CSPI06G30530 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G30530
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	E3 ubiquitin-protein ligase RHA2A
Location	Chr6 : 26241608 .. 26242605 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAAAGGAAAATGAAAGAATTGAAAGGAAAAGAGAGAATATAGTGTAGGATGAAATTTTCACAAAGCTTATAAAATTAAAAAGAATTGAATTATTGGGTATCTATCTCTGCCTCCTCCTTCCCCTTCCCCTTCCCCCATCTCTATCCGGACGAAGCTATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCTTCACCCTCTTACTTCTCGAATTCGTTATTCTCATTCGTACCATCTTCGGCTTAAGACCTAACTCCGACAAGCGCGTAATCACGACCGCTCAGTTTTTCAAACTCATCGAAGACAAGAATCCCACCATCCGTTTCTCAAATAAGGTCACCCCAAGTATCGCCGATCAGTGTGCCGTTTGCCTCTCTGAATTCGAGGAAGGAGAGAAGGTACGGAAATTGCAATGCAACCACACTTTTCACAAGGATTGTCTCGATAATTGGTTGAAACTCTGTTTCGCTACCTGTCCGCTTTGCCGGAGTAAGGTTTTGCCGGACGATATCGTCGCCGGCTACCATAGGCTTCGAGACCGTGTAGAGTACGACGGCAGTGACGAGGAGCTCATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGATTTTTCTAGGGCGAAACACCAATTTTATTTTGTTTTTAATCACAGAATAGAATTAAACAACATAGAATGTTGATTTTCTGTAGATTAACTAGGGAAATATTGTTTTTCTCCCCTTCATTGACGAGCAATAACGTTAAGGGAACAGCTCGTTTCTGGTTCGATCGATCTCTCTAGCTAGATCGAGATAAATCTAAATCGTGTATGTTTCTCTTTACTTGCAATTACGCAACCCAACTGTTAGATGTGTTGTTTGCATGATTGCTTCCTACTCTTCTTGGTCATTCGTCAACGAATCATTAATTTTGGAGACACCCAATTTACATCTTGACGGAGACCCTTTTTTTTTTTAACCCAATAACGCAC

mRNA sequence

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCTTCACCCTCTTACTTCTCGAATTCGTTATTCTCATTCGTACCATCTTCGGCTTAAGACCTAACTCCGACAAGCGCGTAATCACGACCGCTCAGTTTTTCAAACTCATCGAAGACAAGAATCCCACCATCCGTTTCTCAAATAAGGTCACCCCAAGTATCGCCGATCAGTGTGCCGTTTGCCTCTCTGAATTCGAGGAAGGAGAGAAGGTACGGAAATTGCAATGCAACCACACTTTTCACAAGGATTGTCTCGATAATTGGTTGAAACTCTGTTTCGCTACCTGTCCGCTTTGCCGGAGTAAGGTTTTGCCGGACGATATCGTCGCCGGCTACCATAGGCTTCGAGACCGTGTAGAGTACGACGGCAGTGACGAGGAGCTCATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGATTTTTCTAG

Coding sequence (CDS)

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCTTCACCCTCTTACTTCTCGAATTCGTTATTCTCATTCGTACCATCTTCGGCTTAAGACCTAACTCCGACAAGCGCGTAATCACGACCGCTCAGTTTTTCAAACTCATCGAAGACAAGAATCCCACCATCCGTTTCTCAAATAAGGTCACCCCAAGTATCGCCGATCAGTGTGCCGTTTGCCTCTCTGAATTCGAGGAAGGAGAGAAGGTACGGAAATTGCAATGCAACCACACTTTTCACAAGGATTGTCTCGATAATTGGTTGAAACTCTGTTTCGCTACCTGTCCGCTTTGCCGGAGTAAGGTTTTGCCGGACGATATCGTCGCCGGCTACCATAGGCTTCGAGACCGTGTAGAGTACGACGGCAGTGACGAGGAGCTCATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGATTTTTCTAG

Homology

[Close]

BLAST of CSPI06G30530 vs. Swiss-Prot
Match: RHA2B_ARATH (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana GN=RHA2B PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 8.4e-15
Identity = 42/101 (41.58%), Postives = 58/101 (57.43%), Query Frame = 1


