CmoCh03G008250 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh03G008250
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	RING/U-box superfamily protein
Location	Cmo_Chr03 : 6606729 .. 6607425 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTTGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATTGTTGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTGATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGTTTTTCTAGGGCGAAATACCAATTTCATTTTGTTCATCACAGAATAGAATCAAATACCATAGAATCTTGATTTTCTGTATTAACTAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGA

mRNA sequence

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTTGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATTGTTGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTGATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGA

Coding sequence (CDS)

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTTGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATTGTTGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTGATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGA

Homology

[Close]

BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: RHA2A_ARATH (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana GN=RHA2A PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-13
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 1


Query: 75  CAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRR 132
           C VCLS+ +EGE VRKL+C H FHK CL+ WL  F  TCPLCR  ++ DD V+  +R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

[Close]

BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: XERIC_ARATH (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana GN=XERICO PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.4e-13
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 1


Query: 41  DKRVITTAQFLKLIEEKNPTIRFSK--KVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFH 100
           D RV     +L+    + PT+RF    +    +D++C+VCLS+F+    + KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 101 KDCLDNWLKLFFATCPLCRCK--VLPDD 125
           K CL+ W+  +  TCPLCR    V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

[Close]

BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: RHA2B_ARATH (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana GN=RHA2B PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 9.8e-13
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = 1


Query: 21  LLLLECVTLIRTIFGLR--PNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVC 80
           +LL    T  R +  L   P++   V+ T+    L ++ N    FS + + ++   C VC
Sbjct: 18  MLLALLATFFRHVRSLLLFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVC 77

Query: 81  LSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLP 123
           LS+ + GE VRKL C H FHK CL+ WL+     CPLCR  +LP
Sbjct: 78  LSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.4e-12
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 1


Query: 59  PTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 118
           PT++ +K +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 119 KVLPDD 125
           ++  DD
Sbjct: 260 ELPTDD 264

[Close]

BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: RN128_XENLA (E3 ubiquitin-protein ligase RNF128 OS=Xenopus laevis GN=rnf128 PE=2 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 5.4e-11
Identity = 29/62 (46.77%), Postives = 40/62 (64.52%), Query Frame = 1


Query: 60  TIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 119
           TI+   KV     D CAVC+  ++  ++VR L CNH FHK+C+D WL L   TCP+C+C 
Sbjct: 245 TIKQGDKVLGPDGDSCAVCIEPYKPSDVVRILTCNHFFHKNCIDPWL-LEHRTCPMCKCD 304

Query: 120 VL 122
           +L
Sbjct: 305 IL 305

[Close]

BLAST of CmoCh03G008250 vs. TrEMBL
Match: A0A0A0KM08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501220 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 4.4e-76
Identity = 145/159 (91.19%), Postives = 147/159 (92.45%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPN+DKRVITTAQF KLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHGNSIYR
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYR 159

[Close]

BLAST of CmoCh03G008250 vs. TrEMBL
Match: A0A061DI69_THECC (RING-H2 zinc finger protein RHA2a OS=Theobroma cacao GN=TCM_001205 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.0e-56
Identity = 110/162 (67.90%), Postives = 137/162 (84.57%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           M ALSE+FSHL+TMT VF TLLLLE V LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALSEYFSHLYTMTIVFFTLLLLEVVILIRSVTGGVCDSEKRLITTTQYLKFIEEKNPT 60

Query: 61  IRFSKKVTSSSD-DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 120
           +R+S + +S  + ++CAVCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR K
Sbjct: 61  VRYSSRSSSRLESNECAVCLSELEEGEEVRKLKCKHTFHKDCLDRWLQQYWATCPLCRTK 120

Query: 121 VLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYRKY 162
           VLPD+IVA Y RL++RVEYDGSDEE+IFLLSALHGNS++R +
Sbjct: 121 VLPDEIVANYHRLQNRVEYDGSDEEMIFLLSALHGNSLHRLF 162

[Close]

BLAST of CmoCh03G008250 vs. TrEMBL
Match: M5WD85_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012579mg PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.1e-54
Identity = 102/161 (63.35%), Postives = 136/161 (84.47%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALS+FFSHL+T+T +F TLL+LE V L+R+I GL PN+ KRVITT Q+LKLIE+KNPT
Sbjct: 1   MGALSQFFSHLYTITLIFFTLLILEIVILVRSIAGLNPNSSKRVITTTQYLKLIEQKNPT 60

Query: 61  IRFSKKVTSSSDD--QCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 120
             +++K+ S   +  +C+VCLS+FEEG+ +R+L+C HTFH++CLD WL+ ++ATCPLCR 
Sbjct: 61  TCYTQKLRSVRPEATECSVCLSDFEEGDKIRQLKCKHTFHQNCLDRWLQQYWATCPLCRT 120

Query: 121 KVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           KVL DD+VA Y RLR++V+YDGSDEELIFLLS+L GNS++R
Sbjct: 121 KVLSDDVVASYHRLRNQVQYDGSDEELIFLLSSLQGNSLHR 161

[Close]

BLAST of CmoCh03G008250 vs. TrEMBL
Match: A0A0D2QDA2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G045800 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 3.6e-54
Identity = 106/164 (64.63%), Postives = 133/164 (81.10%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           M AL+EFFSHL+TMT VF TLLLLE   LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALTEFFSHLYTMTVVFFTLLLLEVAILIRSLTGGLSDSEKRLITTTQYLKFIEEKNPT 60

Query: 61  IRFSKKVTSSSD---DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCR 120
           I +S + +S  D   ++C VCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR
Sbjct: 61  IIYSTRSSSRVDLSSNECTVCLSELEEGEKVRKLKCKHTFHKDCLDRWLQQYWATCPLCR 120

Query: 121 CKVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYRKY 162
            KVLPD++VA Y RL+++VEYDGSDEE+IFLLSALH NS++R +
Sbjct: 121 TKVLPDEVVANYHRLQNQVEYDGSDEEMIFLLSALHDNSLHRLF 164

[Close]

BLAST of CmoCh03G008250 vs. TrEMBL
Match: B9H581_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08300g PE=4 SV=2)

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-52
Identity = 102/160 (63.75%), Postives = 128/160 (80.00%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           M +LSEFFSHL+TM  VF++LLLLE V LIR++ G    +DK +I+T Q+L+ IEEKNPT
Sbjct: 1   MASLSEFFSHLYTMAIVFLSLLLLEIVILIRSVIGSTLKSDKPIISTTQYLRHIEEKNPT 60

Query: 61  IRFSKKVTSSSDD-QCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 120
           I +SK++    D  +CAVCLSEF EGE VRKL+C HTFHKDCLD WL+   ATCPLCR K
Sbjct: 61  ISYSKQLMRQQDSIECAVCLSEFSEGESVRKLKCKHTFHKDCLDEWLQQCLATCPLCRAK 120

Query: 121 VLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           VLPD+I+A Y R++ ++EYDGSDEE+IF+LSALHGNS+ R
Sbjct: 121 VLPDEILAKYDRMQSQIEYDGSDEEMIFMLSALHGNSLQR 160

[Close]

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT1G15100.1 (AT1G15100.1 RING-H2 finger A2A)

HSP 1 Score: 77.4 bits (189), Expect = 1.1e-14
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 1


Query: 75  CAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRR 132
           C VCLS+ +EGE VRKL+C H FHK CL+ WL  F  TCPLCR  ++ DD V+  +R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

[Close]

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT2G04240.1 (AT2G04240.1 RING/U-box superfamily protein)

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-14
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 1


Query: 41  DKRVITTAQFLKLIEEKNPTIRFSK--KVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFH 100
           D RV     +L+    + PT+RF    +    +D++C+VCLS+F+    + KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 101 KDCLDNWLKLFFATCPLCRCK--VLPDD 125
           K CL+ W+  +  TCPLCR    V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

[Close]

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT2G01150.1 (AT2G01150.1 RING-H2 finger protein 2B)

HSP 1 Score: 75.1 bits (183), Expect = 5.5e-14
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = 1


Query: 21  LLLLECVTLIRTIFGLR--PNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVC 80
           +LL    T  R +  L   P++   V+ T+    L ++ N    FS + + ++   C VC
Sbjct: 18  MLLALLATFFRHVRSLLLFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVC 77

Query: 81  LSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLP 123
           LS+ + GE VRKL C H FHK CL+ WL+     CPLCR  +LP
Sbjct: 78  LSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 72.4 bits (176), Expect = 3.6e-13
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 1


Query: 59  PTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 118
           PT++ +K +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 119 KVLPDD 125
           ++  DD
Sbjct: 260 ELPTDD 264

[Close]

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT3G61460.1 (AT3G61460.1 brassinosteroid-responsive RING-H2)

HSP 1 Score: 69.3 bits (168), Expect = 3.0e-12
Identity = 34/76 (44.74%), Postives = 45/76 (59.21%), Query Frame = 1


Query: 53  LIEEKNPTIRFSKKVTSSSD--DQCAVCLSEFEEGEMVRKLQ-CNHTFHKDCLDNWLKLF 112
           LI E  P I+F +   S  D  + CAVCL EFE  + +R L+ C H FH+ CLD W+   
Sbjct: 70  LIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRWMDHD 129

Query: 113 FATCPLCRCKVLPDDI 126
             TCPLCR   +PD++
Sbjct: 130 QKTCPLCRTPFVPDEM 145

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|700193577|gb|KGN48781.1| (hypothetical protein Csa_6G501220 [Cucumis sativus])

HSP 1 Score: 292.4 bits (747), Expect = 6.3e-76
Identity = 145/159 (91.19%), Postives = 147/159 (92.45%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPN+DKRVITTAQF KLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHGNSIYR
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYR 159

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|659080170|ref|XP_008440648.1| (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo])

HSP 1 Score: 282.7 bits (722), Expect = 5.0e-73
Identity = 141/153 (92.16%), Postives = 142/153 (92.81%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPNADKRVITTAQFLKLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 154
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALH
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH 153

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|590707663|ref|XP_007048067.1| (RING-H2 zinc finger protein RHA2a [Theobroma cacao])

HSP 1 Score: 228.0 bits (580), Expect = 1.5e-56
Identity = 110/162 (67.90%), Postives = 137/162 (84.57%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           M ALSE+FSHL+TMT VF TLLLLE V LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALSEYFSHLYTMTIVFFTLLLLEVVILIRSVTGGVCDSEKRLITTTQYLKFIEEKNPT 60

Query: 61  IRFSKKVTSSSD-DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 120
           +R+S + +S  + ++CAVCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR K
Sbjct: 61  VRYSSRSSSRLESNECAVCLSELEEGEEVRKLKCKHTFHKDCLDRWLQQYWATCPLCRTK 120

Query: 121 VLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYRKY 162
           VLPD+IVA Y RL++RVEYDGSDEE+IFLLSALHGNS++R +
Sbjct: 121 VLPDEIVANYHRLQNRVEYDGSDEEMIFLLSALHGNSLHRLF 162

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|470132684|ref|XP_004302206.1| (PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Fragaria vesca subsp. vesca])

HSP 1 Score: 222.6 bits (566), Expect = 6.1e-55
Identity = 103/161 (63.98%), Postives = 135/161 (83.85%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALS+FFSHL+T++ +F TL  LE V L+R+I GL+PN++KRVITTAQ+LKLI++KNPT
Sbjct: 1   MGALSQFFSHLYTISIIFFTLFALEVVILVRSITGLKPNSEKRVITTAQYLKLIDQKNPT 60

Query: 61  IRFS--KKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 120
           + +S  KK    + ++C+VCLSEFEEGE +R+L+C H FHKDC+D WL+ ++ATCPLCR 
Sbjct: 61  VSYSSKKKNLLRAHEECSVCLSEFEEGEKIRQLKCKHRFHKDCVDKWLQQYWATCPLCRT 120

Query: 121 KVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           KV+PDD+VA Y RLR++VEYDGSDEELIFLLS+L GN+  R
Sbjct: 121 KVVPDDVVASYHRLRNQVEYDGSDEELIFLLSSLQGNNFQR 161

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|645303061|ref|XP_008245902.1| (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Prunus mume])

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-54
Identity = 103/161 (63.98%), Postives = 136/161 (84.47%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALS+FFSHL+T+T +F TLL+LE V L+R+I GL PN+ KRVITT Q+LKLIE+KNPT
Sbjct: 1   MGALSQFFSHLYTITLIFFTLLILEIVILVRSIAGLNPNSSKRVITTTQYLKLIEQKNPT 60

Query: 61  IRFSKKVTSSSDD--QCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 120
             +++K+ S   +  +C+VCLSEFEEG+ +R+L+C HTFH++CLD WL+ ++ATCPLCR 
Sbjct: 61  TCYTQKLRSVRPEATECSVCLSEFEEGDKIRQLKCKHTFHQNCLDRWLQQYWATCPLCRT 120

Query: 121 KVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           KVL DD+VA Y RLR++V+YDGSDEELIFLLS+L GNS++R
Sbjct: 121 KVLSDDVVASYHRLRNQVQYDGSDEELIFLLSSLQGNSLHR 161

The following BLAST results are available for this feature:

BLAST of CmoCh03G008250 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RHA2A_ARATH	2.0e-13	56.14	E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana GN=RHA2A PE=1 SV=1	[more]
XERIC_ARATH	3.4e-13	38.64	Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana GN=XERICO PE...	[more]
RHA2B_ARATH	9.8e-13	37.50	E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana GN=RHA2B PE=1 SV=2	[more]
RNG1L_ARATH	6.4e-12	45.45	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1...	[more]
RN128_XENLA	5.4e-11	46.77	E3 ubiquitin-protein ligase RNF128 OS=Xenopus laevis GN=rnf128 PE=2 SV=2	[more]

BLAST of CmoCh03G008250 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KM08_CUCSA	4.4e-76	91.19	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501220 PE=4 SV=1	[more]
A0A061DI69_THECC	1.0e-56	67.90	RING-H2 zinc finger protein RHA2a OS=Theobroma cacao GN=TCM_001205 PE=4 SV=1	[more]
M5WD85_PRUPE	2.1e-54	63.35	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012579mg PE=4 SV=1	[more]
A0A0D2QDA2_GOSRA	3.6e-54	64.63	Uncharacterized protein OS=Gossypium raimondii GN=B456_003G045800 PE=4 SV=1	[more]
B9H581_POPTR	1.5e-52	63.75	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08300g PE=4 SV=2	[more]

BLAST of CmoCh03G008250 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G15100.1	1.1e-14	56.14	RING-H2 finger A2A	[more]
AT2G04240.1	1.9e-14	38.64	RING/U-box superfamily protein	[more]
AT2G01150.1	5.5e-14	37.50	RING-H2 finger protein 2B	[more]
AT3G19950.1	3.6e-13	45.45	RING/U-box superfamily protein	[more]
AT3G61460.1	3.0e-12	44.74	brassinosteroid-responsive RING-H2	[more]

BLAST of CmoCh03G008250 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700193577\|gb\|KGN48781.1\|	6.3e-76	91.19	hypothetical protein Csa_6G501220 [Cucumis sativus]	[more]
gi\|659080170\|ref\|XP_008440648.1\|	5.0e-73	92.16	PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo]	[more]
gi\|590707663\|ref\|XP_007048067.1\|	1.5e-56	67.90	RING-H2 zinc finger protein RHA2a [Theobroma cacao]	[more]
gi\|470132684\|ref\|XP_004302206.1\|	6.1e-55	63.98	PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Fragaria vesca subsp. ve...	[more]
gi\|645303061\|ref\|XP_008245902.1\|	1.4e-54	63.98	PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G008250.1	CmoCh03G008250.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 73..117 score: 1.8
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 75..116 score: 2.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 75..117 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 70..120 score: 2.1
None	No IPR available	PANTHER	PTHR14155	RING FINGER DOMAIN-CONTAINING	coord: 59..142 score: 6.7
None	No IPR available	PANTHER	PTHR14155:SF175	E3 UBIQUITIN-PROTEIN LIGASE RHA2B-RELATED	coord: 59..142 score: 6.7
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 64..123 score: 6.48

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh03G008250	Cucsa.104680	Cucumber (Gy14) v1	cgycmoB0227
CmoCh03G008250	CmaCh03G007910	Cucurbita maxima (Rimu)	cmacmoB653
CmoCh03G008250	CmaCh07G005200	Cucurbita maxima (Rimu)	cmacmoB858
CmoCh03G008250	Cla014172	Watermelon (97103) v1	cmowmB623
CmoCh03G008250	Csa6G501220	Cucumber (Chinese Long) v2	cmocuB670
CmoCh03G008250	MELO3C007785	Melon (DHL92) v3.5.1	cmomeB605
CmoCh03G008250	ClCG01G019070	Watermelon (Charleston Gray)	cmowcgB575
CmoCh03G008250	CSPI06G30530	Wild cucumber (PI 183967)	cmocpiB681
CmoCh03G008250	Lsi01G007320	Bottle gourd (USVL1VR-Ls)	cmolsiB582
CmoCh03G008250	Cp4.1LG10g01110	Cucurbita pepo (Zucchini)	cmocpeB606
CmoCh03G008250	Cp4.1LG19g09770	Cucurbita pepo (Zucchini)	cmocpeB624
CmoCh03G008250	MELO3C007785.2	Melon (DHL92) v3.6.1	cmomedB694
CmoCh03G008250	CsaV3_6G045750	Cucumber (Chinese Long) v3	cmocucB0793
CmoCh03G008250	Cla97C01G018530	Watermelon (97103) v2	cmowmbB642
CmoCh03G008250	Bhi03G000946	Wax gourd	cmowgoB0792
CmoCh03G008250	CsGy6G030020	Cucumber (Gy14) v2	cgybcmoB811
CmoCh03G008250	Carg16020	Silver-seed gourd	carcmoB1028
CmoCh03G008250	Carg00676	Silver-seed gourd	carcmoB0551

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmoCh03G008250	CmoCh07G005150	Cucurbita moschata (Rifu)	cmocmoB455

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh03G008250	Cucurbita moschata (Rifu)	cmocmoB451
CmoCh03G008250	Cucurbita maxima (Rimu)	cmacmoB727
CmoCh03G008250	Cucurbita pepo (Zucchini)	cmocpeB613