Bhi01G000601 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000601
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionReceptor-like serine/threonine-protein kinase
Locationchr1: 15595946 .. 15598378 (-)
RNA-Seq ExpressionBhi01G000601
SyntenyBhi01G000601
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCCATGATATTTCTTTCATTTGTTCTTCTAAACTTCTATAAGTGTTTGGCTCAAAATGGCTCCCCCATAAGCCGGGGTTCCTCCATAGCTGCAGGATCTAACCATACTTGGCTTTCTCCATCTGGGGATTTTGCCTTCGGTTTCTATCGTCTTCCCAACAATCTCTACCTTGTCGGAATCTGGTTTGATAAAATCCCACAGACACTGGTTTGGTCGGCCAATCGTGACAATCCGGCACCACCAAATTCCCTCGTCAAGTTGAACAGAACCGGCCAGTTTGTGCTCTCGTTCCCAAATGGCATCATTATTCAACCAATATTCAAAGAGCAGCAGGCCCCAGCAAGTTCCGGCCAAATGCAGGACGATGGCAACTTCGTGTTGAAAGACTCCAATTTCGTGGCTGTCGGGCAAAGCTTTGATTCGCCGACAGACACGCTCCTCCCCGGACAGATTTTGGGGGTTGACAAAAAGATGTTCTCTGCAAAAAACATATCAGATTTCTCAACTGGGAATTTCATGTTGCAAATGCAAAAGGATGGGAATCTGGTGCTTTCCAATTACCACTTCTCCAATGTTGGGTATTGGTTCACAATAGCAACTAACATTGAAAACACTGTGTTAGTGTTTGATAAATCCTCTGCTTTAATGTACCTCACAAATAGAATTAACCCAAATGGACAGAGTTTACGCAATTTGACAGTCAATGTTCCAGCCCCAGTTGGAGATTACTACCACAGAGCAACAATAAATGTCCATGGCGATTTCAGGCAATATGTTTACCCGAAAAGTAACGGCAGTGAATGGATAAAGGTTTGGGGAGCCATGAGAGACCCTTGCCTTGTGAACACGGTTTGCGGCTTAAATGGGCTCTGCAAATCACCCGACAACGATACCGTAACCTGCGATTGCTTGCCAGGTTTTGTTCATTTGGATCCGGCTGATGCTTGGAAAGGCTGTCGACCGGAAACAGTAATCAATTACTGTATGGGAGATTCCGGGAAGAATTTTACCCTCCAGGTGATCGAGGATGTGGATATTGACTTGCCCCCTGAATCAGAGCCTTTCTCAGATTTGGTTCGTATGTTCAATGTCGATTTTGAGAGCTGCAAAAGAGCAATCATGGAAGATTGTTACGTTATGGCTGCAACTTGGAAGAATTCGACATGTCTGAAGAAGAGGACGCCGTTGATGAATGGTAGAAACACTAATATTACCAAAGGAACCAAAACTCTAATCAGAGTGCCTTTATTTTTTAAAAACAGTTCAGAAGTTCATGACGGCAAAAACGAAAACAATTTTAACTACCGAAAAGTCCTGGAGATTGGCAATATAATCGCCGGAGTTTTGGCTTTTTGCTTCGGTGCCGTTGTCGTTTTCTATCATCCAACGGCTCGAAGATTGATTAGGAGAAAGAACTTTTTAAGTGCGAGTGCCATTGGAATCAATTTCCGAGAATTCACTTTCCAGGAATTGGTTGATGCAACCGATGGATTCAAAAAGATCCTTGGCCAGGGAAGTTCTTGTAAAGTAGTTCGTGGGAATTTACACATAGACGGTATCGATGTGGAAATTGCAGTGAAAGTGCTCGACAAAATGACTGAAAGAACCGAGACAGAGTTCGTGACAGAGCTAAGAATCATCGGCCGAACATATCACACAAATTTGGTCAGATTGTTAGGTTACTGCATCGAAAACAAGAAGCAACTTCTTCTAGTGTACGAGCTAATGCCCAATGGAGCACTATCAAGATTCCTCTTCGACAATGGCGAAAACATGGGAGCACTATCAAGATTCCTCTTCGACAATGGCGAAAACACTATCAAGATTTCTAATTGTAATTGGACACAAAGGGTAGAAATCGCAAAGGGGATAGCTAGAGGGTTAGCGTACTTACACGAAGAATGCGAGACACAGATCATCCACTGCGATGTGAAGCCTCAAAACGTGCTGCTCGACGCCAATTACGCAGCGAAGATCGCAGATTTTGGGATATCGAAATTGTTGAAGAAGGACCAAACGAGAACGAACACAGAGGCGAGAGGAACGGCAGGGTACATGGCGCCAGAGTGGCTGAGGGGCGCTCCGGTGACGGCAAAGGTGGACGTGTACAGCTATGGGGTAATGCTATTGGAAATCATTTGTTGCAGAAGACACATTGAGTTGGATCGAGTGGAAGAAGAAAGTGAGGAGGAGGATTTGGTACTATCGAATTGGGTTCTGAGTCGCGCGGCGGCCGGGAATCTAGAGACGGTGGTCGGAGATAAGGCGGAAATTTTGATGGATTTCGAGAGGTTTAAACGAATGGCAATGGTGGGATTGTGGTGCATTCATCCAGATGCATCTCAAAGACCATCAATGAAGAAGGTTACCCAAATGTTGGAAGGCACCGCCGAAGTTGGAACCCCTCCTCTGCTTCTCTGTTCCTGA

mRNA sequence

ATGGCGTCCATGATATTTCTTTCATTTGTTCTTCTAAACTTCTATAAGTGTTTGGCTCAAAATGGCTCCCCCATAAGCCGGGGTTCCTCCATAGCTGCAGGATCTAACCATACTTGGCTTTCTCCATCTGGGGATTTTGCCTTCGGTTTCTATCGTCTTCCCAACAATCTCTACCTTGTCGGAATCTGGTTTGATAAAATCCCACAGACACTGGTTTGGTCGGCCAATCGTGACAATCCGGCACCACCAAATTCCCTCGTCAAGTTGAACAGAACCGGCCAGTTTGTGCTCTCGTTCCCAAATGGCATCATTATTCAACCAATATTCAAAGAGCAGCAGGCCCCAGCAAGTTCCGGCCAAATGCAGGACGATGGCAACTTCGTGTTGAAAGACTCCAATTTCGTGGCTGTCGGGCAAAGCTTTGATTCGCCGACAGACACGCTCCTCCCCGGACAGATTTTGGGGGTTGACAAAAAGATGTTCTCTGCAAAAAACATATCAGATTTCTCAACTGGGAATTTCATGTTGCAAATGCAAAAGGATGGGAATCTGGTGCTTTCCAATTACCACTTCTCCAATGTTGGGTATTGGTTCACAATAGCAACTAACATTGAAAACACTGTGTTAGTGTTTGATAAATCCTCTGCTTTAATGTACCTCACAAATAGAATTAACCCAAATGGACAGAGTTTACGCAATTTGACAGTCAATGTTCCAGCCCCAGTTGGAGATTACTACCACAGAGCAACAATAAATGTCCATGGCGATTTCAGGCAATATGTTTACCCGAAAAGTAACGGCAGTGAATGGATAAAGGTTTGGGGAGCCATGAGAGACCCTTGCCTTGTGAACACGGTTTGCGGCTTAAATGGGCTCTGCAAATCACCCGACAACGATACCGTAACCTGCGATTGCTTGCCAGGTTTTGTTCATTTGGATCCGGCTGATGCTTGGAAAGGCTGTCGACCGGAAACAGTAATCAATTACTGTATGGGAGATTCCGGGAAGAATTTTACCCTCCAGGTGATCGAGGATGTGGATATTGACTTGCCCCCTGAATCAGAGCCTTTCTCAGATTTGGTTCGTATGTTCAATGTCGATTTTGAGAGCTGCAAAAGAGCAATCATGGAAGATTGTTACGTTATGGCTGCAACTTGGAAGAATTCGACATGTCTGAAGAAGAGGACGCCGTTGATGAATGGTAGAAACACTAATATTACCAAAGGAACCAAAACTCTAATCAGAGTGCCTTTATTTTTTAAAAACAGTTCAGAAGTTCATGACGGCAAAAACGAAAACAATTTTAACTACCGAAAAGTCCTGGAGATTGGCAATATAATCGCCGGAGTTTTGGCTTTTTGCTTCGGTGCCGTTGTCGTTTTCTATCATCCAACGGCTCGAAGATTGATTAGGAGAAAGAACTTTTTAAGTGCGAGTGCCATTGGAATCAATTTCCGAGAATTCACTTTCCAGGAATTGGTTGATGCAACCGATGGATTCAAAAAGATCCTTGGCCAGGGAAGTTCTTGTAAAGTAGTTCGTGGGAATTTACACATAGACGGTATCGATGTGGAAATTGCAGTGAAAGTGCTCGACAAAATGACTGAAAGAACCGAGACAGAGTTCGTGACAGAGCTAAGAATCATCGGCCGAACATATCACACAAATTTGGTCAGATTGTTAGGTTACTGCATCGAAAACAAGAAGCAACTTCTTCTAGTGTACGAGCTAATGCCCAATGGAGCACTATCAAGATTCCTCTTCGACAATGGCGAAAACATGGGAGCACTATCAAGATTCCTCTTCGACAATGGCGAAAACACTATCAAGATTTCTAATTGTAATTGGACACAAAGGGTAGAAATCGCAAAGGGGATAGCTAGAGGGTTAGCGTACTTACACGAAGAATGCGAGACACAGATCATCCACTGCGATGTGAAGCCTCAAAACGTGCTGCTCGACGCCAATTACGCAGCGAAGATCGCAGATTTTGGGATATCGAAATTGTTGAAGAAGGACCAAACGAGAACGAACACAGAGGCGAGAGGAACGGCAGGGTACATGGCGCCAGAGTGGCTGAGGGGCGCTCCGGTGACGGCAAAGGTGGACGTGTACAGCTATGGGGTAATGCTATTGGAAATCATTTGTTGCAGAAGACACATTGAGTTGGATCGAGTGGAAGAAGAAAGTGAGGAGGAGGATTTGGTACTATCGAATTGGGTTCTGAGTCGCGCGGCGGCCGGGAATCTAGAGACGGTGGTCGGAGATAAGGCGGAAATTTTGATGGATTTCGAGAGGTTTAAACGAATGGCAATGGTGGGATTGTGGTGCATTCATCCAGATGCATCTCAAAGACCATCAATGAAGAAGGTTACCCAAATGTTGGAAGGCACCGCCGAAGTTGGAACCCCTCCTCTGCTTCTCTGTTCCTGA

Coding sequence (CDS)

ATGGCGTCCATGATATTTCTTTCATTTGTTCTTCTAAACTTCTATAAGTGTTTGGCTCAAAATGGCTCCCCCATAAGCCGGGGTTCCTCCATAGCTGCAGGATCTAACCATACTTGGCTTTCTCCATCTGGGGATTTTGCCTTCGGTTTCTATCGTCTTCCCAACAATCTCTACCTTGTCGGAATCTGGTTTGATAAAATCCCACAGACACTGGTTTGGTCGGCCAATCGTGACAATCCGGCACCACCAAATTCCCTCGTCAAGTTGAACAGAACCGGCCAGTTTGTGCTCTCGTTCCCAAATGGCATCATTATTCAACCAATATTCAAAGAGCAGCAGGCCCCAGCAAGTTCCGGCCAAATGCAGGACGATGGCAACTTCGTGTTGAAAGACTCCAATTTCGTGGCTGTCGGGCAAAGCTTTGATTCGCCGACAGACACGCTCCTCCCCGGACAGATTTTGGGGGTTGACAAAAAGATGTTCTCTGCAAAAAACATATCAGATTTCTCAACTGGGAATTTCATGTTGCAAATGCAAAAGGATGGGAATCTGGTGCTTTCCAATTACCACTTCTCCAATGTTGGGTATTGGTTCACAATAGCAACTAACATTGAAAACACTGTGTTAGTGTTTGATAAATCCTCTGCTTTAATGTACCTCACAAATAGAATTAACCCAAATGGACAGAGTTTACGCAATTTGACAGTCAATGTTCCAGCCCCAGTTGGAGATTACTACCACAGAGCAACAATAAATGTCCATGGCGATTTCAGGCAATATGTTTACCCGAAAAGTAACGGCAGTGAATGGATAAAGGTTTGGGGAGCCATGAGAGACCCTTGCCTTGTGAACACGGTTTGCGGCTTAAATGGGCTCTGCAAATCACCCGACAACGATACCGTAACCTGCGATTGCTTGCCAGGTTTTGTTCATTTGGATCCGGCTGATGCTTGGAAAGGCTGTCGACCGGAAACAGTAATCAATTACTGTATGGGAGATTCCGGGAAGAATTTTACCCTCCAGGTGATCGAGGATGTGGATATTGACTTGCCCCCTGAATCAGAGCCTTTCTCAGATTTGGTTCGTATGTTCAATGTCGATTTTGAGAGCTGCAAAAGAGCAATCATGGAAGATTGTTACGTTATGGCTGCAACTTGGAAGAATTCGACATGTCTGAAGAAGAGGACGCCGTTGATGAATGGTAGAAACACTAATATTACCAAAGGAACCAAAACTCTAATCAGAGTGCCTTTATTTTTTAAAAACAGTTCAGAAGTTCATGACGGCAAAAACGAAAACAATTTTAACTACCGAAAAGTCCTGGAGATTGGCAATATAATCGCCGGAGTTTTGGCTTTTTGCTTCGGTGCCGTTGTCGTTTTCTATCATCCAACGGCTCGAAGATTGATTAGGAGAAAGAACTTTTTAAGTGCGAGTGCCATTGGAATCAATTTCCGAGAATTCACTTTCCAGGAATTGGTTGATGCAACCGATGGATTCAAAAAGATCCTTGGCCAGGGAAGTTCTTGTAAAGTAGTTCGTGGGAATTTACACATAGACGGTATCGATGTGGAAATTGCAGTGAAAGTGCTCGACAAAATGACTGAAAGAACCGAGACAGAGTTCGTGACAGAGCTAAGAATCATCGGCCGAACATATCACACAAATTTGGTCAGATTGTTAGGTTACTGCATCGAAAACAAGAAGCAACTTCTTCTAGTGTACGAGCTAATGCCCAATGGAGCACTATCAAGATTCCTCTTCGACAATGGCGAAAACATGGGAGCACTATCAAGATTCCTCTTCGACAATGGCGAAAACACTATCAAGATTTCTAATTGTAATTGGACACAAAGGGTAGAAATCGCAAAGGGGATAGCTAGAGGGTTAGCGTACTTACACGAAGAATGCGAGACACAGATCATCCACTGCGATGTGAAGCCTCAAAACGTGCTGCTCGACGCCAATTACGCAGCGAAGATCGCAGATTTTGGGATATCGAAATTGTTGAAGAAGGACCAAACGAGAACGAACACAGAGGCGAGAGGAACGGCAGGGTACATGGCGCCAGAGTGGCTGAGGGGCGCTCCGGTGACGGCAAAGGTGGACGTGTACAGCTATGGGGTAATGCTATTGGAAATCATTTGTTGCAGAAGACACATTGAGTTGGATCGAGTGGAAGAAGAAAGTGAGGAGGAGGATTTGGTACTATCGAATTGGGTTCTGAGTCGCGCGGCGGCCGGGAATCTAGAGACGGTGGTCGGAGATAAGGCGGAAATTTTGATGGATTTCGAGAGGTTTAAACGAATGGCAATGGTGGGATTGTGGTGCATTCATCCAGATGCATCTCAAAGACCATCAATGAAGAAGGTTACCCAAATGTTGGAAGGCACCGCCGAAGTTGGAACCCCTCCTCTGCTTCTCTGTTCCTGA

