Carg09817 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg09817
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	EF hand calcium-binding family protein
Location	Cucurbita_argyrosperma_scaffold_023 : 1111674 .. 1112195 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTCCCCAAAATCACCGCCAAATCCTTCTCCCCCGGCCGTCTTTTCCCATCCAAAAAGCACCGCACCAGCCTCTCCAAATCCGAGCCCCCTTCCTTCGGCTCCGCCTCATCCTCCTCCCCAACCAGCGTCTTACCGCCTCACCCGCACCCCCACCTCTCGGGAGAATGGTCCGACTTCTCCGACACCCACACCGACTACCGCAAGGAACTGGAAGCTCTACTAGGCCTCATCGGCGCCGAACCGCCGAGCGAAGAGGAGCTCAAGATGATGATGAGCCAAGTAGACAAAGTCGGCCCGCCTTGCCACTCCGAACTGCGAGATACGTTTGAAATCTTCGACGCCGACCACGACGGAAGAATCACGGCGGAGGAGCTTTTTAGTGTGTTCTCCGCCATGGGAGATGAGCAGTGCACGTTGGAGGATTGCCGGCGTATGATTGCCGGAGTGGACAAAAACGGGGATGGGTTCGTGTGCTTCGAGGACTTCGCGGTTATGATGGAGTGCCAGAGATGTTGA

mRNA sequence

ATGAAGCTCCCCAAAATCACCGCCAAATCCTTCTCCCCCGGCCGTCTTTTCCCATCCAAAAAGCACCGCACCAGCCTCTCCAAATCCGAGCCCCCTTCCTTCGGCTCCGCCTCATCCTCCTCCCCAACCAGCGTCTTACCGCCTCACCCGCACCCCCACCTCTCGGGAGAATGGTCCGACTTCTCCGACACCCACACCGACTACCGCAAGGAACTGGAAGCTCTACTAGGCCTCATCGGCGCCGAACCGCCGAGCGAAGAGGAGCTCAAGATGATGATGAGCCAAGTAGACAAAGTCGGCCCGCCTTGCCACTCCGAACTGCGAGATACGTTTGAAATCTTCGACGCCGACCACGACGGAAGAATCACGGCGGAGGAGCTTTTTAGTGTGTTCTCCGCCATGGGAGATGAGCAGTGCACGTTGGAGGATTGCCGGCGTATGATTGCCGGAGTGGACAAAAACGGGGATGGGTTCGTGTGCTTCGAGGACTTCGCGGTTATGATGGAGTGCCAGAGATGTTGA

Coding sequence (CDS)

ATGAAGCTCCCCAAAATCACCGCCAAATCCTTCTCCCCCGGCCGTCTTTTCCCATCCAAAAAGCACCGCACCAGCCTCTCCAAATCCGAGCCCCCTTCCTTCGGCTCCGCCTCATCCTCCTCCCCAACCAGCGTCTTACCGCCTCACCCGCACCCCCACCTCTCGGGAGAATGGTCCGACTTCTCCGACACCCACACCGACTACCGCAAGGAACTGGAAGCTCTACTAGGCCTCATCGGCGCCGAACCGCCGAGCGAAGAGGAGCTCAAGATGATGATGAGCCAAGTAGACAAAGTCGGCCCGCCTTGCCACTCCGAACTGCGAGATACGTTTGAAATCTTCGACGCCGACCACGACGGAAGAATCACGGCGGAGGAGCTTTTTAGTGTGTTCTCCGCCATGGGAGATGAGCAGTGCACGTTGGAGGATTGCCGGCGTATGATTGCCGGAGTGGACAAAAACGGGGATGGGTTCGTGTGCTTCGAGGACTTCGCGGTTATGATGGAGTGCCAGAGATGTTGA

Protein sequence

MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSSPTSVLPPHPHPHLSGEWSDFSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC

Homology

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BLAST of Carg09817 vs. NCBI nr
Match: XP_022935658.1 (probable calcium-binding protein CML36 [Cucurbita moschata])

HSP 1 Score: 342.4 bits (877), Expect = 8.8e-91
Identity = 172/173 (99.42%), Postives = 173/173 (100.00%), Query Frame = 0


Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSSPTSVLPPHXXXXLSGEWSD 60
           MKLPK+TAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSSPTSVLPPHXXXXLSGEWSD
Sbjct: 13  MKLPKLTAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSSPTSVLPPHXXXXLSGEWSD 72

