Cp4.1LG10g01110 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG10g01110
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionE3 ubiquitin-protein ligase RHA2A
LocationCp4.1LG10 : 3386256 .. 3388389 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGCAGGGACGATTTTTTTCCGAAAGATTATAAAATTAAAAGAATTGAATCAGTGGAAACTATCCGCCAATTTCCATCCCGAAGCCATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTCGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATCGTCGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTCATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGTTTTTCTAGGGCGAAATACCAATTTCATTTTGTTCATCACAGAATAGAATCAAATAGCATAGAATCTTGATTTTCTGTATTAACTAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGATACTTCGTTTGCATGATTGCTTCCTACTCTTCCTGGTTATTCGTCAACGAATATCATTAATTCTGGGGACAGCCAATTTACTTGATTTTTTTTNGGGGGACCTTTGTTCCCAATAAGACACTGAAATCTGCTCTAGAATCATCCAAAGCATAGCATCTTTTATCCATCCGGAAGTTTACTTGGGCTAATCCGAAGGATCTTTAACAAAATTGAAGATGTTAGTTAGATGATCCCTTAGTGTCATTTGAAGGAGGCTTATTGCCTCGTAGGGCCAGGGGATATTATGCGACTTATAATTATAGTAGATGCGAGTTGGTGGTCTAATTTATGGGGCTTTTAGAGGGTAACTAGAATGGTACTTGACGGCATTTATTAAGAACATAAGTTGAAAGAAAAAGCAAAATTTGTTGGTTTTCCCTTGTGTTTCCATGGGGGTTTAGGTGGAGATGGTAGGAGGAGGACCAAAGGCTCTGTTTGTAGGCTGTAACAAACTGATTTAGCTCATCTTCTCTGTAGTTAAGCCCCAATCTTATGTGTTTCTGTTGCCCATTTTCTTCAATCCTACTCCAACTCTTGAACACCCATCAAAAGATGAGGGAAATTGAGGACATGCTTAATTAGCAGCCTGGTCTGATAAAACAATTTTCAAGAACCATGATTGGGTCAGGTGACTTGTATTATTAAGGTTATATTGCGTAGTTCAAGGAATAACAACTTTAGCATTCTCAGAATCAAGTTTTATATGGTGAACCCAAATACCTTCCCACTGTCTTTAGGACTGTTCCTGAGAATAAGTTCGAAAAATTTCCTCTCCTAAATATTCTGTACGTCGGGCACTTGCAATAAGTTGAAAGGGAGCAGGCTAGACCTGGTGGTGCAGGGAAAGGTAATTGTAAAAGATTTGACATTTAATACCAGATTGTTGAGTAATAGTACCAAACCAAAAGAGCGCATCTGTTCTGGCCGTGAGTTTGGTTAGATGCAACTGATCCCATTTGAAAATTAGCGGTAATCAGGGCTGCTGGACTTTAGCATTATGTTCTTGGTGCTTTGAATTATACTCGTTTATTTTGAGATTAATTTGTGTTAGATTATCTGATGAAGATAGAATTGATTTGATGATTTGATGATTTGATTGATAGCTGTTATATCCGTTGGATTTTAAAATCATAAGGAATCTTTTATAACATGCCTATCATTCTATGTTGTGACAGCTGCCGGCAACAATTATCTGTAAGCTTGGTTTGGGGCCGAAAATAAGGGACATTCTGGATGGTCTAACATTTATATGATTATACAGTTCATGAATATAAAGATTAACCTTTATTTTTTTGTACAGTAAATAACTGA

mRNA sequence

AATGCAGGGACGATTTTTTTCCGAAAGATTATAAAATTAAAAGAATTGAATCAGTGGAAACTATCCGCCAATTTCCATCCCGAAGCCATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTCGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATCGTCGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTCATCTTTTTGTTATCGGCATTACATGTAAATAACTGA

Coding sequence (CDS)

AATGCAGGGACGATTTTTTTCCGAAAGATTATAAAATTAAAAGAATTGAATCAGTGGAAACTATCCGCCAATTTCCATCCCGAAGCCATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTCGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATCGTCGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTCATCTTTTTGTTATCGGCATTACATGTAAATAACTGA

