Bhi01G000777 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000777
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPlant protein of unknown function (DUF247)
Locationchr1: 20429372 .. 20434857 (-)
RNA-Seq ExpressionBhi01G000777
SyntenyBhi01G000777
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATATTTAGGCCAAAAAAGTATGGGACAATGTGTGAGACAAAAGCTTGAGATAAGAAATTCCTTAGGGTTGTGTGCCACAAATTAATTAATGATAATCTCTAAAGGCTAGATTGTAACATTTTAAACTAGGATTTCAAATTAGTACTTTTAACTTTATGTACTGATCTATTGATAGAAATGTTGAGTTCTATTTTATCCAAATTAGTGGAAACATTAAAATTTCACTGAGAAAAAAAATTAAATTAATAAAAACACATTTTACATTTTTAAAAAAAAAAGGTTCTATTTTTTTTTTCTTTTGTTATTAATATTTGATCTACATTAGAGTTTTTCTTTTTTTATATTAGATTTGGCCAATTTTTCGAACAATAAAATAGAAATATTGATCGACTCTATGTTGATGTTAGACTCATGAACATGTAGAAATATTGACATGTTATGGAAATTATAATTTTTTAAAATATCTTTAAATAATAATAAGCATGGATTGAAGTTTTAAAAGCCAAGCAAGATGGAGTATTTTCACTTTTGGAACTGTACCACCAATTCTTGAATTATATTTGAAGCAATGAATTGTTAATTTTGTTGCTCTTTAGCAAAAGTTTAATATGCCTTTGGTCTTTGCTTCTAAAATGGAATGAATTTGCCTTAGTCTGTGATCTTCATGTGTTTCACCTTAATTTCTCTTTAGTTTCTCCTTGGCTAATTCCCCAAGTCACCCCTTGATTCTATTTTTGCCTCCCTAAACCTTACATATTATTATATATCATCCTCCTACATGCTTAATAATACATTACATAACCACTTATTCTTTTTCCTTTCTTTCTTTAATTAATTTTTCACTCCATCTTCTTTCAAGGCATCACGAGATTTTCAGTTCTCTTAAGGTATGTTGAGTCAATAATTTTTTTTTTTTTTTTTTTTTTTGCAAATATTGTGTAAAAATTGTCTATAAGCTAATGATGATTATTTTTATAAGTAGAAGAAGATTAATACTAAGGAAGAACCTATTTTTTTTATTGACATTTGATCACTTGATTAGTTTAAATATTATTCTCCTCGGTAAGACTCATTGTTCTTTTAATATTTTACTTATTTGGCAATAAATAAACAGTTGAAATCCCAAAGTTTAAAGGTTTATATGATTTGGTTTATTTTGATTCATATATTTTTAATTTCTATTCATTTTAGTGTTCGTACTTTCAAAAAGTCTATCTTAATCTTTGTGTTTACATAATGATCATTTTAATTTGTGTTTGCAAAATTCTATTTTAAGTTCTTTCTATAGCTTAGACTCTTTAATACAAAGTTGTTACATTTCCAAAAAAAGAAAAAAGAATTATTGAGTGTAGTGTAGTGTTGAAATGATGATTGAAAACAATGACAAAAAGTGAAGGAGAACTAAATGGAATGTTTCAAAAGGACAAGGACCAAGTGGATACTTTCAAAAGTGTGTAAATTAAAATGGGTGAAAACTGAAGGTAGAATTACGTGGAAGGTTTTTGAAATTTTAGATACTAAAGTAAATCAAAGTATAAAGTATTAAGACCAAAATAGTAATTAAACCCAATTTAATTAACTTAACGAAGTCTTAGAGAAAGTAAAATGACATTTCTTTTTTAAAAAAATAAAATTTAAACCTAATTTAATTAACTTAACGAAGTCTTAGAGAAAGTAAAACGAATTTCTTTTTTTAAAAATAAAGTTATGTTAGATTGTATAATATATATCCGAATTTATTGTCAATTGAAATGTAGGGTGAAACCTTCCATTTTTACTTTCCAGTATTCGTTCCATATTTTGTATGTATTGGGCTCTCTCTTTATGTCTATTGAGTTGGTTTTCTTATTTGTTTGTATATAGAAATTCGATTTTTTCGACCCTTTGTTTGAGAATAAATTATTTGACTGATTCAACTATGTTCTAGTACACTTAGTTCATAAGTTATCTGCAAAATCTTATAAAATTAATATTAATTATTTGTTGCAAATCAAACAAAGACACTAGCTGAACCATACAAAATATTTTTAGACTTTGTATTGTGTCAAAAAATACTCTTAGAATTTTATATTTCAAAAATACCTTTTTGTTTTCTATAAAAGTTGACAAATACTTTTACCATTAATTTTGGATGGAAATTTTTATTTTAAAGATATCATGAACTTCCAAAAGTCTAAAAAAATACTCTTAATCTTTAAAAAAAAAATCTAAAAATACTTTTAATTGTTAGTATTTGGAAACATGCGGTTATTATTTGTTTAAAAGATATCCCTAAATTTTTAAAAGTTGCATTCTTACTCTTAACTTACAAAAAGAAAAGTTTAAAAAATGCTTCTGCCATCAGTATGATGATCAATTTAGTTGATGTTTTCCGGAATTTGAAGAGAACCAATTTTAAAACAGACTATATAGATATTTTTGTTCCATATATACTCTCATTTTTCTTCAATTTGTCCAATTCTTGTACCAATTTTCTCACAATAAAATATAAACTAAAACTCTATGTTAGCATAAAATCTAGGTATTTTTACATCGAGGTACTTGTTTGTATATTAATAGTAAGGATGTTTTTTAACATTCTTTTTTAAGTTTAAGGGTAATTTTTAAACGTTTGAAAGTTTAAGACATTTTTAAAATAAAATACTGATGATTTACATCCAAATTATAAGAGTTTTTTTTTTTTTAACTCTTTTCGAAAACTAACAAGTATTACTTTTGAAATTCAAGGTATTTTTGACATCAAGTAGAAGTACAAAGTTAAAGGTCATTTTTATAATTTAACCAAAGATATTTGTCATAAACTTATATTATTCCTCACAAAATTTTATTTATTTAATGTGTAAACCAAAGTTCAAAATGTCGATGTACATGGAAATATTGAAGTCCTAATTTTATGGAAATATCAATATCAATGGAAATTTCGGAAAACTTTATTGAAATTGATGGTAATTGATGGAAATTGTTATAATTAGTTAATGAAGCTTTAATTGTTGCTGAATTAGACTATAATTGACTATTTTTAATAATCATTTCTATAAAATAAGACAACATTTAGATGTATATTATAAAATATTTGTGTAAATTTGATGTGAGAACAAAAATGATGAAAAATAATTTTAAAATATTTGAATATAAAATAATTGTAAATTATATGTTATAAAATAGAGTGGATACAAAAAAAAAAAAATAAAAAATCAGTACATTTTAATTTGAAGTGAAATGATAGAAAAATTCAATGAAAAATATTTGAATGAAAATACATGGTTTCTTGAAAATAAAGATTTTACACTGTAAAAGAGGAAAAACTTTTCTTGAACTAACATACAAAAAATGAAAATTCGAAATTCAGATAAAAATATCAAAATATCATAGAAATTTCGAAATTTCAAGAAATTGGAGAAAATGGAAATTCCTCCTTTACCAAAATTTGAAACCTAACGAAATTTTGAGTAAGGGTTAAACACACTCACTTAAAACATCAAAATCATTGCGGTCTTTTTTTTGGGAAAAAGTTCTGTTTTAAACTAAAATTGAAACTAATATTTAAGAATTTTTAAGATTTTGATACAAAGTTTTCTTCATCTTAATTTCGATAGTTTCAAAAATGTTAGTTTCACTGATGTAATTATCATTAATAAACATTTTAACATAGACTTTTTGGTCGATGCTTTCCCTTTTTGTTTTTATGATTTTTCATACTTAAAATCTCGATCTACGTGCATAGGTTCATTCATTATTATTGTTTTTATTACTATTCATTTTCTTAATGAGACTATTGTTCTTTTCTTATTCTCATAATTATTTTTGATGAATTAGATCCTTAAAGAACAATATTTTACCAATTACCTTACCTCTGTCCCTGAAGTAAAGAGCCTCTGTATAATCTCTGGCCGTCGCCGGAAAGATGGAATCATCAAAGCCATTTTCTCATTCGATTGATATTTCGGCGATTGCACAAGGAAGCTCTCAAGAAGAATCTCTCCTATCTTCCGTTGAAGGAAAATTGGAAGCCTTCTGTTCATCCATTACCATCTTCAGAGCTCCAAACGATATCAGTATCGAAGACAAAAACGTCTTCGTCCCCGCCAAAGTCTCAATCGGCCCTTTCCACCATGGCGCTCCACATCTCGAACCCATGGAAAATCTCAAGTGGCGCTACTTGTCCACTTTCTTGAAGCACAATCCGTCTCTCACTTTAGACGATCTTATTGAACTCGTTGTAAAATCAGAGAGCCGATTGAGAAAATGCTATGAGGGAGAGTTTTATGATTTGGATAGTGATAAGTTTTCGCAGATGATGTTGCTTGATTGCTGCTTCATTCTCGAGTTGCTTTTGCGATACTCGAAAAAGAGGTTCAGGCGCTGGAATGATCCTGTTTTCAATACTCCTGGTTTGCTTTTCGATTTGAGATGCGATTTGATGTTGCTTGAAAATCAGATTCCATACTTCCTTCTCGATGAAGTTTATGAAAATGTGCAAGATCCGCTCGAGGAAAATATGTCTCTCAATGACCTAACCTTCCGATTCTTCAAAACTATGGTTGCTGGAGATCGGAAATTTGTCTATGACAATTTCATGGTGGAAGCAGATCATCTACTCGAAATGGTGCACTCTTGTTTCCTCTCTACCTATCCTCGAATGGAGACGAACGATAAATCGAAGTCGAGAGAATTACCTAGTGCGTCGAAGCTTAAAACTGCCGGAATCAAATTCAAGAACGCCAGATCTCCAAAGAGCCTATTGGACATCAAATTTCAGAAAGGAGTCCTCGAAATTCCGCCTCTCAGAGTGTACCAGCAGACGGAGGCGATTCTGAGGAATCTCGCCGCGTATGAAATCCGTCAATTCGGAAGCGATCTGCAAGTGAAATCGTATATCAATTTCATGAGCCACCTTCTCCAGTCCGACGAAGACGTGAAGATACTCTGTAGAAGGAAAATCCTGATCGATCTGGAGGACGACGAGGAGCAGATTATTCAGAATCTAAAATGGATGCGCGAGGAGAAGGAGAGCTTATCGGGAACGTACTTTGCTGGCATTGTTCAGAAATTAAACGAGAAGCCGGACCGATGCCTCACACAGTGGCGGGGGCTGAGAAGGAATCCAGTAGCCATCGGCGTCGCCGCCGTTTGGGTGGTGGTTGTGATCTTCGTCGCAGCCTTCTTCTCTGCAATTTCATTACTTCAGCGCCGTTACAAATGAGTCATGGAGTGCACCTTTTAAGTTTTAGCTATACGGTTTTATGATCATATCAAACAACTTTCATTTTGGTGATAAGTCCCTAAATTTACTGAATTTTACGGTTAAGCCCTTCACTATTGCAAAATACATTTTTGAACTATATGGAAAGTTTTCTTTTCTTTTCTTTTCTCTTTTTTTTTTTTATTTAAATACTATTTTAGTGTATGTACTTTTAACTTTTATTTATTTTGGTTCTTCTACGTTTAAAATATTTATTTTAGTCTCTATACTTTTAACTTTCG

