Bhi01G000446 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G000446
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	L-gulonolactone oxidase
Location	chr1: 11098415 .. 11098933 (+)
RNA-Seq Expression	Bhi01G000446
Synteny	Bhi01G000446

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTAGGTGTTGGTGGTGGTCGTACAATGTTGCCTTGGTTTTCTTGTTGGTCGTTATTCTGTCTGACTGTAGTCCCCCGGAGGATCCGATCAAATGCGGATCCGTATCCCCAAATAAACACGCCAATTGCACCATCACAAATGCCTACGCCGCGTTTCCAGACCGAACCATCTGCCGTGCAGCGGAGGTGATATACCCAACAACAGAGGCGGAGGTGATGTTGGCGGTGGCGGCAGCGACGAGAGGTATGAGGAAAATGAAGGTGGGGACTCGGTTCTCTCACAGCATTCCGAAGCTGTCGTGTCCGGAGGGGGAAGATGGGGTGGTGATAAGCACCAAGTACCTGAACCGGACTCTGATGGTGGACGTGGAGGGAATGAGCATAAGCGTGGAGGGGGGAGCCACGGTGAGGCAGATCATAGAGGAGGTCTGCCGCAGCTGGGCTGGCGCTGCCGCATACGCCTTACTGGTGGGGGCTCACCATAGGCGGCCTCCTAAGCACCGGGGCACATGGTAG

mRNA sequence

ATGCGTAGGTGTTGGTGGTGGTCGTACAATGTTGCCTTGGTTTTCTTGTTGGTCGTTATTCTGTCTGACTGTAGTCCCCCGGAGGATCCGATCAAATGCGGATCCGTATCCCCAAATAAACACGCCAATTGCACCATCACAAATGCCTACGCCGCGTTTCCAGACCGAACCATCTGCCGTGCAGCGGAGGTGATATACCCAACAACAGAGGCGGAGGTGATGTTGGCGGTGGCGGCAGCGACGAGAGGTATGAGGAAAATGAAGGTGGGGACTCGGTTCTCTCACAGCATTCCGAAGCTGTCGTGTCCGGAGGGGGAAGATGGGGTGGTGATAAGCACCAAGTACCTGAACCGGACTCTGATGGTGGACGTGGAGGGAATGAGCATAAGCGTGGAGGGGGGAGCCACGGTGAGGCAGATCATAGAGGAGGTCTGCCGCAGCTGGGCTGGCGCTGCCGCATACGCCTTACTGGTGGGGGCTCACCATAGGCGGCCTCCTAAGCACCGGGGCACATGGTAG

Coding sequence (CDS)

ATGCGTAGGTGTTGGTGGTGGTCGTACAATGTTGCCTTGGTTTTCTTGTTGGTCGTTATTCTGTCTGACTGTAGTCCCCCGGAGGATCCGATCAAATGCGGATCCGTATCCCCAAATAAACACGCCAATTGCACCATCACAAATGCCTACGCCGCGTTTCCAGACCGAACCATCTGCCGTGCAGCGGAGGTGATATACCCAACAACAGAGGCGGAGGTGATGTTGGCGGTGGCGGCAGCGACGAGAGGTATGAGGAAAATGAAGGTGGGGACTCGGTTCTCTCACAGCATTCCGAAGCTGTCGTGTCCGGAGGGGGAAGATGGGGTGGTGATAAGCACCAAGTACCTGAACCGGACTCTGATGGTGGACGTGGAGGGAATGAGCATAAGCGTGGAGGGGGGAGCCACGGTGAGGCAGATCATAGAGGAGGTCTGCCGCAGCTGGGCTGGCGCTGCCGCATACGCCTTACTGGTGGGGGCTCACCATAGGCGGCCTCCTAAGCACCGGGGCACATGGTAG

Protein sequence

MRRCWWWSYNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGMSISVEGGATVRQIIEEVCRSWAGAAAYALLVGAHHRRPPKHRGTW

Homology

[Close]

BLAST of Bhi01G000446 vs. TAIR 10
Match: AT2G46760.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 147.5 bits (371), Expect = 9.8e-36
Identity = 74/146 (50.68%), Postives = 101/146 (69.18%), Query Frame = 0


