MELO3C002305 (gene) Melon (DHL92) v3.5.1

Overview
NameMELO3C002305
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionHigh-affinity nitrate transporter 3.2-like protein
Locationchr12 : 23856500 .. 23857631 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCAATAACACCACCATCATCGTCACGTCTGAGATTCACTTCACTTTTTGTCTACAAACTTTAAAAGGGTTTAATCAAATCAATATGGCTTCAAATGGGTTTCTCTTGGCTGCTCTGTTCCTGTCTTGTTTCTTGGGATTTTCTCTTGGAAATATCCATCTCTCTTCTCTTCCAAGAACTTTGGAGGTCACTGCTTCACCGAAACCTGGACAAGGTAAGCTAAAACACAAAACCCTGATTCTTTCATGGAGATCTGAAATCAAATATTCAATCTTTACCAATTTAAACAACTGGGCTGTGATTTTTTTTTTTTCTGCAGTTCTTAAAGCGGGTGTTGATAAAATCAGGGTGACATGGGTTTTAAATGGGACCGTCAAAGCTGGGTCGGATTCGAGTTACAAGAACATCAAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAAGTGGACAGAGCGTGGAGGAAAACAGAGGACGATCTTAAAAAGGATAAGACTTGTCAATTCGACATCGTGGAAAAGCCGTACAATCCAGCGAACAAGACGGCTCAAAGCTTTGAGTGGACGGTGGAGCGTGACGTTCCGACGGGGACTTTCTTCGTTAGAGCCTACGTTTTTAATTCTGCCGGCCAAGAAGTGGCGTACGGCCAAACCACAGATGATAAGAAAGGAACGAACTTGTTTCAAATTGAATCGATTACTGGTCGTCATGTTTCATTAGATATTGCCTCCGTTTGCTTCAGTGCTTTCTCTGTGGTATCGTTGTTCGGATTCTTCTTCATCGACAAGAGGAAGGCCAAGAAGGCGGCGGCGGCGGCGGAGGCGTCAGGGCATTAAGTTAAGAAAGGTGGGAACTTGCTCTGTTTTGAGTTTCCTTCTCAGCTTGTGACAATGGTGATCTGCTTGATCAAGAATAGACTTTATGAGTTTCATTATTGTTATTAACTTTTTGAAGTCTTTTTGCATTTACTGTGATTTTCTAGGGGAAAAAAGAATCGGATGTGTTGAGAAAAAGAAGGGTTTGGTATGATATGATATATGGTGGAGAATAAAAGTGTACTAATGTGTTTTAATGTTGTTGTGGAAATTTTAATGACAATATTTTAATATTTATTGGCATAAATTCATGGCCAAAAGG

mRNA sequence

ATCAATAACACCACCATCATCGTCACGTCTGAGATTCACTTCACTTTTTGTCTACAAACTTTAAAAGGGTTTAATCAAATCAATATGGCTTCAAATGGGTTTCTCTTGGCTGCTCTGTTCCTGTCTTGTTTCTTGGGATTTTCTCTTGGAAATATCCATCTCTCTTCTCTTCCAAGAACTTTGGAGGTCACTGCTTCACCGAAACCTGGACAAGTTCTTAAAGCGGGTGTTGATAAAATCAGGGTGACATGGGTTTTAAATGGGACCGTCAAAGCTGGGTCGGATTCGAGTTACAAGAACATCAAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAAGTGGACAGAGCGTGGAGGAAAACAGAGGACGATCTTAAAAAGGATAAGACTTGTCAATTCGACATCGTGGAAAAGCCGTACAATCCAGCGAACAAGACGGCTCAAAGCTTTGAGTGGACGGTGGAGCGTGACGTTCCGACGGGGACTTTCTTCGTTAGAGCCTACGTTTTTAATTCTGCCGGCCAAGAAGTGGCGTACGGCCAAACCACAGATGATAAGAAAGGAACGAACTTGTTTCAAATTGAATCGATTACTGGTCGTCATGTTTCATTAGATATTGCCTCCGTTTGCTTCAGTGCTTTCTCTGTGGTATCGTTGTTCGGATTCTTCTTCATCGACAAGAGGAAGGCCAAGAAGGCGGCGGCGGCGGCGGAGGCGTCAGGGCATTAAGTTAAGAAAGGTGGGAACTTGCTCTGTTTTGAGTTTCCTTCTCAGCTTGTGACAATGGTGATCTGCTTGATCAAGAATAGACTTTATGAGTTTCATTATTGTTATTAACTTTTTGAAGTCTTTTTGCATTTACTGTGATTTTCTAGGGGAAAAAAGAATCGGATGTGTTGAGAAAAAGAAGGGTTTGGTATGATATGATATATGGTGGAGAATAAAAGTGTACTAATGTGTTTTAATGTTGTTGTGGAAATTTTAATGACAATATTTTAATATTTATTGGCATAAATTCATGGCCAAAAGG