Protein sequence

NAGTIFFRKIIKLKELNQWKLSANFHPEAMGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN
BLAST of Cp4.1LG10g01110 vs. Swiss-Prot
Match: RHA2A_ARATH (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana GN=RHA2A PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-13
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 1

Query: 104 CAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRR 161
           C VCLS+ +EGE VRKL+C H FHK CL+ WL  F  TCPLCR  ++ DD V+  +R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

BLAST of Cp4.1LG10g01110 vs. Swiss-Prot
Match: XERIC_ARATH (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana GN=XERICO PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.1e-13
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 1

Query: 70  DKRVITTAQFLKLIEEKNPTIRFSK--KVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFH 129
           D RV     +L+    + PT+RF    +    +D++C+VCLS+F+    + KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 130 KDCLDNWLKLFFATCPLCRCK--VLPDD 154
           K CL+ W+  +  TCPLCR    V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

BLAST of Cp4.1LG10g01110 vs. Swiss-Prot
Match: RHA2B_ARATH (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana GN=RHA2B PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 9.0e-13
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = 1

Query: 50  LLLLECVTLIRTIFGLR--PNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVC 109
           +LL    T  R +  L   P++   V+ T+    L ++ N    FS + + ++   C VC
Sbjct: 18  MLLALLATFFRHVRSLLLFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVC 77

Query: 110 LSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLP 152
           LS+ + GE VRKL C H FHK CL+ WL+     CPLCR  +LP
Sbjct: 78  LSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

BLAST of Cp4.1LG10g01110 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.8e-12
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 1

Query: 88  PTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 147
           PT++ +K +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 148 KVLPDD 154
           ++  DD
Sbjct: 260 ELPTDD 264

BLAST of Cp4.1LG10g01110 vs. Swiss-Prot
Match: RN128_XENLA (E3 ubiquitin-protein ligase RNF128 OS=Xenopus laevis GN=rnf128 PE=2 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 4.9e-11
Identity = 29/62 (46.77%), Postives = 40/62 (64.52%), Query Frame = 1

Query: 89  TIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 148
           TI+   KV     D CAVC+  ++  ++VR L CNH FHK+C+D WL L   TCP+C+C 
Sbjct: 245 TIKQGDKVLGPDGDSCAVCIEPYKPSDVVRILTCNHFFHKNCIDPWL-LEHRTCPMCKCD 304

Query: 149 VL 151
           +L
Sbjct: 305 IL 305

BLAST of Cp4.1LG10g01110 vs. TrEMBL
Match: A0A0A0KM08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501220 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.1e-73
Identity = 140/156 (89.74%), Postives = 143/156 (91.67%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPN+DKRVITTAQF KLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 90  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 149
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 150 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN 186
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALH N+
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNS 156

BLAST of Cp4.1LG10g01110 vs. TrEMBL
Match: A0A061DI69_THECC (RING-H2 zinc finger protein RHA2a OS=Theobroma cacao GN=TCM_001205 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.9e-54
Identity = 107/157 (68.15%), Postives = 132/157 (84.08%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           M ALSE+FSHL+TMT VF TLLLLE V LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALSEYFSHLYTMTIVFFTLLLLEVVILIRSVTGGVCDSEKRLITTTQYLKFIEEKNPT 60

Query: 90  IRFSKKVTSSSD-DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 149
           +R+S + +S  + ++CAVCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR K
Sbjct: 61  VRYSSRSSSRLESNECAVCLSELEEGEEVRKLKCKHTFHKDCLDRWLQQYWATCPLCRTK 120

Query: 150 VLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN 186
           VLPD+IVA Y RL++RVEYDGSDEE+IFLLSALH N+
Sbjct: 121 VLPDEIVANYHRLQNRVEYDGSDEEMIFLLSALHGNS 157

BLAST of Cp4.1LG10g01110 vs. TrEMBL
Match: A0A0D2QDA2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G045800 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.1e-52
Identity = 104/159 (65.41%), Postives = 129/159 (81.13%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           M AL+EFFSHL+TMT VF TLLLLE   LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALTEFFSHLYTMTVVFFTLLLLEVAILIRSLTGGLSDSEKRLITTTQYLKFIEEKNPT 60

Query: 90  IRFSKKVTSSSD---DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCR 149
           I +S + +S  D   ++C VCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR
Sbjct: 61  IIYSTRSSSRVDLSSNECTVCLSELEEGEKVRKLKCKHTFHKDCLDRWLQQYWATCPLCR 120

