CmoCh03G008250 (gene) Cucurbita moschata (Rifu)

NameCmoCh03G008250
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionRING/U-box superfamily protein
LocationCmo_Chr03 : 6606729 .. 6607425 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTTGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATTGTTGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTGATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGTTTTTCTAGGGCGAAATACCAATTTCATTTTGTTCATCACAGAATAGAATCAAATACCATAGAATCTTGATTTTCTGTATTAACTAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGA

mRNA sequence

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTTGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATTGTTGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTGATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGA

Coding sequence (CDS)

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTTGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATTGTTGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTGATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGA
BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: RHA2A_ARATH (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana GN=RHA2A PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-13
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 1

Query: 75  CAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRR 132
           C VCLS+ +EGE VRKL+C H FHK CL+ WL  F  TCPLCR  ++ DD V+  +R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: XERIC_ARATH (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana GN=XERICO PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.4e-13
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 1

Query: 41  DKRVITTAQFLKLIEEKNPTIRFSK--KVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFH 100
           D RV     +L+    + PT+RF    +    +D++C+VCLS+F+    + KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 101 KDCLDNWLKLFFATCPLCRCK--VLPDD 125
           K CL+ W+  +  TCPLCR    V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: RHA2B_ARATH (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana GN=RHA2B PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 9.8e-13
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = 1

Query: 21  LLLLECVTLIRTIFGLR--PNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVC 80
           +LL    T  R +  L   P++   V+ T+    L ++ N    FS + + ++   C VC
Sbjct: 18  MLLALLATFFRHVRSLLLFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVC 77

Query: 81  LSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLP 123
           LS+ + GE VRKL C H FHK CL+ WL+     CPLCR  +LP
Sbjct: 78  LSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.4e-12
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 1

Query: 59  PTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 118
           PT++ +K +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 119 KVLPDD 125
           ++  DD
Sbjct: 260 ELPTDD 264

BLAST of CmoCh03G008250 vs. Swiss-Prot
Match: RN128_XENLA (E3 ubiquitin-protein ligase RNF128 OS=Xenopus laevis GN=rnf128 PE=2 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 5.4e-11
Identity = 29/62 (46.77%), Postives = 40/62 (64.52%), Query Frame = 1

Query: 60  TIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 119
           TI+   KV     D CAVC+  ++  ++VR L CNH FHK+C+D WL L   TCP+C+C 
Sbjct: 245 TIKQGDKVLGPDGDSCAVCIEPYKPSDVVRILTCNHFFHKNCIDPWL-LEHRTCPMCKCD 304

Query: 120 VL 122
           +L
Sbjct: 305 IL 305

BLAST of CmoCh03G008250 vs. TrEMBL
Match: A0A0A0KM08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501220 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 4.4e-76
Identity = 145/159 (91.19%), Postives = 147/159 (92.45%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPN+DKRVITTAQF KLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHGNSIYR
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYR 159

BLAST of CmoCh03G008250 vs. TrEMBL
Match: A0A061DI69_THECC (RING-H2 zinc finger protein RHA2a OS=Theobroma cacao GN=TCM_001205 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.0e-56
Identity = 110/162 (67.90%), Postives = 137/162 (84.57%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           M ALSE+FSHL+TMT VF TLLLLE V LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALSEYFSHLYTMTIVFFTLLLLEVVILIRSVTGGVCDSEKRLITTTQYLKFIEEKNPT 60

Query: 61  IRFSKKVTSSSD-DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 120
           +R+S + +S  + ++CAVCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR K
Sbjct: 61  VRYSSRSSSRLESNECAVCLSELEEGEEVRKLKCKHTFHKDCLDRWLQQYWATCPLCRTK 120

Query: 121 VLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYRKY 162
           VLPD+IVA Y RL++RVEYDGSDEE+IFLLSALHGNS++R +
Sbjct: 121 VLPDEIVANYHRLQNRVEYDGSDEEMIFLLSALHGNSLHRLF 162