Coding sequence (CDS)

ATGGCTTCAAATGGGTTTCTCTTGGCTGCTCTGTTCCTGTCTTGTTTCTTGGGATTTTCTCTTGGAAATATCCATCTCTCTTCTCTTCCAAGAACTTTGGAGGTCACTGCTTCACCGAAACCTGGACAAGTTCTTAAAGCGGGTGTTGATAAAATCAGGGTGACATGGGTTTTAAATGGGACCGTCAAAGCTGGGTCGGATTCGAGTTACAAGAACATCAAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAAGTGGACAGAGCGTGGAGGAAAACAGAGGACGATCTTAAAAAGGATAAGACTTGTCAATTCGACATCGTGGAAAAGCCGTACAATCCAGCGAACAAGACGGCTCAAAGCTTTGAGTGGACGGTGGAGCGTGACGTTCCGACGGGGACTTTCTTCGTTAGAGCCTACGTTTTTAATTCTGCCGGCCAAGAAGTGGCGTACGGCCAAACCACAGATGATAAGAAAGGAACGAACTTGTTTCAAATTGAATCGATTACTGGTCGTCATGTTTCATTAGATATTGCCTCCGTTTGCTTCAGTGCTTTCTCTGTGGTATCGTTGTTCGGATTCTTCTTCATCGACAAGAGGAAGGCCAAGAAGGCGGCGGCGGCGGCGGAGGCGTCAGGGCATTAA

Protein sequence

MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNGTVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAKKAAAAAEASGH*
Homology
[Close]
BLAST of MELO3C002305 vs. Swiss-Prot
Match: NRT31_ARATH (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 5.7e-51
Identity = 101/173 (58.38%), Postives = 125/173 (72.25%), Query Frame = 1 

Query: 33  LEVTASPK---PGQVLKAGVDKIRVTWVLNGTVKAGSDSSYKNIKAKLCYAPVSQVDRAW 92
           L+VT  P    PG VL AG D + +TW L+ ++ +  ++ +K IK KLCYAP SQVDR W
Sbjct: 37  LDVTTKPSREGPGVVLDAGKDTLNITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPW 96

Query: 93  RKTEDDLKKDKTCQFDIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVA 152
           RKT D+L KDKTC   I+ KPY+   KT QS  WT+ERD+PTGT+FVRAY  ++ G EVA
Sbjct: 97  RKTHDELFKDKTCPHKIIAKPYD---KTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVA 156

Query: 153 YGQTTDDKKGTNLFQIESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAK 203
           YGQ+TDD K TNLF +++I+GRH SLDIAS+CFS FSVV+L  FF  +KRKAK
Sbjct: 157 YGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]
BLAST of MELO3C002305 vs. Swiss-Prot
Match: NRT32_ARATH (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.0e-48
Identity = 102/207 (49.28%), Postives = 137/207 (66.18%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIH---LSSLPRTLEVTASP-KPGQVLKAGVDKIRVTW 60
           MA +  L  +L +   +  S G       + L  ++EVTA P K   VL+AG D + +TW
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 61  VL-NGTVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPAN 120
            L + + K  +D+++K I+ KLCYAP+SQVDR WRKT++ L KD++C  +IV K Y+   
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAYD--- 120

Query: 121 KTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSL 180
           KT QS +WT+  D+PTGT+FVRAY  +  G EVAYGQ+TD+ + TNLF + +I+G HV L
Sbjct: 121 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 180