Query: 22  LLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPTIRFSNKVTPSIADQCAVCLSE 81
           LL  F   +R++  L P+S   V+ T+    L +  N    FS + + + A  C VCLS+
Sbjct: 22  LLATFFRHVRSLL-LFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVCLSK 81

Query: 82  FEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLP 123
            + GE+VRKL C H FHK CL+ WL+     CPLCRS +LP
Sbjct: 82  LKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of CSPI06G30530 vs. Swiss-Prot
Match: RHA2A_ARATH (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana GN=RHA2A PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.1e-13
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 1


Query: 75  CAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLPDDIVAGYHR 132
           C VCLS+ +EGE+VRKL+C H FHK CL+ WL     TCPLCRS ++ DD V+   R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

[Close]

BLAST of CSPI06G30530 vs. Swiss-Prot
Match: XERIC_ARATH (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana GN=XERICO PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.3e-12
Identity = 32/88 (36.36%), Postives = 52/88 (59.09%), Query Frame = 1


Query: 41  DKRVITTAQFFKLIEDKNPTIRFSN--KVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFH 100
           D RV     + +   ++ PT+RF +  +      ++C+VCLS+F+   ++ KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 101 KDCLDNWLKLCFATCPLCRSK--VLPDD 125
           K CL+ W+     TCPLCR+   V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

[Close]

BLAST of CSPI06G30530 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.1e-12
Identity = 30/69 (43.48%), Postives = 46/69 (66.67%), Query Frame = 1


Query: 56  DKNPTIRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPL 115
           D  PT++ +  +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+
Sbjct: 197 DALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPV 256

Query: 116 CRSKVLPDD 125
           CR ++  DD
Sbjct: 257 CRFELPTDD 264

[Close]

BLAST of CSPI06G30530 vs. Swiss-Prot
Match: RHA1B_ARATH (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana GN=RHA1B PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.3e-11
Identity = 34/87 (39.08%), Postives = 51/87 (58.62%), Query Frame = 1


Query: 40  SDKRVITTAQFFKLIEDKNPTIRFSNKVTPSIADQCAVCLSEFEEGEKVRKL-QCNHTFH 99
           SD   +  +    L  +  P +RFS+ +T    D C VCLS+F   +K+R+L +C H FH
Sbjct: 51  SDPTRLALSTSATLANELIPVVRFSDLLTDP-EDCCTVCLSDFVSDDKIRQLPKCGHVFH 110

Query: 100 KDCLDNWLKLC-FATCPLCRSKVLPDD 125
             CLD W+  C   TCP+CR++ LP++
Sbjct: 111 HRCLDRWIVDCNKITCPICRNRFLPEE 136

[Close]

BLAST of CSPI06G30530 vs. TrEMBL
Match: A0A0A0KM08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501220 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 3.1e-88
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 162
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 161

[Close]

BLAST of CSPI06G30530 vs. TrEMBL
Match: A0A061DI69_THECC (RING-H2 zinc finger protein RHA2a OS=Theobroma cacao GN=TCM_001205 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 1.7e-59
Identity = 112/162 (69.14%), Postives = 141/162 (87.04%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           M ALSE+FSHL+TMT VFFTLLLLE VILIR++ G   +S+KR+ITT Q+ K IE+KNPT
Sbjct: 1   MAALSEYFSHLYTMTIVFFTLLLLEVVILIRSVTGGVCDSEKRLITTTQYLKFIEEKNPT 60

Query: 61  IRFSNKVTPSI-ADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSK 120
           +R+S++ +  + +++CAVCLSE EEGE+VRKL+C HTFHKDCLD WL+  +ATCPLCR+K
Sbjct: 61  VRYSSRSSSRLESNECAVCLSELEEGEEVRKLKCKHTFHKDCLDRWLQQYWATCPLCRTK 120

Query: 121 VLPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 162
           VLPD+IVA YHRL++RVEYDGSDEE+IFLLSALHGNS++R F
Sbjct: 121 VLPDEIVANYHRLQNRVEYDGSDEEMIFLLSALHGNSLHRLF 162

