CsGy3G035950 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy3G035950
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	probable calcium-binding protein CML35
Location	Chr3 : 34296020 .. 34296523 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTTCTCAAATCCTTAAACCCCAACCGTCTCTTCTCTTCCAAATCCCACCGCTCCAAACTCTCCCGATCTCAACCTCCTTCTTTCGGCTCTGCCTCCTCCTCCTCCTCCTCCTCCGCGGCAGGAACCCCCACCAGCGTTCTACCCTCCCTCGACTGGTCCGATTTCTCCGACCCCACTCTCTCTCGTAAAGAACTAAAGGCTCTACTCGGCCTAATCGGAGCCGAACCGCCGAGTGAGGAAGAGATTAAGATCATGATGGGCGAAATGGACCGGGTTGGACCGACTTGCCACTCCGAGTTACGTGATACGTTTGAGATATTCGACGCGGACCACGACGGGAGAATTACGGCGGAGGAGCTTTTTAGTGTGTTCGCAGCTATGGGAGACGATGGGTGCACGTTAGAGGATTGTCAGCGTATGATAGCAGGTGTAGACAAAAATGGGGATGGGTTCGTCTGCTTCGACGACTTTGTGCGTATGATGGACTGCCAGAGATAA

mRNA sequence

ATGAAGCTTCTCAAATCCTTAAACCCCAACCGTCTCTTCTCTTCCAAATCCCACCGCTCCAAACTCTCCCGATCTCAACCTCCTTCTTTCGGCTCTGCCTCCTCCTCCTCCTCCTCCTCCGCGGCAGGAACCCCCACCAGCGTTCTACCCTCCCTCGACTGGTCCGATTTCTCCGACCCCACTCTCTCTCGTAAAGAACTAAAGGCTCTACTCGGCCTAATCGGAGCCGAACCGCCGAGTGAGGAAGAGATTAAGATCATGATGGGCGAAATGGACCGGGTTGGACCGACTTGCCACTCCGAGTTACGTGATACGTTTGAGATATTCGACGCGGACCACGACGGGAGAATTACGGCGGAGGAGCTTTTTAGTGTGTTCGCAGCTATGGGAGACGATGGGTGCACGTTAGAGGATTGTCAGCGTATGATAGCAGGTGTAGACAAAAATGGGGATGGGTTCGTCTGCTTCGACGACTTTGTGCGTATGATGGACTGCCAGAGATAA

Coding sequence (CDS)

ATGAAGCTTCTCAAATCCTTAAACCCCAACCGTCTCTTCTCTTCCAAATCCCACCGCTCCAAACTCTCCCGATCTCAACCTCCTTCTTTCGGCTCTGCCTCCTCCTCCTCCTCCTCCTCCGCGGCAGGAACCCCCACCAGCGTTCTACCCTCCCTCGACTGGTCCGATTTCTCCGACCCCACTCTCTCTCGTAAAGAACTAAAGGCTCTACTCGGCCTAATCGGAGCCGAACCGCCGAGTGAGGAAGAGATTAAGATCATGATGGGCGAAATGGACCGGGTTGGACCGACTTGCCACTCCGAGTTACGTGATACGTTTGAGATATTCGACGCGGACCACGACGGGAGAATTACGGCGGAGGAGCTTTTTAGTGTGTTCGCAGCTATGGGAGACGATGGGTGCACGTTAGAGGATTGTCAGCGTATGATAGCAGGTGTAGACAAAAATGGGGATGGGTTCGTCTGCTTCGACGACTTTGTGCGTATGATGGACTGCCAGAGATAA

Protein sequence

MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSSAAGTPTSVLPSLDWSDFSDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR

Homology

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BLAST of CsGy3G035950 vs. NCBI nr
Match: XP_004136671.2 (PREDICTED: probable calcium-binding protein CML35 [Cucumis sativus] >KGN59507.1 hypothetical protein Csa_3G823060 [Cucumis sativus])

HSP 1 Score: 310.1 bits (793), Expect = 4.7e-81
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLPSLDWSDFSDP 60
           MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLPSLDWSDFSDP
Sbjct: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLPSLDWSDFSDP 60

Query: 61  TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE 120
           TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE
Sbjct: 61  TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE 120

Query: 121 ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 168
           ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR
Sbjct: 121 ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

[Close]

BLAST of CsGy3G035950 vs. NCBI nr
Match: XP_008443339.2 (PREDICTED: probable calcium-binding protein CML35 [Cucumis melo])

HSP 1 Score: 284.6 bits (727), Expect = 2.1e-73
Identity = 156/168 (92.86%), Postives = 159/168 (94.64%), Query Frame = 0


Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSF-GXXXXXXXXXXXXXPTSVLPSLDWSDFSD 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSF  XXXXXXXXXXXXX   VLPSLDW+DFSD
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFXXXXXXXXXXXXXXXXXXVLPSLDWADFSD 60

Query: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITA 120
           PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGP CHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 168
           EELFSVF AMGD+GCTLEDC+RMIAGVDKNGDGFVCFDDF RMMDCQR
Sbjct: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

[Close]

BLAST of CsGy3G035950 vs. NCBI nr
Match: XP_022983878.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 4.2e-58
Identity = 119/171 (69.59%), Postives = 136/171 (79.53%), Query Frame = 0


Query: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLP-------SLDWSDF 64
           KS +P RLF SK HR+ LS+S+PPSFG             PTSVLP       S +WSDF
Sbjct: 21  KSFSPGRLFPSKKHRTSLSKSEPPSFG-------SASFSSPTSVLPPHPHPHLSGEWSDF 80

Query: 65  SDPTLS-RKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGR 124
           SD  +  RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGP CHSELRDTFEIFDADHDGR
Sbjct: 81  SDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDGR 140

Query: 125 ITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 168
           ITAEELFSVF+AMGD+ CTLEDC+RMIAGVDKNGDGFVCF+DF  MM+CQR
Sbjct: 141 ITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 184

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BLAST of CsGy3G035950 vs. NCBI nr
Match: XP_022935658.1 (probable calcium-binding protein CML36 [Cucurbita moschata])

HSP 1 Score: 233.0 bits (593), Expect = 7.2e-58
Identity = 120/171 (70.18%), Postives = 136/171 (79.53%), Query Frame = 0


Query: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLP-------SLDWSDF 64
           KS +P RLF SK HR+ LS+S+PPSFG             PTSVLP       S +WSDF
Sbjct: 21  KSFSPGRLFPSKKHRTSLSKSEPPSFG-------SASSSSPTSVLPPHXXXXLSGEWSDF 80

Query: 65  SDP-TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGR 124
           SD  T  RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGP CHSELRDTFEIFDADHDGR
Sbjct: 81  SDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGR 140

Query: 125 ITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 168
           ITAEELFSVF+AMGD+ CTLEDC+RMIAGVDKNGDGFVCF+DF  MM+CQR
Sbjct: 141 ITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 184

[Close]

BLAST of CsGy3G035950 vs. NCBI nr
Match: XP_023527899.1 (probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 224.9 bits (572), Expect = 2.0e-55
Identity = 120/175 (68.57%), Postives = 135/175 (77.14%), Query Frame = 0


Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLP-------SLD 60
           MKL K L     F SK HRS LS+S+PPSFG             PTSVLP       S +
Sbjct: 13  MKLPK-LTAKSFFPSKKHRSSLSKSEPPSFG-------SASSSSPTSVLPPHXXXXLSGE 72

Query: 61  WSDFSDP-TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDAD 120
           WSDFSD  T  RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGP CHSELRDTFEIFDAD
Sbjct: 73  WSDFSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDAD 132

Query: 121 HDGRITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 168
           HDG+ITAEELFSVF+AMGD+ CTLEDC+RMIAGVDKNGDGFVCF+DF  MM+CQR
Sbjct: 133 HDGKITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 179

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BLAST of CsGy3G035950 vs. TAIR10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 107.8 bits (268), Expect = 6.4e-24
Identity = 79/209 (37.80%), Postives = 99/209 (47.37%), Query Frame = 0


Query: 1   MKLLKSLN---PNRLFSSKSHRSKLSRSQPPSF---------GXXXXXXXXXXXXXPTSV 60
           MKL  SLN   P RLF +KS ++ +SRS+P SF                       P SV
Sbjct: 1   MKLAASLNRLSPKRLFRTKS-KASVSRSEPSSFXXXXXXXXXXGSYGNLKQGPTATPISV 60

Query: 61  LPSLDWSDF--------------SDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRV 120
           LP  +  DF               D  +SR +L AL+  +  EPPS+EE+ +M+ E+D  
Sbjct: 61  LPQ-NSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGG 120

Query: 121 GPTCHS--------------------ELRDTFEIFDADHDGRITAEELFSVFAAMGDDGC 164
              C S                    ELR+ FEIFD D +G             +GD+ C
Sbjct: 121 DGGCISLEDXXXRVAGTXXEGSVETEELREVFEIFDVDRNGXXXXXXXXXXXXXIGDERC 180

[Close]

BLAST of CsGy3G035950 vs. TAIR10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 100.1 bits (248), Expect = 1.3e-21
Identity = 44/68 (64.71%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 100 SELRDTFEIFDADHDGRITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDF 159
           +EL++TFE FDAD DG I+A+EL  VF+ +GD+ CTL+DC+RMIA VD++GDGFVCF +F
Sbjct: 142 TELKETFEFFDADRDGLISADELLRVFSTIGDERCTLDDCKRMIADVDEDGDGFVCFTEF 201

Query: 160 VRMMDCQR 168
            RMMD QR
Sbjct: 202 SRMMDLQR 209

[Close]

BLAST of CsGy3G035950 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 77.0 bits (188), Expect = 1.2e-14
Identity = 45/124 (36.29%), Postives = 70/124 (56.45%), Query Frame = 0


Query: 59  DPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGP-------------------TCH 118
           D  +S KEL A++  +G E P EEE++  + E+DR G                       
Sbjct: 50  DGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 119 SELRDTFEIFDADHDGRITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDF 164
             L+D F ++D D +G I+AEEL  V  ++GD+ C++ +C++MI GVDK+GDG + F++F
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169

[Close]

BLAST of CsGy3G035950 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 70.9 bits (172), Expect = 8.6e-13
Identity = 47/125 (37.60%), Postives = 65/125 (52.00%), Query Frame = 0


Query: 59  DPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTC-------------------H 118
           D  +++ ELK     +G   P E EI  M+ +MD  G                       
Sbjct: 18  DGKIAKNELKDFFKSVGIMVP-ENEINEMIAKMDVNGDGAMDIDEFGSLYQEMVEEKEEE 77

Query: 119 SELRDTFEIFDADHDGRITAEELFSVFAAMG-DDGCTLEDCQRMIAGVDKNGDGFVCFDD 164
            ++R+ F +FD + DG IT EEL SV A+MG   G TLEDC++MI+ VD +GDG V F +
Sbjct: 78  EDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDGDGMVNFKE 137

[Close]

BLAST of CsGy3G035950 vs. TAIR10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 68.6 bits (166), Expect = 4.3e-12
Identity = 51/171 (29.82%), Postives = 77/171 (45.03%), Query Frame = 0


Query: 9   PNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLPSLDWSDFSDPTLSRKELK 68
           P+R+FS     S LS   P  F                 V    D     D  +S+ E K
Sbjct: 22  PSRMFSRDRQSSGLSSPGPGGFSQPSVNEM-------RRVFSRFDLD--KDGKISQTEYK 81

Query: 69  ALLGLIGAEPPSEE----------------EIKIMMGEMDRVGPTCHSELRDTFEIFDAD 128
            +L  +G E   E+                  +  +    R G    S++R++F  FD +
Sbjct: 82  VVLRALGQERAIEDXXXXXXXXXXXXXXXXXFREFIDAYKRSGGIRSSDIRNSFWTFDLN 141

Query: 129 HDGRITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMM 164
            DG+I+AEE+ SV   +G + C+LEDC RM+  VD +GDG V  ++F++MM
Sbjct: 142 GDGKISAEEVMSVLWKLG-ERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

[Close]

BLAST of CsGy3G035950 vs. Swiss-Prot
Match: sp|P30188|CML35_ARATH (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 79/209 (37.80%), Postives = 99/209 (47.37%), Query Frame = 0


Query: 1   MKLLKSLN---PNRLFSSKSHRSKLSRSQPPSF---------GXXXXXXXXXXXXXPTSV 60
           MKL  SLN   P RLF +KS ++ +SRS+P SF                       P SV
Sbjct: 1   MKLAASLNRLSPKRLFRTKS-KASVSRSEPSSFXXXXXXXXXXGSYGNLKQGPTATPISV 60

Query: 61  LPSLDWSDF--------------SDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRV 120
           LP  +  DF               D  +SR +L AL+  +  EPPS+EE+ +M+ E+D  
Sbjct: 61  LPQ-NSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGG 120

Query: 121 GPTCHS--------------------ELRDTFEIFDADHDGRITAEELFSVFAAMGDDGC 164
              C S                    ELR+ FEIFD D +G             +GD+ C
Sbjct: 121 DGGCISLEDXXXRVAGTXXEGSVETEELREVFEIFDVDRNGXXXXXXXXXXXXXIGDERC 180

[Close]

BLAST of CsGy3G035950 vs. Swiss-Prot
Match: sp|Q9SS31|CML36_ARATH (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.4e-20
Identity = 44/68 (64.71%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 100 SELRDTFEIFDADHDGRITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDF 159
           +EL++TFE FDAD DG I+A+EL  VF+ +GD+ CTL+DC+RMIA VD++GDGFVCF +F
Sbjct: 142 TELKETFEFFDADRDGLISADELLRVFSTIGDERCTLDDCKRMIADVDEDGDGFVCFTEF 201

Query: 160 VRMMDCQR 168
            RMMD QR
Sbjct: 202 SRMMDLQR 209

[Close]

BLAST of CsGy3G035950 vs. Swiss-Prot
Match: sp|Q5ZCK5|CML16_ORYSJ (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 GN=CML16 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.7e-13
Identity = 51/128 (39.84%), Postives = 68/128 (53.12%), Query Frame = 0


Query: 59  DPTLSRKELKALLGLIGAEPPSE----EEIKIMMGEMD-------------------RVG 118
           D  +S  EL A+   I A PPSE     E+  MM E+D                   R  
Sbjct: 40  DGRISPSELAAVTRAI-APPPSESAGGREVAAMMNELDTDRDGFVDLGEFAAFHGRGRGD 99

Query: 119 PTCHSELRDTFEIFDADHDGRITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVC 164
               +ELR  F+++D D DGRITA EL  V   +G +GC+ E+C+RMIA VD +GDG V 
Sbjct: 100 AEHEAELRAAFDVYDVDGDGRITAAELGKVLGRIG-EGCSAEECERMIASVDVDGDGCVG 159

[Close]

BLAST of CsGy3G035950 vs. Swiss-Prot
Match: sp|Q9FYK2|CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 45/124 (36.29%), Postives = 70/124 (56.45%), Query Frame = 0


Query: 59  DPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGP-------------------TCH 118
           D  +S KEL A++  +G E P EEE++  + E+DR G                       
Sbjct: 50  DGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 119 SELRDTFEIFDADHDGRITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDF 164
             L+D F ++D D +G I+AEEL  V  ++GD+ C++ +C++MI GVDK+GDG + F++F
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169

[Close]

BLAST of CsGy3G035950 vs. Swiss-Prot
Match: sp|Q0DJV6|CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-12
Identity = 49/136 (36.03%), Postives = 69/136 (50.74%), Query Frame = 0


Query: 59  DPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMD---------------RVGPT------ 118
           D  +S +EL ++L  +GA P    E++ MM EMD                 GPT      
Sbjct: 18  DGKISAEELASVLRALGA-PLGPGEVRRMMDEMDSDRDGFVDLSEFAAFHCGPTPAHGGK 77

Query: 119 ----------CHSELRDTFEIFDADHDGRITAEELFSVFAAMGDDGCTLEDCQRMIAGVD 164
                       +ELR+ F ++DAD +G+I+A EL  V   +GD  C++ DC RMI  VD
Sbjct: 78  GGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDK-CSVADCSRMIRSVD 137

[Close]

BLAST of CsGy3G035950 vs. TrEMBL
Match: tr|A0A0A0LFY7|A0A0A0LFY7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823060 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.1e-81
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLPSLDWSDFSDP 60
           MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLPSLDWSDFSDP
Sbjct: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLPSLDWSDFSDP 60

Query: 61  TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE 120
           TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE
Sbjct: 61  TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE 120

Query: 121 ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 168
           ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR
Sbjct: 121 ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

[Close]

BLAST of CsGy3G035950 vs. TrEMBL
Match: tr|A0A1S3B7R8|A0A1S3B7R8_CUCME (probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.4e-73
Identity = 156/168 (92.86%), Postives = 159/168 (94.64%), Query Frame = 0


Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSF-GXXXXXXXXXXXXXPTSVLPSLDWSDFSD 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSF  XXXXXXXXXXXXX   VLPSLDW+DFSD
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFXXXXXXXXXXXXXXXXXXVLPSLDWADFSD 60

Query: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITA 120
           PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGP CHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 168
           EELFSVF AMGD+GCTLEDC+RMIAGVDKNGDGFVCFDDF RMMDCQR
Sbjct: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

[Close]

BLAST of CsGy3G035950 vs. TrEMBL
Match: tr|A0A2P6RTD5|A0A2P6RTD5_ROSCH (Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0124821 PE=4 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 9.7e-43
Identity = 110/219 (50.23%), Postives = 129/219 (58.90%), Query Frame = 0


Query: 1   MKLLK--SLNPNRLFSSKSHRSKLSRSQPPSFG------------XXXXXXXXXXXXXPT 60
           MKL+K  SL+P RLF S   RS +SRS PPSFG                         PT
Sbjct: 1   MKLMKINSLSPKRLFRSMKDRSAVSRSDPPSFGSGTASSSSSDPLPSDHKPGPGGMVTPT 60

Query: 61  SVLPSL--DWSDFS------------------DPTLSRKELKALLGLIGAEPPSEEEIKI 120
           SVLP +  DWSDFS                  D  +SRKEL+ALL  +GAEPPS EE+ +
Sbjct: 61  SVLPEISGDWSDFSADTHLDMAQAFKLIDRDNDGVVSRKELEALLKRLGAEPPSHEEVAM 120

Query: 121 MMGEMD--------------RVGPTCH----SELRDTFEIFDADHDGRITAEELFSVFAA 168
           M+ E+D              R GP C     SELR+ FE+FDADHDG+I+AEELFSVF A
Sbjct: 121 MLSEVDRDGDGCIPLEALLHRFGPACEPAADSELREAFEVFDADHDGKISAEELFSVFTA 180

[Close]

BLAST of CsGy3G035950 vs. TrEMBL
Match: tr|A0A2I4ECU2|A0A2I4ECU2_9ROSI (probable calcium-binding protein CML36 OS=Juglans regia OX=51240 GN=LOC108988409 PE=4 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.0e-40
Identity = 104/215 (48.37%), Postives = 122/215 (56.74%), Query Frame = 0


Query: 7   LNPNRLFSSKSHRSKLSRSQPPSFG----------------XXXXXXXXXXXXXPTSVLP 66
           ++P RLF SK  RS +SRS P SFG                             P SVLP
Sbjct: 16  VSPTRLFRSKKDRSSVSRSDPSSFGSXXXXXXXXXXXXSHQKPGSGVGITNLGTPKSVLP 75

Query: 67  SL--DWSDFS------------------DPTLSRKELKALLGLIGAEPPSEEEIKIMMGE 126
            +  +WSD S                  D  +SRKEL+ALL  IGAEP SEEE+K+M+ E
Sbjct: 76  EILGNWSDSSADMNVELAQAFRLTDRDDDGLVSRKELQALLSRIGAEPLSEEEVKMMLNE 135

Query: 127 MDR------------------VGPTCHSELRDTFEIFDADHDGRITAEELFSVFAAMGDD 168
           +DR                   GP C SELR+TF+IFDADHDGRITAEEL+  F AMGD+
Sbjct: 136 VDRDGDGHISLETLLSRFGSACGPACDSELRETFDIFDADHDGRITAEELWGFFTAMGDE 195

[Close]

BLAST of CsGy3G035950 vs. TrEMBL
Match: tr|M5WA96|M5WA96_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G047300 PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.8e-39
Identity = 109/229 (47.60%), Postives = 130/229 (56.77%), Query Frame = 0


Query: 1   MKLL----KSLNPNRLFSSKSHRSKLSR--SQPPSF------------------GXXXXX 60
           MKL+    KSL+P RLF SK  RS +SR  S P SF                        
Sbjct: 1   MKLIKISSKSLSPKRLFRSKKDRSAVSRRSSDPLSFESGTXXXXXXXXXXXXXLHKPGTG 60

Query: 61  XXXXXXXXPTSVL--PSLDWSDFS------------------DPTLSRKELKALLGLIGA 120
                   PTSVL   S DWSDFS                  D  +SRKEL+ALL  +GA
Sbjct: 61  AEAGGIGTPTSVLLERSGDWSDFSTDLQLDMAQAFKLIDRDNDGVVSRKELEALLSRLGA 120

Query: 121 EPPSEEEIKIMMGEMD--------------RVGPTC----HSELRDTFEIFDADHDGRIT 168
           +PPS+EE+ +M+ E+D              RVGP C     SELRD FE+FD+DHDG+I+
Sbjct: 121 DPPSQEEVMLMLSEVDREGNGSISLEALLSRVGPVCGPAADSELRDAFEVFDSDHDGKIS 180

The following BLAST results are available for this feature:

BLAST of CsGy3G035950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136671.2	4.7e-81	100.00	PREDICTED: probable calcium-binding protein CML35 [Cucumis sativus] >KGN59507.1 ...	[more]
XP_008443339.2	2.1e-73	92.86	PREDICTED: probable calcium-binding protein CML35 [Cucumis melo]	[more]
XP_022983878.1	4.2e-58	69.59	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]
XP_022935658.1	7.2e-58	70.18	probable calcium-binding protein CML36 [Cucurbita moschata]	[more]
XP_023527899.1	2.0e-55	68.57	probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsGy3G035950 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G41410.1	6.4e-24	37.80	Calcium-binding EF-hand family protein	[more]
AT3G10190.1	1.3e-21	64.71	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	1.2e-14	36.29	EF hand calcium-binding protein family	[more]
AT4G12860.1	8.6e-13	37.60	EF hand calcium-binding protein family	[more]
AT2G15680.1	4.3e-12	29.82	Calcium-binding EF-hand family protein	[more]

BLAST of CsGy3G035950 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P30188\|CML35_ARATH	1.2e-22	37.80	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
sp\|Q9SS31\|CML36_ARATH	2.4e-20	64.71	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
sp\|Q5ZCK5\|CML16_ORYSJ	1.7e-13	39.84	Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q9FYK2\|CML25_ARATH	2.2e-13	36.29	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
sp\|Q0DJV6\|CML18_ORYSJ	1.4e-12	36.03	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CsGy3G035950 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LFY7\|A0A0A0LFY7_CUCSA	3.1e-81	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823060 PE=4 SV=1	[more]
tr\|A0A1S3B7R8\|A0A1S3B7R8_CUCME	1.4e-73	92.86	probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 P...	[more]
tr\|A0A2P6RTD5\|A0A2P6RTD5_ROSCH	9.7e-43	50.23	Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2...	[more]
tr\|A0A2I4ECU2\|A0A2I4ECU2_9ROSI	2.0e-40	48.37	probable calcium-binding protein CML36 OS=Juglans regia OX=51240 GN=LOC108988409...	[more]
tr\|M5WA96\|M5WA96_PRUPE	3.8e-39	47.60	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G047300 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G035950.1	CsGy3G035950.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 101..129 e-value: 3.4E-5 score: 33.3 coord: 138..166 e-value: 0.0017 score: 27.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 101..163 e-value: 2.5E-9 score: 37.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 61..77 score: 5.392
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 97..132 score: 12.923
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 134..167 score: 11.668
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 101..163 e-value: 3.04326E-18 score: 74.1213
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 56..167 e-value: 4.5E-21 score: 77.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..48
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..48
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..51 coord: 95..167 coord: 59..98
None	No IPR available	PANTHER	PTHR10891:SF739	CALCIUM-BINDING PROTEIN CML36-RELATED	coord: 95..167 coord: 59..98
None	No IPR available	PANTHER	PTHR10891:SF739	CALCIUM-BINDING PROTEIN CML36-RELATED	coord: 1..51
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 110..122
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 147..159
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 56..163

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy3G035950	Cla016954	Watermelon (97103) v1	cgybwmB187
CsGy3G035950	Csa3G823060	Cucumber (Chinese Long) v2	cgybcuB110
CsGy3G035950	MELO3C009818	Melon (DHL92) v3.5.1	cgybmeB184
CsGy3G035950	ClCG10G015590	Watermelon (Charleston Gray)	cgybwcgB179
CsGy3G035950	CSPI03G38420	Wild cucumber (PI 183967)	cgybcpiB111
CsGy3G035950	CmaCh14G005570	Cucurbita maxima (Rimu)	cgybcmaB306
CsGy3G035950	CmoCh14G005610	Cucurbita moschata (Rifu)	cgybcmoB279
CsGy3G035950	Lsi03G015390	Bottle gourd (USVL1VR-Ls)	cgyblsiB171
CsGy3G035950	Cp4.1LG03g08270	Cucurbita pepo (Zucchini)	cgybcpeB395
CsGy3G035950	MELO3C009818.2	Melon (DHL92) v3.6.1	cgybmedB185
CsGy3G035950	CsaV3_3G040820	Cucumber (Chinese Long) v3	cgybcucB117
CsGy3G035950	Cla97C10G198500	Watermelon (97103) v2	cgybwmbB178
CsGy3G035950	Bhi11G002433	Wax gourd	cgybwgoB231
CsGy3G035950	Carg09817	Silver-seed gourd	carcgybB0444

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy3G035950	Wax gourd	cgybwgoB211
CsGy3G035950	Cucurbita maxima (Rimu)	cgybcmaB444
CsGy3G035950	Cucurbita moschata (Rifu)	cgybcmoB413
CsGy3G035950	Silver-seed gourd	carcgybB0329