CmaCh14G005570 (gene) Cucurbita maxima (Rimu)

NameCmaCh14G005570
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionEF hand calcium-binding family protein
LocationCma_Chr14 : 2777161 .. 2777682 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTCCCCAAAATCACCGCCAAATCCTTCTCCCCCGGCCGTCTTTTCCCTTCCAAAAAGCACCGCACCAGCCTCTCTAAATCCGAGCCCCCTTCCTTCGGCTCCGCCTCATTCTCCTCCCCGACCAGCGTCTTGCCGCCTCACCCGCACCCCCACCTCTCCGGCGAATGGTCCGACTTCTCCGACACCCACATCGACTATCGCAAAGAACTGGAGGCTCTACTAGGCCTCATCGGAGCCGAACCGCCGAGCGAAGAGGAGCTCAAGATGATGATGAGCCAAGTAGACAAAGTCGGCCCGGCTTGCCACTCCGAACTGCGAGATACGTTTGAAATCTTCGACGCCGACCACGACGGGAGAATCACGGCGGAGGAGCTTTTTAGTGTGTTCTCCGCCATGGGAGATGAGCAGTGCACGTTGGAGGATTGCCGGCGTATGATAGCCGGAGTGGACAAAAACGGGGATGGGTTCGTGTGCTTCGAGGACTTTGCGGTTATGATGGAGTGCCAGAGATGTTGA

mRNA sequence

ATGAAGCTCCCCAAAATCACCGCCAAATCCTTCTCCCCCGGCCGTCTTTTCCCTTCCAAAAAGCACCGCACCAGCCTCTCTAAATCCGAGCCCCCTTCCTTCGGCTCCGCCTCATTCTCCTCCCCGACCAGCGTCTTGCCGCCTCACCCGCACCCCCACCTCTCCGGCGAATGGTCCGACTTCTCCGACACCCACATCGACTATCGCAAAGAACTGGAGGCTCTACTAGGCCTCATCGGAGCCGAACCGCCGAGCGAAGAGGAGCTCAAGATGATGATGAGCCAAGTAGACAAAGTCGGCCCGGCTTGCCACTCCGAACTGCGAGATACGTTTGAAATCTTCGACGCCGACCACGACGGGAGAATCACGGCGGAGGAGCTTTTTAGTGTGTTCTCCGCCATGGGAGATGAGCAGTGCACGTTGGAGGATTGCCGGCGTATGATAGCCGGAGTGGACAAAAACGGGGATGGGTTCGTGTGCTTCGAGGACTTTGCGGTTATGATGGAGTGCCAGAGATGTTGA

Coding sequence (CDS)

ATGAAGCTCCCCAAAATCACCGCCAAATCCTTCTCCCCCGGCCGTCTTTTCCCTTCCAAAAAGCACCGCACCAGCCTCTCTAAATCCGAGCCCCCTTCCTTCGGCTCCGCCTCATTCTCCTCCCCGACCAGCGTCTTGCCGCCTCACCCGCACCCCCACCTCTCCGGCGAATGGTCCGACTTCTCCGACACCCACATCGACTATCGCAAAGAACTGGAGGCTCTACTAGGCCTCATCGGAGCCGAACCGCCGAGCGAAGAGGAGCTCAAGATGATGATGAGCCAAGTAGACAAAGTCGGCCCGGCTTGCCACTCCGAACTGCGAGATACGTTTGAAATCTTCGACGCCGACCACGACGGGAGAATCACGGCGGAGGAGCTTTTTAGTGTGTTCTCCGCCATGGGAGATGAGCAGTGCACGTTGGAGGATTGCCGGCGTATGATAGCCGGAGTGGACAAAAACGGGGATGGGTTCGTGTGCTTCGAGGACTTTGCGGTTATGATGGAGTGCCAGAGATGTTGA

Protein sequence

MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSSPTSVLPPHPHPHLSGEWSDFSDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC
BLAST of CmaCh14G005570 vs. Swiss-Prot
Match: CML36_ARATH (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana GN=CML36 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.2e-29
Identity = 90/214 (42.06%), Postives = 119/214 (55.61%), Query Frame = 1

Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPP-SFGSASFSS-----------------P 60
           MKL K+      P R F   K R+++SKS    SFGSAS SS                 P
Sbjct: 1   MKLAKLI-----PKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTP 60

Query: 61  TSVLPPHPHPHLSGE------WSDFSDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQV 120
           TS+LP  P P+   E        D  +     R +LE+LL  +G +P +EEE+ +M+ +V
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEV 120

Query: 121 DKVG-----------------PACHS-ELRDTFEIFDADHDGRITAEELFSVFSAMGDEQ 173
           D  G                 PA  S EL++TFE FDAD DG I+A+EL  VFS +GDE+
Sbjct: 121 DCDGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

BLAST of CmaCh14G005570 vs. Swiss-Prot
Match: CML35_ARATH (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana GN=CML35 PE=2 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 4.6e-27
Identity = 81/198 (40.91%), Postives = 106/198 (53.54%), Query Frame = 1

Query: 12  SPGRLFPSKKHRTSLSKSEPPSFGSASFSS----------------PTSVLPPHPHPHLS 71
           SP RLF +K  + S+S+SEP SF S + SS                P SVLP +     +
Sbjct: 11  SPKRLFRTKS-KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISVLPQNSGDFYT 70

Query: 72  G-----EWSDFSDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHS----- 131
                 +  D  D  +  R +L AL+  +  EPPS+EE+ +M+ +VD     C S     
Sbjct: 71  ELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCISLEDLA 130

Query: 132 ---------------ELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAG 169
                          ELR+ FEIFD D +G+I+AEEL  VF  +GDE+CTLE+C RMIA 
Sbjct: 131 SRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEECMRMIAT 190

BLAST of CmaCh14G005570 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.9e-15
Identity = 45/118 (38.14%), Postives = 70/118 (59.32%), Query Frame = 1

Query: 70  KELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHS-------------------ELRDT 129
           KEL A++  +G E P EEEL+  ++++D+ G    +                    L+D 
Sbjct: 56  KELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 115

Query: 130 FEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMM 169
           F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + FE+F +MM
Sbjct: 116 FSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEFKIMM 171

BLAST of CmaCh14G005570 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 60/172 (34.88%), Postives = 82/172 (47.67%), Query Frame = 1

Query: 18  PSKKHRTSLSKSEP-PSFGSASFSSPTSVLPPHPHPHLSGEWSDFSDTHIDYRKELEALL 77
           P+   R S SKS P P    AS     +  P      +  ++    D  I  R EL AL 
Sbjct: 7   PALFRRRSGSKSPPLPQADPASGGGSPAPTPEEEMERVFRKFDANGDGRIS-RSELGALF 66

Query: 78  GLIGAEPPSEEELKMMMSQVDKVGP--------------------ACHSELRDTFEIFDA 137
             +G    +++EL  MM++ D  G                     A   +LR  F +FDA
Sbjct: 67  ESLG-HAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDA 126

Query: 138 DHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMM 169
           D +G I+A EL  V   +G E+ T++ CRRMI GVD+NGDG + FE+F VMM
Sbjct: 127 DGNGTISAAELARVLHGLG-EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

BLAST of CmaCh14G005570 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-12
Identity = 46/129 (35.66%), Postives = 70/129 (54.26%), Query Frame = 1

Query: 63  DTHIDYRKELEALLGLIGAEPP--SEEELKMMMSQVDKVGP------------------- 122
           D + D +  ++ L  +IGA  P  S+EE K MM + D  G                    
Sbjct: 24  DKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSS 83

Query: 123 --ACHSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFV 169
             +   +L++ F+++D D +GRI+A EL SV   +G E+C+++DC+RMI  VD +GDG V
Sbjct: 84  NNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCV 143

BLAST of CmaCh14G005570 vs. TrEMBL
Match: A0A0A0LFY7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G823060 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 3.4e-61
Identity = 124/171 (72.51%), Postives = 141/171 (82.46%), Query Frame = 1

Query: 9   KSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSS-------PTSVLPPHPHPHLSGEWSDF 68
           KS +P RLF SK HR+ LS+S+PPSFGSAS SS       PTSVLP       S +WSDF
Sbjct: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSSAAGTPTSVLP-------SLDWSDF 64

Query: 69  SDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDGR 128
           SD  +  RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGP CHSELRDTFEIFDADHDGR
Sbjct: 65  SDPTLS-RKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGR 124

Query: 129 ITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 173
           ITAEELFSVF+AMGD+ CTLEDC+RMIAGVDKNGDGFVCF+DF  MM+CQR
Sbjct: 125 ITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

BLAST of CmaCh14G005570 vs. TrEMBL
Match: M5WA96_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010940mg PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 8.4e-44
Identity = 114/229 (49.78%), Postives = 142/229 (62.01%), Query Frame = 1

Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSK--SEPPSF--GSASFSSPTSVLPPHPHP---- 60
           MKL KI++KS SP RLF SKK R+++S+  S+P SF  G+AS SS +S      H     
Sbjct: 1   MKLIKISSKSLSPKRLFRSKKDRSAVSRRSSDPLSFESGTASSSSSSSSSSDSLHKPGTG 60

Query: 61  --------------HLSGEWSDFS-DTHIDY----------------RKELEALLGLIGA 120
                           SG+WSDFS D  +D                 RKELEALL  +GA
Sbjct: 61  AEAGGIGTPTSVLLERSGDWSDFSTDLQLDMAQAFKLIDRDNDGVVSRKELEALLSRLGA 120

Query: 121 EPPSEEELKMMMSQVDK------------------VGPACHSELRDTFEIFDADHDGRIT 173
           +PPS+EE+ +M+S+VD+                   GPA  SELRD FE+FD+DHDG+I+
Sbjct: 121 DPPSQEEVMLMLSEVDREGNGSISLEALLSRVGPVCGPAADSELRDAFEVFDSDHDGKIS 180

BLAST of CmaCh14G005570 vs. TrEMBL
Match: A0A067D4X5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 4.3e-40
Identity = 105/213 (49.30%), Postives = 130/213 (61.03%), Query Frame = 1

Query: 9   KSFSPGRLF--PSKKHRTSLSKSEPPSFGSASFS----------------SPTSVLPPHP 68
           +  SP RLF   SKK RTS+S+S+P SF S+S S                +PTSVL    
Sbjct: 5   EKLSPKRLFRSKSKKDRTSVSRSDPSSFSSSSTSDSSTHNKLGSAPDRVGTPTSVLH--- 64

Query: 69  HPHLSGEWSDFS-DTHIDY--------------------RKELEALLGLIGAEPPSEEEL 128
              +SG+WSD S D  +D                     R ELEALL  +GA+PP++EE+
Sbjct: 65  --EISGDWSDMSADISLDMNYELVQACKLLDRDNDGVVLRSELEALLIRLGADPPTQEEV 124

Query: 129 KMMMSQVDKVG----------PACHSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQC 173
           K M+S+VD+ G          PAC  EL++TF+ FDADHDG+ITAEELF VF+ +GDE C
Sbjct: 125 KSMLSEVDREGDGRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGVFTKLGDELC 184

BLAST of CmaCh14G005570 vs. TrEMBL
Match: A0A067DGT9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.8e-39
Identity = 105/222 (47.30%), Postives = 130/222 (58.56%), Query Frame = 1

Query: 9   KSFSPGRLF--PSKKHRTSLSKSEPPSFGSASFS----------------SPTSVLPPHP 68
           +  SP RLF   SKK RTS+S+S+P SF S+S S                +PTSVL    
Sbjct: 5   EKLSPKRLFRSKSKKDRTSVSRSDPSSFSSSSTSDSSTHNKLGSAPDRVGTPTSVLH--- 64

Query: 69  HPHLSGEWSDFS-DTHIDY--------------------RKELEALLGLIGAEPPSEEEL 128
              +SG+WSD S D  +D                     R ELEALL  +GA+PP++EE+
Sbjct: 65  --EISGDWSDMSADISLDMNYELVQACKLLDRDNDGVVLRSELEALLIRLGADPPTQEEV 124

Query: 129 KMMMSQVDKVG-------------------PACHSELRDTFEIFDADHDGRITAEELFSV 173
           K M+S+VD+ G                   PAC  EL++TF+ FDADHDG+ITAEELF V
Sbjct: 125 KSMLSEVDREGDGYIPLEALISRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGV 184

BLAST of CmaCh14G005570 vs. TrEMBL
Match: V4SC13_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005842mg PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.8e-39
Identity = 105/222 (47.30%), Postives = 130/222 (58.56%), Query Frame = 1

Query: 9   KSFSPGRLF--PSKKHRTSLSKSEPPSFGSASFS----------------SPTSVLPPHP 68
           +  SP RLF   SKK RTS+S+S+P SF S+S S                +PTSVL    
Sbjct: 5   EKLSPKRLFRSKSKKDRTSVSRSDPSSFSSSSTSDSSTHNKLGSAPDRVGTPTSVLH--- 64

Query: 69  HPHLSGEWSDFS-DTHIDY--------------------RKELEALLGLIGAEPPSEEEL 128
              +SG+WSD S D  +D                     R ELEALL  +GA+PP++EE+
Sbjct: 65  --EISGDWSDMSADISLDMNYELVQACKLLDRDNDGVVLRSELEALLIRLGADPPTQEEV 124

Query: 129 KMMMSQVDKVG-------------------PACHSELRDTFEIFDADHDGRITAEELFSV 173
           K M+S+VD+ G                   PAC  EL++TF+ FDADHDG+ITAEELF V
Sbjct: 125 KSMLSEVDREGDGYIPLEALISRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGV 184

BLAST of CmaCh14G005570 vs. TAIR10
Match: AT3G10190.1 (AT3G10190.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 130.2 bits (326), Expect = 1.2e-30
Identity = 90/214 (42.06%), Postives = 119/214 (55.61%), Query Frame = 1

Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPP-SFGSASFSS-----------------P 60
           MKL K+      P R F   K R+++SKS    SFGSAS SS                 P
Sbjct: 1   MKLAKLI-----PKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTP 60

Query: 61  TSVLPPHPHPHLSGE------WSDFSDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQV 120
           TS+LP  P P+   E        D  +     R +LE+LL  +G +P +EEE+ +M+ +V
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEV 120

Query: 121 DKVG-----------------PACHS-ELRDTFEIFDADHDGRITAEELFSVFSAMGDEQ 173
           D  G                 PA  S EL++TFE FDAD DG I+A+EL  VFS +GDE+
Sbjct: 121 DCDGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

BLAST of CmaCh14G005570 vs. TAIR10
Match: AT2G41410.1 (AT2G41410.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 122.5 bits (306), Expect = 2.6e-28
Identity = 81/198 (40.91%), Postives = 106/198 (53.54%), Query Frame = 1

Query: 12  SPGRLFPSKKHRTSLSKSEPPSFGSASFSS----------------PTSVLPPHPHPHLS 71
           SP RLF +K  + S+S+SEP SF S + SS                P SVLP +     +
Sbjct: 11  SPKRLFRTKS-KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISVLPQNSGDFYT 70

Query: 72  G-----EWSDFSDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHS----- 131
                 +  D  D  +  R +L AL+  +  EPPS+EE+ +M+ +VD     C S     
Sbjct: 71  ELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCISLEDLA 130

Query: 132 ---------------ELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAG 169
                          ELR+ FEIFD D +G+I+AEEL  VF  +GDE+CTLE+C RMIA 
Sbjct: 131 SRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEECMRMIAT 190

BLAST of CmaCh14G005570 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 82.0 bits (201), Expect = 3.9e-16
Identity = 45/118 (38.14%), Postives = 70/118 (59.32%), Query Frame = 1

Query: 70  KELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHS-------------------ELRDT 129
           KEL A++  +G E P EEEL+  ++++D+ G    +                    L+D 
Sbjct: 56  KELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 115

Query: 130 FEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMM 169
           F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + FE+F +MM
Sbjct: 116 FSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEFKIMM 171

BLAST of CmaCh14G005570 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 74.3 bits (181), Expect = 8.1e-14
Identity = 46/129 (35.66%), Postives = 70/129 (54.26%), Query Frame = 1

Query: 63  DTHIDYRKELEALLGLIGAEPP--SEEELKMMMSQVDKVGP------------------- 122
           D + D +  ++ L  +IGA  P  S+EE K MM + D  G                    
Sbjct: 24  DKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSS 83

Query: 123 --ACHSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFV 169
             +   +L++ F+++D D +GRI+A EL SV   +G E+C+++DC+RMI  VD +GDG V
Sbjct: 84  NNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCV 143

BLAST of CmaCh14G005570 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 68.6 bits (166), Expect = 4.4e-12
Identity = 31/64 (48.44%), Postives = 50/64 (78.12%), Query Frame = 1

Query: 105 SELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDF 164
           S+L++ FE++D D +GRI+A+EL SV   +G E+C+++DC++MI+ VD +GDG V F++F
Sbjct: 93  SDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGCVNFDEF 152

Query: 165 AVMM 169
             MM
Sbjct: 153 KKMM 155

BLAST of CmaCh14G005570 vs. NCBI nr
Match: gi|659085286|ref|XP_008443339.1| (PREDICTED: probable calcium-binding protein CML35 [Cucumis melo])

HSP 1 Score: 249.2 bits (635), Expect = 5.2e-63
Identity = 128/172 (74.42%), Postives = 144/172 (83.72%), Query Frame = 1

Query: 9   KSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSS--------PTSVLPPHPHPHLSGEWSD 68
           KSF+P RLF SK HR+ LS+S+PPSFGSAS SS        PTSVLP       S +W+D
Sbjct: 70  KSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLP-------SLDWAD 129

Query: 69  FSDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDG 128
           FSD  +  RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGPACHSELRDTFEIFDADHDG
Sbjct: 130 FSDPTLS-RKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDG 189

Query: 129 RITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 173
           RITAEELFSVF+AMGDE CTLEDCRRMIAGVDKNGDGFVCF+DFA MM+CQR
Sbjct: 190 RITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 233

BLAST of CmaCh14G005570 vs. NCBI nr
Match: gi|778685391|ref|XP_004136671.2| (PREDICTED: probable calcium-binding protein CML35 [Cucumis sativus])

HSP 1 Score: 242.7 bits (618), Expect = 4.9e-61
Identity = 124/171 (72.51%), Postives = 141/171 (82.46%), Query Frame = 1

Query: 9   KSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSS-------PTSVLPPHPHPHLSGEWSDF 68
           KS +P RLF SK HR+ LS+S+PPSFGSAS SS       PTSVLP       S +WSDF
Sbjct: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSSAAGTPTSVLP-------SLDWSDF 64

Query: 69  SDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDGR 128
           SD  +  RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGP CHSELRDTFEIFDADHDGR
Sbjct: 65  SDPTLS-RKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGR 124

Query: 129 ITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 173
           ITAEELFSVF+AMGD+ CTLEDC+RMIAGVDKNGDGFVCF+DF  MM+CQR
Sbjct: 125 ITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

BLAST of CmaCh14G005570 vs. NCBI nr
Match: gi|645271060|ref|XP_008240735.1| (PREDICTED: probable calcium-binding protein CML36 [Prunus mume])

HSP 1 Score: 194.9 bits (494), Expect = 1.2e-46
Identity = 117/234 (50.00%), Postives = 145/234 (61.97%), Query Frame = 1

Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSK--SEPPSFGSASFSS----------------- 60
           MKL KI++KS SP  LF SKK R+++S+  S+PPSFGS + SS                 
Sbjct: 1   MKLIKISSKSLSPKHLFRSKKDRSAVSRRSSDPPSFGSGTASSSSSSSSSSDSLHKPGTG 60

Query: 61  --------PTSVLPPHPHPHLSGEWSDFS-DTHIDY----------------RKELEALL 120
                   PTSVLP       SG+WSDFS D  +D                 RKELEALL
Sbjct: 61  AEAGGIRTPTSVLPER-----SGDWSDFSTDLQLDMAQAFKLIDRDNDGVVSRKELEALL 120

Query: 121 GLIGAEPPSEEELKMMMSQVDK------------------VGPACHSELRDTFEIFDADH 173
             +GA+PPS+EE+ +M+S+VD+                   GPA  SELRD F++FD+DH
Sbjct: 121 SRLGADPPSQEEVMLMLSEVDREGNGSITLDALLNRVGPVCGPAADSELRDAFKVFDSDH 180

BLAST of CmaCh14G005570 vs. NCBI nr
Match: gi|595806938|ref|XP_007202521.1| (hypothetical protein PRUPE_ppa010940mg [Prunus persica])

HSP 1 Score: 184.9 bits (468), Expect = 1.2e-43
Identity = 114/229 (49.78%), Postives = 142/229 (62.01%), Query Frame = 1

Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSK--SEPPSF--GSASFSSPTSVLPPHPHP---- 60
           MKL KI++KS SP RLF SKK R+++S+  S+P SF  G+AS SS +S      H     
Sbjct: 1   MKLIKISSKSLSPKRLFRSKKDRSAVSRRSSDPLSFESGTASSSSSSSSSSDSLHKPGTG 60

Query: 61  --------------HLSGEWSDFS-DTHIDY----------------RKELEALLGLIGA 120
                           SG+WSDFS D  +D                 RKELEALL  +GA
Sbjct: 61  AEAGGIGTPTSVLLERSGDWSDFSTDLQLDMAQAFKLIDRDNDGVVSRKELEALLSRLGA 120

Query: 121 EPPSEEELKMMMSQVDK------------------VGPACHSELRDTFEIFDADHDGRIT 173
           +PPS+EE+ +M+S+VD+                   GPA  SELRD FE+FD+DHDG+I+
Sbjct: 121 DPPSQEEVMLMLSEVDREGNGSISLEALLSRVGPVCGPAADSELRDAFEVFDSDHDGKIS 180

BLAST of CmaCh14G005570 vs. NCBI nr
Match: gi|694428823|ref|XP_009341958.1| (PREDICTED: LOW QUALITY PROTEIN: probable calcium-binding protein CML36, partial [Pyrus x bretschneideri])

HSP 1 Score: 184.1 bits (466), Expect = 2.1e-43
Identity = 108/220 (49.09%), Postives = 135/220 (61.36%), Query Frame = 1

Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSSPTS---------------V 60
           MKL KI+ K+ SP RLF SKK R+S+S+SEPPSFGS + SS +S                
Sbjct: 72  MKLIKISPKNLSPKRLFGSKKDRSSVSRSEPPSFGSGTDSSSSSSSDSIHKPGAGAGGIT 131

Query: 61  LPPHPHPHLSGEWSDFS---------------DTHIDYRKELEALLGLIGAEPPSEEELK 120
            P    P  SG+WSDFS               +  +  RKELEALL  +G  PPS+EE+ 
Sbjct: 132 TPTSVLPEKSGDWSDFSADLDMAQAFKLIDRDNDGVVSRKELEALLCRLGNAPPSQEEVT 191

Query: 121 MMMSQVDK------------------VGPACHSELRDTFEIFDADHDGRITAEELFSVFS 173
           +M+S+VD+                   GPA  SELRD FE+FD D+DG+I+AEEL  VF+
Sbjct: 192 LMLSEVDRDGNGSITLEALLNQVGPVSGPAADSELRDAFEVFDTDNDGKISAEELLRVFT 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CML36_ARATH2.2e-2942.06Probable calcium-binding protein CML36 OS=Arabidopsis thaliana GN=CML36 PE=2 SV=... [more]
CML35_ARATH4.6e-2740.91Probable calcium-binding protein CML35 OS=Arabidopsis thaliana GN=CML35 PE=2 SV=... [more]
CML25_ARATH6.9e-1538.14Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
CML10_ORYSJ2.2e-1334.88Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ... [more]
CML23_ARATH1.4e-1235.66Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LFY7_CUCSA3.4e-6172.51Uncharacterized protein OS=Cucumis sativus GN=Csa_3G823060 PE=4 SV=1[more]
M5WA96_PRUPE8.4e-4449.78Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010940mg PE=4 SV=1[more]
A0A067D4X5_CITSI4.3e-4049.30Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1[more]
A0A067DGT9_CITSI4.8e-3947.30Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1[more]
V4SC13_9ROSI4.8e-3947.30Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005842mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10190.11.2e-3042.06 Calcium-binding EF-hand family protein[more]
AT2G41410.12.6e-2840.91 Calcium-binding EF-hand family protein[more]
AT1G24620.13.9e-1638.14 EF hand calcium-binding protein family[more]
AT1G66400.18.1e-1435.66 calmodulin like 23[more]
AT5G37770.14.4e-1248.44 EF hand calcium-binding protein family[more]
Match NameE-valueIdentityDescription
gi|659085286|ref|XP_008443339.1|5.2e-6374.42PREDICTED: probable calcium-binding protein CML35 [Cucumis melo][more]
gi|778685391|ref|XP_004136671.2|4.9e-6172.51PREDICTED: probable calcium-binding protein CML35 [Cucumis sativus][more]
gi|645271060|ref|XP_008240735.1|1.2e-4650.00PREDICTED: probable calcium-binding protein CML36 [Prunus mume][more]
gi|595806938|ref|XP_007202521.1|1.2e-4349.78hypothetical protein PRUPE_ppa010940mg [Prunus persica][more]
gi|694428823|ref|XP_009341958.1|2.1e-4349.09PREDICTED: LOW QUALITY PROTEIN: probable calcium-binding protein CML36, partial ... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G005570.1CmaCh14G005570.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 106..169
score: 3.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 106..134
score: 3.1E-5coord: 143..171
score: 0.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 102..137
score: 12.979coord: 139..173
score: 9
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 91..168
score: 1.8
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 70..169
score: 5.87
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 152..164
score: -coord: 115..127
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 69..169
score: 3.9
NoneNo IPR availablePANTHERPTHR10891:SF590SUBFAMILY NOT NAMEDcoord: 69..169
score: 3.9