Query: 150 CKVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN 186
            KVLPD++VA Y RL+++VEYDGSDEE+IFLLSALH N+
Sbjct: 121 TKVLPDEVVANYHRLQNQVEYDGSDEEMIFLLSALHDNS 159

BLAST of Cp4.1LG10g01110 vs. TrEMBL
Match: M5WD85_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012579mg PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 3.1e-52
Identity = 99/158 (62.66%), Postives = 132/158 (83.54%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           MGALS+FFSHL+T+T +F TLL+LE V L+R+I GL PN+ KRVITT Q+LKLIE+KNPT
Sbjct: 1   MGALSQFFSHLYTITLIFFTLLILEIVILVRSIAGLNPNSSKRVITTTQYLKLIEQKNPT 60

Query: 90  IRFSKKVTSSSDD--QCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 149
             +++K+ S   +  +C+VCLS+FEEG+ +R+L+C HTFH++CLD WL+ ++ATCPLCR 
Sbjct: 61  TCYTQKLRSVRPEATECSVCLSDFEEGDKIRQLKCKHTFHQNCLDRWLQQYWATCPLCRT 120

Query: 150 KVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN 186
           KVL DD+VA Y RLR++V+YDGSDEELIFLLS+L  N+
Sbjct: 121 KVLSDDVVASYHRLRNQVQYDGSDEELIFLLSSLQGNS 158

BLAST of Cp4.1LG10g01110 vs. TrEMBL
Match: B9H581_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08300g PE=4 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 9.9e-51
Identity = 99/157 (63.06%), Postives = 125/157 (79.62%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           M +LSEFFSHL+TM  VF++LLLLE V LIR++ G    +DK +I+T Q+L+ IEEKNPT
Sbjct: 1   MASLSEFFSHLYTMAIVFLSLLLLEIVILIRSVIGSTLKSDKPIISTTQYLRHIEEKNPT 60

Query: 90  IRFSKKVTSSSDD-QCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 149
           I +SK++    D  +CAVCLSEF EGE VRKL+C HTFHKDCLD WL+   ATCPLCR K
Sbjct: 61  ISYSKQLMRQQDSIECAVCLSEFSEGESVRKLKCKHTFHKDCLDEWLQQCLATCPLCRAK 120

Query: 150 VLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN 186
           VLPD+I+A Y R++ ++EYDGSDEE+IF+LSALH N+
Sbjct: 121 VLPDEILAKYDRMQSQIEYDGSDEEMIFMLSALHGNS 157

BLAST of Cp4.1LG10g01110 vs. TAIR10
Match: AT1G15100.1 (AT1G15100.1 RING-H2 finger A2A)

HSP 1 Score: 77.4 bits (189), Expect = 1.0e-14
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 1

Query: 104 CAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRR 161
           C VCLS+ +EGE VRKL+C H FHK CL+ WL  F  TCPLCR  ++ DD V+  +R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

BLAST of Cp4.1LG10g01110 vs. TAIR10
Match: AT2G04240.1 (AT2G04240.1 RING/U-box superfamily protein)

HSP 1 Score: 76.6 bits (187), Expect = 1.7e-14
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 1

Query: 70  DKRVITTAQFLKLIEEKNPTIRFSK--KVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFH 129
           D RV     +L+    + PT+RF    +    +D++C+VCLS+F+    + KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 130 KDCLDNWLKLFFATCPLCRCK--VLPDD 154
           K CL+ W+  +  TCPLCR    V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

BLAST of Cp4.1LG10g01110 vs. TAIR10
Match: AT2G01150.1 (AT2G01150.1 RING-H2 finger protein 2B)

HSP 1 Score: 75.1 bits (183), Expect = 5.1e-14
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = 1

Query: 50  LLLLECVTLIRTIFGLR--PNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVC 109
           +LL    T  R +  L   P++   V+ T+    L ++ N    FS + + ++   C VC
Sbjct: 18  MLLALLATFFRHVRSLLLFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVC 77

Query: 110 LSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLP 152
           LS+ + GE VRKL C H FHK CL+ WL+     CPLCR  +LP
Sbjct: 78  LSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

BLAST of Cp4.1LG10g01110 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 72.4 bits (176), Expect = 3.3e-13
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 1

Query: 88  PTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 147
           PT++ +K +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 148 KVLPDD 154
           ++  DD
Sbjct: 260 ELPTDD 264