Query: 1   MRRCWWWSYNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICR 60
           +   +W +  V      +V  +  +PPEDP+KC  VS N   NCT+TN+Y AFPDR+ CR
Sbjct: 12  LNAAFWRTIFVVHCISTLVFTTISTPPEDPVKC--VSGN--TNCTVTNSYGAFPDRSTCR 71

Query: 61  AAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTL 120
           AA V YPTTEAE++  VAAAT+  RKM+V TR+SHSI KL+C +G DG++ISTK+LN T+
Sbjct: 72  AANVAYPTTEAELISVVAAATKAGRKMRVTTRYSHSITKLACTDGTDGLLISTKFLNHTV 131

Query: 121 MVDVEGMSISVEGGATVRQIIEEVCR 147
             D   M+++VE G T+RQ+I E  +
Sbjct: 132 RTDATAMTLTVESGVTLRQLIAEAAK 153

[Close]

BLAST of Bhi01G000446 vs. TAIR 10
Match: AT2G46740.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 146.0 bits (367), Expect = 2.8e-35
Identity = 75/144 (52.08%), Postives = 101/144 (70.14%), Query Frame = 0


Query: 4   CWWWSYNVALVFLLVVILSDCS-PPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAA 63
           C +W   + L  L  ++ +  S PPEDP+KC  VS N   NC +TN+  AFPDR+ CRAA
Sbjct: 10  CSFWRTLLGLYCLFTLVHTVISTPPEDPVKC--VSGN--TNCIVTNSLGAFPDRSTCRAA 69

Query: 64  EVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMV 123
            V YPTTEAE++  VAAAT+  RKM+V TR+SHSIPKL+C +G DG+ ISTK+LN T+  
Sbjct: 70  NVAYPTTEAELVSIVAAATKAGRKMRVTTRYSHSIPKLTCTDGNDGLFISTKFLNHTVQA 129

Query: 124 DVEGMSISVEGGATVRQIIEEVCR 147
           D + M+++VE G T+RQ+I E  +
Sbjct: 130 DAKAMTLTVESGVTLRQLIAEAAK 149

[Close]

BLAST of Bhi01G000446 vs. TAIR 10
Match: AT2G46750.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 138.3 bits (347), Expect = 5.9e-33
Identity = 68/122 (55.74%), Postives = 90/122 (73.77%), Query Frame = 0


Query: 25  SPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYPTTEAEVMLAVAAATRGM 84
           +PP+DP+KC  VS N   NCT+TN+Y AFPDR+ CRAA V YP TEAE++  VAAAT+  
Sbjct: 28  TPPDDPVKC--VSGN--TNCTVTNSYGAFPDRSTCRAANVAYPKTEAELVSVVAAATQAG 87

Query: 85  RKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGMSISVEGGATVRQIIEEV 144
           RKM+V TR+SHSI KL C +G +G+ ISTK+LN T+  D   M+++VE G T+RQ+I E 
Sbjct: 88  RKMRVTTRYSHSITKLVCTDGTEGLFISTKFLNHTVQADATAMTMTVESGMTLRQLIVEA 145

Query: 145 CR 147
            +
Sbjct: 148 AK 145

[Close]

BLAST of Bhi01G000446 vs. TAIR 10
Match: AT5G56490.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 137.9 bits (346), Expect = 7.7e-33
Identity = 68/139 (48.92%), Postives = 97/139 (69.78%), Query Frame = 0


Query: 8   SYNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYP 67
           S+ ++L+F      S  SPP+DP+ C       +  CT+TN+Y AFPDR+IC+AA+V YP
Sbjct: 2   SFWLSLIFCFFTFASS-SPPDDPVNC----EFGNTMCTVTNSYGAFPDRSICKAAKVEYP 61

Query: 68  TTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGM 127
            TEAE++  VAAATR  +KM+V TR+SHS PKL C +G+DG +ISTK+LN  +  + E  
Sbjct: 62  RTEAELVSIVAAATRAGQKMRVVTRYSHSFPKLVCTDGKDGTLISTKFLNHVVTTNPEAK 121

Query: 128 SISVEGGATVRQIIEEVCR 147
           +++VE G T+RQ+IEE  +
Sbjct: 122 TLTVESGVTLRQLIEEAAK 135

[Close]

BLAST of Bhi01G000446 vs. TAIR 10
Match: AT1G32300.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 135.2 bits (339), Expect = 5.0e-32
Identity = 66/133 (49.62%), Postives = 95/133 (71.43%), Query Frame = 0


Query: 11  VALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYPTTE 70
           ++L+F      +  +PP+DP+KC S     +  CT+TN+Y AFPDR+IC AA+V YP TE
Sbjct: 5   LSLIFFCFCTFASSTPPDDPVKCES----GNNMCTVTNSYGAFPDRSICEAAKVEYPKTE 64

Query: 71  AEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGMSIS 130
           AE++  VAAATR  +K++V TR+ HSIPKL C +G+DGV+ISTK+LN  +  + E  +++
Sbjct: 65  AELVSIVAAATRAGQKVRVVTRYVHSIPKLVCTDGKDGVLISTKFLNNVVGTNPEAKTLT 124

Query: 131 VEGGATVRQIIEE 144
           VE G T+RQ+I E
Sbjct: 125 VESGVTLRQLIGE 133

[Close]

BLAST of Bhi01G000446 vs. ExPASy Swiss-Prot
Match: O81032 (Probable L-gulonolactone oxidase 6 OS=Arabidopsis thaliana OX=3702 GN=GULLO6 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.4e-34
Identity = 74/146 (50.68%), Postives = 101/146 (69.18%), Query Frame = 0


Query: 1   MRRCWWWSYNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICR 60
           +   +W +  V      +V  +  +PPEDP+KC  VS N   NCT+TN+Y AFPDR+ CR
Sbjct: 12  LNAAFWRTIFVVHCISTLVFTTISTPPEDPVKC--VSGN--TNCTVTNSYGAFPDRSTCR 71

Query: 61  AAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTL 120
           AA V YPTTEAE++  VAAAT+  RKM+V TR+SHSI KL+C +G DG++ISTK+LN T+
Sbjct: 72  AANVAYPTTEAELISVVAAATKAGRKMRVTTRYSHSITKLACTDGTDGLLISTKFLNHTV 131

Query: 121 MVDVEGMSISVEGGATVRQIIEEVCR 147
             D   M+++VE G T+RQ+I E  +
Sbjct: 132 RTDATAMTLTVESGVTLRQLIAEAAK 153

[Close]

BLAST of Bhi01G000446 vs. ExPASy Swiss-Prot
Match: O81030 (L-gulonolactone oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=GULLO5 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 4.0e-34
Identity = 75/144 (52.08%), Postives = 101/144 (70.14%), Query Frame = 0


Query: 4   CWWWSYNVALVFLLVVILSDCS-PPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAA 63
           C +W   + L  L  ++ +  S PPEDP+KC  VS N   NC +TN+  AFPDR+ CRAA
Sbjct: 10  CSFWRTLLGLYCLFTLVHTVISTPPEDPVKC--VSGN--TNCIVTNSLGAFPDRSTCRAA 69

Query: 64  EVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMV 123
            V YPTTEAE++  VAAAT+  RKM+V TR+SHSIPKL+C +G DG+ ISTK+LN T+  
Sbjct: 70  NVAYPTTEAELVSIVAAATKAGRKMRVTTRYSHSIPKLTCTDGNDGLFISTKFLNHTVQA 129

Query: 124 DVEGMSISVEGGATVRQIIEEVCR 147
           D + M+++VE G T+RQ+I E  +
Sbjct: 130 DAKAMTLTVESGVTLRQLIAEAAK 149

[Close]

BLAST of Bhi01G000446 vs. ExPASy Swiss-Prot
Match: Q6NQ66 (L-gulonolactone oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GULLO2 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 8.3e-32
Identity = 68/122 (55.74%), Postives = 90/122 (73.77%), Query Frame = 0


Query: 25  SPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYPTTEAEVMLAVAAATRGM 84
           +PP+DP+KC  VS N   NCT+TN+Y AFPDR+ CRAA V YP TEAE++  VAAAT+  
Sbjct: 28  TPPDDPVKC--VSGN--TNCTVTNSYGAFPDRSTCRAANVAYPKTEAELVSVVAAATQAG 87

Query: 85  RKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGMSISVEGGATVRQIIEEV 144
           RKM+V TR+SHSI KL C +G +G+ ISTK+LN T+  D   M+++VE G T+RQ+I E 
Sbjct: 88  RKMRVTTRYSHSITKLVCTDGTEGLFISTKFLNHTVQADATAMTMTVESGMTLRQLIVEA 145

Query: 145 CR 147
            +
Sbjct: 148 AK 145

[Close]

BLAST of Bhi01G000446 vs. ExPASy Swiss-Prot
Match: Q9FM82 (Probable L-gulonolactone oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GULLO4 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.1e-31
Identity = 68/139 (48.92%), Postives = 97/139 (69.78%), Query Frame = 0


Query: 8   SYNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYP 67
           S+ ++L+F      S  SPP+DP+ C       +  CT+TN+Y AFPDR+IC+AA+V YP
Sbjct: 2   SFWLSLIFCFFTFASS-SPPDDPVNC----EFGNTMCTVTNSYGAFPDRSICKAAKVEYP 61

Query: 68  TTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGM 127
            TEAE++  VAAATR  +KM+V TR+SHS PKL C +G+DG +ISTK+LN  +  + E  
Sbjct: 62  RTEAELVSIVAAATRAGQKMRVVTRYSHSFPKLVCTDGKDGTLISTKFLNHVVTTNPEAK 121

Query: 128 SISVEGGATVRQIIEEVCR 147
           +++VE G T+RQ+IEE  +
Sbjct: 122 TLTVESGVTLRQLIEEAAK 135

[Close]

BLAST of Bhi01G000446 vs. ExPASy Swiss-Prot
Match: Q9C614 (Probable L-gulonolactone oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GULLO1 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 7.1e-31
Identity = 66/133 (49.62%), Postives = 95/133 (71.43%), Query Frame = 0


Query: 11  VALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYPTTE 70
           ++L+F      +  +PP+DP+KC S     +  CT+TN+Y AFPDR+IC AA+V YP TE
Sbjct: 5   LSLIFFCFCTFASSTPPDDPVKCES----GNNMCTVTNSYGAFPDRSICEAAKVEYPKTE 64

Query: 71  AEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGMSIS 130
           AE++  VAAATR  +K++V TR+ HSIPKL C +G+DGV+ISTK+LN  +  + E  +++
Sbjct: 65  AELVSIVAAATRAGQKVRVVTRYVHSIPKLVCTDGKDGVLISTKFLNNVVGTNPEAKTLT 124

Query: 131 VEGGATVRQIIEE 144
           VE G T+RQ+I E
Sbjct: 125 VESGVTLRQLIGE 133

[Close]

BLAST of Bhi01G000446 vs. NCBI nr
Match: XP_038882597.1 (LOW QUALITY PROTEIN: probable L-gulonolactone oxidase 6 [Benincasa hispida])

HSP 1 Score: 294.3 bits (752), Expect = 6.9e-76
Identity = 151/171 (88.30%), Postives = 152/171 (88.89%), Query Frame = 0


Query: 1   MRRCWWWSYNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICR 60
           MRRCWWWSYNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICR
Sbjct: 1   MRRCWWWSYNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICR 60

Query: 61  AAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTL 120
           AAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTL
Sbjct: 61  AAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTL 120

Query: 121 MVDVEGMSISVEGGATVRQIIEEVCRS---------WAGAAAYALL-VGAH 162
           MVDVEGMSISVEGGATVRQIIEEV  +         W G     LL  GAH
Sbjct: 121 MVDVEGMSISVEGGATVRQIIEEVAAAGLALPHTPYWWGLTIGGLLSTGAH 171

[Close]

BLAST of Bhi01G000446 vs. NCBI nr
Match: XP_008437774.1 (PREDICTED: probable L-gulonolactone oxidase 6 [Cucumis melo])

HSP 1 Score: 247.3 bits (630), Expect = 9.7e-62
Identity = 126/177 (71.19%), Postives = 143/177 (80.79%), Query Frame = 0


Query: 1   MRRCWWWS------YNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFP 60
           MRR WWWS       + + VF+ +V++S+CSPPEDPIKCGSVSPNK+ANCTITN+Y AFP
Sbjct: 1   MRRWWWWSSYCVGICSTSAVFMFLVLVSECSPPEDPIKCGSVSPNKYANCTITNSYGAFP 60

Query: 61  DRTICRAAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTK 120
           DR+ICR AEVIYPTTEAEVMLAVAAATRG+RKMKVGTRFSHSIPKL+CPEGE+GVVISTK
Sbjct: 61  DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK 120

Query: 121 YLNRTLMVDVEGMSISVEGGATVRQIIEEVCRS---------WAGAAAYALL-VGAH 162
           YLNRT+ VDVEGMSISVEGGAT+RQIIE+   +         W G     LL  GAH
Sbjct: 121 YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPHTPYWWGLTIGGLLSTGAH 177

[Close]

BLAST of Bhi01G000446 vs. NCBI nr
Match: KAA0048816.1 (putative L-gulonolactone oxidase 6 [Cucumis melo var. makuwa] >TYK20769.1 putative L-gulonolactone oxidase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 247.3 bits (630), Expect = 9.7e-62
Identity = 126/177 (71.19%), Postives = 143/177 (80.79%), Query Frame = 0


Query: 1   MRRCWWWS------YNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFP 60
           MRR WWWS       + + VF+ +V++S+CSPPEDPIKCGSVSPNK+ANCTITN+Y AFP
Sbjct: 1   MRRWWWWSSYCVGICSTSAVFMFLVLVSECSPPEDPIKCGSVSPNKYANCTITNSYGAFP 60

Query: 61  DRTICRAAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTK 120
           DR+ICR AEVIYPTTEAEVMLAVAAATRG+RKMKVGTRFSHSIPKL+CPEGE+GVVISTK
Sbjct: 61  DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK 120

Query: 121 YLNRTLMVDVEGMSISVEGGATVRQIIEEVCRS---------WAGAAAYALL-VGAH 162
           YLNRT+ VDVEGMSISVEGGAT+RQIIE+   +         W G     LL  GAH
Sbjct: 121 YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPHTPYWWGLTIGGLLSTGAH 177

[Close]

BLAST of Bhi01G000446 vs. NCBI nr
Match: XP_004134280.1 (probable L-gulonolactone oxidase 6 [Cucumis sativus] >KAE8650211.1 hypothetical protein Csa_009956 [Cucumis sativus])

HSP 1 Score: 232.6 bits (592), Expect = 2.5e-57
Identity = 123/176 (69.89%), Postives = 140/176 (79.55%), Query Frame = 0


Query: 1   MRRCWWWSYNV-----ALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPD 60
           MRR  W SY V     ++VF+ +V++S+CSPP DPIKC SVSPNK+ANCTITN Y +FPD
Sbjct: 1   MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPD 60

Query: 61  RTICRAAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKY 120
           R+ICR AEVIYPTTEAEVMLAVAAATRG+RKMKVGTRFSHSIPKL+CPEGE+GVVISTKY
Sbjct: 61  RSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKY 120

Query: 121 LNRTLMVDVEGMSISVEGGATVRQIIEEVCRS---------WAGAAAYALL-VGAH 162
           LNRT+ VDVEGMSISVEGGAT+RQIIE+   +         W G     LL  GAH
Sbjct: 121 LNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAH 176

[Close]

BLAST of Bhi01G000446 vs. NCBI nr
Match: XP_023540226.1 (probable L-gulonolactone oxidase 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 183.7 bits (465), Expect = 1.3e-42
Identity = 96/130 (73.85%), Postives = 107/130 (82.31%), Query Frame = 0


Query: 13  LVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYPTTEAE 72
           L  +L+V +S+ +PPEDPIKC   SPN  ANCTITNAYA FPDR ICR  EV YPTTEAE
Sbjct: 14  LFLVLLVSVSESTPPEDPIKCR--SPN--ANCTITNAYATFPDRAICRVGEVKYPTTEAE 73

Query: 73  VMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGMSISVE 132
           V+ AVA ATR MRK+KVGTRF HSIPKL+CP+G DGVVISTKYLNR L VD EGMSI+VE
Sbjct: 74  VISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVE 133

Query: 133 GGATVRQIIE 143
           GG TVR+IIE
Sbjct: 134 GGVTVREIIE 139

[Close]

BLAST of Bhi01G000446 vs. ExPASy TrEMBL
Match: A0A5A7U3K3 (Putative L-gulonolactone oxidase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00290 PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 4.7e-62
Identity = 126/177 (71.19%), Postives = 143/177 (80.79%), Query Frame = 0


Query: 1   MRRCWWWS------YNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFP 60
           MRR WWWS       + + VF+ +V++S+CSPPEDPIKCGSVSPNK+ANCTITN+Y AFP
Sbjct: 1   MRRWWWWSSYCVGICSTSAVFMFLVLVSECSPPEDPIKCGSVSPNKYANCTITNSYGAFP 60

Query: 61  DRTICRAAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTK 120
           DR+ICR AEVIYPTTEAEVMLAVAAATRG+RKMKVGTRFSHSIPKL+CPEGE+GVVISTK
Sbjct: 61  DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK 120

Query: 121 YLNRTLMVDVEGMSISVEGGATVRQIIEEVCRS---------WAGAAAYALL-VGAH 162
           YLNRT+ VDVEGMSISVEGGAT+RQIIE+   +         W G     LL  GAH
Sbjct: 121 YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPHTPYWWGLTIGGLLSTGAH 177

[Close]

BLAST of Bhi01G000446 vs. ExPASy TrEMBL
Match: A0A1S3AUG3 (L-gulonolactone oxidase OS=Cucumis melo OX=3656 GN=LOC103483108 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 4.7e-62
Identity = 126/177 (71.19%), Postives = 143/177 (80.79%), Query Frame = 0


Query: 1   MRRCWWWS------YNVALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFP 60
           MRR WWWS       + + VF+ +V++S+CSPPEDPIKCGSVSPNK+ANCTITN+Y AFP
Sbjct: 1   MRRWWWWSSYCVGICSTSAVFMFLVLVSECSPPEDPIKCGSVSPNKYANCTITNSYGAFP 60

Query: 61  DRTICRAAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTK 120
           DR+ICR AEVIYPTTEAEVMLAVAAATRG+RKMKVGTRFSHSIPKL+CPEGE+GVVISTK
Sbjct: 61  DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK 120

Query: 121 YLNRTLMVDVEGMSISVEGGATVRQIIEEVCRS---------WAGAAAYALL-VGAH 162
           YLNRT+ VDVEGMSISVEGGAT+RQIIE+   +         W G     LL  GAH
Sbjct: 121 YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPHTPYWWGLTIGGLLSTGAH 177

[Close]

BLAST of Bhi01G000446 vs. ExPASy TrEMBL
Match: A0A0A0L3K4 (L-gulonolactone oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G116800 PE=3 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.2e-57
Identity = 123/176 (69.89%), Postives = 140/176 (79.55%), Query Frame = 0


Query: 1   MRRCWWWSYNV-----ALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPD 60
           MRR  W SY V     ++VF+ +V++S+CSPP DPIKC SVSPNK+ANCTITN Y +FPD
Sbjct: 1   MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPD 60

Query: 61  RTICRAAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKY 120
           R+ICR AEVIYPTTEAEVMLAVAAATRG+RKMKVGTRFSHSIPKL+CPEGE+GVVISTKY
Sbjct: 61  RSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKY 120

Query: 121 LNRTLMVDVEGMSISVEGGATVRQIIEEVCRS---------WAGAAAYALL-VGAH 162
           LNRT+ VDVEGMSISVEGGAT+RQIIE+   +         W G     LL  GAH
Sbjct: 121 LNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAH 176

[Close]

BLAST of Bhi01G000446 vs. ExPASy TrEMBL
Match: A0A6J1IG87 (L-gulonolactone oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474382 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 7.1e-42
Identity = 97/143 (67.83%), Postives = 113/143 (79.02%), Query Frame = 0


Query: 1   MRRCWWWSYNVALVFL-LVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTIC 60
           MR    + +   ++FL L+V +S+ +PPEDPIKC   SPN  ANCTITNAYA FPDR IC
Sbjct: 1   MRSSHAFRFTTLILFLVLLVSVSESTPPEDPIKCR--SPN--ANCTITNAYATFPDRAIC 60

Query: 61  RAAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRT 120
           R  EV YPTTEAE++ AVAAATR MRK+KVGTRF HSIPKL+CP+G DGVVISTK+LN  
Sbjct: 61  RVGEVKYPTTEAEMISAVAAATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKFLNWV 120

Query: 121 LMVDVEGMSISVEGGATVRQIIE 143
           L VD EGMSI+VEGG TVR+IIE
Sbjct: 121 LKVDGEGMSITVEGGVTVREIIE 139

[Close]

BLAST of Bhi01G000446 vs. ExPASy TrEMBL
Match: A0A6J1FUH8 (L-gulonolactone oxidase OS=Cucurbita moschata OX=3662 GN=LOC111448536 PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 9.2e-42
Identity = 94/127 (74.02%), Postives = 104/127 (81.89%), Query Frame = 0


Query: 16  LLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYPTTEAEVML 75
           +L+V +S+ +PPEDPIKC   SPN  ANCTITNAYA FPDR ICR  EV YPTTEAEV+ 
Sbjct: 17  VLLVSVSESTPPEDPIKCR--SPN--ANCTITNAYATFPDRAICRVGEVKYPTTEAEVIS 76

Query: 76  AVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGMSISVEGGA 135
           AVA ATR MRK+KVGTRF HSIPKL+CP G DGVVISTKYLNR L VD EGM I+VEGG 
Sbjct: 77  AVAEATRAMRKIKVGTRFYHSIPKLACPHGVDGVVISTKYLNRVLKVDGEGMRITVEGGV 136

Query: 136 TVRQIIE 143
           TVR+IIE
Sbjct: 137 TVREIIE 139

The following BLAST results are available for this feature:

BLAST of Bhi01G000446 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46760.1	9.8e-36	50.68	D-arabinono-1,4-lactone oxidase family protein	[more]
AT2G46740.1	2.8e-35	52.08	D-arabinono-1,4-lactone oxidase family protein	[more]
AT2G46750.1	5.9e-33	55.74	D-arabinono-1,4-lactone oxidase family protein	[more]
AT5G56490.1	7.7e-33	48.92	D-arabinono-1,4-lactone oxidase family protein	[more]
AT1G32300.1	5.0e-32	49.62	D-arabinono-1,4-lactone oxidase family protein	[more]

BLAST of Bhi01G000446 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81032	1.4e-34	50.68	Probable L-gulonolactone oxidase 6 OS=Arabidopsis thaliana OX=3702 GN=GULLO6 PE=...	[more]
O81030	4.0e-34	52.08	L-gulonolactone oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=GULLO5 PE=1 SV=1	[more]
Q6NQ66	8.3e-32	55.74	L-gulonolactone oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GULLO2 PE=1 SV=1	[more]
Q9FM82	1.1e-31	48.92	Probable L-gulonolactone oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GULLO4 PE=...	[more]
Q9C614	7.1e-31	49.62	Probable L-gulonolactone oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GULLO1 PE=...	[more]

BLAST of Bhi01G000446 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882597.1	6.9e-76	88.30	LOW QUALITY PROTEIN: probable L-gulonolactone oxidase 6 [Benincasa hispida]	[more]
XP_008437774.1	9.7e-62	71.19	PREDICTED: probable L-gulonolactone oxidase 6 [Cucumis melo]	[more]
KAA0048816.1	9.7e-62	71.19	putative L-gulonolactone oxidase 6 [Cucumis melo var. makuwa] >TYK20769.1 putati...	[more]
XP_004134280.1	2.5e-57	69.89	probable L-gulonolactone oxidase 6 [Cucumis sativus] >KAE8650211.1 hypothetical ...	[more]
XP_023540226.1	1.3e-42	73.85	probable L-gulonolactone oxidase 6 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Bhi01G000446 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U3K3	4.7e-62	71.19	Putative L-gulonolactone oxidase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3AUG3	4.7e-62	71.19	L-gulonolactone oxidase OS=Cucumis melo OX=3656 GN=LOC103483108 PE=3 SV=1	[more]
A0A0A0L3K4	1.2e-57	69.89	L-gulonolactone oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G116800 PE=3 SV=1	[more]
A0A6J1IG87	7.1e-42	67.83	L-gulonolactone oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474382 PE=3 SV=1	[more]
A0A6J1FUH8	9.2e-42	74.02	L-gulonolactone oxidase OS=Cucurbita moschata OX=3662 GN=LOC111448536 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 31..161 e-value: 5.3E-8 score: 33.9
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 63..143 e-value: 1.2E-7 score: 31.5
None	No IPR available	PANTHER	PTHR13878	GULONOLACTONE OXIDASE	coord: 9..147
None	No IPR available	PANTHER	PTHR13878:SF93	D-ARABINONO-1,4-LACTONE OXIDASE-LIKE PROTEIN	coord: 9..147
IPR016166	FAD-binding domain, PCMH-type	PROSITE	PS51387	FAD_PCMH	coord: 57..172 score: 10.751824
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 56..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M000446	Bhi01M000446	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019853	L-ascorbic acid biosynthetic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0003885	D-arabinono-1,4-lactone oxidase activity
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0050105	L-gulonolactone oxidase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016491	oxidoreductase activity