BLAST of CmoCh03G008250 vs. TrEMBL
Match: M5WD85_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012579mg PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.1e-54
Identity = 102/161 (63.35%), Postives = 136/161 (84.47%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALS+FFSHL+T+T +F TLL+LE V L+R+I GL PN+ KRVITT Q+LKLIE+KNPT
Sbjct: 1   MGALSQFFSHLYTITLIFFTLLILEIVILVRSIAGLNPNSSKRVITTTQYLKLIEQKNPT 60

Query: 61  IRFSKKVTSSSDD--QCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 120
             +++K+ S   +  +C+VCLS+FEEG+ +R+L+C HTFH++CLD WL+ ++ATCPLCR 
Sbjct: 61  TCYTQKLRSVRPEATECSVCLSDFEEGDKIRQLKCKHTFHQNCLDRWLQQYWATCPLCRT 120

Query: 121 KVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           KVL DD+VA Y RLR++V+YDGSDEELIFLLS+L GNS++R
Sbjct: 121 KVLSDDVVASYHRLRNQVQYDGSDEELIFLLSSLQGNSLHR 161

BLAST of CmoCh03G008250 vs. TrEMBL
Match: A0A0D2QDA2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G045800 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 3.6e-54
Identity = 106/164 (64.63%), Postives = 133/164 (81.10%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           M AL+EFFSHL+TMT VF TLLLLE   LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALTEFFSHLYTMTVVFFTLLLLEVAILIRSLTGGLSDSEKRLITTTQYLKFIEEKNPT 60

Query: 61  IRFSKKVTSSSD---DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCR 120
           I +S + +S  D   ++C VCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR
Sbjct: 61  IIYSTRSSSRVDLSSNECTVCLSELEEGEKVRKLKCKHTFHKDCLDRWLQQYWATCPLCR 120

Query: 121 CKVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYRKY 162
            KVLPD++VA Y RL+++VEYDGSDEE+IFLLSALH NS++R +
Sbjct: 121 TKVLPDEVVANYHRLQNQVEYDGSDEEMIFLLSALHDNSLHRLF 164

BLAST of CmoCh03G008250 vs. TrEMBL
Match: B9H581_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08300g PE=4 SV=2)

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-52
Identity = 102/160 (63.75%), Postives = 128/160 (80.00%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           M +LSEFFSHL+TM  VF++LLLLE V LIR++ G    +DK +I+T Q+L+ IEEKNPT
Sbjct: 1   MASLSEFFSHLYTMAIVFLSLLLLEIVILIRSVIGSTLKSDKPIISTTQYLRHIEEKNPT 60

Query: 61  IRFSKKVTSSSDD-QCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 120
           I +SK++    D  +CAVCLSEF EGE VRKL+C HTFHKDCLD WL+   ATCPLCR K
Sbjct: 61  ISYSKQLMRQQDSIECAVCLSEFSEGESVRKLKCKHTFHKDCLDEWLQQCLATCPLCRAK 120

Query: 121 VLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           VLPD+I+A Y R++ ++EYDGSDEE+IF+LSALHGNS+ R
Sbjct: 121 VLPDEILAKYDRMQSQIEYDGSDEEMIFMLSALHGNSLQR 160

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT1G15100.1 (AT1G15100.1 RING-H2 finger A2A)

HSP 1 Score: 77.4 bits (189), Expect = 1.1e-14
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 1

Query: 75  CAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRR 132
           C VCLS+ +EGE VRKL+C H FHK CL+ WL  F  TCPLCR  ++ DD V+  +R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT2G04240.1 (AT2G04240.1 RING/U-box superfamily protein)

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-14
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 1

Query: 41  DKRVITTAQFLKLIEEKNPTIRFSK--KVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFH 100
           D RV     +L+    + PT+RF    +    +D++C+VCLS+F+    + KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 101 KDCLDNWLKLFFATCPLCRCK--VLPDD 125
           K CL+ W+  +  TCPLCR    V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT2G01150.1 (AT2G01150.1 RING-H2 finger protein 2B)

HSP 1 Score: 75.1 bits (183), Expect = 5.5e-14
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = 1

Query: 21  LLLLECVTLIRTIFGLR--PNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVC 80
           +LL    T  R +  L   P++   V+ T+    L ++ N    FS + + ++   C VC
Sbjct: 18  MLLALLATFFRHVRSLLLFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVC 77

