Bhi01G000637 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000637
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionadenylate isopentenyltransferase 5, chloroplastic
Locationchr1: 16337780 .. 16339834 (-)
RNA-Seq ExpressionBhi01G000637
SyntenyBhi01G000637
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCATAAATACCAAGTGTAGTCCTCTAATGGTTGAACCAAAGAGGGGCAGTGGCAATTGGAAGAAGAAGGGAAGATAATGTCACACATCATTTTCTTTCAAACCCACAAAATCCCAATTCCTTCTTAGAAATTAAAAACATTTCCATCATCCAAAATTTTTTCACATATTATTCCCCTCCAAAAAGGAAAAAAAAAAAAAAAATAGAAACTTTGGTGTTGCTTCAATTAGAGACATCATTAATGCCTAAACCCTCACTATCCATAAGCTAATCCTTTCAATTACTCTCTTGCATATACCCTACAAACCCTACTCCTCAACCCCTCTCTCTTTTTCTCTGTTTATCTTCAGGTACTGTTCTTTAATTGAGTAAGAACTCACACAAAAAGCATTTTTTTTTTTAAATAATCAAATGGGTGGATTAAAGTTTTCATTTTTTGAACTGGGTTTTGGTTAATTTTTACAGGGTATTTGATGAAGATGGCATTATCATTATCCTCTGCCGCCGGGAAGCAAGTCCAACCACTGGTGGGGAATTTTCAGGGGAATTTCAACATGGAACAGTTTTTCCGGCGAAAGGAGAAAGTTGTTGTTGTAATGGGGGCCACCGGAACGGGTAAATCCCGGTTAGCAATCGATTTAGCTACTCGATTTCCGGCAGAAATTGTCAACTCCGATAAGATTCAAGTTTACAAAGGGCTTGATGTTGTAACAAATAAAGTCACCGACGAAGAACGCCGTGGAATTTCACACCATTTGTTAGATTCAATTGATCCAAATTCAAATTTCTCTTCTCATGATTTTACTCGTCATGCCTCTGTTGCAATTGAATCAATTTTGGCTAGAGATCGCCTCCCAATTATCGCCGGTGGCTCTAATTCCTACATTGAAGCTCTGGTTGATGATTATGCAGAGTCCCGATTCCGATATGAATTCTGTTTTTTGTGGGTTGATGTTTCTTTACCGATTCTTCAGAAATTTGTCTCCGATCGTGTTGATCGGATGGTCGACGGTGGGTTTGTCGGAGAAGTGAGGCAAATTTTTGATCCGGAAGGGGATTATTCTTATGGGATTAAACGAGCCATTGGAGTGCCGGAATTACACGATTATTTGAGGGCAGAACACGACGGCGCCGATGAGAGGATTTTAACGATTCTTTTAGAATTGGCGATTTCGAAGATTAAAGATAATACTTGTCGATTGGCACTCCGGCAACTGGAGAAGATTCGCCGCCTTCGGAATAAATGGAATTGGAATCTCCGGCGACTTGATGCCACGGGAGCGTTTTTGACCGACGGTGGAAACTCCGATGACGCATGGCAAAAACTTGTCCTCGAACCAAGCTCGAGGATTGTCGACCAATTTCTCTGCGACGGTAACCGCCGTATCACCACCGCTATTGCCAGCCCGGAGCCGGTCCCCCGCGCCGTCGCCGCCGTAAGTCGTTAAAGTAATCCCAAACTTTTTCGACCCCACAGAATCTACGCTCCTCCCCAACAACGCGTTGACACCATTCCCGCGTGCCACGTGGCAAGCTTATAATTTTACCTTTTTTTTAAAAAAAAAAGAATTTTATCTTCTTTTTTTTTTTTGGTTTCGGTAATTTTAAATTAAAACAGGAAAAGGAAAAAAAAAGGAAGAAAATTACAACTTGGGACTGACAAAGTTTTCTGTCAGGTCGTTAAGTACTTACAATTGGCGGCCGTACAGATAAGCCGTCTATATTTAAAAAGAAAAAAGAAAAAGAAAAACATCTCTGCCGGCTCTGAAAAGTTCAAAAATAATTTGTTTTCTTTAAAAGAAAAAAGAAGAAAAAGAAATTGGAGAATCGTGTGTGGTGCAGCTACATCAGCTTCAATGCACCTCTCTGTTTGGCCCTCTATGATTGGGTGATTTTGAGAAGTAGGACCCACTCACCCTCACCCCCCGTGTGTATTAAAACCCTCTTCCTATGGAGTTTTTGGTCTTTTCGCTCCTTTTTGTGTATCTGTGGAAACTAATGAGCTGTATTTTTGTTAATATATCCATCTATCTGATTTTGAAATATTTTTGACTA