Query: 181 DIASVCFSAFSVVSLFGFFFIDKRKAK 203
           DIAS  FS FSVVSLF FF ++KRKAK
Sbjct: 181 DIASTFFSVFSVVSLFVFFVMEKRKAK 204

[Close]
BLAST of MELO3C002305 vs. Swiss-Prot
Match: NAR21_ORYSJ (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.2e-42
Identity = 85/180 (47.22%), Postives = 116/180 (64.44%), Query Frame = 1 

Query: 24  IHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNGTVKAGSDSSYKNIKAKLCYAPVS 83
           + LS LP+ L V  SPK G+V+ AG + + VTW LN +  AG+D+++K++K KLCYAP S
Sbjct: 34  VFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPAS 93

Query: 84  QVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNS 143
           + DR WRK  DDL KDK CQF +  +PY      A  F++ V RD+PT ++FVRAY  ++
Sbjct: 94  RTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDA 153

Query: 144 AGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAKK 203
           +G EVAYGQ++ D      F +  ITG H SL +A+  FS FS+ +L  FF ++KRK  K
Sbjct: 154 SGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

[Close]
BLAST of MELO3C002305 vs. Swiss-Prot
Match: NAR22_ORYSJ (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 2.6e-40
Identity = 88/181 (48.62%), Postives = 112/181 (61.88%), Query Frame = 1 

Query: 26  LSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNGTVKA---GSDSSYKNIKAKLCYAPV 85
           LS+L + L V ASP+ GQVL AG D I VTW LN T  A   G+D+ YK +K  LCYAP 
Sbjct: 38  LSALAKALVVEASPRAGQVLHAGEDAITVTWSLNATAAAAAAGADAGYKAVKVTLCYAPA 97

Query: 86  SQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFN 145
           SQV R WRK  DDL KDK CQF I ++PY+ A K    FE+TV RDVPT +++VRAY  +
Sbjct: 98  SQVGRGWRKAHDDLSKDKACQFKIAQQPYDGAGK----FEYTVARDVPTASYYVRAYALD 157

Query: 146 SAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAK 204
           ++G  VAYG+T         F +  ITG   S+++A+   SAFSV +L  F  ++ +K  
Sbjct: 158 ASGARVAYGETAPSAS----FAVAGITGVTASIEVAAGVLSAFSVAALAVFLVLENKKKN 210

[Close]
BLAST of MELO3C002305 vs. TrEMBL
Match: C8CHG8_CUCSA (High-affinity nitrate transport system component OS=Cucumis sativus GN=NAR2 PE=2 SV=2)

HSP 1 Score: 387.9 bits (995), Expect = 8.1e-105
Identity = 193/208 (92.79%), Postives = 197/208 (94.71%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MASN FLLAALFLSCFLGFS GNIH SSLPRTLEVTASPKPGQVLKAGVDKI VTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQF IVEK YNPANKT QS
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV+RD+PTGTFFVRAYV NSAG+EVAYGQTTD KKGTNLFQIESITGRH+SLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAA 209
           CFSAFSVVSLFGFFFIDKRKAKKAAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAA 208

[Close]
BLAST of MELO3C002305 vs. TrEMBL
Match: A0A067K4F8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11393 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 9.6e-74
Identity = 144/203 (70.94%), Postives = 160/203 (78.82%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MA  G  LA++ L  F G S G IH SSL +TL VTASP PGQVLKAGVD I VTW LN 
Sbjct: 1   MAPRGLFLASILLFAFFGSSYGVIHFSSLQKTLLVTASPSPGQVLKAGVDNITVTWGLNQ 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           ++ AG+DS+YK IK KLCYAPVSQ DRAWRKTED+L KDKTCQ  IV KPY+ ANK   S
Sbjct: 61  SLPAGTDSAYKTIKIKLCYAPVSQKDRAWRKTEDNLSKDKTCQVKIVTKPYSSANKN-NS 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           F WTVERDVPT T+FVRAY FN+  QEVAYGQTTD  K TN F I++ITGRH SLDIASV
Sbjct: 121 FTWTVERDVPTATYFVRAYAFNANEQEVAYGQTTDVHKTTNFFDIQAITGRHASLDIASV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKK 204
           CFS FSVV+LFGFF+I+KRKAKK
Sbjct: 181 CFSVFSVVALFGFFYIEKRKAKK 202