Protein sequence

MASMIFLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLVGIWFDKIPQTLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQKDGNLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPAPVGDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVTCDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDLVRMFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNSSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASAIGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIELDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDASQRPSMKKVTQMLEGTAEVGTPPLLLCS
Homology
BLAST of Bhi01G000601 vs. TAIR 10
Match: AT5G60900.1 (receptor-like protein kinase 1 )

HSP 1 Score: 398.3 bits (1022), Expect = 1.5e-110
Identity = 277/821 (33.74%), Postives = 403/821 (49.09%), Query Frame = 0

Query: 20  QNGSPISRGSSIAAGSNH----TWLSPSGDFAFGFYRL-PNNLYLVGIWFDKI-PQTLVW 79
           +NGS +  G S+ A  +     +W SPSGDFAFGF ++ PN+ + + IWFDKI  +T+VW
Sbjct: 28  RNGS-VPVGESLTASESQQISSSWRSPSGDFAFGFRKIQPNDGFTLSIWFDKISDKTIVW 87

Query: 80  SANRDNP----APPNSLVKLNRTGQFVLSFPNGIIIQPIFKE-QQAPASSGQMQDDGNFV 139
            A   N      P  S V L   G  V++ P G   Q +++       S G+  DDGNFV
Sbjct: 88  HAQAVNTTTGLVPNGSKVTLTADGGLVIADPRG---QELWRALSGGSVSRGRFTDDGNFV 147

Query: 140 L-----KDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQKDGN 199
           L     +DS+ V +  SF++PTDTLLP Q + V + + S +  + F  G F L+++ DGN
Sbjct: 148 LFRDGSEDSDEV-LWSSFENPTDTLLPNQNIEVGRNLSSRRTETSFKKGRFSLRLEDDGN 207

Query: 200 L--------------VLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQ 259
           L              + S Y+ SN     T   N     LVF++S  +  L    +    
Sbjct: 208 LQLHSLNAETASESDIYSQYYESN-----TNDPNNPGIQLVFNQSGEIYVLQRNNSRFVV 267

Query: 260 SLRNLTVNVPAPVGDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGL 319
             R+   ++ AP                  Y+   S G           D  L N  CG 
Sbjct: 268 KDRDPDFSIAAPF-----------------YI---STGP----------DDALGNMACGY 327

Query: 320 NGLCKSPDNDTVTCDCLPGFVHLDPADAWKGCRPETVINYCMGDS-GKNFTLQVIEDVDI 379
           N +C   +N    C+C   FV  DP++ +  C P+  +  C  ++   N  + + E   I
Sbjct: 328 NNICSLGNNKRPKCECPERFVLKDPSNEYGDCLPDFEMQTCRPENQTANSDVNLYE--FI 387

Query: 380 DLPPESEPFSDLVRMFNVDFESCKRAIMEDCY---VMAATWKNSTCLKKRTPLMNGRNTN 439
            L   + PF D     N D E CK + + DC    V+  T ++  C KK+ PL +G  + 
Sbjct: 388 TLEKTNWPFGDYESYANYDEERCKASCLSDCLCAAVIFGTNRDLKCWKKKFPLSHGERSP 447

Query: 440 ITKGTKTLIRVPLFFKNSSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPT 499
               + T I+V    +N S                      IA V             P 
Sbjct: 448 -RGDSDTFIKV----RNRS----------------------IADV-------------PV 507

Query: 500 ARRLIRRKNFLSASAIGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHI-DGIDV 559
                ++ +++           FT+ EL +AT  F + LG+G+   V +G L +  G +V
Sbjct: 508 TGNRAKKLDWV-----------FTYGELAEATRDFTEELGRGAFGIVYKGYLEVAGGSEV 567

Query: 560 EIAVKVLDKMTERTETEFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALS 619
            +AVK LD++    E EF  E+++IG+ +H NLVRL+G+C E + Q ++VYE +P G L+
Sbjct: 568 TVAVKKLDRLDLDNEKEFKNEVKVIGQIHHKNLVRLIGFCNEGQSQ-MIVYEFLPQGTLA 627

Query: 620 RFLFDNGENMGALSRFLFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHC 679
            FLF                     +    +W  R  IA  IARG+ YLHEEC  QIIHC
Sbjct: 628 NFLF---------------------RRPRPSWEDRKNIAVAIARGILYLHEECSEQIIHC 687

Query: 680 DVKPQNVLLDANYAAKIADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDV 739
           D+KPQN+LLD  Y  +I+DFG++KLL  +QT T T  RGT GY+APEW R +P+T+KVDV
Sbjct: 688 DIKPQNILLDEYYTPRISDFGLAKLLLMNQTYTLTNIRGTKGYVAPEWFRNSPITSKVDV 725

Query: 740 YSYGVMLLEIICCRRHIELDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDF 799
           YSYGVMLLEI+CC++ ++L        E++++L NW       G LE +  D +E + D 
Sbjct: 748 YSYGVMLLEIVCCKKAVDL--------EDNVILINWAYDCFRQGRLEDLTEDDSEAMNDM 725

Query: 800 ERFKRMAMVGLWCIHPDASQRPSMKKVTQMLEGTAEVGTPP 806
           E  +R   + +WCI  +   RP+M+ VTQMLEG  +V  PP
Sbjct: 808 ETVERYVKIAIWCIQEEHGMRPNMRNVTQMLEGVIQVFDPP 725

BLAST of Bhi01G000601 vs. TAIR 10
Match: AT4G00340.1 (receptor-like protein kinase 4 )

HSP 1 Score: 329.3 bits (843), Expect = 8.5e-90
Identity = 259/817 (31.70%), Postives = 385/817 (47.12%), Query Frame = 0

Query: 24  PISRGSSIAAGSNHTWLSPSGDFAFGFYRLPN---NLYLVGIWFDKIP-QTLVWSANRDN 83
           P S  S +    N T LS    F  GF+   N   N YL GI +  +P  T VW ANR  
Sbjct: 18  PPSVQSKVIIKGNQTILSFKAIFRLGFFSTTNGSSNWYL-GISYASMPTPTHVWVANRIR 77

Query: 84  PA--PPNSLVKLNRTGQFVLS-FPNGIIIQPIFKEQQAPASSGQMQDDGNFVLKDSNFVA 143
           P   P +S ++L  TG  ++S   +G++ Q    + + P +  +  + GN +L + +   
Sbjct: 78  PVSDPDSSTLELTSTGYLIVSNLRDGVVWQ---TDNKQPGTDFRFSETGNLILINDDGSP 137

Query: 144 VGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQKDGNLVLSNYHFSNVGY 203
           V QSFD+PTDT LPG  +     M S +++ D S G + L++    N     Y      Y
Sbjct: 138 VWQSFDNPTDTWLPGMNVTGLTAMTSWRSLFDPSPGFYSLRLSPSFNEFQLVYK-GTTPY 197

Query: 204 WFTIATNIENTVLVFDKSSALMYLTNRINP--NGQSLRNLTVNVPAPVGDYYHRATINVH 263
           W T     E  V V + +   +Y  + +NP     S   +   + +       R  +  +
Sbjct: 198 WSTGNWTGEAFVGVPEMTIPYIYRFHFVNPYTPTASFWYIVPPLDSVSEPRLTRFMVGAN 257

Query: 264 GDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVTCDCLPGFVHLDP 323
           G  +QY +     S W   W    DPC V  +CG  G C S       C C+ GF   + 
Sbjct: 258 GQLKQYTWDPQTQS-WNMFWLQPEDPCRVYNLCGQLGFCSS--ELLKPCACIRGFRPRND 317

Query: 324 ADAWK------GCRPETVINYCMGDSG-KNFTLQVIEDVDIDLPPESEPFSDL-VRMFNV 383
           A AW+      GCR E       GDSG K+ T + + D+  D         D+ +    V
Sbjct: 318 A-AWRSDDYSDGCRREN------GDSGEKSDTFEAVGDLRYD--------GDVKMSRLQV 377

Query: 384 DFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNSS-- 443
              SC +  +           NS+C+       +   +N+    K L+  P   KNSS  
Sbjct: 378 SKSSCAKTCL----------GNSSCV----GFYHKEKSNL---CKILLESPNNLKNSSSW 437

Query: 444 -----------EVHDGKNENNFNYRKVLEIGNIIA--GVLAFCFGAVVVFYHPTARRLIR 503
                      E   G ++ N + + ++ + +++    VL F     ++    + +R   
Sbjct: 438 TGVSEDVLYIREPKKGNSKGNIS-KSIIILCSVVGSISVLGFTLLVPLILLKRSRKRKKT 497

Query: 504 RKNFLSASAIGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVL 563
           RK      A+ +N + F+F+EL  AT+GF   +G G    V +G L   G    +AVK L
Sbjct: 498 RKQDEDGFAV-LNLKVFSFKELQSATNGFSDKVGHGGFGAVFKGTL--PGSSTFVAVKRL 557

Query: 564 DKMTERTETEFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNG 623
           ++     E+EF  E+  IG   H NLVRL G+C EN  + LLVY+ MP G+LS +L    
Sbjct: 558 ER-PGSGESEFRAEVCTIGNIQHVNLVRLRGFCSENLHR-LLVYDYMPQGSLSSYLSRTS 617

Query: 624 ENMGALSRFLFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNV 683
             +                    +W  R  IA G A+G+AYLHE C   IIHCD+KP+N+
Sbjct: 618 PKL-------------------LSWETRFRIALGTAKGIAYLHEGCRDCIIHCDIKPENI 677

Query: 684 LLDANYAAKIADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVML 743
           LLD++Y AK++DFG++KLL +D +R     RGT GY+APEW+ G P+T K DVYS+G+ L
Sbjct: 678 LLDSDYNAKVSDFGLAKLLGRDFSRVLATMRGTWGYVAPEWISGLPITTKADVYSFGMTL 737

Query: 744 LEIICCRRHIELDR---VEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFK 803
           LE+I  RR++ ++     E+E+E E      W       GN+++VV  +     + E   
Sbjct: 738 LELIGGRRNVIVNSDTLGEKETEPEKWFFPPWAAREIIQGNVDSVVDSRLNGEYNTEEVT 769

Query: 804 RMAMVGLWCIHPDASQRPSMKKVTQMLEGTAEVGTPP 806
           RMA V +WCI  +   RP+M  V +MLEG  EV  PP
Sbjct: 798 RMATVAIWCIQDNEEIRPAMGTVVKMLEGVVEVTVPP 769

BLAST of Bhi01G000601 vs. TAIR 10
Match: AT2G19130.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 315.8 bits (808), Expect = 9.8e-86
Identity = 252/830 (30.36%), Postives = 380/830 (45.78%), Query Frame = 0

Query: 7   LSFVLLN--FYKCLAQNGSPI--SRGSSIAAGSNHTWLSPSGDFAFGFYR--LPNNLYLV 66
           +SF+ L   F+ C   +GS    +         + T +S  G +  GF++    +N Y +
Sbjct: 2   VSFLTLTSFFFICFFIHGSSAVDTISGDFTLSGDQTIVSSDGTYEMGFFKPGSSSNFY-I 61

Query: 67  GIWFDKIPQTLVWSANRDNP-APPNSLVKLNRTGQFVL---SFPNGIIIQPIFKEQQAPA 126
           G+W+ ++ QT++W ANRD   +  NS V     G  +L   ++   +    +       A
Sbjct: 62  GMWYKQLSQTILWVANRDKAVSDKNSSVFKISNGNLILLDGNYQTPVWSTGLNSTSSVSA 121

Query: 127 SSGQMQDDGNFVLKDS----NFVAVGQSFDSPTDTLLPGQILGVDK------KMFSAKNI 186
               +QDDGN VL+      +   + QSFD P DT LPG  + +DK      ++ S K++
Sbjct: 122 LEAVLQDDGNLVLRTGGSSLSANVLWQSFDHPGDTWLPGVKIRLDKRTGKSQRLTSWKSL 181

Query: 187 SDFSTGNFMLQMQKDGNLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINP 246
            D S G F L++ +     +  ++ SN  YW +   N ++ +        L Y+ N    
Sbjct: 182 EDPSPGLFSLELDESTAYKIL-WNGSN-EYWSSGPWNPQSRIFDSVPEMRLNYIYNFSFF 241

Query: 247 NGQSLRNLTVNVPAPVGDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTV 306
           +  +    T ++   +     R  ++V G  +Q+ + + N   W   W   R  C V   
Sbjct: 242 SNTTDSYFTYSIYNQLN--VSRFVMDVSGQIKQFTWLEGN-KAWNLFWSQPRQQCQVYRY 301

Query: 307 CGLNGLCKSPDNDTVTCDCLPGFVHL-----DPADAWKGCRPETVINYCMGDSGKNFTLQ 366
           CG  G+C   D     C C  GF  +     D  D   GC  +T +    GD  + F L 
Sbjct: 302 CGSFGICS--DKSEPFCRCPQGFRPMSQKDWDLKDYSAGCVRKTELQCSRGDINQFFRLP 361

Query: 367 VIEDVDIDLPPESEPFSDLVRMFNVDFESCKRAIMEDCYVMAATWK--NSTCLKKRTPLM 426
                ++ L   SE  +            C  A   DC   A  +   +S CL     ++
Sbjct: 362 -----NMKLADNSEVLT------RTSLSICASACQGDCSCKAYAYDEGSSKCLVWSKDVL 421

Query: 427 NGRNTNITKGTKTLIRVPLFFKNSSEVHDGKNENNFNYRKVLEIGNIIA--GVLAFCFGA 486
           N +          +  + L   +   V      NN    K L  G ++   GV+      
Sbjct: 422 NLQQLEDENSEGNIFYLRLAASDVPNVGASGKSNN----KGLIFGAVLGSLGVIVLVLLV 481

Query: 487 VVVFYHPTARRLIRRKNFLSASAIGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNL 546
           V++       R  RRK        G     F+++EL +AT  F   LG G    V +G L
Sbjct: 482 VILIL-----RYRRRKRMRGEKGDG-TLSAFSYRELQNATKNFSDKLGGGGFGSVFKGAL 541

