Bhi01G000900 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G000900
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	chr1: 24992330 .. 24992914 (-)
RNA-Seq Expression	Bhi01G000900
Synteny	Bhi01G000900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGTCTCAAATTCCAAGAAAATTTTGATTCTTTTCCAATTAATTCTCCAATTAACATTCATTCATTCAGCCATAACTCCACAAAGTAGCACAGAGTTCATAAAAAGCTCATGCAGTTCCACCACATACCCTCGTCTCTGTTTTTCCTCTCTTTCAGTCCACGCTAATGCCATCCAAACGAGCCCTCGCCTTTTAGCCACCGCCGCCCTCTCCGTCTCCCTCTCCTCCGTCAAGTCCGCCGCCACTCAAATCCTCAAGCTTTCCCACGCCCACGGCCTCCCCTCCAGAGATGTCTCTGCTCTGAACGACTGTTTGGAAGAATTGAGTGATTCTGTCGACTCCCTTGCCGCCTCCATTTCCGAGATGCCGAAGCTCCGTGGCCACGATTTCGATCTTGTTATGAGCAATGTTCAGACTTGGGTCAGCGCCGCCCTCACCGATGAGACCACTTGTAGCGAAGGGTTTCAGGGGAAGGTGGTGAACGGCGGCGTTAAGGCTACGGTTAGGACTAGGATTGTCAACATTGCACAGTTGACTAGTAATGCTCTCTCTTTGATCAACCGGATTGCCGATCTTTATTAG

mRNA sequence

ATGGGTGTCTCAAATTCCAAGAAAATTTTGATTCTTTTCCAATTAATTCTCCAATTAACATTCATTCATTCAGCCATAACTCCACAAAGTAGCACAGAGTTCATAAAAAGCTCATGCAGTTCCACCACATACCCTCGTCTCTGTTTTTCCTCTCTTTCAGTCCACGCTAATGCCATCCAAACGAGCCCTCGCCTTTTAGCCACCGCCGCCCTCTCCGTCTCCCTCTCCTCCGTCAAGTCCGCCGCCACTCAAATCCTCAAGCTTTCCCACGCCCACGGCCTCCCCTCCAGAGATGTCTCTGCTCTGAACGACTGTTTGGAAGAATTGAGTGATTCTGTCGACTCCCTTGCCGCCTCCATTTCCGAGATGCCGAAGCTCCGTGGCCACGATTTCGATCTTGTTATGAGCAATGTTCAGACTTGGGTCAGCGCCGCCCTCACCGATGAGACCACTTGTAGCGAAGGGTTTCAGGGGAAGGTGGTGAACGGCGGCGTTAAGGCTACGGTTAGGACTAGGATTGTCAACATTGCACAGTTGACTAGTAATGCTCTCTCTTTGATCAACCGGATTGCCGATCTTTATTAG

Coding sequence (CDS)

ATGGGTGTCTCAAATTCCAAGAAAATTTTGATTCTTTTCCAATTAATTCTCCAATTAACATTCATTCATTCAGCCATAACTCCACAAAGTAGCACAGAGTTCATAAAAAGCTCATGCAGTTCCACCACATACCCTCGTCTCTGTTTTTCCTCTCTTTCAGTCCACGCTAATGCCATCCAAACGAGCCCTCGCCTTTTAGCCACCGCCGCCCTCTCCGTCTCCCTCTCCTCCGTCAAGTCCGCCGCCACTCAAATCCTCAAGCTTTCCCACGCCCACGGCCTCCCCTCCAGAGATGTCTCTGCTCTGAACGACTGTTTGGAAGAATTGAGTGATTCTGTCGACTCCCTTGCCGCCTCCATTTCCGAGATGCCGAAGCTCCGTGGCCACGATTTCGATCTTGTTATGAGCAATGTTCAGACTTGGGTCAGCGCCGCCCTCACCGATGAGACCACTTGTAGCGAAGGGTTTCAGGGGAAGGTGGTGAACGGCGGCGTTAAGGCTACGGTTAGGACTAGGATTGTCAACATTGCACAGTTGACTAGTAATGCTCTCTCTTTGATCAACCGGATTGCCGATCTTTATTAG

Protein sequence

MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTSNALSLINRIADLY

Homology

[Close]

BLAST of Bhi01G000900 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 4.2e-43
Identity = 93/186 (50.00%), Postives = 139/186 (74.73%), Query Frame = 0