mRNA sequence

GGCATAAATACCAAGTGTAGTCCTCTAATGGTTGAACCAAAGAGGGGCAGTGGCAATTGGAAGAAGAAGGGAAGATAATGTCACACATCATTTTCTTTCAAACCCACAAAATCCCAATTCCTTCTTAGAAATTAAAAACATTTCCATCATCCAAAATTTTTTCACATATTATTCCCCTCCAAAAAGGAAAAAAAAAAAAAAAATAGAAACTTTGGTGTTGCTTCAATTAGAGACATCATTAATGCCTAAACCCTCACTATCCATAAGCTAATCCTTTCAATTACTCTCTTGCATATACCCTACAAACCCTACTCCTCAACCCCTCTCTCTTTTTCTCTGTTTATCTTCAGGGTATTTGATGAAGATGGCATTATCATTATCCTCTGCCGCCGGGAAGCAAGTCCAACCACTGGTGGGGAATTTTCAGGGGAATTTCAACATGGAACAGTTTTTCCGGCGAAAGGAGAAAGTTGTTGTTGTAATGGGGGCCACCGGAACGGGTAAATCCCGGTTAGCAATCGATTTAGCTACTCGATTTCCGGCAGAAATTGTCAACTCCGATAAGATTCAAGTTTACAAAGGGCTTGATGTTGTAACAAATAAAGTCACCGACGAAGAACGCCGTGGAATTTCACACCATTTGTTAGATTCAATTGATCCAAATTCAAATTTCTCTTCTCATGATTTTACTCGTCATGCCTCTGTTGCAATTGAATCAATTTTGGCTAGAGATCGCCTCCCAATTATCGCCGGTGGCTCTAATTCCTACATTGAAGCTCTGGTTGATGATTATGCAGAGTCCCGATTCCGATATGAATTCTGTTTTTTGTGGGTTGATGTTTCTTTACCGATTCTTCAGAAATTTGTCTCCGATCGTGTTGATCGGATGGTCGACGGTGGGTTTGTCGGAGAAGTGAGGCAAATTTTTGATCCGGAAGGGGATTATTCTTATGGGATTAAACGAGCCATTGGAGTGCCGGAATTACACGATTATTTGAGGGCAGAACACGACGGCGCCGATGAGAGGATTTTAACGATTCTTTTAGAATTGGCGATTTCGAAGATTAAAGATAATACTTGTCGATTGGCACTCCGGCAACTGGAGAAGATTCGCCGCCTTCGGAATAAATGGAATTGGAATCTCCGGCGACTTGATGCCACGGGAGCGTTTTTGACCGACGGTGGAAACTCCGATGACGCATGGCAAAAACTTGTCCTCGAACCAAGCTCGAGGATTGTCGACCAATTTCTCTGCGACGGTAACCGCCGTATCACCACCGCTATTGCCAGCCCGGAGCCGGTCCCCCGCGCCGTCGCCGCCGTAAGTCGTTAAAGTAATCCCAAACTTTTTCGACCCCACAGAATCTACGCTCCTCCCCAACAACGCGTTGACACCATTCCCGCGTGCCACGTGGCAAGCTTATAATTTTACCTTTTTTTTAAAAAAAAAAGAATTTTATCTTCTTTTTTTTTTTTGGTTTCGGTAATTTTAAATTAAAACAGGAAAAGGAAAAAAAAAGGAAGAAAATTACAACTTGGGACTGACAAAGTTTTCTGTCAGGTCGTTAAGTACTTACAATTGGCGGCCGTACAGATAAGCCGTCTATATTTAAAAAGAAAAAAGAAAAAGAAAAACATCTCTGCCGGCTCTGAAAAGTTCAAAAATAATTTGTTTTCTTTAAAAGAAAAAAGAAGAAAAAGAAATTGGAGAATCGTGTGTGGTGCAGCTACATCAGCTTCAATGCACCTCTCTGTTTGGCCCTCTATGATTGGGTGATTTTGAGAAGTAGGACCCACTCACCCTCACCCCCCGTGTGTATTAAAACCCTCTTCCTATGGAGTTTTTGGTCTTTTCGCTCCTTTTTGTGTATCTGTGGAAACTAATGAGCTGTATTTTTGTTAATATATCCATCTATCTGATTTTGAAATATTTTTGACTA

Coding sequence (CDS)

ATGAAGATGGCATTATCATTATCCTCTGCCGCCGGGAAGCAAGTCCAACCACTGGTGGGGAATTTTCAGGGGAATTTCAACATGGAACAGTTTTTCCGGCGAAAGGAGAAAGTTGTTGTTGTAATGGGGGCCACCGGAACGGGTAAATCCCGGTTAGCAATCGATTTAGCTACTCGATTTCCGGCAGAAATTGTCAACTCCGATAAGATTCAAGTTTACAAAGGGCTTGATGTTGTAACAAATAAAGTCACCGACGAAGAACGCCGTGGAATTTCACACCATTTGTTAGATTCAATTGATCCAAATTCAAATTTCTCTTCTCATGATTTTACTCGTCATGCCTCTGTTGCAATTGAATCAATTTTGGCTAGAGATCGCCTCCCAATTATCGCCGGTGGCTCTAATTCCTACATTGAAGCTCTGGTTGATGATTATGCAGAGTCCCGATTCCGATATGAATTCTGTTTTTTGTGGGTTGATGTTTCTTTACCGATTCTTCAGAAATTTGTCTCCGATCGTGTTGATCGGATGGTCGACGGTGGGTTTGTCGGAGAAGTGAGGCAAATTTTTGATCCGGAAGGGGATTATTCTTATGGGATTAAACGAGCCATTGGAGTGCCGGAATTACACGATTATTTGAGGGCAGAACACGACGGCGCCGATGAGAGGATTTTAACGATTCTTTTAGAATTGGCGATTTCGAAGATTAAAGATAATACTTGTCGATTGGCACTCCGGCAACTGGAGAAGATTCGCCGCCTTCGGAATAAATGGAATTGGAATCTCCGGCGACTTGATGCCACGGGAGCGTTTTTGACCGACGGTGGAAACTCCGATGACGCATGGCAAAAACTTGTCCTCGAACCAAGCTCGAGGATTGTCGACCAATTTCTCTGCGACGGTAACCGCCGTATCACCACCGCTATTGCCAGCCCGGAGCCGGTCCCCCGCGCCGTCGCCGCCGTAAGTCGTTAA