[Close]

BLAST of CSPI06G30530 vs. TrEMBL
Match: M5WD85_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012579mg PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 2.5e-58
Identity = 110/164 (67.07%), Postives = 139/164 (84.76%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           MGALS+FFSHL+T+T +FFTLL+LE VIL+R+I GL PNS KRVITT Q+ KLIE KNPT
Sbjct: 1   MGALSQFFSHLYTITLIFFTLLILEIVILVRSIAGLNPNSSKRVITTTQYLKLIEQKNPT 60

Query: 61  IRFSNK---VTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCR 120
             ++ K   V P  A +C+VCLS+FEEG+K+R+L+C HTFH++CLD WL+  +ATCPLCR
Sbjct: 61  TCYTQKLRSVRPE-ATECSVCLSDFEEGDKIRQLKCKHTFHQNCLDRWLQQYWATCPLCR 120

Query: 121 SKVLPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 162
           +KVL DD+VA YHRLR++V+YDGSDEELIFLLS+L GNS++RFF
Sbjct: 121 TKVLSDDVVASYHRLRNQVQYDGSDEELIFLLSSLQGNSLHRFF 163

[Close]

BLAST of CSPI06G30530 vs. TrEMBL
Match: A0A0D2QDA2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G045800 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 6.2e-57
Identity = 108/164 (65.85%), Postives = 136/164 (82.93%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           M AL+EFFSHL+TMT VFFTLLLLE  ILIR++ G   +S+KR+ITT Q+ K IE+KNPT
Sbjct: 1   MAALTEFFSHLYTMTVVFFTLLLLEVAILIRSLTGGLSDSEKRLITTTQYLKFIEEKNPT 60

Query: 61  IRFSNKVTPSI---ADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCR 120
           I +S + +  +   +++C VCLSE EEGEKVRKL+C HTFHKDCLD WL+  +ATCPLCR
Sbjct: 61  IIYSTRSSSRVDLSSNECTVCLSELEEGEKVRKLKCKHTFHKDCLDRWLQQYWATCPLCR 120

Query: 121 SKVLPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 162
           +KVLPD++VA YHRL+++VEYDGSDEE+IFLLSALH NS++R F
Sbjct: 121 TKVLPDEVVANYHRLQNQVEYDGSDEEMIFLLSALHDNSLHRLF 164

[Close]

BLAST of CSPI06G30530 vs. TrEMBL
Match: B9H581_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08300g PE=4 SV=2)

HSP 1 Score: 217.2 bits (552), Expect = 1.4e-53
Identity = 102/162 (62.96%), Postives = 129/162 (79.63%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           M +LSEFFSHL+TM  VF +LLLLE VILIR++ G    SDK +I+T Q+ + IE+KNPT
Sbjct: 1   MASLSEFFSHLYTMAIVFLSLLLLEIVILIRSVIGSTLKSDKPIISTTQYLRHIEEKNPT 60

Query: 61  IRFSNKVT-PSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSK 120
           I +S ++     + +CAVCLSEF EGE VRKL+C HTFHKDCLD WL+ C ATCPLCR+K
Sbjct: 61  ISYSKQLMRQQDSIECAVCLSEFSEGESVRKLKCKHTFHKDCLDEWLQQCLATCPLCRAK 120

Query: 121 VLPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 162
           VLPD+I+A Y R++ ++EYDGSDEE+IF+LSALHGNS+ R F
Sbjct: 121 VLPDEILAKYDRMQSQIEYDGSDEEMIFMLSALHGNSLQRIF 162

[Close]

BLAST of CSPI06G30530 vs. TAIR10
Match: AT2G01150.1 (AT2G01150.1 RING-H2 finger protein 2B)

HSP 1 Score: 81.6 bits (200), Expect = 4.7e-16
Identity = 42/101 (41.58%), Postives = 58/101 (57.43%), Query Frame = 1


Query: 22  LLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPTIRFSNKVTPSIADQCAVCLSE 81
           LL  F   +R++  L P+S   V+ T+    L +  N    FS + + + A  C VCLS+
Sbjct: 22  LLATFFRHVRSLL-LFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVCLSK 81