[Close]
BLAST of MELO3C002305 vs. TrEMBL
Match: A0A0S3R655_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G397600 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 6.2e-73
Identity = 132/207 (63.77%), Postives = 166/207 (80.19%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MA++  ++A+L + C  G   G +H SSL +TL+VTASPK GQVL+AG DKI VTW LN 
Sbjct: 1   MAAHSLVVASLLIFCLAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           T+ +G+DS+YK IK KLCYAP+SQ DRAWRKT D+L +DKTCQ  IV KPY+ +N+T Q 
Sbjct: 61  TLPSGTDSAYKTIKVKLCYAPISQKDRAWRKTVDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEW +ERDVP  T+FVRAYVF+S G+EVAYGQTTD KK +NLF+I SI+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYVFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 208
           CFSAFSVV+LF FF+I+KRK K ++++
Sbjct: 181 CFSAFSVVALFVFFYIEKRKGKASSSS 207

[Close]
BLAST of MELO3C002305 vs. TrEMBL
Match: A0A0L9TQ82_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g230400 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 6.2e-73
Identity = 132/207 (63.77%), Postives = 166/207 (80.19%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MA++  ++A+L + C  G   G +H SSL +TL+VTASPK GQVL+AG DKI VTW LN 
Sbjct: 1   MAAHSLVVASLLIFCLAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           T+ +G+DS+YK IK KLCYAP+SQ DRAWRKT D+L +DKTCQ  IV KPY+ +N+T Q 
Sbjct: 61  TLPSGTDSAYKTIKVKLCYAPISQKDRAWRKTVDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEW +ERDVP  T+FVRAYVF+S G+EVAYGQTTD KK +NLF+I SI+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYVFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 208
           CFSAFSVV+LF FF+I+KRK K ++++
Sbjct: 181 CFSAFSVVALFVFFYIEKRKGKASSSS 207

[Close]
BLAST of MELO3C002305 vs. TrEMBL
Match: B9P5X4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0015s09670g PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 1.8e-72
Identity = 138/204 (67.65%), Postives = 163/204 (79.90%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MA+   LLA+L LSCFL    G +  SSL RTL VTASP  GQVLK GVDKI VTW LN 
Sbjct: 1   MAARALLLASLVLSCFLLPCYGIVLFSSLHRTLVVTASPTSGQVLKGGVDKITVTWGLNQ 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           TV AG+DS+YK IK KLCYAP+SQVDR WRKT D+LKKD+TCQ  IV +PYNPAN TAQS
Sbjct: 61  TVAAGTDSTYKTIKVKLCYAPISQVDRGWRKTVDNLKKDRTCQHKIVARPYNPANSTAQS 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
            EWTVERDVPT T+FVRAY +++  + VAYGQTTD  K TNLFQ+++I+GRHV++D  S+
Sbjct: 121 HEWTVERDVPTATYFVRAYAYDADEKVVAYGQTTDAHKTTNLFQVQAISGRHVTMDTCSI 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKA 205
           CFS FSVVSLFGFF+ +KRKAK++
Sbjct: 181 CFSVFSVVSLFGFFYNEKRKAKRS 204

[Close]
BLAST of MELO3C002305 vs. TAIR10
Match: AT5G50200.3 (AT5G50200.3 nitrate transmembrane transporters)

HSP 1 Score: 202.2 bits (513), Expect = 3.2e-52
Identity = 101/173 (58.38%), Postives = 125/173 (72.25%), Query Frame = 1 

Query: 33  LEVTASPK---PGQVLKAGVDKIRVTWVLNGTVKAGSDSSYKNIKAKLCYAPVSQVDRAW 92
           L+VT  P    PG VL AG D + +TW L+ ++ +  ++ +K IK KLCYAP SQVDR W
Sbjct: 37  LDVTTKPSREGPGVVLDAGKDTLNITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPW 96