Query: 547 HIDGIDVEIAVKVLDKMTERTETEFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYEL 606
                  +IAVK L+ +++  E +F TE+  IG   H NLVRL G+C E  K+ LLVY+ 
Sbjct: 542 PDSS---DIAVKRLEGISQ-GEKQFRTEVVTIGTIQHVNLVRLRGFCSEGSKK-LLVYDY 601

Query: 607 MPNGALSRFLFDNGENMGALSRFLFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEEC 666
           MPNG+L   LF N               E  I +    W  R +IA G ARGLAYLH+EC
Sbjct: 602 MPNGSLDSHLFLN-------------QVEEKIVL---GWKLRFQIALGTARGLAYLHDEC 661

Query: 667 ETQIIHCDVKPQNVLLDANYAAKIADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAP 726
              IIHCD+KP+N+LLD+ +  K+ADFG++KL+ +D +R  T  RGT GY+APEW+ G  
Sbjct: 662 RDCIIHCDIKPENILLDSQFCPKVADFGLAKLVGRDFSRVLTTMRGTRGYLAPEWISGVA 721

Query: 727 VTAKVDVYSYGVMLLEIICCRRHIELDRVEEESEEEDLVLSNWVLS-RAAAGNLETVVGD 786
           +TAK DVYSYG+ML E++  RR+      E+   E+     +W  +     G++ ++V  
Sbjct: 722 ITAKADVYSYGMMLFELVSGRRN-----TEQSENEKVRFFPSWAATILTKDGDIRSLVDP 776

Query: 787 KAE-ILMDFERFKRMAMVGLWCIHPDASQRPSMKKVTQMLEGTAEVGTPP 806
           + E   +D E   R   V  WCI  + S RP+M +V Q+LEG  EV  PP
Sbjct: 782 RLEGDAVDIEEVTRACKVACWCIQDEESHRPAMSQVVQILEGVLEVNPPP 776

BLAST of Bhi01G000601 vs. TAIR 10
Match: AT1G34300.1 (lectin protein kinase family protein )

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-79
Identity = 238/807 (29.49%), Postives = 376/807 (46.59%), Query Frame = 0

Query: 23  SPISRGSSI-AAGSNHTWLSPSGDFAFGFYRLPN-NLYLVGIWF-DKIPQTLVWSANRDN 82
           S I  GS I A+GSN  W SP+  F+  F   P+ N +L  + F   +P   +WSA    
Sbjct: 25  STIPLGSVIYASGSNQNWPSPNSTFSVSFVPSPSPNSFLAAVSFAGSVP---IWSA---G 84

Query: 83  PAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQDDGNFVLKDSNFVAVGQ 142
                  ++L+ +G   L+  +G  +    K  +   +SG ++D G F+L ++  V V  
Sbjct: 85  TVDSRGSLRLHTSGSLRLTNGSGTTVWD-SKTDRLGVTSGSIEDTGEFILLNNRSVPVWS 144

Query: 143 SFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQKDGNLVL---SNYHFSNVGY 202
           SFD+PTDT+       V  + F+A  I    +G +  Q+++ GNL L   ++  + N G 
Sbjct: 145 SFDNPTDTI-------VQSQNFTAGKI--LRSGLYSFQLERSGNLTLRWNTSAIYWNHGL 204

Query: 203 WFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPAPVGDY-----YHRATI 262
             + ++N+ +  L    +  +    + +    + + +         GDY     +    +
Sbjct: 205 NSSFSSNLSSPRLSLQTNGVVSIFESNLLGGAEIVYS---------GDYGDSNTFRFLKL 264

Query: 263 NVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVTCDC-LPGFV 322
           +  G+ R Y     N       W A+ D CLV   CG  G+C   D + + C C    F 
Sbjct: 265 DDDGNLRIYSSASRNSGPVNAHWSAV-DQCLVYGYCGNFGICSYNDTNPI-CSCPSRNFD 324

Query: 323 HLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDLVRMFNVDFES 382
            +D  D  KGC+ +  ++ C G    N T+  +    +    E +P S+    F      
Sbjct: 325 FVDVNDRRKGCKRKVELSDCSG----NTTMLDLVHTRL-FTYEDDPNSE---SFFAGSSP 384

Query: 383 CKRAIMEDCYVMAATWK---NSTCLKKRT-PLMNGRNTNITKGTKTLIRVPLFFKNSSEV 442
           C+   +     +A+      +  C +K       G        T  +        N+ E 
Sbjct: 385 CRANCLSSVLCLASVSMSDGSGNCWQKHPGSFFTGYQWPSVPSTSYVKVCGPVVANTLER 444

Query: 443 HDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFY-----HPTARRLIRRKNFLS-ASA 502
               ++NN      +    +IAG+L      + +++     +P    L      L  AS 
Sbjct: 445 ATKGDDNNSKVHLWIVAVAVIAGLLGLVAVEIGLWWCCCRKNPRFGTLSSHYTLLEYASG 504

Query: 503 IGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTET 562
             +   +FT++EL   T  FK+ LG G    V RG L    +   +AVK L+ + E+ E 
Sbjct: 505 APV---QFTYKELQRCTKSFKEKLGAGGFGTVYRGVLTNRTV---VAVKQLEGI-EQGEK 564

Query: 563 EFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRF 622
           +F  E+  I  T+H NLVRL+G+C + + + LLVYE M NG+L  FLF         ++F
Sbjct: 565 QFRMEVATISSTHHLNLVRLIGFCSQGRHR-LLVYEFMRNGSLDNFLFTTDS-----AKF 624

Query: 623 LFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAK 682
           L              W  R  IA G A+G+ YLHEEC   I+HCD+KP+N+L+D N+AAK
Sbjct: 625 L-------------TWEYRFNIALGTAKGITYLHEECRDCIVHCDIKPENILVDDNFAAK 684

Query: 683 IADFGISKLLKKDQTRTN-TEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRR 742
           ++DFG++KLL     R N +  RGT GY+APEWL   P+T+K DVYSYG++LLE++  +R
Sbjct: 685 VSDFGLAKLLNPKDNRYNMSSVRGTRGYLAPEWLANLPITSKSDVYSYGMVLLELVSGKR 744

Query: 743 HIELDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDK--AEILMDFERFKRMAMVGLWC 802
           + +   V E++  +    S W       GN + ++  +   +  +D E+  RM     WC
Sbjct: 745 NFD---VSEKTNHKK--FSIWAYEEFEKGNTKAILDTRLSEDQTVDMEQVMRMVKTSFWC 765

Query: 803 IHPDASQRPSMKKVTQMLEGTAEVGTP 805
           I     QRP+M KV QMLEG  E+  P
Sbjct: 805 IQEQPLQRPTMGKVVQMLEGITEIKNP 765

BLAST of Bhi01G000601 vs. TAIR 10
Match: AT5G35370.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 265.8 bits (678), Expect = 1.2e-70
Identity = 226/824 (27.43%), Postives = 368/824 (44.66%), Query Frame = 0

Query: 40  LSPSGDFAFGFYRLPNNLYLVGIWFDKI---PQTLVWSANRDNPAPPNSLVKLNRTGQFV 99
           LS +  F  G +    +    G +F  +     + +WS+NRD+P   +S   +N T Q +
Sbjct: 51  LSRNSIFKAGLFSPGGDDSSTGFYFSVVHVDSGSTIWSSNRDSPV--SSSGTMNLTPQGI 110

Query: 100 LSFPNGIIIQPIFKEQ--QAPASSGQMQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQIL 159
               +G    P++      +P  S ++ D GN +L D   V++ +SFD PTD+++ GQ L
Sbjct: 111 SVIEDGKSQIPVWSTPVLASPVKSLRLTDAGNLLLLDHLNVSLWESFDFPTDSIVLGQRL 170

Query: 160 GVDKKMFSAKNISDFSTGNFMLQMQKDGNLVLSNYHFSNVGYWFTIATNIENTVLVFDKS 219
            +   +  + + SDFSTG++   + +   L+     +    YW  +  +I   V   D +
Sbjct: 171 KLGMFLSGSVSRSDFSTGDYKFLVGESDGLM----QWRGQNYW-KLRMHIRANV---DSN 230

Query: 220 SALMYLTNRINPNGQSLRN---LTVNVPAPVGDYYHRATINVHGDFRQYVYPKSNGSEWI 279
             + YLT   +      RN   + V V  P    +  A ++  G F   +  + +G   +
Sbjct: 231 FPVEYLTVTTSGLALMARNGTVVVVRVALPPSSDFRVAKMDSSGKF---IVSRFSGKNLV 290

Query: 280 KVWGAMRDPCLVNTVCGLNGLCKSPD-NDTVTCDCLPGFVHLDPADAWKG---------- 339
             +    D C +  VCG  GLC   + ++  +C C P  + +   DA KG          
Sbjct: 291 TEFSGPMDSCQIPFVCGKLGLCNLDNASENQSCSC-PDEMRM---DAGKGVCVPVSQSLS 350

Query: 340 ----CRPETVINYCMGDSGKNFTLQVIEDVDIDLPPES--EPFSDLVRMFNVDFESCKRA 399
               C    +    +G     F+    + V+  LP  +  +  S       V +E+  R+
Sbjct: 351 LPVSCEARNISYLELGLGVSYFSTHFTDPVEHGLPLLACHDICSKNCSCLGVFYENTSRS 410

Query: 400 IMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNSSEVHDGKNENN 459
               CY++  ++  S  L K +P     N ++    K  IR     K +++     N   
Sbjct: 411 ----CYLVKDSF-GSLSLVKNSP----ENHDLIGYVKLSIR-----KTNAQPPGNNNRGG 470

Query: 460 FNYRKVLEIGNIIAGVLAFCFG--------------AVVVFYHPTARRLIRRKNFLSASA 519
            ++        +IA VL  C G                V+ Y     + + R     +  
Sbjct: 471 SSF-------PVIALVLLPCSGFFLLIALGLLWWRRCAVMRYSSIREKQVTRPGSFESGD 530

Query: 520 IGINF-----REFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMT 579
           +G        ++F F+EL  AT+ FK  +G G    V +G L  + +   IAVK +    
Sbjct: 531 LGSFHIPGLPQKFEFEELEQATENFKMQIGSGGFGSVYKGTLPDETL---IAVKKITNHG 590

Query: 580 ERTETEFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMG 639
                EF TE+ IIG   HTNLV+L G+C    +QLLLVYE M +G+L + LF       
Sbjct: 591 LHGRQEFCTEIAIIGNIRHTNLVKLRGFCARG-RQLLLVYEYMNHGSLEKTLF------- 650

Query: 640 ALSRFLFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDA 699
                   +G   +      W +R +IA G ARGLAYLH  C+ +IIHCDVKP+N+LL  
Sbjct: 651 --------SGNGPV----LEWQERFDIALGTARGLAYLHSGCDQKIIHCDVKPENILLHD 710

Query: 700 NYAAKIADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEII 759
           ++  KI+DFG+SKLL ++++   T  RGT GY+APEW+  A ++ K DVYSYG++LLE++
Sbjct: 711 HFQPKISDFGLSKLLNQEESSLFTTMRGTRGYLAPEWITNAAISEKADVYSYGMVLLELV 770

Query: 760 CCRRHIELDRVEEESEEED---------------LVLSNWVLSRAAAGNLETVVGDKAEI 805
             R++    R    S  ED               +    + L     G    +   + E 
Sbjct: 771 SGRKNCSF-RSRSNSVTEDNNQNHSSTTTTSTGLVYFPLYALDMHEQGRYMELADPRLEG 812

BLAST of Bhi01G000601 vs. ExPASy Swiss-Prot
Match: Q7FAZ3 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 OS=Oryza sativa subsp. japonica OX=39947 GN=LECRK1 PE=2 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 3.2e-134
Identity = 311/851 (36.55%), Postives = 448/851 (52.64%), Query Frame = 0

Query: 1   MASMIFLSFVLLNFYKCLAQNGSPISRGSSIAAGS-NHTWLSPSGDFAFGFYRLPNN--L 60
           M +++    +L       AQ    I+ GS++A  S   +WLSPSGDFAFGF  +  N   
Sbjct: 1   MVALLLFPMLLQLLSPTCAQTQKNITLGSTLAPQSPASSWLSPSGDFAFGFRPVEGNTSF 60

Query: 61  YLVGIWFDKI-PQTLVWSA-NRDN-----PAPPNSLVKLNRTGQFVLSFPNGIIIQPIFK 120
           YL+ +WF+KI  +T+VW A N D        P +S ++L   G   L   +G   Q  + 
Sbjct: 61  YLIAVWFNKISDKTVVWYAKNTDQDPSIVEVPSDSFLQLTNDGALSLKDRSG---QEGWN 120

Query: 121 EQQAPASSGQMQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDK---KMFSAK-NI 180
            Q    +   M+D GNFVL  ++     Q+FD P+DT+LP Q++  +K   K   A+ +I
Sbjct: 121 PQVTSVAYASMRDTGNFVLLGADGTTKWQTFDMPSDTILPTQVIPCNKTRNKSLRARLDI 180

Query: 181 SDFSTGNFMLQMQKDGNLVL--------SNYHFSNVGYWFTIATNIENTVLVFDKSSALM 240
           +D+S+G F+L +Q DGNL L        S Y      YW T  T    + LVF ++  + 
Sbjct: 181 NDYSSGRFLLDVQTDGNLALYLVAVPSGSKYQ----QYWSTDTTG-NGSELVFSETGKVY 240

Query: 241 Y-LTNRINPNGQSLRNLTVNVPAPVGDYYHRATINVHGDFRQYVYPKS------NGSEWI 300
           + LT+    N  S   +       + DY+HRAT++  G FRQYVYPK        G  W 
Sbjct: 241 FALTDGTQINISSGAGI-----GSMADYFHRATLDPDGVFRQYVYPKKANAGILGGETWT 300

Query: 301 KVWGAMRDPC------LVNTVCGLNGLC--KSPDNDTVTCDCLPGFVHLDPADAWKGCRP 360
            V    ++ C      + + VCG N  C      N   +C C P +   D    +KGC+ 
Sbjct: 301 AVSMQPQNICHAIVSDVGSGVCGFNSYCTFDGTRNQIASCQCPPWYKFFDEQKKYKGCKQ 360

Query: 361 ETVINYCMGDSG---KNFTLQVIEDVDIDLPPESEPFSDLVRMFNVDFESCKRAIMEDCY 420
           +   + C  D       F L+ I  VD        P SD  +   +  + C R  + DC+
Sbjct: 361 DFQPHSCDLDEATALAQFELRPIYGVD-------WPLSDYEKYEPIGQDDCGRLCVIDCF 420

Query: 421 VMAATW--KNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNSSEVHDGKNENNFNYR 480
              A +    STC KK+ PL NG   +  + T  L++VP    + S +    N+   N +
Sbjct: 421 CAMAVYNQSTSTCWKKKLPLSNGNMADYVQRT-VLLKVPSSNSSQSMISTSSNKWKRNRK 480

Query: 481 K-VLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNF---LSASAIGINFREFTFQELV 540
             VL    I+   +   F  + +F   T  R+  +KN     ++S   +  + FT++EL 
Sbjct: 481 HWVLGSSLILGTSILVNFALISIFLFGTYCRIATKKNIPLSQASSKSQLPLKTFTYKELE 540

Query: 541 DATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEFVTELRIIGRTYH 600
            AT GF +ILG G+S  V +G L  D +   IAVK +DK+   TE EF+ E+  IG+T+H
Sbjct: 541 KATAGFHEILGAGASGVVYKGQLE-DELKTNIAVKKIDKLQPETEKEFMVEVETIGQTFH 600

Query: 601 TNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLFDNGENTIKISNC 660
            NLVRLLG+C E  ++ LLVYE M NG L+R LFDN                     S  
Sbjct: 601 KNLVRLLGFCNEGAER-LLVYEFMTNGPLNRLLFDN---------------------SRP 660

Query: 661 NWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIADFGISKLLKKDQ 720
           +W  RV IA G+ARGL YLH+EC  QIIHCD+KPQN+LLD N  AKI+DFG++KLL  +Q
Sbjct: 661 HWNTRVHIALGVARGLLYLHDECSKQIIHCDIKPQNILLDDNLVAKISDFGLAKLLLTNQ 720

Query: 721 TRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIELDRVEEESEEED 780
           TRTNT  RGT GY+APEW +   ++ KVDVYS+GV+LLE++CCRR++EL+ V    +EE 
Sbjct: 721 TRTNTGIRGTRGYVAPEWFKNIGISTKVDVYSFGVILLELVCCRRNVELEVV----DEEQ 780

Query: 781 LVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDASQRPSMKKVTQM 806
            +++ W      +G ++ +V    E + + ++ +R   V LWC+  D S RP+M KVTQM
Sbjct: 781 TIVTYWANDCYRSGRIDLLVEGDDEAIYNIKKVERFVTVALWCLQEDPSMRPNMLKVTQM 803

BLAST of Bhi01G000601 vs. ExPASy Swiss-Prot
Match: A0A075F7E9 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 OS=Oryza sativa subsp. indica OX=39946 GN=LECRK1 PE=1 SV=2)

HSP 1 Score: 471.9 bits (1213), Expect = 1.5e-131
Identity = 307/853 (35.99%), Postives = 445/853 (52.17%), Query Frame = 0

Query: 1   MASMIFLSFVLLNFYKCLAQNGSPISRGSSIA-AGSNHTWLSPSGDFAFGFYRLPNN--L 60
           M +++    +L       AQ    I+ GS++A  G   +WLSPSGDFAFGF  +  N   
Sbjct: 1   MVALLLFPMLLQLLSPTCAQTQKNITLGSTLAPQGPASSWLSPSGDFAFGFRPVEGNTSF 60

Query: 61  YLVGIWFDKI-PQTLVWSA-NRDN-----PAPPNSLVKLNRTGQFVLSFPNGIIIQPIFK 120
           YL+ +WF+KI  +T+VW A N D        P +S ++L   G   L   +G   Q  + 
Sbjct: 61  YLIAVWFNKISDKTVVWYAKNTDQDPSIVEVPSDSFLQLTNDGALSLKDRSG---QEGWN 120

Query: 121 EQQAPASSGQMQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDK---KMFSAK-NI 180
            Q    +   M+D GNFVL  ++     Q+FD P+DT+LP Q++  +K   K   A+ +I
Sbjct: 121 PQVTGVAYASMRDTGNFVLLGADGTTKWQTFDMPSDTILPTQVIPCNKTRNKSLRARLDI 180

Query: 181 SDFSTGNFMLQMQKDGNLVL--------SNYHFSNVGYWFTIATNIENTVLVFDKSSALM 240
            D+S+G F+L +Q DGNL L        S Y      YW T  T    + LVF ++  + 
Sbjct: 181 DDYSSGRFLLDVQTDGNLALYLVAVPSGSKYQ----QYWSTDTTG-NGSELVFSETGKVY 240

Query: 241 Y-LTNRINPNGQSLRNLTVNVPAPVGDYYHRATINVHGDFRQYVYPKS------NGSEWI 300
           + LT+    N  S   +       + DY+HRAT++  G FRQYVYPK        G  W 
Sbjct: 241 FALTDGTQINISSDAGI-----GSMADYFHRATLDPDGVFRQYVYPKKANAGILGGETWT 300

Query: 301 KVWGAMRDPC------LVNTVCGLNGLC--KSPDNDTVTCDCLPGFVHLDPADAWKGCRP 360
            +    ++ C      + + VCG N  C      N   +C C P +   D    +KGC+ 
Sbjct: 301 ALSMQPQNICHAIVSDVGSGVCGFNSYCTFDGTRNQIASCQCPPWYKFFDEQKKYKGCKQ 360

Query: 361 ETVINYCMGDSG---KNFTLQVIEDVDIDLPPESEPFSDLVRMFNVDFESCKRAIMEDCY 420
           +   + C  +       F L+ I  VD        P SD  +   +  + C R  + +C+
Sbjct: 361 DFQPHSCDLEEATALAQFELRPIYGVD-------WPLSDYEKYEPIGQDDCGRLCVIECF 420

Query: 421 VMAATW--KNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNSSEVHDGKNENNFNYR 480
              A +    STC KK+ PL NG   +  + T  L++VP    NSS+     + N +   
Sbjct: 421 CAMAVYNQSTSTCWKKKLPLSNGNMADYVQRT-VLLKVP--SSNSSQFMISTSSNKWKRN 480

Query: 481 K---VLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNF---LSASAIGINFREFTFQE 540
           +   VL    I+   +   F  + +F   T  R+  +KN     ++S   +  + FT++E
Sbjct: 481 RKHWVLGSSLILGTSILVNFALISIFLFGTYCRITTKKNIPLSQASSKSQLPLKTFTYKE 540

Query: 541 LVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEFVTELRIIGRT 600
           L  AT GF +ILG G+S  V +G L  D +   IAVK + K+   TE EF+ E+  IG+T
Sbjct: 541 LEKATAGFHEILGAGASGVVYKGQLE-DELKTNIAVKTIHKLQPETEKEFMVEVETIGQT 600

Query: 601 YHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLFDNGENTIKIS 660
           +H NLVRLLG+C E + + LLVYE M NG L+R LFDN                     S
Sbjct: 601 FHKNLVRLLGFCNE-RAERLLVYEFMTNGPLNRLLFDN---------------------S 660

Query: 661 NCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIADFGISKLLKK 720
             +W  RV IA G+ARG  YLH+EC  QIIHCD+KPQN+LLD N  AKI+DFG++KLL  
Sbjct: 661 RPHWNTRVHIALGVARGFLYLHDECSKQIIHCDIKPQNILLDDNLVAKISDFGLAKLLLT 720

Query: 721 DQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIELDRVEEESEE 780
           +QTRT T  RGT GY+APEW +   ++ KVDVYS+GV+LLE++CCRR++EL+ V    +E
Sbjct: 721 NQTRTKTGIRGTRGYVAPEWFKNIGISTKVDVYSFGVILLELVCCRRNVELEVV----DE 780

Query: 781 EDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDASQRPSMKKVT 806
           E  +++ W      +G ++ +V    E + D ++ +R   V LWC+  D S RP+M KVT
Sbjct: 781 EQTIVTYWANDCYRSGRIDLLVEGDDEAIYDIKKVERFVTVALWCLQEDPSMRPNMLKVT 803

BLAST of Bhi01G000601 vs. ExPASy Swiss-Prot
Match: Q25AG3 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 OS=Oryza sativa subsp. indica OX=39946 GN=LECRK3 PE=3 SV=2)

HSP 1 Score: 468.8 bits (1205), Expect = 1.3e-130
Identity = 300/851 (35.25%), Postives = 465/851 (54.64%), Query Frame = 0

Query: 1   MASMIFLSFVLLNFYKCL--AQNGSPISRGSSIA-AGSNHTWLSPSGDFAFGFYRLPNN- 60
           MA ++FL  + L    C   AQ    IS GSS+   G N++W+SPS DFAFGF  +  N 
Sbjct: 1   MAHLLFLPILQLLLLYCTKSAQAQLNISIGSSLTPQGVNNSWISPSADFAFGFRAVDGNS 60

Query: 61  -LYLVGIWFDKI-PQTLVW-----SANRDNPAP----PNSLVKLNRTGQFVLSFPNGIII 120
             YL+ +WF+KI  +T+VW     S  +D+  P      S++KL   G   L  P+G   
Sbjct: 61  SSYLLAVWFNKIADKTVVWYARTSSNGKDDTIPVQVQSGSVLKL-ADGALSLRDPSG--- 120

Query: 121 QPIFKEQQAPASSGQMQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKN 180
             ++  Q       +M D GNF L  ++     +SF  P+DT+LP Q+L +   + S   
Sbjct: 121 NEVWNPQVTDVGYARMLDTGNFRLLGTDGATKWESFGDPSDTILPTQVLSLGTALHSRLL 180

Query: 181 ISDFSTGNFMLQMQKDGNLVL------SNYHFSNVGYWFTIATNIEN-TVLVFDKSSALM 240
            +D+S G F L++Q+DGNLV+      S Y +    YW   +  ++N + LVF+++  + 
Sbjct: 181 ATDYSNGRFQLKVQRDGNLVMYPDAVPSGYLYD--PYW--ASNTVDNGSQLVFNETGRIY 240

Query: 241 YLTNRINPNGQSLRNLTVNVPAPVGDYYHRATINVHGDFRQYVYPKSNGSE--WIKVWGA 300
           +       NG  + N+T      +GD++HRAT++  G FRQYVYPK+  +   W + W A
Sbjct: 241 FTI----INGSQV-NITSAGVDSMGDFFHRATLDTDGVFRQYVYPKNIHARPLWPEQWTA 300

Query: 301 MR----------DPCLVNTVCGLNGLCK-SPDNDTVTCDCLPGFVHLDPADAWKGCRPET 360
           +              + +  CG N  C      +T +C C   +  +D    +KGCRP+ 
Sbjct: 301 VDVLPENICQSIQTMVGSGACGFNSYCTIDGTKNTTSCLCPQNYKFIDDKRKYKGCRPDF 360

Query: 361 VINYCMGDSGK---NFTLQVIEDVDIDLPPESEPFSDLVRMFNVDFESCKRAIMEDCYVM 420
               C  D       + +  I+ VD        P SD  +   +D   C+R  + DC+  
Sbjct: 361 EPQNCDLDETTAMLQYDMAPIDRVD-------WPLSDYEQYNPIDQTECRRLCVIDCFCA 420

Query: 421 AATW--KNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNSSEVHDGKNENNFNYRKV 480
            A +   +STC KKR PL NG+  ++      LI+VP    + S    G ++   + +  
Sbjct: 421 VAVFDKASSTCWKKRFPLSNGK-MDVNVPRTVLIKVPRSTNSPSVFSSGSSKWKEDKKYW 480

Query: 481 LEIGNIIAG---VLAFCFGAVVVF---YHPTARRLIRRKNFLSASAIGINFREFTFQELV 540
           +   +++ G   ++ F   +V++F      T+R+ I+      ++  G+  + FT+ EL 
Sbjct: 481 ILGSSLLFGSSVLVNFLLISVMLFGTYCSITSRKKIQLSQ--PSNKSGLPPKIFTYSELE 540

Query: 541 DATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEFVTELRIIGRTYH 600
            AT GF+++LG G+S  V +G L  D   + IAVK ++K+ +  + EF+ E++ IG+T+H
Sbjct: 541 KATGGFQEVLGTGASGVVYKGQLQ-DEFGINIAVKKIEKLQQEAQKEFLVEVQTIGQTFH 600

Query: 601 TNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLFDNGENTIKISNC 660
            NLVRLLG+C E  ++ LLVYE M NG+L+ FLF +                     ++ 
Sbjct: 601 RNLVRLLGFCNEGTER-LLVYEFMSNGSLNTFLFSD---------------------THP 660

Query: 661 NWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIADFGISKLLKKDQ 720
           +W+ RV++A G+ARGL YLHEEC  QIIHCD+KPQN+LLD N+AAKI+DFG++KLL  +Q
Sbjct: 661 HWSLRVQVALGVARGLLYLHEECNKQIIHCDMKPQNILLDDNFAAKISDFGLAKLLPVNQ 720

Query: 721 TRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIELDRVEEESEEED 780
           T+TNT  RGT GY+APEW +   +T+KVDVYS+GV+LLE++CCR+++EL    E  +EE 
Sbjct: 721 TQTNTGIRGTRGYVAPEWFKNIGITSKVDVYSFGVILLELVCCRKNVEL----EVLDEEQ 780

Query: 781 LVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDASQRPSMKKVTQM 806
            +L+ W       G ++ +V    E + + ++ +R   V LWC+  + S RP+M KVTQM
Sbjct: 781 TILTYWANDCYKCGRIDLLVAGDDEAIFNIKKVERFVAVALWCLQEEPSMRPTMLKVTQM 801

BLAST of Bhi01G000601 vs. ExPASy Swiss-Prot
Match: Q0JEU6 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 OS=Oryza sativa subsp. japonica OX=39947 GN=LECRK3 PE=3 SV=2)

HSP 1 Score: 468.0 bits (1203), Expect = 2.2e-130
Identity = 300/849 (35.34%), Postives = 459/849 (54.06%), Query Frame = 0

Query: 1   MASMIFLSFVLLNFYKCL--AQNGSPISRGSSIA-AGSNHTWLSPSGDFAFGFYRLPNN- 60
           MA ++FL  + L    C   AQ    IS GSS+   G N++W+SPS DFAFGF  +  N 
Sbjct: 1   MAHLLFLPILQLLLLYCTKSAQAQLNISIGSSLTPQGVNNSWISPSADFAFGFLAVDGNS 60

Query: 61  -LYLVGIWFDKI-PQTLVW-----SANRDNPAP----PNSLVKLNRTGQFVLSFPNGIII 120
             YL+ +WF+KI  +T+VW     S  +D+  P      S++KL   G   L  P+G   
Sbjct: 61  SSYLLAVWFNKIADKTVVWYARTSSNGKDDTIPVQVQSGSVLKL-ADGALSLRDPSG--- 120

Query: 121 QPIFKEQQAPASSGQMQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKN 180
             ++  Q       +M D GNF L  ++     +SF  P+DT+LP Q+L +   + S   
Sbjct: 121 NEVWNPQVTDVGYARMLDTGNFRLLGTDGATKWESFGDPSDTILPTQVLSLGTALHSRLL 180

Query: 181 ISDFSTGNFMLQMQKDGNLVL------SNYHFSNVGYWFTIATNIEN-TVLVFDKSSALM 240
            +D+S G F L++Q+DGNLV+      S Y +    YW   +  ++N + LVF+++  + 
Sbjct: 181 ATDYSNGRFQLKVQRDGNLVMYPDAVPSGYLYD--PYW--ASNTVDNGSQLVFNETGRIY 240

Query: 241 YLTNRINPNGQSLRNLTVNVPAPVGDYYHRATINVHGDFRQYVYPKSNGSE--WIKVWGA 300
           +       NG  + N+T      +GD++HRAT++  G FRQYVYPK+  +   W + W A
Sbjct: 241 FTI----INGSQV-NITSAGVDSMGDFFHRATLDTDGVFRQYVYPKNIHARPLWPEQWTA 300

Query: 301 MR----------DPCLVNTVCGLNGLCK-SPDNDTVTCDCLPGFVHLDPADAWKGCRPET 360
           +              + +  CG N  C      +T +C C   +  +D    +KGCRP+ 
Sbjct: 301 VDVLPENICQSIQTMVGSGACGFNSYCTIDGTKNTTSCLCPQNYKFIDDKRKYKGCRPDF 360

Query: 361 VINYCMGDSGK---NFTLQVIEDVDIDLPPESEPFSDLVRMFNVDFESCKRAIMEDCYVM 420
               C  D       + +  I+ VD        P SD  +   +D   C+R  + DC+  
Sbjct: 361 EPQNCDLDETTAMLQYDMAPIDRVD-------WPLSDYEQYNPIDQTECRRLCVTDCFCA 420

Query: 421 AATW--KNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNSSEVHDGKNENNFNYRKV 480
            A +   +STC KKR PL NG+  ++      LI+VP    + S    G ++   + +  
Sbjct: 421 VAVFDKASSTCWKKRFPLSNGK-MDVNVPRTVLIKVPRSTNSPSVFSSGSSKWKEDQKYW 480

Query: 481 LEIGNIIAG--VLAFCFGAVVVFYHPTARRLIRRKNFLS--ASAIGINFREFTFQELVDA 540
           +   +++ G  VL       V+ +        R+K  LS  ++  G+  + FT+ EL  A
Sbjct: 481 ILGSSLLFGSSVLVNFLLISVMLFGTYCSITSRKKTQLSQPSNNSGLPPKIFTYSELEKA 540

Query: 541 TDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEFVTELRIIGRTYHTN 600
           T GF+++LG G+S  V +G L  D     IAVK ++K+ +  + EF+ E++ IG+T+H N
Sbjct: 541 TGGFQEVLGTGASGVVYKGQLQ-DEFGTNIAVKKIEKLQQEAQKEFLVEVQTIGQTFHRN 600

Query: 601 LVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLFDNGENTIKISNCNW 660
           LVRLLG+C E  ++ LLVYE M NG+L+ FLF +                     ++ +W
Sbjct: 601 LVRLLGFCNEGTER-LLVYEFMSNGSLNTFLFSD---------------------THPHW 660

Query: 661 TQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIADFGISKLLKKDQTR 720
           + RV++A G+ARGL YLHEEC  QIIHCD+KPQN+LLD N+ AKI+DFG++KLL  +QT+
Sbjct: 661 SLRVQVALGVARGLLYLHEECNKQIIHCDMKPQNILLDDNFVAKISDFGLAKLLPVNQTQ 720

Query: 721 TNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIELDRVEEESEEEDLV 780
           TNT  RGT GY+APEW +   +T+KVDVYS+GV+LLE++CCR+++EL    E  +EE  +
Sbjct: 721 TNTGIRGTRGYVAPEWFKNIGITSKVDVYSFGVILLELVCCRKNVEL----EVLDEEQTI 780

Query: 781 LSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDASQRPSMKKVTQMLE 806
           L+ W       G ++ +V    E + + ++ +R   V LWC+  + S RP+M KVTQML+
Sbjct: 781 LTYWANDCYKCGRIDLLVAGDDEAIFNIKKVERFVAVALWCLQEEPSMRPTMLKVTQMLD 801

BLAST of Bhi01G000601 vs. ExPASy Swiss-Prot
Match: Q25AG2 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK4 OS=Oryza sativa subsp. indica OX=39946 GN=LECRK4 PE=3 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 2.8e-130
Identity = 310/844 (36.73%), Postives = 452/844 (53.55%), Query Frame = 0

Query: 1   MASMIFL----SFVLLNFYKCLAQNGSPISRGSSIAA-GSNHTWLSPSGDFAFGFYRLPN 60
           MA  +FL      VLL+     AQN   IS G+S+   G N+ WLSPSGDFAFGF  +  
Sbjct: 1   MAPPLFLLSLQLLVLLSSPSAQAQN---ISLGTSLTTQGPNNAWLSPSGDFAFGFRPIDG 60

Query: 61  N--LYLVGIWFDKI-PQTLVWSANRDN------PAPPNSLVKLNRTGQFVLSFPNGIIIQ 120
           N   YL+ IWF+KI  +T  W A            P  S+++   TG   L  P     +
Sbjct: 61  NSSFYLLAIWFNKISDKTATWYAKTSEQEPQPIQVPSGSILQFTSTGVLSLRDPTN---R 120

Query: 121 PIFKEQQAPASSGQMQDDGNFVLKDSNFVAVG-QSFDSPTDTLLPGQILGVDKKMFSAKN 180
            ++      A    M D GNFV+  +    +  ++F +PTDT+L  Q L    K+ S   
Sbjct: 121 EVWNPGATGAPYASMLDTGNFVIAAAGGSTISWETFKNPTDTILVTQALSPGMKLRSRLL 180

Query: 181 ISDFSTGNFMLQMQ-KDGNLVLSNYHFSNV--GYWFT-IATNIENTV--LVFDKSSALMY 240
            +D+S G F+L M+ +   L        N+   YW T I  N+ N V  LVF+ ++  +Y
Sbjct: 181 TTDYSNGRFLLNMETQRAALYTMAVPSGNLYDPYWSTPIDENVTNQVTNLVFN-TTGRIY 240

Query: 241 LTNRINPNGQSLRNLTVNVPAPVGDYYHRATINVHGDFRQYVYPK---SNGSEWIKVWGA 300
           ++ +   NG    N+T  V   + DYYHRAT++  G FRQYVYPK   S    W  V   
Sbjct: 241 VSMK---NGTQF-NMTSGVIRSMEDYYHRATLDPDGVFRQYVYPKKPSSMSQAWTAVSIQ 300

Query: 301 MRDPCLVNT-----VCGLNGLCK-SPDNDTVTCDCLPGFVHLDPADAWKGCRPETVINYC 360
             + C   T      CG N  C     N+  +C C   +   D    ++GCRP+  +  C
Sbjct: 301 PENICNAQTKVGSGTCGFNSYCMFDGSNNQTSCVCPEQYSFFDEVRKYRGCRPDFELQSC 360

Query: 361 MGD---SGKNFTLQVIEDVDIDLPPESEPFSDLVRMFNVDFESCKRAIMEDCYVMAATWK 420
             D   S   +   ++ +VD        P +D      +D + C+R  + DC+   A + 
Sbjct: 361 DLDEAASMAQYEFNLVNNVD-------WPQADYEWYTPIDMDECRRLCLIDCFCAVAVFH 420

Query: 421 NSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNSSEVHDGKNENNFNYRKVLEIGN-- 480
            +TC KK+ PL NG   +  + T  LI+VP    NSS+    K+    + +K+  +G+  
Sbjct: 421 ENTCWKKKLPLSNGIMGSGVQRT-VLIKVPK--SNSSQPELRKSRKWKSDKKLWILGSSL 480

Query: 481 IIAG--VLAFCFGAVVVF--YHPTARRLIRRKNFLSASAIGINFREFTFQELVDATDGFK 540
           ++ G  +  F   +V++F  Y    R+ +  +    +   G+  + F++ EL  ATDGFK
Sbjct: 481 LLGGSVIANFALSSVLLFGTYCTITRKDV--QPLQPSRDPGLPLKAFSYAELEKATDGFK 540

Query: 541 KILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEFVTELRIIGRTYHTNLVRLL 600
           ++LG G+S  V +G L  D +   IAVK +DK+   TE EF  E++ IGRTYH NLVR+L
Sbjct: 541 EVLGTGASGIVYKGQLQ-DELGTYIAVKKIDKIQHETEKEFAVEVQTIGRTYHKNLVRML 600

Query: 601 GYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLFDNGENTIKISNCNWTQRVE 660
           G+C E  ++ LLVYE M NG+L+RFLF     +                     W+ RV+
Sbjct: 601 GFCNEGTER-LLVYEFMVNGSLNRFLFSGVRPL---------------------WSLRVQ 660

Query: 661 IAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIADFGISKLLKKDQTRTNTEA 720
           +A G+ARGL YLHEEC TQIIHCD+KPQN+LLD N+ AKI+DFG++KLL+ +QT+T T  
Sbjct: 661 LALGVARGLLYLHEECSTQIIHCDIKPQNILLDDNFIAKISDFGLAKLLRTNQTQTYTGI 720

Query: 721 RGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIELDRVEEESEEEDLVLSNWV 780
           RGT GY+APEW +   +TAKVDVYS+GV+LLE+ICCR+++E+    E +EEE  +L+ W 
Sbjct: 721 RGTRGYVAPEWFKNVGITAKVDVYSFGVILLELICCRQNVEM----EAAEEEQSILTYWA 780

Query: 781 LSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDASQRPSMKKVTQMLEGTAEV 806
                 G ++ +V    E  ++ ++ +R   V LWC+  + + RPS+ KVTQML+G   +
Sbjct: 781 NDCYRCGRVDLLVDGDDEAKLNIKKVERFVAVALWCLQEEPTMRPSILKVTQMLDGADAI 794

BLAST of Bhi01G000601 vs. NCBI nr
Match: XP_038896440.1 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 [Benincasa hispida])

HSP 1 Score: 1660.6 bits (4299), Expect = 0.0e+00
Identity = 810/810 (100.00%), Postives = 810/810 (100.00%), Query Frame = 0

Query: 1   MASMIFLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLV 60
           MASMIFLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLV
Sbjct: 1   MASMIFLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLV 60

Query: 61  GIWFDKIPQTLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQ 120
           GIWFDKIPQTLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQ
Sbjct: 61  GIWFDKIPQTLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQ 120

Query: 121 MQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQK 180
           MQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQK
Sbjct: 121 MQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQK 180

Query: 181 DGNLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPA 240
           DGNLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPA
Sbjct: 181 DGNLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPA 240

Query: 241 PVGDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDT 300
           PVGDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDT
Sbjct: 241 PVGDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDT 300

Query: 301 VTCDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDL 360
           VTCDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDL
Sbjct: 301 VTCDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDL 360

Query: 361 VRMFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFF 420
           VRMFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFF
Sbjct: 361 VRMFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFF 420

Query: 421 KNSSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASA 480
           KNSSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASA
Sbjct: 421 KNSSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASA 480

Query: 481 IGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTET 540
           IGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTET
Sbjct: 481 IGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTET 540

Query: 541 EFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRF 600
           EFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRF
Sbjct: 541 EFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRF 600

Query: 601 LFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAK 660
           LFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAK
Sbjct: 601 LFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAK 660

Query: 661 IADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRH 720
           IADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRH
Sbjct: 661 IADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRH 720

Query: 721 IELDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHP 780
           IELDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHP
Sbjct: 721 IELDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHP 780

Query: 781 DASQRPSMKKVTQMLEGTAEVGTPPLLLCS 811
           DASQRPSMKKVTQMLEGTAEVGTPPLLLCS
Sbjct: 781 DASQRPSMKKVTQMLEGTAEVGTPPLLLCS 810

BLAST of Bhi01G000601 vs. NCBI nr
Match: XP_022955921.1 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK4 [Cucurbita moschata] >KAG6582292.1 G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 583/806 (72.33%), Postives = 646/806 (80.15%), Query Frame = 0

Query: 4   MIFLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLVGIW 63
           ++ LSF+L+NF++CLAQ GS IS GSSI AGS H+W+SP GDFAFGFY L N+LYL GIW
Sbjct: 5   LMLLSFLLMNFHECLAQTGSAISPGSSITAGSTHSWVSPLGDFAFGFYHLSNDLYLAGIW 64

Query: 64  FDKIPQ-TLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQ 123
           FDKIP+  LVWSANRDNPAP +S+V+LN TG F +  P G II   F EQ+ PASSGQMQ
Sbjct: 65  FDKIPEKILVWSANRDNPAPLDSVVQLNNTGHFEILSPTGSIIPSTFTEQKTPASSGQMQ 124

Query: 124 DDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQKDG 183
           DDGN VLK++N  AV QSFD PTDTLLPGQ+LGVDKKMFSA+  SDFSTGNFMLQMQ DG
Sbjct: 125 DDGNLVLKNANPEAVWQSFDWPTDTLLPGQVLGVDKKMFSARTSSDFSTGNFMLQMQSDG 184

Query: 184 NLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPAPV 243
           NLVLSNY FSN+GYWFTIAT + NTVL+F+ +SA M+LTN  +P GQ  RNLTVN  AP+
Sbjct: 185 NLVLSNYLFSNIGYWFTIATEVANTVLMFN-NSASMFLTNGTSPIGQIFRNLTVNDQAPI 244

Query: 244 GDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVT 303
            DYYHRATI VHGDFRQY++ K++ +EW K+WGAM DPCLVNTVCGLNGLC S DNDTVT
Sbjct: 245 KDYYHRATIGVHGDFRQYIHHKTDRNEWKKIWGAMSDPCLVNTVCGLNGLCISSDNDTVT 304

Query: 304 CDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDLVR 363
           CDCLPGFVHLDP DA KGCRP+TV NY   D GK F +QVI DVDID PP +  FS L  
Sbjct: 305 CDCLPGFVHLDPTDAMKGCRPKTVDNYRSEDYGKTFEIQVINDVDIDFPPGAGQFSVLAT 364

Query: 364 MFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKN 423
             +VD E CK+AI+ D Y MAAT    TCL KRTPLMN RNT+ TKG +TLI+VP    N
Sbjct: 365 TNDVDVEGCKQAILGDRYAMAATLNGRTCLSKRTPLMNARNTSNTKGLRTLIKVP--SGN 424

Query: 424 SSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASAIG 483
           S    + K ++   YRK LEIGNIIAGVLAFCFG V +F HP   RL+RRK   SASAIG
Sbjct: 425 SPMGLNNKPKDKSKYRKFLEIGNIIAGVLAFCFGVVALFSHPITGRLLRRKQRSSASAIG 484

Query: 484 INFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEF 543
           INFREFT+QEL DATDGF +ILG+GSS KV  G+LHIDG++VEIAVK+LDKM ERTE EF
Sbjct: 485 INFREFTYQELDDATDGFHRILGRGSSGKVFSGDLHIDGVEVEIAVKLLDKMHERTENEF 544

Query: 544 VTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLF 603
           VTEL IIGRTYH NLVRLLGYCIE + Q LLVYELMP GALS FLF +GEN         
Sbjct: 545 VTELTIIGRTYHKNLVRLLGYCIEKENQFLLVYELMPRGALSGFLFGSGENP-------- 604

Query: 604 DNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIA 663
                       NW QRVEIA GIARGLAYLHE CETQIIHCDVKPQNVLLDANY  KIA
Sbjct: 605 ------------NWAQRVEIALGIARGLAYLHEGCETQIIHCDVKPQNVLLDANYTTKIA 664

Query: 664 DFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIE 723
           DFGISKLL KDQTRTNTEARGT GYMAPEWLRGAPVTAKVDV+SYGVMLLEIIC RR++E
Sbjct: 665 DFGISKLLMKDQTRTNTEARGTFGYMAPEWLRGAPVTAKVDVFSYGVMLLEIICRRRNVE 724

Query: 724 LDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDA 783
           LDRVEEESEEEDLVLSNWVLS AAAG LETVVGD+ E L D +RF+RMAMVGLWCIHPDA
Sbjct: 725 LDRVEEESEEEDLVLSNWVLSCAAAGKLETVVGDEPETLRDLDRFERMAMVGLWCIHPDA 784

Query: 784 SQRPSMKKVTQMLEGTAEVGTPPLLL 809
           SQRPSMKKVTQMLEGT +VGTPP LL
Sbjct: 785 SQRPSMKKVTQMLEGTDDVGTPPQLL 787

BLAST of Bhi01G000601 vs. NCBI nr
Match: KAG7018695.1 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 583/806 (72.33%), Postives = 645/806 (80.02%), Query Frame = 0

Query: 4    MIFLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLVGIW 63
            ++ LSF+L+NF++CLAQ GS IS GSSI AGS H+W+SP GDFAFGFY L N LYL GIW
Sbjct: 498  LMLLSFLLMNFHECLAQTGSAISPGSSITAGSTHSWVSPLGDFAFGFYHLSNYLYLAGIW 557

Query: 64   FDKIPQ-TLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQ 123
            FDKIP+  LVWSANRDNPAP +S+V+LN TG F +  P G II   F EQ+ PASSGQMQ
Sbjct: 558  FDKIPEKILVWSANRDNPAPLDSVVQLNNTGHFEILSPTGSIIPSTFTEQKTPASSGQMQ 617

Query: 124  DDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQKDG 183
            DDGN VLK++N  AV QSFD PTDTLLPGQ+LGVDKKMFSA+  SDFSTGNFMLQMQ DG
Sbjct: 618  DDGNLVLKNANPEAVWQSFDWPTDTLLPGQVLGVDKKMFSARTSSDFSTGNFMLQMQSDG 677

Query: 184  NLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPAPV 243
            NLVLSNY FSN+GYWFTIAT + NTVL+FD +SA M+LTN  +P GQ  RNLTVN  AP+
Sbjct: 678  NLVLSNYLFSNIGYWFTIATEVPNTVLMFD-NSASMFLTNGTSPIGQIFRNLTVNDQAPI 737

Query: 244  GDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVT 303
             DYYHRATI VHGDFRQY++ K++ +EW K+WGAM DPCLVNTVCGLNGLC S DNDTVT
Sbjct: 738  KDYYHRATIGVHGDFRQYIHHKTDRNEWKKIWGAMSDPCLVNTVCGLNGLCISSDNDTVT 797

Query: 304  CDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDLVR 363
            CDCLPGFVHLDP DA KGCRP+TV NY   D GK F +QVI DVDID PP +  FS L  
Sbjct: 798  CDCLPGFVHLDPTDAMKGCRPKTVDNYRSEDYGKTFEIQVINDVDIDFPPGAGQFSVLAT 857

Query: 364  MFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKN 423
              +VD E CK+AI+ D Y MAAT    TCL KRTPLMN RNT+ TKG +TLI+VP    N
Sbjct: 858  TNDVDVEGCKQAILGDRYAMAATLNGRTCLSKRTPLMNARNTSNTKGLRTLIKVP--SGN 917

Query: 424  SSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASAIG 483
            S    + K ++   YRK LEIGNIIAGVLAFCFG V +F HP   RL+RRK   SASAIG
Sbjct: 918  SPMGLNNKPKDKSKYRKFLEIGNIIAGVLAFCFGVVALFSHPITGRLLRRKQRSSASAIG 977

Query: 484  INFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEF 543
            INFREFT+QEL DATDGF +ILG+GSS KV  G+LHIDG++VEIAVK+LDKM +RTE EF
Sbjct: 978  INFREFTYQELDDATDGFHRILGRGSSGKVFSGDLHIDGVEVEIAVKLLDKMHDRTENEF 1037

Query: 544  VTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLF 603
            VTEL IIGRTYH NLVRLLGYCIE + Q LLVYELMP GALS FLF +GEN         
Sbjct: 1038 VTELTIIGRTYHKNLVRLLGYCIEKENQFLLVYELMPRGALSGFLFGSGENP-------- 1097

Query: 604  DNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIA 663
                        NW QRVEIA GIARGLAYLHE CETQIIHCDVKPQNVLLDANY  KIA
Sbjct: 1098 ------------NWAQRVEIALGIARGLAYLHEGCETQIIHCDVKPQNVLLDANYTTKIA 1157

Query: 664  DFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIE 723
            DFGISKLL KDQTRTNTEARGT GYMAPEWLRGAPVTAKVDV+SYGVMLLEIIC RR++E
Sbjct: 1158 DFGISKLLMKDQTRTNTEARGTFGYMAPEWLRGAPVTAKVDVFSYGVMLLEIICRRRNVE 1217

Query: 724  LDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDA 783
            LDRVEEESEEEDLVLSNWVLS AAAG LETVVGD+ E L D +RF+RMAMVGLWCIHPDA
Sbjct: 1218 LDRVEEESEEEDLVLSNWVLSCAAAGKLETVVGDEPETLRDLDRFERMAMVGLWCIHPDA 1277

Query: 784  SQRPSMKKVTQMLEGTAEVGTPPLLL 809
            SQRPSMKKVTQMLEGT +VGTPP LL
Sbjct: 1278 SQRPSMKKVTQMLEGTDDVGTPPQLL 1280

BLAST of Bhi01G000601 vs. NCBI nr
Match: XP_023526919.1 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 558/806 (69.23%), Postives = 621/806 (77.05%), Query Frame = 0

Query: 4   MIFLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLVGIW 63
           ++ LSF+L+NF++CLAQ GS IS GSSI AGS H+W+SP GDFAFGFY L N+LYL GIW
Sbjct: 5   LMLLSFLLMNFHQCLAQTGSAISPGSSITAGSTHSWVSPLGDFAFGFYHLSNDLYLAGIW 64

Query: 64  FDKIPQ-TLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQ 123
           FDKIP+  LVWSANRDNPAP +S+V+LN TG F +  P G II   F EQ+ PASSGQMQ
Sbjct: 65  FDKIPEKILVWSANRDNPAPLDSVVQLNNTGHFEILSPTGSIIPSTFTEQKTPASSGQMQ 124

Query: 124 DDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQKDG 183
           DDGN VLK++N  AV QSFD PTDTLLPGQ+LGVDKKMFSA+  SDFSTGNFMLQMQ DG
Sbjct: 125 DDGNLVLKNANPEAVWQSFDWPTDTLLPGQVLGVDKKMFSARTNSDFSTGNFMLQMQSDG 184

Query: 184 NLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPAPV 243
           NLVLSNY FSN+GYWFTIAT + NTVL+FD +SA M+LTN  +P GQ  RNLTVN  AP+
Sbjct: 185 NLVLSNYLFSNIGYWFTIATEVPNTVLMFD-NSASMFLTNGTSPIGQIFRNLTVNDQAPI 244

Query: 244 GDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVT 303
            DYYHRATI VHGDFRQY++ K++ +EW K+WGAM DPCLVNTVCGLNGLC S DNDTVT
Sbjct: 245 KDYYHRATIGVHGDFRQYIHHKTDRNEWKKIWGAMSDPCLVNTVCGLNGLCISSDNDTVT 304

Query: 304 CDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDLVR 363
           CDCLPGFVHLDP DA KGCRP+TV NY   D GK F +QVI DVDID PP +  FS L  
Sbjct: 305 CDCLPGFVHLDPTDAMKGCRPKTVDNYHSEDYGKTFEIQVINDVDIDFPPGAGQFSVLAT 364

Query: 364 MFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKN 423
             +VD E CK+ ++                         +N+N +            F+ 
Sbjct: 365 TNDVDVEGCKQDMI-------------------------KNSNQSA-----------FRK 424

Query: 424 SSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASAIG 483
            S+    + +    YRK LEIGNIIAGVLAFCFG V +F HP   RL+RRK   SASAIG
Sbjct: 425 QSDGFKQQTKGKSKYRKFLEIGNIIAGVLAFCFGVVALFSHPITGRLLRRKQRSSASAIG 484

Query: 484 INFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEF 543
           INFREFT+QEL DATDGF +ILG+GSS KV RG+LHIDG++VEIAVK+LDKM ERTE EF
Sbjct: 485 INFREFTYQELDDATDGFHRILGRGSSGKVFRGDLHIDGVEVEIAVKLLDKMHERTENEF 544

Query: 544 VTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLF 603
           VTEL IIGRTYH NLVRLLGYCIE   Q LLVYELMP GALS FLF +GEN         
Sbjct: 545 VTELTIIGRTYHKNLVRLLGYCIEKDNQFLLVYELMPRGALSGFLFGSGENP-------- 604

Query: 604 DNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIA 663
                       NW QRVEIA GIARGLAYLHE CETQIIHCDVKPQNVLLDANY  KIA
Sbjct: 605 ------------NWAQRVEIALGIARGLAYLHEGCETQIIHCDVKPQNVLLDANYTTKIA 664

Query: 664 DFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIE 723
           DFGISKLL KDQTRTNTEARGT GYMAPEWLRGAPVTAKVDV+SYGVMLLEIIC RR++E
Sbjct: 665 DFGISKLLMKDQTRTNTEARGTFGYMAPEWLRGAPVTAKVDVFSYGVMLLEIICRRRNVE 724

Query: 724 LDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDA 783
           LDRVEEESEEEDLVLSNWVLS AAAG LETVVGD+ E L D +RF+RMAMVGLWCIHPDA
Sbjct: 725 LDRVEEESEEEDLVLSNWVLSCAAAGKLETVVGDEPETLRDLDRFERMAMVGLWCIHPDA 753

Query: 784 SQRPSMKKVTQMLEGTAEVGTPPLLL 809
           SQRPSMKKVTQMLEGTA+VGTPP LL
Sbjct: 785 SQRPSMKKVTQMLEGTADVGTPPQLL 753

BLAST of Bhi01G000601 vs. NCBI nr
Match: XP_021665706.1 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 [Hevea brasiliensis])

HSP 1 Score: 914.1 bits (2361), Expect = 8.7e-262
Identity = 470/804 (58.46%), Postives = 579/804 (72.01%), Query Frame = 0

Query: 6   FLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLVGIWFD 65
           +L  + LNF    AQ    IS GSSI AG+N +W S SG+FAFGFY L NNLYL+GIWFD
Sbjct: 9   YLLILALNFCGLHAQILPNISLGSSITAGTNDSWRSLSGEFAFGFYPLQNNLYLMGIWFD 68

Query: 66  KIPQ-TLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQDD 125
           KIP+ TLVWSANRD PA   S ++L   GQ  L++ NG  +Q ++    A AS G M+++
Sbjct: 69  KIPERTLVWSANRDTPAEAGSTIRLTFAGQLFLTYSNG-SVQSVY--GGAAASLGFMENN 128

Query: 126 GNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFS-AKNISDFSTGNFMLQMQKDGN 185
           GNFVLKD N   + QSFDSPTDTLLP Q+L   + +FS AK  +D+STGNFML+MQ DGN
Sbjct: 129 GNFVLKDGNSRVIWQSFDSPTDTLLPTQVLTNGQMLFSNAKGTTDYSTGNFMLEMQYDGN 188

Query: 186 LVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPAPVG 245
           LVLS YHFS+ GYWFT  T + N  LVFD S+A +Y+ N  N     + +LT N  AP G
Sbjct: 189 LVLSAYHFSDPGYWFT-GTLVSNDSLVFD-SNASLYIVNSTN---DVIYSLTENFSAPTG 248

Query: 246 DYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVTC 305
           +YYHRATI+  G+F+QYVY KSNGS WI VW A+ +PCLVN VCG+NG+C SPDN+TVTC
Sbjct: 249 EYYHRATIDDMGNFQQYVYHKSNGSGWISVWKAIHEPCLVNAVCGVNGMCSSPDNETVTC 308

Query: 306 DCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDLVRM 365
           +C+PG++ LDP    KGC PETV+NYC   S +NFT+ VI+D D       E F+DL R+
Sbjct: 309 NCIPGYIPLDPNHVSKGCHPETVVNYCADPSMRNFTIAVIDDADFPF----EGFADLDRV 368

Query: 366 FNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNS 425
            NVD E CK A+M+DCY +AA+  +S C KKR PL+N R +  TKG    ++VPL   N 
Sbjct: 369 LNVDVEGCKVALMDDCYSIAASLVDSRCNKKRMPLLNARKSTSTKGITAFVKVPLKSSNP 428

Query: 426 SEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASAIGI 485
               +G+ +N+FN+R  LEI  I++  LAF FGA  ++YHP  R+ +RRK+  S + IG+
Sbjct: 429 G-TQEGEKKNHFNFRAFLEISLIVSATLAFVFGATAIYYHPGTRKFVRRKH-SSNTTIGV 488

Query: 486 NFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEFV 545
           NFREF + EL +AT+GF K +G+GSS KV  G L +  + +EIAVK L+K  E++E EF+
Sbjct: 489 NFREFKYVELHEATNGFSKTVGRGSSGKVYSGVLSLKNVRIEIAVKKLEKEIEKSEEEFM 548

Query: 546 TELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLFD 605
           TEL+IIGRTYH NLVRLLGYC+EN  Q LLVYELM NG LS  LF+ GE           
Sbjct: 549 TELKIIGRTYHKNLVRLLGYCVEN-NQRLLVYELMANGTLSDLLFEEGERP--------- 608

Query: 606 NGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIAD 665
                      +W  R E+  GI+RGL YLHEECETQIIHCD+KPQNVLLDANY+AKIAD
Sbjct: 609 -----------SWVPRAEMVLGISRGLLYLHEECETQIIHCDLKPQNVLLDANYSAKIAD 668

Query: 666 FGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIEL 725
           FG+SKLL KDQTRT+T  RGT GY+APEWLR APVT+KVDVYS+GV+LLEI+CCRRHIEL
Sbjct: 669 FGLSKLLNKDQTRTDTNVRGTMGYLAPEWLRNAPVTSKVDVYSFGVVLLEILCCRRHIEL 728

Query: 726 DRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDAS 785
           +RVEEESEE+DLVLS+WV+S      LE VV    E+L DF+RF+RMAMVG+WCIHPD  
Sbjct: 729 NRVEEESEEDDLVLSDWVVSCMMKRKLEMVVRHDPEVLSDFKRFERMAMVGIWCIHPDPV 777

Query: 786 QRPSMKKVTQMLEGTAEVGTPPLL 808
            RPSMKKVTQMLEGT+EVG PPLL
Sbjct: 789 LRPSMKKVTQMLEGTSEVGIPPLL 777

BLAST of Bhi01G000601 vs. ExPASy TrEMBL
Match: A0A6J1GV65 (Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111457760 PE=3 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 583/806 (72.33%), Postives = 646/806 (80.15%), Query Frame = 0

Query: 4   MIFLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLVGIW 63
           ++ LSF+L+NF++CLAQ GS IS GSSI AGS H+W+SP GDFAFGFY L N+LYL GIW
Sbjct: 5   LMLLSFLLMNFHECLAQTGSAISPGSSITAGSTHSWVSPLGDFAFGFYHLSNDLYLAGIW 64

Query: 64  FDKIPQ-TLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQ 123
           FDKIP+  LVWSANRDNPAP +S+V+LN TG F +  P G II   F EQ+ PASSGQMQ
Sbjct: 65  FDKIPEKILVWSANRDNPAPLDSVVQLNNTGHFEILSPTGSIIPSTFTEQKTPASSGQMQ 124

Query: 124 DDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKNISDFSTGNFMLQMQKDG 183
           DDGN VLK++N  AV QSFD PTDTLLPGQ+LGVDKKMFSA+  SDFSTGNFMLQMQ DG
Sbjct: 125 DDGNLVLKNANPEAVWQSFDWPTDTLLPGQVLGVDKKMFSARTSSDFSTGNFMLQMQSDG 184

Query: 184 NLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPAPV 243
           NLVLSNY FSN+GYWFTIAT + NTVL+F+ +SA M+LTN  +P GQ  RNLTVN  AP+
Sbjct: 185 NLVLSNYLFSNIGYWFTIATEVANTVLMFN-NSASMFLTNGTSPIGQIFRNLTVNDQAPI 244

Query: 244 GDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVT 303
            DYYHRATI VHGDFRQY++ K++ +EW K+WGAM DPCLVNTVCGLNGLC S DNDTVT
Sbjct: 245 KDYYHRATIGVHGDFRQYIHHKTDRNEWKKIWGAMSDPCLVNTVCGLNGLCISSDNDTVT 304

Query: 304 CDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDLVR 363
           CDCLPGFVHLDP DA KGCRP+TV NY   D GK F +QVI DVDID PP +  FS L  
Sbjct: 305 CDCLPGFVHLDPTDAMKGCRPKTVDNYRSEDYGKTFEIQVINDVDIDFPPGAGQFSVLAT 364

Query: 364 MFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKN 423
             +VD E CK+AI+ D Y MAAT    TCL KRTPLMN RNT+ TKG +TLI+VP    N
Sbjct: 365 TNDVDVEGCKQAILGDRYAMAATLNGRTCLSKRTPLMNARNTSNTKGLRTLIKVP--SGN 424

Query: 424 SSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASAIG 483
           S    + K ++   YRK LEIGNIIAGVLAFCFG V +F HP   RL+RRK   SASAIG
Sbjct: 425 SPMGLNNKPKDKSKYRKFLEIGNIIAGVLAFCFGVVALFSHPITGRLLRRKQRSSASAIG 484

Query: 484 INFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEF 543
           INFREFT+QEL DATDGF +ILG+GSS KV  G+LHIDG++VEIAVK+LDKM ERTE EF
Sbjct: 485 INFREFTYQELDDATDGFHRILGRGSSGKVFSGDLHIDGVEVEIAVKLLDKMHERTENEF 544

Query: 544 VTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLF 603
           VTEL IIGRTYH NLVRLLGYCIE + Q LLVYELMP GALS FLF +GEN         
Sbjct: 545 VTELTIIGRTYHKNLVRLLGYCIEKENQFLLVYELMPRGALSGFLFGSGENP-------- 604

Query: 604 DNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIA 663
                       NW QRVEIA GIARGLAYLHE CETQIIHCDVKPQNVLLDANY  KIA
Sbjct: 605 ------------NWAQRVEIALGIARGLAYLHEGCETQIIHCDVKPQNVLLDANYTTKIA 664

Query: 664 DFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIE 723
           DFGISKLL KDQTRTNTEARGT GYMAPEWLRGAPVTAKVDV+SYGVMLLEIIC RR++E
Sbjct: 665 DFGISKLLMKDQTRTNTEARGTFGYMAPEWLRGAPVTAKVDVFSYGVMLLEIICRRRNVE 724

Query: 724 LDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDA 783
           LDRVEEESEEEDLVLSNWVLS AAAG LETVVGD+ E L D +RF+RMAMVGLWCIHPDA
Sbjct: 725 LDRVEEESEEEDLVLSNWVLSCAAAGKLETVVGDEPETLRDLDRFERMAMVGLWCIHPDA 784

Query: 784 SQRPSMKKVTQMLEGTAEVGTPPLLL 809
           SQRPSMKKVTQMLEGT +VGTPP LL
Sbjct: 785 SQRPSMKKVTQMLEGTDDVGTPPQLL 787

BLAST of Bhi01G000601 vs. ExPASy TrEMBL
Match: A0A5B7C1T6 (Receptor-like serine/threonine-protein kinase OS=Davidia involucrata OX=16924 GN=Din_044259 PE=3 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 7.7e-264
Identity = 476/813 (58.55%), Postives = 586/813 (72.08%), Query Frame = 0

Query: 1   MASMIFLSFVLLNFYKCL----AQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNN 60
           MA+ + L +  + F   L    AQ    IS GS+I AGSN +W S SGDFAFGFY +   
Sbjct: 1   MAATLILPWFFVLFSTLLHVSHAQIPPNISLGSNIIAGSNLSWRSLSGDFAFGFYPVSTG 60

Query: 61  LYLVGIWFDKIPQ-TLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAP 120
           LYLVGIWFDKI +  LVWS NRD+PA   S ++L   GQ  L++ NG  +Q I+    A 
Sbjct: 61  LYLVGIWFDKIQERALVWSPNRDSPAQRGSEIRLTFDGQLRLTYSNG-SVQTIY--SGAA 120

Query: 121 ASSGQMQDDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFSAKN-ISDFSTGNF 180
           AS G MQ+DGNFVL+DSN   + QSFDSPTDTLLPGQ+L   KK+FS  N   DFSTGNF
Sbjct: 121 ASLGFMQNDGNFVLRDSNSGVIWQSFDSPTDTLLPGQVLVRGKKLFSNTNGTVDFSTGNF 180

Query: 181 MLQMQKDGNLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNL 240
           ML+MQ DGNLVLS YHFS+ GYW+T  +N  N  LVF+++SA MYL N    N   +  L
Sbjct: 181 MLEMQFDGNLVLSAYHFSDPGYWYTATSN--NVSLVFNQTSAFMYLVNDTRDN---IYAL 240

Query: 241 TVNVPAPVGDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCK 300
           T NV  PVGDYYHRATI+ +G+F+QYV+ KSNGS W  VW A+ +PC+VN+VCG+NG C 
Sbjct: 241 TKNVTTPVGDYYHRATIDEYGNFQQYVFHKSNGSSWRSVWKAISEPCIVNSVCGVNGFCT 300

Query: 301 SPDNDTVTCDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPES 360
           SPDN+TVTC+CL G++ LDP    KGCRPETV+NYC   S +NFT++VI+D D       
Sbjct: 301 SPDNETVTCNCLQGYLPLDPNSPSKGCRPETVLNYCEDPSARNFTVEVIDDADFPF---- 360

Query: 361 EPFSDLVRMFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLI 420
           + F+DL R+ NVD E CK+A+M+DCY +AA+   STC+KKR PL+N R +N T G K LI
Sbjct: 361 QGFADLSRVRNVDVEGCKKALMDDCYTIAASLDKSTCIKKRMPLLNARKSNSTTGIKALI 420

Query: 421 RVPLFFKNSSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKN 480
           +VP+   NS  + DG  + N N R  L+ G + +G+ AF FGA+ ++YHP ARRLIRR+ 
Sbjct: 421 KVPIKIPNSG-LPDGYKKKNSNTRAHLKAGLLTSGIFAFLFGAIALYYHPAARRLIRRRQ 480

Query: 481 FLSASAIGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKM 540
           F + + IGINFREFT+QEL +AT+GF K LG+GSS KV  G L+++ I +EIAVK L+ +
Sbjct: 481 FPNPTPIGINFREFTYQELREATNGFSKTLGRGSSAKVYSGVLNLNDIQIEIAVKKLEHV 540

Query: 541 TERTETEFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENM 600
            E+ E  F+TEL+IIGRT+H NLVRLLG+CIE+  + LLVYELM NGALS FLF +GE  
Sbjct: 541 VEQGEKGFMTELKIIGRTHHKNLVRLLGFCIEDDHR-LLVYELMKNGALSDFLFKDGERP 600

Query: 601 GALSRFLFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLD 660
                               +W+QR E+A GIARGL YLHEECE+QIIHCD+KPQNVLLD
Sbjct: 601 --------------------SWSQRSEMALGIARGLLYLHEECESQIIHCDIKPQNVLLD 660

Query: 661 ANYAAKIADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEI 720
            NY AKIADFG SKL+ KDQTRT T ARGT GYMAPEWLR AP+T KVD+YS+GVMLLEI
Sbjct: 661 VNYTAKIADFGFSKLMNKDQTRTKTGARGTMGYMAPEWLRNAPITTKVDIYSFGVMLLEI 720

Query: 721 ICCRRHIELDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVG 780
            C RRHIEL RVEEESEEEDL+L+NW LS   +G LE +V   +E+L DF+RF+R+ MVG
Sbjct: 721 SCARRHIELSRVEEESEEEDLILTNWALSCVRSGKLEKLVRHDSEVLSDFKRFERIVMVG 779

Query: 781 LWCIHPDASQRPSMKKVTQMLEGTAEVGTPPLL 808
           LWC+HPDA  RPSMKKVTQMLEGT EV  PPL+
Sbjct: 781 LWCVHPDAILRPSMKKVTQMLEGTMEVEIPPLV 779

BLAST of Bhi01G000601 vs. ExPASy TrEMBL
Match: A0A067KVC5 (Receptor-like serine/threonine-protein kinase OS=Jatropha curcas OX=180498 GN=JCGZ_02159 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 3.9e-260
Identity = 464/807 (57.50%), Postives = 575/807 (71.25%), Query Frame = 0

Query: 4   MIFLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLVGIW 63
           ++ LS  L +F+  +  N   IS GS I AG+N +W SPSG+FAFGFY LPNNL+L+GIW
Sbjct: 10  LVILSLNLCSFHAQILPN---ISLGSGITAGTNDSWRSPSGEFAFGFYSLPNNLFLLGIW 69

Query: 64  FDKIPQ-TLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQ 123
           FDKIP+  LVWSANRD+PA   S+V+L   GQ  L++ NG  +QP++    A AS G M 
Sbjct: 70  FDKIPEGKLVWSANRDSPAEAGSIVRLTFAGQLFLTYSNG-SVQPLY--NGAAASLGFML 129

Query: 124 DDGNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFS-AKNISDFSTGNFMLQMQKD 183
           +DGNFVL+D N   + QSFDSPTDTLLPGQ+L   +K++S AK  +D+STGNFMLQMQ D
Sbjct: 130 NDGNFVLRDGNSEIIWQSFDSPTDTLLPGQVLAKGRKLYSNAKGTADYSTGNFMLQMQFD 189

Query: 184 GNLVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNV-PA 243
           GNLVLS YHFS+ GYW+T  T + N  LVF   +A +YL N  N N   +  L  NV  A
Sbjct: 190 GNLVLSAYHFSDPGYWYT-GTLVNNVSLVF-SPNASVYLVNSTNDN---IYTLNTNVSAA 249

Query: 244 PVGDYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDT 303
            VGDYYHRATI+ HG+F+Q+V+ KSN S W  VW A+ +PC+VN +CG+NG+C SPDN+T
Sbjct: 250 SVGDYYHRATIDDHGNFQQFVHHKSNSSGWTSVWRAIHEPCIVNAICGVNGMCTSPDNET 309

Query: 304 VTCDCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDL 363
             C C+PG++ LDP    KGCRPET +NYC   S +NFT+QVI+D D  L    E F DL
Sbjct: 310 ANCHCIPGYISLDPNQVSKGCRPETTVNYCADPSFRNFTIQVIDDADFPL----EGFQDL 369

Query: 364 VRMFNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFF 423
            R+ NVD E CK A++ DCY +AA+  +S C KKR PL+N R +  TKG K L++VP+  
Sbjct: 370 DRVLNVDVEGCKEALISDCYSLAASLVDSRCNKKRAPLLNARKSTSTKGIKALVKVPMKI 429

Query: 424 KNSSEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASA 483
            N +    G+ +N+FN    L+I  I +  LAF FG + ++YHP  +R IRRK+F +A+ 
Sbjct: 430 SNPAN-PKGEKKNDFNVVAFLKISLIASANLAFLFGVIAIYYHPAFKRFIRRKHFTNATT 489

Query: 484 IGINFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTET 543
           IGI FREF FQEL +AT+GF K LG+GSS KV  G L ++   + +AVK L+K  E++E 
Sbjct: 490 IGIIFREFKFQELHEATNGFNKTLGRGSSGKVYSGILSLNDTRIAVAVKKLEKEIEKSEE 549

Query: 544 EFVTELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRF 603
           EF+TEL+II RT+H NLVRLLG+C+E K Q  LVYELM NGALS  LF +GE        
Sbjct: 550 EFMTELKIISRTHHKNLVRLLGFCVE-KNQHFLVYELMANGALSDLLFGDGERP------ 609

Query: 604 LFDNGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAK 663
                         +W+ R E+  GIARGL YLHEECETQIIHCDVKPQNVL+DANY AK
Sbjct: 610 --------------SWSLRTEMVLGIARGLLYLHEECETQIIHCDVKPQNVLIDANYNAK 669

Query: 664 IADFGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRH 723
           IADFG+SKLL KDQTRTNT  RGT GY+APEWLR APVT+KVDVYS+GVMLLEI+CCRRH
Sbjct: 670 IADFGLSKLLNKDQTRTNTNVRGTMGYLAPEWLRNAPVTSKVDVYSFGVMLLEILCCRRH 729

Query: 724 IELDRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHP 783
           IELDRVEEE + +DLVLS+W +S   +G LE +V    E L DFERF+RMA+VG+WCIHP
Sbjct: 730 IELDRVEEEGDNDDLVLSDWFISCLNSGELEMIVRHDTEALSDFERFERMALVGVWCIHP 779

Query: 784 DASQRPSMKKVTQMLEGTAEVGTPPLL 808
           D   RPSMKKVTQMLEGT EVG PPLL
Sbjct: 790 DPVLRPSMKKVTQMLEGTLEVGIPPLL 779

BLAST of Bhi01G000601 vs. ExPASy TrEMBL
Match: A0A2C9W8L1 (Receptor-like serine/threonine-protein kinase OS=Manihot esculenta OX=3983 GN=MANES_03G180000 PE=3 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 2.0e-259
Identity = 464/804 (57.71%), Postives = 577/804 (71.77%), Query Frame = 0

Query: 6   FLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLVGIWFD 65
           +L  + +N     AQ    IS GS I AG+N +W S SG+FAFGFYRL NNLYL+GIWFD
Sbjct: 9   YLLILAINSCGLHAQILPNISLGSGITAGTNDSWRSLSGEFAFGFYRLQNNLYLMGIWFD 68

Query: 66  KIPQ-TLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQDD 125
           KIP+ TLVWSANRD PA   S+++L   GQ  L++ NG  +Q ++    A AS G MQ+D
Sbjct: 69  KIPERTLVWSANRDTPAEAGSIIRLTFAGQLFLTYSNG-SVQSVY--SGAAASLGFMQND 128

Query: 126 GNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFS-AKNISDFSTGNFMLQMQKDGN 185
           GNFVL+++N   + QSFDSPTDTLLP QIL   +K++S AK  +D+STGNFML+MQ DGN
Sbjct: 129 GNFVLRNANSRVIWQSFDSPTDTLLPTQILTNGEKLYSNAKGTTDYSTGNFMLEMQFDGN 188

Query: 186 LVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPAPVG 245
           LVLS YHFS+ GYW+T  T + N  LVF+ S+A +Y+ N  N     + +LT N  AP G
Sbjct: 189 LVLSAYHFSDPGYWYT-GTLVSNDSLVFN-SNASLYIVNSTN---DIIYSLTANFSAPAG 248

Query: 246 DYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVTC 305
           +YYHRA I+  G+F+QYVY KSN S WI VW A+ +PC VN VCG+NG+C SPDN+TVTC
Sbjct: 249 EYYHRAMIDDMGNFQQYVYHKSNSSGWISVWKAIHEPCFVNAVCGVNGMCSSPDNETVTC 308

Query: 306 DCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDLVRM 365
           +C+PG++ LDP    KGC PETV+NYC   S +NFT+  I D D       E F+DL R+
Sbjct: 309 NCIPGYIPLDPNHVSKGCHPETVVNYCADPSMRNFTIVAINDTDFPF----EGFADLDRV 368

Query: 366 FNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNS 425
            NVD E CK A+M+DCY +AA+  +S C KKRTPL+N R +  TKG   L++VP+  +N 
Sbjct: 369 LNVDVEGCKVALMDDCYSIAASLVDSRCNKKRTPLLNARKSASTKGITALVKVPMKGRNP 428

Query: 426 SEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASAIGI 485
             + +G+ +N+FN+R  L+I  I++  LAF  GA  ++YHP AR+ IRRK+  S + IGI
Sbjct: 429 G-IQEGEKKNHFNFRAFLQISLIVSATLAFVSGATAIYYHPAARKFIRRKH-SSDTTIGI 488

Query: 486 NFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEFV 545
           NFREF + EL +AT+GF K LG+GSS KV  G L +  + ++IAVK LDK  +++E EF+
Sbjct: 489 NFREFKYSELHEATNGFSKTLGRGSSGKVYSGILRLKNVQIDIAVKKLDKEIDKSEEEFM 548

Query: 546 TELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLFD 605
           TEL+IIGRTYH NLVRLLG+C+EN  Q LLVYELM NG LS  LF+  E  G        
Sbjct: 549 TELKIIGRTYHRNLVRLLGFCVEN-NQRLLVYELMANGTLSNLLFEEEERPG-------- 608

Query: 606 NGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIAD 665
                       W  R E+  GI+RGL YLHEECETQIIHCDVKPQNVLLDANY AKIAD
Sbjct: 609 ------------WDLRAEMVLGISRGLLYLHEECETQIIHCDVKPQNVLLDANYNAKIAD 668

Query: 666 FGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIEL 725
           FG+SKLL KDQTRT+T  RGT GY+APEWLR APVT+KVDVYS+GVMLLEI+CCRRHIEL
Sbjct: 669 FGLSKLLNKDQTRTDTNVRGTMGYLAPEWLRNAPVTSKVDVYSFGVMLLEILCCRRHIEL 728

Query: 726 DRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDAS 785
           +RVEEESEE+DLVLS+WV+S    G LE VV    E+L D +RF+RMAMVG+WCIHP+  
Sbjct: 729 NRVEEESEEDDLVLSDWVVSCIIRGKLEIVVRHDPEVLRDLKRFERMAMVGIWCIHPEPV 777

Query: 786 QRPSMKKVTQMLEGTAEVGTPPLL 808
            RPSMKKVTQMLEGT EVG PP+L
Sbjct: 789 LRPSMKKVTQMLEGTLEVGIPPML 777

BLAST of Bhi01G000601 vs. ExPASy TrEMBL
Match: A0A6M2EQI0 (Receptor-like serine/threonine-protein kinase OS=Populus davidiana OX=266767 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 3.3e-259
Identity = 453/804 (56.34%), Postives = 573/804 (71.27%), Query Frame = 0

Query: 6   FLSFVLLNFYKCLAQNGSPISRGSSIAAGSNHTWLSPSGDFAFGFYRLPNNLYLVGIWFD 65
           FL    L F++  AQ    IS GS+I AGS ++W S S +FAFGFY LPNNLYLVGIWF+
Sbjct: 9   FLLLQFLYFHELHAQIPPNISSGSNITAGSGNSWRSLSDEFAFGFYPLPNNLYLVGIWFN 68

Query: 66  KIPQ-TLVWSANRDNPAPPNSLVKLNRTGQFVLSFPNGIIIQPIFKEQQAPASSGQMQDD 125
           KIP+ TLVWSANRD+PA   S V L   GQ  L+  NG ++Q I+    A A  G MQDD
Sbjct: 69  KIPEGTLVWSANRDSPAVAGSTVILTSGGQLTLTHLNGSVVQ-IY--GGARAQFGFMQDD 128

Query: 126 GNFVLKDSNFVAVGQSFDSPTDTLLPGQILGVDKKMFS-AKNISDFSTGNFMLQMQKDGN 185
           GNFVLKD +   + +SF+SPTDT+LPGQ+L  ++K++S A    D+STGNFMLQMQ DG 
Sbjct: 129 GNFVLKDGSSSVIWESFNSPTDTILPGQVLNGNQKLYSNANGTVDYSTGNFMLQMQPDGK 188

Query: 186 LVLSNYHFSNVGYWFTIATNIENTVLVFDKSSALMYLTNRINPNGQSLRNLTVNVPAPVG 245
           LVLS YHF++ GYW T  T   N  LVF+  +A MYL N     G ++  LT NV  PVG
Sbjct: 189 LVLSAYHFADPGYWLTAETVQNNVHLVFNNDTASMYLANGA---GDNIYALTRNVSTPVG 248

Query: 246 DYYHRATINVHGDFRQYVYPKSNGSEWIKVWGAMRDPCLVNTVCGLNGLCKSPDNDTVTC 305
           DYYHRATIN  GDF+Q+ Y KSN S W +VW A+ +PC+VN +CG+ G+C SP+N+T TC
Sbjct: 249 DYYHRATINDRGDFQQFAYHKSNNSGWTRVWRAINEPCVVNAICGVYGMCSSPNNETATC 308

Query: 306 DCLPGFVHLDPADAWKGCRPETVINYCMGDSGKNFTLQVIEDVDIDLPPESEPFSDLVRM 365
           +C+PG++ LDP    KGC PETV+NYC   S +NFT++VI+D D       E F+DL R+
Sbjct: 309 NCIPGYIPLDPNHLSKGCHPETVVNYCADPSMRNFTIEVIDDADFPF----EGFADLARV 368

Query: 366 FNVDFESCKRAIMEDCYVMAATWKNSTCLKKRTPLMNGRNTNITKGTKTLIRVPLFFKNS 425
            NVD E CK+A+M+DCY ++A+  +S C+KKR PL+N R +  TKG + L++VP+  K++
Sbjct: 369 RNVDLEGCKKALMDDCYSLSASLVDSRCIKKRMPLLNARKSFSTKGRQALVKVPM--KSN 428

Query: 426 SEVHDGKNENNFNYRKVLEIGNIIAGVLAFCFGAVVVFYHPTARRLIRRKNFLSASAIGI 485
            ++ + K  N+F+ R  L+I  I+   LAFCFG   ++YHP  RR I+RK + +AS+IGI
Sbjct: 429 PDIQERKKNNDFDTRGFLKISLIVTATLAFCFGVSAIYYHPAPRRFIKRKRYSNASSIGI 488

Query: 486 NFREFTFQELVDATDGFKKILGQGSSCKVVRGNLHIDGIDVEIAVKVLDKMTERTETEFV 545
           NF+EF + EL  AT+GF K LG+GSS KV  G L +  I ++IAVKVL+K  E+ E EF+
Sbjct: 489 NFQEFKYLELQKATNGFSKTLGRGSSAKVYSGILSMKDIQIDIAVKVLEKSIEKGEKEFM 548

Query: 546 TELRIIGRTYHTNLVRLLGYCIENKKQLLLVYELMPNGALSRFLFDNGENMGALSRFLFD 605
           TEL+IIGRTYH NLVRLLG+C+EN +Q LLVYELM NG+L+  LF  G            
Sbjct: 549 TELKIIGRTYHKNLVRLLGFCVENDQQ-LLVYELMANGSLANLLFGKGS----------- 608

Query: 606 NGENTIKISNCNWTQRVEIAKGIARGLAYLHEECETQIIHCDVKPQNVLLDANYAAKIAD 665
                      NW  R ++   IARGL YLHEECET+IIHCD+KP+NVL+D NY AK+AD
Sbjct: 609 --------ERPNWVLRAKMVLEIARGLLYLHEECETRIIHCDIKPENVLIDNNYTAKLAD 668

Query: 666 FGISKLLKKDQTRTNTEARGTAGYMAPEWLRGAPVTAKVDVYSYGVMLLEIICCRRHIEL 725
           FG+SKLL KDQTRT+T  RGT GY+APEW+R   VT+KVDVYS+GVMLLEI+CCRRHIE 
Sbjct: 669 FGLSKLLNKDQTRTDTNLRGTVGYLAPEWIRNEQVTSKVDVYSFGVMLLEILCCRRHIEP 728

Query: 726 DRVEEESEEEDLVLSNWVLSRAAAGNLETVVGDKAEILMDFERFKRMAMVGLWCIHPDAS 785
            RVEEESEE+DLVLS+WV+S  AAG LETVVG   E+L DF+RF+RM +VGLWCIHP A 
Sbjct: 729 SRVEEESEEDDLVLSDWVISCMAAGKLETVVGHDPEVLSDFKRFERMTLVGLWCIHPHAM 780

Query: 786 QRPSMKKVTQMLEGTAEVGTPPLL 808
            RPSMKKV QMLEGT+E+G PP L
Sbjct: 789 SRPSMKKVIQMLEGTSEIGIPPSL 780

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G60900.11.5e-11033.74receptor-like protein kinase 1 [more]
AT4G00340.18.5e-9031.70receptor-like protein kinase 4 [more]
AT2G19130.19.8e-8630.36S-locus lectin protein kinase family protein [more]
AT1G34300.11.4e-7929.49lectin protein kinase family protein [more]
AT5G35370.11.2e-7027.43S-locus lectin protein kinase family protein [more]
Match NameE-valueIdentityDescription
Q7FAZ33.2e-13436.55G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 OS=Oryza sa... [more]
A0A075F7E91.5e-13135.99G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 OS=Oryza sa... [more]
Q25AG31.3e-13035.25G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 OS=Oryza sa... [more]
Q0JEU62.2e-13035.34G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 OS=Oryza sa... [more]
Q25AG22.8e-13036.73G-type lectin S-receptor-like serine/threonine-protein kinase LECRK4 OS=Oryza sa... [more]
Match NameE-valueIdentityDescription
XP_038896440.10.0e+00100.00G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 [Benincasa ... [more]
XP_022955921.10.0e+0072.33G-type lectin S-receptor-like serine/threonine-protein kinase LECRK4 [Cucurbita ... [more]
KAG7018695.10.0e+0072.33G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 [Cucurbita ... [more]
XP_023526919.10.0e+0069.23G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 [Cucurbita ... [more]
XP_021665706.18.7e-26258.46G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 [Hevea bras... [more]
Match NameE-valueIdentityDescription
A0A6J1GV650.0e+0072.33Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A5B7C1T67.7e-26458.55Receptor-like serine/threonine-protein kinase OS=Davidia involucrata OX=16924 GN... [more]
A0A067KVC53.9e-26057.50Receptor-like serine/threonine-protein kinase OS=Jatropha curcas OX=180498 GN=JC... [more]
A0A2C9W8L12.0e-25957.71Receptor-like serine/threonine-protein kinase OS=Manihot esculenta OX=3983 GN=MA... [more]
A0A6M2EQI03.3e-25956.34Receptor-like serine/threonine-protein kinase OS=Populus davidiana OX=266767 PE=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001480Bulb-type lectin domainSMARTSM00108blect_4coord: 30..144
e-value: 1.0E-16
score: 71.5
IPR001480Bulb-type lectin domainPFAMPF01453B_lectincoord: 70..160
e-value: 4.3E-14
score: 52.8
IPR001480Bulb-type lectin domainPROSITEPS50927BULB_LECTINcoord: 16..142
score: 11.097985
IPR001480Bulb-type lectin domainCDDcd00028B_lectincoord: 34..144
e-value: 7.58449E-20
score: 83.9007
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 498..795
e-value: 2.5E-21
score: 86.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 498..807
score: 33.983097
IPR036426Bulb-type lectin domain superfamilyGENE3D2.90.10.10coord: 145..268
e-value: 1.3E-8
score: 36.8
IPR036426Bulb-type lectin domain superfamilyGENE3D2.90.10.10coord: 18..144
e-value: 1.2E-10
score: 43.5
IPR036426Bulb-type lectin domain superfamilySUPERFAMILY51110alpha-D-mannose-specific plant lectinscoord: 60..190
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 480..577
e-value: 5.5E-21
score: 76.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 592..802
e-value: 3.4E-56
score: 192.0
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 523..717
e-value: 1.1E-10
score: 38.3
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 504..721
e-value: 8.4E-21
score: 72.5
NoneNo IPR availablePANTHERPTHR47976G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5coord: 5..807
NoneNo IPR availablePANTHERPTHR47976:SF64SERINE/THREONINE-PROTEIN KINASEcoord: 5..807
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 500..794
e-value: 3.1E-45
score: 154.5
IPR024171S-receptor-like serine/threonine-protein kinasePIRSFPIRSF000641SRKcoord: 1..808
e-value: 1.3E-179
score: 597.1
IPR000858S-locus glycoprotein domainPFAMPF00954S_locus_glycopcoord: 244..310
e-value: 1.9E-8
score: 34.8
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 641..653
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 470..795

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000601Bhi01M000601mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0048544 recognition of pollen
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity