Bhi01G000333 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G000333
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	chr1: 8047563 .. 8051178 (+)
RNA-Seq Expression	Bhi01G000333
Synteny	Bhi01G000333

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCAATATGGAACTACGTTCATGCTGGCAAATAAAGTTTTTTCTCTCATTATATAAAGTGGGATGAGGGATTTGAGAATCAACCAAAGATAAGAAAAAGAAGGAAGAAAGCAAAGAAAATGGGAGACTCCAGTAGGTGCAGAGTGTGCGTTACAGGCGGATCAGGGTATGTGGCTTCTCTTCTCATAAAAAAACTTCTTCTAAAGGGTCACATCGTCCATGCAACTCTTCGTAACTTAGGTAACTAAAACTTCAAACTCAAAATCTTAATTAATTAATTATTGTGTGCATCTTGTTCATTCTTGATTAATTTAATTGTTGGATTGTTATTTTTAAGATGATGAATTAAAGGTGAGGGTTTTAAAGAGCCTCCCAAATGCAACCACTAATTTGCTGCTGTTTAAGGCCGACATTTATGAGCCCCATCAGTTTGAAGATGCCATCAGAGGCTGTCGCATCGTTTTCCATGTCGCTACTCCTTTACTCCGCACTCATGGATCTCAGGTTTCTTTAATTTCTTCCTTCCCATCTAAATTTATTATGAAAAAGTTTGTATTTATTTATATGTTGTTTGGTTGAGTCAACGAATCTAAACACACACCAGAAATAGATTTTGAGATTACAATTAGTGCAAAAATCAGATAAACGAAAGAAAATAAAATTTGGTGTTCGGGGTTAAAAGGAGAAAGATGTTATAATATTATATTTATTTCCACACTTTATGTTAAATTAAGTCTTCTTCTATTAGGTAGGTTGAGCTTTATTCGGAGTTTAGTTAGCTAATTTTAGTCTCGTATCAAATTATTTATACCTGTACAATTGTGCTAATTCATCAAGAAATATACATAAAAAAATAATTCACTTAGATCTTGTTCTTCAACATGGTATCAGAGCCTTGCTAGTTGGTTTTCTTCTTTTTTTCCTTGTCTTCTGTTATTTTGTTGCCATCAACCAACCCAGATCTGTTCGACTGTCGAACATGCCGTCCGTGAAGGCGCCAGATCTATCCGATCGTCGAACATGCTGTTCGTGAAGGTGACTCCTCCAACCCAGATCTGTCGCACCAGCTCCGTCCATTCATACATCACCAAGAAGAACCAAATCTGTCCATTCGTTAGTCACACCAATGAAGGCAACCCAAATCTGGCCTCTATCGCACTAAACCTGCGAACGGCTCTGTGACCCGTGAAGAACAACTCCGTGCACTAGACCCGTCCGGCCATTCATTCATCACCAGAACCGTGATGATCCACACGTGCCCATGAGCATCTCCGTTTGTTCATGAGAAATCCATGTCCTGCACGCAACCCGTGAATGGATCTGTGCATACCCATGAGAAAAAAAATCAAGCTGAGTCGTCGAGCTGAGCCCAATCAAGTCGAGCCATCGAGCCGAGAAGTGAGTCGAGCCAACCCTCATTTTTTTAGTCGAGTCGATCCTATTTTTTATATTATTATTTTTTCCCATTATCTAATCCCGTAGATTTTCCTTTCCTTTCCTCTCATTCTAAATGGAGAAAAATGATATTTTTCGTCCTATCAGTACTATCTTCAATAGCACCAACTACATTACTTGGGCATATCAAATAAAGAGCTTCTTGATTGGTCGTGAACTGTGGTAGGTTATGACAGGTGATGACGTTAAACCTGTCAAAGGAGCTTCTGAAGAAGATAATAAGTTTGTGGACAGAATAGAAGACTAGGACAACAAGAACCATCAAATAATCACTCGGACGATTTCAAACCGTGGGCCTGGCATACTACTATCAACTTCACACTACTTTAGTTCGCAAAAGTCAGGAGATCGGGCAATCTATGAATGAGTATCTCGTTATTCTTTAGCCTATTAGAACCCAATTGAACCAGGCTAAGATCAGTCTAGATCATCTATGACTTATCAAGGTCCTTATGGGGCTTTGCTCTGAGTATGAGTTTGTTCGTGTTGCCTTGTTAAATCGCAATCCTCTACCATCTCTTGATGCTGTGATTCAAGAAATTTTCTTTGAGGGGAGGCGATTTAGGTTAGCTTCCTCTCCTTCCCTAGATGTTGCTCTCAAAACCATTCACGATTTCCAACTGAGTCAATTTTCTACAAGAATTGGAAATAACATGGTCACGGGTTTTCCAGTTGCTCTACCATTGAGTGCAGGTACTGCCACAGACGGGGTCATATCCTGGACAACTGTCCTATCAAACCACCTCAGCCTCCAGGATACACCAATAAATCCAATAATTCTTCTAAGTATGGTTCTCCATCTGTCTTGACGGTTAGTACTGCCTCCGATGTGGTACCTCCCAATCCTCTGATTATCCAGATCAACGATCTTCAGGACTTGTTGAAACAAGTGCTTTCTCCCACTTCTCTTGCCCTTACCATCACACCAGGTACATCTTGGCTCCTTAATTCAACTTATTGCAATCACATGACCTTTAATATGGCCTTGTTTTCTTTCTCCACGCTTAGCCAATCCCTGCCTCTGTTATATTCTACTGATGGTAACCACATACCTATTTCACATGTTGGAACTGTTAGTACACCGGGTATTAGTATATCCACTACTTATCATGTCCCACACTTGATCTTTAATCTATGTCTCTGTTGGACAACTGTGTGACTTGGGGTTAACTGTTCTCTTTTTCTCTCATGGTTTTCAGGTTCAAGATCCGCGGACGGGTCAGATGGTTGGCAAGGTCGCAAGATAGGAAGATTATTTGAGCTCACGTCCTTTCAGCCTCCTACTCTGCTATCTGTGTCTACTGCTATTACAGATACCGACACGTATCAGTGGCACCTTCGTTTTGGTCATGCATCATTTGACAAACTTTGTAGCCTAATTTCTATTGGTACCCTAAGTAATGCCTCTCAATTTCGCTCATTTTATAGTTTACATCGCAGGTTGGCAAAACAGCCTGCTTTGTCGTTTCTTACTTCCACATCTCTATGTGATAAACCGTTTGGTCTTATTCATTCTAACATTTGGGGTCCTGCTCGTACCCCTACAATAGATGGTTATTGGTATTTTGTTTTGTTCGTTGATGACTACTCTGGCTTTACTTGGATATATTTTCTTAAGAATCGTTCATCTTTACCCTAAGTTTACATTGAATTTGCTAAAATGATTCAAACTCAGTTTTCTCAAACCATTAAAATTATTCAGACTAACAATGCCACAGAGTATAAGGACTCTCACCTGCTCTCCTTCCTAACCCAACAGGGTAATTTGTCCAATGCTCATGCCCTTATACTCCTCAACAAAATGGTCGGGCTAAAAAAAGGATCGCCATATTCGTGCACAACTTCTCTCTGCATCTTGTCCTGAAAAGTTTTAGGGAGAAGCTGCGCTCACATCTATTTATGTTATCAACTACCTTCCCTCTACTGTTCTCCATAACGTGTCTCCCTTTGAGTGATTGCATAACATTCCTCCCTCTTATTCTTTTTTCAAAGTTTTTGGTTGTGCCTATTTTGTTTTACTTCATCCCCATGAACATTCCAAACTTGAACCTCGGGCTCAGTTGTGTTGCTTCCTTGGTTACGGAATTGAACATAAAGGATTTCGGTGTTGGGATTTGATATCCAATGGGTTACGTATCTCTCACCATGTCACCTTTTGAGA