Query: 9   ILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLAT 68
           ++I+  L L  T   +  T  ++TEF+KSSC+ TTYPRLCFSSLS HA+ IQTSP+L+A 
Sbjct: 18  LIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAH 77

Query: 69  AALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRG 128
           AAL+++L+S K  +  +++LS++  L  ++VSA+ DC+EEL D+++ L  SI EM +L G
Sbjct: 78  AALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSG 137

Query: 129 HDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTSNALSLIN 188
            ++++ +S++QTWVSAALTD  TC++GF+G+ ++G VK  VR RI+ IA LTSNAL+LIN
Sbjct: 138 SNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALIN 197

Query: 189 RIADLY 195
             A ++
Sbjct: 198 HFASIH 202

[Close]

BLAST of Bhi01G000900 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 154.8 bits (390), Expect = 6.9e-38
Identity = 94/192 (48.96%), Postives = 126/192 (65.62%), Query Frame = 0


Query: 11  ILFQLILQLTFIHSAITPQSSTE---FIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLA 70
           I + L L+LT   +A    +S +   FI+SSC +TTYP LC  SLSV+AN IQTSP+ LA
Sbjct: 12  ISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLA 71

Query: 71  TAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLR 130
             A++V+LS  +S    + +L+   GL  R+V A+ DC+EE++D+VD L  S+ E+ KL 
Sbjct: 72  ETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQEL-KLC 131

Query: 131 G-----HDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTSN 190
           G       F   MSN QTW SAALTDE TCS+GF G+V++G +K +VR RI+N+   TSN
Sbjct: 132 GSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSN 191

Query: 191 ALSLINRIADLY 195
           ALSLIN  A  Y
Sbjct: 192 ALSLINAFAKTY 202

[Close]

BLAST of Bhi01G000900 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 3.8e-36
Identity = 92/195 (47.18%), Postives = 126/195 (64.62%), Query Frame = 0


Query: 5   NSKKILILFQLILQLTFIHSA--ITPQSS--TEFIKSSCSSTTYPRLCFSSLSVHANAIQ 64
           N +  LILF L L    I  A  +  +SS  TEFIK+SC +T+YP  CF SLS +A+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 65  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 124
             PR LA  AL+VS++  KSA T + +++   G+  R   A+ DCLEE+ D+VD L+ S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 125 SEMPKLR----GHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNI 184
            E+  L     G DF   +SNV+TW SAALTDET C +GF GK + G +K+ +RT IV++
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 185 AQLTSNALSLINRIA 192
           A+ TSNAL+LIN  A
Sbjct: 184 AEETSNALALINDFA 198

[Close]

BLAST of Bhi01G000900 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 4.2e-35
Identity = 82/175 (46.86%), Postives = 118/175 (67.43%), Query Frame = 0


Query: 24  SAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSAAT 83
           +AIT + +  FI++SC +TTYP +C +SL+ +AN+IQTSPR LA  AL+V+++  +S   
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 84  QILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASISEMP---KLRGHD-FDLVMSNVQ 143
            + +L     L  R++ A+ DC+EE+ D+VD L  SI E+      +G D F   MSN Q
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQ 147

Query: 144 TWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTSNALSLINRIADLY 195
           TW SAALT+  TCS+GF G+V++G VK +VR RI+N+ + TSNAL+LIN  A  Y
Sbjct: 148 TWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAKKY 202

[Close]

BLAST of Bhi01G000900 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 136.0 bits (341), Expect = 3.3e-32
Identity = 78/171 (45.61%), Postives = 118/171 (69.01%), Query Frame = 0


Query: 24  SAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSAAT 83
           S  + Q++ ++IK+SC+ T Y  +C++SLS +A+ I+++P+ LA  AL+++LSS KSA+ 
Sbjct: 137 STYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASK 196

Query: 84  QILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGHD---FDLVMSNVQT 143
            +  +SH  GL   +V A+ DC+EE+ DSV SL  SI E+  +   D   F++VMS+V+T
Sbjct: 197 FVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVET 256

Query: 144 WVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTSNALSLINRIA 192
           WVSAALT++ TC +GF   +V   VK  VR  +V +A+LTSNAL+LIN  A
Sbjct: 257 WVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of Bhi01G000900 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.2e-36
Identity = 87/189 (46.03%), Postives = 129/189 (68.25%), Query Frame = 0


