MELO3C009818 (gene) Melon (DHL92) v3.5.1

NameMELO3C009818
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionCalcium-binding family protein
Locationchr4 : 27779886 .. 27780673 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTTGTCCTCAAAACAAATTTCCAATTCCAAAACCCCAAATCACATTCAAATTTTCATTTCCCCCAATATGAAGCTTCTCAAATCCTTCAACCCCAACCGTCTCTTCTCTTCCAAGTCCCACCGCTCCAAACTCTCCCGATCTCAACCTCCTTCTTTCGGCTCCGCCTCCTCCTCCTCCTCCTCCACCTCCTCCGCCGGAACCCCCACCAGCGTCTTGCCGTCCCTCGACTGGGCCGATTTCTCCGACCCCACTCTCTCTCGTAAAGAACTGAAGGCTCTACTCGGCCTAATCGGAGCCGAACCGCCGAGTGAGGAAGAGATTAAGATCATGATGGGCGAAATGGACCGGGTTGGACCGGCTTGCCACTCCGAGTTACGTGATACGTTTGAGATATTCGACGCCGACCACGACGGGAGAATTACGGCGGAGGAGCTCTTTAGTGTGTTCACGGCGATGGGAGACGAGGGGTGCACGTTAGAGGATTGCCGGCGTATGATAGCAGGTGTAGACAAAAATGGAGATGGGTTCGTCTGCTTCGACGACTTTGCGCGTATGATGGACTGCCAGAGATAAATCCAATCAACACCTTTCATTTTTTTTTCTTTTAAACTCTTTTACTTATTAATTATAATAATAATAATTTTGAAAAAGAAAAGGGAAAAAAAAAAAGAAGAAGAAGAAGAAGGGATCTTTGTGTTTATGTTTGTTGATGCAAAGTATATAATTAATTAAAAACGGCAGTAAGTCGTGGGATCGGTCATTCGTCAAATTCATCCCTTTCTCCAA

mRNA sequence

ACTTGTCCTCAAAACAAATTTCCAATTCCAAAACCCCAAATCACATTCAAATTTTCATTTCCCCCAATATGAAGCTTCTCAAATCCTTCAACCCCAACCGTCTCTTCTCTTCCAAGTCCCACCGCTCCAAACTCTCCCGATCTCAACCTCCTTCTTTCGGCTCCGCCTCCTCCTCCTCCTCCTCCACCTCCTCCGCCGGAACCCCCACCAGCGTCTTGCCGTCCCTCGACTGGGCCGATTTCTCCGACCCCACTCTCTCTCGTAAAGAACTGAAGGCTCTACTCGGCCTAATCGGAGCCGAACCGCCGAGTGAGGAAGAGATTAAGATCATGATGGGCGAAATGGACCGGGTTGGACCGGCTTGCCACTCCGAGTTACGTGATACGTTTGAGATATTCGACGCCGACCACGACGGGAGAATTACGGCGGAGGAGCTCTTTAGTGTGTTCACGGCGATGGGAGACGAGGGGTGCACGTTAGAGGATTGCCGGCGTATGATAGCAGGTGTAGACAAAAATGGAGATGGGTTCGTCTGCTTCGACGACTTTGCGCGTATGATGGACTGCCAGAGATAAATCCAATCAACACCTTTCATTTTTTTTTCTTTTAAACTCTTTTACTTATTAATTATAATAATAATAATTTTGAAAAAGAAAAGGGAAAAAAAAAAAGAAGAAGAAGAAGAAGGGATCTTTGTGTTTATGTTTGTTGATGCAAAGTATATAATTAATTAAAAACGGCAGTAAGTCGTGGGATCGGTCATTCGTCAAATTCATCCCTTTCTCCAA

Coding sequence (CDS)