Protein sequence

MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIESILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNTCRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCDGNRRITTAIASPEPVPRAVAAVSR
Homology
BLAST of Bhi01G000637 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 360.5 bits (924), Expect = 1.4e-99
Identity = 192/325 (59.08%), Postives = 233/325 (71.69%), Query Frame = 0

Query: 9   SAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRFPAEIVNSD 68
           +A  + +QPL  NFQGN     FFRRK+KVV VMGATGTGKSRLAIDLATRFPAEIVNSD
Sbjct: 6   TALRQVIQPLSLNFQGNMVDVPFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSD 65

Query: 69  KIQVYKGLDVVTNKVTDEERRGISHHLLDSI-DPNSNFSSHDFTRHASVAIESILARDRL 128
           KIQVYKGLD+VTNKVT EE  G+ HHLL ++ D   +F++ DF R A  A+ESI+ RDR+
Sbjct: 66  KIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRV 125

Query: 129 PIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGFVGEVR 188
           PIIAGGSNSYIEALV+D  + R RY  CFLWVDVS P+L  FVS+RVD+MVD G V EVR
Sbjct: 126 PIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVR 185

Query: 189 QIFDP-EGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNTCRLALR 248
           +IFDP   DYS GI+RAIGVPEL ++LR+E           LLE AI KIK+NTC LA R
Sbjct: 186 RIFDPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACR 245

Query: 249 QLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFL-------C 308
           QL+KI+RL  +W WN+ R+DAT  FL  G  +D+AW   V  PS+  V++FL        
Sbjct: 246 QLQKIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFLSYSDDHHL 305

Query: 309 DGNRRITTAIASPEPVPRAVAAVSR 325
           +G   +   I++  P+P AVAA+SR
Sbjct: 306 EGANILLPEISAVPPLPAAVAAISR 330

BLAST of Bhi01G000637 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 300.1 bits (767), Expect = 2.2e-81
Identity = 163/306 (53.27%), Postives = 214/306 (69.93%), Query Frame = 0

Query: 3   MALSLSSAAGKQVQPLV--GNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 62
           M  S+SS   KQVQP++   N     N+  F   KEKV+ VMGATG+GKSRLAIDLATRF
Sbjct: 1   MKFSISSL--KQVQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATRF 60

Query: 63  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNS-NFSSHDFTRHASVAIE 122
             EI+NSDKIQ+YKGLDV+TNKVT +E RG+ HHLL   D  + N ++  ++R AS AI 
Sbjct: 61  QGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAIS 120

Query: 123 SILARDRLPIIAGGSNSYIEALVDDYAESRF-RYEFCFLWVDVSLPILQKFVSDRVDRMV 182
            + A ++LPI+AGGSNSYIEALV+  +      Y+ CF+WVDVSLP+L  FVS RVDRM+
Sbjct: 121 KLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMM 180

Query: 183 DGGFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKD 242
           + G + EVR++F+P+ +YS GI+RAIGVPELH+YLR E    D    + +L++A+  IK 
Sbjct: 181 EAGLLEEVREVFNPKANYSVGIRRAIGVPELHEYLRNE-SLVDRATKSKMLDVAVKNIKK 240

Query: 243 NTCRLALRQLEKIRRLRNKWNWNLRRLDATGAFL-TDGGNSDDAWQKLVLEPSSRIVDQF 302
           NT  LA RQL+KI+RL  KW  ++ R+DAT  FL  +    D+AW+ LV  PS RIVD+F
Sbjct: 241 NTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKF 300

Query: 303 LCDGNR 304
             + N+
Sbjct: 301 YNNNNQ 303

BLAST of Bhi01G000637 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 271.6 bits (693), Expect = 8.4e-73
Identity = 151/298 (50.67%), Postives = 199/298 (66.78%), Query Frame = 0

Query: 35  KEKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHH 94
           K+KVVV+MGATGTGKSRL++D+ATRF AEI+NSDKIQV++GLD+VTNK+T EE  G+ HH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 95  LLDSIDPNSNFSSHDFTRHASVAIESILARDRLPIIAGGSNSYIEALVDDYAES-RFRYE 154
           LL  + P ++ ++ ++   A+++IES+L R +LPII GGSNSY+EALVDD     R RY+
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYD 159

Query: 155 FCFLWVDVSLPILQKFVSDRVDRMVDGGFVGEVRQIFD-PEGDYSYGIKRAIGVPELHDY 214
            CFLWVDV+LP+L  FVS+RVD+MV+ G V EVR+ FD    DYS GIK+AIG PE   +
Sbjct: 160 CCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRF 219

Query: 215 LRAEHDGADERILTI-----LLELAISKIKDNTCRLALRQLEKIRRLRNKWNWNLRRLDA 274
            R      +E+ L +     LL   + +IK NT  LA RQ EKI RLR    W+++R+DA
Sbjct: 220 FR------NEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDA 279

Query: 275 TGAFLTDGGNSDD--AWQKLVLEPSSRIVDQFLCDGNRRITTAIASPEPVPRAVAAVS 324
           T  F       D   AW++LV  PS+  V +FL D        IAS  P+  A  AV+
Sbjct: 280 TPVFTKRRSKMDANVAWERLVAGPSTDTVSRFLLD--------IASRRPLVEASTAVA 323

BLAST of Bhi01G000637 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 217.6 bits (553), Expect = 1.4e-56
Identity = 127/312 (40.71%), Postives = 190/312 (60.90%), Query Frame = 0

Query: 16  QPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKG 75
           QPLV + +      +   RK+KVVV++GATG GKSRL++DLATRFP+EI+NSDKIQVY+G
Sbjct: 47  QPLVSSIR--MEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEG 106