Query: 93  RKTEDDLKKDKTCQFDIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVA 152
           RKT D+L KDKTC   I+ KPY+   KT QS  WT+ERD+PTGT+FVRAY  ++ G EVA
Sbjct: 97  RKTHDELFKDKTCPHKIIAKPYD---KTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVA 156

Query: 153 YGQTTDDKKGTNLFQIESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAK 203
           YGQ+TDD K TNLF +++I+GRH SLDIAS+CFS FSVV+L  FF  +KRKAK
Sbjct: 157 YGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]
BLAST of MELO3C002305 vs. TAIR10
Match: AT4G24730.1 (AT4G24730.1 Calcineurin-like metallo-phosphoesterase superfamily protein)

HSP 1 Score: 186.0 bits (471), Expect = 2.4e-47
Identity = 93/173 (53.76%), Postives = 122/173 (70.52%), Query Frame = 1 

Query: 31  RTLEVTASPKPGQVLKAGVDKIRVTWVL-NGTVKAGSDSSYKNIKAKLCYAPVSQVDRAW 90
           R+LE      P  VL+AG D + +TW L + + K  +D+++K I+ KLCYAP+SQVDR W
Sbjct: 271 RSLEAALECPP--VLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPW 330

Query: 91  RKTEDDLKKDKTCQFDIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVA 150
           RKT++ L KD++C  +IV K Y+   KT QS +WT+  D+PTGT+FVRAY  +  G EVA
Sbjct: 331 RKTDNKLFKDRSCPHEIVSKAYD---KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVA 390

Query: 151 YGQTTDDKKGTNLFQIESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAK 203
           YGQ+TD+ + TNLF + +I+G HV LDIAS  FS FSVVSLF FF ++KRKAK
Sbjct: 391 YGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 438

[Close]
BLAST of MELO3C002305 vs. NCBI nr
Match: gi|659067354|ref|XP_008439011.1| (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo])

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-116
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAEASGH 214
           CFSAFSVVSLFGFFFIDKRKAKKAAAAAEASGH
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAEASGH 213

[Close]
BLAST of MELO3C002305 vs. NCBI nr
Match: gi|525507162|ref|NP_001267619.1| (high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus])

HSP 1 Score: 387.9 bits (995), Expect = 1.2e-104
Identity = 193/208 (92.79%), Postives = 197/208 (94.71%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MASN FLLAALFLSCFLGFS GNIH SSLPRTLEVTASPKPGQVLKAGVDKI VTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQF IVEK YNPANKT QS
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV+RD+PTGTFFVRAYV NSAG+EVAYGQTTD KKGTNLFQIESITGRH+SLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAA 209
           CFSAFSVVSLFGFFFIDKRKAKKAAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAA 208

[Close]
BLAST of MELO3C002305 vs. NCBI nr
Match: gi|743905683|ref|XP_011046245.1| (PREDICTED: high-affinity nitrate transporter 3.1-like [Populus euphratica])

HSP 1 Score: 289.7 bits (740), Expect = 4.3e-75
Identity = 140/204 (68.63%), Postives = 166/204 (81.37%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MA+  FLLA+L LSCFL    G + LSSLPRTLEVTASP  GQVLK G DKI VTW LN 
Sbjct: 1   MAARAFLLASLVLSCFLLPCYGTVLLSSLPRTLEVTASPTSGQVLKGGEDKITVTWGLNQ 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           TV AG+DS+YK IK KLCYAP+SQVDR WRKT DDLKKD+TCQ  IV +PYNPAN TAQS
Sbjct: 61  TVAAGTDSTYKTIKVKLCYAPISQVDRGWRKTVDDLKKDRTCQHKIVARPYNPANSTAQS 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
            EWTVERDVPT T+F+RAY +++  +EVAYGQTTD  + TNLFQ+++I+GRHV++D  S+
Sbjct: 121 HEWTVERDVPTATYFIRAYAYDADEKEVAYGQTTDAGRTTNLFQVQAISGRHVTVDTCSI 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKA 205
           CFS FSVVSLFGFF+ +KR AK++
Sbjct: 181 CFSVFSVVSLFGFFYNEKRNAKRS 204

[Close]
BLAST of MELO3C002305 vs. NCBI nr
Match: gi|743905685|ref|XP_011046246.1| (PREDICTED: high-affinity nitrate transporter 3.1-like [Populus euphratica])

HSP 1 Score: 287.3 bits (734), Expect = 2.1e-74
Identity = 139/204 (68.14%), Postives = 165/204 (80.88%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MA+   LLA+L LSCFL    G + LSSLPRTLEVTASP  GQVLK G DKI VTW LN 
Sbjct: 1   MAARALLLASLVLSCFLLPCYGTVLLSSLPRTLEVTASPTSGQVLKGGEDKITVTWGLNQ 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           TV AG+DS+YK IK KLCYAP+SQVDR WRKT DDLKKD+TCQ  IV +PYNPAN TAQS
Sbjct: 61  TVAAGTDSTYKTIKVKLCYAPISQVDRGWRKTVDDLKKDRTCQHKIVARPYNPANSTAQS 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
            EWTVERDVPT T+F+RAY +++  +EVAYGQTTD  + TNLFQ+++I+GRHV++D  S+
Sbjct: 121 HEWTVERDVPTATYFIRAYAYDADEKEVAYGQTTDAGRTTNLFQVQAISGRHVTVDTCSI 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKA 205
           CFS FSVVSLFGFF+ +KR AK++
Sbjct: 181 CFSVFSVVSLFGFFYNEKRNAKRS 204

[Close]
BLAST of MELO3C002305 vs. NCBI nr
Match: gi|951070145|ref|XP_014490580.1| (PREDICTED: high-affinity nitrate transporter 3.1 [Vigna radiata var. radiata])

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-73
Identity = 133/207 (64.25%), Postives = 166/207 (80.19%), Query Frame = 1 

Query: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60
           MA++  ++ +L + CF G   G +H SSL +TL+VTASPK GQVL+AG DKI VTW LN 
Sbjct: 1   MAAHRLVVVSLLIFCFAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNN 60

Query: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120
           T+  G+DS+YK IK KLCYAP+SQ DRAWRKTED+L +DKTCQ  IV KPY+ +N+T Q 
Sbjct: 61  TLPTGTDSAYKTIKVKLCYAPISQKDRAWRKTEDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEW +ERDVP  T+FVRAY F+S G+EVAYGQTTD KK +NLF+I SI+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYAFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 208
           CFSAFSVV+LF FF+I+KRKAK ++++
Sbjct: 181 CFSAFSVVALFVFFYIEKRKAKASSSS 207

The following BLAST results are available for this feature:
BLAST of MELO3C002305 vs. Swiss-Prot
Match NameE-valueIdentityDescription
NRT31_ARATH5.7e-5158.38High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana GN=NRT3.1 PE=1 SV=... [more]
NRT32_ARATH2.0e-4849.28High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana GN=NRT3.2 PE=2 SV=... [more]
NAR21_ORYSJ2.2e-4247.22High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ... [more]
NAR22_ORYSJ2.6e-4048.62Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ... [more]
BLAST of MELO3C002305 vs. TrEMBL
Match NameE-valueIdentityDescription
C8CHG8_CUCSA8.1e-10592.79High-affinity nitrate transport system component OS=Cucumis sativus GN=NAR2 PE=2... [more]
A0A067K4F8_JATCU9.6e-7470.94Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11393 PE=4 SV=1[more]
A0A0S3R655_PHAAN6.2e-7363.77Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G397600 PE=... [more]
A0A0L9TQ82_PHAAN6.2e-7363.77Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g230400 PE=4 SV=1[more]
B9P5X4_POPTR1.8e-7267.65Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0015s09670g PE=4 SV=1[more]
BLAST of MELO3C002305 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G50200.33.2e-5258.38 nitrate transmembrane transporters[more]
AT4G24730.12.4e-4753.76 Calcineurin-like metallo-phosphoesterase superfamily protein[more]
BLAST of MELO3C002305 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659067354|ref|XP_008439011.1|1.3e-116100.00PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo][more]
gi|525507162|ref|NP_001267619.1|1.2e-10492.79high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus][more]
gi|743905683|ref|XP_011046245.1|4.3e-7568.63PREDICTED: high-affinity nitrate transporter 3.1-like [Populus euphratica][more]
gi|743905685|ref|XP_011046246.1|2.1e-7468.14PREDICTED: high-affinity nitrate transporter 3.1-like [Populus euphratica][more]
gi|951070145|ref|XP_014490580.1|1.1e-7364.25PREDICTED: high-affinity nitrate transporter 3.1 [Vigna radiata var. radiata][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016605Transptr_NO3_Nar2
Vocabulary: Biological Process
TermDefinition
GO:0010167response to nitrate
GO:0015706nitrate transport
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0003674 molecular_function
molecular_function GO:0015112 nitrate transmembrane transporter activity
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU64425melon EST collection version 4.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C002305T1MELO3C002305T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU64425MU64425transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016605High-affinity nitrate transporterPIRPIRSF012939NAR2coord: 1..205
score: 1.4
IPR016605High-affinity nitrate transporterPFAMPF16974NAR2coord: 26..201
score: 1.4
NoneNo IPR availablePANTHERPTHR34806FAMILY NOT NAMEDcoord: 1..207
score: 1.1E
NoneNo IPR availablePANTHERPTHR34806:SF1HIGH-AFFINITY NITRATE TRANSPORTER 3.1-RELATEDcoord: 1..207
score: 1.1E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
MELO3C002305Csa1G047430Cucumber (Chinese Long) v2cumeB027
MELO3C002305Cla005121Watermelon (97103) v1mewmB149
MELO3C002305ClCG03G003060Watermelon (Charleston Gray)mewcgB152
MELO3C002305Cla97C03G054140Watermelon (97103) v2mewmbB156
MELO3C002305Bhi08G001354Wax gourdmewgoB175
MELO3C002305CSPI01G08880Wild cucumber (PI 183967)cpimeB024
MELO3C002305Cucsa.098830Cucumber (Gy14) v1cgymeB116
MELO3C002305CmaCh13G007600Cucurbita maxima (Rimu)cmameB197
MELO3C002305CmaCh18G001960Cucurbita maxima (Rimu)cmameB365
MELO3C002305CmoCh18G001580Cucurbita moschata (Rifu)cmomeB356
MELO3C002305CmoCh13G007900Cucurbita moschata (Rifu)cmomeB183
MELO3C002305Lsi02G004230Bottle gourd (USVL1VR-Ls)lsimeB179
MELO3C002305Cp4.1LG09g10080Cucurbita pepo (Zucchini)cpemeB026
MELO3C002305Cp4.1LG20g03470Cucurbita pepo (Zucchini)cpemeB470
MELO3C002305CsGy1G008810Cucumber (Gy14) v2cgybmeB022
MELO3C002305Carg07560Silver-seed gourdcarmeB0978
MELO3C002305Carg06757Silver-seed gourdcarmeB0499
MELO3C002305CsaV3_1G008910Cucumber (Chinese Long) v3cucmeB024
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
MELO3C002305Cucurbita maxima (Rimu)cmameB051
MELO3C002305Cucurbita maxima (Rimu)cmameB088
MELO3C002305Cucurbita moschata (Rifu)cmomeB041
MELO3C002305Wild cucumber (PI 183967)cpimeB015
MELO3C002305Cucumber (Chinese Long) v2cumeB019
MELO3C002305Cucumber (Chinese Long) v2cumeB386
MELO3C002305Watermelon (Charleston Gray)mewcgB158
MELO3C002305Watermelon (Charleston Gray)mewcgB160
MELO3C002305Watermelon (Charleston Gray)mewcgB161
MELO3C002305Watermelon (97103) v1mewmB155
MELO3C002305Cucurbita pepo (Zucchini)cpemeB603
MELO3C002305Bottle gourd (USVL1VR-Ls)lsimeB289
MELO3C002305Bottle gourd (USVL1VR-Ls)lsimeB392
MELO3C002305Bottle gourd (USVL1VR-Ls)lsimeB465
MELO3C002305Watermelon (97103) v2mewmbB163
MELO3C002305Watermelon (97103) v2mewmbB165
MELO3C002305Watermelon (97103) v2mewmbB168
MELO3C002305Wax gourdmewgoB189
MELO3C002305Melon (DHL92) v3.5.1memeB067
MELO3C002305Melon (DHL92) v3.5.1memeB068