAACACTGAGCTTCTGAGTTTCTGAATTTACTACTTCAATGGTCACCTATCAAATACAAAATTTGTACTTCCAACTTTATCCATTTCAAACTATAAAATATGTTTCCTTTTCTGTGTTCTTTCATCATCTCAATTCTTTCTATAATTTTTTATGTCTATTTTGGTTATACATTATTACAATTTTACTTTAATTTGAT

mRNA sequence

GACGTGTCTTCCACAACTATAAATATAGTAAAAATCTCCCGGCAACCCTAATAGATTCACTAGAGAAAGAGATGGAATCTCAAATCCTAAAATCCTCACTAACCCTTTTCTTCTTCATTGTTTTAACAACATTCACTACCTCCGCCGCGGCCTCTTCTTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCATGCAAGCCGACGCCATATCCGCGCCTTTGCGAGACTGCACTCTCTTTATACGCTTCACAGACCAAAAGAAACCAGCAAGAACTATGCAGGGCGGCCATGGTTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGATCTCCCGTCGGAAGTTGTCACCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCCGTCGACGAGATCCGTCGAGCTACCACTGCCATTAAAAAATTGAGTCGTTCTAAGGATGTGGATTTTCAGTTGAGTAGTATAAAGACGTGGATGAGCGCAGCAGAGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGCGGCGGGGCGGAAGGTGAGGACGAAGGTGAAGAAAGAGGTGAAGAATTGTAGTGTGATTGTGGTAAGGCAGATTAGTAATGCCTTGTCTCTCATCAACAACTTCAACTACAAACACTGAGCTTCTGAGTTTCTGAATTTACTACTTCAATGGTCACCTATCAAATACAAAATTTGTACTTCCAACTTTATCCATTTCAAACTATAAAATATGTTTCCTTTTCTGTGTTCTTTCATCATCTCAATTCTTTCTATAATTTTTTATGTCTATTTTGGTTATACATTATTACAATTTTACTTTAATTTGAT

Coding sequence (CDS)

ATGGAATCTCAAATCCTAAAATCCTCACTAACCCTTTTCTTCTTCATTGTTTTAACAACATTCACTACCTCCGCCGCGGCCTCTTCTTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCATGCAAGCCGACGCCATATCCGCGCCTTTGCGAGACTGCACTCTCTTTATACGCTTCACAGACCAAAAGAAACCAGCAAGAACTATGCAGGGCGGCCATGGTTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGATCTCCCGTCGGAAGTTGTCACCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCCGTCGACGAGATCCGTCGAGCTACCACTGCCATTAAAAAATTGAGTCGTTCTAAGGATGTGGATTTTCAGTTGAGTAGTATAAAGACGTGGATGAGCGCAGCAGAGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGCGGCGGGGCGGAAGGTGAGGACGAAGGTGAAGAAAGAGGTGAAGAATTGTAGTGTGATTGTGGTAAGGCAGATTAGTAATGCCTTGTCTCTCATCAACAACTTCAACTACAAACACTGA

Protein sequence

MESQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYKH

Homology

[Close]

BLAST of Bhi01G000901 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 4.2e-22
Identity = 70/202 (34.65%), Postives = 112/202 (55.45%), Query Frame = 0


Query: 1   MESQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLY 60
           M  Q L   L++ + + L   T + AAS +   +     I+ +CK T YP LC  +LS+Y
Sbjct: 1   MAKQYLFVLLSISYLLSL-ELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVY 60

Query: 61  ASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRK-LSPYEAEVIGDCIDNLNDSVDE 120
           A+  + + + L   A+  +L  AQ+    +S+++R K L   E E I DC++ +ND+VD 
Sbjct: 61  ANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDR 120

Query: 121 IRRATTAIKKLSRSKDVD---FQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKE 180
           + ++   +K    +KD D   + +S+ +TW SAA TD  TC+DG S   GR +  ++K  
Sbjct: 121 LTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFS---GRVMDGRIKNS 180

Query: 181 VKNCSVIVVRQISNALSLINNF 199
           V+   + V  + SNALSLIN F
Sbjct: 181 VRARIMNVGHETSNALSLINAF 198

[Close]

BLAST of Bhi01G000901 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.4e-20
Identity = 62/193 (32.12%), Postives = 111/193 (57.51%), Query Frame = 0


Query: 10  LTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQ 69
           LT F  I+          ++++T       ++ +C  T YPRLC ++LS +AS  + + +
Sbjct: 14  LTTFLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPK 73

Query: 70  ELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKK 129
            +  AA+  +L +A+  ++++ ++S  +L P E   + DC++ L D+++E+R++   + +
Sbjct: 74  LMAHAALNITLASAKVTSAMMVRLSNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQ 133

Query: 130 LSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQIS 189
           LS S + +  +S I+TW+SAA TDV TCTDG     G  +  KVK  V+   +++    S
Sbjct: 134 LSGS-NYEVYISDIQTWVSAALTDVNTCTDGFE---GEDMDGKVKVLVRGRILVIAHLTS 193

Query: 190 NALSLINNFNYKH 203
           NAL+LIN+F   H
Sbjct: 194 NALALINHFASIH 202

[Close]

BLAST of Bhi01G000901 vs. TAIR 10
Match: AT5G51520.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 4.0e-20
Identity = 67/208 (32.21%), Postives = 113/208 (54.33%), Query Frame = 0


Query: 3   SQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYAS 62
           SQ+L  SLT+ F    +T     + S+SS  +  +  ++ AC  T YP  C  +LS Y+S
Sbjct: 2   SQVL-YSLTIVFVFFASTNIQKTSGSASSYSQNHKTFVKTACNSTTYPDKCYKSLSSYSS 61

Query: 63  QTKRNQQELCRAAMVSSLKAAQNATSIISKI----------SRRKLSPYEAEVIGDCIDN 122
             K +  +LC  A+  ++K+A+ ATS++SK+           + K+ P EA ++ DC++ 
Sbjct: 62  NIKSDPIKLCTTALNLNVKSAKEATSVVSKLLKMSQKSTAGRKGKMLP-EALILKDCLEE 121

Query: 123 LNDSVDEIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTK 182
           + D++ E+++A T +K L     +   +++++TW+S+A TD  TCTDG       KV  +
Sbjct: 122 MKDTIIELKQAITEMKNLQDGGSMAEHITNVRTWVSSALTDEGTCTDGFEEV---KVNKE 181

Query: 183 VKKEVKNCSVIVVRQISNALSLINNFNY 201
            KK+V      +    SN L+LI N  Y
Sbjct: 182 TKKKVNKVVEELATTTSNTLALITNLRY 204

[Close]

BLAST of Bhi01G000901 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 92.8 bits (229), Expect = 3.3e-19
Identity = 66/207 (31.88%), Postives = 111/207 (53.62%), Query Frame = 0


Query: 1   MESQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLY 60
           ME ++      L  F+ L   +T A  SSS         I  +C+ T Y  LC   L+ +
Sbjct: 1   MEPKLTHLCYCLLLFLPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAF 60

Query: 61  ASQTKRNQQELCRAAMVSSLKAAQNATSIISKISR-RKLSPYEAEVIGDCIDNLNDSVDE 120
           A + +RN+ +L + A+  +L   Q+ T  + K+++ R++   E   + DC++NL D ++ 
Sbjct: 61  ADKIRRNENQLAQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEM 120

Query: 121 IRRATTAIKKLSRS-KDVD---FQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKK 180
           + ++   +K++ RS +D D   ++LS+++TW+SAA TD  TC DG  G     V   VK 
Sbjct: 121 LAQSMRELKQVGRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGV---VKS 180

Query: 181 EVKNCSVIVVRQISNALSLINNFNYKH 203
            ++   V V R  SNAL+L+N F  +H
Sbjct: 181 AIRRRVVHVARVTSNALALVNRFAARH 204

[Close]

BLAST of Bhi01G000901 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 3.7e-18
Identity = 60/195 (30.77%), Postives = 104/195 (53.33%), Query Frame = 0


Query: 12  LFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQEL 71
           + F + L+T     A +           I+ +C+ T YP  C  +LS YAS+ K+  ++L
Sbjct: 10  ILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKL 69

Query: 72  CRAAMVSSLKAAQNATSIISKISRRK-LSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKL 131
              A+  S+  A++A + +S+++  K ++  + E + DC++ + D+VD +  +   +K L
Sbjct: 70  AETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHL 129

Query: 132 SR---SKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQ 191
                 +D  F LS+++TW SAA TD   C DG  G A   +  ++K  ++   V V  +
Sbjct: 130 EEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKA---MAGELKSLIRTHIVSVAEE 189

Query: 192 ISNALSLINNFNYKH 203
            SNAL+LIN+F  KH
Sbjct: 190 TSNALALINDFASKH 201

[Close]

BLAST of Bhi01G000901 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.9e-23
Identity = 73/198 (36.87%), Postives = 110/198 (55.56%), Query Frame = 0


Query: 8   SSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRN 67
           S  TL F  +L     ++AA       P    I+ +C  T YP +CE +LS YA   + N
Sbjct: 4   SKSTLVFSALLVILAAASAA-------PANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNN 63

Query: 68  QQELCRAAMVSSLKAAQNATSIISKISRRK-LSPYEAEVIGDCIDNLNDSVDEIRRATTA 127
            QEL   A+  SL   Q A + + ++++ K L   +   I DC++ + DS+D + R+   
Sbjct: 64  PQELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDE 123

Query: 128 IKKLSRSK--DVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIV 187
           +K LS +K  D  F++S+++TW+SAA TD  TC DG    AG+ +  K+K+ V+   V V
Sbjct: 124 MKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGF---AGKGMDGKIKESVRAQVVAV 183

Query: 188 VRQISNALSLINNFNYKH 203
            R  SNAL+L+NNF  KH
Sbjct: 184 ARVTSNALALVNNFAAKH 191

[Close]

BLAST of Bhi01G000901 vs. ExPASy Swiss-Prot
Match: Q9SB38 (Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-19
Identity = 61/193 (31.61%), Postives = 107/193 (55.44%), Query Frame = 0


Query: 9   SLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQ 68
           SLTL  FI  + F    AA+   T +    +++ AC  T YP +C   LS Y+S  K + 
Sbjct: 8   SLTLMVFINSSNF-PKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSDP 67

Query: 69  QELCRAAMVSSLKAAQNATSIISKI--SRRKLSPYEAEVIGDCIDNLNDSVDEIRRATTA 128
            +LC  ++  ++K+A+NAT ++S +    +    +E  ++ DC+D + D++DE+++A   
Sbjct: 68  IKLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVAE 127

Query: 129 IKKL-SRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVV 188
           +K +    K  +  L ++KTW+S+A TD  TCTDG       +V  + KK+VK     + 
Sbjct: 128 MKYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKKAISELS 187

Query: 189 RQISNALSLINNF 199
           +  SN L+L+ ++
Sbjct: 188 KTTSNTLALLTHY 196

[Close]

BLAST of Bhi01G000901 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.4e-17
Identity = 67/197 (34.01%), Postives = 111/197 (56.35%), Query Frame = 0


Query: 14  FFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTK-RNQQELC 73
           F ++L + T   ++S++         I  +C+ T YP LC   LS YA++ +  N Q+L 
Sbjct: 8   FLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLA 67

Query: 74  RAAMVSSLKAAQNATSIISKISRR--KLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKL 133
           + A++ SL  A++ T  ++K+++   K    E   I DCI+ L +SVD   R   ++K+L
Sbjct: 68  QTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVD---RLAQSVKEL 127

Query: 134 SR------SKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIV 193
           +R      S+D  +++S+++TW+SAA TD  TC DG S    R +  KVK+ ++   V V
Sbjct: 128 ARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFS---ERAMGGKVKRLIRYKVVHV 187

Query: 194 VRQISNALSLINNFNYK 202
            +  SNAL+L+N F  K
Sbjct: 188 AQVTSNALALVNQFAEK 198

[Close]

BLAST of Bhi01G000901 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.2e-17
Identity = 60/195 (30.77%), Postives = 104/195 (53.33%), Query Frame = 0


Query: 12  LFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQEL 71
           + F + L+T     A +           I+ +C+ T YP  C  +LS YAS+ K+  ++L
Sbjct: 10  ILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKL 69

Query: 72  CRAAMVSSLKAAQNATSIISKISRRK-LSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKL 131
              A+  S+  A++A + +S+++  K ++  + E + DC++ + D+VD +  +   +K L
Sbjct: 70  AETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHL 129

Query: 132 SR---SKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQ 191
                 +D  F LS+++TW SAA TD   C DG  G A   +  ++K  ++   V V  +
Sbjct: 130 EEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKA---MAGELKSLIRTHIVSVAEE 189

Query: 192 ISNALSLINNFNYKH 203
            SNAL+LIN+F  KH
Sbjct: 190 TSNALALINDFASKH 201

[Close]

BLAST of Bhi01G000901 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 5.8e-16
Identity = 63/198 (31.82%), Postives = 107/198 (54.04%), Query Frame = 0


Query: 11  TLFFFIVLTTFTT--SAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQ 70
           TLF F++ T   T  S+A  ++ T +     I+ +CK T YP +C  +L+ YA+  + + 
Sbjct: 8   TLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSP 67

Query: 71  QELCRAAMVSSLKAAQNATSIISKISR-RKLSPYEAEVIGDCIDNLNDSVDEIRRATTAI 130
           + L   A+  ++  AQ+    + ++ R   L   E + + DCI+ ++D+VD +  +   +
Sbjct: 68  RRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEV 127

Query: 131 KKLSRSKDVD---FQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIV 190
           K    +K  D   F +S+ +TW SAA T+  TC+DG    AGR +  +VK  V+   + +
Sbjct: 128 KMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGF---AGRVMDGRVKNSVRARILNL 187

Query: 191 VRQISNALSLINNFNYKH 203
            R  SNAL+LIN F  K+
Sbjct: 188 GRGTSNALALINAFAKKY 202

[Close]

BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_038882390.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 377.9 bits (969), Expect = 5.6e-101
Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 1   MESQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLY 60
           MESQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLY
Sbjct: 1   MESQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLY 60

Query: 61  ASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEI 120
           ASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEI
Sbjct: 61  ASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEI 120

Query: 121 RRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNC 180
           RRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNC
Sbjct: 121 RRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNC 180

Query: 181 SVIVVRQISNALSLINNFNYKH 203
           SVIVVRQISNALSLINNFNYKH
Sbjct: 181 SVIVVRQISNALSLINNFNYKH 202

[Close]

BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_004148137.1 (21 kDa protein [Cucumis sativus] >KGN57278.1 hypothetical protein Csa_010411 [Cucumis sativus])

HSP 1 Score: 330.5 bits (846), Expect = 1.0e-86
Identity = 177/203 (87.19%), Postives = 189/203 (93.10%), Query Frame = 0


Query: 1   MESQILKSSLTL--FFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALS 60
           MESQILKSSLTL  FFFI+LTTFT SA +SSSSTVRPVQPHIRKACKPTPYPRLCETALS
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVD 120
           LYASQTKRNQQELCRAAMVSSLKAAQNATSIISK+SRRK+S YEAEVIGDCIDNL DSVD
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 EIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVK 180
           E+RRA+TAIK LSRSKDVDFQL+SIKTWMSAA+TDVITCTDGLSG +G KV   +KK+VK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 NCSVIVVRQISNALSLINNFNYK 202
           NCS+ VVRQISNAL LINNFNYK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]

BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_008439099.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 329.7 bits (844), Expect = 1.7e-86
Identity = 177/202 (87.62%), Postives = 188/202 (93.07%), Query Frame = 0


Query: 1   MESQILKSSLTL-FFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSL 60
           MESQILKSSLTL FFFI+LTTFT SA ASSSSTVRPV+PHIRKACKPTPYPRLCETALSL
Sbjct: 1   MESQILKSSLTLFFFFIILTTFTPSAVASSSSTVRPVKPHIRKACKPTPYPRLCETALSL 60

Query: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDE 120
           YASQTKRNQQELCRAAMVSSLKAAQNATSIISK+SRRK+S YE EVIGDCID+L DSVDE
Sbjct: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDE 120

Query: 121 IRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKN 180
           +RRA+TAIK LSRSKDVDFQL+SIKTW SAA TD+ITCTDGLSG +G KV  KVKKEVKN
Sbjct: 121 LRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKN 180

Query: 181 CSVIVVRQISNALSLINNFNYK 202
           CS+ VVRQISNALSLINNFNYK
Sbjct: 181 CSINVVRQISNALSLINNFNYK 202

[Close]

BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_023553135.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 292.7 bits (748), Expect = 2.4e-75
Identity = 157/203 (77.34%), Postives = 176/203 (86.70%), Query Frame = 0


Query: 6   LKSSLTLFFFIVLTTFTTSAAASSSSTV-------RPVQPHIRKACKPTPYPRLCETALS 65
           +KS L LFFFI+  TFTTSA ASSSS V       +PV+PHIRKAC+PTPYPRLCETALS
Sbjct: 1   MKSLLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALS 60

Query: 66  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVD 125
           LY+SQTKR QQELCRAA+VSSLKAAQNATSIISK+SRRK+SPYEAEVIGDCIDNL DSV 
Sbjct: 61  LYSSQTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSVY 120

Query: 126 EIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVK 185
           E+RRATTAIK L+R+ DV+FQLSSIKTW+SA ETD++TCTDGLSG AG KVR+ VKKEVK
Sbjct: 121 ELRRATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVK 180

Query: 186 NCSVIVVRQISNALSLINNFNYK 202
            CSV V+RQ SNALSLI+N  YK
Sbjct: 181 KCSVNVIRQTSNALSLISNLKYK 203

[Close]

BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_022985512.1 (pectinesterase inhibitor 4-like [Cucurbita maxima])

HSP 1 Score: 292.0 bits (746), Expect = 4.0e-75
Identity = 151/196 (77.04%), Postives = 175/196 (89.29%), Query Frame = 0


Query: 6   LKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTK 65
           +KS L LFFFI++ TFT SA A+SSS ++PV+PHIRKAC+PTPYPRLCETALSLY+SQTK
Sbjct: 1   MKSLLILFFFILIATFTISAVAASSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQTK 60

Query: 66  RNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEIRRATT 125
           R QQELCRAA+VSSLKAAQNATSIISK+S RK+SPYEAEVIGDC DNL DS+ E+RRATT
Sbjct: 61  RTQQELCRAAVVSSLKAAQNATSIISKLSHRKVSPYEAEVIGDCTDNLKDSIYELRRATT 120

Query: 126 AIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVV 185
           AIK L+R+ DV+FQLSSIKTW+SAAET+++TCTDGLSG AG KVR+ VKKEVK CSV V+
Sbjct: 121 AIKSLNRADDVEFQLSSIKTWLSAAETNIMTCTDGLSGGAGWKVRSMVKKEVKKCSVNVI 180

Query: 186 RQISNALSLINNFNYK 202
           RQ SNALSLI+N  YK
Sbjct: 181 RQTSNALSLISNLKYK 196

[Close]

BLAST of Bhi01G000901 vs. ExPASy TrEMBL
Match: A0A0A0L671 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 5.0e-87
Identity = 177/203 (87.19%), Postives = 189/203 (93.10%), Query Frame = 0


Query: 1   MESQILKSSLTL--FFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALS 60
           MESQILKSSLTL  FFFI+LTTFT SA +SSSSTVRPVQPHIRKACKPTPYPRLCETALS
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVD 120
           LYASQTKRNQQELCRAAMVSSLKAAQNATSIISK+SRRK+S YEAEVIGDCIDNL DSVD
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 EIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVK 180
           E+RRA+TAIK LSRSKDVDFQL+SIKTWMSAA+TDVITCTDGLSG +G KV   +KK+VK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 NCSVIVVRQISNALSLINNFNYK 202
           NCS+ VVRQISNAL LINNFNYK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]

BLAST of Bhi01G000901 vs. ExPASy TrEMBL
Match: A0A1S3AXZ9 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 8.5e-87
Identity = 177/202 (87.62%), Postives = 188/202 (93.07%), Query Frame = 0


Query: 1   MESQILKSSLTL-FFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSL 60
           MESQILKSSLTL FFFI+LTTFT SA ASSSSTVRPV+PHIRKACKPTPYPRLCETALSL
Sbjct: 1   MESQILKSSLTLFFFFIILTTFTPSAVASSSSTVRPVKPHIRKACKPTPYPRLCETALSL 60

Query: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDE 120
           YASQTKRNQQELCRAAMVSSLKAAQNATSIISK+SRRK+S YE EVIGDCID+L DSVDE
Sbjct: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDE 120

Query: 121 IRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKN 180
           +RRA+TAIK LSRSKDVDFQL+SIKTW SAA TD+ITCTDGLSG +G KV  KVKKEVKN
Sbjct: 121 LRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKN 180

Query: 181 CSVIVVRQISNALSLINNFNYK 202
           CS+ VVRQISNALSLINNFNYK
Sbjct: 181 CSINVVRQISNALSLINNFNYK 202

[Close]

BLAST of Bhi01G000901 vs. ExPASy TrEMBL
Match: A0A6J1J533 (pectinesterase inhibitor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483499 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 2.0e-75
Identity = 151/196 (77.04%), Postives = 175/196 (89.29%), Query Frame = 0


Query: 6   LKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTK 65
           +KS L LFFFI++ TFT SA A+SSS ++PV+PHIRKAC+PTPYPRLCETALSLY+SQTK
Sbjct: 1   MKSLLILFFFILIATFTISAVAASSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQTK 60

Query: 66  RNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEIRRATT 125
           R QQELCRAA+VSSLKAAQNATSIISK+S RK+SPYEAEVIGDC DNL DS+ E+RRATT
Sbjct: 61  RTQQELCRAAVVSSLKAAQNATSIISKLSHRKVSPYEAEVIGDCTDNLKDSIYELRRATT 120

Query: 126 AIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVV 185
           AIK L+R+ DV+FQLSSIKTW+SAAET+++TCTDGLSG AG KVR+ VKKEVK CSV V+
Sbjct: 121 AIKSLNRADDVEFQLSSIKTWLSAAETNIMTCTDGLSGGAGWKVRSMVKKEVKKCSVNVI 180

Query: 186 RQISNALSLINNFNYK 202
           RQ SNALSLI+N  YK
Sbjct: 181 RQTSNALSLISNLKYK 196

[Close]

BLAST of Bhi01G000901 vs. ExPASy TrEMBL
Match: A0A6J1E4R7 (pectinesterase inhibitor 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430766 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 3.1e-73
Identity = 152/203 (74.88%), Postives = 174/203 (85.71%), Query Frame = 0


Query: 6   LKSSLTLFFFIVLTTFTT-------SAAASSSSTVRPVQPHIRKACKPTPYPRLCETALS 65
           +KS L LFFFI+  TFTT       SA  SSSS ++PV+PHI+KAC+PTPYPRLCETALS
Sbjct: 1   MKSLLILFFFILTATFTTSAVPSSPSAVPSSSSAIQPVKPHIKKACRPTPYPRLCETALS 60

Query: 66  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVD 125
           LY+SQTK  QQELCRAA+VSSLKAAQNATSIISK+SRRK+SPYEAEVIGDCIDNL DSV 
Sbjct: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSVY 120

Query: 126 EIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVK 185
           E+RRATTAIK L+R+ +V+FQLSSIKTW+SAA TD++TCTDGLSG AG KVR+ VKKEVK
Sbjct: 121 ELRRATTAIKSLNRANNVEFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180

Query: 186 NCSVIVVRQISNALSLINNFNYK 202
            CSV V+RQ SNALSLI+N  YK
Sbjct: 181 KCSVNVIRQTSNALSLISNLKYK 203

[Close]

BLAST of Bhi01G000901 vs. ExPASy TrEMBL
Match: A0A6J1FQ13 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111447758 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.5e-67
Identity = 142/201 (70.65%), Postives = 165/201 (82.09%), Query Frame = 0


Query: 1   MESQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLY 60
           ME QILKS LTLFFF+ + T TTS  ASSSS  RPV+P+I KACK TPYPRLCE +L+L+
Sbjct: 1   MEFQILKSLLTLFFFVSI-TITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLH 60

Query: 61  ASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEI 120
           ASQTKR QQE CRA M SSLK  ++ATSIISK+SR+K+S YEA+VI DC+DNL DS DE+
Sbjct: 61  ASQTKRTQQEFCRATMTSSLKTTRDATSIISKLSRQKISTYEAKVIRDCVDNLKDSTDEL 120

Query: 121 RRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNC 180
           RRA  AIK LSR+KD +FQL SIKTWMSAAETD +TC+DGLSG AGRKVR+KVK+EVK  
Sbjct: 121 RRAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKS 180

Query: 181 SVIVVRQISNALSLINNFNYK 202
             +VVR ISNALSL+N F YK
Sbjct: 181 VAVVVRHISNALSLLNTFKYK 200

The following BLAST results are available for this feature:

BLAST of Bhi01G000901 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62350.1	4.2e-22	34.65	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62360.1	1.4e-20	32.12	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G51520.1	4.0e-20	32.21	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	3.3e-19	31.88	pectin methylesterase inhibitor 1	[more]
AT4G25260.1	3.7e-18	30.77	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Bhi01G000901 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	2.9e-23	36.87	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB38	1.9e-19	31.61	Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1	[more]
Q9SI72	1.4e-17	34.01	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SB37	5.2e-17	30.77	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9STY5	5.8e-16	31.82	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]

BLAST of Bhi01G000901 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882390.1	5.6e-101	100.00	21 kDa protein-like [Benincasa hispida]	[more]
XP_004148137.1	1.0e-86	87.19	21 kDa protein [Cucumis sativus] >KGN57278.1 hypothetical protein Csa_010411 [Cu...	[more]
XP_008439099.1	1.7e-86	87.62	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
XP_023553135.1	2.4e-75	77.34	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022985512.1	4.0e-75	77.04	pectinesterase inhibitor 4-like [Cucurbita maxima]	[more]

BLAST of Bhi01G000901 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L671	5.0e-87	87.19	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 S...	[more]
A0A1S3AXZ9	8.5e-87	87.62	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1	[more]
A0A6J1J533	2.0e-75	77.04	pectinesterase inhibitor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483499 PE=4...	[more]
A0A6J1E4R7	3.1e-73	74.88	pectinesterase inhibitor 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430766 PE...	[more]
A0A6J1FQ13	1.5e-67	70.65	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111447758 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 35..194 e-value: 2.3E-35 score: 133.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 7..197 e-value: 9.6E-31 score: 104.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 40..194 e-value: 9.3E-29 score: 100.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 38..198 e-value: 3.2E-31 score: 110.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 38..198
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 8..198
None	No IPR available	PANTHER	PTHR31080:SF15	PECTINESTERASE INHIBITOR 4	coord: 8..198
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 43..198 e-value: 6.47424E-38 score: 126.784

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M000901	Bhi01M000901	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity