Bhi01G000598 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000598
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionthermospermine synthase ACAULIS5-like
Locationchr1: 15544078 .. 15547229 (+)
RNA-Seq ExpressionBhi01G000598
SyntenyBhi01G000598
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGAAACCACGCGACCAAATCATCCTTACAAAATCTCAAACGTTGGTTCTATGAAACATTACGAAAATCCAAAACGATTTTTTTCCCCTTATTTTTTGAAGCATACTTTACAACAACGACTACAGGCAAGCCATTTTCCAATGTCATTAATTTTCTCAACTCCCTCTCTCTGTTCTCTTTTTCTAATTTCCAAATCAAAATCATACTTCTCTTCACATTTTCCCTTGCTTCCTTCTTCCTTAAATACCCCTTCTTCCCTCCTTTCTTCTCCATCAAATCTTTCTATCCTTCTCTGTTTCTTTTTCTTTCCTCTCAGTTTCTTCTCTCTGCACCAACAATGGCTGATGTTTCCTCTGCTAATTTGGTTAACAATGGTAATGAACTTATTGAGAAGGGCCATTCCTTAAATGGCTATCGAAAGAGCTGTTGGTATGAGGAAGAAATTGAAGAGAATTTAAGATGGTCCTTTGCTCTCAATAGGTACTTGTTTTCTTACATTTTATATCAAAGTTTTTTAACCGTATTTCGTTTTTAGTTTTCTGTTTTTGATATTTATGTTTGTTTACTTTCAATTTCTCTGTTAATAGAAAGTGAATAACAAAACATATATAAACTTATTTGGGTAGTACTAAAAATGAAAAACCAAATAGTTATCGAATAAACCTTATATTTTGGATTATTTTAAGTGGAGTTCTCGAGACAAACTCATTAAACTATACTCTCAATGTTCTAAGCTGTCTGTTGGATCTTTTACTGTGGTTGTTTTGCTCTAGGCTTAATGAAGGTCCATCAAGTTGAAATAGGGTTGAGATCTTTCAAAAAGTAACACACTTTTTCTCTCCCACTTCAAATCAAACTCAATAAAATAATGGTTTGGTCTTGGAAAAGCCAGACAAGAAACCTTAAAACACAAAAAAGTAGTTTTAGTTTAGGGATTGTTCTTCACTACTTGTCACACAAAAATCTACCTACTTTGTAATGAACTTTTTATTTGGTAATCTTGTAGCATACTTCATACTGGAGCTTCTCAGTATCAAGACATCGCGTTACTCGACACGAAGCCGTTCGGGAAAGCGCTGGTCATCGATGGAAAGCTTCAAAGTGCAGAAACTGATGAATTCATATACCATGAATGCCTTGTTCACCCTGCACTTCTCCATCATCCCAAGTACTTACAGTTTCTAATAGTTTAATTCTCTCTAAAGAATCTCAAGTATAAAATTCTAATATCTTCCTATATTGTGATGTTTTTAACCCAGTCCAAAGACCATCTTTATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAGCTACTTAGACACAAGACTGTGGAGAAAGTTGTAATGTGCGACATCGATGAGGTTAAATTTCTTTTTGTTTCGTTTTCTTCTTTTTTAAGCCATTAATCAATACAAAAAAAGCTCTCTAAAACAATAATGATGGATTATTAACCAAATGAACAGGAAGTTGTCGATTTCTGCAAGTCATACTTGATAGTAAACAAAGAAGCTTTCTGTGATCCAAGGCTTGAACTTATCATTAACGATGCCAGGTTCGATTTCAATTATTGCATTTTACGATTCTTCTTACGTTATAGTTGCTATAAGTTCTGGTGGGTTTATGTTGCAGAGCTGAGCTTGAGAACCGAACTGAGCTATACGACGTGATTGTGGGTGATCTTGCAGACCCAATTGAGGGTGGTCCTTGTTATAAACTCTATACTAAATCTTTCTATGAGTATACTGTGAAGCCTAGATTGAAACAAGATGGTATATTTATCACTCAGGTATGTGTGAGATATGAAAATGAAAGAAGTTTGTGAATATATATTAGATTAGGAACTCAACTTGTTAATATCTGTGCAGGCTGGTCCAGCTGGAATTTTCAGCCATACTGAAGTGTTTTCTTGCATCTACAATACTCTAAAGCAAGTTTTTAAATGTGAGAGCAAAACTTGAGGCTATATTATTGTTGTTGGTTTTAGGCCTAAGCTTTTTTAAGTGATTTTTTTCCTTTTTTTCTTTCTAATCTGCAGATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGTAAGAAAAATCATACTTTTTTTTTCATGCTTTTGGGCTGAAAATCAAGGCCAAATCTTTCAATATTGCAAAAGATGAGATGAAAGATATTCTAAAGGCAATTTGGGTCATTTTAAACCAAATAATTTTTCAAGGTTGCATTCAAAACTAGTCGCGCTTGTGTACGCTTAGAACACTATTCATTACTGAGAAATGCTTGGTTCGTCCATGAGAATCAAAGTAGGGAAAACAATGATCCGATGACGAGTATTTCATTAAAACGAAACAATATCAGTTCCAAACTGATAAACTTTGAAATGGGGCTCTAGTTTTCTTTTTCTATCATTTCTTTGGAATGTGTTTTAAGCAAGATTTTTTTCTTGAAACTTGCAGGCCTCAGACACCCCTTTTGTTTTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCACAGGGGAAAACAAGTACTTCGATGGAAAAACATTCTCATCTGCCTCTATATTAAGCAAAGCAGTTCGGAAGTCGTAAGTACACATTTTTGTTTTGTTTTCCTTCTGATTTATTTCAAGATTTGGGAGATGTTAAGATTGGCAAGAAACTTTGCAGACTCGACAACGAGACGCACGTGTACACGGAAGGAACAGCGAGGTTCATATATGGATATGGAGCTGCAGCCTGCAAGCTCAACCAAGTTTGATAGCTTTTCATAAGAGAGGAAAAGAGAAGAAGAAAGAAAGGGCCCCTAAATAAGAGAATATAGAAATCCATCATGTCAGCCTAGAAATGAAACAAAATCTCCTCTAGGCTTCCTTTTTTTTTTTTTCCCTCCCCTTTTTTTGTGTATTGTTTTTTAGTTTTGCTTTCTTATTGTTTATGTAGTGACTTTAAAGTTACAAATATAATAGTAATGAAAAGAGGGGCTTTTAGTTTATATGTTTCTTGTAGTGATGATATTTTATATCCCACTCTCAACACTCTTCCTTGGACAAGGCCCAACTAGCAAAATTGTGGGACACTTTTATTGATTGATATGGAGATGAAATTTAAGCCTTCGATATGAAATGAATTTTT