Query: 6   SKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRL 65
           SK  L+   L++    I +A +   + +FIK+SC+ TTYP +C  SLS +A  IQ +P+ 
Sbjct: 4   SKSTLVFSALLV----ILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQE 63

Query: 66  LATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASISEMPK 125
           LA+ AL VSL+  + A T + +L+   GL +R  +A++DCLEE+ DS+D ++ S  EM  
Sbjct: 64  LASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKN 123

Query: 126 L---RGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTSN 185
           L   +G+DF   MSNV+TWVSAALTDETTC +GF GK ++G +K +VR ++V +A++TSN
Sbjct: 124 LSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSN 183

Query: 186 ALSLINRIA 192
           AL+L+N  A
Sbjct: 184 ALALVNNFA 188

[Close]

BLAST of Bhi01G000900 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 5.3e-35
Identity = 92/195 (47.18%), Postives = 126/195 (64.62%), Query Frame = 0


Query: 5   NSKKILILFQLILQLTFIHSA--ITPQSS--TEFIKSSCSSTTYPRLCFSSLSVHANAIQ 64
           N +  LILF L L    I  A  +  +SS  TEFIK+SC +T+YP  CF SLS +A+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 65  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 124
             PR LA  AL+VS++  KSA T + +++   G+  R   A+ DCLEE+ D+VD L+ S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 125 SEMPKLR----GHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNI 184
            E+  L     G DF   +SNV+TW SAALTDET C +GF GK + G +K+ +RT IV++
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 185 AQLTSNALSLINRIA 192
           A+ TSNAL+LIN  A
Sbjct: 184 AEETSNALALINDFA 198

[Close]

BLAST of Bhi01G000900 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 5.9e-34
Identity = 82/175 (46.86%), Postives = 118/175 (67.43%), Query Frame = 0


Query: 24  SAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSAAT 83
           +AIT + +  FI++SC +TTYP +C +SL+ +AN+IQTSPR LA  AL+V+++  +S   
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 84  QILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASISEMP---KLRGHD-FDLVMSNVQ 143
            + +L     L  R++ A+ DC+EE+ D+VD L  SI E+      +G D F   MSN Q
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQ 147

Query: 144 TWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTSNALSLINRIADLY 195
           TW SAALT+  TCS+GF G+V++G VK +VR RI+N+ + TSNAL+LIN  A  Y
Sbjct: 148 TWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAKKY 202

[Close]

BLAST of Bhi01G000900 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 4.7e-31
Identity = 78/171 (45.61%), Postives = 118/171 (69.01%), Query Frame = 0


Query: 24  SAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSAAT 83
           S  + Q++ ++IK+SC+ T Y  +C++SLS +A+ I+++P+ LA  AL+++LSS KSA+ 
Sbjct: 137 STYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASK 196

Query: 84  QILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRGHD---FDLVMSNVQT 143
            +  +SH  GL   +V A+ DC+EE+ DSV SL  SI E+  +   D   F++VMS+V+T
Sbjct: 197 FVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVET 256

Query: 144 WVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTSNALSLINRIA 192
           WVSAALT++ TC +GF   +V   VK  VR  +V +A+LTSNAL+LIN  A
Sbjct: 257 WVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of Bhi01G000900 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-28
Identity = 82/194 (42.27%), Postives = 119/194 (61.34%), Query Frame = 0


Query: 8   KILILFQLILQLTFIH-SAITP--QSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ-TSP 67
           K  I   ++L +T +  S+ TP    S +FI SSC +T YP LC  +LS +A  I+  + 
Sbjct: 4   KNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNND 63

Query: 68  RLLATAALSVSLSSVKSAATQILKLS-HAHGLPSRDVSALNDCLEELSDSVDSLAASISE 127
           + LA  AL +SL+  KS    + KL+        R+  A+ DC+E L +SVD LA S+ E
Sbjct: 64  QDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKE 123

Query: 128 MPK----LRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQ 187
           + +    +   DF   MSNVQTWVSAALTDETTC +GF  + + G VK  +R ++V++AQ
Sbjct: 124 LARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQ 183

Query: 188 LTSNALSLINRIAD 193
           +TSNAL+L+N+ A+
Sbjct: 184 VTSNALALVNQFAE 197

[Close]

BLAST of Bhi01G000900 vs. NCBI nr
Match: XP_038882403.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 358.6 bits (919), Expect = 3.4e-95
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180
           SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180

Query: 181 SNALSLINRIADLY 195
           SNALSLINRIADLY
Sbjct: 181 SNALSLINRIADLY 194

[Close]

BLAST of Bhi01G000900 vs. NCBI nr
Match: XP_004148138.3 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 337.0 bits (863), Expect = 1.1e-88
Identity = 182/194 (93.81%), Postives = 187/194 (96.39%), Query Frame = 0


Query: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+SNSKK LILFQLILQLT IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKS ATQILKLSH+HGLPSRDVSALNDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALNDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180
           SEMPKLRG +FDL MSNVQTWVSAALTDETTCSEGFQGK VNGGVKA VRT+IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLT 180

Query: 181 SNALSLINRIADLY 195
           SNALSLINRIADL+
Sbjct: 181 SNALSLINRIADLH 194

[Close]

BLAST of Bhi01G000900 vs. NCBI nr
Match: XP_011652768.2 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 332.0 bits (850), Expect = 3.4e-87
Identity = 179/194 (92.27%), Postives = 185/194 (95.36%), Query Frame = 0


Query: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+SNSKK LILFQLILQLT IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKS ATQILKLSH+HGLPSRDVSAL+DCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180
           SEMPKLRG +FDL MSNVQTWVSAALTDETTCSEGFQGK VNGGVK  VRT+IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIADLY 195
           SNALSLINRI DL+
Sbjct: 181 SNALSLINRIGDLH 194

[Close]

BLAST of Bhi01G000900 vs. NCBI nr
Match: XP_022922872.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 317.0 bits (811), Expect = 1.1e-82
Identity = 169/194 (87.11%), Postives = 182/194 (93.81%), Query Frame = 0


Query: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+S  KK LI  Q+ILQLTFIHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLAT ALSV+LSSVKSAATQILKLSHAHGLP RDVSALNDCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180
           SEMPKLRGHDFDLVMS+VQTWVSAALTDETTCSEGFQGK V GGVKA VR RIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIADLY 195
           SNALSLINRIA+L+
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of Bhi01G000900 vs. NCBI nr
Match: XP_023552889.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.8 bits (808), Expect = 2.5e-82
Identity = 169/194 (87.11%), Postives = 181/194 (93.30%), Query Frame = 0


Query: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGVS  KK LI  Q+ILQLTFIHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGVSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLAT ALSV+LSSVKSAATQILKLSHAHGLP RDVSALNDCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180
           SEMPKLRGHD DLVMS+VQTWVSAALTDETTCSEGFQGK V GGVKA VR RIVNIAQLT
Sbjct: 121 SEMPKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIADLY 195
           SNALSLINRIA+L+
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of Bhi01G000900 vs. ExPASy TrEMBL
Match: A0A0A0LB27 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.3e-88
Identity = 180/194 (92.78%), Postives = 186/194 (95.88%), Query Frame = 0


Query: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+SNSKK LILFQLILQLT IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKS ATQILKLSH+HGLPSRDVSAL+DCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180
           SEMPKLRG +FDL MSNVQTWVSAALTDETTCSEGFQGK VNGGVK  VRT+IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIADLY 195
           SNALSLINRIADL+
Sbjct: 181 SNALSLINRIADLH 194

[Close]

BLAST of Bhi01G000900 vs. ExPASy TrEMBL
Match: A0A0A0L8D1 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.8e-87
Identity = 178/193 (92.23%), Postives = 184/193 (95.34%), Query Frame = 0


Query: 2   GVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 61
           G+SNSKK LILFQLILQLT IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASIS 121
           SPRLLATAALSVSLSSVKS ATQILKLSH+HGLPSRDVSAL+DCLEELSDSVDSLAASIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTS 181
           EMPKLRG +FDL MSNVQTWVSAALTDETTCSEGFQGK VNGGVK  VRT+IVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINRIADLY 195
           NALSLINRI DL+
Sbjct: 224 NALSLINRIGDLH 236

[Close]

BLAST of Bhi01G000900 vs. ExPASy TrEMBL
Match: A0A6J1E4J6 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430716 PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 5.4e-83
Identity = 169/194 (87.11%), Postives = 182/194 (93.81%), Query Frame = 0


Query: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+S  KK LI  Q+ILQLTFIHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLAT ALSV+LSSVKSAATQILKLSHAHGLP RDVSALNDCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180
           SEMPKLRGHDFDLVMS+VQTWVSAALTDETTCSEGFQGK V GGVKA VR RIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIADLY 195
           SNALSLINRIA+L+
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of Bhi01G000900 vs. ExPASy TrEMBL
Match: A0A6J1JDH5 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483501 PE=4 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.6e-82
Identity = 168/194 (86.60%), Postives = 182/194 (93.81%), Query Frame = 0


Query: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+S  KK LI  Q+ILQLT IHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLAT ALSV+LSSVKSAATQILKLSHAHGLP+RDVSALNDCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180
           SEMPKLRGHDFDLVMS+VQTWVSAALTDETTCSEGFQGK V GGVKA VR RIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIADLY 195
           SNALSLINRIA+L+
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of Bhi01G000900 vs. ExPASy TrEMBL
Match: A0A6J1CHT4 (21 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111011621 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 3.4e-77
Identity = 159/186 (85.48%), Postives = 176/186 (94.62%), Query Frame = 0


Query: 9   ILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLAT 68
           +L++F L+L+L+  H+AITP SSTEFIKSSCSSTTYPRLCFSSLSVHA++IQTSPRLLAT
Sbjct: 13  VLVVFPLVLKLSGSHAAITPMSSTEFIKSSCSSTTYPRLCFSSLSVHASSIQTSPRLLAT 72

Query: 69  AALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASISEMPKLRG 128
           AALSVSLSSVKSAA QILKLSHAHG+PSRDVSAL DCLEELSDSVDSL++SI EM KLRG
Sbjct: 73  AALSVSLSSVKSAAAQILKLSHAHGVPSRDVSALKDCLEELSDSVDSLSSSIPEMGKLRG 132

Query: 129 HDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLTSNALSLIN 188
            DF+LVMSN+QTWVSAALTDETTCS+GFQGK VNGGVKATVR+RIVNIAQLTSNALSLIN
Sbjct: 133 PDFELVMSNIQTWVSAALTDETTCSDGFQGKAVNGGVKATVRSRIVNIAQLTSNALSLIN 192

Query: 189 RIADLY 195
           RIADL+
Sbjct: 193 RIADLH 198

The following BLAST results are available for this feature:

BLAST of Bhi01G000900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	4.2e-43	50.00	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	6.9e-38	48.96	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	3.8e-36	47.18	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	4.2e-35	46.86	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	3.3e-32	45.61	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Bhi01G000900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	2.2e-36	46.03	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	5.3e-35	47.18	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9STY5	5.9e-34	46.86	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI74	4.7e-31	45.61	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9SI72	2.2e-28	42.27	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]

BLAST of Bhi01G000900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882403.1	3.4e-95	100.00	21 kDa protein-like [Benincasa hispida]	[more]
XP_004148138.3	1.1e-88	93.81	21 kDa protein [Cucumis sativus]	[more]
XP_011652768.2	3.4e-87	92.27	21 kDa protein [Cucumis sativus]	[more]
XP_022922872.1	1.1e-82	87.11	21 kDa protein-like [Cucurbita moschata]	[more]
XP_023552889.1	2.5e-82	87.11	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Bhi01G000900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB27	4.3e-88	92.78	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176245 PE=4 S...	[more]
A0A0A0L8D1	4.8e-87	92.23	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G175740 PE=4 S...	[more]
A0A6J1E4J6	5.4e-83	87.11	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430716 PE=4 SV=1	[more]
A0A6J1JDH5	1.6e-82	86.60	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483501 PE=4 SV=1	[more]
A0A6J1CHT4	3.4e-77	85.48	21 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111011621 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..186 e-value: 5.3E-49 score: 178.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..186 e-value: 1.2E-36 score: 126.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 8..189 e-value: 1.3E-35 score: 120.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 29..191 e-value: 3.8E-39 score: 136.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..190
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 25..190
None	No IPR available	PANTHER	PTHR31080:SF161	21 KDA PROTEIN-LIKE	coord: 25..190
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 37..191 e-value: 7.1606E-43 score: 139.11

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M000900	Bhi01M000900	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0009827	plant-type cell wall modification
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004222	metalloendopeptidase activity