Bhi01G000775 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000775
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionUbiquitin-conjugating enzyme E2 32
Locationchr1: 20390477 .. 20393130 (+)
RNA-Seq ExpressionBhi01G000775
SyntenyBhi01G000775
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCCATTCATCTCTACGGTTTGGCTTTGATTTCGATTCAAATCCTCTCGTTTGGTTCCGGGGGTCGCTTCTTTTTCTTTTTCGATCAGATAATTGTAGTCCTGTGATCGGGTGGTGGTTGCAGTTGGATCGGATCTGCGATTGAATTGCGAAGCTATGGCGGAGGATAAGTACAATCTAAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGACGATTTCATGAGTCTTCCTCTTGAGGTTTGTTGCTTCTGAATTTCGAATTCTGTTTTGAAATTTACCCTAATGGGTATTGTTCTTGTTTGGTCGGTTTTACATTTTAATGGTTTGGACATCTTTGTTGAATGAATTGATTCATTGTGATCATCTACAATTATTGAACTGGTATGATGTATGTGGAAGTGTGGACGGAAATCGATTATTTAATTTGGTGTTCTTTAATTTTTCTTGCCTGCTCGTGTCTGCTACTGTTTGCATATGAATGTTCCGTTAGGGCTCAGAAAGGAAGAAAAATGGATATATAAAATTATTAACCTTCTTTTTTCTTTTGATTAAATTTTTATTATGCCATTCATTCGAATTTCGAGTTTACAATCTTCGAAAATTTGGGGCAGTCCTTTGTTTTAAATAAACTCTCGCTCTCTAGTTAGGCTAATAATCAATCTGTTTTAATTGATTACTAAATCTACAAACACGCGTGCATTCAGTCCTTTTCTGGATAAATCTGCATGCATCTTATTCTATTCTCATGTGTCTTGATTTTTATAATTGCATTGTCCTCATTGCATTTCGGATCAAGGGGAAAAAAGAAAGAAAATACGAGTTAGGCAGCCATGGGAAGCTTTCTTGACGAATACATCAGTTCTTGTATTTATCCGCGTAATTTTGGTTTTTTAATCCTTATAGAATTTAAGGGTTTTGCCAAAAATCTCTATTAAATTTGAGGCTTAATGTTGTTTTTTCAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCTGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGTTGTTAACCGTAAGATATAAATCTCTCTTCATGCTTGTTTCCTTTCTGTCGCTTCTTGGCTTGGGATGTTTGGTTATGATTTTGAAATGGTCATAAATCATTATAATCATATTCAATACACTTTTAAACATAAAACACAATTTTCGTACTATCAAAATTCACTTTGAATGATTAAAAGCAATGTTTTGGAGTGAACACATGACTAAGATGATTATAACAATTTCATACTTCTTTTGTTGTGTATTTTGACAAGTGATTTTATACTTCATTGAAGAGCTGATCAATATCAATTCTTTTCTTTCAGCCGAATGGTCGCTTTGAAACCCAAACGAAGATATGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCATCATGGAGTGGTGCGTTCTTCACATCCTTTATTCAACTTGTCATGTCATGTCATGTTATGTTAAACTTATAGGATTTCCATTGTCTGGTTTCTACATTTTCATTTAGTTGCTTCAAGTTCCACACGCTCTGATCATCGTTCCTTTTTTCCCACTTCCCCTTTTCTGTTGATTACTGTTTGTAGTGCGTACTGCATTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTATTGGCCATCAAATCTCGAGAAGCACCCCCAAAATTTGGTACTCCTGAACGTCAAAAGCTAATTGACGAGGTAGAGTTCTGTCAATATTTTGTAAGCTATCGAGTAATGTACCGAATTTCCAACATGTGATATGATATTCTTAAGTAATTAGCTTCTTTACTACATCACAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTCCCTCAATCGGGTTCTTCAGATGTCTCCGGGGAGAAACCCACCAATGAAGAGGCTGATGTCCAGGTAACTTCTGAACCTGCTGAACTTGCAGCTTCTGGGGAAGAGCTGCAGAATCCACCTGCAAATGACAGGGTCGTTGAGGAAGTAGTACCAGAAGTCCGACCAGAAGTGGTTGCGAGAGTGGCAAGAGAGGTGCCATCTAGTCGCCCTCCCGCTCAGGCCGAGCAGCGGAGGCTAGAGACGGGAGTTCATAAACCAGCTGATGACCGGTTGTTCACATGGGCTGCTATCGGTCTCACTCTTGCAATTGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTCTTCATGGATGAATCTTAGATTACAGATATATTTGTTGGTTGATAGCTTTGCTATGAGACACGGGTCTTAATGTGTATTAATAGTTTTAGCGTAAATTCAATTGGCTTATCATTCGACAAAGAAAAAAGCGATCTTATGTGTTATGGTGCCGCCGTCTCCATTATTTGTAGTTAAATAATAACATAAGCAAAATTGCTTGGCTCAAAGAGTGTGCCTACAAACCATTTCATTACCTCGTTGTCTGTTTTATGTACAAGGTATTATTTGCTATACTGGTTTATTCTTATTGTTTATTTGATTTTCTTCTCAAGTTAAA

mRNA sequence

CCCCCATTCATCTCTACGGTTTGGCTTTGATTTCGATTCAAATCCTCTCGTTTGGTTCCGGGGGTCGCTTCTTTTTCTTTTTCGATCAGATAATTGTAGTCCTGTGATCGGGTGGTGGTTGCAGTTGGATCGGATCTGCGATTGAATTGCGAAGCTATGGCGGAGGATAAGTACAATCTAAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGACGATTTCATGAGTCTTCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCTGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGTTGTTAACCCCGAATGGTCGCTTTGAAACCCAAACGAAGATATGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCATCATGGAGTGTGCGTACTGCATTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTATTGGCCATCAAATCTCGAGAAGCACCCCCAAAATTTGGTACTCCTGAACGTCAAAAGCTAATTGACGAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTCCCTCAATCGGGTTCTTCAGATGTCTCCGGGGAGAAACCCACCAATGAAGAGGCTGATGTCCAGGTAACTTCTGAACCTGCTGAACTTGCAGCTTCTGGGGAAGAGCTGCAGAATCCACCTGCAAATGACAGGGTCGTTGAGGAAGTAGTACCAGAAGTCCGACCAGAAGTGGTTGCGAGAGTGGCAAGAGAGGTGCCATCTAGTCGCCCTCCCGCTCAGGCCGAGCAGCGGAGGCTAGAGACGGGAGTTCATAAACCAGCTGATGACCGGTTGTTCACATGGGCTGCTATCGGTCTCACTCTTGCAATTGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTCTTCATGGATGAATCTTAGATTACAGATATATTTGTTGGTTGATAGCTTTGCTATGAGACACGGGTCTTAATGTGTATTAATAGTTTTAGCGTAAATTCAATTGGCTTATCATTCGACAAAGAAAAAAGCGATCTTATGTGTTATGGTGCCGCCGTCTCCATTATTTGTAGTTAAATAATAACATAAGCAAAATTGCTTGGCTCAAAGAGTGTGCCTACAAACCATTTCATTACCTCGTTGTCTGTTTTATGTACAAGGTATTATTTGCTATACTGGTTTATTCTTATTGTTTATTTGATTTTCTTCTCAAGTTAAA

Coding sequence (CDS)

ATGGCGGAGGATAAGTACAATCTAAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGACGATTTCATGAGTCTTCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCTGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGTTGTTAACCCCGAATGGTCGCTTTGAAACCCAAACGAAGATATGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCATCATGGAGTGTGCGTACTGCATTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTATTGGCCATCAAATCTCGAGAAGCACCCCCAAAATTTGGTACTCCTGAACGTCAAAAGCTAATTGACGAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTCCCTCAATCGGGTTCTTCAGATGTCTCCGGGGAGAAACCCACCAATGAAGAGGCTGATGTCCAGGTAACTTCTGAACCTGCTGAACTTGCAGCTTCTGGGGAAGAGCTGCAGAATCCACCTGCAAATGACAGGGTCGTTGAGGAAGTAGTACCAGAAGTCCGACCAGAAGTGGTTGCGAGAGTGGCAAGAGAGGTGCCATCTAGTCGCCCTCCCGCTCAGGCCGAGCAGCGGAGGCTAGAGACGGGAGTTCATAAACCAGCTGATGACCGGTTGTTCACATGGGCTGCTATCGGTCTCACTCTTGCAATTGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTCTTCATGGATGAATCTTAG

Protein sequence

MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVAREVPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Homology
[Close]
BLAST of Bhi01G000775 vs. TAIR 10
Match: AT3G17000.1 (ubiquitin-conjugating enzyme 32 )

HSP 1 Score: 396.7 bits (1018), Expect = 1.6e-110
Identity = 207/310 (66.77%), Postives = 240/310 (77.42%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPT+PNGALGS+DY K+ERR LAIKSRE PPK+G+PERQK+IDEIH+Y+LSKA  VP+  
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240
             + S       EA  QV  EP E     EE ++    D +V++ + E   E V   A  
Sbjct: 181 PLECSQAPSIVSEAHSQV--EPQEAITVVEE-RSIATTDTIVDDQIIEETAEAVNTAASV 240

Query: 241 VPSSRP-PA---------QAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKA 300
           VP++ P PA           EQR       KP DDRLFTWAA+GLT+AI+VLLLKKF+K+
Sbjct: 241 VPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKS 300

[Close]
BLAST of Bhi01G000775 vs. TAIR 10
Match: AT1G17280.1 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 121.3 bits (303), Expect = 1.3e-27
Identity = 70/198 (35.35%), Postives = 108/198 (54.55%), Query Frame = 0 

Query: 13  VKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNGALGS 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P    GS
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP--TTGS 126

Query: 133 LDYKKEERRVLAIKSREAPPKFGTPERQKL----IDEIHEYMLSK-------APPVPQSG 192
           ++    E++ LA  S     K  TP  +KL    +++ ++  L++        P  PQ  
Sbjct: 127 VNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKS 186

Query: 193 SSDVSGEK---PTNEEAD 196
            + V  EK   PT  +++
Sbjct: 187 DTKVESEKTIDPTKGDSE 200

[Close]
BLAST of Bhi01G000775 vs. TAIR 10
Match: AT1G17280.2 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 121.3 bits (303), Expect = 1.3e-27
Identity = 70/198 (35.35%), Postives = 108/198 (54.55%), Query Frame = 0 

Query: 13  VKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNGALGS 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P    GS
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP--TTGS 126

Query: 133 LDYKKEERRVLAIKSREAPPKFGTPERQKL----IDEIHEYMLSK-------APPVPQSG 192
           ++    E++ LA  S     K  TP  +KL    +++ ++  L++        P  PQ  
Sbjct: 127 VNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKS 186

Query: 193 SSDVSGEK---PTNEEAD 196
            + V  EK   PT  +++
Sbjct: 187 DTKVESEKTIDPTKGDSE 200

[Close]
BLAST of Bhi01G000775 vs. TAIR 10
Match: AT5G50430.1 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 120.6 bits (301), Expect = 2.2e-27
Identity = 61/187 (32.62%), Postives = 102/187 (54.55%), Query Frame = 0 

Query: 13  VKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKEERRVLAIKSREAPPKFGTPER--QKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPT 192
           +    E++ LA  S     K  T  +   + +++  +  +++     Q  ++  + + P 
Sbjct: 127 NTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQ 186

Query: 193 NEEADVQ 198
            + A V+
Sbjct: 187 KDNAKVE 192

[Close]
BLAST of Bhi01G000775 vs. TAIR 10
Match: AT5G50430.2 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 120.6 bits (301), Expect = 2.2e-27
Identity = 61/187 (32.62%), Postives = 102/187 (54.55%), Query Frame = 0 

Query: 13  VKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKEERRVLAIKSREAPPKFGTPER--QKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPT 192
           +    E++ LA  S     K  T  +   + +++  +  +++     Q  ++  + + P 
Sbjct: 127 NTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQ 186

Query: 193 NEEADVQ 198
            + A V+
Sbjct: 187 KDNAKVE 192

[Close]
BLAST of Bhi01G000775 vs. ExPASy Swiss-Prot
Match: Q9LSP7 (Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 2.3e-109
Identity = 207/310 (66.77%), Postives = 240/310 (77.42%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPT+PNGALGS+DY K+ERR LAIKSRE PPK+G+PERQK+IDEIH+Y+LSKA  VP+  
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240
             + S       EA  QV  EP E     EE ++    D +V++ + E   E V   A  
Sbjct: 181 PLECSQAPSIVSEAHSQV--EPQEAITVVEE-RSIATTDTIVDDQIIEETAEAVNTAASV 240

Query: 241 VPSSRP-PA---------QAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKA 300
           VP++ P PA           EQR       KP DDRLFTWAA+GLT+AI+VLLLKKF+K+
Sbjct: 241 VPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKS 300

[Close]
BLAST of Bhi01G000775 vs. ExPASy Swiss-Prot
Match: Q9JJZ4 (Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2)

HSP 1 Score: 211.1 bits (536), Expect = 1.8e-53
Identity = 94/146 (64.38%), Postives = 118/146 (80.82%), Query Frame = 0 

Query: 3   EDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRE 149
           T   GA+GSLDY  EERR LA KS++
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

[Close]
BLAST of Bhi01G000775 vs. ExPASy Swiss-Prot
Match: Q9Y385 (Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2)

HSP 1 Score: 206.1 bits (523), Expect = 5.7e-52
Identity = 132/320 (41.25%), Postives = 178/320 (55.62%), Query Frame = 0 

Query: 3   EDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRE--------------APPKFGTPERQ--KLIDEIH 182
           T   GA+GSLDY  EERR LA KS++               P K G+   Q  +   E+ 
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSDSSQADQEAKELA 181

Query: 183 EYMLSKAPPVPQSG----SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVV 242
             +  KA  V  SG     SD++      +  D   T+     A++   LQN  A     
Sbjct: 182 RQISFKA-EVNSSGKTISESDLNHSFSLTDLQDDIPTTFQGATASTSYGLQNSSAASF-- 241

Query: 243 EEVVPEVRPEVVARVAREVPSSRPPAQAEQRRLETGV-----HKPADDRLFTWAA----I 294
                    + VA+     P  R   Q  QRRL T       H+P D+      +    +
Sbjct: 242 -----HQPTQPVAKNTSMSPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHTDHGGSAVLIV 301

[Close]
BLAST of Bhi01G000775 vs. ExPASy Swiss-Prot
Match: Q6FQK7 (Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=UBC6 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-33
Identity = 61/135 (45.19%), Postives = 91/135 (67.41%), Query Frame = 0 

Query: 12  AVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYP 71
           A KR+ +E K M  NP    ++ P EENI EW + I GP D+ ++GG YHG +  P++YP
Sbjct: 6   AQKRLTKEYKMMVENPPPFIIARPNEENILEWHYVISGPPDTPYDGGQYHGTLTFPSDYP 65

Query: 72  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           +KPP+  ++TPNGRF+  T++CLS+S++HP+ W P WSV T L  L++FM T      GS
Sbjct: 66  YKPPAIRMITPNGRFKENTRLCLSMSDYHPDTWNPGWSVATILNGLLSFM-TGDESTTGS 125

Query: 132 LDYKKEERRVLAIKS 147
           +   ++E+++LA KS
Sbjct: 126 ITTTQQEKKILAKKS 139

[Close]
BLAST of Bhi01G000775 vs. ExPASy Swiss-Prot
Match: Q74Z34 (Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=UBC6 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.5e-31
Identity = 59/137 (43.07%), Postives = 88/137 (64.23%), Query Frame = 0 

Query: 12  AVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYP 71
           A KR+ +E K M  NP    ++ P E+NI  W + I GP ++ +E G YHG +  P +YP
Sbjct: 6   AYKRLSKEYKMMTENPPPYIVAAPKEDNILVWHYVITGPPETPYEDGQYHGTLVFPNDYP 65

Query: 72  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           F PP+  +LTPNGRF   T++CLS+S++HP+ W PSWSV T L  L++FM T+   ++G+
Sbjct: 66  FNPPAIRMLTPNGRFRENTRLCLSMSDYHPDTWNPSWSVATILTGLLSFMTTD-ESSIGT 125

Query: 132 LDYKKEERRVLAIKSRE 149
           ++     +R  A KS+E
Sbjct: 126 MESTDSTKRKYAAKSKE 141

[Close]
BLAST of Bhi01G000775 vs. NCBI nr
Match: XP_038895230.1 (ubiquitin-conjugating enzyme E2 32 [Benincasa hispida])

HSP 1 Score: 600.5 bits (1547), Expect = 7.9e-168
Identity = 301/301 (100.00%), Postives = 301/301 (100.00%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240
           SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE
Sbjct: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240

Query: 241 VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 300
           VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE
Sbjct: 241 VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 300

Query: 301 S 302
           S
Sbjct: 301 S 301

[Close]
BLAST of Bhi01G000775 vs. NCBI nr
Match: KAA0049541.1 (ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa] >TYK16218.1 ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa])

HSP 1 Score: 553.1 bits (1424), Expect = 1.4e-153
Identity = 279/301 (92.69%), Postives = 286/301 (95.02%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240
           SSDVS EKPTNEE+DVQVTSEPAELAA+GEELQNPP  +R+VEE VPEV+PE    VAR 
Sbjct: 181 SSDVSDEKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPEVQPE----VARV 240

Query: 241 VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 300
            PSSRP  QAEQ+R ET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE
Sbjct: 241 APSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 297

Query: 301 S 302
           S
Sbjct: 301 S 297

[Close]
BLAST of Bhi01G000775 vs. NCBI nr
Match: XP_008438945.1 (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo])

HSP 1 Score: 552.0 bits (1421), Expect = 3.2e-153
Identity = 278/301 (92.36%), Postives = 286/301 (95.02%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240
           SSDVS +KPTNEE+DVQVTSEPAELAA+GEELQNPP  +R+VEE VPEV+PE    VAR 
Sbjct: 181 SSDVSDQKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPEVQPE----VARV 240

Query: 241 VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 300
            PSSRP  QAEQ+R ET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE
Sbjct: 241 APSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 297

Query: 301 S 302
           S
Sbjct: 301 S 297

[Close]
BLAST of Bhi01G000775 vs. NCBI nr
Match: XP_004134243.1 (ubiquitin-conjugating enzyme E2 32 [Cucumis sativus] >KGN57162.1 hypothetical protein Csa_009813 [Cucumis sativus])

HSP 1 Score: 547.0 bits (1408), Expect = 1.0e-151
Identity = 278/301 (92.36%), Postives = 283/301 (94.02%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240
           S+DVSGEKPTNEEADVQVTSE AELAA+ EELQN P  +R+VEE VPEV+PE    VAR 
Sbjct: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIVEEAVPEVQPE----VARV 240

Query: 241 VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 300
            PSSRP  Q EQ R ET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE
Sbjct: 241 APSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 297

Query: 301 S 302
           S
Sbjct: 301 S 297

[Close]
BLAST of Bhi01G000775 vs. NCBI nr
Match: XP_022138234.1 (ubiquitin-conjugating enzyme E2 32 [Momordica charantia])

HSP 1 Score: 510.0 bits (1312), Expect = 1.4e-140
Identity = 263/308 (85.39%), Postives = 274/308 (88.96%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAE+KYNLKNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEEKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPSDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFG+PERQKLIDEIHEY+LSKAPP+PQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGSPERQKLIDEIHEYILSKAPPIPQ-- 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPE-------VRPEV 240
                   P+  +ADVQVTSEPAELAA+GEELQNPPA DR+VEE VPE        +PEV
Sbjct: 181 --------PSPSDADVQVTSEPAELAAAGEELQNPPAGDRIVEEAVPEEVTTNANPQPEV 240

Query: 241 VARVAREVPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAH 300
           V RVAREVPSSR      Q R ET V KPADDRLFTWAA+GLTLAIVVLLLKKFMKASAH
Sbjct: 241 V-RVAREVPSSRAATAQVQMRAETRVPKPADDRLFTWAAVGLTLAIVVLLLKKFMKASAH 297

Query: 301 GSVFMDES 302
           GSVFMDES
Sbjct: 301 GSVFMDES 297

[Close]
BLAST of Bhi01G000775 vs. ExPASy TrEMBL
Match: A0A5A7U7K5 (Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001470 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.0e-154
Identity = 279/301 (92.69%), Postives = 286/301 (95.02%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240
           SSDVS EKPTNEE+DVQVTSEPAELAA+GEELQNPP  +R+VEE VPEV+PE    VAR 
Sbjct: 181 SSDVSDEKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPEVQPE----VARV 240

Query: 241 VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 300
            PSSRP  QAEQ+R ET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE
Sbjct: 241 APSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 297

Query: 301 S 302
           S
Sbjct: 301 S 297

[Close]
BLAST of Bhi01G000775 vs. ExPASy TrEMBL
Match: A0A1S3AXJ7 (ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.6e-153
Identity = 278/301 (92.36%), Postives = 286/301 (95.02%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240
           SSDVS +KPTNEE+DVQVTSEPAELAA+GEELQNPP  +R+VEE VPEV+PE    VAR 
Sbjct: 181 SSDVSDQKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPEVQPE----VARV 240

Query: 241 VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 300
            PSSRP  QAEQ+R ET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE
Sbjct: 241 APSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 297

Query: 301 S 302
           S
Sbjct: 301 S 297

[Close]
BLAST of Bhi01G000775 vs. ExPASy TrEMBL
Match: A0A0A0L954 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G166270 PE=4 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.0e-152
Identity = 278/301 (92.36%), Postives = 283/301 (94.02%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240
           S+DVSGEKPTNEEADVQVTSE AELAA+ EELQN P  +R+VEE VPEV+PE    VAR 
Sbjct: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIVEEAVPEVQPE----VARV 240

Query: 241 VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 300
            PSSRP  Q EQ R ET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE
Sbjct: 241 APSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 297

Query: 301 S 302
           S
Sbjct: 301 S 297

[Close]
BLAST of Bhi01G000775 vs. ExPASy TrEMBL
Match: A0A6J1C9I9 (ubiquitin-conjugating enzyme E2 32 OS=Momordica charantia OX=3673 GN=LOC111009459 PE=4 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 6.8e-141
Identity = 263/308 (85.39%), Postives = 274/308 (88.96%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAE+KYNLKNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEEKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPSDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFG+PERQKLIDEIHEY+LSKAPP+PQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGSPERQKLIDEIHEYILSKAPPIPQ-- 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPE-------VRPEV 240
                   P+  +ADVQVTSEPAELAA+GEELQNPPA DR+VEE VPE        +PEV
Sbjct: 181 --------PSPSDADVQVTSEPAELAAAGEELQNPPAGDRIVEEAVPEEVTTNANPQPEV 240

Query: 241 VARVAREVPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAH 300
           V RVAREVPSSR      Q R ET V KPADDRLFTWAA+GLTLAIVVLLLKKFMKASAH
Sbjct: 241 V-RVAREVPSSRAATAQVQMRAETRVPKPADDRLFTWAAVGLTLAIVVLLLKKFMKASAH 297

Query: 301 GSVFMDES 302
           GSVFMDES
Sbjct: 301 GSVFMDES 297

[Close]
BLAST of Bhi01G000775 vs. ExPASy TrEMBL
Match: A0A6J1FCW1 (ubiquitin-conjugating enzyme E2 32-like OS=Cucurbita moschata OX=3662 GN=LOC111444487 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 8.9e-141
Identity = 257/304 (84.54%), Postives = 272/304 (89.47%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRIL+EVKEMQANPSDDFMSLPLE+NIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREA PKFGTPERQKLIDEIHEY+LSKAPP PQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180

Query: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPE---VRPEVVARV 240
           S D S EKP N+EADV+VTSEP E AA+GEE QNPP +DR+VEE +PE   +   +    
Sbjct: 181 SPDDSEEKPANKEADVEVTSEPTEPAATGEEQQNPPGSDRIVEEAIPEETSINTNLQQAA 240

Query: 241 AREVPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVF 300
           ARE PS RP   A Q R ET V+KPADDRLFTWAA+GLTLAIVVLLLKKF+KASAHGSVF
Sbjct: 241 AREAPSDRP---AVQLRTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASAHGSVF 300

Query: 301 MDES 302
           MDES
Sbjct: 301 MDES 301

The following BLAST results are available for this feature:
BLAST of Bhi01G000775 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G17000.11.6e-11066.77ubiquitin-conjugating enzyme 32 [more]
AT1G17280.11.3e-2735.35ubiquitin-conjugating enzyme 34 [more]
AT1G17280.21.3e-2735.35ubiquitin-conjugating enzyme 34 [more]
AT5G50430.12.2e-2732.62ubiquitin-conjugating enzyme 33 [more]
AT5G50430.22.2e-2732.62ubiquitin-conjugating enzyme 33 [more]
BLAST of Bhi01G000775 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LSP72.3e-10966.77Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2... [more]
Q9JJZ41.8e-5364.38Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2[more]
Q9Y3855.7e-5241.25Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2[more]
Q6FQK72.7e-3345.19Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 13... [more]
Q74Z342.5e-3143.07Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii (strain ATCC 10895 / CBS 10... [more]
BLAST of Bhi01G000775 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038895230.17.9e-168100.00ubiquitin-conjugating enzyme E2 32 [Benincasa hispida][more]
KAA0049541.11.4e-15392.69ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa] >TYK16218.1 ubiqui... [more]
XP_008438945.13.2e-15392.36PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo][more]
XP_004134243.11.0e-15192.36ubiquitin-conjugating enzyme E2 32 [Cucumis sativus] >KGN57162.1 hypothetical pr... [more]
XP_022138234.11.4e-14085.39ubiquitin-conjugating enzyme E2 32 [Momordica charantia][more]
BLAST of Bhi01G000775 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7U7K57.0e-15492.69Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AXJ71.6e-15392.36ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 ... [more]
A0A0A0L9545.0e-15292.36UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G166270 PE... [more]
A0A6J1C9I96.8e-14185.39ubiquitin-conjugating enzyme E2 32 OS=Momordica charantia OX=3673 GN=LOC11100945... [more]
A0A6J1FCW18.9e-14184.54ubiquitin-conjugating enzyme E2 32-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 14..174
e-value: 6.9E-28
score: 108.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..215
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..197
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..257
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 7..301
NoneNo IPR availablePANTHERPTHR24068:SF303BNAC01G21910D PROTEINcoord: 7..301
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 15..126
e-value: 5.6E-20
score: 71.4
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 11..166
score: 37.480679
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 13..152
e-value: 6.91867E-45
score: 147.349
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 2..173
e-value: 7.5E-47
score: 161.1
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 12..134

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000775Bhi01M000775mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0061631 ubiquitin conjugating enzyme activity