mRNA sequence

ATTGAAACCACGCGACCAAATCATCCTTACAAAATCTCAAACGTTGGTTCTATGAAACATTACGAAAATCCAAAACGATTTTTTTCCCCTTATTTTTTGAAGCATACTTTACAACAACGACTACAGGCAAGCCATTTTCCAATGTCATTAATTTTCTCAACTCCCTCTCTCTGTTCTCTTTTTCTAATTTCCAAATCAAAATCATACTTCTCTTCACATTTTCCCTTGCTTCCTTCTTCCTTAAATACCCCTTCTTCCCTCCTTTCTTCTCCATCAAATCTTTCTATCCTTCTCTGTTTCTTTTTCTTTCCTCTCAGTTTCTTCTCTCTGCACCAACAATGGCTGATGTTTCCTCTGCTAATTTGGTTAACAATGGTAATGAACTTATTGAGAAGGGCCATTCCTTAAATGGCTATCGAAAGAGCTGTTGGTATGAGGAAGAAATTGAAGAGAATTTAAGATGGTCCTTTGCTCTCAATAGCATACTTCATACTGGAGCTTCTCAGTATCAAGACATCGCGTTACTCGACACGAAGCCGTTCGGGAAAGCGCTGGTCATCGATGGAAAGCTTCAAAGTGCAGAAACTGATGAATTCATATACCATGAATGCCTTGTTCACCCTGCACTTCTCCATCATCCCAATCCAAAGACCATCTTTATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAGCTACTTAGACACAAGACTGTGGAGAAAGTTGTAATGTGCGACATCGATGAGGAAGTTGTCGATTTCTGCAAGTCATACTTGATAGTAAACAAAGAAGCTTTCTGTGATCCAAGGCTTGAACTTATCATTAACGATGCCAGAGCTGAGCTTGAGAACCGAACTGAGCTATACGACGTGATTGTGGGTGATCTTGCAGACCCAATTGAGGGTGGTCCTTGTTATAAACTCTATACTAAATCTTTCTATGAGTATACTGTGAAGCCTAGATTGAAACAAGATGGTATATTTATCACTCAGGCTGGTCCAGCTGGAATTTTCAGCCATACTGAAGTGTTTTCTTGCATCTACAATACTCTAAAGCAAGTTTTTAAATATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGCCTCAGACACCCCTTTTGTTTTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCACAGGGGAAAACAAGTACTTCGATGGAAAAACATTCTCATCTGCCTCTATATTAAGCAAAGCAGTTCGGAAGTCACTCGACAACGAGACGCACGTGTACACGGAAGGAACAGCGAGGTTCATATATGGATATGGAGCTGCAGCCTGCAAGCTCAACCAAGTTTGATAGCTTTTCATAAGAGAGGAAAAGAGAAGAAGAAAGAAAGGGCCCCTAAATAAGAGAATATAGAAATCCATCATGTCAGCCTAGAAATGAAACAAAATCTCCTCTAGGCTTCCTTTTTTTTTTTTTCCCTCCCCTTTTTTTGTGTATTGTTTTTTAGTTTTGCTTTCTTATTGTTTATGTAGTGACTTTAAAGTTACAAATATAATAGTAATGAAAAGAGGGGCTTTTAGTTTATATGTTTCTTGTAGTGATGATATTTTATATCCCACTCTCAACACTCTTCCTTGGACAAGGCCCAACTAGCAAAATTGTGGGACACTTTTATTGATTGATATGGAGATGAAATTTAAGCCTTCGATATGAAATGAATTTTT

Coding sequence (CDS)

ATGGCTGATGTTTCCTCTGCTAATTTGGTTAACAATGGTAATGAACTTATTGAGAAGGGCCATTCCTTAAATGGCTATCGAAAGAGCTGTTGGTATGAGGAAGAAATTGAAGAGAATTTAAGATGGTCCTTTGCTCTCAATAGCATACTTCATACTGGAGCTTCTCAGTATCAAGACATCGCGTTACTCGACACGAAGCCGTTCGGGAAAGCGCTGGTCATCGATGGAAAGCTTCAAAGTGCAGAAACTGATGAATTCATATACCATGAATGCCTTGTTCACCCTGCACTTCTCCATCATCCCAATCCAAAGACCATCTTTATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAGCTACTTAGACACAAGACTGTGGAGAAAGTTGTAATGTGCGACATCGATGAGGAAGTTGTCGATTTCTGCAAGTCATACTTGATAGTAAACAAAGAAGCTTTCTGTGATCCAAGGCTTGAACTTATCATTAACGATGCCAGAGCTGAGCTTGAGAACCGAACTGAGCTATACGACGTGATTGTGGGTGATCTTGCAGACCCAATTGAGGGTGGTCCTTGTTATAAACTCTATACTAAATCTTTCTATGAGTATACTGTGAAGCCTAGATTGAAACAAGATGGTATATTTATCACTCAGGCTGGTCCAGCTGGAATTTTCAGCCATACTGAAGTGTTTTCTTGCATCTACAATACTCTAAAGCAAGTTTTTAAATATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGCCTCAGACACCCCTTTTGTTTTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCACAGGGGAAAACAAGTACTTCGATGGAAAAACATTCTCATCTGCCTCTATATTAAGCAAAGCAGTTCGGAAGTCACTCGACAACGAGACGCACGTGTACACGGAAGGAACAGCGAGGTTCATATATGGATATGGAGCTGCAGCCTGCAAGCTCAACCAAGTTTGA

Protein sequence

MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSASILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV
Homology
BLAST of Bhi01G000598 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 462.2 bits (1188), Expect = 3.5e-130
Identity = 211/328 (64.33%), Postives = 266/328 (81.10%), Query Frame = 0

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 8   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 67

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPKT+FIMGGGEGS ARE+L+H
Sbjct: 68  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 127

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LEL+I DA+AELE R E +D+IVGDL
Sbjct: 128 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 187

Query: 189 ADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 188 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 247

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSASILS 308
           YV  Y+AH+PSFADTWGWVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+ L+
Sbjct: 248 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 307

Query: 309 KAVRKSLDNETHVYTEGTARFIYGYGAA 332
           K +  +L+ ET VY+E  ARFI+G+G A
Sbjct: 308 KTISLALEKETEVYSEENARFIHGHGVA 335

BLAST of Bhi01G000598 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 462.2 bits (1188), Expect = 3.5e-130
Identity = 211/328 (64.33%), Postives = 266/328 (81.10%), Query Frame = 0

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 1   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 60

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPKT+FIMGGGEGS ARE+L+H
Sbjct: 61  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 120

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LEL+I DA+AELE R E +D+IVGDL
Sbjct: 121 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 180

Query: 189 ADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 181 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 240

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSASILS 308
           YV  Y+AH+PSFADTWGWVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+ L+
Sbjct: 241 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 300

Query: 309 KAVRKSLDNETHVYTEGTARFIYGYGAA 332
           K +  +L+ ET VY+E  ARFI+G+G A
Sbjct: 301 KTISLALEKETEVYSEENARFIHGHGVA 328

BLAST of Bhi01G000598 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 156.8 bits (395), Expect = 3.2e-38
Identity = 87/280 (31.07%), Postives = 153/280 (54.64%), Query Frame = 0

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELII 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ L+I
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELENRTE-LYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F++ +V   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVMAS----DTPFVLNADD 282
             ++ H ++   I +  +++FK  V Y+   +P++ +   G+++ S    D  F    + 
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNP 344

Query: 283 LDQRIKQRITGENKYFDGKTFSSASILSKAVRKSLDNETH 316
           +D+    +  G  K+++ +  S+A  L    +K ++++ +
Sbjct: 345 IDES-SSKSNGPLKFYNAEIHSAAFCLPSFAKKVIESKAN 378

BLAST of Bhi01G000598 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 156.0 bits (393), Expect = 5.4e-38
Identity = 80/225 (35.56%), Postives = 128/225 (56.89%), Query Frame = 0

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELII 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ L+I
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELENRTE-LYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F++ +V   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSFADTWG-WVMA 265
             ++ H ++   I +  +++FK  V Y+   +P++   WG W+ A
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYA 324

BLAST of Bhi01G000598 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 154.1 bits (388), Expect = 2.0e-37
Identity = 93/285 (32.63%), Postives = 153/285 (53.68%), Query Frame = 0

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  IL  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L    N
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELII 162
           PK + ++GGG+G   RE+ RH +VE++ +C+ID+ VVD  K Y       + DPR+ LII
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 163 NDARAELENRTE-LYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F+E +V   L+  G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFE-SVNRALRPGGVVCTQA-- 244

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVM-ASDTPFVLNADDLDQ 282
             ++ H ++   I +  + +FK  V Y+   +P++ +   G+++ +S+ P V    D  +
Sbjct: 245 ESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQV----DFKK 304

Query: 283 RIKQRITGEN--------KYFDGKTFSSASILSKAVRKSLDNETH 316
            +    T E+        KY++ +  S+A  L    +K +D++ +
Sbjct: 305 PVSLIDTDESSIKSHCPLKYYNAEIHSAAFCLPSFAKKVIDSKAN 340

BLAST of Bhi01G000598 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 4.9e-129
Identity = 211/328 (64.33%), Postives = 266/328 (81.10%), Query Frame = 0

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 8   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 67

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPKT+FIMGGGEGS ARE+L+H
Sbjct: 68  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 127

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LEL+I DA+AELE R E +D+IVGDL
Sbjct: 128 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 187

Query: 189 ADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 188 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 247

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSASILS 308
           YV  Y+AH+PSFADTWGWVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+ L+
Sbjct: 248 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 307

Query: 309 KAVRKSLDNETHVYTEGTARFIYGYGAA 332
           K +  +L+ ET VY+E  ARFI+G+G A
Sbjct: 308 KTISLALEKETEVYSEENARFIHGHGVA 335

BLAST of Bhi01G000598 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.3e-57
Identity = 118/277 (42.60%), Postives = 170/277 (61.37%), Query Frame = 0

Query: 45  ALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK 104
           A+  ++    + YQ   L++   FGKAL++DGK+QS  TDEFIYHE LVHP LL   NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 105 TIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIIN 164
            I I+GGGEG+T RE+L+HKT++ V M DID  V+DF K YL   ++ AF +P+ +L+I 
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 165 DARAELENRTELYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 224
           D    ++   E +D +V DL DPI+  P   LYTK FYE     R+ + GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 225 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASD--TPFVLNADDLDQRIK 284
            FS  E FS IYNT+K VFK V     ++P+F   WG+V ASD   P   + ++++ RIK
Sbjct: 200 -FS-LETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIK 259

Query: 285 QRITGENKYFDGKTFSSASILSKAVRKSLDNETHVYT 319
           +RI G  +++DG+T  +   + K +R+ L+ E  + T
Sbjct: 260 ERIDGSLRFYDGETHITMFSIPKHIREVLEKENKIST 291

BLAST of Bhi01G000598 vs. ExPASy Swiss-Prot
Match: A2BJU2 (Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5) OX=415426 GN=speE2 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 9.5e-56
Identity = 120/272 (44.12%), Postives = 167/272 (61.40%), Query Frame = 0

Query: 49  ILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFI 108
           I   GA++YQ+  + +    GKALV+DGK+QS+  DE  YHE LVHP LL H  P+ + +
Sbjct: 27  IYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQCPRKVLV 86

Query: 109 MGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIINDARA 168
           +GGGEG+T RE+LRH  VE V M DIDEE+V+  K +L   ++ AF   +LEL+I D R 
Sbjct: 87  IGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRR 146

Query: 169 ELENRTELYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSH 228
            +EN    YD I+ DL DP+EGGP  +LYT  FY   VK  L+  G  +TQA    +   
Sbjct: 147 YVENCHRKYDAIILDLVDPMEGGPAARLYTLEFYR-AVKGCLRPGGAVVTQATSPTL--S 206

Query: 229 TEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDT--PFVLNADDLDQRIKQRIT 288
             V++ I NTL +VF  V PY +++ S+   WG+V ASDT  P  L+A ++D+ I  RI 
Sbjct: 207 PRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAASDTVDPAKLSAKEVDELIAARIR 266

Query: 289 GENKYFDGKTFSSASILSKAVRKSLDNETHVY 318
           G+ +++DG+T      L   VR+ L +ET  Y
Sbjct: 267 GQLRFYDGETHEWMFRLPLPVRQVL-SETRDY 294

BLAST of Bhi01G000598 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.3e-54
Identity = 115/290 (39.66%), Postives = 173/290 (59.66%), Query Frame = 0

Query: 46  LNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKT 105
           +  ++ +G + +QD  L ++K FGK L++D  +QS E DE+IYHE LVHPA+L HP PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 106 IFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIIND 165
           + I+GGGEG+T RE+L+H TVEK VM DID E+V+  K ++   ++ AF DPR  L+I+D
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 166 ARAELENRTELYDVIVGDLADPI-EGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 225
           ARA LE   E YDV++ DL DP+ E  P   LYT  FY   VK  L   G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMI- 200

Query: 226 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDT--PFVLNADDLDQRIK 285
           + +H  V   ++ T+++ F+YV  Y  HIP F   +G+++ASD   P   +   ++ RI+
Sbjct: 201 LLTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIR 260

Query: 286 QRITGENKYFDGKTFSSASILSKAVRKSLDNETHVYTEGTARFIYGYGAA 332
           +R     ++       +  +L K + ++L+ ET V T+    ++   G A
Sbjct: 261 ERNLA-LRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEGEA 307

BLAST of Bhi01G000598 vs. ExPASy Swiss-Prot
Match: Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.3e-54
Identity = 115/290 (39.66%), Postives = 173/290 (59.66%), Query Frame = 0

Query: 46  LNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKT 105
           +  ++ +G + +QD  L ++K FGK L++D  +QS E DE+IYHE LVHPA+L HP PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 106 IFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIIND 165
           + I+GGGEG+T RE+L+H TVEK VM DID E+V+  K ++   ++ AF DPR  L+I+D
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 166 ARAELENRTELYDVIVGDLADPI-EGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 225
           ARA LE   E YDV++ DL DP+ E  P   LYT  FY   VK  L   G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMI- 200

Query: 226 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDT--PFVLNADDLDQRIK 285
           + +H  V   ++ T+++ F+YV  Y  HIP F   +G+++ASD   P   +   ++ RI+
Sbjct: 201 LLTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIR 260

Query: 286 QRITGENKYFDGKTFSSASILSKAVRKSLDNETHVYTEGTARFIYGYGAA 332
           +R     ++       +  +L K + ++L+ ET V T+    ++   G A
Sbjct: 261 ERNLA-LRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEGEA 307

BLAST of Bhi01G000598 vs. NCBI nr
Match: XP_038880186.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 703.4 bits (1814), Expect = 9.7e-199
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
           ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV
Sbjct: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 338

BLAST of Bhi01G000598 vs. NCBI nr
Match: XP_004134447.1 (thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN56994.1 hypothetical protein Csa_011523 [Cucumis sativus])

HSP 1 Score: 689.1 bits (1777), Expect = 1.9e-194
Identity = 332/338 (98.22%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
            LSKAVRKSLDNET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

BLAST of Bhi01G000598 vs. NCBI nr
Match: XP_008438686.1 (PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0049369.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa] >TYK17190.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa])

HSP 1 Score: 688.0 bits (1774), Expect = 4.2e-194
Identity = 331/338 (97.93%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHALNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
            LSKAVRKSLDNET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

BLAST of Bhi01G000598 vs. NCBI nr
Match: XP_022138299.1 (thermospermine synthase ACAULIS5-like [Momordica charantia])

HSP 1 Score: 686.4 bits (1770), Expect = 1.2e-193
Identity = 329/338 (97.34%), Postives = 332/338 (98.22%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLEL+INDARAELE+R E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMAS+TPFVLNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASNTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
            LSKAVRKSLD ETHVYTEGTARFIYGYGAAACKLNQV
Sbjct: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 338

BLAST of Bhi01G000598 vs. NCBI nr
Match: KAG6597450.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 686.0 bits (1769), Expect = 1.6e-193
Identity = 332/339 (97.94%), Postives = 334/339 (98.53%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENRTE YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYG-AAACKLNQV 339
            LSKAVRKSLDNETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

BLAST of Bhi01G000598 vs. ExPASy TrEMBL
Match: A0A0A0L7M9 (Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 9.2e-195
Identity = 332/338 (98.22%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
            LSKAVRKSLDNET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

BLAST of Bhi01G000598 vs. ExPASy TrEMBL
Match: A0A5D3D0P3 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00350 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.0e-194
Identity = 331/338 (97.93%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHALNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
            LSKAVRKSLDNET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

BLAST of Bhi01G000598 vs. ExPASy TrEMBL
Match: A0A1S3AX06 (thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103483717 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.0e-194
Identity = 331/338 (97.93%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHALNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRITGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
            LSKAVRKSLDNET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

BLAST of Bhi01G000598 vs. ExPASy TrEMBL
Match: A0A6J1C9S2 (thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC111009509 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 6.0e-194
Identity = 329/338 (97.34%), Postives = 332/338 (98.22%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLEL+INDARAELE+R E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMAS+TPFVLNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASNTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
            LSKAVRKSLD ETHVYTEGTARFIYGYGAAACKLNQV
Sbjct: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 338

BLAST of Bhi01G000598 vs. ExPASy TrEMBL
Match: A0A6J1F7M2 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441602 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.0e-193
Identity = 331/339 (97.64%), Postives = 334/339 (98.53%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENRTE YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYE+TVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEHTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYG-AAACKLNQV 339
            LSKAVRKSLDNETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G19530.13.5e-13064.33S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G19530.23.5e-13064.33S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23820.13.2e-3831.07spermidine synthase 1 [more]
AT1G23820.25.4e-3835.56spermidine synthase 1 [more]
AT1G70310.12.0e-3732.63spermidine synthase 2 [more]
Match NameE-valueIdentityDescription
Q9S7X64.9e-12964.33Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV... [more]
Q975S51.3e-5742.60Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ... [more]
A2BJU29.5e-5644.12Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / ... [more]
Q72K552.3e-5439.66Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ... [more]
Q5SK282.3e-5439.66Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS... [more]
Match NameE-valueIdentityDescription
XP_038880186.19.7e-199100.00thermospermine synthase ACAULIS5-like [Benincasa hispida][more]
XP_004134447.11.9e-19498.22thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN56994.1 hypothetical prot... [more]
XP_008438686.14.2e-19497.93PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0049369.1 th... [more]
XP_022138299.11.2e-19397.34thermospermine synthase ACAULIS5-like [Momordica charantia][more]
KAG6597450.11.6e-19397.94Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
A0A0A0L7M99.2e-19598.22Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1[more]
A0A5D3D0P32.0e-19497.93Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3AX062.0e-19497.93thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103483717 PE... [more]
A0A6J1C9S26.0e-19497.34thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A6J1F7M23.0e-19397.64thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 31..82
e-value: 2.5E-13
score: 49.7
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 85..262
e-value: 2.4E-41
score: 141.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 82..334
e-value: 8.5E-77
score: 260.0
NoneNo IPR availablePANTHERPTHR43317:SF9SPERMINE SYNTHASEcoord: 1..331
NoneNo IPR availablePANTHERPTHR43317THERMOSPERMINE SYNTHASE ACAULIS5coord: 1..331
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 110..218
e-value: 2.09959E-5
score: 41.2615
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 29..81
e-value: 7.1E-15
score: 56.8
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 30..311
score: 31.202271
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 29..266
score: 96.741638
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 29..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000598Bhi01M000598mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006596 polyamine biosynthetic process
molecular_function GO:0010487 thermospermine synthase activity
molecular_function GO:0003824 catalytic activity