mRNA sequence

ATTCAATATGGAACTACGTTCATGCTGGCAAATAAAGTTTTTTCTCTCATTATATAAAGTGGGATGAGGGATTTGAGAATCAACCAAAGATAAGAAAAAGAAGGAAGAAAGCAAAGAAAATGGGAGACTCCAGTAGGTGCAGAGTGTGCGTTACAGGCGGATCAGGGTATGTGGCTTCTCTTCTCATAAAAAAACTTCTTCTAAAGGGTCACATCGTCCATGCAACTCTTCGTAACTTAGATGATGAATTAAAGGTGAGGGTTTTAAAGAGCCTCCCAAATGCAACCACTAATTTGCTGCTGTTTAAGGCCGACATTTATGAGCCCCATCAGTTTGAAGATGCCATCAGAGGCTGTCGCATCGTTTTCCATGTCGCTACTCCTTTACTCCGCACTCATGGATCTCAGGTTTCTTTAATTTCTTCCTTCCCATCTAAATTTATTATGAAAAAGTTTGTATTTATTTATATGTTGTTTGGTTGAGTCAACGAATCTAAACACACACCAGAAATAGATTTTGAGATTACAATTAGTGCAAAAATCAGATAAACGAAAGAAAATAAAATTTGGTGTTCGGGGTTAAAAGGAGAAAGATGTTATAATATTATATTTATTTCCACACTTTATGTTAAATTAAGTCTTCTTCTATTAGGTAGGTTGAGCTTTATTCGGAGTTTAGTTAGCTAATTTTAGTCTCGTATCAAATTATTTATACCTGTACAATTGTGCTAATTCATCAAGAAATATACATAAAAAAATAATTCACTTAGATCTTGTTCTTCAACATGGTATCAGAGCCTTGCTAGTTGGTTTTCTTCTTTTTTTCCTTGTCTTCTGTTATTTTGTTGCCATCAACCAACCCAGATCTGTTCGACTGTCGAACATGCCGTCCGTGAAGGCGCCAGATCTATCCGATCGTCGAACATGCTGTTCGTGAAGGTGACTCCTCCAACCCAGATCTGTCGCACCAGCTCCGTCCATTCATACATCACCAAGAAGAACCAAATCTGTCCATTCGTTAGTCACACCAATGAAGGCAACCCAAATCTGGCCTCTATCGCACTAAACCTGCGAACGGCTCTGTGACCCGTGAAGAACAACTCCGTGCACTAGACCCGTCCGGCCATTCATTCATCACCAGAACCGTGATGATCCACACGTGCCCATGAGCATCTCCGTTTGTTCATGAGAAATCCATGTCCTGCACGCAACCCGTGAATGGATCTGTGCATACCCATGAGAAAAAAAATCAAGCTGAGTCGTCGAGCTGAGCCCAATCAAGTCGAGCCATCGAGCCGAGAAGTTATGACAGGTGATGACGTTAAACCTGTCAAAGGAGCTTCTGAAGAAGATAATAAGTTTGTGGACAGAATAGAAGACTAGGACAACAAGAACCATCAAATAATCACTCGGACGATTTCAAACCGTGGGCCTGGCATACTACTATCAACTTCACACTACTTTAGTTCGCAAAAGTCAGGAGATCGGGCAATCTATGAATGAGTATCTCGTTATTCTTTAGCCTATTAGAACCCAATTGAACCAGGCTAAGATCAGTCTAGATCATCTATGACTTATCAAGGTCCTTATGGGGCTTTGCTCTGAGTATGAGTTTGTTCGTGTTGCCTTGTTAAATCGCAATCCTCTACCATCTCTTGATGCTGTGATTCAAGAAATTTTCTTTGAGGGGAGGCGATTTAGGTTAGCTTCCTCTCCTTCCCTAGATGTTGCTCTCAAAACCATTCACGATTTCCAACTGAGTCAATTTTCTACAAGAATTGGAAATAACATGGTCACGGGTTTTCCAGTTGCTCTACCATTGAGTGCAGGTACTGCCACAGACGGGGTCATATCCTGGACAACTGTCCTATCAAACCACCTCAGCCTCCAGGATACACCAATAAATCCAATAATTCTTCTAAGTATGGTTCTCCATCTGTCTTGACGGTTAGTACTGCCTCCGATGTGGTACCTCCCAATCCTCTGATTATCCAGATCAACGATCTTCAGGACTTGTTGAAACAAGTGCTTTCTCCCACTTCTCTTGCCCTTACCATCACACCAGGTTCAAGATCCGCGGACGGGTCAGATGGTTGGCAAGGTCGCAAGATAGGAAGATTATTTGAGCTCACGTCCTTTCAGCCTCCTACTCTGCTATCTGTGTCTACTGCTATTACAGATACCGACACGTATCAGTGGCACCTTCGTTTTGGTCATGCATCATTTGACAAACTTTGTAGCCTAATTTCTATTGGTACCCTAAGTAATGCCTCTCAATTTCGCTCATTTTATAGTTTACATCGCAGGTTGGCAAAACAGCCTGCTTTGTCGTTTCTTACTTCCACATCTCTATGTGATAAACCGTTTGGTCTTATTCATTCTAACATTTGGGGTCCTGCTCGTACCCCTACAATAGATGGTTATTGGTATTTTGTTTTGTTCGTTGATGACTACTCTGGCTTTACTTGGATATATTTTCTTAAGAATCGTTCATCTTTACCCTAAGTTTACATTGAATTTGCTAAAATGATTCAAACTCAGTTTTCTCAAACCATTAAAATTATTCAGACTAACAATGCCACAGAGTATAAGGACTCTCACCTGCTCTCCTTCCTAACCCAACAGGGTAATTTGTCCAATGCTCATGCCCTTATACTCCTCAACAAAATGGTCGGGCTAAAAAAAGGATCGCCATATTCGTGCACAACTTCTCTCTGCATCTTGTCCTGAAAAGTTTTAGGGAGAAGCTGCGCTCACATCTATTTATGTTATCAACTACCTTCCCTCTACTGTTCTCCATAACGTGTCTCCCTTTGAGTGATTGCATAACATTCCTCCCTCTTATTCTTTTTTCAAAGTTTTTGGTTGTGCCTATTTTGTTTTACTTCATCCCCATGAACATTCCAAACTTGAACCTCGGGCTCAGTTGTGTTGCTTCCTTGGTTACGGAATTGAACATAAAGGATTTCGGTGTTGGGATTTGATATCCAATGGGTTACGTATCTCTCACCATGTCACCTTTTGAGA

Coding sequence (CDS)

ATGGGAGACTCCAGTAGGTGCAGAGTGTGCGTTACAGGCGGATCAGGGTATGTGGCTTCTCTTCTCATAAAAAAACTTCTTCTAAAGGGTCACATCGTCCATGCAACTCTTCGTAACTTAGATGATGAATTAAAGGTGAGGGTTTTAAAGAGCCTCCCAAATGCAACCACTAATTTGCTGCTGTTTAAGGCCGACATTTATGAGCCCCATCAGTTTGAAGATGCCATCAGAGGCTGTCGCATCGTTTTCCATGTCGCTACTCCTTTACTCCGCACTCATGGATCTCAGGTTTCTTTAATTTCTTCCTTCCCATCTAAATTTATTATGAAAAAGTTTGTATTTATTTATATGTTGTTTGGTTGA

Protein sequence

MGDSSRCRVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQVSLISSFPSKFIMKKFVFIYMLFG

Homology

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BLAST of Bhi01G000333 vs. TAIR 10
Match: AT1G09510.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 4.9e-18
Identity = 48/93 (51.61%), Postives = 59/93 (63.44%), Query Frame = 0


Query: 9   VCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFKADIYE 68
           VCVTG SGYVAS ++K LLL+G+ V AT+R+  DE K   L +L  A   L LFKAD+ E
Sbjct: 8   VCVTGASGYVASWIVKLLLLRGYTVRATVRDPSDEKKTEHLLALDGAKEKLKLFKADLLE 67

Query: 69  PHQFEDAIRGCRIVFHVATPL-LRTHGSQVSLI 101
              FE AI GC  VFH A+P+ L     Q+ LI
Sbjct: 68  EGSFEQAIEGCDAVFHTASPVSLTVTDPQIELI 100

[Close]

BLAST of Bhi01G000333 vs. TAIR 10
Match: AT1G09480.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 6.4e-18
Identity = 44/84 (52.38%), Postives = 55/84 (65.48%), Query Frame = 0


Query: 9   VCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFKADIYE 68
           VCVTG SGY+AS ++K LLL+G+ V AT+R+L D  K   L +L  A   L LFKAD+ E
Sbjct: 55  VCVTGASGYIASWIVKLLLLRGYTVKATVRDLTDRKKTEHLLALDGAKERLKLFKADLLE 114

Query: 69  PHQFEDAIRGCRIVFHVATPLLRT 93
              FE AI GC  VFH A+P+  T
Sbjct: 115 ESSFEQAIEGCDAVFHTASPVFFT 138

[Close]

BLAST of Bhi01G000333 vs. TAIR 10
Match: AT1G09490.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 85.9 bits (211), Expect = 2.4e-17
Identity = 43/84 (51.19%), Postives = 57/84 (67.86%), Query Frame = 0


Query: 9  VCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFKADIYE 68
          VCVTG SGY+AS ++K LLL+G+ V+AT+R+  D+ K   L +L  A   L LFKAD+ E
Sbjct: 8  VCVTGASGYIASWIVKLLLLRGYTVNATVRDPKDKKKTEHLLALDGAKERLKLFKADLLE 67

Query: 69 PHQFEDAIRGCRIVFHVATPLLRT 93
             F+ AI GC  VFH A+P+L T
Sbjct: 68 ESSFDQAIDGCDAVFHTASPVLFT 91

[Close]

BLAST of Bhi01G000333 vs. TAIR 10
Match: AT5G42800.1 (dihydroflavonol 4-reductase )

HSP 1 Score: 84.7 bits (208), Expect = 5.4e-17
Identity = 43/86 (50.00%), Postives = 59/86 (68.60%), Query Frame = 0


Query: 4  SSRCRVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFK 63
          S +  VCVTG SG++ S L+ +LL +G+ V AT+R+  +  KV+ L  LPNA T L L+K
Sbjct: 3  SQKETVCVTGASGFIGSWLVMRLLERGYFVRATVRDPGNLKKVQHLLDLPNAKTLLTLWK 62

Query: 64 ADIYEPHQFEDAIRGCRIVFHVATPL 90
          AD+ E   ++DAI GC  VFHVATP+
Sbjct: 63 ADLSEEGSYDDAINGCDGVFHVATPM 88

[Close]

BLAST of Bhi01G000333 vs. TAIR 10
Match: AT1G66800.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 2.1e-16
Identity = 42/84 (50.00%), Postives = 55/84 (65.48%), Query Frame = 0


Query: 9  VCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFKADIYE 68
          VCVTG SGY+AS ++K LLL+G+ V AT+RN  D  K   L +L  A+  L LFK+D+ E
Sbjct: 8  VCVTGASGYIASWIVKLLLLRGYTVRATVRNPMDTKKTNHLLTLEGASERLKLFKSDLLE 67

Query: 69 PHQFEDAIRGCRIVFHVATPLLRT 93
             F+ AI GC  VFH A+P+  T
Sbjct: 68 EGSFDQAIEGCDGVFHTASPVSLT 91

[Close]

BLAST of Bhi01G000333 vs. ExPASy Swiss-Prot
Match: P51105 (Dihydroflavonol 4-reductase OS=Gerbera hybrida OX=18101 GN=DFR PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 8.1e-18
Identity = 43/89 (48.31%), Postives = 63/89 (70.79%), Query Frame = 0


Query: 1  MGDSSRCRVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLL 60
          M + S   VCVTG +G++ S L+ +LL +G++VHAT+R+  D  KV+ L  LP A TNL 
Sbjct: 1  MEEDSPATVCVTGAAGFIGSWLVMRLLERGYVVHATVRDPGDLKKVKHLLELPKAQTNLK 60

Query: 61 LFKADIYEPHQFEDAIRGCRIVFHVATPL 90
          L+KAD+ +   F++AI+GC  VFH+ATP+
Sbjct: 61 LWKADLTQEGSFDEAIQGCHGVFHLATPM 89

[Close]

BLAST of Bhi01G000333 vs. ExPASy Swiss-Prot
Match: P51103 (Dihydroflavonol 4-reductase OS=Callistephus chinensis OX=13379 GN=F PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-17
Identity = 45/89 (50.56%), Postives = 61/89 (68.54%), Query Frame = 0


Query: 1  MGDSSRCRVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLL 60
          M + S   VCVTG +G++ S L+ +LL +G+IV AT+RN  D  KV+ L  LP A TNL 
Sbjct: 1  MKEDSPPTVCVTGAAGFIGSWLVMRLLERGYIVRATVRNPGDMKKVKHLLELPKAETNLT 60

Query: 61 LFKADIYEPHQFEDAIRGCRIVFHVATPL 90
          L+KAD+ +   F++AI GC  VFHVATP+
Sbjct: 61 LWKADLTQEGSFDEAIEGCHGVFHVATPM 89

[Close]

BLAST of Bhi01G000333 vs. ExPASy Swiss-Prot
Match: Q5XLY0 (Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.4e-17
Identity = 43/80 (53.75%), Postives = 60/80 (75.00%), Query Frame = 0


Query: 9  VCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFKADIYE 68
          VCVTG +G++AS L+K+LL KG+IVHAT+R+ +++ KV  L +LP AT  L LF+A++ E
Sbjct: 15 VCVTGAAGFMASWLVKRLLEKGYIVHATVRDPENKAKVSHLLNLPGATDRLKLFRAELCE 74

Query: 69 PHQFEDAIRGCRIVFHVATP 89
             F+ A+ GC  VFHVATP
Sbjct: 75 DGSFDAAVAGCNGVFHVATP 94

[Close]

BLAST of Bhi01G000333 vs. ExPASy Swiss-Prot
Match: P51104 (Dihydroflavonol 4-reductase OS=Dianthus caryophyllus OX=3570 GN=A PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.4e-17
Identity = 43/81 (53.09%), Postives = 59/81 (72.84%), Query Frame = 0


Query: 9   VCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFKADIYE 68
           VCVTG SG++ S LI +LL +G+ V AT+R+ D+  KV+ L  LPNA TNL L+KAD++E
Sbjct: 25  VCVTGASGFIGSWLIMRLLERGYTVRATVRDPDNTKKVQHLLDLPNAKTNLTLWKADLHE 84

Query: 69  PHQFEDAIRGCRIVFHVATPL 90
              F+ A+ GC  VFH+ATP+
Sbjct: 85  EGSFDAAVDGCTGVFHIATPM 105

[Close]

BLAST of Bhi01G000333 vs. ExPASy Swiss-Prot
Match: P14720 (Dihydroflavonol 4-reductase OS=Petunia hybrida OX=4102 GN=DFRA PE=2 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 9.0e-17
Identity = 41/81 (50.62%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 9  VCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFKADIYE 68
          VCVTG +G++ S L+ +LL +G+ VHAT+R+ +++ KV+ L  LP A TNL L+KAD+  
Sbjct: 18 VCVTGAAGFIGSWLVMRLLERGYNVHATVRDPENKKKVKHLLELPKADTNLTLWKADLTV 77

Query: 69 PHQFEDAIRGCRIVFHVATPL 90
             F++AI+GC+ VFHVATP+
Sbjct: 78 EGSFDEAIQGCQGVFHVATPM 98

[Close]

BLAST of Bhi01G000333 vs. NCBI nr
Match: XP_038896232.1 (putative anthocyanidin reductase [Benincasa hispida])

HSP 1 Score: 196.8 bits (499), Expect = 1.1e-46
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 0


Query: 1  MGDSSRCRVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLL 60
          MGDSSRCRVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLL
Sbjct: 1  MGDSSRCRVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLL 60

Query: 61 LFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQ 97
          LFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQ
Sbjct: 61 LFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQ 96

[Close]

BLAST of Bhi01G000333 vs. NCBI nr
Match: XP_008437918.1 (PREDICTED: vestitone reductase-like [Cucumis melo] >KAA0048905.1 vestitone reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 157.1 bits (396), Expect = 9.2e-35
Identity = 78/99 (78.79%), Postives = 84/99 (84.85%), Query Frame = 0


Query: 1   MGDSSRC-RVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNL 60
           +  SS C RVCVTGGSGYVAS LIKKLLL GHIVHATLRNLDDE K+ +LK LPNATTNL
Sbjct: 3   VSSSSGCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATTNL 62

Query: 61  LLFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQVS 99
           +LFKADIYEPH+FE AIRGC IVFHVATPL  TH +Q S
Sbjct: 63  VLFKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYS 101

[Close]

BLAST of Bhi01G000333 vs. NCBI nr
Match: XP_038874896.1 (putative anthocyanidin reductase [Benincasa hispida])

HSP 1 Score: 155.6 bits (392), Expect = 2.7e-34
Identity = 75/88 (85.23%), Postives = 79/88 (89.77%), Query Frame = 0


Query: 9  VCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFKADIYE 68
          VCVTGGSGYVAS LIKKLLLKGHIVHAT+RNLDDE K  +LK LPNATTNL+LFKADIYE
Sbjct: 3  VCVTGGSGYVASSLIKKLLLKGHIVHATVRNLDDESKAGILKRLPNATTNLVLFKADIYE 62

Query: 69 PHQFEDAIRGCRIVFHVATPLLRTHGSQ 97
          PHQFE AIRGC IVFHVATPL  THG+Q
Sbjct: 63 PHQFEAAIRGCHIVFHVATPLHHTHGTQ 90

[Close]

BLAST of Bhi01G000333 vs. NCBI nr
Match: XP_004134310.3 (putative anthocyanidin reductase isoform X1 [Cucumis sativus] >KAE8650235.1 hypothetical protein Csa_010630 [Cucumis sativus])

HSP 1 Score: 153.3 bits (386), Expect = 1.3e-33
Identity = 78/97 (80.41%), Postives = 81/97 (83.51%), Query Frame = 0


Query: 1  MGDSSRC-RVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNL 60
          M  SS C RVCVTGGSGYVAS LIKKLLL GHIVHATLRNL DE KV +LK LPNAT NL
Sbjct: 1  MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60

Query: 61 LLFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQ 97
          +LFKADIYEPHQFE AIRGC IVFHVATPL  TH +Q
Sbjct: 61 VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQ 97

[Close]

BLAST of Bhi01G000333 vs. NCBI nr
Match: XP_031737949.1 (putative anthocyanidin reductase isoform X2 [Cucumis sativus])

HSP 1 Score: 153.3 bits (386), Expect = 1.3e-33
Identity = 78/97 (80.41%), Postives = 81/97 (83.51%), Query Frame = 0


Query: 1  MGDSSRC-RVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNL 60
          M  SS C RVCVTGGSGYVAS LIKKLLL GHIVHATLRNL DE KV +LK LPNAT NL
Sbjct: 1  MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60

Query: 61 LLFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQ 97
          +LFKADIYEPHQFE AIRGC IVFHVATPL  TH +Q
Sbjct: 61 VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQ 97

[Close]

BLAST of Bhi01G000333 vs. ExPASy TrEMBL
Match: A0A5A7U5J5 (Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001230 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.5e-35
Identity = 78/99 (78.79%), Postives = 84/99 (84.85%), Query Frame = 0


Query: 1   MGDSSRC-RVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNL 60
           +  SS C RVCVTGGSGYVAS LIKKLLL GHIVHATLRNLDDE K+ +LK LPNATTNL
Sbjct: 3   VSSSSGCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATTNL 62

Query: 61  LLFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQVS 99
           +LFKADIYEPH+FE AIRGC IVFHVATPL  TH +Q S
Sbjct: 63  VLFKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYS 101

[Close]

BLAST of Bhi01G000333 vs. ExPASy TrEMBL
Match: A0A1S3AUS8 (vestitone reductase-like OS=Cucumis melo OX=3656 GN=LOC103483203 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.5e-35
Identity = 78/99 (78.79%), Postives = 84/99 (84.85%), Query Frame = 0


Query: 1   MGDSSRC-RVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNL 60
           +  SS C RVCVTGGSGYVAS LIKKLLL GHIVHATLRNLDDE K+ +LK LPNATTNL
Sbjct: 3   VSSSSGCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATTNL 62

Query: 61  LLFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQVS 99
           +LFKADIYEPH+FE AIRGC IVFHVATPL  TH +Q S
Sbjct: 63  VLFKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYS 101

[Close]

BLAST of Bhi01G000333 vs. ExPASy TrEMBL
Match: A0A0A0L371 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119707 PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 6.4e-34
Identity = 78/97 (80.41%), Postives = 81/97 (83.51%), Query Frame = 0


Query: 1  MGDSSRC-RVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNL 60
          M  SS C RVCVTGGSGYVAS LIKKLLL GHIVHATLRNL DE KV +LK LPNAT NL
Sbjct: 1  MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60

Query: 61 LLFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQ 97
          +LFKADIYEPHQFE AIRGC IVFHVATPL  TH +Q
Sbjct: 61 VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQ 97

[Close]

BLAST of Bhi01G000333 vs. ExPASy TrEMBL
Match: A0A5D3D2U9 (Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G005360 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.4e-33
Identity = 76/99 (76.77%), Postives = 82/99 (82.83%), Query Frame = 0


Query: 1   MGDSSRC-RVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNL 60
           +  SS C RVCVT GSGYVAS  IKKLLL GHIVHATLRNLDDE K+ +LK LPNATTNL
Sbjct: 3   VSSSSGCLRVCVTCGSGYVASSFIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATTNL 62

Query: 61  LLFKADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQVS 99
           +LFKADIYEPH+FE AIRGC IVFHVATPL  TH +Q S
Sbjct: 63  VLFKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYS 101

[Close]

BLAST of Bhi01G000333 vs. ExPASy TrEMBL
Match: A0A5D3D1Q4 (Vestitone reductase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G005370 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 7.9e-32
Identity = 71/93 (76.34%), Postives = 77/93 (82.80%), Query Frame = 0


Query: 4  SSRCRVCVTGGSGYVASLLIKKLLLKGHIVHATLRNLDDELKVRVLKSLPNATTNLLLFK 63
          S  CRVCVTGGSGYVA+ LIKKLL  GHIVH TLRNLDDE KV VLKSLPNA TNL+LF+
Sbjct: 2  SGCCRVCVTGGSGYVAASLIKKLLQNGHIVHTTLRNLDDESKVGVLKSLPNAATNLVLFE 61

Query: 64 ADIYEPHQFEDAIRGCRIVFHVATPLLRTHGSQ 97
          ADIY+PHQFE AI G   VFH+ATPL  THG+Q
Sbjct: 62 ADIYKPHQFEAAITGTNFVFHIATPLHHTHGTQ 94

The following BLAST results are available for this feature:

BLAST of Bhi01G000333 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09510.1	4.9e-18	51.61	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G09480.1	6.4e-18	52.38	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G09490.1	2.4e-17	51.19	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G42800.1	5.4e-17	50.00	dihydroflavonol 4-reductase	[more]
AT1G66800.1	2.1e-16	50.00	NAD(P)-binding Rossmann-fold superfamily protein	[more]

BLAST of Bhi01G000333 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51105	8.1e-18	48.31	Dihydroflavonol 4-reductase OS=Gerbera hybrida OX=18101 GN=DFR PE=2 SV=1	[more]
P51103	1.1e-17	50.56	Dihydroflavonol 4-reductase OS=Callistephus chinensis OX=13379 GN=F PE=2 SV=1	[more]
Q5XLY0	2.4e-17	53.75	Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1	[more]
P51104	2.4e-17	53.09	Dihydroflavonol 4-reductase OS=Dianthus caryophyllus OX=3570 GN=A PE=2 SV=1	[more]
P14720	9.0e-17	50.62	Dihydroflavonol 4-reductase OS=Petunia hybrida OX=4102 GN=DFRA PE=2 SV=2	[more]

BLAST of Bhi01G000333 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896232.1	1.1e-46	100.00	putative anthocyanidin reductase [Benincasa hispida]	[more]
XP_008437918.1	9.2e-35	78.79	PREDICTED: vestitone reductase-like [Cucumis melo] >KAA0048905.1 vestitone reduc...	[more]
XP_038874896.1	2.7e-34	85.23	putative anthocyanidin reductase [Benincasa hispida]	[more]
XP_004134310.3	1.3e-33	80.41	putative anthocyanidin reductase isoform X1 [Cucumis sativus] >KAE8650235.1 hypo...	[more]
XP_031737949.1	1.3e-33	80.41	putative anthocyanidin reductase isoform X2 [Cucumis sativus]	[more]

BLAST of Bhi01G000333 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U5J5	4.5e-35	78.79	Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A1S3AUS8	4.5e-35	78.79	vestitone reductase-like OS=Cucumis melo OX=3656 GN=LOC103483203 PE=3 SV=1	[more]
A0A0A0L371	6.4e-34	80.41	Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119707 P...	[more]
A0A5D3D2U9	1.4e-33	76.77	Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5D3D1Q4	7.9e-32	76.34	Vestitone reductase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008030	NmrA-like domain	PFAM	PF05368	NmrA	coord: 9..90 e-value: 1.6E-7 score: 31.1
None	No IPR available	GENE3D	3.40.50.720		coord: 4..101 e-value: 2.0E-27 score: 98.2
None	No IPR available	PANTHER	PTHR10366	NAD DEPENDENT EPIMERASE/DEHYDRATASE	coord: 4..93
None	No IPR available	PANTHER	PTHR10366:SF718	ANTHOCYANIDIN REDUCTASE-LIKE	coord: 4..93
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 6..91

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M000333	Bhi01M000333	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006694	steroid biosynthetic process
molecular_function	GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity