Bhi01G000682 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000682
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionaspartic proteinase CDR1-like
Locationchr1: 17552634 .. 17553914 (-)
RNA-Seq ExpressionBhi01G000682
SyntenyBhi01G000682
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAGGCATACGGTACCGACCGAAGTTGGCTTCACCGCACGTTTGATTCATCGAGATTCACCTCTATCACCATTTTACGATCACGCCATGACAGACACTGCACGGATAGAGGCGACCGTTCATCGTTCTAGGTCCCGGCTGAATTATCTGTATTACAACATGTTATCAGAAAATACATTAGACAATGATGTGTCACTCTCACCAACATTAGTTAATGAAGGTGGTGAGTACCTTATAAGTTTCAACATTGGAAATCCTCCAAGTCAAGTGATGGGGTTTGCAGACACATCAAATGGTCTCATTTGGGTGCAATGCTCAGACTGCAATAACCAATGTGAGCCAAAAAAAGAAGGCCCCACCACCAAGTTCCTCTCTTCCAAATCTTTCACTTATGAGATGGAGCCATGTGGCTCTAACTTTTGCAATTCCTTAACTGGCTTCCAGACCTGCAATTCATCTGACAAATGGTGCAAATATAGATTAGTGTATGCAGATAATAAAGCAACAAGTGGAATTCTTTCATCTGATAGTTTTAGTTTTGATACCTCAGATGGGAAGCTTGTGGATGTTGGCTATTTGAACTTTGGCTGCTCAGAAGCTCCTTCAACAGAAGATATACAGAGTTATACAGGGAGTGTGGGCTTGAACCAAACACCCTTGTCATTAATTTCTCAATTGGGTATCAAAAAGTTCTCCTACTGTTTGGTTCCTTTCAATAATTTAGGATCAACAAGTAAAATGTATTTTGGATCATTACCTGTGACTTCTGGGAGTCAAACTCCATTGTTATACCCCGATTCAGATGCTTATTACGTGAAGGTTCTAGGAATCGGCCTCAGCAATGATGAGCCCCAGTTAGATAGAGTTTTTGATGTATACGATGTCAGAGATGGGTGGATTATAGATTCAGGAACAACATACTCAAGTCTTGAAACAGATACATTTGATAGTTTGCTAGCTAAATTCCTTACACTACCAGATTTACCACAGGAAAAAAAAGACCCTAGAGACAGATTCGAGTTGTGCTTTGCAGCAAATGCAAATGATCTGGAGTCATTTCCAGATGTTACGGTTCATTTTGATGGTGCAGATTTAATTCTTAACGTAGAAAGTACCTTTGTGAAGATAGAGGACGATGGAATTTTCTGTCTTGCCTTTCTGCGTTCTGGATCTCCAGTTTCTATATTAGGGAACTTTCAGCTGCAAAACTTCCATGTTGGATATGACCTTGAAGCTCAAGTTATTTCCTTTGCTCCTGTTGACTGTGCTGATTCCTAG

mRNA sequence

ATGGCAAGGCATACGGTACCGACCGAAGTTGGCTTCACCGCACGTTTGATTCATCGAGATTCACCTCTATCACCATTTTACGATCACGCCATGACAGACACTGCACGGATAGAGGCGACCGTTCATCGTTCTAGGTCCCGGCTGAATTATCTGTATTACAACATGTTATCAGAAAATACATTAGACAATGATGTGTCACTCTCACCAACATTAGTTAATGAAGGTGGTGAGTACCTTATAAGTTTCAACATTGGAAATCCTCCAAGTCAAGTGATGGGGTTTGCAGACACATCAAATGGTCTCATTTGGGTGCAATGCTCAGACTGCAATAACCAATGTGAGCCAAAAAAAGAAGGCCCCACCACCAAGTTCCTCTCTTCCAAATCTTTCACTTATGAGATGGAGCCATGTGGCTCTAACTTTTGCAATTCCTTAACTGGCTTCCAGACCTGCAATTCATCTGACAAATGGTGCAAATATAGATTAGTGTATGCAGATAATAAAGCAACAAGTGGAATTCTTTCATCTGATAGTTTTAGTTTTGATACCTCAGATGGGAAGCTTGTGGATGTTGGCTATTTGAACTTTGGCTGCTCAGAAGCTCCTTCAACAGAAGATATACAGAGTTATACAGGGAGTGTGGGCTTGAACCAAACACCCTTGTCATTAATTTCTCAATTGGGTATCAAAAAGTTCTCCTACTGTTTGGTTCCTTTCAATAATTTAGGATCAACAAGTAAAATGTATTTTGGATCATTACCTGTGACTTCTGGGAGTCAAACTCCATTGTTATACCCCGATTCAGATGCTTATTACGTGAAGGTTCTAGGAATCGGCCTCAGCAATGATGAGCCCCAGTTAGATAGAGTTTTTGATGTATACGATGTCAGAGATGGGTGGATTATAGATTCAGGAACAACATACTCAAGTCTTGAAACAGATACATTTGATAGTTTGCTAGCTAAATTCCTTACACTACCAGATTTACCACAGGAAAAAAAAGACCCTAGAGACAGATTCGAGTTGTGCTTTGCAGCAAATGCAAATGATCTGGAGTCATTTCCAGATGTTACGGTTCATTTTGATGGTGCAGATTTAATTCTTAACGTAGAAAGTACCTTTGTGAAGATAGAGGACGATGGAATTTTCTGTCTTGCCTTTCTGCGTTCTGGATCTCCAGTTTCTATATTAGGGAACTTTCAGCTGCAAAACTTCCATGTTGGATATGACCTTGAAGCTCAAGTTATTTCCTTTGCTCCTGTTGACTGTGCTGATTCCTAG

Coding sequence (CDS)

ATGGCAAGGCATACGGTACCGACCGAAGTTGGCTTCACCGCACGTTTGATTCATCGAGATTCACCTCTATCACCATTTTACGATCACGCCATGACAGACACTGCACGGATAGAGGCGACCGTTCATCGTTCTAGGTCCCGGCTGAATTATCTGTATTACAACATGTTATCAGAAAATACATTAGACAATGATGTGTCACTCTCACCAACATTAGTTAATGAAGGTGGTGAGTACCTTATAAGTTTCAACATTGGAAATCCTCCAAGTCAAGTGATGGGGTTTGCAGACACATCAAATGGTCTCATTTGGGTGCAATGCTCAGACTGCAATAACCAATGTGAGCCAAAAAAAGAAGGCCCCACCACCAAGTTCCTCTCTTCCAAATCTTTCACTTATGAGATGGAGCCATGTGGCTCTAACTTTTGCAATTCCTTAACTGGCTTCCAGACCTGCAATTCATCTGACAAATGGTGCAAATATAGATTAGTGTATGCAGATAATAAAGCAACAAGTGGAATTCTTTCATCTGATAGTTTTAGTTTTGATACCTCAGATGGGAAGCTTGTGGATGTTGGCTATTTGAACTTTGGCTGCTCAGAAGCTCCTTCAACAGAAGATATACAGAGTTATACAGGGAGTGTGGGCTTGAACCAAACACCCTTGTCATTAATTTCTCAATTGGGTATCAAAAAGTTCTCCTACTGTTTGGTTCCTTTCAATAATTTAGGATCAACAAGTAAAATGTATTTTGGATCATTACCTGTGACTTCTGGGAGTCAAACTCCATTGTTATACCCCGATTCAGATGCTTATTACGTGAAGGTTCTAGGAATCGGCCTCAGCAATGATGAGCCCCAGTTAGATAGAGTTTTTGATGTATACGATGTCAGAGATGGGTGGATTATAGATTCAGGAACAACATACTCAAGTCTTGAAACAGATACATTTGATAGTTTGCTAGCTAAATTCCTTACACTACCAGATTTACCACAGGAAAAAAAAGACCCTAGAGACAGATTCGAGTTGTGCTTTGCAGCAAATGCAAATGATCTGGAGTCATTTCCAGATGTTACGGTTCATTTTGATGGTGCAGATTTAATTCTTAACGTAGAAAGTACCTTTGTGAAGATAGAGGACGATGGAATTTTCTGTCTTGCCTTTCTGCGTTCTGGATCTCCAGTTTCTATATTAGGGAACTTTCAGCTGCAAAACTTCCATGTTGGATATGACCTTGAAGCTCAAGTTATTTCCTTTGCTCCTGTTGACTGTGCTGATTCCTAG

Protein sequence

MARHTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTLDNDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNNLGSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWIIDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLESFPDVTVHFDGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPVDCADS
Homology
BLAST of Bhi01G000682 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 238.8 bits (608), Expect = 8.0e-63
Identity = 154/428 (35.98%), Postives = 230/428 (53.74%), Query Frame = 0

Query: 9   EVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTLDNDVSLS 68
           ++GFTA LIHRDSP SPFY+   T + R+   +HRS   +N +++    +NT    + L+
Sbjct: 28  KLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRS---VNRVFHFTEKDNTPQPQIDLT 87

Query: 69  PTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCS---DCNNQCEPKKEGPTTKFL 128
               +  GEYL++ +IG PP  +M  ADT + L+W QC+   DC  Q +P  +  T    
Sbjct: 88  ----SNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKT---- 147

Query: 129 SSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSD 188
              S TY+   C S+ C +L    +C+++D  C Y L Y DN  T G ++ D+ +  +SD
Sbjct: 148 ---SSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSD 207

Query: 189 GKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIK---KFSYCLVPF-NN 248
            + + +  +  GC    +    +  +G VGL   P+SLI QLG     KFSYCLVP  + 
Sbjct: 208 TRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTSK 267

Query: 249 LGSTSKMYFGSLPVTSGS---QTPLLYPDSDA--YYVKVLGIGLSNDEPQLDRVFDVYDV 308
              TSK+ FG+  + SGS    TPL+   S    YY+ +  I + + + Q     D    
Sbjct: 268 KDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSG-SDSESS 327

Query: 309 RDGWIIDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLESFPD 368
               IIDSGTT + L T+ +  L     +  D  ++K+DP+    LC++A   DL+  P 
Sbjct: 328 EGNIIIDSGTTLTLLPTEFYSELEDAVASSID-AEKKQDPQSGLSLCYSA-TGDLK-VPV 387

Query: 369 VTVHFDGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVI 425
           +T+HFDGAD+ L+  + FV++ +D + C AF R     SI GN    NF VGYD  ++ +
Sbjct: 388 ITMHFDGADVKLDSSNAFVQVSED-LVCFAF-RGSPSFSIYGNVAQMNFLVGYDTVSKTV 435

BLAST of Bhi01G000682 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 213.0 bits (541), Expect = 4.7e-55
Identity = 154/437 (35.24%), Postives = 223/437 (51.03%), Query Frame = 0

Query: 12  FTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTLDNDVSLSPTL 71
           F+  LIHRDSPLSP Y+  +T T R+ A   RS SR +  + + LS+  L +       L
Sbjct: 26  FSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSR-SRRFNHQLSQTDLQSG------L 85

Query: 72  VNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTTKFLSSKSFT 131
           +   GE+ +S  IG PP +V   ADT + L WVQC  C  QC  K+ GP   F   KS T
Sbjct: 86  IGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPC-QQCY-KENGPI--FDKKKSST 145

Query: 132 YEMEPCGSNFCNSLTGFQT-CNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSDGKLVD 191
           Y+ EPC S  C +L+  +  C+ S+  CKYR  Y D   + G +++++ S D++ G  V 
Sbjct: 146 YKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVS 205

Query: 192 VGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLG---IKKFSYCL---------VP 251
                FGC         ++ +G +GL    LSLISQLG    KKFSYCL           
Sbjct: 206 FPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLSHKSATTNGTS 265

Query: 252 FNNLGSTSKMYFGSLPVTSG-SQTPLLYPDSDAYY---VKVLGIGL-------SNDEPQL 311
             NLG+ S     SL   SG   TPL+  +   YY   ++ + +G        S+  P  
Sbjct: 266 VINLGTNS--IPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKIPYTGSSYNPND 325

Query: 312 DRVFDVYDVRDGWIIDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAAN 371
           D +    +     IIDSGTT + LE   FD   +         +   DP+     CF + 
Sbjct: 326 DGILS--ETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLSHCFKSG 385

Query: 372 ANDLESFPDVTVHFDGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHV 425
           + ++   P++TVHF GAD+ L+  + FVK+ +D + CL+ + + + V+I GNF   +F V
Sbjct: 386 SAEI-GLPEITVHFTGADVRLSPINAFVKLSED-MVCLSMVPT-TEVAIYGNFAQMDFLV 444

BLAST of Bhi01G000682 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.3e-52
Identity = 145/431 (33.64%), Postives = 207/431 (48.03%), Query Frame = 0

Query: 13  TARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTLDNDVSLSPTLV 72
           T  LIHRDSP SP Y+   T + R+ A   RS SR                   L   L+
Sbjct: 30  TVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISR----------SRRFTTKTDLQSGLI 89

Query: 73  NEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTTKFLSSKSFTY 132
           + GGEY +S +IG PPS+V   ADT + L WVQC  C  QC  K+  P   F   KS TY
Sbjct: 90  SNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPC-QQCY-KQNSPL--FDKKKSSTY 149

Query: 133 EMEPCGSNFCNSLTGFQT-CNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSDGKLVDV 192
           + E C S  C +L+  +  C+ S   CKYR  Y DN  T G +++++ S D+S G  V  
Sbjct: 150 KTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKGDVATETISIDSSSGSSVSF 209

Query: 193 GYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGI---KKFSYCL---VPFNNLGST 252
               FGC         ++ +G +GL   PLSL+SQLG    KKFSYCL       N  S 
Sbjct: 210 PGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLGSSIGKKFSYCLSHTAATTNGTSV 269

Query: 253 SKMYFGSLPVTSGSQ-----TPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVR--- 312
             +   S+P           TPL+  D + YY   L   ++  + +L      Y +    
Sbjct: 270 INLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTLE-AVTVGKTKLPYTGGGYGLNGKS 329

Query: 313 ----DGWIIDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLES 372
                  IIDSGTT + L++  +D             +   DP+     CF +   ++  
Sbjct: 330 SKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQGLLTHCFKSGDKEI-G 389

Query: 373 FPDVTVHFDGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEA 425
            P +T+HF  AD+ L+  + FVK+ +D + CL+ + + + V+I GN    +F VGYDLE 
Sbjct: 390 LPAITMHFTNADVKLSPINAFVKLNEDTV-CLSMIPT-TEVAIYGNMVQMDFLVGYDLET 442

BLAST of Bhi01G000682 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.8e-51
Identity = 141/428 (32.94%), Postives = 214/428 (50.00%), Query Frame = 0

Query: 11  GFTARLIHRDSPLSPFYDHAMTDTARIEATVHRS-RSRLNYLYYNMLSENTLDNDVSLSP 70
           GFT  LIHRDSP SPFY+ A T + R+   + RS RS L +        N   +  S   
Sbjct: 25  GFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRSARSTLQF-------SNDDASPNSPQS 84

Query: 71  TLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTTKFLSSKS 130
            + +  GEYL++ +IG PP  ++  ADT + LIW QC    N CE   +  +  F   +S
Sbjct: 85  FITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQC----NPCEDCYQQTSPLFDPKES 144

Query: 131 FTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSDGKLV 190
            TY    C S+ C +L    +C++ +  C Y + Y DN  T G ++ D+ +  +S  + V
Sbjct: 145 STYRKVSCSSSQCRALED-ASCSTDENTCSYTITYGDNSYTKGDVAVDTVTMGSSGRRPV 204

Query: 191 DVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIK---KFSYCLVPF-NNLGST 250
            +  +  GC    +     + +G +GL     SL+SQL      KFSYCLVPF +  G T
Sbjct: 205 SLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSINGKFSYCLVPFTSETGLT 264

Query: 251 SKMYFGSLPVTSGS---QTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDG-WI 310
           SK+ FG+  + SG     T ++  D   YY   L   +S    ++     ++   +G  +
Sbjct: 265 SKINFGTNGIVSGDGVVSTSMVKKDPATYYFLNLE-AISVGSKKIQFTSTIFGTGEGNIV 324

Query: 311 IDSGTTYSSLETDTF---DSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLESF--PD 370
           IDSGTT + L ++ +   +S++A  +      +  +DP     LC+     D  SF  PD
Sbjct: 325 IDSGTTLTLLPSNFYYELESVVASTIK----AERVQDPDGILSLCY----RDSSSFKVPD 384

Query: 371 VTVHFDGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVI 425
           +TVHF G D+ L   +TFV + +D + C AF  +   ++I GN    NF VGYD  +  +
Sbjct: 385 ITVHFKGGDVKLGNLNTFVAVSED-VSCFAF-AANEQLTIFGNLAQMNFLVGYDTVSGTV 429

BLAST of Bhi01G000682 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.6e-47
Identity = 138/432 (31.94%), Postives = 210/432 (48.61%), Query Frame = 0

Query: 11  GFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYL-YYNMLSENTLDNDVSLSP 70
           GF   L H DS  +      +T   +I+  ++R   RLN L    +L+  +  +D +   
Sbjct: 44  GFRLSLRHVDSGKN------LTKIQKIQRGINRGFHRLNRLGAVAVLAVASKPDDTNNIK 103

Query: 71  TLVNEG-GEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTTKFLSSK 130
              + G GE+L+  +IGNP  +     DT + LIW QC  C  +C    + PT  F   K
Sbjct: 104 APTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWTQCKPC-TEC---FDQPTPIFDPEK 163

Query: 131 SFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSDGKL 190
           S +Y    C S  CN+L     CN     C+Y   Y D  +T G+L++++F+F+  +   
Sbjct: 164 SSSYSKVGCSSGLCNALPR-SNCNEDKDACEYLYTYGDYSSTRGLLATETFTFEDEN--- 223

Query: 191 VDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNNLGSTSKM 250
             +  + FGC      +     +G VGL + PLSLISQL   KFSYCL    +  ++S +
Sbjct: 224 -SISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSIEDSEASSSL 283

Query: 251 YFGSLP-------------VTSGSQTPLLYPDSDA-YYVKVLGIGLSNDEPQLDR-VFDV 310
           + GSL                + + + L  PD  + YY+++ GI +      +++  F++
Sbjct: 284 FIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFEL 343

Query: 311 -YDVRDGWIIDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCF-AANANDL 370
             D   G IIDSGTT + LE   F  L  +F +   LP +        +LCF   +A   
Sbjct: 344 AEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDSGSTG-LDLCFKLPDAAKN 403

Query: 371 ESFPDVTVHFDGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDL 424
            + P +  HF GADL L  E+  V     G+ CLA + S + +SI GN Q QNF+V +DL
Sbjct: 404 IAVPKMIFHFKGADLELPGENYMVADSSTGVLCLA-MGSSNGMSIFGNVQQQNFNVLHDL 458

BLAST of Bhi01G000682 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.1e-61
Identity = 154/428 (35.98%), Postives = 230/428 (53.74%), Query Frame = 0

Query: 9   EVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTLDNDVSLS 68
           ++GFTA LIHRDSP SPFY+   T + R+   +HRS   +N +++    +NT    + L+
Sbjct: 28  KLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRS---VNRVFHFTEKDNTPQPQIDLT 87

Query: 69  PTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCS---DCNNQCEPKKEGPTTKFL 128
               +  GEYL++ +IG PP  +M  ADT + L+W QC+   DC  Q +P  +  T    
Sbjct: 88  ----SNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKT---- 147

Query: 129 SSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSD 188
              S TY+   C S+ C +L    +C+++D  C Y L Y DN  T G ++ D+ +  +SD
Sbjct: 148 ---SSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSD 207

Query: 189 GKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIK---KFSYCLVPF-NN 248
            + + +  +  GC    +    +  +G VGL   P+SLI QLG     KFSYCLVP  + 
Sbjct: 208 TRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTSK 267

Query: 249 LGSTSKMYFGSLPVTSGS---QTPLLYPDSDA--YYVKVLGIGLSNDEPQLDRVFDVYDV 308
              TSK+ FG+  + SGS    TPL+   S    YY+ +  I + + + Q     D    
Sbjct: 268 KDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSG-SDSESS 327

Query: 309 RDGWIIDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLESFPD 368
               IIDSGTT + L T+ +  L     +  D  ++K+DP+    LC++A   DL+  P 
Sbjct: 328 EGNIIIDSGTTLTLLPTEFYSELEDAVASSID-AEKKQDPQSGLSLCYSA-TGDLK-VPV 387

Query: 369 VTVHFDGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVI 425
           +T+HFDGAD+ L+  + FV++ +D + C AF R     SI GN    NF VGYD  ++ +
Sbjct: 388 ITMHFDGADVKLDSSNAFVQVSED-LVCFAF-RGSPSFSIYGNVAQMNFLVGYDTVSKTV 435

BLAST of Bhi01G000682 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 6.6e-54
Identity = 154/437 (35.24%), Postives = 223/437 (51.03%), Query Frame = 0

Query: 12  FTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTLDNDVSLSPTL 71
           F+  LIHRDSPLSP Y+  +T T R+ A   RS SR +  + + LS+  L +       L
Sbjct: 26  FSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSR-SRRFNHQLSQTDLQSG------L 85

Query: 72  VNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTTKFLSSKSFT 131
           +   GE+ +S  IG PP +V   ADT + L WVQC  C  QC  K+ GP   F   KS T
Sbjct: 86  IGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPC-QQCY-KENGPI--FDKKKSST 145

Query: 132 YEMEPCGSNFCNSLTGFQT-CNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSDGKLVD 191
           Y+ EPC S  C +L+  +  C+ S+  CKYR  Y D   + G +++++ S D++ G  V 
Sbjct: 146 YKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVS 205

Query: 192 VGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLG---IKKFSYCL---------VP 251
                FGC         ++ +G +GL    LSLISQLG    KKFSYCL           
Sbjct: 206 FPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLSHKSATTNGTS 265

Query: 252 FNNLGSTSKMYFGSLPVTSG-SQTPLLYPDSDAYY---VKVLGIGL-------SNDEPQL 311
             NLG+ S     SL   SG   TPL+  +   YY   ++ + +G        S+  P  
Sbjct: 266 VINLGTNS--IPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKIPYTGSSYNPND 325

Query: 312 DRVFDVYDVRDGWIIDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAAN 371
           D +    +     IIDSGTT + LE   FD   +         +   DP+     CF + 
Sbjct: 326 DGILS--ETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLSHCFKSG 385

Query: 372 ANDLESFPDVTVHFDGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHV 425
           + ++   P++TVHF GAD+ L+  + FVK+ +D + CL+ + + + V+I GNF   +F V
Sbjct: 386 SAEI-GLPEITVHFTGADVRLSPINAFVKLSED-MVCLSMVPT-TEVAIYGNFAQMDFLV 444

BLAST of Bhi01G000682 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.2e-42
Identity = 131/425 (30.82%), Postives = 194/425 (45.65%), Query Frame = 0

Query: 11  GFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTLDNDVSLSPT 70
           GF   L H DS  +      +T    +E  + R   RL  L      E  L+    +  +
Sbjct: 40  GFQIMLEHVDSGKN------LTKFQLLERAIERGSRRLQRL------EAMLNGPSGVETS 99

Query: 71  LVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTTKFLSSKSF 130
           +    GEYL++ +IG P        DT + LIW QC  C  QC  +    T  F    S 
Sbjct: 100 VYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPC-TQCFNQS---TPIFNPQGSS 159

Query: 131 TYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSDGKLVD 190
           ++   PC S  C +L+   TC  S+ +C+Y   Y D   T G + +++ +F +     V 
Sbjct: 160 SFSTLPCSSQLCQALSS-PTC--SNNFCQYTYGYGDGSETQGSMGTETLTFGS-----VS 219

Query: 191 VGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNNLGSTSKMYF 250
           +  + FGC E        +  G VG+ + PLSL SQL + KFSYC+ P  +  + S +  
Sbjct: 220 IPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGS-STPSNLLL 279

Query: 251 GSL--PVTSGSQTPLLYPDSDA---YYVKVLGIGLSNDEPQLDR---VFDVYDVRDGWII 310
           GSL   VT+GS    L   S     YY+ + G+ + +    +D      +  +   G II
Sbjct: 280 GSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIII 339

Query: 311 DSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLE-SFPDVTVHF 370
           DSGTT +    + + S+  +F++  +LP         F+LCF   ++      P   +HF
Sbjct: 340 DSGTTLTYFVNNAYQSVRQEFISQINLP-VVNGSSSGFDLCFQTPSDPSNLQIPTFVMHF 399

Query: 371 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 427
           DG DL L  E+ F+    +G+ CLA   S   +SI GN Q QN  V YD    V+SFA  
Sbjct: 400 DGGDLELPSENYFIS-PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASA 437

BLAST of Bhi01G000682 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 7.8e-39
Identity = 122/405 (30.12%), Postives = 187/405 (46.17%), Query Frame = 0

Query: 31  MTDTARIEATVHRSRSRLNYLYYNMLSENTLDNDVSLSPTLVNEGGEYLISFNIGNPPSQ 90
           +T    I+  + R   R+  +   + S + ++  V          GEYL++  IG P S 
Sbjct: 55  LTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAG------DGEYLMNVAIGTPDSS 114

Query: 91  VMGFADTSNGLIWVQCSDCNNQCEPKKEGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFQT 150
                DT + LIW QC  C  QC  +   PT  F    S ++   PC S +C  L   +T
Sbjct: 115 FSAIMDTGSDLIWTQCEPC-TQCFSQ---PTPIFNPQDSSSFSTLPCESQYCQDLPS-ET 174

Query: 151 CNSSDKWCKYRLVYADNKATSGILSSDSFSFDTSDGKLVDVGYLNFGCSEAPSTEDIQSY 210
           CN+++  C+Y   Y D   T G +++++F+F+TS      V  + FGC E        + 
Sbjct: 175 CNNNE--CQYTYGYGDGSTTQGYMATETFTFETS-----SVPNIAFGCGEDNQGFGQGNG 234

Query: 211 TGSVGLNQTPLSLISQLGIKKFSYCLVPFNNLGSTSKMYFGSLP--VTSGSQTPLLYPDS 270
            G +G+   PLSL SQLG+ +FSYC+  + +  S S +  GS    V  GS +  L   S
Sbjct: 235 AGLIGMGWGPLSLPSQLGVGQFSYCMTSYGS-SSPSTLALGSAASGVPEGSPSTTLIHSS 294

Query: 271 ---DAYYVKVLGIGLSNDEPQL-DRVFDVY-DVRDGWIIDSGTTYSSLETDTFDSLLAKF 330
                YY+ + GI +  D   +    F +  D   G IIDSGTT + L  D ++++   F
Sbjct: 295 LNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAF 354

Query: 331 LTLPDLPQEKKDPRDRFELCFAANAN-DLESFPDVTVHFDGADLILNVESTFVKIEDDGI 390
               +LP    +       CF   ++      P++++ FDG  L L  ++  +    +G+
Sbjct: 355 TDQINLP-TVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILIS-PAEGV 414

Query: 391 FCLAFLRSGS-PVSILGNFQLQNFHVGYDLEAQVISFAPVDCADS 427
            CLA   S    +SI GN Q Q   V YDL+   +SF P  C  S
Sbjct: 415 ICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQCGAS 438

BLAST of Bhi01G000682 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 6.4e-33
Identity = 115/366 (31.42%), Postives = 173/366 (47.27%), Query Frame = 0

Query: 76  GEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTTKFLSSKSFTYEME 135
           GEY     +G P   V    DT + ++W+QC+ C  +C  + +     F   KS TY   
Sbjct: 140 GEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC-RRCYSQSD---PIFDPRKSKTYATI 199

Query: 136 PCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSF--DTSDGKLVDVGY 195
           PC S  C  L     CN+  K C Y++ Y D   T G  S+++ +F  +   G  +  G+
Sbjct: 200 PCSSPHCRRLDS-AGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGH 259

Query: 196 LNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIK---KFSYCLVPFNNLGSTSKMYF 255
            N G              G +GL +  LS   Q G +   KFSYCLV  +     S + F
Sbjct: 260 DNEGL--------FVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVF 319

Query: 256 GSLPVTSGSQ-TPLL-YPDSDA-YYVKVLGI--------GLSNDEPQLDRVFDVYDVRDG 315
           G+  V+  ++ TPLL  P  D  YYV +LGI        G++    +LD++ +      G
Sbjct: 320 GNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGN-----GG 379

Query: 316 WIIDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCF-AANANDLESFPDVT 375
            IIDSGT+ + L    + ++   F       +   D    F+ CF  +N N+++  P V 
Sbjct: 380 VIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPD-FSLFDTCFDLSNMNEVK-VPTVV 439

Query: 376 VHFDGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISF 425
           +HF GAD+ L   +  + ++ +G FC AF  +   +SI+GN Q Q F V YDL +  + F
Sbjct: 440 LHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 485

BLAST of Bhi01G000682 vs. NCBI nr
Match: XP_008439375.1 (PREDICTED: aspartic proteinase CDR1-like [Cucumis melo])

HSP 1 Score: 776.2 bits (2003), Expect = 1.5e-220
Identity = 378/425 (88.94%), Postives = 393/425 (92.47%), Query Frame = 0

Query: 4   HTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYY-NMLSENTLD 63
           H V  EVGFTARLIH DSPLSPFY+HAMT TARIEATVHRSRSRL+YLYY N LSENTLD
Sbjct: 16  HMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLD 75

Query: 64  NDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTT 123
           NDVSLSPTLVNEGGEYL+SFNIGNPPSQVMGF DTSNGLIWVQCS+CN+QCEP+K GPTT
Sbjct: 76  NDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTT 135

Query: 124 KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFD 183
           KFLSSKSFTYEMEPCGSNFCNSLTGF+TCNSSDKWCKYRLVY DNKATSGILSSDSF FD
Sbjct: 136 KFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFD 195

Query: 184 TSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNNL 243
           TSDGKLVDVG+LNFGCSEAP T D QSYTG VGLNQTPLSLISQLGIKKFSYCLVPFN+L
Sbjct: 196 TSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNSL 255

Query: 244 GSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWII 303
           GSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI + NDEP  D VFDVYDVRDGWII
Sbjct: 256 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWII 315

Query: 304 DSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCF-AANANDLESFPDVTVHF 363
           D+G TYSSLETD FDSLLAKFL L + PQ K DP+DRFELCF  ANANDLESFPD TVHF
Sbjct: 316 DTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELANANDLESFPDATVHF 375

Query: 364 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 423
           DGADLILNVESTFVKIEDDGIFCLA LRSGSPVSILGNFQLQN+HVGYDLEAQVISFAPV
Sbjct: 376 DGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPV 435

Query: 424 DCADS 427
           DCADS
Sbjct: 436 DCADS 440

BLAST of Bhi01G000682 vs. NCBI nr
Match: KAA0049446.1 (aspartic proteinase CDR1-like [Cucumis melo var. makuwa] >TYK16125.1 aspartic proteinase CDR1-like [Cucumis melo var. makuwa])

HSP 1 Score: 776.2 bits (2003), Expect = 1.5e-220
Identity = 378/425 (88.94%), Postives = 393/425 (92.47%), Query Frame = 0

Query: 4   HTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYY-NMLSENTLD 63
           H V  EVGFTARLIH DSPLSPFY+HAMT TARIEATVHRSRSRL+YLYY N LSENTLD
Sbjct: 2   HMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLD 61

Query: 64  NDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTT 123
           NDVSLSPTLVNEGGEYL+SFNIGNPPSQVMGF DTSNGLIWVQCS+CN+QCEP+K GPTT
Sbjct: 62  NDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTT 121

Query: 124 KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFD 183
           KFLSSKSFTYEMEPCGSNFCNSLTGF+TCNSSDKWCKYRLVY DNKATSGILSSDSF FD
Sbjct: 122 KFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFD 181

Query: 184 TSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNNL 243
           TSDGKLVDVG+LNFGCSEAP T D QSYTG VGLNQTPLSLISQLGIKKFSYCLVPFN+L
Sbjct: 182 TSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNSL 241

Query: 244 GSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWII 303
           GSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI + NDEP  D VFDVYDVRDGWII
Sbjct: 242 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWII 301

Query: 304 DSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCF-AANANDLESFPDVTVHF 363
           D+G TYSSLETD FDSLLAKFL L + PQ K DP+DRFELCF  ANANDLESFPD TVHF
Sbjct: 302 DTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELANANDLESFPDATVHF 361

Query: 364 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 423
           DGADLILNVESTFVKIEDDGIFCLA LRSGSPVSILGNFQLQN+HVGYDLEAQVISFAPV
Sbjct: 362 DGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPV 421

Query: 424 DCADS 427
           DCADS
Sbjct: 422 DCADS 426

BLAST of Bhi01G000682 vs. NCBI nr
Match: XP_004134471.3 (aspartic proteinase CDR1 [Cucumis sativus] >KAE8650377.1 hypothetical protein Csa_011170 [Cucumis sativus])

HSP 1 Score: 772.3 bits (1993), Expect = 2.1e-219
Identity = 377/425 (88.71%), Postives = 391/425 (92.00%), Query Frame = 0

Query: 4   HTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYY-NMLSENTLD 63
           H V  EVGFTARLIH DSPLSPFY+H MTDTARIEATVHRSRSRLNYLYY N LSEN LD
Sbjct: 25  HMVSNEVGFTARLIHHDSPLSPFYNHTMTDTARIEATVHRSRSRLNYLYYINKLSENALD 84

Query: 64  NDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTT 123
           NDVSLSPTLVNEGGEYL+SFNIGNP SQVMGF DTSNGLIWVQCS+CN+QCEP+K G TT
Sbjct: 85  NDVSLSPTLVNEGGEYLMSFNIGNPSSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGLTT 144

Query: 124 KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFD 183
           KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVY DNKATSGILSSDSF FD
Sbjct: 145 KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFD 204

Query: 184 TSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNNL 243
           TSDG LVDVG+LNFGCSEAP T D QSYTG+VGLNQTPLSLISQLGIKKFSYCLVPFNNL
Sbjct: 205 TSDGMLVDVGFLNFGCSEAPLTGDEQSYTGNVGLNQTPLSLISQLGIKKFSYCLVPFNNL 264

Query: 244 GSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWII 303
           GSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI + NDEP  D VFDVY+VRDGWII
Sbjct: 265 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYEVRDGWII 324

Query: 304 DSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCF-AANANDLESFPDVTVHF 363
           D+G TYSSLETD FDSLLAKFLTL D PQ K DP++RFELCF   NANDLESFPDVTVHF
Sbjct: 325 DTGITYSSLETDAFDSLLAKFLTLKDFPQRKDDPKERFELCFELQNANDLESFPDVTVHF 384

Query: 364 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 423
           DGADLILNVESTFVKIEDDGIFCLA LRSGSPVSILGNFQLQN+HVGYDLEAQVISFAPV
Sbjct: 385 DGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPV 444

Query: 424 DCADS 427
           DCADS
Sbjct: 445 DCADS 449

BLAST of Bhi01G000682 vs. NCBI nr
Match: XP_023528351.1 (aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 768.1 bits (1982), Expect = 4.1e-218
Identity = 372/425 (87.53%), Postives = 397/425 (93.41%), Query Frame = 0

Query: 2   ARHTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTL 61
           ARH VPTEVGFTARLIHRDSP+SPFYDH MT+TA+IEATVHRSRSRLNYLYYNMLS+NTL
Sbjct: 23  ARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRLNYLYYNMLSKNTL 82

Query: 62  DNDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPT 121
           DND+SLSPTLV+EGGEYL+SFNIGNPPSQVMGFADTSNGLIWVQCSDCN+QCEP+K GP 
Sbjct: 83  DNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCNSQCEPEK-GPF 142

Query: 122 TKFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSF 181
           TKFL SKSFTYEMEPCGSN CNSLTGFQTCNSSD+WCKYRLVY DN  TSG LSSDSFSF
Sbjct: 143 TKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETSGNLSSDSFSF 202

Query: 182 DTSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNN 241
           DT+DGK VDVGYLNFGCSEAP T  +QSY GSVGLNQTPLSLISQLGIKKFSYCLVPF N
Sbjct: 203 DTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGIKKFSYCLVPF-N 262

Query: 242 LGSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWI 301
           LGSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI +  D+P LD VFDVYDVRDGWI
Sbjct: 263 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGVFDVYDVRDGWI 322

Query: 302 IDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLESFPDVTVHF 361
           IDSGTTYSSLETD FD LLAKF+TLPDL Q+K+DPR+RFELCFAANAND+E+FPDVTVH 
Sbjct: 323 IDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANANDMETFPDVTVHL 382

Query: 362 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 421
           DGA+LILNVESTFVKIEDDGI CLA LRSGSPVSILGNFQLQN+HVGYDLEAQV+SFAPV
Sbjct: 383 DGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPV 442

Query: 422 DCADS 427
           +CADS
Sbjct: 443 NCADS 445

BLAST of Bhi01G000682 vs. NCBI nr
Match: KAG6582237.1 (Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 760.8 bits (1963), Expect = 6.5e-216
Identity = 370/425 (87.06%), Postives = 391/425 (92.00%), Query Frame = 0

Query: 2   ARHTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTL 61
           ARH VPTEVGFTARLIHRDSPLSPFYDH MT+TARIEATVHRSRSRLNYLYYNMLS  TL
Sbjct: 14  ARHMVPTEVGFTARLIHRDSPLSPFYDHVMTNTARIEATVHRSRSRLNYLYYNMLSRKTL 73

Query: 62  DNDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPT 121
           DND+SLSPTLV+EGGEYL+SFNIGNP SQVMGFADTSNGLIWVQCSDCN+ CEP+K GP 
Sbjct: 74  DNDLSLSPTLVHEGGEYLMSFNIGNPSSQVMGFADTSNGLIWVQCSDCNSHCEPEK-GPF 133

Query: 122 TKFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSF 181
           TKFL SKSFTYEMEPCGSN CNSLTGFQTCNSSD+WCKYRLVY DN  TSG LSSDSFSF
Sbjct: 134 TKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETSGTLSSDSFSF 193

Query: 182 DTSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNN 241
           DT+DGK VDVGYLNFGCSEAP    +QSY GSVGLNQTPLSLISQLGIKKFSYCLVPF N
Sbjct: 194 DTTDGKHVDVGYLNFGCSEAPLIGGMQSYMGSVGLNQTPLSLISQLGIKKFSYCLVPF-N 253

Query: 242 LGSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWI 301
           LGSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI +  D+P LD VFDVYDVRDGWI
Sbjct: 254 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGTDDPNLDGVFDVYDVRDGWI 313

Query: 302 IDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLESFPDVTVHF 361
           IDSGTTYSSLETD FD LLAKF TLP+L Q+K+DPR+RFELCFAANAND+E+FPDVTVH 
Sbjct: 314 IDSGTTYSSLETDAFDRLLAKFNTLPELQQKKEDPRNRFELCFAANANDMETFPDVTVHL 373

Query: 362 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 421
           DGA+LILNVESTFVKIEDDGI CLA LRSGSPVSILGNFQLQN+HVGYDLEAQV+SFAPV
Sbjct: 374 DGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPV 433

Query: 422 DCADS 427
           DCADS
Sbjct: 434 DCADS 436

BLAST of Bhi01G000682 vs. ExPASy TrEMBL
Match: A0A5D3CXD4 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00490 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.2e-221
Identity = 378/425 (88.94%), Postives = 393/425 (92.47%), Query Frame = 0

Query: 4   HTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYY-NMLSENTLD 63
           H V  EVGFTARLIH DSPLSPFY+HAMT TARIEATVHRSRSRL+YLYY N LSENTLD
Sbjct: 2   HMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLD 61

Query: 64  NDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTT 123
           NDVSLSPTLVNEGGEYL+SFNIGNPPSQVMGF DTSNGLIWVQCS+CN+QCEP+K GPTT
Sbjct: 62  NDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTT 121

Query: 124 KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFD 183
           KFLSSKSFTYEMEPCGSNFCNSLTGF+TCNSSDKWCKYRLVY DNKATSGILSSDSF FD
Sbjct: 122 KFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFD 181

Query: 184 TSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNNL 243
           TSDGKLVDVG+LNFGCSEAP T D QSYTG VGLNQTPLSLISQLGIKKFSYCLVPFN+L
Sbjct: 182 TSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNSL 241

Query: 244 GSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWII 303
           GSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI + NDEP  D VFDVYDVRDGWII
Sbjct: 242 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWII 301

Query: 304 DSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCF-AANANDLESFPDVTVHF 363
           D+G TYSSLETD FDSLLAKFL L + PQ K DP+DRFELCF  ANANDLESFPD TVHF
Sbjct: 302 DTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELANANDLESFPDATVHF 361

Query: 364 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 423
           DGADLILNVESTFVKIEDDGIFCLA LRSGSPVSILGNFQLQN+HVGYDLEAQVISFAPV
Sbjct: 362 DGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPV 421

Query: 424 DCADS 427
           DCADS
Sbjct: 422 DCADS 426

BLAST of Bhi01G000682 vs. ExPASy TrEMBL
Match: A0A1S3AZA6 (aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103484191 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.2e-221
Identity = 378/425 (88.94%), Postives = 393/425 (92.47%), Query Frame = 0

Query: 4   HTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYY-NMLSENTLD 63
           H V  EVGFTARLIH DSPLSPFY+HAMT TARIEATVHRSRSRL+YLYY N LSENTLD
Sbjct: 16  HMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLD 75

Query: 64  NDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTT 123
           NDVSLSPTLVNEGGEYL+SFNIGNPPSQVMGF DTSNGLIWVQCS+CN+QCEP+K GPTT
Sbjct: 76  NDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTT 135

Query: 124 KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFD 183
           KFLSSKSFTYEMEPCGSNFCNSLTGF+TCNSSDKWCKYRLVY DNKATSGILSSDSF FD
Sbjct: 136 KFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFD 195

Query: 184 TSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNNL 243
           TSDGKLVDVG+LNFGCSEAP T D QSYTG VGLNQTPLSLISQLGIKKFSYCLVPFN+L
Sbjct: 196 TSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNSL 255

Query: 244 GSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWII 303
           GSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI + NDEP  D VFDVYDVRDGWII
Sbjct: 256 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWII 315

Query: 304 DSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCF-AANANDLESFPDVTVHF 363
           D+G TYSSLETD FDSLLAKFL L + PQ K DP+DRFELCF  ANANDLESFPD TVHF
Sbjct: 316 DTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELANANDLESFPDATVHF 375

Query: 364 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 423
           DGADLILNVESTFVKIEDDGIFCLA LRSGSPVSILGNFQLQN+HVGYDLEAQVISFAPV
Sbjct: 376 DGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPV 435

Query: 424 DCADS 427
           DCADS
Sbjct: 436 DCADS 440

BLAST of Bhi01G000682 vs. ExPASy TrEMBL
Match: A0A0A0L7U3 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G151520 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.0e-219
Identity = 377/425 (88.71%), Postives = 391/425 (92.00%), Query Frame = 0

Query: 4   HTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYY-NMLSENTLD 63
           H V  EVGFTARLIH DSPLSPFY+H MTDTARIEATVHRSRSRLNYLYY N LSEN LD
Sbjct: 2   HMVSNEVGFTARLIHHDSPLSPFYNHTMTDTARIEATVHRSRSRLNYLYYINKLSENALD 61

Query: 64  NDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPTT 123
           NDVSLSPTLVNEGGEYL+SFNIGNP SQVMGF DTSNGLIWVQCS+CN+QCEP+K G TT
Sbjct: 62  NDVSLSPTLVNEGGEYLMSFNIGNPSSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGLTT 121

Query: 124 KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSFD 183
           KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVY DNKATSGILSSDSF FD
Sbjct: 122 KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFD 181

Query: 184 TSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNNL 243
           TSDG LVDVG+LNFGCSEAP T D QSYTG+VGLNQTPLSLISQLGIKKFSYCLVPFNNL
Sbjct: 182 TSDGMLVDVGFLNFGCSEAPLTGDEQSYTGNVGLNQTPLSLISQLGIKKFSYCLVPFNNL 241

Query: 244 GSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWII 303
           GSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI + NDEP  D VFDVY+VRDGWII
Sbjct: 242 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYEVRDGWII 301

Query: 304 DSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCF-AANANDLESFPDVTVHF 363
           D+G TYSSLETD FDSLLAKFLTL D PQ K DP++RFELCF   NANDLESFPDVTVHF
Sbjct: 302 DTGITYSSLETDAFDSLLAKFLTLKDFPQRKDDPKERFELCFELQNANDLESFPDVTVHF 361

Query: 364 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 423
           DGADLILNVESTFVKIEDDGIFCLA LRSGSPVSILGNFQLQN+HVGYDLEAQVISFAPV
Sbjct: 362 DGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPV 421

Query: 424 DCADS 427
           DCADS
Sbjct: 422 DCADS 426

BLAST of Bhi01G000682 vs. ExPASy TrEMBL
Match: A0A6J1IXB1 (aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111479389 PE=3 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 1.7e-214
Identity = 369/425 (86.82%), Postives = 393/425 (92.47%), Query Frame = 0

Query: 2   ARHTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTL 61
           ARH VPTEVGFTARLIHRDSPLSPFYDH M+ TA IEAT+HRSRSRLNYLYYNMLS++TL
Sbjct: 23  ARHMVPTEVGFTARLIHRDSPLSPFYDHVMSYTAWIEATIHRSRSRLNYLYYNMLSKDTL 82

Query: 62  DNDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPT 121
           DND+SLSPTLV+EGGEYL+SFNIGNPPSQVMGFADTSNGLIWVQCSDCN+QCEP+K GP 
Sbjct: 83  DNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCNSQCEPEK-GPF 142

Query: 122 TKFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSF 181
           TKFL SKSFTYEMEPCGSN CNSLTGFQTCNSSD+ CKYRLVY DN  TSG LSSDSFSF
Sbjct: 143 TKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRSCKYRLVYEDNSETSGNLSSDSFSF 202

Query: 182 DTSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNN 241
           DT+DGK VDVGYLNFGCSEAP T  +QSY GSVGLNQTPLSLISQLGIKKFSYCLVPF N
Sbjct: 203 DTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGIKKFSYCLVPF-N 262

Query: 242 LGSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWI 301
           LGSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI +  D+P L+ VFDVYDVRDGWI
Sbjct: 263 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLEGVFDVYDVRDGWI 322

Query: 302 IDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLESFPDVTVHF 361
           IDSGTTYSSLETD FD LLAKF+TLPDL Q+K+DPR+RFELCFAANAND+E+FP VTVHF
Sbjct: 323 IDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANANDMETFPGVTVHF 382

Query: 362 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 421
           DGA+LILNVESTFVKIEDDGI CLA LRSGSPVSILGNFQLQN HVGYDLEAQV+SFAPV
Sbjct: 383 DGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNCHVGYDLEAQVVSFAPV 442

Query: 422 DCADS 427
           DCADS
Sbjct: 443 DCADS 445

BLAST of Bhi01G000682 vs. ExPASy TrEMBL
Match: A0A6J1GWK9 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111457819 PE=3 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 8.5e-214
Identity = 366/425 (86.12%), Postives = 390/425 (91.76%), Query Frame = 0

Query: 2   ARHTVPTEVGFTARLIHRDSPLSPFYDHAMTDTARIEATVHRSRSRLNYLYYNMLSENTL 61
           ARH VPT+VGFTARLIHRDSPLSPFY+H MT+TARIEATVHRSRSRLNYLYYNMLS  TL
Sbjct: 23  ARHMVPTDVGFTARLIHRDSPLSPFYNHVMTNTARIEATVHRSRSRLNYLYYNMLSRKTL 82

Query: 62  DNDVSLSPTLVNEGGEYLISFNIGNPPSQVMGFADTSNGLIWVQCSDCNNQCEPKKEGPT 121
           DND+SLSPTLV+EGGEYL+SFNIGNP SQVMGFADTSNGLIWVQCSDCN+ C+ +K GP 
Sbjct: 83  DNDLSLSPTLVHEGGEYLMSFNIGNPSSQVMGFADTSNGLIWVQCSDCNSHCDAEK-GPF 142

Query: 122 TKFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYADNKATSGILSSDSFSF 181
           TK L SKSFTYEMEPCGSN CNSLTGFQTCNSSD+WCKYRLVY DN  TSG LSSDSFSF
Sbjct: 143 TKLLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETSGTLSSDSFSF 202

Query: 182 DTSDGKLVDVGYLNFGCSEAPSTEDIQSYTGSVGLNQTPLSLISQLGIKKFSYCLVPFNN 241
           DT+DGK VDVGYLNFGCSEAP T  +QSY GSVGLNQTPLSLISQLGIKKFSYCLVPF N
Sbjct: 203 DTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGIKKFSYCLVPF-N 262

Query: 242 LGSTSKMYFGSLPVTSGSQTPLLYPDSDAYYVKVLGIGLSNDEPQLDRVFDVYDVRDGWI 301
           LGSTSKMYFGSLPVTSG QTPLLYP+SDAYYVKVLGI +  D+P LD VFDVYDVRDGWI
Sbjct: 263 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGVFDVYDVRDGWI 322

Query: 302 IDSGTTYSSLETDTFDSLLAKFLTLPDLPQEKKDPRDRFELCFAANANDLESFPDVTVHF 361
           IDSGTTYSSLETD FD LLAKF TLP+L Q+K+DPR+RFELCFAANAND+E+FPDVTVH 
Sbjct: 323 IDSGTTYSSLETDAFDRLLAKFNTLPELQQKKEDPRNRFELCFAANANDMETFPDVTVHL 382

Query: 362 DGADLILNVESTFVKIEDDGIFCLAFLRSGSPVSILGNFQLQNFHVGYDLEAQVISFAPV 421
           DGA+LILNVESTFVKIEDDGI CLA LRSGSPVSILGNFQLQN+HVGYDLEAQV+SFAPV
Sbjct: 383 DGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPV 442

Query: 422 DCADS 427
           DCADS
Sbjct: 443 DCADS 445

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G33340.18.0e-6335.98Eukaryotic aspartyl protease family protein [more]
AT2G35615.14.7e-5535.24Eukaryotic aspartyl protease family protein [more]
AT1G31450.11.3e-5233.64Eukaryotic aspartyl protease family protein [more]
AT1G64830.11.8e-5132.94Eukaryotic aspartyl protease family protein [more]
AT2G03200.11.6e-4731.94Eukaryotic aspartyl protease family protein [more]
Match NameE-valueIdentityDescription
Q6XBF81.1e-6135.98Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q3EBM56.6e-5435.24Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... [more]
Q766C31.2e-4230.82Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C27.8e-3930.12Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LNJ36.4e-3331.42Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_008439375.11.5e-22088.94PREDICTED: aspartic proteinase CDR1-like [Cucumis melo][more]
KAA0049446.11.5e-22088.94aspartic proteinase CDR1-like [Cucumis melo var. makuwa] >TYK16125.1 aspartic pr... [more]
XP_004134471.32.1e-21988.71aspartic proteinase CDR1 [Cucumis sativus] >KAE8650377.1 hypothetical protein Cs... [more]
XP_023528351.14.1e-21887.53aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo][more]
KAG6582237.16.5e-21687.06Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A5D3CXD47.2e-22188.94Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3AZA67.2e-22188.94aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103484191 PE=3 SV=1[more]
A0A0A0L7U31.0e-21988.71Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G15152... [more]
A0A6J1IXB11.7e-21486.82aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111479389 PE=3 S... [more]
A0A6J1GWK98.5e-21486.12aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111457819 PE=3... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 78..252
e-value: 1.0E-34
score: 120.3
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 255..426
e-value: 7.0E-38
score: 131.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 53..251
e-value: 3.6E-34
score: 120.4
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 71..423
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 270..419
e-value: 9.5E-25
score: 87.3
NoneNo IPR availablePANTHERPTHR47967:SF66ASPARTIC PROTEINASE CDR1-RELATEDcoord: 9..424
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 9..424
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 78..419
score: 27.783773
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 77..423
e-value: 1.01736E-64
score: 206.73

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000682Bhi01M000682mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
molecular_function GO:0016787 hydrolase activity