Query: 82  FEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLP 123
            + GE+VRKL C H FHK CL+ WL+     CPLCRS +LP
Sbjct: 82  LKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of CSPI06G30530 vs. TAIR10
Match: AT1G15100.1 (AT1G15100.1 RING-H2 finger A2A)

HSP 1 Score: 77.0 bits (188), Expect = 1.2e-14
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 1


Query: 75  CAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLPDDIVAGYHR 132
           C VCLS+ +EGE+VRKL+C H FHK CL+ WL     TCPLCRS ++ DD V+   R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

[Close]

BLAST of CSPI06G30530 vs. TAIR10
Match: AT2G04240.1 (AT2G04240.1 RING/U-box superfamily protein)

HSP 1 Score: 73.6 bits (179), Expect = 1.3e-13
Identity = 32/88 (36.36%), Postives = 52/88 (59.09%), Query Frame = 1


Query: 41  DKRVITTAQFFKLIEDKNPTIRFSN--KVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFH 100
           D RV     + +   ++ PT+RF +  +      ++C+VCLS+F+   ++ KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 101 KDCLDNWLKLCFATCPLCRSK--VLPDD 125
           K CL+ W+     TCPLCR+   V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

[Close]

BLAST of CSPI06G30530 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 72.4 bits (176), Expect = 2.9e-13
Identity = 30/69 (43.48%), Postives = 46/69 (66.67%), Query Frame = 1


Query: 56  DKNPTIRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPL 115
           D  PT++ +  +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+
Sbjct: 197 DALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPV 256

Query: 116 CRSKVLPDD 125
           CR ++  DD
Sbjct: 257 CRFELPTDD 264

[Close]

BLAST of CSPI06G30530 vs. TAIR10
Match: AT4G11360.1 (AT4G11360.1 RING-H2 finger A1B)

HSP 1 Score: 69.7 bits (169), Expect = 1.9e-12
Identity = 34/87 (39.08%), Postives = 51/87 (58.62%), Query Frame = 1


Query: 40  SDKRVITTAQFFKLIEDKNPTIRFSNKVTPSIADQCAVCLSEFEEGEKVRKL-QCNHTFH 99
           SD   +  +    L  +  P +RFS+ +T    D C VCLS+F   +K+R+L +C H FH
Sbjct: 51  SDPTRLALSTSATLANELIPVVRFSDLLTDP-EDCCTVCLSDFVSDDKIRQLPKCGHVFH 110

Query: 100 KDCLDNWLKLC-FATCPLCRSKVLPDD 125
             CLD W+  C   TCP+CR++ LP++
Sbjct: 111 HRCLDRWIVDCNKITCPICRNRFLPEE 136

[Close]

BLAST of CSPI06G30530 vs. NCBI nr
Match: gi|700193577|gb|KGN48781.1| (hypothetical protein Csa_6G501220 [Cucumis sativus])

HSP 1 Score: 332.4 bits (851), Expect = 4.4e-88
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 162
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 161

[Close]

BLAST of CSPI06G30530 vs. NCBI nr
Match: gi|659080170|ref|XP_008440648.1| (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo])

HSP 1 Score: 311.6 bits (797), Expect = 8.0e-82
Identity = 151/153 (98.69%), Postives = 152/153 (99.35%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPN+DKRVITTAQF KLIEDKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH 154
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH 153

[Close]

BLAST of CSPI06G30530 vs. NCBI nr
Match: gi|590707663|ref|XP_007048067.1| (RING-H2 zinc finger protein RHA2a [Theobroma cacao])

HSP 1 Score: 236.9 bits (603), Expect = 2.5e-59
Identity = 112/162 (69.14%), Postives = 141/162 (87.04%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           M ALSE+FSHL+TMT VFFTLLLLE VILIR++ G   +S+KR+ITT Q+ K IE+KNPT
Sbjct: 1   MAALSEYFSHLYTMTIVFFTLLLLEVVILIRSVTGGVCDSEKRLITTTQYLKFIEEKNPT 60

Query: 61  IRFSNKVTPSI-ADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSK 120
           +R+S++ +  + +++CAVCLSE EEGE+VRKL+C HTFHKDCLD WL+  +ATCPLCR+K
Sbjct: 61  VRYSSRSSSRLESNECAVCLSELEEGEEVRKLKCKHTFHKDCLDRWLQQYWATCPLCRTK 120

Query: 121 VLPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 162
           VLPD+IVA YHRL++RVEYDGSDEE+IFLLSALHGNS++R F
Sbjct: 121 VLPDEIVANYHRLQNRVEYDGSDEEMIFLLSALHGNSLHRLF 162

[Close]

BLAST of CSPI06G30530 vs. NCBI nr
Match: gi|470132684|ref|XP_004302206.1| (PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Fragaria vesca subsp. vesca])

HSP 1 Score: 235.0 bits (598), Expect = 9.5e-59
Identity = 108/163 (66.26%), Postives = 139/163 (85.28%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           MGALS+FFSHL+T++ +FFTL  LE VIL+R+I GL+PNS+KRVITTAQ+ KLI+ KNPT
Sbjct: 1   MGALSQFFSHLYTISIIFFTLFALEVVILVRSITGLKPNSEKRVITTAQYLKLIDQKNPT 60

Query: 61  IRFSNKVTPSIA--DQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRS 120
           + +S+K    +   ++C+VCLSEFEEGEK+R+L+C H FHKDC+D WL+  +ATCPLCR+
Sbjct: 61  VSYSSKKKNLLRAHEECSVCLSEFEEGEKIRQLKCKHRFHKDCVDKWLQQYWATCPLCRT 120

Query: 121 KVLPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 162
           KV+PDD+VA YHRLR++VEYDGSDEELIFLLS+L GN+  RFF
Sbjct: 121 KVVPDDVVASYHRLRNQVEYDGSDEELIFLLSSLQGNNFQRFF 163

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BLAST of CSPI06G30530 vs. NCBI nr
Match: gi|645303061|ref|XP_008245902.1| (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Prunus mume])

HSP 1 Score: 234.2 bits (596), Expect = 1.6e-58
Identity = 111/164 (67.68%), Postives = 139/164 (84.76%), Query Frame = 1


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60
           MGALS+FFSHL+T+T +FFTLL+LE VIL+R+I GL PNS KRVITT Q+ KLIE KNPT
Sbjct: 1   MGALSQFFSHLYTITLIFFTLLILEIVILVRSIAGLNPNSSKRVITTTQYLKLIEQKNPT 60

Query: 61  IRFSNK---VTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCR 120
             ++ K   V P  A +C+VCLSEFEEG+K+R+L+C HTFH++CLD WL+  +ATCPLCR
Sbjct: 61  TCYTQKLRSVRPE-ATECSVCLSEFEEGDKIRQLKCKHTFHQNCLDRWLQQYWATCPLCR 120

Query: 121 SKVLPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYRFF 162
           +KVL DD+VA YHRLR++V+YDGSDEELIFLLS+L GNS++RFF
Sbjct: 121 TKVLSDDVVASYHRLRNQVQYDGSDEELIFLLSSLQGNSLHRFF 163

The following BLAST results are available for this feature:

BLAST of CSPI06G30530 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RHA2B_ARATH	8.4e-15	41.58	E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana GN=RHA2B PE=1 SV=2	[more]
RHA2A_ARATH	2.1e-13	56.14	E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana GN=RHA2A PE=1 SV=1	[more]
XERIC_ARATH	2.3e-12	36.36	Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana GN=XERICO PE...	[more]
RNG1L_ARATH	5.1e-12	43.48	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1...	[more]
RHA1B_ARATH	3.3e-11	39.08	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana GN=RHA1B PE=2 SV=1	[more]

BLAST of CSPI06G30530 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KM08_CUCSA	3.1e-88	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501220 PE=4 SV=1	[more]
A0A061DI69_THECC	1.7e-59	69.14	RING-H2 zinc finger protein RHA2a OS=Theobroma cacao GN=TCM_001205 PE=4 SV=1	[more]
M5WD85_PRUPE	2.5e-58	67.07	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012579mg PE=4 SV=1	[more]
A0A0D2QDA2_GOSRA	6.2e-57	65.85	Uncharacterized protein OS=Gossypium raimondii GN=B456_003G045800 PE=4 SV=1	[more]
B9H581_POPTR	1.4e-53	62.96	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08300g PE=4 SV=2	[more]

BLAST of CSPI06G30530 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G01150.1	4.7e-16	41.58	RING-H2 finger protein 2B	[more]
AT1G15100.1	1.2e-14	56.14	RING-H2 finger A2A	[more]
AT2G04240.1	1.3e-13	36.36	RING/U-box superfamily protein	[more]
AT3G19950.1	2.9e-13	43.48	RING/U-box superfamily protein	[more]
AT4G11360.1	1.9e-12	39.08	RING-H2 finger A1B	[more]

BLAST of CSPI06G30530 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700193577\|gb\|KGN48781.1\|	4.4e-88	100.00	hypothetical protein Csa_6G501220 [Cucumis sativus]	[more]
gi\|659080170\|ref\|XP_008440648.1\|	8.0e-82	98.69	PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo]	[more]
gi\|590707663\|ref\|XP_007048067.1\|	2.5e-59	69.14	RING-H2 zinc finger protein RHA2a [Theobroma cacao]	[more]
gi\|470132684\|ref\|XP_004302206.1\|	9.5e-59	66.26	PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Fragaria vesca subsp. ve...	[more]
gi\|645303061\|ref\|XP_008245902.1\|	1.6e-58	67.68	PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G30530.1	CSPI06G30530.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 73..117 score: 1.3
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 75..116 score: 1.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 75..117 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 73..120 score: 1.1
None	No IPR available	PANTHER	PTHR14155	RING FINGER DOMAIN-CONTAINING	coord: 59..142 score: 1.2
None	No IPR available	PANTHER	PTHR14155:SF175	E3 UBIQUITIN-PROTEIN LIGASE RHA2B-RELATED	coord: 59..142 score: 1.2
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 74..123 score: 1.01

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI06G30530	Cla014172	Watermelon (97103) v1	cpiwmB519
CSPI06G30530	Csa6G501220	Cucumber (Chinese Long) v2	cpicuB313
CSPI06G30530	MELO3C007785	Melon (DHL92) v3.5.1	cpimeB508
CSPI06G30530	ClCG01G019070	Watermelon (Charleston Gray)	cpiwcgB453
CSPI06G30530	Lsi01G007320	Bottle gourd (USVL1VR-Ls)	cpilsiB439
CSPI06G30530	MELO3C007785.2	Melon (DHL92) v3.6.1	cpimedB493
CSPI06G30530	CsaV3_6G045750	Cucumber (Chinese Long) v3	cpicucB363
CSPI06G30530	Cla97C01G018530	Watermelon (97103) v2	cpiwmbB433
CSPI06G30530	Bhi03G000946	Wax gourd	cpiwgoB627
CSPI06G30530	Cucsa.104680	Cucumber (Gy14) v1	cgycpiB122
CSPI06G30530	CmaCh07G005200	Cucurbita maxima (Rimu)	cmacpiB893
CSPI06G30530	CmaCh03G007910	Cucurbita maxima (Rimu)	cmacpiB689
CSPI06G30530	CmoCh03G008250	Cucurbita moschata (Rifu)	cmocpiB681
CSPI06G30530	CmoCh07G005150	Cucurbita moschata (Rifu)	cmocpiB873
CSPI06G30530	Cp4.1LG19g09770	Cucurbita pepo (Zucchini)	cpecpiB503
CSPI06G30530	Cp4.1LG10g01110	Cucurbita pepo (Zucchini)	cpecpiB080
CSPI06G30530	CsGy6G030020	Cucumber (Gy14) v2	cgybcpiB287
CSPI06G30530	Carg16020	Silver-seed gourd	carcpiB0794
CSPI06G30530	Carg00676	Silver-seed gourd	carcpiB0446

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI06G30530	Cucurbita maxima (Rimu)	cmacpiB785
CSPI06G30530	Cucurbita moschata (Rifu)	cmocpiB775
CSPI06G30530	Cucurbita pepo (Zucchini)	cpecpiB468
CSPI06G30530	Silver-seed gourd	carcpiB0982