ATGAAGCTTCTCAAATCCTTCAACCCCAACCGTCTCTTCTCTTCCAAGTCCCACCGCTCCAAACTCTCCCGATCTCAACCTCCTTCTTTCGGCTCCGCCTCCTCCTCCTCCTCCTCCACCTCCTCCGCCGGAACCCCCACCAGCGTCTTGCCGTCCCTCGACTGGGCCGATTTCTCCGACCCCACTCTCTCTCGTAAAGAACTGAAGGCTCTACTCGGCCTAATCGGAGCCGAACCGCCGAGTGAGGAAGAGATTAAGATCATGATGGGCGAAATGGACCGGGTTGGACCGGCTTGCCACTCCGAGTTACGTGATACGTTTGAGATATTCGACGCCGACCACGACGGGAGAATTACGGCGGAGGAGCTCTTTAGTGTGTTCACGGCGATGGGAGACGAGGGGTGCACGTTAGAGGATTGCCGGCGTATGATAGCAGGTGTAGACAAAAATGGAGATGGGTTCGTCTGCTTCGACGACTTTGCGCGTATGATGGACTGCCAGAGATAA

Protein sequence

MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR*
BLAST of MELO3C009818 vs. Swiss-Prot
Match: CML35_ARATH (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana GN=CML35 PE=2 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 1.3e-34
Identity = 95/209 (45.45%), Postives = 115/209 (55.02%), Query Frame = 1

Query: 1   MKLLKSFN---PNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAG--------TPTSV 60
           MKL  S N   P RLF +KS ++ +SRS+P SF S +SSSSS  S G        TP SV
Sbjct: 1   MKLAASLNRLSPKRLFRTKS-KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISV 60

Query: 61  LPSLDWADF--------------SDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRV 120
           LP  +  DF               D  +SR +L AL+  +  EPPS+EE+ +M+ E+D  
Sbjct: 61  LPQ-NSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGG 120

Query: 121 GPACHS--------------------ELRDTFEIFDADHDGRITAEELFSVFTAMGDEGC 165
              C S                    ELR+ FEIFD D +G+I+AEEL  VF  +GDE C
Sbjct: 121 DGGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERC 180

BLAST of MELO3C009818 vs. Swiss-Prot
Match: CML36_ARATH (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana GN=CML36 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.7e-32
Identity = 92/210 (43.81%), Postives = 120/210 (57.14%), Query Frame = 1

Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPP-SFGSASSSSSST--SSAG-------TPTSVL 60
           MKL K   P R F     RS +S+S    SFGSASSSS     +S G       TPTS+L
Sbjct: 1   MKLAKLI-PKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  PSL----DWADF----------SDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVG 120
           P +     + +           +D  +SR +L++LL  +G +P +EEEI +M+ E+D  G
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 -----------------PACHS-ELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLE 169
                            PA  S EL++TFE FDAD DG I+A+EL  VF+ +GDE CTL+
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

BLAST of MELO3C009818 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.0e-14
Identity = 47/124 (37.90%), Postives = 69/124 (55.65%), Query Frame = 1

Query: 60  DPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHS------------------ 119
           D  +S KEL A++  +G E P EEE++  + E+DR G    +                  
Sbjct: 50  DGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 120 -ELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDF 165
             L+D F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + F++F
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169


HSP 2 Score: 48.1 bits (113), Expect = 1.1e-04
Identity = 21/62 (33.87%), Postives = 38/62 (61.29%), Query Frame = 1

Query: 102 ELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFA 161
           EL   F+ FD + DG+I+++EL ++ T++G E    E+  + I  +D+ GDG++ F++F 
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHE-VPEEELEKAITEIDRKGDGYINFEEFV 96

Query: 162 RM 164
            +
Sbjct: 97  EL 97

BLAST of MELO3C009818 vs. Swiss-Prot
Match: CML16_ORYSJ (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica GN=CML16 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 9.7e-14
Identity = 54/128 (42.19%), Postives = 67/128 (52.34%), Query Frame = 1

Query: 60  DPTLSRKELKALLGLIGAEPPSEE----EIKIMMGEMDRV------------------GP 119
           D  +S  EL A+   I A PPSE     E+  MM E+D                    G 
Sbjct: 40  DGRISPSELAAVTRAI-APPPSESAGGREVAAMMNELDTDRDGFVDLGEFAAFHGRGRGD 99

Query: 120 ACH-SELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVC 165
           A H +ELR  F+++D D DGRITA EL  V   +G EGC+ E+C RMIA VD +GDG V 
Sbjct: 100 AEHEAELRAAFDVYDVDGDGRITAAELGKVLGRIG-EGCSAEECERMIASVDVDGDGCVG 159


HSP 2 Score: 37.0 bits (84), Expect = 2.5e-01
Identity = 23/64 (35.94%), Postives = 31/64 (48.44%), Query Frame = 1

Query: 101 SELRDTFEIFDADHDGRITAEELFSVFTAMG---DEGCTLEDCRRMIAGVDKNGDGFVCF 160
           +E+   F  FDAD DGRI+  EL +V  A+     E     +   M+  +D + DGFV  
Sbjct: 26  AEIERVFTRFDADGDGRISPSELAAVTRAIAPPPSESAGGREVAAMMNELDTDRDGFVDL 85

Query: 161 DDFA 162
            +FA
Sbjct: 86  GEFA 89

BLAST of MELO3C009818 vs. Swiss-Prot
Match: CML1_ARATH (Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.2e-13
Identity = 57/172 (33.14%), Postives = 79/172 (45.93%), Query Frame = 1

Query: 9   PNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSDPTLSRKEL 68
           P+R+FS     S LS   P  F        S  S      V    D     D  +S+ E 
Sbjct: 22  PSRMFSRDRQSSGLSSPGPGGF--------SQPSVNEMRRVFSRFDLD--KDGKISQTEY 81

Query: 69  KALLGLIGAEPPSEEEIKIMMG-EMD---------------RVGPACHSELRDTFEIFDA 128
           K +L  +G E   E+  KI    ++D               R G    S++R++F  FD 
Sbjct: 82  KVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSDIRNSFWTFDL 141

Query: 129 DHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMM 165
           + DG+I+AEE+ SV   +G E C+LEDC RM+  VD +GDG V  ++F +MM
Sbjct: 142 NGDGKISAEEVMSVLWKLG-ERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

BLAST of MELO3C009818 vs. TrEMBL
Match: A0A0A0LFY7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G823060 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 3.3e-85
Identity = 159/168 (94.64%), Postives = 163/168 (97.02%), Query Frame = 1

Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSFGSASSSSSS S+AGTPTSVLPSLDW+DFSD
Sbjct: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSS-SAAGTPTSVLPSLDWSDFSD 60

Query: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120
           PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGP CHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 169
           EELFSVF AMGD+GCTLEDC+RMIAGVDKNGDGFVCFDDF RMMDCQR
Sbjct: 121 EELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

BLAST of MELO3C009818 vs. TrEMBL
Match: M5WA96_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010940mg PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.2e-47
Identity = 120/229 (52.40%), Postives = 145/229 (63.32%), Query Frame = 1

Query: 1   MKLLK----SFNPNRLFSSKSHRSKLSR--SQPPSF--GSASSSSSSTSSA--------- 60
           MKL+K    S +P RLF SK  RS +SR  S P SF  G+ASSSSSS+SS+         
Sbjct: 1   MKLIKISSKSLSPKRLFRSKKDRSAVSRRSSDPLSFESGTASSSSSSSSSSDSLHKPGTG 60

Query: 61  ------GTPTSVL--PSLDWADFS------------------DPTLSRKELKALLGLIGA 120
                 GTPTSVL   S DW+DFS                  D  +SRKEL+ALL  +GA
Sbjct: 61  AEAGGIGTPTSVLLERSGDWSDFSTDLQLDMAQAFKLIDRDNDGVVSRKELEALLSRLGA 120

Query: 121 EPPSEEEIKIMMGEMDR------------------VGPACHSELRDTFEIFDADHDGRIT 169
           +PPS+EE+ +M+ E+DR                   GPA  SELRD FE+FD+DHDG+I+
Sbjct: 121 DPPSQEEVMLMLSEVDREGNGSISLEALLSRVGPVCGPAADSELRDAFEVFDSDHDGKIS 180

BLAST of MELO3C009818 vs. TrEMBL
Match: A0A067D4X5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 3.3e-45
Identity = 107/212 (50.47%), Postives = 135/212 (63.68%), Query Frame = 1

Query: 1   MKLLKSFNPNRLFSSKSH--RSKLSRSQPPSFGSASSSSSSTSS--------AGTPTSVL 60
           MKL++  +P RLF SKS   R+ +SRS P SF S+S+S SST +         GTPTSVL
Sbjct: 1   MKLIEKLSPKRLFRSKSKKDRTSVSRSDPSSFSSSSTSDSSTHNKLGSAPDRVGTPTSVL 60

Query: 61  PSL--DWADFS----------------------DPTLSRKELKALLGLIGAEPPSEEEIK 120
             +  DW+D S                      D  + R EL+ALL  +GA+PP++EE+K
Sbjct: 61  HEISGDWSDMSADISLDMNYELVQACKLLDRDNDGVVLRSELEALLIRLGADPPTQEEVK 120

Query: 121 IMMGEMDRVG----------PACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCT 169
            M+ E+DR G          PAC  EL++TF+ FDADHDG+ITAEELF VFT +GDE CT
Sbjct: 121 SMLSEVDREGDGRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGVFTKLGDELCT 180

BLAST of MELO3C009818 vs. TrEMBL
Match: V4SC13_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005842mg PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.7e-44
Identity = 107/221 (48.42%), Postives = 135/221 (61.09%), Query Frame = 1

Query: 1   MKLLKSFNPNRLFSSKSH--RSKLSRSQPPSFGSASSSSSSTSS--------AGTPTSVL 60
           MKL++  +P RLF SKS   R+ +SRS P SF S+S+S SST +         GTPTSVL
Sbjct: 1   MKLIEKLSPKRLFRSKSKKDRTSVSRSDPSSFSSSSTSDSSTHNKLGSAPDRVGTPTSVL 60

Query: 61  PSL--DWADFS----------------------DPTLSRKELKALLGLIGAEPPSEEEIK 120
             +  DW+D S                      D  + R EL+ALL  +GA+PP++EE+K
Sbjct: 61  HEISGDWSDMSADISLDMNYELVQACKLLDRDNDGVVLRSELEALLIRLGADPPTQEEVK 120

Query: 121 IMMGEMDRVG-------------------PACHSELRDTFEIFDADHDGRITAEELFSVF 169
            M+ E+DR G                   PAC  EL++TF+ FDADHDG+ITAEELF VF
Sbjct: 121 SMLSEVDREGDGYIPLEALISRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGVF 180

BLAST of MELO3C009818 vs. TrEMBL
Match: A0A067DGT9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.7e-44
Identity = 107/221 (48.42%), Postives = 135/221 (61.09%), Query Frame = 1

Query: 1   MKLLKSFNPNRLFSSKSH--RSKLSRSQPPSFGSASSSSSSTSS--------AGTPTSVL 60
           MKL++  +P RLF SKS   R+ +SRS P SF S+S+S SST +         GTPTSVL
Sbjct: 1   MKLIEKLSPKRLFRSKSKKDRTSVSRSDPSSFSSSSTSDSSTHNKLGSAPDRVGTPTSVL 60

Query: 61  PSL--DWADFS----------------------DPTLSRKELKALLGLIGAEPPSEEEIK 120
             +  DW+D S                      D  + R EL+ALL  +GA+PP++EE+K
Sbjct: 61  HEISGDWSDMSADISLDMNYELVQACKLLDRDNDGVVLRSELEALLIRLGADPPTQEEVK 120

Query: 121 IMMGEMDRVG-------------------PACHSELRDTFEIFDADHDGRITAEELFSVF 169
            M+ E+DR G                   PAC  EL++TF+ FDADHDG+ITAEELF VF
Sbjct: 121 SMLSEVDREGDGYIPLEALISRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGVF 180

BLAST of MELO3C009818 vs. TAIR10
Match: AT2G41410.1 (AT2G41410.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 147.5 bits (371), Expect = 7.4e-36
Identity = 95/209 (45.45%), Postives = 115/209 (55.02%), Query Frame = 1

Query: 1   MKLLKSFN---PNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAG--------TPTSV 60
           MKL  S N   P RLF +KS ++ +SRS+P SF S +SSSSS  S G        TP SV
Sbjct: 1   MKLAASLNRLSPKRLFRTKS-KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISV 60

Query: 61  LPSLDWADF--------------SDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRV 120
           LP  +  DF               D  +SR +L AL+  +  EPPS+EE+ +M+ E+D  
Sbjct: 61  LPQ-NSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGG 120

Query: 121 GPACHS--------------------ELRDTFEIFDADHDGRITAEELFSVFTAMGDEGC 165
              C S                    ELR+ FEIFD D +G+I+AEEL  VF  +GDE C
Sbjct: 121 DGGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERC 180

BLAST of MELO3C009818 vs. TAIR10
Match: AT3G10190.1 (AT3G10190.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 139.8 bits (351), Expect = 1.5e-33
Identity = 92/210 (43.81%), Postives = 120/210 (57.14%), Query Frame = 1

Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPP-SFGSASSSSSST--SSAG-------TPTSVL 60
           MKL K   P R F     RS +S+S    SFGSASSSS     +S G       TPTS+L
Sbjct: 1   MKLAKLI-PKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  PSL----DWADF----------SDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVG 120
           P +     + +           +D  +SR +L++LL  +G +P +EEEI +M+ E+D  G
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 -----------------PACHS-ELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLE 169
                            PA  S EL++TFE FDAD DG I+A+EL  VF+ +GDE CTL+
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

BLAST of MELO3C009818 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 80.5 bits (197), Expect = 1.1e-15
Identity = 47/124 (37.90%), Postives = 69/124 (55.65%), Query Frame = 1

Query: 60  DPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHS------------------ 119
           D  +S KEL A++  +G E P EEE++  + E+DR G    +                  
Sbjct: 50  DGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 120 -ELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDF 165
             L+D F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + F++F
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169


HSP 2 Score: 48.1 bits (113), Expect = 6.1e-06
Identity = 21/62 (33.87%), Postives = 38/62 (61.29%), Query Frame = 1

Query: 102 ELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFA 161
           EL   F+ FD + DG+I+++EL ++ T++G E    E+  + I  +D+ GDG++ F++F 
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHE-VPEEELEKAITEIDRKGDGYINFEEFV 96

Query: 162 RM 164
            +
Sbjct: 97  EL 97

BLAST of MELO3C009818 vs. TAIR10
Match: AT2G15680.1 (AT2G15680.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 75.1 bits (183), Expect = 4.6e-14
Identity = 57/172 (33.14%), Postives = 79/172 (45.93%), Query Frame = 1

Query: 9   PNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSDPTLSRKEL 68
           P+R+FS     S LS   P  F        S  S      V    D     D  +S+ E 
Sbjct: 22  PSRMFSRDRQSSGLSSPGPGGF--------SQPSVNEMRRVFSRFDLD--KDGKISQTEY 81

Query: 69  KALLGLIGAEPPSEEEIKIMMG-EMD---------------RVGPACHSELRDTFEIFDA 128
           K +L  +G E   E+  KI    ++D               R G    S++R++F  FD 
Sbjct: 82  KVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSDIRNSFWTFDL 141

Query: 129 DHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMM 165
           + DG+I+AEE+ SV   +G E C+LEDC RM+  VD +GDG V  ++F +MM
Sbjct: 142 NGDGKISAEEVMSVLWKLG-ERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

BLAST of MELO3C009818 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 73.2 bits (178), Expect = 1.8e-13
Identity = 44/127 (34.65%), Postives = 68/127 (53.54%), Query Frame = 1

Query: 59  SDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGP--------------------- 118
           +D  +S  ELK ++G +     S+EE K MM E D  G                      
Sbjct: 27  NDGKISIDELKDVIGALSPNA-SQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSNN 86

Query: 119 ACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCF 165
           +   +L++ F+++D D +GRI+A EL SV   +G++ C+++DC+RMI  VD +GDG V F
Sbjct: 87  SAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK-CSIQDCQRMINKVDSDGDGCVDF 146

BLAST of MELO3C009818 vs. NCBI nr
Match: gi|659085286|ref|XP_008443339.1| (PREDICTED: probable calcium-binding protein CML35 [Cucumis melo])

HSP 1 Score: 341.3 bits (874), Expect = 9.8e-91
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 1

Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 60
           MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD
Sbjct: 66  MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 125

Query: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120
           PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA
Sbjct: 126 PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 185

Query: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 169
           EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR
Sbjct: 186 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 233

BLAST of MELO3C009818 vs. NCBI nr
Match: gi|778685391|ref|XP_004136671.2| (PREDICTED: probable calcium-binding protein CML35 [Cucumis sativus])

HSP 1 Score: 322.4 bits (825), Expect = 4.7e-85
Identity = 159/168 (94.64%), Postives = 163/168 (97.02%), Query Frame = 1

Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSFGSASSSSSS S+AGTPTSVLPSLDW+DFSD
Sbjct: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSS-SAAGTPTSVLPSLDWSDFSD 60

Query: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120
           PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGP CHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 169
           EELFSVF AMGD+GCTLEDC+RMIAGVDKNGDGFVCFDDF RMMDCQR
Sbjct: 121 EELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

BLAST of MELO3C009818 vs. NCBI nr
Match: gi|645271060|ref|XP_008240735.1| (PREDICTED: probable calcium-binding protein CML36 [Prunus mume])

HSP 1 Score: 203.0 bits (515), Expect = 4.2e-49
Identity = 118/229 (51.53%), Postives = 145/229 (63.32%), Query Frame = 1

Query: 1   MKLLK----SFNPNRLFSSKSHRSKLSR--SQPPSFGSASSSSSSTSSAG---------- 60
           MKL+K    S +P  LF SK  RS +SR  S PPSFGS ++SSSS+SS+           
Sbjct: 1   MKLIKISSKSLSPKHLFRSKKDRSAVSRRSSDPPSFGSGTASSSSSSSSSSDSLHKPGTG 60

Query: 61  -------TPTSVLP--SLDWADFS------------------DPTLSRKELKALLGLIGA 120
                  TPTSVLP  S DW+DFS                  D  +SRKEL+ALL  +GA
Sbjct: 61  AEAGGIRTPTSVLPERSGDWSDFSTDLQLDMAQAFKLIDRDNDGVVSRKELEALLSRLGA 120

Query: 121 EPPSEEEIKIMMGEMDR------------------VGPACHSELRDTFEIFDADHDGRIT 169
           +PPS+EE+ +M+ E+DR                   GPA  SELRD F++FD+DHDG+I+
Sbjct: 121 DPPSQEEVMLMLSEVDREGNGSITLDALLNRVGPVCGPAADSELRDAFKVFDSDHDGKIS 180

BLAST of MELO3C009818 vs. NCBI nr
Match: gi|470135628|ref|XP_004303614.1| (PREDICTED: probable calcium-binding protein CML36 [Fragaria vesca subsp. vesca])

HSP 1 Score: 202.2 bits (513), Expect = 7.1e-49
Identity = 117/211 (55.45%), Postives = 140/211 (66.35%), Query Frame = 1

Query: 1   MKLLK--SFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSS-------AGTPTSVLP 60
           MKL+K  S +P RLF SK  RS +SRS PPSFGS ++SSSS+ S       A TPTSVLP
Sbjct: 1   MKLMKINSLSPKRLFRSKKDRSAVSRSDPPSFGSGTASSSSSDSLHKPGGPAATPTSVLP 60

Query: 61  SLDWADFS---------------DPTLSRKELKALLG-LIGAEPPSEEEIKIMMGEMD-- 120
             DW++FS               D  +SR EL+ALL  L+GAEPP++EE+ +M+ E+D  
Sbjct: 61  G-DWSEFSSVDMAQAFRLIDRDNDGVVSRTELEALLKRLLGAEPPTQEEVAMMLSEVDGD 120

Query: 121 ------------RVGPACH----SELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTL 169
                       R GP C     SELR+ FE+FD DHDG+I+AEEL SVFTA+GDE CTL
Sbjct: 121 GDGCIPLEALLHRFGPGCEPAADSELREAFEVFDTDHDGKISAEELLSVFTAIGDERCTL 180

BLAST of MELO3C009818 vs. NCBI nr
Match: gi|595806938|ref|XP_007202521.1| (hypothetical protein PRUPE_ppa010940mg [Prunus persica])

HSP 1 Score: 197.6 bits (501), Expect = 1.8e-47
Identity = 120/229 (52.40%), Postives = 145/229 (63.32%), Query Frame = 1

Query: 1   MKLLK----SFNPNRLFSSKSHRSKLSR--SQPPSF--GSASSSSSSTSSA--------- 60
           MKL+K    S +P RLF SK  RS +SR  S P SF  G+ASSSSSS+SS+         
Sbjct: 1   MKLIKISSKSLSPKRLFRSKKDRSAVSRRSSDPLSFESGTASSSSSSSSSSDSLHKPGTG 60

Query: 61  ------GTPTSVL--PSLDWADFS------------------DPTLSRKELKALLGLIGA 120
                 GTPTSVL   S DW+DFS                  D  +SRKEL+ALL  +GA
Sbjct: 61  AEAGGIGTPTSVLLERSGDWSDFSTDLQLDMAQAFKLIDRDNDGVVSRKELEALLSRLGA 120

Query: 121 EPPSEEEIKIMMGEMDR------------------VGPACHSELRDTFEIFDADHDGRIT 169
           +PPS+EE+ +M+ E+DR                   GPA  SELRD FE+FD+DHDG+I+
Sbjct: 121 DPPSQEEVMLMLSEVDREGNGSISLEALLSRVGPVCGPAADSELRDAFEVFDSDHDGKIS 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CML35_ARATH1.3e-3445.45Probable calcium-binding protein CML35 OS=Arabidopsis thaliana GN=CML35 PE=2 SV=... [more]
CML36_ARATH2.7e-3243.81Probable calcium-binding protein CML36 OS=Arabidopsis thaliana GN=CML36 PE=2 SV=... [more]
CML25_ARATH2.0e-1437.90Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
CML16_ORYSJ9.7e-1442.19Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica GN=CML16 ... [more]
CML1_ARATH8.2e-1333.14Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LFY7_CUCSA3.3e-8594.64Uncharacterized protein OS=Cucumis sativus GN=Csa_3G823060 PE=4 SV=1[more]
M5WA96_PRUPE1.2e-4752.40Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010940mg PE=4 SV=1[more]
A0A067D4X5_CITSI3.3e-4550.47Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1[more]
V4SC13_9ROSI3.7e-4448.42Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005842mg PE=4 SV=1[more]
A0A067DGT9_CITSI3.7e-4448.42Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G41410.17.4e-3645.45 Calcium-binding EF-hand family protein[more]
AT3G10190.11.5e-3343.81 Calcium-binding EF-hand family protein[more]
AT1G24620.11.1e-1537.90 EF hand calcium-binding protein family[more]
AT2G15680.14.6e-1433.14 Calcium-binding EF-hand family protein[more]
AT1G66400.11.8e-1334.65 calmodulin like 23[more]
Match NameE-valueIdentityDescription
gi|659085286|ref|XP_008443339.1|9.8e-91100.00PREDICTED: probable calcium-binding protein CML35 [Cucumis melo][more]
gi|778685391|ref|XP_004136671.2|4.7e-8594.64PREDICTED: probable calcium-binding protein CML35 [Cucumis sativus][more]
gi|645271060|ref|XP_008240735.1|4.2e-4951.53PREDICTED: probable calcium-binding protein CML36 [Prunus mume][more]
gi|470135628|ref|XP_004303614.1|7.1e-4955.45PREDICTED: probable calcium-binding protein CML36 [Fragaria vesca subsp. vesca][more]
gi|595806938|ref|XP_007202521.1|1.8e-4752.40hypothetical protein PRUPE_ppa010940mg [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU45776melon EST collection version 4.0transcribed_cluster
MU61458melon EST collection version 4.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C009818T1MELO3C009818T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU45776MU45776transcribed_cluster
MU61458MU61458transcribed_cluster


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 102..164
score: 1.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 102..130
score: 2.7E-5coord: 139..167
score: 0.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 135..168
score: 11.584coord: 98..133
score: 13.091coord: 62..78
score: 5
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 60..164
score: 9.9
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 56..164
score: 3.71
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 111..123
score: -coord: 148..160
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 63..164
score: 1.1
NoneNo IPR availablePANTHERPTHR10891:SF590SUBFAMILY NOT NAMEDcoord: 63..164
score: 1.1