Query: 61  FSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDG 120
           FSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDG
Sbjct: 73  FSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDG 132

Query: 121 RITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC 174
           RITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC
Sbjct: 133 RITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC 185

[Close]

BLAST of Carg09817 vs. NCBI nr
Match: XP_022983878.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 335.5 bits (859), Expect = 1.1e-88
Identity = 166/173 (95.95%), Postives = 166/173 (95.95%), Query Frame = 0


Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSSPTSVLPPHXXXXLSGEWSD 60
           MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSAS SSPTSVLPPH    LSGEWSD
Sbjct: 13  MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSSPTSVLPPHPHPHLSGEWSD 72

Query: 61  FSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDG 120
           FSDTH DYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGP CHSELRDTFEIFDADHDG
Sbjct: 73  FSDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDG 132

Query: 121 RITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC 174
           RITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC
Sbjct: 133 RITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC 185

[Close]

BLAST of Carg09817 vs. NCBI nr
Match: XP_023527899.1 (probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 323.9 bits (829), Expect = 3.2e-85
Identity = 165/173 (95.38%), Postives = 168/173 (97.11%), Query Frame = 0


Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSSPTSVLPPHXXXXLSGEWSD 60
           MKLPK+TAKSF     FPSKKHR+SLSKSEPPSFGSASSSSPTSVLPPHXXXXLSGEWSD
Sbjct: 13  MKLPKLTAKSF-----FPSKKHRSSLSKSEPPSFGSASSSSPTSVLPPHXXXXLSGEWSD 72

Query: 61  FSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDG 120
           FSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDG
Sbjct: 73  FSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDG 132

Query: 121 RITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC 174
           +ITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC
Sbjct: 133 KITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQRC 180

[Close]

BLAST of Carg09817 vs. NCBI nr
Match: XP_004136671.2 (PREDICTED: probable calcium-binding protein CML35 [Cucumis sativus] >KGN59507.1 hypothetical protein Csa_3G823060 [Cucumis sativus])

HSP 1 Score: 230.7 bits (587), Expect = 3.7e-57
Identity = 120/171 (70.18%), Postives = 136/171 (79.53%), Query Frame = 0


Query: 9   KSFSPGRLFPSKKHRTSLSKSEPPSFG-------SASSSSPTSVLPPHXXXXLSGEWSDF 68
           KS +P RLF SK HR+ LS+S+PPSFG             PTSVLP       S +WSDF
Sbjct: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLP-------SLDWSDF 64

Query: 69  SDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGR 128
           SD  T  RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGP CHSELRDTFEIFDADHDGR
Sbjct: 65  SDP-TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGR 124

Query: 129 ITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 173
           ITAEELFSVF+AMGD+ CTLEDC+RMIAGVDKNGDGFVCF+DF  MM+CQR
Sbjct: 125 ITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

[Close]

BLAST of Carg09817 vs. NCBI nr
Match: XP_008443339.2 (PREDICTED: probable calcium-binding protein CML35 [Cucumis melo])

HSP 1 Score: 227.6 bits (579), Expect = 3.2e-56
Identity = 120/165 (72.73%), Postives = 135/165 (81.82%), Query Frame = 0


Query: 9   KSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSSPTSVLPPHXXXXL-SGEWSDFSDTHTD 68
           KSF+P RLF SK HR+ LS+S+PPSF               XXX L S +W+DFSD  T 
Sbjct: 5   KSFNPNRLFSSKSHRSKLSRSQPPSFXXXXXXXXXXXXXXXXXXVLPSLDWADFSDP-TL 64

Query: 69  YRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGRITAEEL 128
            RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGP CHSELRDTFEIFDADHDGRITAEEL
Sbjct: 65  SRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITAEEL 124

Query: 129 FSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 173
           FSVF+AMGDE CTLEDCRRMIAGVDKNGDGFVCF+DFA MM+CQR
Sbjct: 125 FSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

[Close]

BLAST of Carg09817 vs. TAIR10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 99.4 bits (246), Expect = 2.4e-21
Identity = 71/198 (35.86%), Postives = 95/198 (47.98%), Query Frame = 0


Query: 12  SPGRLFPSKKHRTSLSKSEPPSFG----------------SASSSSPTSVLPPHXXXXLS 71
           SP RLF +K  + S+S+SEP SF                    +++P SVLP +     +
Sbjct: 11  SPKRLFRTKS-KASVSRSEPSSFXXXXXXXXXXGSYGNLKQGPTATPISVLPQNSGDFYT 70

Query: 72  G-----EWSDFSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHS----- 131
                 +  D  D     R +L AL+  +  EPPS+EE+ +M+ +VD     C S     
Sbjct: 71  ELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCISLEDXX 130

Query: 132 ---------------ELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAG 169
                          ELR+ FEIFD D +G             +GDE+CTLE+C RMIA 
Sbjct: 131 XRVAGTXXEGSVETEELREVFEIFDVDRNGXXXXXXXXXXXXXIGDERCTLEECMRMIAT 190

[Close]

BLAST of Carg09817 vs. TAIR10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 98.2 bits (243), Expect = 5.2e-21
Identity = 76/214 (35.51%), Postives = 103/214 (48.13%), Query Frame = 0


Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPP-SFGSAS-----------------SSSP 60
           MKL K+      P R F   K R+++SKS    SFGSAS                 S +P
Sbjct: 1   MKLAKLI-----PKRFFIRSKDRSTVSKSPTAFSFGSASXXXXXXXXXXXXXXXXXSVTP 60

Query: 61  TSVLP----PHXXXXLSGEWS--DFSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQV 120
           TS+LP    P+    +   +   D  +     R +LE+LL  +G                
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGXXXXXXXXXXXXXXXX 120

Query: 121 DKVGPPC------------------HSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQ 173
                                     +EL++TFE FDAD DG I+A+EL  VFS +GDE+
Sbjct: 121 XXXXXXXXXXXXXXXXXXXLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

[Close]

BLAST of Carg09817 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 81.6 bits (200), Expect = 5.1e-16
Identity = 45/118 (38.14%), Postives = 69/118 (58.47%), Query Frame = 0


Query: 70  KELEALLGLIGAEPPSEEELKMMMSQVDKVGP-------------------PCHSELRDT 129
           KEL A++  +G E P EEEL+  ++++D+ G                         L+D 
Sbjct: 56  KELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 115

Query: 130 FEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMM 169
           F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + FE+F +MM
Sbjct: 116 FSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of Carg09817 vs. TAIR10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 65.5 bits (158), Expect = 3.8e-11
Identity = 31/64 (48.44%), Postives = 44/64 (68.75%), Query Frame = 0


Query: 105 SELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDF 164
           +E+RD F+++D D +G I+A EL  V + +G   C++EDC RMI  VD +GDG V FE+F
Sbjct: 91  AEIRDAFDLYDQDKNGLISASELHQVLNRLG-MSCSVEDCTRMIGPVDADGDGNVNFEEF 150

Query: 165 AVMM 169
             MM
Sbjct: 151 QKMM 153

[Close]

BLAST of Carg09817 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 65.1 bits (157), Expect = 4.9e-11
Identity = 33/64 (51.56%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 106 ELRDTFEIFDADHDGRITAEELFSVFSAMGDEQ-CTLEDCRRMIAGVDKNGDGFVCFEDF 165
           ++R+ F +FD + DG IT EEL SV ++MG +Q  TLEDC++MI+ VD +GDG V F++F
Sbjct: 78  DMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDGDGMVNFKEF 137

Query: 166 AVMM 169
             MM
Sbjct: 138 KQMM 141

[Close]

BLAST of Carg09817 vs. Swiss-Prot
Match: sp|P30188|CML35_ARATH (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 4.2e-20
Identity = 71/198 (35.86%), Postives = 95/198 (47.98%), Query Frame = 0


Query: 12  SPGRLFPSKKHRTSLSKSEPPSFG----------------SASSSSPTSVLPPHXXXXLS 71
           SP RLF +K  + S+S+SEP SF                    +++P SVLP +     +
Sbjct: 11  SPKRLFRTKS-KASVSRSEPSSFXXXXXXXXXXGSYGNLKQGPTATPISVLPQNSGDFYT 70

Query: 72  G-----EWSDFSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHS----- 131
                 +  D  D     R +L AL+  +  EPPS+EE+ +M+ +VD     C S     
Sbjct: 71  ELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCISLEDXX 130

Query: 132 ---------------ELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAG 169
                          ELR+ FEIFD D +G             +GDE+CTLE+C RMIA 
Sbjct: 131 XRVAGTXXEGSVETEELREVFEIFDVDRNGXXXXXXXXXXXXXIGDERCTLEECMRMIAT 190

[Close]

BLAST of Carg09817 vs. Swiss-Prot
Match: sp|Q9SS31|CML36_ARATH (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 9.4e-20
Identity = 76/214 (35.51%), Postives = 103/214 (48.13%), Query Frame = 0


Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPP-SFGSAS-----------------SSSP 60
           MKL K+      P R F   K R+++SKS    SFGSAS                 S +P
Sbjct: 1   MKLAKLI-----PKRFFIRSKDRSTVSKSPTAFSFGSASXXXXXXXXXXXXXXXXXSVTP 60

Query: 61  TSVLP----PHXXXXLSGEWS--DFSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQV 120
           TS+LP    P+    +   +   D  +     R +LE+LL  +G                
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGXXXXXXXXXXXXXXXX 120

Query: 121 DKVGPPC------------------HSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQ 173
                                     +EL++TFE FDAD DG I+A+EL  VFS +GDE+
Sbjct: 121 XXXXXXXXXXXXXXXXXXXLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

[Close]

BLAST of Carg09817 vs. Swiss-Prot
Match: sp|Q9FYK2|CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 9.1e-15
Identity = 45/118 (38.14%), Postives = 69/118 (58.47%), Query Frame = 0


Query: 70  KELEALLGLIGAEPPSEEELKMMMSQVDKVGP-------------------PCHSELRDT 129
           KEL A++  +G E P EEEL+  ++++D+ G                         L+D 
Sbjct: 56  KELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 115

Query: 130 FEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMM 169
           F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + FE+F +MM
Sbjct: 116 FSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of Carg09817 vs. Swiss-Prot
Match: sp|Q5ZCK5|CML16_ORYSJ (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 GN=CML16 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-11
Identity = 35/64 (54.69%), Postives = 44/64 (68.75%), Query Frame = 0


Query: 105 SELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDF 164
           +ELR  F+++D D DGRITA EL  V   +G E C+ E+C RMIA VD +GDG V FE+F
Sbjct: 103 AELRAAFDVYDVDGDGRITAAELGKVLGRIG-EGCSAEECERMIASVDVDGDGCVGFEEF 162

Query: 165 AVMM 169
             MM
Sbjct: 163 KKMM 165

[Close]

BLAST of Carg09817 vs. Swiss-Prot
Match: sp|Q6L4D4|CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.0e-10
Identity = 33/63 (52.38%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 106 ELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFA 165
           +LR  F +FDAD +G IT  EL  V   +G E  T+  CRRMI GVD+NGDG + FE+F 
Sbjct: 129 DLRHAFGVFDADGNGVITPAELARVLRGIG-EAATVAQCRRMIDGVDRNGDGLINFEEFK 188

Query: 166 VMM 169
           +MM
Sbjct: 189 LMM 190

[Close]

BLAST of Carg09817 vs. TrEMBL
Match: tr|A0A0A0LFY7|A0A0A0LFY7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823060 PE=4 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.5e-57
Identity = 120/171 (70.18%), Postives = 136/171 (79.53%), Query Frame = 0


Query: 9   KSFSPGRLFPSKKHRTSLSKSEPPSFG-------SASSSSPTSVLPPHXXXXLSGEWSDF 68
           KS +P RLF SK HR+ LS+S+PPSFG             PTSVLP       S +WSDF
Sbjct: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGXXXXXXXXXXXXXPTSVLP-------SLDWSDF 64

Query: 69  SDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGR 128
           SD  T  RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGP CHSELRDTFEIFDADHDGR
Sbjct: 65  SDP-TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGR 124

Query: 129 ITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 173
           ITAEELFSVF+AMGD+ CTLEDC+RMIAGVDKNGDGFVCF+DF  MM+CQR
Sbjct: 125 ITAEELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

[Close]

BLAST of Carg09817 vs. TrEMBL
Match: tr|A0A1S3B7R8|A0A1S3B7R8_CUCME (probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.1e-56
Identity = 120/165 (72.73%), Postives = 135/165 (81.82%), Query Frame = 0


Query: 9   KSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSSPTSVLPPHXXXXL-SGEWSDFSDTHTD 68
           KSF+P RLF SK HR+ LS+S+PPSF               XXX L S +W+DFSD  T 
Sbjct: 5   KSFNPNRLFSSKSHRSKLSRSQPPSFXXXXXXXXXXXXXXXXXXVLPSLDWADFSDP-TL 64

Query: 69  YRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGRITAEEL 128
            RKEL+ALLGLIGAEPPSEEE+K+MM ++D+VGP CHSELRDTFEIFDADHDGRITAEEL
Sbjct: 65  SRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITAEEL 124

Query: 129 FSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 173
           FSVF+AMGDE CTLEDCRRMIAGVDKNGDGFVCF+DFA MM+CQR
Sbjct: 125 FSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

[Close]

BLAST of Carg09817 vs. TrEMBL
Match: tr|A0A2P6RTD5|A0A2P6RTD5_ROSCH (Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0124821 PE=4 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.0e-47
Identity = 121/226 (53.54%), Postives = 144/226 (63.72%), Query Frame = 0


Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSS------------------- 60
           MKL KI   S SP RLF S K R+++S+S+PPSFGS ++SS                   
Sbjct: 1   MKLMKI--NSLSPKRLFRSMKDRSAVSRSDPPSFGSGTASSSSSDPLPSDHKPGPGGMVT 60

Query: 61  PTSVLPPHXXXXLSGEWSDFS-DTHTDY----------------RKELEALLGLIGAEPP 120
           PTSVLP      +SG+WSDFS DTH D                 RKELEALL  +GAEPP
Sbjct: 61  PTSVLP-----EISGDWSDFSADTHLDMAQAFKLIDRDNDGVVSRKELEALLKRLGAEPP 120

Query: 121 SEEELKMMMSQVD--------------KVGPPCH----SELRDTFEIFDADHDGRITAEE 173
           S EE+ MM+S+VD              + GP C     SELR+ FE+FDADHDG+I+AEE
Sbjct: 121 SHEEVAMMLSEVDRDGDGCIPLEALLHRFGPACEPAADSELREAFEVFDADHDGKISAEE 180

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BLAST of Carg09817 vs. TrEMBL
Match: tr|A0A2I4ECU2|A0A2I4ECU2_9ROSI (probable calcium-binding protein CML36 OS=Juglans regia OX=51240 GN=LOC108988409 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.5e-43
Identity = 116/235 (49.36%), Postives = 136/235 (57.87%), Query Frame = 0


Query: 1   MKLPKITAKS-----FSPGRLFPSKKHRTSLSKSEPPSFGS------------------- 60
           MKL KI   +      SP RLF SKK R+S+S+S+P SFGS                   
Sbjct: 1   MKLVKINQLNPKNLIVSPTRLFRSKKDRSSVSRSDPSSFGSXXXXXXXXXXXXSHQKPGS 60

Query: 61  ----ASSSSPTSVLPPHXXXXLSGEWSDFS-----------------DTHTDYRKELEAL 120
                +  +P SVLP      + G WSD S                 D     RKEL+AL
Sbjct: 61  GVGITNLGTPKSVLP-----EILGNWSDSSADMNVELAQAFRLTDRDDDGLVSRKELQAL 120

Query: 121 LGLIGAEPPSEEELKMMMSQVDK------------------VGPPCHSELRDTFEIFDAD 173
           L  IGAEP SEEE+KMM+++VD+                   GP C SELR+TF+IFDAD
Sbjct: 121 LSRIGAEPLSEEEVKMMLNEVDRDGDGHISLETLLSRFGSACGPACDSELRETFDIFDAD 180

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BLAST of Carg09817 vs. TrEMBL
Match: tr|M5WA96|M5WA96_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G047300 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 5.0e-42
Identity = 114/234 (48.72%), Postives = 141/234 (60.26%), Query Frame = 0


Query: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSK--SEPPSFGSASS------------------- 60
           MKL KI++KS SP RLF SKK R+++S+  S+P SF S +                    
Sbjct: 1   MKLIKISSKSLSPKRLFRSKKDRSAVSRRSSDPLSFESGTXXXXXXXXXXXXXLHKPGTG 60

Query: 61  ------SSPTSVLPPHXXXXLSGEWSDFS-DTHTDY----------------RKELEALL 120
                  +PTSVL        SG+WSDFS D   D                 RKELEALL
Sbjct: 61  AEAGGIGTPTSVL-----LERSGDWSDFSTDLQLDMAQAFKLIDRDNDGVVSRKELEALL 120

Query: 121 GLIGAEPPSEEELKMMMSQVD--------------KVGPPC----HSELRDTFEIFDADH 173
             +GA+PPS+EE+ +M+S+VD              +VGP C     SELRD FE+FD+DH
Sbjct: 121 SRLGADPPSQEEVMLMLSEVDREGNGSISLEALLSRVGPVCGPAADSELRDAFEVFDSDH 180

The following BLAST results are available for this feature:

BLAST of Carg09817 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022935658.1	8.8e-91	99.42	probable calcium-binding protein CML36 [Cucurbita moschata]	[more]
XP_022983878.1	1.1e-88	95.95	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]
XP_023527899.1	3.2e-85	95.38	probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo]	[more]
XP_004136671.2	3.7e-57	70.18	PREDICTED: probable calcium-binding protein CML35 [Cucumis sativus] >KGN59507.1 ...	[more]
XP_008443339.2	3.2e-56	72.73	PREDICTED: probable calcium-binding protein CML35 [Cucumis melo]	[more]

BLAST of Carg09817 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G41410.1	2.4e-21	35.86	Calcium-binding EF-hand family protein	[more]
AT3G10190.1	5.2e-21	35.51	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	5.1e-16	38.14	EF hand calcium-binding protein family	[more]
AT1G18210.1	3.8e-11	48.44	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	4.9e-11	51.56	EF hand calcium-binding protein family	[more]

BLAST of Carg09817 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P30188\|CML35_ARATH	4.2e-20	35.86	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
sp\|Q9SS31\|CML36_ARATH	9.4e-20	35.51	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
sp\|Q9FYK2\|CML25_ARATH	9.1e-15	38.14	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
sp\|Q5ZCK5\|CML16_ORYSJ	2.8e-11	54.69	Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q6L4D4\|CML15_ORYSJ	4.0e-10	52.38	Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Carg09817 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LFY7\|A0A0A0LFY7_CUCSA	2.5e-57	70.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823060 PE=4 SV=1	[more]
tr\|A0A1S3B7R8\|A0A1S3B7R8_CUCME	2.1e-56	72.73	probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 P...	[more]
tr\|A0A2P6RTD5\|A0A2P6RTD5_ROSCH	1.0e-47	53.54	Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2...	[more]
tr\|A0A2I4ECU2\|A0A2I4ECU2_9ROSI	3.5e-43	49.36	probable calcium-binding protein CML36 OS=Juglans regia OX=51240 GN=LOC108988409...	[more]
tr\|M5WA96\|M5WA96_PRUPE	5.0e-42	48.72	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G047300 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09817-RA	Carg09817-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 143..171 e-value: 0.0017 score: 27.6 coord: 106..134 e-value: 3.1E-5 score: 33.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 106..169 e-value: 2.8E-9 score: 37.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 102..137 score: 12.923
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 139..173 score: 9.604
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 106..168 e-value: 4.98552E-18 score: 73.3509
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 103..135 e-value: 6.4E-9 score: 37.1
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 136..169 e-value: 7.1E-9 score: 37.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..62
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..44
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 100..172
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 69..101
None	No IPR available	PANTHER	PTHR10891:SF739	CALCIUM-BINDING PROTEIN CML36-RELATED	coord: 100..172 coord: 69..101
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 152..164
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 115..127
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 70..168

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg09817	Cla016954	Watermelon (97103) v1	carwmB0431
Carg09817	Csa3G823060	Cucumber (Chinese Long) v2	carcuB0467
Carg09817	MELO3C009818	Melon (DHL92) v3.5.1	carmeB0443
Carg09817	ClCG10G015590	Watermelon (Charleston Gray)	carwcgB0426
Carg09817	CSPI03G38420	Wild cucumber (PI 183967)	carcpiB0472
Carg09817	Cucsa.338310	Cucumber (Gy14) v1	carcgyB0506
Carg09817	CmaCh14G005570	Cucurbita maxima (Rimu)	carcmaB0607
Carg09817	CmoCh14G005610	Cucurbita moschata (Rifu)	carcmoB0592
Carg09817	Lsi03G015390	Bottle gourd (USVL1VR-Ls)	carlsiB394
Carg09817	Cp4.1LG03g08270	Cucurbita pepo (Zucchini)	carcpeB0613
Carg09817	CsGy3G035950	Cucumber (Gy14) v2	carcgybB0444
Carg09817	MELO3C009818.2	Melon (DHL92) v3.6.1	carmedB0445
Carg09817	CsaV3_3G040820	Cucumber (Chinese Long) v3	carcucB0468
Carg09817	Cla97C10G198500	Watermelon (97103) v2	carwmbB0456
Carg09817	Bhi11G002433	Wax gourd	carwgoB0521

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg09817	Wax gourd	carwgoB0522