Query: 76  LDVVTNKVTDEERRGISHHLLDSIDP-NSNFSSHDFTRHASVAIESILARDRLPIIAGGS 135
           L++ TN++T ++RRG+ HHLL  I+P +   ++ +F   AS  ++ I +R ++PIIAGGS
Sbjct: 107 LEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGS 166

Query: 136 NSYIEALVDDYAESRF--------------RYEFCFLWVDVSLPILQKFVSDRVDRMVDG 195
           NS++ AL+    + +F              RYE CF+WVDVS  +L +++  RVD M+D 
Sbjct: 167 NSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDS 226

Query: 196 GFVGEVRQIFDPEG---DYSYGIKRAIGVPELHDYLRAEHDGADERILTILL-----ELA 255
           G   E+ + +DP     +  +GI++AIGVPE   Y + E+    + I    L     + A
Sbjct: 227 GMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFK-EYPPEKKMIKWDALRKAAYDKA 286

Query: 256 ISKIKDNTCRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSD------DAWQKLV 299
           +  IK NT  LA RQ++KI  L++   W + R+DAT +F      S       + W++ V
Sbjct: 287 VDDIKRNTWTLAKRQVKKIEMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQV 346

BLAST of Bhi01G000637 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 212.6 bits (540), Expect = 4.7e-55
Identity = 128/295 (43.39%), Postives = 182/295 (61.69%), Query Frame = 0

Query: 28  MEQFFRRKEKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDVVTNKVTDEE 87
           MEQ +  K+KVVV+MGATG+GKS L+IDLATRF  EIVNSDKIQ Y GL V TN+++  E
Sbjct: 37  MEQSY--KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILE 96

Query: 88  RRGISHHLLDSIDP-NSNFSSHDFTRHASVAIESILARDRLPIIAGGSNSYIEALVDDYA 147
           R G+ HHLL  + P +S  ++ +F   AS +I  I AR  LPIIAGGSNS+I AL+ D  
Sbjct: 97  RCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRF 156

Query: 148 E-------------SRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGFVGEVRQIFDPE 207
           +             S  RYE CFLWVDVS+ +L +++S RVD+M++ G   E+   +DP 
Sbjct: 157 DPKTYPFSSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPR 216

Query: 208 GDYS----YGIKRAIGVPELHDYL------RAEHDGADERILTILLELAISKIKDNTCRL 267
              S    +GI + IG+PE   Y       R +     ++      + A+ +IK+NT RL
Sbjct: 217 YSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRL 276

Query: 268 ALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFL 299
           A +Q+E+I +L++   W+++RLDAT +F   G +S + W   VL+ S ++V +FL
Sbjct: 277 AKKQIERIMKLKSS-GWDIQRLDATPSF---GRSSREIWDNTVLDESIKVVKRFL 325

BLAST of Bhi01G000637 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 360.5 bits (924), Expect = 1.9e-98
Identity = 192/325 (59.08%), Postives = 233/325 (71.69%), Query Frame = 0

Query: 9   SAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRFPAEIVNSD 68
           +A  + +QPL  NFQGN     FFRRK+KVV VMGATGTGKSRLAIDLATRFPAEIVNSD
Sbjct: 6   TALRQVIQPLSLNFQGNMVDVPFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSD 65

Query: 69  KIQVYKGLDVVTNKVTDEERRGISHHLLDSI-DPNSNFSSHDFTRHASVAIESILARDRL 128
           KIQVYKGLD+VTNKVT EE  G+ HHLL ++ D   +F++ DF R A  A+ESI+ RDR+
Sbjct: 66  KIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRV 125

Query: 129 PIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGFVGEVR 188
           PIIAGGSNSYIEALV+D  + R RY  CFLWVDVS P+L  FVS+RVD+MVD G V EVR
Sbjct: 126 PIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVR 185

Query: 189 QIFDP-EGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNTCRLALR 248
           +IFDP   DYS GI+RAIGVPEL ++LR+E           LLE AI KIK+NTC LA R
Sbjct: 186 RIFDPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACR 245

Query: 249 QLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFL-------C 308
           QL+KI+RL  +W WN+ R+DAT  FL  G  +D+AW   V  PS+  V++FL        
Sbjct: 246 QLQKIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFLSYSDDHHL 305

Query: 309 DGNRRITTAIASPEPVPRAVAAVSR 325
           +G   +   I++  P+P AVAA+SR
Sbjct: 306 EGANILLPEISAVPPLPAAVAAISR 330

BLAST of Bhi01G000637 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 300.1 bits (767), Expect = 3.1e-80
Identity = 163/306 (53.27%), Postives = 214/306 (69.93%), Query Frame = 0

Query: 3   MALSLSSAAGKQVQPLV--GNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 62
           M  S+SS   KQVQP++   N     N+  F   KEKV+ VMGATG+GKSRLAIDLATRF
Sbjct: 1   MKFSISSL--KQVQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATRF 60

Query: 63  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNS-NFSSHDFTRHASVAIE 122
             EI+NSDKIQ+YKGLDV+TNKVT +E RG+ HHLL   D  + N ++  ++R AS AI 
Sbjct: 61  QGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAIS 120

Query: 123 SILARDRLPIIAGGSNSYIEALVDDYAESRF-RYEFCFLWVDVSLPILQKFVSDRVDRMV 182
            + A ++LPI+AGGSNSYIEALV+  +      Y+ CF+WVDVSLP+L  FVS RVDRM+
Sbjct: 121 KLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMM 180

Query: 183 DGGFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKD 242
           + G + EVR++F+P+ +YS GI+RAIGVPELH+YLR E    D    + +L++A+  IK 
Sbjct: 181 EAGLLEEVREVFNPKANYSVGIRRAIGVPELHEYLRNE-SLVDRATKSKMLDVAVKNIKK 240

Query: 243 NTCRLALRQLEKIRRLRNKWNWNLRRLDATGAFL-TDGGNSDDAWQKLVLEPSSRIVDQF 302
           NT  LA RQL+KI+RL  KW  ++ R+DAT  FL  +    D+AW+ LV  PS RIVD+F
Sbjct: 241 NTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKF 300

Query: 303 LCDGNR 304
             + N+
Sbjct: 301 YNNNNQ 303

BLAST of Bhi01G000637 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-71
Identity = 151/298 (50.67%), Postives = 199/298 (66.78%), Query Frame = 0

Query: 35  KEKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHH 94
           K+KVVV+MGATGTGKSRL++D+ATRF AEI+NSDKIQV++GLD+VTNK+T EE  G+ HH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 95  LLDSIDPNSNFSSHDFTRHASVAIESILARDRLPIIAGGSNSYIEALVDDYAES-RFRYE 154
           LL  + P ++ ++ ++   A+++IES+L R +LPII GGSNSY+EALVDD     R RY+
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYD 159

Query: 155 FCFLWVDVSLPILQKFVSDRVDRMVDGGFVGEVRQIFD-PEGDYSYGIKRAIGVPELHDY 214
            CFLWVDV+LP+L  FVS+RVD+MV+ G V EVR+ FD    DYS GIK+AIG PE   +
Sbjct: 160 CCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRF 219

Query: 215 LRAEHDGADERILTI-----LLELAISKIKDNTCRLALRQLEKIRRLRNKWNWNLRRLDA 274
            R      +E+ L +     LL   + +IK NT  LA RQ EKI RLR    W+++R+DA
Sbjct: 220 FR------NEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDA 279

Query: 275 TGAFLTDGGNSDD--AWQKLVLEPSSRIVDQFLCDGNRRITTAIASPEPVPRAVAAVS 324
           T  F       D   AW++LV  PS+  V +FL D        IAS  P+  A  AV+
Sbjct: 280 TPVFTKRRSKMDANVAWERLVAGPSTDTVSRFLLD--------IASRRPLVEASTAVA 323

BLAST of Bhi01G000637 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 6.8e-59
Identity = 130/310 (41.94%), Postives = 182/310 (58.71%), Query Frame = 0

Query: 25  NFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDVVTNKVT 84
           N ++ +   RKEK++V+MGATGTGKSRL+IDLA  FP E++NSDK+QVYKGLD+ TNK++
Sbjct: 19  NVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKIS 78

Query: 85  DEERRGISHHLLDSIDP-NSNFSSHDFTRHASVAIESILARDRLPIIAGGSNSYIEALVD 144
             +R G+ HHLL  +DP     +  DF   A  A+  I  R +LP++ GGSNS+I AL+ 
Sbjct: 79  VPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLV 138

Query: 145 D----------------YAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGFVGEVR 204
           D                   S  RY+ CFLWVDVS+ +L  +++ RVD M++ G   E+ 
Sbjct: 139 DRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELA 198

Query: 205 QIFDPEGD-------YSYGIKRAIGVPELHDYLR----AEHDGAD---ERILTILLELAI 264
           + + PE +          G+++AIGVPE   Y       + +G D   +R+     E A+
Sbjct: 199 EFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAV 258

Query: 265 SKIKDNTCRLALRQLEKIRRLRNKWNWNLRRLDATGAFLT-----DGGNSDDAWQKLVLE 299
             IK+NTC LA RQ+ KI RL+    W+LRRLDAT +F        G    + W+K VLE
Sbjct: 259 RAIKENTCHLAKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLE 318

BLAST of Bhi01G000637 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 217.6 bits (553), Expect = 2.0e-55
Identity = 127/312 (40.71%), Postives = 190/312 (60.90%), Query Frame = 0

Query: 16  QPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKG 75
           QPLV + +      +   RK+KVVV++GATG GKSRL++DLATRFP+EI+NSDKIQVY+G
Sbjct: 47  QPLVSSIR--MEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEG 106

Query: 76  LDVVTNKVTDEERRGISHHLLDSIDP-NSNFSSHDFTRHASVAIESILARDRLPIIAGGS 135
           L++ TN++T ++RRG+ HHLL  I+P +   ++ +F   AS  ++ I +R ++PIIAGGS
Sbjct: 107 LEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGS 166

Query: 136 NSYIEALVDDYAESRF--------------RYEFCFLWVDVSLPILQKFVSDRVDRMVDG 195
           NS++ AL+    + +F              RYE CF+WVDVS  +L +++  RVD M+D 
Sbjct: 167 NSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDS 226

Query: 196 GFVGEVRQIFDPEG---DYSYGIKRAIGVPELHDYLRAEHDGADERILTILL-----ELA 255
           G   E+ + +DP     +  +GI++AIGVPE   Y + E+    + I    L     + A
Sbjct: 227 GMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFK-EYPPEKKMIKWDALRKAAYDKA 286

Query: 256 ISKIKDNTCRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSD------DAWQKLV 299
           +  IK NT  LA RQ++KI  L++   W + R+DAT +F      S       + W++ V
Sbjct: 287 VDDIKRNTWTLAKRQVKKIEMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQV 346

BLAST of Bhi01G000637 vs. NCBI nr
Match: XP_038898938.1 (adenylate isopentenyltransferase 5, chloroplastic [Benincasa hispida])

