Bhi01G000752 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000752
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionChaperone protein DnaJ
Locationchr1: 19507937 .. 19509818 (+)
RNA-Seq ExpressionBhi01G000752
SyntenyBhi01G000752
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTTGGTTTCATTCCACATTACCAATGCCATAATACCATATACCAACCGAATCCACTTTCTTTGAAATGAAAAAGAAATCATATTATCAAGTCTACCCCGCAAGTGTTGGCCCTTCCTTCTCCCTTTTCCCTCTTTCTTTAACTGCCTCCATCTTCTTCAATCTCAATTCTCACTACCTGAAACTAACAAATTCCCCATTTCCCCATTTTTCTCTGAATCTTAATGGGTCCTGATTCCGATTTCAAATCCCATTTGGCCACTGAAATTAGCTCCTTCTCCTCCCGTACTGTCTCTTGCTCTCACCGCCATTTCTCCCTCCCTTTCATCGATTGGTATCGCCTTTTTGGGGTACGTTTTTCCCTTTCCTCTTATGGGTTTTCTTTCTTTTCTTCCCTTCTCTCTTAATCCATTCTCATAGGTCCAACAAGACGCTGGAATCGATTCCATTCGGAGGCGATATCTCAAGCTTGGTGGGTTTCTCTTTTCTATCTCTCTCCATTTCCATTTCGTTTTGTTTTGATTCGGAATGTGCTTTGGATTTGCAGCTCTGCAACTTCATCCAGACAAAAACTCGCACCCCAAGGCCGAAATCGCCTTCAAGCTTGTCTCTGAGGTTCGATTCCATTCATTTTCTTGGGAATCCCACTTTCTGCCATATATCTGTTCTACATTTTTGTGATTTTGCTTTTTAGTGTTTTTTTTTCTTTTTCATGGCGATCCATTAATGTGAATCTTGCATTTAATTTCTGTATTCACGTACTACTCGTGAATATTGATGTATTCATTACTGTTTGTGTTCTTCACTTTAATGCTAAATCAATTCTGAATGGTGAGAACTGTGAATGAAAATGAGGGATTGTTTTGTTTGTGTTTGTGCTTGTAGGGATATGGTTGTTTGAGTGATAATGTGAAGAGAAGGGAATTTGATTTGGATAGGAAGAAGAAGTTTTGCCCTGATTGCAACACAATCCCTTATAGAAATTTCACCTCTGCTAGAAACTTGAATGTACTAGGAGGTTTTCACAACAACAACAACATCAACAACATTCATGGAGATTTGAGAGATATGAAAGAGAGATTAAGAGAGGAAGCTAATGTGATTAAGAATTGTTTGAGGACTAATAACTCTGCAGCAAACAGAGAAACTTCACTATTCAATCCACCTGGTTTCACCATGTTTCATTACAAAAACCACAAAGAAACCCCAATCTTCAATCCTTCTGATTATGTTTGTCAAGGCTACCCACATTTCCGAACCCGAATCCACCCGAAACCGCAGAGTTTTCGGTGTTTTAGAACTGGAAATGCATTGAAGTATGAACAAGGTAGAGGGAAGTATGAGTATCCGGTGTTTGAAATTAGTAGATCAGAAAGTTTTCTTTTACAGAAACAATCTGCTTTTGTTTATTCAAATGTTAACAAACCACATTGGTGAACTGACTTCCATTCATTCAATGCCATTTCTTTTCTTCTTTTCTGGGTAAGTAAATGCATTATTAAAAAACAAATCGTTTTATTTTCGAGATTTTTTTTTTTTTTTTCAAATTAAAGCAAAGTTCTCTCTGTCATTTGAATTGGAGGGAAAGGGAAACCCTTTTGAATAACAATTCTTTTCTTTTTTTTTTAATACTTTCTAAATGTATTATGATTAGTTTAAATCTTTAATAAAGAAAGTAAGAAAGTGGGTAACAAATTTTGTTTTTTTGGAACTTGGGTTTTGTTTTAGTTTAATCATGTAGATTTATTTTTGAATATTTGTTATGTGTTCAAAGGGTTAATGATGGAGAATTCTGAGGGGTCAAGTCAATGTGATGGTAAAGTTTTTAGATGGGTCTGCTAAAAGTGAGAGAGTGAAAGAGTCAGTTGAGGGAAAGTGGGGT

mRNA sequence

ATCTTGGTTTCATTCCACATTACCAATGCCATAATACCATATACCAACCGAATCCACTTTCTTTGAAATGAAAAAGAAATCATATTATCAAGTCTACCCCGCAAGTGTTGGCCCTTCCTTCTCCCTTTTCCCTCTTTCTTTAACTGCCTCCATCTTCTTCAATCTCAATTCTCACTACCTGAAACTAACAAATTCCCCATTTCCCCATTTTTCTCTGAATCTTAATGGGTCCTGATTCCGATTTCAAATCCCATTTGGCCACTGAAATTAGCTCCTTCTCCTCCCGTACTGTCTCTTGCTCTCACCGCCATTTCTCCCTCCCTTTCATCGATTGGTATCGCCTTTTTGGGGTCCAACAAGACGCTGGAATCGATTCCATTCGGAGGCGATATCTCAAGCTTGCTCTGCAACTTCATCCAGACAAAAACTCGCACCCCAAGGCCGAAATCGCCTTCAAGCTTGTCTCTGAGGGATATGGTTGTTTGAGTGATAATGTGAAGAGAAGGGAATTTGATTTGGATAGGAAGAAGAAGTTTTGCCCTGATTGCAACACAATCCCTTATAGAAATTTCACCTCTGCTAGAAACTTGAATGTACTAGGAGGTTTTCACAACAACAACAACATCAACAACATTCATGGAGATTTGAGAGATATGAAAGAGAGATTAAGAGAGGAAGCTAATGTGATTAAGAATTGTTTGAGGACTAATAACTCTGCAGCAAACAGAGAAACTTCACTATTCAATCCACCTGGTTTCACCATGTTTCATTACAAAAACCACAAAGAAACCCCAATCTTCAATCCTTCTGATTATGTTTGTCAAGGCTACCCACATTTCCGAACCCGAATCCACCCGAAACCGCAGAGTTTTCGGTGTTTTAGAACTGGAAATGCATTGAAGTATGAACAAGGTAGAGGGAAGTATGAGTATCCGGTGTTTGAAATTAGTAGATCAGAAAGTTTTCTTTTACAGAAACAATCTGCTTTTGTTTATTCAAATGTTAACAAACCACATTGGTGAACTGACTTCCATTCATTCAATGCCATTTCTTTTCTTCTTTTCTGGGTAAGTAAATGCATTATTAAAAAACAAATCGTTTTATTTTCGAGATTTTTTTTTTTTTTTTCAAATTAAAGCAAAGTTCTCTCTGTCATTTGAATTGGAGGGAAAGGGAAACCCTTTTGAATAACAATTCTTTTCTTTTTTTTTTAATACTTTCTAAATGTATTATGATTAGTTTAAATCTTTAATAAAGAAAGTAAGAAAGTGGGTAACAAATTTTGTTTTTTTGGAACTTGGGTTTTGTTTTAGTTTAATCATGTAGATTTATTTTTGAATATTTGTTATGTGTTCAAAGGGTTAATGATGGAGAATTCTGAGGGGTCAAGTCAATGTGATGGTAAAGTTTTTAGATGGGTCTGCTAAAAGTGAGAGAGTGAAAGAGTCAGTTGAGGGAAAGTGGGGT

Coding sequence (CDS)

ATGGGTCCTGATTCCGATTTCAAATCCCATTTGGCCACTGAAATTAGCTCCTTCTCCTCCCGTACTGTCTCTTGCTCTCACCGCCATTTCTCCCTCCCTTTCATCGATTGGTATCGCCTTTTTGGGGTCCAACAAGACGCTGGAATCGATTCCATTCGGAGGCGATATCTCAAGCTTGCTCTGCAACTTCATCCAGACAAAAACTCGCACCCCAAGGCCGAAATCGCCTTCAAGCTTGTCTCTGAGGGATATGGTTGTTTGAGTGATAATGTGAAGAGAAGGGAATTTGATTTGGATAGGAAGAAGAAGTTTTGCCCTGATTGCAACACAATCCCTTATAGAAATTTCACCTCTGCTAGAAACTTGAATGTACTAGGAGGTTTTCACAACAACAACAACATCAACAACATTCATGGAGATTTGAGAGATATGAAAGAGAGATTAAGAGAGGAAGCTAATGTGATTAAGAATTGTTTGAGGACTAATAACTCTGCAGCAAACAGAGAAACTTCACTATTCAATCCACCTGGTTTCACCATGTTTCATTACAAAAACCACAAAGAAACCCCAATCTTCAATCCTTCTGATTATGTTTGTCAAGGCTACCCACATTTCCGAACCCGAATCCACCCGAAACCGCAGAGTTTTCGGTGTTTTAGAACTGGAAATGCATTGAAGTATGAACAAGGTAGAGGGAAGTATGAGTATCCGGTGTTTGAAATTAGTAGATCAGAAAGTTTTCTTTTACAGAAACAATCTGCTTTTGTTTATTCAAATGTTAACAAACCACATTGGTGA

Protein sequence

MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSARNLNVLGGFHNNNNINNIHGDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPPGFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEYPVFEISRSESFLLQKQSAFVYSNVNKPHW
Homology
BLAST of Bhi01G000752 vs. TAIR 10
Match: AT3G06778.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 160.2 bits (404), Expect = 2.2e-39
Identity = 86/211 (40.76%), Postives = 128/211 (60.66%), Query Frame = 0

Query: 3   PDSDFKSHLATEISSFSSRTVSCSHRH---FSLPFIDWYRLFGVQQDAGIDSIRRRYLKL 62
           P  D K+ L ++I + S   +SC H +       FIDWY + G+Q+DA +  IR+RY KL
Sbjct: 6   PGPDPKAALVSDILALSRSPISCIHINRISSGSCFIDWYLILGIQEDAEVKVIRKRYHKL 65

Query: 63  ALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSA 122
           AL++HPDKN+HPKA+IAFKL+ E Y CLSD  KRR F++DR+   C  C+ + ++   S 
Sbjct: 66  ALKVHPDKNNHPKADIAFKLIHEAYLCLSDETKRRSFNIDRRNNICLKCSRVSHK---SK 125

Query: 123 RNLNVLGGFHNNNNINNIHGDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPPGFT 182
            N        N++  N     L+D++++ REE  VI+ CL+TN++      +   PP + 
Sbjct: 126 EN-------RNDSKPNRFCQTLKDIRDKFREENMVIERCLKTNSAFFMGNRTNETPPVYG 185

Query: 183 M-FHYKNHKETPIFNPSDYVCQGYPHFRTRI 210
           +    + +KE+P+FNPSDY   GYPH R R+
Sbjct: 186 IPKQNRINKESPVFNPSDYRLWGYPHVRNRV 206

BLAST of Bhi01G000752 vs. TAIR 10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 73.2 bits (178), Expect = 3.6e-13
Identity = 30/62 (48.39%), Postives = 45/62 (72.58%), Query Frame = 0

Query: 36  DWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
           D+Y + G++++  +D IR+ Y KL+L++HPDKN  P +E AFK VS+ + CLSD   RR+
Sbjct: 99  DYYAILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQ 158

Query: 96  FD 98
           FD
Sbjct: 159 FD 160

BLAST of Bhi01G000752 vs. TAIR 10
Match: AT3G04980.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 72.0 bits (175), Expect = 8.0e-13
Identity = 44/107 (41.12%), Postives = 56/107 (52.34%), Query Frame = 0

Query: 36  DWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
           DWY +  VQ  A  D+I+++Y KLAL LHPDKN    AE AFKLV E    LSD +KR +
Sbjct: 48  DWYGVLQVQPYADADTIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSDQIKRSQ 107

Query: 96  FDLDRKKKFCPDCNTIPYRNFTSARNLNVLGGFH---NNNNINNIHG 140
           +D           N     +  + R++NV  G H    NN   NI G
Sbjct: 108 YD-----------NRYRSHSMFANRHVNVYSGRHCAATNNAAENIAG 143

BLAST of Bhi01G000752 vs. TAIR 10
Match: AT5G18750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.4e-12
Identity = 37/82 (45.12%), Postives = 48/82 (58.54%), Query Frame = 0

Query: 23  VSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSE 82
           V C+    S    DWY++  V+Q A  ++I+++Y KLAL LHPDKN  P AE AFK + E
Sbjct: 53  VHCAALEKSGDETDWYKILQVEQTADENTIKKQYKKLALHLHPDKNKLPGAESAFKTIGE 112

Query: 83  GYGCLSDNVKRREFDLDRKKKF 105
               L D  KRR  D+ RK  F
Sbjct: 113 AQRVLLDKDKRRFHDMRRKPVF 134

BLAST of Bhi01G000752 vs. TAIR 10
Match: AT4G19570.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 4.0e-12
Identity = 33/66 (50.00%), Postives = 43/66 (65.15%), Query Frame = 0

Query: 36  DWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
           DWYR+ GV   A  +++++RY KLAL LHPDKN    AE AFKL+ E +  LSD  +R  
Sbjct: 66  DWYRVLGVDPLADDEAVKKRYRKLALLLHPDKNRFTGAEGAFKLILEAWDLLSDKSQRSS 125

Query: 96  FDLDRK 102
           +D  RK
Sbjct: 126 YDQKRK 131

BLAST of Bhi01G000752 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 5.1e-12
Identity = 30/62 (48.39%), Postives = 45/62 (72.58%), Query Frame = 0

Query: 36  DWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
           D+Y + G++++  +D IR+ Y KL+L++HPDKN  P +E AFK VS+ + CLSD   RR+
Sbjct: 99  DYYAILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQ 158

Query: 96  FD 98
           FD
Sbjct: 159 FD 160

BLAST of Bhi01G000752 vs. ExPASy Swiss-Prot
Match: Q8TQR1 (Chaperone protein DnaJ OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.5e-11
Identity = 30/62 (48.39%), Postives = 42/62 (67.74%), Query Frame = 0

Query: 36 DWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
          D+Y + G+ +DA ++ I++ Y KLALQ HPD+N  P AE  FK +SE Y  LSD  KR +
Sbjct: 6  DYYEILGLPKDASVEDIKKTYRKLALQYHPDRNKDPGAEDKFKEISEAYAVLSDTEKRAQ 65

Query: 96 FD 98
          +D
Sbjct: 66 YD 67

BLAST of Bhi01G000752 vs. ExPASy Swiss-Prot
Match: P0CW07 (Chaperone protein DnaJ OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-11
Identity = 29/62 (46.77%), Postives = 42/62 (67.74%), Query Frame = 0

Query: 36 DWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
          D+Y + G+ +D+ ++ I++ Y KLALQ HPD+N  P AE  FK +SE Y  LSD  KR +
Sbjct: 6  DYYEILGLSKDSSVEDIKKTYRKLALQYHPDRNKEPGAEEKFKEISEAYAVLSDAEKRAQ 65

Query: 96 FD 98
          +D
Sbjct: 66 YD 67

BLAST of Bhi01G000752 vs. ExPASy Swiss-Prot
Match: P0CW06 (Chaperone protein DnaJ OS=Methanosarcina mazei OX=2209 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-11
Identity = 29/62 (46.77%), Postives = 42/62 (67.74%), Query Frame = 0

Query: 36 DWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
          D+Y + G+ +D+ ++ I++ Y KLALQ HPD+N  P AE  FK +SE Y  LSD  KR +
Sbjct: 6  DYYEILGLSKDSSVEDIKKTYRKLALQYHPDRNKEPGAEEKFKEISEAYAVLSDAEKRAQ 65

Query: 96 FD 98
          +D
Sbjct: 66 YD 67

BLAST of Bhi01G000752 vs. ExPASy Swiss-Prot
Match: Q24133 (DnaJ protein homolog 1 OS=Drosophila melanogaster OX=7227 GN=DnaJ-1 PE=1 SV=3)

HSP 1 Score: 67.4 bits (163), Expect = 2.8e-10
Identity = 30/62 (48.39%), Postives = 42/62 (67.74%), Query Frame = 0

Query: 36 DWYRLFGVQQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
          D+Y++ G+++ A  D I++ Y KLAL+ HPDKN  P+AE  FK ++E Y  LSD  KR  
Sbjct: 4  DFYKILGLERKASDDEIKKAYRKLALKYHPDKNKSPQAEERFKEIAEAYEVLSDKKKRDI 63

Query: 96 FD 98
          FD
Sbjct: 64 FD 65

BLAST of Bhi01G000752 vs. NCBI nr
Match: XP_038902437.1 (uncharacterized protein LOC120089081 [Benincasa hispida])

HSP 1 Score: 559.3 bits (1440), Expect = 1.8e-155
Identity = 265/265 (100.00%), Postives = 265/265 (100.00%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA
Sbjct: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120

Query: 121 NLNVLGGFHNNNNINNIHGDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPPGFTM 180
           NLNVLGGFHNNNNINNIHGDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPPGFTM
Sbjct: 121 NLNVLGGFHNNNNINNIHGDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPPGFTM 180

Query: 181 FHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEYPVFE 240
           FHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEYPVFE
Sbjct: 181 FHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEYPVFE 240

Query: 241 ISRSESFLLQKQSAFVYSNVNKPHW 266
           ISRSESFLLQKQSAFVYSNVNKPHW
Sbjct: 241 ISRSESFLLQKQSAFVYSNVNKPHW 265

BLAST of Bhi01G000752 vs. NCBI nr
Match: TYK16200.1 (chaperone protein DnaJ [Cucumis melo var. makuwa])

HSP 1 Score: 468.0 bits (1203), Expect = 5.4e-128
Identity = 233/271 (85.98%), Postives = 242/271 (89.30%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLA EISSFSSRTVSC HRHFS PFIDWYRLFGVQQDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLAYEISSFSSRTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNFTSA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNVL---GGFHNNNNINNIH-GDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPP 180
           NLN L   GGFH     NNIH G LRDMKERLREEANVI+NCLR NNS++  ETSLFNPP
Sbjct: 121 NLNALNIFGGFH-----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPP 180

Query: 181 GFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEY 240
           G  MFH++N+KETPIFNPSDYVCQGYPHFRTRIHPK +SFRCFR+GNALKYEQGRGKYEY
Sbjct: 181 GCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEY 240

Query: 241 PVFEI-SRSESFLLQKQSAFVYSNVNK-PHW 266
           PVFEI S+SESFLLQKQSAFVYSNVNK  HW
Sbjct: 241 PVFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

BLAST of Bhi01G000752 vs. NCBI nr
Match: KAA0049521.1 (chaperone protein DnaJ [Cucumis melo var. makuwa])

HSP 1 Score: 466.5 bits (1199), Expect = 1.6e-127
Identity = 232/271 (85.61%), Postives = 241/271 (88.93%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHL  EISSFSSRTVSC HRHFS PFIDWYRLFGVQQDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLTYEISSFSSRTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNFTSA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNVL---GGFHNNNNINNIH-GDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPP 180
           NLN L   GGFH     NNIH G LRDMKERLREEANVI+NCLR NNS++  ETSLFNPP
Sbjct: 121 NLNALNIFGGFH-----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPP 180

Query: 181 GFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEY 240
           G  MFH++N+KETPIFNPSDYVCQGYPHFRTRIHPK +SFRCFR+GNALKYEQGRGKYEY
Sbjct: 181 GCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEY 240

Query: 241 PVFEI-SRSESFLLQKQSAFVYSNVNK-PHW 266
           PVFEI S+SESFLLQKQSAFVYSNVNK  HW
Sbjct: 241 PVFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

BLAST of Bhi01G000752 vs. NCBI nr
Match: XP_008438915.1 (PREDICTED: chaperone protein DnaJ [Cucumis melo])

HSP 1 Score: 466.1 bits (1198), Expect = 2.0e-127
Identity = 233/272 (85.66%), Postives = 241/272 (88.60%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLA EISSFSS TVSC HRHFS PFIDWYRLFGVQQDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLAYEISSFSSLTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNFTSA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNVL---GGFHNNNNINNIH-GDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPP 180
           NLN L   GGFH     NNIH G LRDMKERLREEANVI+NCLR NNS++  ETSLFNPP
Sbjct: 121 NLNALNIFGGFH-----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPP 180

Query: 181 GFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEY 240
           G  MFH++N+KETPIFNPSDYVCQGYPHFRTRIHPK +SFRCFR+GNALKYEQGRGKYEY
Sbjct: 181 GCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEY 240

Query: 241 PVFEI--SRSESFLLQKQSAFVYSNVNK-PHW 266
           PVFEI  SRSESFLLQKQSAFVYSNVNK  HW
Sbjct: 241 PVFEISSSRSESFLLQKQSAFVYSNVNKSSHW 267

BLAST of Bhi01G000752 vs. NCBI nr
Match: XP_004134496.2 (uncharacterized protein LOC101213407 [Cucumis sativus] >KGN57140.1 hypothetical protein Csa_010349 [Cucumis sativus])

HSP 1 Score: 451.4 bits (1160), Expect = 5.2e-123
Identity = 228/272 (83.82%), Postives = 238/272 (87.50%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           MGP SDFKSHLA+EISSFSS TVSC HR FS PFIDWYRLFGVQQDA ID IR RYLKLA
Sbjct: 1   MGPHSDFKSHLASEISSFSSLTVSCPHRLFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFC DCNTIPYRN+TSA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKDKFCADCNTIPYRNYTSAT 120

Query: 121 N---LNVLGGFHNNNNINNIHG-DLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPP 180
           N   LNVLG FH     NNIHG  LRD+KERLREEANVI+ CLR NNS+   ETSLFNPP
Sbjct: 121 NLNALNVLGSFH-----NNIHGRGLRDVKERLREEANVIEYCLRANNSSTTTETSLFNPP 180

Query: 181 GFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEY 240
           G +MFH++N+KETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFR+GNALKYEQGRGK EY
Sbjct: 181 GCSMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRSGNALKYEQGRGKCEY 240

Query: 241 PVFEIS--RSESFLLQKQSAFVYSNVNK-PHW 266
           PVFEIS  RSESFLLQKQSAFVYSNVNK  HW
Sbjct: 241 PVFEISSNRSESFLLQKQSAFVYSNVNKSSHW 267

BLAST of Bhi01G000752 vs. ExPASy TrEMBL
Match: A0A5D3CXW5 (Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001280 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.6e-128
Identity = 233/271 (85.98%), Postives = 242/271 (89.30%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLA EISSFSSRTVSC HRHFS PFIDWYRLFGVQQDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLAYEISSFSSRTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNFTSA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNVL---GGFHNNNNINNIH-GDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPP 180
           NLN L   GGFH     NNIH G LRDMKERLREEANVI+NCLR NNS++  ETSLFNPP
Sbjct: 121 NLNALNIFGGFH-----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPP 180

Query: 181 GFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEY 240
           G  MFH++N+KETPIFNPSDYVCQGYPHFRTRIHPK +SFRCFR+GNALKYEQGRGKYEY
Sbjct: 181 GCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEY 240

Query: 241 PVFEI-SRSESFLLQKQSAFVYSNVNK-PHW 266
           PVFEI S+SESFLLQKQSAFVYSNVNK  HW
Sbjct: 241 PVFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

BLAST of Bhi01G000752 vs. ExPASy TrEMBL
Match: A0A5A7U7H9 (Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G007750 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 7.6e-128
Identity = 232/271 (85.61%), Postives = 241/271 (88.93%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHL  EISSFSSRTVSC HRHFS PFIDWYRLFGVQQDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLTYEISSFSSRTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNFTSA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNVL---GGFHNNNNINNIH-GDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPP 180
           NLN L   GGFH     NNIH G LRDMKERLREEANVI+NCLR NNS++  ETSLFNPP
Sbjct: 121 NLNALNIFGGFH-----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPP 180

Query: 181 GFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEY 240
           G  MFH++N+KETPIFNPSDYVCQGYPHFRTRIHPK +SFRCFR+GNALKYEQGRGKYEY
Sbjct: 181 GCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEY 240

Query: 241 PVFEI-SRSESFLLQKQSAFVYSNVNK-PHW 266
           PVFEI S+SESFLLQKQSAFVYSNVNK  HW
Sbjct: 241 PVFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

BLAST of Bhi01G000752 vs. ExPASy TrEMBL
Match: A0A1S3AXH7 (chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103483872 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 9.9e-128
Identity = 233/272 (85.66%), Postives = 241/272 (88.60%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLA EISSFSS TVSC HRHFS PFIDWYRLFGVQQDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLAYEISSFSSLTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNFTSA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNVL---GGFHNNNNINNIH-GDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPP 180
           NLN L   GGFH     NNIH G LRDMKERLREEANVI+NCLR NNS++  ETSLFNPP
Sbjct: 121 NLNALNIFGGFH-----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPP 180

Query: 181 GFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEY 240
           G  MFH++N+KETPIFNPSDYVCQGYPHFRTRIHPK +SFRCFR+GNALKYEQGRGKYEY
Sbjct: 181 GCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEY 240

Query: 241 PVFEI--SRSESFLLQKQSAFVYSNVNK-PHW 266
           PVFEI  SRSESFLLQKQSAFVYSNVNK  HW
Sbjct: 241 PVFEISSSRSESFLLQKQSAFVYSNVNKSSHW 267

BLAST of Bhi01G000752 vs. ExPASy TrEMBL
Match: A0A0A0L5B2 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165080 PE=4 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 2.5e-123
Identity = 228/272 (83.82%), Postives = 238/272 (87.50%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           MGP SDFKSHLA+EISSFSS TVSC HR FS PFIDWYRLFGVQQDA ID IR RYLKLA
Sbjct: 1   MGPHSDFKSHLASEISSFSSLTVSCPHRLFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFC DCNTIPYRN+TSA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKDKFCADCNTIPYRNYTSAT 120

Query: 121 N---LNVLGGFHNNNNINNIHG-DLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPP 180
           N   LNVLG FH     NNIHG  LRD+KERLREEANVI+ CLR NNS+   ETSLFNPP
Sbjct: 121 NLNALNVLGSFH-----NNIHGRGLRDVKERLREEANVIEYCLRANNSSTTTETSLFNPP 180

Query: 181 GFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEY 240
           G +MFH++N+KETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFR+GNALKYEQGRGK EY
Sbjct: 181 GCSMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRSGNALKYEQGRGKCEY 240

Query: 241 PVFEIS--RSESFLLQKQSAFVYSNVNK-PHW 266
           PVFEIS  RSESFLLQKQSAFVYSNVNK  HW
Sbjct: 241 PVFEISSNRSESFLLQKQSAFVYSNVNKSSHW 267

BLAST of Bhi01G000752 vs. ExPASy TrEMBL
Match: A0A6J1IXP0 (dnaJ homolog subfamily B member 9-like OS=Cucurbita maxima OX=3661 GN=LOC111479463 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.2e-103
Identity = 192/271 (70.85%), Postives = 210/271 (77.49%), Query Frame = 0

Query: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60
           M PDSDFKS LA EI SFS+R+VSC HRHFS PF+DWYRLFGV+QDAGIDSIRRRYLKLA
Sbjct: 1   MAPDSDFKSDLAAEICSFSARSVSCPHRHFSRPFLDWYRLFGVEQDAGIDSIRRRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120
           LQLHPDKN+HPKAEIAFKLVSEGYGCL DNVKRR FDLDR KKFCPDCNTIPYRN +SAR
Sbjct: 61  LQLHPDKNAHPKAEIAFKLVSEGYGCLIDNVKRRAFDLDRMKKFCPDCNTIPYRNLSSAR 120

Query: 121 NLNVLGGFHNNNNINNIHGDLRDMKERLREEANVIKNCLRTNNSAANRETS--LFNPPGF 180
           N N L                           N+ +NCLR NNS   RE S    N P  
Sbjct: 121 NSNPL---------------------------NLFENCLRANNS---REFSQQFNNSPDC 180

Query: 181 TM----FHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKY 240
            +    FH++NHKE+PIFNPSDY CQGYPHFRT+IHPKPQS+RCFRTGN+LKYEQGRGKY
Sbjct: 181 LINNGRFHHRNHKESPIFNPSDYACQGYPHFRTQIHPKPQSYRCFRTGNSLKYEQGRGKY 240

Query: 241 EYPVFEISRSESFLLQKQSAFVYSNVNKPHW 266
           EYPVFE+SRSE F+LQ+QSAFV SNVNK HW
Sbjct: 241 EYPVFEMSRSERFVLQQQSAFVCSNVNKSHW 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G06778.12.2e-3940.76Chaperone DnaJ-domain superfamily protein [more]
AT5G49060.13.6e-1348.39Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT3G04980.18.0e-1341.12DNAJ heat shock N-terminal domain-containing protein [more]
AT5G18750.11.4e-1245.12DNAJ heat shock N-terminal domain-containing protein [more]
AT4G19570.14.0e-1250.00Chaperone DnaJ-domain superfamily protein [more]
Match NameE-valueIdentityDescription
Q9FH285.1e-1248.39Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2[more]
Q8TQR11.5e-1148.39Chaperone protein DnaJ OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 28... [more]
P0CW073.3e-1146.77Chaperone protein DnaJ OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 /... [more]
P0CW063.3e-1146.77Chaperone protein DnaJ OS=Methanosarcina mazei OX=2209 GN=dnaJ PE=3 SV=1[more]
Q241332.8e-1048.39DnaJ protein homolog 1 OS=Drosophila melanogaster OX=7227 GN=DnaJ-1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
XP_038902437.11.8e-155100.00uncharacterized protein LOC120089081 [Benincasa hispida][more]
TYK16200.15.4e-12885.98chaperone protein DnaJ [Cucumis melo var. makuwa][more]
KAA0049521.11.6e-12785.61chaperone protein DnaJ [Cucumis melo var. makuwa][more]
XP_008438915.12.0e-12785.66PREDICTED: chaperone protein DnaJ [Cucumis melo][more]
XP_004134496.25.2e-12383.82uncharacterized protein LOC101213407 [Cucumis sativus] >KGN57140.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
A0A5D3CXW52.6e-12885.98Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A5A7U7H97.6e-12885.61Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3AXH79.9e-12885.66chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103483872 PE=4 SV=1[more]
A0A0A0L5B22.5e-12383.82J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165080 PE=4 SV=1[more]
A0A6J1IXP02.2e-10370.85dnaJ homolog subfamily B member 9-like OS=Cucurbita maxima OX=3661 GN=LOC1114794... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 131..151
NoneNo IPR availablePANTHERPTHR44137:SF13CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEINcoord: 4..248
NoneNo IPR availablePANTHERPTHR44137BNAC03G44070D PROTEINcoord: 4..248
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 72..92
score: 42.4
coord: 56..71
score: 50.47
coord: 38..56
score: 32.6
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 35..92
e-value: 1.0E-15
score: 68.2
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 36..97
e-value: 1.3E-17
score: 63.6
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 36..100
score: 15.443152
IPR001623DnaJ domainCDDcd06257DnaJcoord: 36..89
e-value: 3.22694E-16
score: 69.1113
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 35..152
e-value: 8.5E-20
score: 73.3
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 24..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000752Bhi01M000752mRNA