BLAST of Cp4.1LG10g01110 vs. TAIR10
Match: AT3G61460.1 (AT3G61460.1 brassinosteroid-responsive RING-H2)

HSP 1 Score: 69.3 bits (168), Expect = 2.8e-12
Identity = 34/76 (44.74%), Postives = 45/76 (59.21%), Query Frame = 1

Query: 82  LIEEKNPTIRFSKKVTSSSD--DQCAVCLSEFEEGEMVRKLQ-CNHTFHKDCLDNWLKLF 141
           LI E  P I+F +   S  D  + CAVCL EFE  + +R L+ C H FH+ CLD W+   
Sbjct: 70  LIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRWMDHD 129

Query: 142 FATCPLCRCKVLPDDI 155
             TCPLCR   +PD++
Sbjct: 130 QKTCPLCRTPFVPDEM 145

BLAST of Cp4.1LG10g01110 vs. NCBI nr
Match: gi|659080170|ref|XP_008440648.1| (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo])

HSP 1 Score: 285.0 bits (728), Expect = 9.2e-74
Identity = 142/154 (92.21%), Postives = 143/154 (92.86%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPNADKRVITTAQFLKLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 90  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 149
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 150 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHV 184
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHV
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHV 154

BLAST of Cp4.1LG10g01110 vs. NCBI nr
Match: gi|700193577|gb|KGN48781.1| (hypothetical protein Csa_6G501220 [Cucumis sativus])

HSP 1 Score: 282.3 bits (721), Expect = 5.9e-73
Identity = 140/156 (89.74%), Postives = 143/156 (91.67%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPN+DKRVITTAQF KLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 90  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 149
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 150 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN 186
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALH N+
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNS 156

BLAST of Cp4.1LG10g01110 vs. NCBI nr
Match: gi|470132684|ref|XP_004302206.1| (PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Fragaria vesca subsp. vesca])

HSP 1 Score: 220.3 bits (560), Expect = 2.8e-54
Identity = 102/158 (64.56%), Postives = 133/158 (84.18%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           MGALS+FFSHL+T++ +F TL  LE V L+R+I GL+PN++KRVITTAQ+LKLI++KNPT
Sbjct: 1   MGALSQFFSHLYTISIIFFTLFALEVVILVRSITGLKPNSEKRVITTAQYLKLIDQKNPT 60

Query: 90  IRFS--KKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 149
           + +S  KK    + ++C+VCLSEFEEGE +R+L+C H FHKDC+D WL+ ++ATCPLCR 
Sbjct: 61  VSYSSKKKNLLRAHEECSVCLSEFEEGEKIRQLKCKHRFHKDCVDKWLQQYWATCPLCRT 120

Query: 150 KVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN 186
           KV+PDD+VA Y RLR++VEYDGSDEELIFLLS+L  NN
Sbjct: 121 KVVPDDVVASYHRLRNQVEYDGSDEELIFLLSSLQGNN 158

BLAST of Cp4.1LG10g01110 vs. NCBI nr
Match: gi|590707663|ref|XP_007048067.1| (RING-H2 zinc finger protein RHA2a [Theobroma cacao])

HSP 1 Score: 220.3 bits (560), Expect = 2.8e-54
Identity = 107/157 (68.15%), Postives = 132/157 (84.08%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           M ALSE+FSHL+TMT VF TLLLLE V LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALSEYFSHLYTMTIVFFTLLLLEVVILIRSVTGGVCDSEKRLITTTQYLKFIEEKNPT 60

Query: 90  IRFSKKVTSSSD-DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 149
           +R+S + +S  + ++CAVCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR K
Sbjct: 61  VRYSSRSSSRLESNECAVCLSELEEGEEVRKLKCKHTFHKDCLDRWLQQYWATCPLCRTK 120

Query: 150 VLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN 186
           VLPD+IVA Y RL++RVEYDGSDEE+IFLLSALH N+
Sbjct: 121 VLPDEIVANYHRLQNRVEYDGSDEEMIFLLSALHGNS 157

BLAST of Cp4.1LG10g01110 vs. NCBI nr
Match: gi|823140038|ref|XP_012469879.1| (PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Gossypium raimondii])

HSP 1 Score: 214.5 bits (545), Expect = 1.5e-52
Identity = 104/159 (65.41%), Postives = 129/159 (81.13%), Query Frame = 1

Query: 30  MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 89
           M AL+EFFSHL+TMT VF TLLLLE   LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALTEFFSHLYTMTVVFFTLLLLEVAILIRSLTGGLSDSEKRLITTTQYLKFIEEKNPT 60

Query: 90  IRFSKKVTSSSD---DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCR 149
           I +S + +S  D   ++C VCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR
Sbjct: 61  IIYSTRSSSRVDLSSNECTVCLSELEEGEKVRKLKCKHTFHKDCLDRWLQQYWATCPLCR 120

Query: 150 CKVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHVNN 186
            KVLPD++VA Y RL+++VEYDGSDEE+IFLLSALH N+
Sbjct: 121 TKVLPDEVVANYHRLQNQVEYDGSDEEMIFLLSALHDNS 159

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RHA2A_ARATH1.8e-1356.14E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana GN=RHA2A PE=1 SV=1[more]
XERIC_ARATH3.1e-1338.64Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana GN=XERICO PE... [more]
RHA2B_ARATH9.0e-1337.50E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana GN=RHA2B PE=1 SV=2[more]
RNG1L_ARATH5.8e-1245.45E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1... [more]
RN128_XENLA4.9e-1146.77E3 ubiquitin-protein ligase RNF128 OS=Xenopus laevis GN=rnf128 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KM08_CUCSA4.1e-7389.74Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501220 PE=4 SV=1[more]
A0A061DI69_THECC1.9e-5468.15RING-H2 zinc finger protein RHA2a OS=Theobroma cacao GN=TCM_001205 PE=4 SV=1[more]
A0A0D2QDA2_GOSRA1.1e-5265.41Uncharacterized protein OS=Gossypium raimondii GN=B456_003G045800 PE=4 SV=1[more]
M5WD85_PRUPE3.1e-5262.66Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012579mg PE=4 SV=1[more]
B9H581_POPTR9.9e-5163.06Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08300g PE=4 SV=2[more]
Match NameE-valueIdentityDescription
AT1G15100.11.0e-1456.14 RING-H2 finger A2A[more]
AT2G04240.11.7e-1438.64 RING/U-box superfamily protein[more]
AT2G01150.15.1e-1437.50 RING-H2 finger protein 2B[more]
AT3G19950.13.3e-1345.45 RING/U-box superfamily protein[more]
AT3G61460.12.8e-1244.74 brassinosteroid-responsive RING-H2[more]
Match NameE-valueIdentityDescription
gi|659080170|ref|XP_008440648.1|9.2e-7492.21PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo][more]
gi|700193577|gb|KGN48781.1|5.9e-7389.74hypothetical protein Csa_6G501220 [Cucumis sativus][more]
gi|470132684|ref|XP_004302206.1|2.8e-5464.56PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Fragaria vesca subsp. ve... [more]
gi|590707663|ref|XP_007048067.1|2.8e-5468.15RING-H2 zinc finger protein RHA2a [Theobroma cacao][more]
gi|823140038|ref|XP_012469879.1|1.5e-5265.41PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Gossypium raimondii][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g01110.1Cp4.1LG10g01110.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 102..146
score: 1.6
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 104..145
score: 2.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 104..146
score: 12
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 99..149
score: 1.8
NoneNo IPR availablePANTHERPTHR14155RING FINGER DOMAIN-CONTAININGcoord: 88..171
score: 6.7
NoneNo IPR availablePANTHERPTHR14155:SF175E3 UBIQUITIN-PROTEIN LIGASE RHA2B-RELATEDcoord: 88..171
score: 6.7
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 93..152
score: 1.18

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG10g01110Cp4.1LG19g09770Cucurbita pepo (Zucchini)cpecpeB076
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG10g01110Cucurbita pepo (Zucchini)cpecpeB065
Cp4.1LG10g01110Cucurbita pepo (Zucchini)cpecpeB073
Cp4.1LG10g01110Cucurbita maxima (Rimu)cmacpeB702
Cp4.1LG10g01110Cucurbita moschata (Rifu)cmocpeB655
Cp4.1LG10g01110Melon (DHL92) v3.6.1cpemedB079
Cp4.1LG10g01110Silver-seed gourdcarcpeB1300
Cp4.1LG10g01110Silver-seed gourdcarcpeB1413