HSP 1 Score: 644.0 bits (1660), Expect = 6.7e-181
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF
Sbjct: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES
Sbjct: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG
Sbjct: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT
Sbjct: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD
Sbjct: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
           GNRRITTAIASPEPVPRAVAAVSR
Sbjct: 301 GNRRITTAIASPEPVPRAVAAVSR 324

BLAST of Bhi01G000637 vs. NCBI nr
Match: KAA0049402.1 (adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK17157.1 adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 574.7 bits (1480), Expect = 5.0e-160
Identity = 288/324 (88.89%), Postives = 305/324 (94.14%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           M+MALSL+SAAGKQVQPLVGNFQGNFNMEQFFRRK+KVVVVMGATGTGKSRLAI+LATRF
Sbjct: 13  MRMALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRF 72

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           P+EIVNSDKIQVY+GLDVVTNKVT+EERRGI HHLL SIDP SNFSS DFT HASVAIES
Sbjct: 73  PSEIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASVAIES 132

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG
Sbjct: 133 ILARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 192

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFV EVRQIFDPEGDYS GIKRAIGVPELH+YLRAE DGADER+L ILLELAIS+IKDNT
Sbjct: 193 GFVEEVRQIFDPEGDYSQGIKRAIGVPELHEYLRAERDGADERVLKILLELAISRIKDNT 252

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           CRLA RQLEKIR L  KWNWNLRRLDATGAFLTDG NS D W+KLVLEPSSRIVDQFLCD
Sbjct: 253 CRLAFRQLEKIRLLHTKWNWNLRRLDATGAFLTDGENSVDVWEKLVLEPSSRIVDQFLCD 312

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
           G+RRITT I++P+ V RAVAAVSR
Sbjct: 313 GSRRITTGISTPDAVSRAVAAVSR 336

BLAST of Bhi01G000637 vs. NCBI nr
Match: XP_008438727.1 (PREDICTED: adenylate isopentenyltransferase 5, chloroplastic [Cucumis melo])

HSP 1 Score: 574.7 bits (1480), Expect = 5.0e-160
Identity = 288/324 (88.89%), Postives = 305/324 (94.14%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           M+MALSL+SAAGKQVQPLVGNFQGNFNMEQFFRRK+KVVVVMGATGTGKSRLAI+LATRF
Sbjct: 1   MRMALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRF 60

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           P+EIVNSDKIQVY+GLDVVTNKVT+EERRGI HHLL SIDP SNFSS DFT HASVAIES
Sbjct: 61  PSEIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASVAIES 120

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG
Sbjct: 121 ILARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFV EVRQIFDPEGDYS GIKRAIGVPELH+YLRAE DGADER+L ILLELAIS+IKDNT
Sbjct: 181 GFVEEVRQIFDPEGDYSQGIKRAIGVPELHEYLRAERDGADERVLKILLELAISRIKDNT 240

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           CRLA RQLEKIR L  KWNWNLRRLDATGAFLTDG NS D W+KLVLEPSSRIVDQFLCD
Sbjct: 241 CRLAFRQLEKIRLLHTKWNWNLRRLDATGAFLTDGENSVDVWEKLVLEPSSRIVDQFLCD 300

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
           G+RRITT I++P+ V RAVAAVSR
Sbjct: 301 GSRRITTGISTPDAVSRAVAAVSR 324

BLAST of Bhi01G000637 vs. NCBI nr
Match: XP_011650995.1 (adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus] >KGN57030.1 hypothetical protein Csa_010976 [Cucumis sativus])

HSP 1 Score: 568.9 bits (1465), Expect = 2.7e-158
Identity = 285/324 (87.96%), Postives = 304/324 (93.83%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           M+MALSL+SAAGKQVQPLVGNFQGNFNMEQFFRRK+KVVVVMGATGTGKSRLAI+LATRF
Sbjct: 1   MRMALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRF 60

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           P+EIVNSDKIQVY+GLDVVTNKVT+EERRGI HHLL SIDP SNFSS DFT HAS AIES
Sbjct: 61  PSEIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASGAIES 120

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG
Sbjct: 121 ILARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFV EVRQIFDPEGDYS GIKRAIGVPELH++LRAE DGADER+L ILLELAIS+IKDNT
Sbjct: 181 GFVEEVRQIFDPEGDYSQGIKRAIGVPELHEFLRAERDGADERVLNILLELAISRIKDNT 240

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           CRLA RQLEKIR LR+KWNWNLRRLDATG  LTDG NS D W+KLVLEPSSRIVDQFLCD
Sbjct: 241 CRLAFRQLEKIRLLRSKWNWNLRRLDATGVILTDGENSLDVWEKLVLEPSSRIVDQFLCD 300

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
           G+RRITT I++P+ V RAVAAVSR
Sbjct: 301 GSRRITTGISTPDAVSRAVAAVSR 324

BLAST of Bhi01G000637 vs. NCBI nr
Match: XP_023526587.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.5 bits (1443), Expect = 9.8e-156
Identity = 285/324 (87.96%), Postives = 299/324 (92.28%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           M MALSLSSA GKQVQPLVGNFQG+F+MEQFFRRKEKVV+VMGATGTGKSRLAIDLATRF
Sbjct: 1   MNMALSLSSAVGKQVQPLVGNFQGSFSMEQFFRRKEKVVIVMGATGTGKSRLAIDLATRF 60

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           PAEIVNSDKIQ Y+GLDVVTNKVTDEERRG+SHHLL SIDPNSNFSSHDFT HASVAIES
Sbjct: 61  PAEIVNSDKIQAYEGLDVVTNKVTDEERRGVSHHLLGSIDPNSNFSSHDFTYHASVAIES 120

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPI+AGGSNSYIEALVDD+AESRFRYEFCFLWVDVSLPILQKFVS+RVDRMVDG
Sbjct: 121 ILARDRLPIVAGGSNSYIEALVDDWAESRFRYEFCFLWVDVSLPILQKFVSNRVDRMVDG 180

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFV EVR+ FDPEGDYS GIKRAIGVPELHDYLRAE D ADER LTILLELAISKIKDNT
Sbjct: 181 GFVSEVREFFDPEGDYSRGIKRAIGVPELHDYLRAERDEADERTLTILLELAISKIKDNT 240

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           C+LALRQL+KIRRLRNK NWNLRRLDATGAFLTDGGN++DAW K VL+PS RIVDQFL D
Sbjct: 241 CQLALRQLQKIRRLRNKRNWNLRRLDATGAFLTDGGNANDAWDKFVLQPSLRIVDQFLSD 300

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
             R ITT I SPE  P  VAAVSR
Sbjct: 301 DGRLITTPIVSPEAAP-LVAAVSR 323

BLAST of Bhi01G000637 vs. ExPASy TrEMBL
Match: A0A5A7U0V6 (Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00010 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.4e-160
Identity = 288/324 (88.89%), Postives = 305/324 (94.14%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           M+MALSL+SAAGKQVQPLVGNFQGNFNMEQFFRRK+KVVVVMGATGTGKSRLAI+LATRF
Sbjct: 13  MRMALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRF 72

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           P+EIVNSDKIQVY+GLDVVTNKVT+EERRGI HHLL SIDP SNFSS DFT HASVAIES
Sbjct: 73  PSEIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASVAIES 132

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG
Sbjct: 133 ILARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 192

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFV EVRQIFDPEGDYS GIKRAIGVPELH+YLRAE DGADER+L ILLELAIS+IKDNT
Sbjct: 193 GFVEEVRQIFDPEGDYSQGIKRAIGVPELHEYLRAERDGADERVLKILLELAISRIKDNT 252

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           CRLA RQLEKIR L  KWNWNLRRLDATGAFLTDG NS D W+KLVLEPSSRIVDQFLCD
Sbjct: 253 CRLAFRQLEKIRLLHTKWNWNLRRLDATGAFLTDGENSVDVWEKLVLEPSSRIVDQFLCD 312

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
           G+RRITT I++P+ V RAVAAVSR
Sbjct: 313 GSRRITTGISTPDAVSRAVAAVSR 336

BLAST of Bhi01G000637 vs. ExPASy TrEMBL
Match: A0A1S3AXR7 (adenylate isopentenyltransferase 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483750 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.4e-160
Identity = 288/324 (88.89%), Postives = 305/324 (94.14%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           M+MALSL+SAAGKQVQPLVGNFQGNFNMEQFFRRK+KVVVVMGATGTGKSRLAI+LATRF
Sbjct: 1   MRMALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRF 60

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           P+EIVNSDKIQVY+GLDVVTNKVT+EERRGI HHLL SIDP SNFSS DFT HASVAIES
Sbjct: 61  PSEIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASVAIES 120

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG
Sbjct: 121 ILARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFV EVRQIFDPEGDYS GIKRAIGVPELH+YLRAE DGADER+L ILLELAIS+IKDNT
Sbjct: 181 GFVEEVRQIFDPEGDYSQGIKRAIGVPELHEYLRAERDGADERVLKILLELAISRIKDNT 240

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           CRLA RQLEKIR L  KWNWNLRRLDATGAFLTDG NS D W+KLVLEPSSRIVDQFLCD
Sbjct: 241 CRLAFRQLEKIRLLHTKWNWNLRRLDATGAFLTDGENSVDVWEKLVLEPSSRIVDQFLCD 300

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
           G+RRITT I++P+ V RAVAAVSR
Sbjct: 301 GSRRITTGISTPDAVSRAVAAVSR 324

BLAST of Bhi01G000637 vs. ExPASy TrEMBL
Match: A0A0A0L524 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G150100 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.3e-158
Identity = 285/324 (87.96%), Postives = 304/324 (93.83%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           M+MALSL+SAAGKQVQPLVGNFQGNFNMEQFFRRK+KVVVVMGATGTGKSRLAI+LATRF
Sbjct: 1   MRMALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRF 60

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           P+EIVNSDKIQVY+GLDVVTNKVT+EERRGI HHLL SIDP SNFSS DFT HAS AIES
Sbjct: 61  PSEIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASGAIES 120

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG
Sbjct: 121 ILARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFV EVRQIFDPEGDYS GIKRAIGVPELH++LRAE DGADER+L ILLELAIS+IKDNT
Sbjct: 181 GFVEEVRQIFDPEGDYSQGIKRAIGVPELHEFLRAERDGADERVLNILLELAISRIKDNT 240

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           CRLA RQLEKIR LR+KWNWNLRRLDATG  LTDG NS D W+KLVLEPSSRIVDQFLCD
Sbjct: 241 CRLAFRQLEKIRLLRSKWNWNLRRLDATGVILTDGENSLDVWEKLVLEPSSRIVDQFLCD 300

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
           G+RRITT I++P+ V RAVAAVSR
Sbjct: 301 GSRRITTGISTPDAVSRAVAAVSR 324

BLAST of Bhi01G000637 vs. ExPASy TrEMBL
Match: A0A6J1GV51 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457751 PE=4 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.7e-156
Identity = 284/324 (87.65%), Postives = 300/324 (92.59%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           M MALSLSSA GKQVQPLVGNFQG+F+MEQFFRRKEKVV+VMGATGTGKSRLAIDLATRF
Sbjct: 1   MNMALSLSSAVGKQVQPLVGNFQGSFSMEQFFRRKEKVVIVMGATGTGKSRLAIDLATRF 60

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           PAEIVNSDKIQ Y+GLDVVTNKVTDEERRG+SHHLL SIDPNSNFSSHDFT HASVAIES
Sbjct: 61  PAEIVNSDKIQAYEGLDVVTNKVTDEERRGVSHHLLGSIDPNSNFSSHDFTYHASVAIES 120

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPI+AGGSNSYIEALVDD+AESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG
Sbjct: 121 ILARDRLPIVAGGSNSYIEALVDDWAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFVGEVR+ FDPEGDYS GIKRAIGVPELHDYLRAE DGAD+R LTILLELAISKIKDNT
Sbjct: 181 GFVGEVREFFDPEGDYSRGIKRAIGVPELHDYLRAERDGADQRTLTILLELAISKIKDNT 240

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           C+LALRQL+KIRRLRNK NWNLRRLDATGAFLT+ GN +DAW K VL+PS RIVD+FL D
Sbjct: 241 CQLALRQLQKIRRLRNKRNWNLRRLDATGAFLTNSGNPNDAWDKFVLQPSLRIVDKFLTD 300

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
            +R ITT I SPE  P  VAAVSR
Sbjct: 301 DSRLITTPIVSPEAAP-LVAAVSR 323

BLAST of Bhi01G000637 vs. ExPASy TrEMBL
Match: A0A6J1ISD1 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111480109 PE=4 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.5e-154
Identity = 282/324 (87.04%), Postives = 296/324 (91.36%), Query Frame = 0

Query: 1   MKMALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRF 60
           M MALSLSSA GKQVQPLVGNFQG+F+MEQFFRRKEKVV+VMGATGTGKSRLAIDLATRF
Sbjct: 1   MNMALSLSSAVGKQVQPLVGNFQGSFSMEQFFRRKEKVVIVMGATGTGKSRLAIDLATRF 60

Query: 61  PAEIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIES 120
           PAEIVNSDKIQ Y+GLDVVTNKVTDEERRG+SHHLL SIDPNSNFSSHDFT HASVAIES
Sbjct: 61  PAEIVNSDKIQAYEGLDVVTNKVTDEERRGVSHHLLGSIDPNSNFSSHDFTYHASVAIES 120

Query: 121 ILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDG 180
           ILARDRLPI+AGGSNSYIEALVDD+AESRFRYEFCFLWVDVS+PILQKFVSDRVDRMVDG
Sbjct: 121 ILARDRLPIVAGGSNSYIEALVDDWAESRFRYEFCFLWVDVSMPILQKFVSDRVDRMVDG 180

Query: 181 GFVGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNT 240
           GFV EVR+ FDPEGDYS GIKRAIGVPELHDYLRAE D ADER LTILLELAISKIKDNT
Sbjct: 181 GFVSEVREFFDPEGDYSRGIKRAIGVPELHDYLRAESDDADERTLTILLELAISKIKDNT 240

Query: 241 CRLALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCD 300
           C+LALRQL+KIRRLRNK NWNL+RLDATG FLTDGGN +DAW   VL+PS RIVDQFL D
Sbjct: 241 CQLALRQLQKIRRLRNKRNWNLQRLDATGVFLTDGGNPNDAWDTFVLQPSLRIVDQFLSD 300

Query: 301 GNRRITTAIASPEPVPRAVAAVSR 325
             R ITT I SPE  P  VAAVSR
Sbjct: 301 DRRLITTPIVSPEAAP-LVAAVSR 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G19040.11.4e-9959.08isopentenyltransferase 5 [more]
AT3G23630.12.2e-8153.27isopentenyltransferase 7 [more]
AT3G63110.18.4e-7350.67isopentenyltransferase 3 [more]
AT1G68460.11.4e-5640.71isopentenyltransferase 1 [more]
AT3G19160.14.7e-5543.39ATP/ADP isopentenyltransferases [more]
Match NameE-valueIdentityDescription
Q94ID21.9e-9859.08Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID13.1e-8053.27Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q93WC91.2e-7150.67Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q5GHF76.8e-5941.94Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID32.0e-5540.71Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
XP_038898938.16.7e-181100.00adenylate isopentenyltransferase 5, chloroplastic [Benincasa hispida][more]
KAA0049402.15.0e-16088.89adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK17157.1 adenyl... [more]
XP_008438727.15.0e-16088.89PREDICTED: adenylate isopentenyltransferase 5, chloroplastic [Cucumis melo][more]
XP_011650995.12.7e-15887.96adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus] >KGN57030.1 ... [more]
XP_023526587.19.8e-15687.96adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
A0A5A7U0V62.4e-16088.89Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AXR72.4e-16088.89adenylate isopentenyltransferase 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A0A0L5241.3e-15887.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G150100 PE=3 SV=1[more]
A0A6J1GV514.7e-15687.65adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1ISD11.5e-15487.04adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01715IPPTcoord: 71..145
e-value: 1.1E-23
score: 84.3
coord: 149..253
e-value: 1.7E-11
score: 44.3
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 163..262
e-value: 7.1E-105
score: 352.0
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 23..303
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 35..298
e-value: 7.1E-105
score: 352.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 36..194
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 23..303
IPR018022IPP transferaseHAMAPMF_00185IPP_transcoord: 38..295
score: 16.472523

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000637Bhi01M000637mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
biological_process GO:0008033 tRNA processing
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity