Bhi01G000051 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000051
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptiontRNA (Guanine-N(7)-)-methyltransferase
Locationchr1: 1483791 .. 1486825 (+)
RNA-Seq ExpressionBhi01G000051
SyntenyBhi01G000051
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCGTCCTTGTGCTTGCATTTACAAACTTGCTCACACCTCCGTTACATTGCGGCTGCAAAGGCGGCGGCGCCGTCGAGGGTTGCAACTGCAAACAAAACCTTATCTCTGTTCCTAAAACGACCATTTCTTCCAAACTTCTGTTTACCATCGGGCTCTTCCGCCGACCTTTGCGAGCGTCAACCCCTCCGGAGTCCGGAATTGGTGGCGTTGGAATATGCCGACCTTAATCTCTCTCACAAGATCTTCGAGGTTTGTGTCTCTGTTCATGAATTTTGGCCCTAATTTCTGAAAACCTTGGCGCGTAATTGAAAATTGAATGTGCTGTTTATAATTGATGCGCATTTTTCTTGGTTTTCTTATAATTGCAGGATTCCGGCCACGTTAGAATCAGGCAGCATGTTAATCCTCTTAGCTCTTCTTTCTCTGTATGTAATTTGAAGAGTGATTTAAGGAACTGTGGGTTTGAAATTTGAGCTTAGTGTTGCTTAATTGAAGGAAGGCTTTCCCTCTTTTACCTTTCAGGTCCCTACACCAGCACCAAACTGGGATGAAGTCTTCAAGGACCCTACATTGCCCCTCATGGTTGATATAGGATGTGGTCAGTGAGTTGACGCATTCTCTGTTATGTACCTATAAATATCTGTTTTCTTCATAATACCTACTTTTTTTGTATCCCAGTTACTTATGCATACTTTAATTTCATGAGACAATAATAATATTTGATTCTCTATAACATTTCGCTGCATCCATGTCTGTTATGAAGTGTTTAGGACTGAGAGTGATTTTGAAGGGTTAAAAATCATGTTTGTCATGTTCAAATGCCCTATAAAATATGCTTTTAGTCATTCAAAATCAAATTAGATCGTATGAAAATAGTGTTTAAAAATATAAAATTAAACAATAAATTGATTTTAAATAGTCAAAGACATGTTCGGGAGTGATTTTGAACATGAAAAAGTGATTTTCCAAAATCACCCCGTTATAATTGTCCATCTCTTACCCATTATCCTCCAGCTTGGGCTAAGCAGAGGATAGTCATGAAAAAATCTTTGAAGTTGCTGCTTGCTTTGAAAAAGAAGTTCAAAAGACAGATATATTTAGAGATCCTGCTATTTTCTTAGTCTTGTTTTGATGATGTTCAGTCAACCAGCAGAAGTCATACAAACCAAGTAAAAGGGTAATTTGGCTGGTGAGAAAATTGTATCAAACGACTATGAATATCCTCAAAGGGAAGTATTATCAAGCATCATATTGATTACTGCACTCGGCAATCTTATTTTCCTTGCACTTTGACTCAAAATTCGAATTTATATGCATAATAAAGCAACTCTAATTATGAGACAGTACGGTACATGTGGAGAAAAATACTCTAAGCAATAGAAGCTATGAGCTACCCACCTTGATTTAAACACTGGGCTAGCTAGTAAAAGTTAATATAATATCTATGAAATGTTTCTTTTACCAAAAAAAAGTTAATATAATACCAGTTGCCTAAGTTGACCTGGAGAATTTTCAGAGGAAAGGATTCTATTGGTAAAGAGATTATGGTTTCAAATGTAATCGAGCATATCCCATGTATACATGAATATTATTTGTCAGCTATGCTGTAATGATCTACATTGCATCTTTACTTTTAAAAATGTTAGTACAATTGATGGAAATCTTGATTCTCTCTCAATTTTGGTTTAACTAGCCTCATTACATGCTTTTTATGGGATTCATGAGGCTGTTTTATACTTTCTTTGCCAATCAAGACTCTAACAGAACAAGTATTCATATTCCCAGGTAGTGGCAGATTTCTCTTGTGGCTTGCAAAAAGGCACTTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGAGAGAAAGTACGGGAGAATTTTCACTTATGACGTACTCTTAATTGCTCGGAATTATTTGATCCTCATTCTCTGGAGACCTTAGATTATACTTTGCTTTATTTTGTGCTCTTGCAGCTGGTCAAGCGTGCTGATATCTGGGTTAAAGAGTTGGGATTTAGCAACGTGTAAGGATCTTAACTTCATATCAGGTAGTGAAAAGCAAGATCACCTTTTTGCTATTGGTGTATTATTAATGAACTTCTTTTTAAACTTGAATGCAGACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATTATATCATCATATCCTGGACCCCTGATGCTGGTTTCCATCCTGGTTAGTGCACAATCATTCATACTTTCATAGCACAATACATGCATATTTGGGATGATTTTAAAATGGTTAAAATTACTCTTGTCATGTTTAAAATCAATTTGAAATATACCTTTAGTCACTTGAAATTGACTCAATGTTCACTTTTAGATGTATTTTTCGTACTATTACAATTGATTTTGAATGGTTAAAACCATATTTAGAGTGATTTTGAAAATGACAAAAGTGATTTAAACCATTTTAAAATCACTCCCAAACATATATTGTGATATTTTTTCAACCCCCGCCCAAAACAAGTCAACCCAAGCTTCCCCATTGATTCAATCCAATTAATCGACAACGATATAACTCACTCGTGTTTCATCTCATTGCAGTGCCCAGATCCTCATTTCAAGAAACGACATCATAAGCGAAGGGTTTTGCAGAAGCCTTTGGTAGAATCTATAGTAGACAGTTTAATGCCAGGCGGGCAGGTATATGATCGCATTATCCATCCCTGACATTGAATGGCTTTTTGCTCGCGCTGTTTCAGCCTGACGTAATGCAATATGATGCAATCAGATGAGTCTGATGTTGGAAATTTTGTTTTATATGATTCAATAGGTTTTCATACAATCCGACGTGCTCCAAGTGGCAGTTGACATGAGACTTTTGTTCGATTCCAAAGCCGATGTTCTCAAACACATCGACAAGCTTGACCAGAGTCTTTTGTGTGATGAAGATGGATGGTTGTTGAACAATCCAATGGGAATAAGGACTGAAAGAGAAATTCACGCTGAATCAGAAGGTGCAAACATTTATAGAAGAATGTATCAAAAGTATAATTGA

mRNA sequence

ATGGCTTCGTCCTTGTGCTTGCATTTACAAACTTGCTCACACCTCCGTTACATTGCGGCTGCAAAGGCGGCGGCGCCGTCGAGGGTTGCAACTGCAAACAAAACCTTATCTCTGTTCCTAAAACGACCATTTCTTCCAAACTTCTGTTTACCATCGGGCTCTTCCGCCGACCTTTGCGAGCGTCAACCCCTCCGGAGTCCGGAATTGGTGGCGTTGGAATATGCCGACCTTAATCTCTCTCACAAGATCTTCGAGGATTCCGGCCACGTTAGAATCAGGCAGCATGTTAATCCTCTTAGCTCTTCTTTCTCTGTCCCTACACCAGCACCAAACTGGGATGAAGTCTTCAAGGACCCTACATTGCCCCTCATGGTTGATATAGGATGTGGTCATGGCAGATTTCTCTTGTGGCTTGCAAAAAGGCACTTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGAGAGAAACTGGTCAAGCGTGCTGATATCTGGGTTAAAGAGTTGGGATTTAGCAACGTACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATTATATCATCATATCCTGGACCCCTGATGCTGGTTTCCATCCTGTGCCCAGATCCTCATTTCAAGAAACGACATCATAAGCGAAGGGTTTTGCAGAAGCCTTTGGTAGAATCTATAGTAGACAGTTTAATGCCAGGCGGGCAGGTTTTCATACAATCCGACGTGCTCCAAGTGGCAGTTGACATGAGACTTTTGTTCGATTCCAAAGCCGATGTTCTCAAACACATCGACAAGCTTGACCAGAGTCTTTTGTGTGATGAAGATGGATGGTTGTTGAACAATCCAATGGGAATAAGGACTGAAAGAGAAATTCACGCTGAATCAGAAGGTGCAAACATTTATAGAAGAATGTATCAAAAGTATAATTGA

Coding sequence (CDS)

ATGGCTTCGTCCTTGTGCTTGCATTTACAAACTTGCTCACACCTCCGTTACATTGCGGCTGCAAAGGCGGCGGCGCCGTCGAGGGTTGCAACTGCAAACAAAACCTTATCTCTGTTCCTAAAACGACCATTTCTTCCAAACTTCTGTTTACCATCGGGCTCTTCCGCCGACCTTTGCGAGCGTCAACCCCTCCGGAGTCCGGAATTGGTGGCGTTGGAATATGCCGACCTTAATCTCTCTCACAAGATCTTCGAGGATTCCGGCCACGTTAGAATCAGGCAGCATGTTAATCCTCTTAGCTCTTCTTTCTCTGTCCCTACACCAGCACCAAACTGGGATGAAGTCTTCAAGGACCCTACATTGCCCCTCATGGTTGATATAGGATGTGGTCATGGCAGATTTCTCTTGTGGCTTGCAAAAAGGCACTTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGAGAGAAACTGGTCAAGCGTGCTGATATCTGGGTTAAAGAGTTGGGATTTAGCAACGTACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATTATATCATCATATCCTGGACCCCTGATGCTGGTTTCCATCCTGTGCCCAGATCCTCATTTCAAGAAACGACATCATAAGCGAAGGGTTTTGCAGAAGCCTTTGGTAGAATCTATAGTAGACAGTTTAATGCCAGGCGGGCAGGTTTTCATACAATCCGACGTGCTCCAAGTGGCAGTTGACATGAGACTTTTGTTCGATTCCAAAGCCGATGTTCTCAAACACATCGACAAGCTTGACCAGAGTCTTTTGTGTGATGAAGATGGATGGTTGTTGAACAATCCAATGGGAATAAGGACTGAAAGAGAAATTCACGCTGAATCAGAAGGTGCAAACATTTATAGAAGAATGTATCAAAAGTATAATTGA

Protein sequence

MASSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSADLCERQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGANIYRRMYQKYN
Homology
BLAST of Bhi01G000051 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 365.5 bits (937), Expect = 4.1e-101
Identity = 189/315 (60.00%), Postives = 230/315 (73.02%), Query Frame = 0

Query: 3   SSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFC----------LPS 62
           +SLC   Q CS     +A       RV +   +  +F++   +P  C            S
Sbjct: 2   ASLC---QACSS---SSATPLLLAKRVFSLASSPFIFMREHKIPRHCNSFRAFNAVSASS 61

Query: 63  GSSADLC-ERQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPN 122
            +S  +C + + LRS ELV LEYA+LNL+HKI E+ GHVRIRQHVNPLSSSFS P P P 
Sbjct: 62  SASTSVCVDFKDLRSNELVELEYAELNLNHKISEEVGHVRIRQHVNPLSSSFSKPAPVPV 121

Query: 123 WDEVFKDPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFS 182
           WDEV+KDP+LPLMVDIG G GRFLLW A ++ +SRN+LGLEIR+KLVKRA+ WV ELG S
Sbjct: 122 WDEVYKDPSLPLMVDIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGLS 181

Query: 183 NVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMP 242
           NVHF+FANA +SF+ +ISSYPGPL +VSILCPDPHFKKRH KRRV+QKPLV SI+ +L P
Sbjct: 182 NVHFIFANAMVSFEHLISSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQNLKP 241

Query: 243 GGQVFIQSDVLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIH 302
           GG++F+QSDVL VA DMR   D +++VL+H+D +D      EDGWL  NPMGIRTEREIH
Sbjct: 242 GGKIFVQSDVLDVAQDMRDQLDEESNVLQHMDTVD-----TEDGWLTENPMGIRTEREIH 301

Query: 303 AESEGANIYRRMYQK 307
           AE EGA IYRR+YQK
Sbjct: 302 AEFEGARIYRRLYQK 305

BLAST of Bhi01G000051 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-11
Identity = 51/175 (29.14%), Postives = 82/175 (46.86%), Query Frame = 0

Query: 91  RIRQHVNPLSSS-FSVPTPAPNWDEVFKDPTL----------PLMVDIGCGHGRFLLWLA 150
           R R H NPLS S F +P    + D     P                DIGCG G  L+ LA
Sbjct: 23  RARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKFIKKVEFADIGCGFGGLLISLA 82

Query: 151 KRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMFANATI----SFKQIISSY-PGP 210
              F     +G+E+R+K+ +     +  L  ++    + N ++    S K I + +  G 
Sbjct: 83  TL-FPDTLMIGMELRDKVTEYVKERILALRRTSSEGQYENISVVRTNSMKYIPNYFEKGQ 142

Query: 211 LMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLQVAVDMR 250
           L  +  L PDPHFK+++H+RRV+   L++     L  GG ++  +DV ++   M+
Sbjct: 143 LSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTITDVEELGEWMK 196

BLAST of Bhi01G000051 vs. ExPASy Swiss-Prot
Match: Q2JJQ0 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=trmB PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.1e-42
Identity = 99/214 (46.26%), Postives = 129/214 (60.28%), Query Frame = 0

Query: 88  GHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGCGHGRFLLWLAKRHFDSRN 147
           GH R+RQHVNPL + +  P P P+W+ V++    P  +DIG G GRFLL +A+   D  N
Sbjct: 35  GH-RVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRFLLRIAQEQPD-WN 94

Query: 148 FLGLEIREKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHF 207
           FLG+EIR+ LV+RA+ W  ELG  NVHF+FAN  +S + + +  PG L  V+IL PDP F
Sbjct: 95  FLGVEIRQALVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTILFPDPWF 154

Query: 208 KKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLQVAVDMRLLFDSKADVLKHIDKLDQ 267
           KKRHHKRR++Q  LV  +   L PG  VF+QSD+ +VA +M   F      L+H    D 
Sbjct: 155 KKRHHKRRIVQPQLVADLALLLRPGSPVFLQSDIQEVAEEMAARF------LEHPQFWD- 214

Query: 268 SLLCDEDGWLLNNPMGIRTEREIHAESEGANIYR 302
                  G L +NP+GI TERE         IYR
Sbjct: 215 ----PHQGPLDSNPLGIPTEREWQCLQLDLPIYR 233

BLAST of Bhi01G000051 vs. ExPASy Swiss-Prot
Match: Q2JRH1 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=trmB PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.5e-42
Identity = 98/211 (46.45%), Postives = 127/211 (60.19%), Query Frame = 0

Query: 91  RIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFLG 150
           R+RQHVNPL + +  PTP P+W+ V++    PL +DIG G GRFLL +A+   D  NFLG
Sbjct: 34  RLRQHVNPLQAQYQQPTPPPHWERVYRRLGQPLHLDIGSGSGRFLLRMAQEQPD-WNFLG 93

Query: 151 LEIREKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKR 210
           +EIR+ LV+RA+ W  ELG  NVHF+FAN  +S + + +  PG L  V+I  PDP FKKR
Sbjct: 94  VEIRQPLVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTIQFPDPWFKKR 153

Query: 211 HHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLQVAVDMRLLFDSKADVLKHIDKLDQSLL 270
           HHKRR++Q  LV  +   L PG  VF+QSDV +VA +M   F      L+H         
Sbjct: 154 HHKRRIVQPRLVADLALLLQPGSPVFLQSDVREVAEEMVDRF------LEH-----PQFW 213

Query: 271 CDEDGWLLNNPMGIRTEREIHAESEGANIYR 302
               G + +NP GI TERE      G  IYR
Sbjct: 214 NPYQGPIDDNPFGIPTEREWQCLQLGLPIYR 230

BLAST of Bhi01G000051 vs. ExPASy Swiss-Prot
Match: P73161 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=trmB PE=3 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 2.1e-41
Identity = 94/217 (43.32%), Postives = 127/217 (58.53%), Query Frame = 0

Query: 90  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFL 149
           VRIRQHVNPLS  +      P+W  V+ D   PL +DIGC  GRF L +A++H D  NFL
Sbjct: 4   VRIRQHVNPLSEKYRQVLACPDWATVYDDVQRPLHLDIGCARGRFPLKMAQQHPD-WNFL 63

Query: 150 GLEIREKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKK 209
           G+EIR+ LV  A+     LG  N+H++F N  +  ++  S++P  L  VSI  PDP FK+
Sbjct: 64  GVEIRQPLVLEANETGDRLGLKNLHYLFGNINVEPEKFFSAFPPTLQRVSIQFPDPWFKQ 123

Query: 210 RHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLQVAVDMRLLFDSKADVLKHIDKLDQSL 269
           RH+KRRV Q  LV +I ++L PGG+V +QSDV  VA DMR  F             D  +
Sbjct: 124 RHNKRRVAQPELVTAIANALPPGGEVLLQSDVEPVAQDMRDRFAEN----------DNFV 183

Query: 270 LCDEDGWLLNNPMGIRTEREIHAESEGANIYRRMYQK 307
              +  WL  NP+G+ TEREI   +    +YR + Q+
Sbjct: 184 FTHDTPWLAENPLGVPTEREIACFNLQRPVYRCLLQR 209

BLAST of Bhi01G000051 vs. ExPASy Swiss-Prot
Match: P72546 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=trmB PE=3 SV=2)

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-39
Identity = 91/216 (42.13%), Postives = 131/216 (60.65%), Query Frame = 0

Query: 90  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFL 149
           VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIGC  GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 150 GLEIREKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 209
           GLEIRE LV  A+   +E   +N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 210 KRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLQVAVDMRLLFDSKADVLKHIDKLDQS 269
           KRH KRRV+Q  LV+++  +L  G +VF+QSDVL+V  +M   F ++        +  ++
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAE-------PRFQRT 183

Query: 270 LLCDEDGWLLNNPMGIRTEREIHAESEGANIYRRMY 305
            L     WL  NP+ + TEREI  +++   +YR ++
Sbjct: 184 CL----DWLPENPLPVPTEREIAVQNKQLPVYRALF 207

BLAST of Bhi01G000051 vs. ExPASy Swiss-Prot
Match: Q5N2X5 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=trmB PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-39
Identity = 91/216 (42.13%), Postives = 131/216 (60.65%), Query Frame = 0

Query: 90  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFL 149
           VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIGC  GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 150 GLEIREKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 209
           GLEIRE LV  A+   +E   +N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 210 KRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLQVAVDMRLLFDSKADVLKHIDKLDQS 269
           KRH KRRV+Q  LV+++  +L  G +VF+QSDVL+V  +M   F ++        +  ++
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAE-------PRFQRT 183

Query: 270 LLCDEDGWLLNNPMGIRTEREIHAESEGANIYRRMY 305
            L     WL  NP+ + TEREI  +++   +YR ++
Sbjct: 184 CL----DWLPENPLPVPTEREIAVQNKQLPVYRALF 207

BLAST of Bhi01G000051 vs. NCBI nr
Match: XP_038879912.1 (tRNA (guanine-N(7)-)-methyltransferase [Benincasa hispida])

HSP 1 Score: 624.4 bits (1609), Expect = 5.2e-175
Identity = 307/308 (99.68%), Postives = 307/308 (99.68%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSADLCE 60
           MASSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSADLCE
Sbjct: 1   MASSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSADLCE 60

Query: 61  RQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPT 120
           RQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPT
Sbjct: 61  RQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPT 120

Query: 121 LPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMFANA 180
           LPLMVDIGCG GRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMFANA
Sbjct: 121 LPLMVDIGCGSGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMFANA 180

Query: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240
           TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD
Sbjct: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240

Query: 241 VLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGANIY 300
           VLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGANIY
Sbjct: 241 VLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGANIY 300

Query: 301 RRMYQKYN 309
           RRMYQKYN
Sbjct: 301 RRMYQKYN 308

BLAST of Bhi01G000051 vs. NCBI nr
Match: XP_023527130.1 (uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 561.6 bits (1446), Expect = 4.2e-156
Identity = 278/310 (89.68%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAKA---AAPSRVATANKTLSLFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LR IAAAKA   AAPS VATAN+TLSL LKRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAVAAPSSVATANRTLSLLLKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMF 180
           DPTLPLMVDIG G GRFLLWLAK+H DSRNFLGLEIR+KLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGA 300
           QSDVL+VAVDMRL FDS  DVLKH+D LDQSLLCD+DGWLL NPMGIRTEREIHAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Bhi01G000051 vs. NCBI nr
Match: KAG7018929.1 (trmB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 558.5 bits (1438), Expect = 3.5e-155
Identity = 275/310 (88.71%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAK---AAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LR IAAAK   AAAPS VATAN+TLSL +KRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMF 180
           DPTLPLMVDIG G GRFLLWLAK+H DSRNFLGLEIR+KLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGA 300
           QSDVL+VAVDMRL FDS  DVLKH+D LDQSLLCD++GWLL NPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Bhi01G000051 vs. NCBI nr
Match: XP_022980361.1 (uncharacterized protein LOC111479752 [Cucurbita maxima])

HSP 1 Score: 558.1 bits (1437), Expect = 4.6e-155
Identity = 276/310 (89.03%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAK---AAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LR IAAAK   AAAPS VATAN+TLSL +KRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLHSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMF 180
           DPTLPLMVDIG G GRFLLWLAK+H DSRNFLGLEIR+KLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSL PGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQVFI 240

Query: 241 QSDVLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGA 300
           QSDVL+VAVDMRL FDS  +VLKH+D LDQSLLCD+DGWLLNNPMGIRTEREIHAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Bhi01G000051 vs. NCBI nr
Match: XP_022924717.1 (uncharacterized protein LOC111432133 [Cucurbita moschata])

HSP 1 Score: 555.8 bits (1431), Expect = 2.3e-154
Identity = 275/310 (88.71%), Postives = 289/310 (93.23%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAK---AAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LR IAAAK   AAAPS VATAN+TLSL +KRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMF 180
           DPTLPLMVDIG G GRFLLWLAK+H DSRNFLGLEIR+KLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGA 300
           QSDVL+VAVDMRL FDS  +VLKH+D  DQSLLCD+DGWLL NPMGIRTEREIHAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Bhi01G000051 vs. ExPASy TrEMBL
Match: A0A6J1ITE1 (uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.2e-155
Identity = 276/310 (89.03%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAK---AAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LR IAAAK   AAAPS VATAN+TLSL +KRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLHSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMF 180
           DPTLPLMVDIG G GRFLLWLAK+H DSRNFLGLEIR+KLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSL PGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQVFI 240

Query: 241 QSDVLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGA 300
           QSDVL+VAVDMRL FDS  +VLKH+D LDQSLLCD+DGWLLNNPMGIRTEREIHAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Bhi01G000051 vs. ExPASy TrEMBL
Match: A0A6J1E9S1 (uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC111432133 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.1e-154
Identity = 275/310 (88.71%), Postives = 289/310 (93.23%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAK---AAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LR IAAAK   AAAPS VATAN+TLSL +KRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMF 180
           DPTLPLMVDIG G GRFLLWLAK+H DSRNFLGLEIR+KLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGA 300
           QSDVL+VAVDMRL FDS  +VLKH+D  DQSLLCD+DGWLL NPMGIRTEREIHAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Bhi01G000051 vs. ExPASy TrEMBL
Match: A0A5A7U1F8 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003710 PE=4 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.1e-152
Identity = 270/306 (88.24%), Postives = 284/306 (92.81%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSADLCE 60
           MASSLCLHLQTCSHLR + AAK AAPS VATANK LS   KRPFL NF LPSGSSADLCE
Sbjct: 1   MASSLCLHLQTCSHLRCLGAAKPAAPSVVATANKILS---KRPFLRNFSLPSGSSADLCE 60

Query: 61  RQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPT 120
           RQPLRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+DPT
Sbjct: 61  RQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPT 120

Query: 121 LPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMFANA 180
           LPLMVDIG G GRFLLWLAKRH +SRNFLGLEIR+KLVKRA  WVKELGFSNVHFMFANA
Sbjct: 121 LPLMVDIGSGSGRFLLWLAKRHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMFANA 180

Query: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240
           TISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD
Sbjct: 181 TISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240

Query: 241 VLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGANIY 300
           VL+VA DMRL FDS+ D+L+HIDK+D+S+LCD+DGWLLNNPMGIRTEREIHAESEGANIY
Sbjct: 241 VLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGANIY 300

Query: 301 RRMYQK 307
           RRMY K
Sbjct: 301 RRMYSK 303

BLAST of Bhi01G000051 vs. ExPASy TrEMBL
Match: A0A1S3AVT2 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 PE=4 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 8.8e-152
Identity = 270/306 (88.24%), Postives = 284/306 (92.81%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSADLCE 60
           MASSLCLHLQTCSHLR +AAAK AAPS VATANK LS   KRPFL NF LPSGSSADLCE
Sbjct: 1   MASSLCLHLQTCSHLRCLAAAKPAAPSVVATANKILS---KRPFLRNFSLPSGSSADLCE 60

Query: 61  RQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPT 120
           RQPLRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+DPT
Sbjct: 61  RQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPT 120

Query: 121 LPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMFANA 180
           LPLMVDIG G GRFLLWLAK H +SRNFLGLEIR+KLVKRA  WVKELGFSNVHFMFANA
Sbjct: 121 LPLMVDIGSGSGRFLLWLAKSHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMFANA 180

Query: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240
           TISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD
Sbjct: 181 TISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240

Query: 241 VLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGANIY 300
           VL+VA DMRL FDS+ D+L+HIDK+D+S+LCD+DGWLLNNPMGIRTEREIHAESEGANIY
Sbjct: 241 VLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGANIY 300

Query: 301 RRMYQK 307
           RRMY K
Sbjct: 301 RRMYSK 303

BLAST of Bhi01G000051 vs. ExPASy TrEMBL
Match: A0A0A0L947 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126850 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 3.7e-150
Identity = 270/306 (88.24%), Postives = 284/306 (92.81%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSADLCE 60
           MASSLCLHLQ  SHLR IAAAK AAPS VATANK LS   KRPFL NF LPSGSSADLCE
Sbjct: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILS---KRPFLRNFSLPSGSSADLCE 60

Query: 61  RQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPT 120
           RQPLRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+DPT
Sbjct: 61  RQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPT 120

Query: 121 LPLMVDIGCGHGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMFANA 180
           LPLMVDIG G GRFLLWLAKRH +SRNFLGLEIR+KLVKRA IWVKELGFSNVHFMFANA
Sbjct: 121 LPLMVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNVHFMFANA 180

Query: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240
           TISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD
Sbjct: 181 TISFKQIISSYPGPLILVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240

Query: 241 VLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGANIY 300
           VL+VA DMRL FDS+A +L+HI+K+D+S+LCD+DGWLLNNPMGIRTEREIHAESEGANIY
Sbjct: 241 VLEVAADMRLWFDSEAHILEHINKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGANIY 300

Query: 301 RRMYQK 307
           RRMY K
Sbjct: 301 RRMYSK 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G17660.14.1e-10160.00tRNA (guanine-N-7) methyltransferase [more]
AT5G24840.11.8e-1129.14tRNA (guanine-N-7) methyltransferase [more]
Match NameE-valueIdentityDescription
Q2JJQ01.1e-4246.26tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-... [more]
Q2JRH15.5e-4246.45tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX... [more]
P731612.1e-4143.32tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / K... [more]
P725461.5e-3942.13tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 79... [more]
Q5N2X51.5e-3942.13tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 /... [more]
Match NameE-valueIdentityDescription
XP_038879912.15.2e-17599.68tRNA (guanine-N(7)-)-methyltransferase [Benincasa hispida][more]
XP_023527130.14.2e-15689.68uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo][more]
KAG7018929.13.5e-15588.71trmB [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022980361.14.6e-15589.03uncharacterized protein LOC111479752 [Cucurbita maxima][more]
XP_022924717.12.3e-15488.71uncharacterized protein LOC111432133 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1ITE12.2e-15589.03uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752... [more]
A0A6J1E9S11.1e-15488.71uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC1114321... [more]
A0A5A7U1F85.1e-15288.24tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3AVT28.8e-15288.24tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 P... [more]
A0A0A0L9473.7e-15088.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126850 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typeTIGRFAMTIGR00091TIGR00091coord: 110..306
e-value: 1.1E-33
score: 114.3
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePFAMPF02390Methyltransf_4coord: 122..301
e-value: 2.7E-33
score: 114.9
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePANTHERPTHR234173-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASEcoord: 53..306
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePROSITEPS51625SAM_MT_TRMBcoord: 80..306
score: 52.389847
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 91..308
e-value: 2.9E-55
score: 188.7
NoneNo IPR availablePANTHERPTHR23417:SF21TRNA (GUANINE-N(7)-)-METHYLTRANSFERASEcoord: 53..306
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 125..238
e-value: 2.28091E-5
score: 40.8763
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 122..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000051Bhi01M000051mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0106004 tRNA (guanine-N7)-methylation
biological_process GO:0006400 tRNA modification
cellular_component GO:0043527 tRNA methyltransferase complex
molecular_function GO:0008176 tRNA (guanine-N7-)-methyltransferase activity