Query: 81  LSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLP 123
           LS+ + GE VRKL C H FHK CL+ WL+     CPLCR  +LP
Sbjct: 78  LSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 72.4 bits (176), Expect = 3.6e-13
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 1

Query: 59  PTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 118
           PT++ +K +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 119 KVLPDD 125
           ++  DD
Sbjct: 260 ELPTDD 264

BLAST of CmoCh03G008250 vs. TAIR10
Match: AT3G61460.1 (AT3G61460.1 brassinosteroid-responsive RING-H2)

HSP 1 Score: 69.3 bits (168), Expect = 3.0e-12
Identity = 34/76 (44.74%), Postives = 45/76 (59.21%), Query Frame = 1

Query: 53  LIEEKNPTIRFSKKVTSSSD--DQCAVCLSEFEEGEMVRKLQ-CNHTFHKDCLDNWLKLF 112
           LI E  P I+F +   S  D  + CAVCL EFE  + +R L+ C H FH+ CLD W+   
Sbjct: 70  LIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRWMDHD 129

Query: 113 FATCPLCRCKVLPDDI 126
             TCPLCR   +PD++
Sbjct: 130 QKTCPLCRTPFVPDEM 145

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|700193577|gb|KGN48781.1| (hypothetical protein Csa_6G501220 [Cucumis sativus])

HSP 1 Score: 292.4 bits (747), Expect = 6.3e-76
Identity = 145/159 (91.19%), Postives = 147/159 (92.45%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPN+DKRVITTAQF KLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHGNSIYR
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYR 159

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|659080170|ref|XP_008440648.1| (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo])

HSP 1 Score: 282.7 bits (722), Expect = 5.0e-73
Identity = 141/153 (92.16%), Postives = 142/153 (92.81%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPNADKRVITTAQFLKLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 154
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALH
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH 153

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|590707663|ref|XP_007048067.1| (RING-H2 zinc finger protein RHA2a [Theobroma cacao])

HSP 1 Score: 228.0 bits (580), Expect = 1.5e-56
Identity = 110/162 (67.90%), Postives = 137/162 (84.57%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           M ALSE+FSHL+TMT VF TLLLLE V LIR++ G   +++KR+ITT Q+LK IEEKNPT
Sbjct: 1   MAALSEYFSHLYTMTIVFFTLLLLEVVILIRSVTGGVCDSEKRLITTTQYLKFIEEKNPT 60

Query: 61  IRFSKKVTSSSD-DQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCK 120
           +R+S + +S  + ++CAVCLSE EEGE VRKL+C HTFHKDCLD WL+ ++ATCPLCR K
Sbjct: 61  VRYSSRSSSRLESNECAVCLSELEEGEEVRKLKCKHTFHKDCLDRWLQQYWATCPLCRTK 120

Query: 121 VLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYRKY 162
           VLPD+IVA Y RL++RVEYDGSDEE+IFLLSALHGNS++R +
Sbjct: 121 VLPDEIVANYHRLQNRVEYDGSDEEMIFLLSALHGNSLHRLF 162

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|470132684|ref|XP_004302206.1| (PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Fragaria vesca subsp. vesca])

HSP 1 Score: 222.6 bits (566), Expect = 6.1e-55
Identity = 103/161 (63.98%), Postives = 135/161 (83.85%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALS+FFSHL+T++ +F TL  LE V L+R+I GL+PN++KRVITTAQ+LKLI++KNPT
Sbjct: 1   MGALSQFFSHLYTISIIFFTLFALEVVILVRSITGLKPNSEKRVITTAQYLKLIDQKNPT 60

Query: 61  IRFS--KKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 120
           + +S  KK    + ++C+VCLSEFEEGE +R+L+C H FHKDC+D WL+ ++ATCPLCR 
Sbjct: 61  VSYSSKKKNLLRAHEECSVCLSEFEEGEKIRQLKCKHRFHKDCVDKWLQQYWATCPLCRT 120

Query: 121 KVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           KV+PDD+VA Y RLR++VEYDGSDEELIFLLS+L GN+  R
Sbjct: 121 KVVPDDVVASYHRLRNQVEYDGSDEELIFLLSSLQGNNFQR 161

BLAST of CmoCh03G008250 vs. NCBI nr
Match: gi|645303061|ref|XP_008245902.1| (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Prunus mume])

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-54
Identity = 103/161 (63.98%), Postives = 136/161 (84.47%), Query Frame = 1

Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALS+FFSHL+T+T +F TLL+LE V L+R+I GL PN+ KRVITT Q+LKLIE+KNPT
Sbjct: 1   MGALSQFFSHLYTITLIFFTLLILEIVILVRSIAGLNPNSSKRVITTTQYLKLIEQKNPT 60

Query: 61  IRFSKKVTSSSDD--QCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 120
             +++K+ S   +  +C+VCLSEFEEG+ +R+L+C HTFH++CLD WL+ ++ATCPLCR 
Sbjct: 61  TCYTQKLRSVRPEATECSVCLSEFEEGDKIRQLKCKHTFHQNCLDRWLQQYWATCPLCRT 120

Query: 121 KVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           KVL DD+VA Y RLR++V+YDGSDEELIFLLS+L GNS++R
Sbjct: 121 KVLSDDVVASYHRLRNQVQYDGSDEELIFLLSSLQGNSLHR 161

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RHA2A_ARATH2.0e-1356.14E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana GN=RHA2A PE=1 SV=1[more]
XERIC_ARATH3.4e-1338.64Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana GN=XERICO PE... [more]
RHA2B_ARATH9.8e-1337.50E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana GN=RHA2B PE=1 SV=2[more]
RNG1L_ARATH6.4e-1245.45E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1... [more]
RN128_XENLA5.4e-1146.77E3 ubiquitin-protein ligase RNF128 OS=Xenopus laevis GN=rnf128 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KM08_CUCSA4.4e-7691.19Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501220 PE=4 SV=1[more]
A0A061DI69_THECC1.0e-5667.90RING-H2 zinc finger protein RHA2a OS=Theobroma cacao GN=TCM_001205 PE=4 SV=1[more]
M5WD85_PRUPE2.1e-5463.35Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012579mg PE=4 SV=1[more]
A0A0D2QDA2_GOSRA3.6e-5464.63Uncharacterized protein OS=Gossypium raimondii GN=B456_003G045800 PE=4 SV=1[more]
B9H581_POPTR1.5e-5263.75Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08300g PE=4 SV=2[more]
Match NameE-valueIdentityDescription
AT1G15100.11.1e-1456.14 RING-H2 finger A2A[more]
AT2G04240.11.9e-1438.64 RING/U-box superfamily protein[more]
AT2G01150.15.5e-1437.50 RING-H2 finger protein 2B[more]
AT3G19950.13.6e-1345.45 RING/U-box superfamily protein[more]
AT3G61460.13.0e-1244.74 brassinosteroid-responsive RING-H2[more]
Match NameE-valueIdentityDescription
gi|700193577|gb|KGN48781.1|6.3e-7691.19hypothetical protein Csa_6G501220 [Cucumis sativus][more]
gi|659080170|ref|XP_008440648.1|5.0e-7392.16PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo][more]
gi|590707663|ref|XP_007048067.1|1.5e-5667.90RING-H2 zinc finger protein RHA2a [Theobroma cacao][more]
gi|470132684|ref|XP_004302206.1|6.1e-5563.98PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Fragaria vesca subsp. ve... [more]
gi|645303061|ref|XP_008245902.1|1.4e-5463.98PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G008250.1CmoCh03G008250.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 73..117
score: 1.8
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 75..116
score: 2.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 75..117
score: 12
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 70..120
score: 2.1
NoneNo IPR availablePANTHERPTHR14155RING FINGER DOMAIN-CONTAININGcoord: 59..142
score: 6.7
NoneNo IPR availablePANTHERPTHR14155:SF175E3 UBIQUITIN-PROTEIN LIGASE RHA2B-RELATEDcoord: 59..142
score: 6.7
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 64..123
score: 6.48