mRNA sequence

CTATATTTAGGCCAAAAAAGTATGGGACAATGTGTGAGACAAAAGCTTGAGATAAGAAATTCCTTAGGGTTGTGTGCCACAAATTAATTAATGATAATCTCTAAAGGCTAGATTGTAACATTTTAAACTAGGATTTCAAATTAGTACTTTTAACTTTATGTACTGATCTATTGATAGAAATGTTGAGTTCTATTTTATCCAAATTAGTGGAAACATTAAAATTTCACTGAGAAAAAAAATTAAATTAATAAAAACACATTTTACATTTTTAAAAAAAAAAGGTTCTATTTTTTTTTTCTTTTGTTATTAATATTTGATCTACATTAGAGTTTTTCTTTTTTTATATTAGATTTGGCCAATTTTTCGAACAATAAAATAGAAATATTGATCGACTCTATGTTGATGTTAGACTCATGAACATGTAGAAATATTGACATGTTATGGAAATTATAATTTTTTAAAATATCTTTAAATAATAATAAGCATGGATTGAAGTTTTAAAAGCCAAGCAAGATGGAGTATTTTCACTTTTGGAACTGTACCACCAATTCTTGAATTATATTTGAAGCAATGAATTGTTAATTTTGTTGCTCTTTAGCAAAAGTTTAATATGCCTTTGGTCTTTGCTTCTAAAATGGAATGAATTTGCCTTAGTCTGTGATCTTCATGTGTTTCACCTTAATTTCTCTTTAGTTTCTCCTTGGCTAATTCCCCAAGTCACCCCTTGATTCTATTTTTGCCTCCCTAAACCTTACATATTATTATATATCATCCTCCTACATGCTTAATAATACATTACATAACCACTTATTCTTTTTCCTTTCTTTCTTTAATTAATTTTTCACTCCATCTTCTTTCAAGGCATCACGAGATTTTCAGTTCTCTTAAGATCCTTAAAGAACAATATTTTACCAATTACCTTACCTCTGTCCCTGAAGTAAAGAGCCTCTGTATAATCTCTGGCCGTCGCCGGAAAGATGGAATCATCAAAGCCATTTTCTCATTCGATTGATATTTCGGCGATTGCACAAGGAAGCTCTCAAGAAGAATCTCTCCTATCTTCCGTTGAAGGAAAATTGGAAGCCTTCTGTTCATCCATTACCATCTTCAGAGCTCCAAACGATATCAGTATCGAAGACAAAAACGTCTTCGTCCCCGCCAAAGTCTCAATCGGCCCTTTCCACCATGGCGCTCCACATCTCGAACCCATGGAAAATCTCAAGTGGCGCTACTTGTCCACTTTCTTGAAGCACAATCCGTCTCTCACTTTAGACGATCTTATTGAACTCGTTGTAAAATCAGAGAGCCGATTGAGAAAATGCTATGAGGGAGAGTTTTATGATTTGGATAGTGATAAGTTTTCGCAGATGATGTTGCTTGATTGCTGCTTCATTCTCGAGTTGCTTTTGCGATACTCGAAAAAGAGGTTCAGGCGCTGGAATGATCCTGTTTTCAATACTCCTGGTTTGCTTTTCGATTTGAGATGCGATTTGATGTTGCTTGAAAATCAGATTCCATACTTCCTTCTCGATGAAGTTTATGAAAATGTGCAAGATCCGCTCGAGGAAAATATGTCTCTCAATGACCTAACCTTCCGATTCTTCAAAACTATGGTTGCTGGAGATCGGAAATTTGTCTATGACAATTTCATGGTGGAAGCAGATCATCTACTCGAAATGGTGCACTCTTGTTTCCTCTCTACCTATCCTCGAATGGAGACGAACGATAAATCGAAGTCGAGAGAATTACCTAGTGCGTCGAAGCTTAAAACTGCCGGAATCAAATTCAAGAACGCCAGATCTCCAAAGAGCCTATTGGACATCAAATTTCAGAAAGGAGTCCTCGAAATTCCGCCTCTCAGAGTGTACCAGCAGACGGAGGCGATTCTGAGGAATCTCGCCGCGTATGAAATCCGTCAATTCGGAAGCGATCTGCAAGTGAAATCGTATATCAATTTCATGAGCCACCTTCTCCAGTCCGACGAAGACGTGAAGATACTCTGTAGAAGGAAAATCCTGATCGATCTGGAGGACGACGAGGAGCAGATTATTCAGAATCTAAAATGGATGCGCGAGGAGAAGGAGAGCTTATCGGGAACGTACTTTGCTGGCATTGTTCAGAAATTAAACGAGAAGCCGGACCGATGCCTCACACAGTGGCGGGGGCTGAGAAGGAATCCAGTAGCCATCGGCGTCGCCGCCGTTTGGGTGGTGGTTGTGATCTTCGTCGCAGCCTTCTTCTCTGCAATTTCATTACTTCAGCGCCGTTACAAATGAGTCATGGAGTGCACCTTTTAAGTTTTAGCTATACGGTTTTATGATCATATCAAACAACTTTCATTTTGGTGATAAGTCCCTAAATTTACTGAATTTTACGGTTAAGCCCTTCACTATTGCAAAATACATTTTTGAACTATATGGAAAGTTTTCTTTTCTTTTCTTTTCTCTTTTTTTTTTTTATTTAAATACTATTTTAGTGTATGTACTTTTAACTTTTATTTATTTTGGTTCTTCTACGTTTAAAATATTTATTTTAGTCTCTATACTTTTAACTTTCG

Coding sequence (CDS)

ATGGAATCATCAAAGCCATTTTCTCATTCGATTGATATTTCGGCGATTGCACAAGGAAGCTCTCAAGAAGAATCTCTCCTATCTTCCGTTGAAGGAAAATTGGAAGCCTTCTGTTCATCCATTACCATCTTCAGAGCTCCAAACGATATCAGTATCGAAGACAAAAACGTCTTCGTCCCCGCCAAAGTCTCAATCGGCCCTTTCCACCATGGCGCTCCACATCTCGAACCCATGGAAAATCTCAAGTGGCGCTACTTGTCCACTTTCTTGAAGCACAATCCGTCTCTCACTTTAGACGATCTTATTGAACTCGTTGTAAAATCAGAGAGCCGATTGAGAAAATGCTATGAGGGAGAGTTTTATGATTTGGATAGTGATAAGTTTTCGCAGATGATGTTGCTTGATTGCTGCTTCATTCTCGAGTTGCTTTTGCGATACTCGAAAAAGAGGTTCAGGCGCTGGAATGATCCTGTTTTCAATACTCCTGGTTTGCTTTTCGATTTGAGATGCGATTTGATGTTGCTTGAAAATCAGATTCCATACTTCCTTCTCGATGAAGTTTATGAAAATGTGCAAGATCCGCTCGAGGAAAATATGTCTCTCAATGACCTAACCTTCCGATTCTTCAAAACTATGGTTGCTGGAGATCGGAAATTTGTCTATGACAATTTCATGGTGGAAGCAGATCATCTACTCGAAATGGTGCACTCTTGTTTCCTCTCTACCTATCCTCGAATGGAGACGAACGATAAATCGAAGTCGAGAGAATTACCTAGTGCGTCGAAGCTTAAAACTGCCGGAATCAAATTCAAGAACGCCAGATCTCCAAAGAGCCTATTGGACATCAAATTTCAGAAAGGAGTCCTCGAAATTCCGCCTCTCAGAGTGTACCAGCAGACGGAGGCGATTCTGAGGAATCTCGCCGCGTATGAAATCCGTCAATTCGGAAGCGATCTGCAAGTGAAATCGTATATCAATTTCATGAGCCACCTTCTCCAGTCCGACGAAGACGTGAAGATACTCTGTAGAAGGAAAATCCTGATCGATCTGGAGGACGACGAGGAGCAGATTATTCAGAATCTAAAATGGATGCGCGAGGAGAAGGAGAGCTTATCGGGAACGTACTTTGCTGGCATTGTTCAGAAATTAAACGAGAAGCCGGACCGATGCCTCACACAGTGGCGGGGGCTGAGAAGGAATCCAGTAGCCATCGGCGTCGCCGCCGTTTGGGTGGTGGTTGTGATCTTCGTCGCAGCCTTCTTCTCTGCAATTTCATTACTTCAGCGCCGTTACAAATGA

Protein sequence

MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVPAKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEFYDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIPYFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFLSTYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQTEAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQNLKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAFFSAISLLQRRYK
Homology
BLAST of Bhi01G000777 vs. TAIR 10
Match: AT5G22540.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 172.9 bits (437), Expect = 5.4e-43
Identity = 114/356 (32.02%), Postives = 176/356 (49.44%), Query Frame = 0

Query: 43  IFRAPNDISIEDKNVFVPAKVSIGPFHHGAPHLEPMENLKWRYLSTFL--KHNPSLTLDD 102
           I R P  ++  +   + P  VSIGP+HHG  HL+  +  K R+L  F+           +
Sbjct: 33  IVRIPQSLARINLKAYEPKIVSIGPYHHGKEHLKMTQQHKRRFLKFFVAKMEEKGFVPQE 92

Query: 103 LIELVVKSESRLRKCYEGEFYDLDSDKFSQMMLLDCCFILELLLRYS-KKRFRRWNDPVF 162
           L++ V   E  +R  Y  E   LDS+   QMM+LD CFIL L    S K  +   +DP+F
Sbjct: 93  LVKAVSSLEGVIRGSY-SEDLGLDSENLVQMMVLDGCFILTLFFVVSGKVEYTNLDDPIF 152

Query: 163 NTPGLLFDLRCDLMLLENQIPYFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKF 222
             P +L  +R DL+LLENQ+PY LL  ++E     L     LN++ F FF   +     F
Sbjct: 153 RMPWILPSIRADLLLLENQVPYVLLQTLFET--SKLVTCSGLNEIAFEFFNYSLQKPETF 212

Query: 223 VYDNFMVEADHLLEMVHSCFLSTYPRMETNDKSKSRE---------LPSASKLKTAGIKF 282
              ++ +EA HLL+++   F+    +    D S             + SA KL   GIKF
Sbjct: 213 WEKHYGLEAKHLLDLIRKTFVPVPSQRRIKDHSSKSSFNDHEYLGFVLSAKKLHLRGIKF 272

Query: 283 KNARSPKSLLDIKFQKGVLEIPPLRVYQQTEAILRNLAAYEIRQFGSDLQVKSYINFMSH 342
           K  ++  S+LDI +  GVL IPP+ +   T +I  N  A+E     S   + SY+ FM+ 
Sbjct: 273 KPRKNTDSILDISYSNGVLHIPPVVMDDFTASIFLNCVAFEQLYADSSNHITSYVAFMAC 332

Query: 343 LLQSDEDVKILCRRKILIDLEDDEEQIIQNLKWM-REEKESLSGTYFAGIVQKLNE 386
           L+  + D   L  R+IL +    E+++ +  K + ++    L  +Y A + + +NE
Sbjct: 333 LINEESDASFLSERRILENYFGTEDEVSRFYKRIGKDIALDLEKSYLAKVFEGVNE 385

BLAST of Bhi01G000777 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 172.6 bits (436), Expect = 7.1e-43
Identity = 119/426 (27.93%), Postives = 221/426 (51.88%), Query Frame = 0

Query: 9   HSIDISAIAQGSSQEESLLSSVEGKLEAFCSSIT----IFRAPNDISIEDKNVFVPAKVS 68
           H +  +      ++ ++L+ S++ KL AF SS++    I++ PN +   + + + P  VS
Sbjct: 258 HRLCFAYERMNQNEGDALVDSIKAKL-AFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVS 317

Query: 69  IGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEFYDLD 128
            GP H G   L+ ME+ K+RYL +F+    S +L+DL+ L    E   R CY  E   L 
Sbjct: 318 FGPLHRGKEELQAMEDQKYRYLLSFIPRTNS-SLEDLVRLARTWEQNARSCY-AEDVKLH 377

Query: 129 SDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIPYFLL 188
           SD+F +M+++D  F++ELLLR    R R  ND +F    ++ D+  D++L+ENQ+P+F++
Sbjct: 378 SDEFVEMLVVDGSFLVELLLRSHYPRLRGENDRIFGNSMMITDVCRDMILIENQLPFFVV 437

Query: 189 DEVYENVQDPLEENM-SLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFLSTY 248
            E++  + +  ++   S+  L  R F   ++   +   + F+ E +H ++++ SC+L  +
Sbjct: 438 KEIFLLLLNYYQQGTPSIIQLAQRHFSYFLS---RIDDEKFITEPEHFVDLLRSCYLPQF 497

Query: 249 PRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQTEAI 308
           P        K    P A++L TAG++FK A +   LLDI F  GVL+IP + V   TE++
Sbjct: 498 PIKLEYTTVKVDNAPEATELHTAGVRFKPAETSSCLLDISFADGVLKIPTIVVDDLTESL 557

Query: 309 LRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQNLKW 368
            +N+  +E  +  S+     YI  +   ++S  D  +L    I+++   +   +      
Sbjct: 558 YKNIIGFEQCRC-SNKNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYLGNSVDVSNLFNS 617

Query: 369 MREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRR-----NPVAIGVAAVWVVVVIFVA 425
           + +E       YF+ + + L    +    +W+ + R     NP A  VA+V+  +++ + 
Sbjct: 618 ISKEVIYDRRFYFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWA--VASVFAALLLLLL 674

BLAST of Bhi01G000777 vs. TAIR 10
Match: AT3G50140.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 166.8 bits (421), Expect = 3.9e-41
Identity = 129/421 (30.64%), Postives = 201/421 (47.74%), Query Frame = 0

Query: 41  ITIFRAPNDISIEDKNVFVPAKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDD 100
           I I+R P  +   DKN + P  VS+GP+HHG  HL PM+  KWR ++  +K      ++ 
Sbjct: 113 ICIYRVPLSLKKSDKNSYFPQAVSLGPYHHGDEHLRPMDYHKWRAVNMVMKRTKQ-GIEM 172

Query: 101 LIELVVKSESRLRKCYEGEFYDLDSDKFSQMMLLDCCFILELLL----RYSKKRFRRWND 160
            I+ + + E R R CYEG    L S+KF+QM++LD CF+L+L       +SK  + R ND
Sbjct: 173 YIDAMKELEERARACYEGPI-GLSSNKFTQMLVLDGCFVLDLFRGAYEGFSKLGYDR-ND 232

Query: 161 PVFNTPGLLFDLRCDLMLLENQIPYFLLDEVYENVQDPLEENMSLNDLTFRFF------- 220
           PVF   G +  +R D+++LENQ+P F+L+ + E       +   +  L  RFF       
Sbjct: 233 PVFAMRGSMHSIRRDMLMLENQLPLFVLNRLLELQLGTQYQTGLVAQLAVRFFNPLMPTY 292

Query: 221 --KTMVAGDRKFVYDNFMVEADHLLEMVHSC----------FLSTYPRMETN-------- 280
              T +   ++     F   AD   E +H             L   PR+  +        
Sbjct: 293 MSSTKIENSQENNNKFFNPIADKEKEELHCLDVFRRSLLQPSLKPDPRLSRSRWSRKPLV 352

Query: 281 -DKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQTEAILRNLA 340
            DK + + L   ++L+ AGIKFK  +S +   DI+F+ G LEIP L ++  T+++  NL 
Sbjct: 353 ADKRQQQLLHCVTELREAGIKFKRRKSDR-FWDIQFKNGCLEIPKLLIHDGTKSLFSNLI 412

Query: 341 AYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILID-LEDDEEQIIQNLKWMREE 400
           AYE     S   + SYI FM +L+ S ED++ L    I+   L +D E      +  +E 
Sbjct: 413 AYEQCHIDSTNDITSYIIFMDNLIDSAEDIRYLHYYDIIEHWLGNDSEVADVFNRLCQEV 472

Query: 401 KESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAFFSAISLL 429
              L  TY + +  K++   +R   +W  L+        +  W     F A     ++L 
Sbjct: 473 AFDLENTYLSELSNKVDRYYNR---KWNVLKATLKHKYFSNPWAYFSFFAAVILLLLTLF 526

BLAST of Bhi01G000777 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 165.2 bits (417), Expect = 1.1e-40
Identity = 109/335 (32.54%), Postives = 174/335 (51.94%), Query Frame = 0

Query: 41  ITIFRAPNDISIEDKNVFVPAKVSIGPFHHGAPHLEPMENLKWRYLSTFL---KHNPSLT 100
           + I+R P  +   +K  + P  VS+GPFHHG  HL PM+  KWR ++  +   KH+  + 
Sbjct: 139 LCIYRVPQYLQENNKKSYFPQTVSLGPFHHGNKHLLPMDRHKWRAVNMVMARTKHDIEMY 198

Query: 101 LDDLIELVVKSESRLRKCYEGEFYDLDSDKFSQMMLLDCCFILELLL----RYSKKRFRR 160
           +D + EL    E R R CYEG   DL S+KFS+M++LD CF+LEL       +S+  + R
Sbjct: 199 IDAMKEL----EDRARACYEGPI-DLSSNKFSEMLVLDGCFVLELFRGADEGFSELGYDR 258

Query: 161 WNDPVFNTPGLLFDLRCDLMLLENQIPYFLLDEVYENVQDPLEENMSLNDLTFRFFKTMV 220
            NDPVF   G +  ++ D+++LENQ+P F+L+ + E       +   ++ L  RFF  ++
Sbjct: 259 -NDPVFAMRGSMHSIQRDMVMLENQLPLFVLNRLLEIQLGKRHQTGLVSRLAVRFFDPLM 318

Query: 221 AGDRKFVYDNFMVEAD--------------HLLEMVHSCFL----STYPRM--------- 280
             D      +  +E D              H L++     L    +  PR+         
Sbjct: 319 PTDEPLTKTDDSLEQDKFFNPIADKDKGELHCLDVFRRNLLRPCSNPEPRLSRMRWSWRT 378

Query: 281 ETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQTEAILRN 340
              DK + + +   ++L+ AGIKF+  ++ +   DI+F+ G LEIP L ++  T+++  N
Sbjct: 379 RVADKRQQQLIHCVTELREAGIKFRTRKTDR-FWDIRFKNGYLEIPKLLIHDGTKSLFSN 438

Query: 341 LAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKIL 342
           L A+E     S   + SYI FM +L+ S EDV+ L
Sbjct: 439 LIAFEQCHIDSSNDITSYIIFMDNLIDSSEDVRYL 466

BLAST of Bhi01G000777 vs. TAIR 10
Match: AT2G36430.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-40
Identity = 114/384 (29.69%), Postives = 192/384 (50.00%), Query Frame = 0

Query: 11  IDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVPAKVSIGPFHH 70
           I I  + +   +   LLSS  GK      + +IFR P  +   +   + P  VSIGP+H 
Sbjct: 20  IHIERMHKKLKEPPRLLSSAAGK-----PTCSIFRVPQSMIDCNGRCYEPRVVSIGPYHR 79

Query: 71  GAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEFYDLDSDKFSQ 130
           G   L+ +E  KWRYL+  L    +LTL+D ++ V   E   R+CY  E   +DS++F++
Sbjct: 80  GQTQLKMIEEHKWRYLNVLLTRTQNLTLEDYMKSVKNVEEVARECY-SETIHMDSEEFNE 139

Query: 131 MMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIPYFLLDEVYEN 190
           MM+LD CF+LEL  + +       NDP+     +L     D + LENQIP+F+L+ ++  
Sbjct: 140 MMVLDGCFLLELFRKVNNLVPFEPNDPLVAMAWVLPFFYRDFLCLENQIPFFVLETLFNL 199

Query: 191 VQ--DPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCF-----LSTY 250
            +  +  E N SL  L F FF  M+    + +     + A HLL+++ S F     L T 
Sbjct: 200 TRGDNENETNASLQSLAFAFFNNMMHRTEEDLARFKELRAKHLLDLLRSSFIPESELHTP 259

Query: 251 PRMET-NDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQTEA 310
           P      +K  S  + S SKL+ AGIK +  +  +S L ++F+ G +E+P + V     +
Sbjct: 260 PATNPGKEKMPSHIIHSISKLRRAGIKLRELKDAESFLVVRFRHGTIEMPAITVDDFMSS 319

Query: 311 ILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQNLK 370
            L N  AYE       +   +Y   +  L  + +DV+ LC + I+ +    + ++ + + 
Sbjct: 320 FLENCVAYEQCHVACSMHFTTYATLLDCLTNTYKDVEYLCDQNIIENYFGTDTELAKFVN 379

Query: 371 WM-REEKESLSGTYFAGIVQKLNE 386
            + R+    ++  Y   + +++NE
Sbjct: 380 SLGRDVAFDITQCYLKDLFEEVNE 397

BLAST of Bhi01G000777 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 3.1e-35
Identity = 114/355 (32.11%), Postives = 174/355 (49.01%), Query Frame = 0

Query: 21  SQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVPAKVSIGPFHHGAPHLEPMEN 80
           S+E  LL    GK      S  IFR P      +   + P  VSIGP+H+G  HL+ ++ 
Sbjct: 31  SKEPVLLLESAGK-----ESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQ 90

Query: 81  LKWRYLSTFLKHNPSLTLDD--LIELVVKSESRLRKCYEGEFYDLDSDKFSQMMLLDCCF 140
            K R L  FL       +++  L++ VV  E ++RK Y  E        F  MM+LD CF
Sbjct: 91  HKPRLLQLFLDEAKKKDVEENVLVKAVVDLEDKIRKSYSEELKTGHDLMF--MMVLDGCF 150

Query: 141 ILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIPYFLLDEVYENVQDPLEEN 200
           IL + L  S        DP+F+ P LL  ++ DL+LLENQ+P+F+L  +Y  V   +  +
Sbjct: 151 ILMVFLIMS-GNIELSEDPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLY--VGSKIGVS 210

Query: 201 MSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFLSTYPRMETNDKS------ 260
             LN + F FFK  +  +  +   +   +A HLL+++   FL   P    +DK+      
Sbjct: 211 SDLNRIAFHFFKNPIDKEGSYWEKHRNYKAKHLLDLIRETFL---PNTSESDKASSPHVQ 270

Query: 261 ------KSRELP-----------SASKLKTAGIKFKNARSPK-SLLDIKFQKGVLEIPPL 320
                 KS  +P           SA +L+  GIKF+  RS + S+L+++ +K  L+IP L
Sbjct: 271 VQLHEGKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQL 330

Query: 321 RVYQQTEAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILID 350
           R      +   N  A+E     S  ++ +YI FM  LL ++EDV  L   K++I+
Sbjct: 331 RFDGFISSFFLNCVAFEQFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIE 372

BLAST of Bhi01G000777 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.8e-14
Identity = 103/482 (21.37%), Postives = 178/482 (36.93%), Query Frame = 0

Query: 40  SITIFRAPNDISIEDKNVFVPAKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLD 99
           +++IF  P  +     + + P +VSIGP+H   P L  ME  K            S    
Sbjct: 42  TVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFH 101

Query: 100 DLIELVVKSESRLRKCYEGEFYDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVF 159
           DL+E +   E ++R CY  ++   + +    +M +D  F++E L  YS ++     + + 
Sbjct: 102 DLVEKLQSMEIKIRACYH-KYIGFNGETLLWIMAVDSSFLIEFLKIYSFRKV----ETLI 161

Query: 160 NTPGLLFDLRCDLMLLENQIPYFLLDEVYENVQDPLEEN------------MSLNDLTFR 219
           N  G    LR D+M++ENQIP F+L +  E   +  E                L+ L  +
Sbjct: 162 NRVGHNEILR-DIMMIENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIK 221

Query: 220 FFKTMVAGDR--------KFVYD-----------------------------NFMVEADH 279
           F    +   +         F+Y                               FM E  H
Sbjct: 222 FDDDQILKAQFQECNHILDFLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKH 281

Query: 280 LLEMVHSC------------FLSTYP-------------RMETNDKSKSRE--------- 339
             + V +              +S  P               + N+ + +R+         
Sbjct: 282 QFKRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDIE 341

Query: 340 ---------LPSASKLKTAGIKFK-NARSPKSLLDIKFQKGVLEIPPLRVYQQTEAILRN 399
                    +PS S L  AG++FK  A    S +      G   +P + +   TE +LRN
Sbjct: 342 KPPLVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRN 401

Query: 400 LAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQNLKWMRE 428
           L AYE       L    Y   ++ ++ S+EDV++L  + +L+     +++  +    M +
Sbjct: 402 LVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSK 461

BLAST of Bhi01G000777 vs. NCBI nr
Match: XP_038880915.1 (UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 857.8 bits (2215), Expect = 3.9e-245
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP
Sbjct: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF
Sbjct: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
           YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP
Sbjct: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL
Sbjct: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240

Query: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300
           STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT
Sbjct: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300

Query: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360
           EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN
Sbjct: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360

Query: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF 420
           LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF
Sbjct: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF 420

Query: 421 FSAISLLQRRYK 433
           FSAISLLQRRYK
Sbjct: 421 FSAISLLQRRYK 432

BLAST of Bhi01G000777 vs. NCBI nr
Match: XP_023526431.1 (UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526432.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526433.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526435.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526436.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526437.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526438.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526439.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724.9 bits (1870), Expect = 4.0e-205
Identity = 368/432 (85.19%), Postives = 393/432 (90.97%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           M+SS+  SHSID+ A +QGSS+EESLLSS+EGKLEAFCSSITIFRAPN+ISIED+NVFVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIEGKLEAFCSSITIFRAPNEISIEDRNVFVP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           AKVSIGPFHHGAPHLE MENLKW YLS FLK+NPS+ L  L+ELVVKSESRLRKCYE EF
Sbjct: 61  AKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLVELVVKSESRLRKCYEEEF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
           Y  DSDKFSQ+MLLDCCFILELLLR+SKKR RR ND VF TPGLLFDLRCDLMLLENQIP
Sbjct: 121 YGFDSDKFSQIMLLDCCFILELLLRFSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLL +VYENVQDP EENMSLNDLTFRFFKT+V GDR+ VYDNF VEADHLLEMVHSCFL
Sbjct: 181 YFLLKDVYENVQDPTEENMSLNDLTFRFFKTLVVGDRQLVYDNFTVEADHLLEMVHSCFL 240

Query: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300
           STYPR+ETNDKSKSRELPSASKLKTAGIK KNARS KSLLDIKFQ GVLEIPPL+VYQ+T
Sbjct: 241 STYPRVETNDKSKSRELPSASKLKTAGIKIKNARSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360
           E ILRNL AYEI Q GSD QVKSYINFMSHLLQSD+DVKIL RRKILID EDDEEQII+N
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRRKILIDQEDDEEQIIRN 360

Query: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF 420
           LKWM  EKESLSGTYFAGIVQKLNEKPDRC+ +WR LRRNPVAIG+ AV VVVVIFVAAF
Sbjct: 361 LKWM-SEKESLSGTYFAGIVQKLNEKPDRCVARWRKLRRNPVAIGIVAVLVVVVIFVAAF 420

Query: 421 FSAISLLQRRYK 433
           FSA S+LQRRYK
Sbjct: 421 FSAFSVLQRRYK 431

BLAST of Bhi01G000777 vs. NCBI nr
Match: XP_022955709.1 (UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955710.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955711.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955712.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955713.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955714.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955716.1 UPF0481 protein At3g47200-like [Cucurbita moschata])

HSP 1 Score: 721.5 bits (1861), Expect = 4.4e-204
Identity = 366/432 (84.72%), Postives = 393/432 (90.97%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           M+SS+  SHSID+ A +QGSS+EESLLSS+EGKLEAFCSSITIFRAPN+ISIED+NVFVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIEGKLEAFCSSITIFRAPNEISIEDRNVFVP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           AKVSIGPFHHGAPHLE MENLKW YLS FLK+NPS+ L  L+ELVVKSESRLRKCYE EF
Sbjct: 61  AKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLVELVVKSESRLRKCYEEEF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
           Y  DSDKFSQ+MLLDCCFILELLLR+SKKR RR ND VF TPGLLFDLRCDLMLLENQIP
Sbjct: 121 YGFDSDKFSQIMLLDCCFILELLLRFSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLL +VYENVQDP EENMSLNDLTFRFFKTMVAGDR+ VYDNFMVEADHLLEMVHSCFL
Sbjct: 181 YFLLKDVYENVQDPTEENMSLNDLTFRFFKTMVAGDRQLVYDNFMVEADHLLEMVHSCFL 240

Query: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300
           STYPR+ETNDKSKS+ELP+ASKLKTAGIK KNARS KSLLDIKFQ GVLEIPPL+VYQ+T
Sbjct: 241 STYPRVETNDKSKSKELPTASKLKTAGIKIKNARSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360
           E ILRNL AYEI Q GSD QVKSYINFMSHLLQSD+DVKIL RRKIL D EDDEEQII+N
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRRKILNDQEDDEEQIIRN 360

Query: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF 420
           LKWM  E+ESLSGTYFAGIVQKLNEKPDRC+ +WR LRR PVAIG+ AV VVVVIFVAAF
Sbjct: 361 LKWM-SERESLSGTYFAGIVQKLNEKPDRCVARWRKLRRTPVAIGIVAVLVVVVIFVAAF 420

Query: 421 FSAISLLQRRYK 433
           FSA S+LQRRYK
Sbjct: 421 FSAFSVLQRRYK 431

BLAST of Bhi01G000777 vs. NCBI nr
Match: KAG7018572.1 (UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 719.2 bits (1855), Expect = 2.2e-203
Identity = 365/432 (84.49%), Postives = 393/432 (90.97%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           M+SS+  SHSID+ A +QGSS+EESLLSS+EGKLEAFCSSITIFRAPN+ISIED+NVFVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIEGKLEAFCSSITIFRAPNEISIEDRNVFVP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           +KVSIGPFHHGAPHLE MENLKW YLS FLK+NPS+ L  L+ELVVKSESRLRKCYE EF
Sbjct: 61  SKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLVELVVKSESRLRKCYEEEF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
           Y  DSDKFSQ+MLLDCCFILELLLR+SKKR RR ND VF TPGLLFDLRCDLMLLENQIP
Sbjct: 121 YGFDSDKFSQIMLLDCCFILELLLRFSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLL +VYENVQDP EE MSLNDLTFRFFKTMVAGDR+ VYDNFMVEADHLLEMVHSCFL
Sbjct: 181 YFLLKDVYENVQDPTEEIMSLNDLTFRFFKTMVAGDRQLVYDNFMVEADHLLEMVHSCFL 240

Query: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300
           STYPR+ETNDKSKS+ELP+ASKLKTAGIK KNARS KSLLDIKFQ GVLEIPPL+VYQ+T
Sbjct: 241 STYPRVETNDKSKSKELPTASKLKTAGIKIKNARSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360
           E ILRNL AYEI Q GSD QVKSYINFMSHLLQSD+DVKIL RRKIL D EDDEEQII+N
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRRKILNDQEDDEEQIIRN 360

Query: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF 420
           LKWM  E+ESLSGTYFAGIVQKLNEKPDRC+ +WR LRRNPVAIG+ AV VVVVIFVAAF
Sbjct: 361 LKWM-SERESLSGTYFAGIVQKLNEKPDRCVARWRKLRRNPVAIGIVAVLVVVVIFVAAF 420

Query: 421 FSAISLLQRRYK 433
           FSA S+LQRRYK
Sbjct: 421 FSAFSVLQRRYK 431

BLAST of Bhi01G000777 vs. NCBI nr
Match: XP_008438948.1 (PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo])

HSP 1 Score: 689.1 bits (1777), Expect = 2.4e-194
Identity = 346/432 (80.09%), Postives = 389/432 (90.05%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           M+SS P SH+I+I  I+Q SS+EESLLSS+EGKLEA CSS+TIF+AP++I+IE +NVFVP
Sbjct: 1   MDSSTPVSHTINIPGISQESSREESLLSSIEGKLEANCSSVTIFKAPSEINIEGRNVFVP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           AKVSIGPFHHGA HL+ +ENLKWRYLSTFLKHN SLTL DLI++VVKSESRL+KCYE +F
Sbjct: 61  AKVSIGPFHHGAAHLQSVENLKWRYLSTFLKHNSSLTLQDLIKIVVKSESRLKKCYEKKF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
             LD D+FS +MLLDCCFILELLLRYSK+RF+R NDPVF TPGLLFD++CDLMLLENQIP
Sbjct: 121 CSLDRDEFSLIMLLDCCFILELLLRYSKRRFKRRNDPVFTTPGLLFDIKCDLMLLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLLDE+YE V DP EENM L+DLTFRFF+TMV GDRKF+ DNF+VEADHLLEMVHSCFL
Sbjct: 181 YFLLDEIYEKVLDPREENMFLSDLTFRFFRTMVPGDRKFMGDNFVVEADHLLEMVHSCFL 240

Query: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300
           STYP ++TNDK KS+ELPSASKLKTAGIKFKNARS KSLLDIKFQ GVLEIPPLRVYQQT
Sbjct: 241 STYPPVKTNDKLKSKELPSASKLKTAGIKFKNARSSKSLLDIKFQNGVLEIPPLRVYQQT 300

Query: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360
           EAILRNLAAYEIRQ G+D QVKSY+ FMSHLLQSD DVKILCR+KIL  LEDDEEQII+N
Sbjct: 301 EAILRNLAAYEIRQSGTDQQVKSYLKFMSHLLQSDGDVKILCRKKILYALEDDEEQIIEN 360

Query: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF 420
           LKW+RE+KESLSGTYFAGIVQKLNEKPDR + +WR LRR P AIGVAA  +VVVIF AAF
Sbjct: 361 LKWIREQKESLSGTYFAGIVQKLNEKPDRSVVRWRRLRRKPTAIGVAADLMVVVIFGAAF 420

Query: 421 FSAISLLQRRYK 433
           F+A S+LQRRYK
Sbjct: 421 FAAFSILQRRYK 432

BLAST of Bhi01G000777 vs. ExPASy TrEMBL
Match: A0A6J1GVU1 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111457630 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 2.1e-204
Identity = 366/432 (84.72%), Postives = 393/432 (90.97%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           M+SS+  SHSID+ A +QGSS+EESLLSS+EGKLEAFCSSITIFRAPN+ISIED+NVFVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIEGKLEAFCSSITIFRAPNEISIEDRNVFVP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           AKVSIGPFHHGAPHLE MENLKW YLS FLK+NPS+ L  L+ELVVKSESRLRKCYE EF
Sbjct: 61  AKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLVELVVKSESRLRKCYEEEF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
           Y  DSDKFSQ+MLLDCCFILELLLR+SKKR RR ND VF TPGLLFDLRCDLMLLENQIP
Sbjct: 121 YGFDSDKFSQIMLLDCCFILELLLRFSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLL +VYENVQDP EENMSLNDLTFRFFKTMVAGDR+ VYDNFMVEADHLLEMVHSCFL
Sbjct: 181 YFLLKDVYENVQDPTEENMSLNDLTFRFFKTMVAGDRQLVYDNFMVEADHLLEMVHSCFL 240

Query: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300
           STYPR+ETNDKSKS+ELP+ASKLKTAGIK KNARS KSLLDIKFQ GVLEIPPL+VYQ+T
Sbjct: 241 STYPRVETNDKSKSKELPTASKLKTAGIKIKNARSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360
           E ILRNL AYEI Q GSD QVKSYINFMSHLLQSD+DVKIL RRKIL D EDDEEQII+N
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRRKILNDQEDDEEQIIRN 360

Query: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF 420
           LKWM  E+ESLSGTYFAGIVQKLNEKPDRC+ +WR LRR PVAIG+ AV VVVVIFVAAF
Sbjct: 361 LKWM-SERESLSGTYFAGIVQKLNEKPDRCVARWRKLRRTPVAIGIVAVLVVVVIFVAAF 420

Query: 421 FSAISLLQRRYK 433
           FSA S+LQRRYK
Sbjct: 421 FSAFSVLQRRYK 431

BLAST of Bhi01G000777 vs. ExPASy TrEMBL
Match: A0A1S3AY98 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103483890 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.2e-194
Identity = 346/432 (80.09%), Postives = 389/432 (90.05%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           M+SS P SH+I+I  I+Q SS+EESLLSS+EGKLEA CSS+TIF+AP++I+IE +NVFVP
Sbjct: 1   MDSSTPVSHTINIPGISQESSREESLLSSIEGKLEANCSSVTIFKAPSEINIEGRNVFVP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           AKVSIGPFHHGA HL+ +ENLKWRYLSTFLKHN SLTL DLI++VVKSESRL+KCYE +F
Sbjct: 61  AKVSIGPFHHGAAHLQSVENLKWRYLSTFLKHNSSLTLQDLIKIVVKSESRLKKCYEKKF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
             LD D+FS +MLLDCCFILELLLRYSK+RF+R NDPVF TPGLLFD++CDLMLLENQIP
Sbjct: 121 CSLDRDEFSLIMLLDCCFILELLLRYSKRRFKRRNDPVFTTPGLLFDIKCDLMLLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLLDE+YE V DP EENM L+DLTFRFF+TMV GDRKF+ DNF+VEADHLLEMVHSCFL
Sbjct: 181 YFLLDEIYEKVLDPREENMFLSDLTFRFFRTMVPGDRKFMGDNFVVEADHLLEMVHSCFL 240

Query: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300
           STYP ++TNDK KS+ELPSASKLKTAGIKFKNARS KSLLDIKFQ GVLEIPPLRVYQQT
Sbjct: 241 STYPPVKTNDKLKSKELPSASKLKTAGIKFKNARSSKSLLDIKFQNGVLEIPPLRVYQQT 300

Query: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360
           EAILRNLAAYEIRQ G+D QVKSY+ FMSHLLQSD DVKILCR+KIL  LEDDEEQII+N
Sbjct: 301 EAILRNLAAYEIRQSGTDQQVKSYLKFMSHLLQSDGDVKILCRKKILYALEDDEEQIIEN 360

Query: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF 420
           LKW+RE+KESLSGTYFAGIVQKLNEKPDR + +WR LRR P AIGVAA  +VVVIF AAF
Sbjct: 361 LKWIREQKESLSGTYFAGIVQKLNEKPDRSVVRWRRLRRKPTAIGVAADLMVVVIFGAAF 420

Query: 421 FSAISLLQRRYK 433
           F+A S+LQRRYK
Sbjct: 421 FAAFSILQRRYK 432

BLAST of Bhi01G000777 vs. ExPASy TrEMBL
Match: A0A0A0L821 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G166290 PE=4 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 4.5e-186
Identity = 333/432 (77.08%), Postives = 379/432 (87.73%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           M+ S P SH+I+IS I+Q S QEESLLS +E KLEA CSS TI++AP++I+IED+NVF+P
Sbjct: 1   MDPSTPVSHTINISGISQESFQEESLLSCIERKLEANCSSFTIYKAPSEINIEDRNVFLP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           AKVSIGPFHHGAPHLE +E LKW YLSTFL H PSLTL DLI+LVVKSESR RKCYE EF
Sbjct: 61  AKVSIGPFHHGAPHLESVEKLKWHYLSTFLTHKPSLTLQDLIKLVVKSESRGRKCYEKEF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
           Y  D D+FSQ+MLLDCCFILELLLRY+K+RFRR NDPVF TPGLL+DLRCDL+LLENQIP
Sbjct: 121 YSSDRDEFSQIMLLDCCFILELLLRYTKRRFRRPNDPVFTTPGLLYDLRCDLVLLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLL+E+Y  V D LEENM L+DLT RFF+TMV GDRKF+ DNF+VEA+HLLEMV+SCFL
Sbjct: 181 YFLLEEIYAKVLDGLEENMYLSDLTSRFFRTMVPGDRKFIGDNFIVEANHLLEMVYSCFL 240

Query: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300
           STYP +ETNDK KS+ELPSASKLK AGIKFKNARS KSLLDIKFQ GVLEIPPLRVYQ+T
Sbjct: 241 STYPPVETNDKLKSKELPSASKLKAAGIKFKNARSSKSLLDIKFQNGVLEIPPLRVYQKT 300

Query: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360
           E ILRNLAAYEI QFG+DLQVKSY+NFMSHLLQSDEDVKILCR+KIL  L+D+EEQII+ 
Sbjct: 301 ETILRNLAAYEICQFGTDLQVKSYLNFMSHLLQSDEDVKILCRKKILNALKDEEEQIIEK 360

Query: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAAF 420
           LKW+RE+K+SLSGT+FAGIVQKL EKPDR + +WR LR N  AI VA V +VVVIF AAF
Sbjct: 361 LKWIREQKDSLSGTFFAGIVQKLKEKPDRSVARWRRLRSNSTAISVATVLMVVVIFGAAF 420

Query: 421 FSAISLLQRRYK 433
           F+A S+LQRRYK
Sbjct: 421 FAAFSVLQRRYK 432

BLAST of Bhi01G000777 vs. ExPASy TrEMBL
Match: A0A6J1IWZ4 (UPF0481 protein At3g47200-like OS=Cucurbita maxima OX=3661 GN=LOC111479248 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.6e-183
Identity = 331/391 (84.65%), Postives = 354/391 (90.54%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           M+SS+  SHSID+ A +QGSS+EESLLSS+E KLEAFCSSITIFRA N+ISIED+NVFVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIERKLEAFCSSITIFRATNEISIEDRNVFVP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           AKVSIGPFHHGAPHLE MENLKW YLS FLK+NPS+ L  LIELVVKSESRLRKCYE EF
Sbjct: 61  AKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLIELVVKSESRLRKCYEEEF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
           Y  DS+KFSQ+MLLDCCFILELLLRYSKKR RR ND VF TPGLLFDLRCDLMLLENQIP
Sbjct: 121 YGFDSNKFSQIMLLDCCFILELLLRYSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLL +VY NVQDP EENMSLNDLTFRFFKTMVAGDR+FVYDNFMVEADHLLEM+HSCFL
Sbjct: 181 YFLLKDVYANVQDPTEENMSLNDLTFRFFKTMVAGDRQFVYDNFMVEADHLLEMIHSCFL 240

Query: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300
           STYPRMETND SKSRELPSASKLKTAGIK KN +S KSLLDIKFQ GVLEIPPL+VYQ+T
Sbjct: 241 STYPRMETNDNSKSRELPSASKLKTAGIKIKNFKSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQN 360
           E ILRNL AYEI Q GSD QVKSYINFMSHLLQSD+DVKIL RRKIL D E+DEEQII+N
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRRKILNDQENDEEQIIRN 360

Query: 361 LKWMREEKESLSGTYFAGIVQKLNEKPDRCL 392
           LKWMR EKESLSGTYFAGIVQKLN+K DRC+
Sbjct: 361 LKWMR-EKESLSGTYFAGIVQKLNKKRDRCV 390

BLAST of Bhi01G000777 vs. ExPASy TrEMBL
Match: A0A6J1CA62 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111009362 PE=4 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 8.5e-177
Identity = 315/433 (72.75%), Postives = 368/433 (84.99%), Query Frame = 0

Query: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60
           M  S+  SH+IDI AI++  S EESLL S+E K+EAFCSSI IF+ P++ISI+++ VFVP
Sbjct: 1   MNPSRALSHAIDIPAISRERSDEESLLCSMEAKMEAFCSSIIIFKVPDEISIDNREVFVP 60

Query: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120
           AKVSIGPFHHGAPHLE ME+LKW YL  FLKHNPS+ LDDL+E V KSESR+RKCYE EF
Sbjct: 61  AKVSIGPFHHGAPHLESMEDLKWNYLCAFLKHNPSVGLDDLLEFVAKSESRVRKCYEVEF 120

Query: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180
           +DLDS KF++MM+LDCCF+LELLLR+S KR +R NDPVF TPGLL DL+ DL+LLENQIP
Sbjct: 121 HDLDSQKFARMMVLDCCFVLELLLRFSIKRLKRRNDPVFTTPGLLLDLKSDLILLENQIP 180

Query: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240
           YFLL EVYE VQD  EENM LNDL FRFF+T+VAG+R+ VYDNF  +ADHLL++VHSCFL
Sbjct: 181 YFLLREVYEKVQDSREENMPLNDLAFRFFRTIVAGERQSVYDNFQQDADHLLDIVHSCFL 240

Query: 241 STYPRMET-NDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQ 300
           STYPR+ET N+KSK+ ELP ASKLK+AGIKFKNA +PKS+LDIKFQ G LEIP L V + 
Sbjct: 241 STYPRIETKNNKSKTAELPRASKLKSAGIKFKNAVTPKSVLDIKFQNGGLEIPTLEVSKH 300

Query: 301 TEAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRRKILIDLEDDEEQIIQ 360
           TE IL+NL AYEI Q GS  QVKSY++FMSHLLQSDED+K+LC RKILI+LE DE QII 
Sbjct: 301 TETILKNLIAYEICQIGSAQQVKSYVDFMSHLLQSDEDMKLLCGRKILINLEKDETQIIA 360

Query: 361 NLKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAA 420
           NLKWMR++K +LSGTYFAG+VQKLNE PDR +  WR LRRNPVAIGV AVW +VVIFVAA
Sbjct: 361 NLKWMRQQKANLSGTYFAGVVQKLNEPPDRFIVWWRRLRRNPVAIGVVAVWALVVIFVAA 420

Query: 421 FFSAISLLQRRYK 433
           FFSA+SLLQRRY+
Sbjct: 421 FFSALSLLQRRYR 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G22540.15.4e-4332.02Plant protein of unknown function (DUF247) [more]
AT4G31980.17.1e-4327.93unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT3G50140.13.9e-4130.64Plant protein of unknown function (DUF247) [more]
AT3G50130.11.1e-4032.54Plant protein of unknown function (DUF247) [more]
AT2G36430.11.5e-4029.69Plant protein of unknown function (DUF247) [more]
Match NameE-valueIdentityDescription
Q9SD533.1e-3532.11UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8971.8e-1421.37Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
XP_038880915.13.9e-245100.00UPF0481 protein At3g47200-like [Benincasa hispida][more]
XP_023526431.14.0e-20585.19UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526432.1 UPF0... [more]
XP_022955709.14.4e-20484.72UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955710.1 UPF0481 prot... [more]
KAG7018572.12.2e-20384.49UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_008438948.12.4e-19480.09PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1GVU12.1e-20484.72UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111457630 PE=... [more]
A0A1S3AY981.2e-19480.09UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103483890 PE=4 SV=1[more]
A0A0A0L8214.5e-18677.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G166290 PE=4 SV=1[more]
A0A6J1IWZ41.6e-18384.65UPF0481 protein At3g47200-like OS=Cucurbita maxima OX=3661 GN=LOC111479248 PE=4 ... [more]
A0A6J1CA628.5e-17772.75UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111009362 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 43..420
e-value: 2.4E-89
score: 300.4
IPR004158Protein of unknown function DUF247, plantPANTHERPTHR31549PROTEIN, PUTATIVE (DUF247)-RELATED-RELATEDcoord: 18..425
NoneNo IPR availablePANTHERPTHR31549:SF38SUBFAMILY NOT NAMEDcoord: 18..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000777Bhi01M000777mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane