CmoCh13G007900 (gene) Cucurbita moschata (Rifu)

NameCmoCh13G007900
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionHigh-affinity nitrate transport system component
LocationCmo_Chr13 : 7530119 .. 7531250 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGTTCTGTTTCTTGGGATTCTCTCATGGACATATCCATTTCTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCACCTAAACCTGGACAAGGTACGATTTTTCAGCTCCACTGTTAGATCTCTTGTAACATTTCCCTAATACACGCATTGGGTAAACCTTGAGAAAGCCAAAATGGAGAATATCTACGTTGATGAGCTTGGACAGTTACAAATCAAACACCAGGCGATGTGTCAACGATAAGGCTGAGTCTCGAAGAAGGATGGACACAAGATCGAATGGGGATGGATAGGAGGCAGTGTGACAACAAGGACACTGGATCCATCGAGTTGTAAAAATTTGAAAGGGAAAACATATCCACTAGCTGTGTGCTTGATCTGAATCCAAAATTGGAACTTTACCCATTTCTAACAACTGGGTTGTCTTTTTCTTTCTGCAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGTCAAGTGGACCGGGCCTGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGGGAACAAGACGGTACAAAAGTTCGAGTGGACGGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGATGAAGTGGGCTACGGGCAAACGACTGACAACAAAAAAGGCACGAACTTGTTTCAAATCGAAGCGATCACAGGTCGCCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTAGCCGGCAAGAGAAAGGCCAAGAAGGCGGAGGCGCCGGGACAGTAAGTTAACAAAGATGGAAACTTGCTCTGCTTCTGAGTTCTTTTTTTTAAGTCTTTCTGCATTTTGGATTTCTCTTGTACTGTAATTTTCTAGGTGAAAAGATGGAAAATAAAGTGTATTAAATTATGAATGTGTC

mRNA sequence

GAAATATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGTTCTGTTTCTTGGGATTCTCTCATGGACATATCCATTTCTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCACCTAAACCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGTCAAGTGGACCGGGCCTGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGGGAACAAGACGGTACAAAAGTTCGAGTGGACGGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGATGAAGTGGGCTACGGGCAAACGACTGACAACAAAAAAGGCACGAACTTGTTTCAAATCGAAGCGATCACAGGTCGCCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTAGCCGGCAAGAGAAAGGCCAAGAAGGCGGAGGCGCCGGGACAGTAAGTTAACAAAGATGGAAACTTGCTCTGCTTCTGAGTTCTTTTTTTTAAGTCTTTCTGCATTTTGGATTTCTCTTGTACTGTAATTTTCTAGGTGAAAAGATGGAAAATAAAGTGTATTAAATTATGAATGTGTC

Coding sequence (CDS)

ATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGTTCTGTTTCTTGGGATTCTCTCATGGACATATCCATTTCTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCACCTAAACCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGTCAAGTGGACCGGGCCTGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGGGAACAAGACGGTACAAAAGTTCGAGTGGACGGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGATGAAGTGGGCTACGGGCAAACGACTGACAACAAAAAAGGCACGAACTTGTTTCAAATCGAAGCGATCACAGGTCGCCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTAGCCGGCAAGAGAAAGGCCAAGAAGGCGGAGGCGCCGGGACAGTAA
BLAST of CmoCh13G007900 vs. Swiss-Prot
Match: NRT31_ARATH (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.4e-49
Identity = 106/209 (50.72%), Postives = 141/209 (67.46%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIH---FSSLPR-SLEVTASPK---PGQVLKAGVDKIS 66
           M+    L A+L +   +   HG      F  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 67  VTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 126
           +TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC   I+ KPY+ 
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPYD- 120

Query: 127 GNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHI 186
             KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNLF ++AI+GRH 
Sbjct: 121 --KTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 187 SLDIASVCFSAFSVLSLSGFFLAGKRKAK 209
           SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

BLAST of CmoCh13G007900 vs. Swiss-Prot
Match: NRT32_ARATH (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.2e-47
Identity = 101/207 (48.79%), Postives = 137/207 (66.18%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIH---FSSLPRSLEVTASP-KPGQVLKAGVDKISVTW 66
           M+ +  L  +L +F  +  S G      F+ L  S+EVTA P K   VL+AG D +++TW
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 67  VL-NGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGN 126
            L + + K  +D+++KT++ KLCYAP+SQVDR WRKT++ L KD++C   IV K Y+   
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAYD--- 120

Query: 127 KTVQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISL 186
           KT Q  +WT+  DIPTGT+FVRAY  +  G EV YGQ+TD  + TNLF + AI+G H+ L
Sbjct: 121 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 180

Query: 187 DIASVCFSAFSVLSLSGFFLAGKRKAK 209
           DIAS  FS FSV+SL  FF+  KRKAK
Sbjct: 181 DIASTFFSVFSVVSLFVFFVMEKRKAK 204

BLAST of CmoCh13G007900 vs. Swiss-Prot
Match: NAR21_ORYSJ (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.4e-41
Identity = 84/180 (46.67%), Postives = 121/180 (67.22%), Query Frame = 1

Query: 30  IHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVS 89
           +  S LP++L V  SPK G+V+ AG + ++VTW LN +  +G+D+++K+V+ KLCYAP S
Sbjct: 34  VFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPAS 93

Query: 90  QVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRAYAFNS 149
           + DR WRK  DDL KDK CQF +  +PY  G     +F++ VARDIPT ++FVRAYA ++
Sbjct: 94  RTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDA 153

Query: 150 AGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKK 209
           +G EV YGQ++ +      F +  ITG H SL +A+  FS FS+ +L+ FF+  KRK  K
Sbjct: 154 SGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

BLAST of CmoCh13G007900 vs. Swiss-Prot
Match: NAR22_ORYSJ (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 6.5e-39
Identity = 92/206 (44.66%), Postives = 122/206 (59.22%), Query Frame = 1

Query: 14  LAALFLFCFLGFSH-------GHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 73
           L  L L   LG  H            S+L ++L V ASP+ GQVL AG D I+VTW LN 
Sbjct: 13  LPLLLLLVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAGEDAITVTWSLNA 72

Query: 74  TVKS---GSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKT 133
           T  +   G+D+ YK V+  LCYAP SQV R WRK  DDLSKDK CQF I  +PY+     
Sbjct: 73  TAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFKIAQQPYDGAG-- 132

Query: 134 VQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDI 193
             KFE+TVARD+PT +++VRAYA +++G  V YG+T  +      F +  ITG   S+++
Sbjct: 133 --KFEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS----FAVAGITGVTASIEV 192

Query: 194 ASVCFSAFSVLSLSGFFLAGKRKAKK 210
           A+   SAFSV +L+ F +   +K  K
Sbjct: 193 AAGVLSAFSVAALAVFLVLENKKKNK 210

BLAST of CmoCh13G007900 vs. TrEMBL
Match: C8CHG8_CUCSA (High-affinity nitrate transport system component OS=Cucumis sativus GN=NAR2 PE=2 SV=2)

HSP 1 Score: 355.1 bits (910), Expect = 5.8e-95
Identity = 178/211 (84.36%), Postives = 188/211 (89.10%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++N FLLAALFL CFLGFSHG+IHFSSLPR+LEVTASPKPGQVLKAGVDKISVTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV K YNP NKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEWTV RDIPTGTFFVRAY  NSAG+EV YGQTTD KKGTNLFQIE+ITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKK--AEAPGQ 216
           CFSAFSV+SL GFF   KRKAKK  A APGQ
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAPGQ 211

BLAST of CmoCh13G007900 vs. TrEMBL
Match: V7CBE9_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G205900g PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 1.7e-70
Identity = 128/202 (63.37%), Postives = 162/202 (80.20%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+G ++A+L +FC  G  +G +HFSSL  +L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHGLVVASLLIFCLAGSCYGKVHFSSLKNTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           TV +G+DS+YKT++ KLCYAP+SQ DRAWRKTE++LS+DKTCQ  IV KPY+  NKTVQ+
Sbjct: 61  TVSTGADSAYKTIKVKLCYAPISQKDRAWRKTEEELSRDKTCQHKIVAKPYDASNKTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEW + +DIP  T+FVRAYAFNS  +EV +GQTTD KK +NLF I +I+GRH SLDI S+
Sbjct: 121 FEWVIEKDIPKATYFVRAYAFNSNDEEVAFGQTTDAKKSSNLFDINSISGRHASLDICSI 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAK 209
           CFSAFSV+SL  FF   KR+ K
Sbjct: 181 CFSAFSVVSLFVFFYIEKRQGK 202

BLAST of CmoCh13G007900 vs. TrEMBL
Match: A0A0L9TQ82_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g230400 PE=4 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.8e-70
Identity = 127/206 (61.65%), Postives = 164/206 (79.61%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+  ++A+L +FC  G  +G +HFSSL ++L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHSLVVASLLIFCLAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           T+ SG+DS+YKT++ KLCYAP+SQ DRAWRKT D+LS+DKTCQ  IV KPY+  N+TVQ+
Sbjct: 61  TLPSGTDSAYKTIKVKLCYAPISQKDRAWRKTVDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEW + RD+P  T+FVRAY F+S G+EV YGQTTD KK +NLF+I +I+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYVFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 213
           CFSAFSV++L  FF   KRK K + +
Sbjct: 181 CFSAFSVVALFVFFYIEKRKGKASSS 206

BLAST of CmoCh13G007900 vs. TrEMBL
Match: A0A0S3R655_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G397600 PE=4 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.8e-70
Identity = 127/206 (61.65%), Postives = 164/206 (79.61%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+  ++A+L +FC  G  +G +HFSSL ++L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHSLVVASLLIFCLAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           T+ SG+DS+YKT++ KLCYAP+SQ DRAWRKT D+LS+DKTCQ  IV KPY+  N+TVQ+
Sbjct: 61  TLPSGTDSAYKTIKVKLCYAPISQKDRAWRKTVDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEW + RD+P  T+FVRAY F+S G+EV YGQTTD KK +NLF+I +I+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYVFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 213
           CFSAFSV++L  FF   KRK K + +
Sbjct: 181 CFSAFSVVALFVFFYIEKRKGKASSS 206

BLAST of CmoCh13G007900 vs. TrEMBL
Match: A0A067K4F8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11393 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 5.0e-70
Identity = 137/205 (66.83%), Postives = 159/205 (77.56%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+  G  LA++ LF F G S+G IHFSSL ++L VTASP PGQVLKAGVD I+VTW LN 
Sbjct: 1   MAPRGLFLASILLFAFFGSSYGVIHFSSLQKTLLVTASPSPGQVLKAGVDNITVTWGLNQ 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           ++ +G+DS+YKT++ KLCYAPVSQ DRAWRKTED+LSKDKTCQ  IV KPY+  NK    
Sbjct: 61  SLPAGTDSAYKTIKIKLCYAPVSQKDRAWRKTEDNLSKDKTCQVKIVTKPYSSANKN-NS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           F WTV RD+PT T+FVRAYAFN+   EV YGQTTD  K TN F I+AITGRH SLDIASV
Sbjct: 121 FTWTVERDVPTATYFVRAYAFNANEQEVAYGQTTDVHKTTNFFDIQAITGRHASLDIASV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKKAE 212
           CFS FSV++L GFF   KRKAKK E
Sbjct: 181 CFSVFSVVALFGFFYIEKRKAKKTE 204

BLAST of CmoCh13G007900 vs. TAIR10
Match: AT5G50200.3 (AT5G50200.3 nitrate transmembrane transporters)

HSP 1 Score: 196.8 bits (499), Expect = 1.3e-50
Identity = 106/209 (50.72%), Postives = 141/209 (67.46%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIH---FSSLPR-SLEVTASPK---PGQVLKAGVDKIS 66
           M+    L A+L +   +   HG      F  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 67  VTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 126
           +TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC   I+ KPY+ 
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPYD- 120

Query: 127 GNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHI 186
             KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNLF ++AI+GRH 
Sbjct: 121 --KTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 187 SLDIASVCFSAFSVLSLSGFFLAGKRKAK 209
           SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

BLAST of CmoCh13G007900 vs. TAIR10
Match: AT4G24730.1 (AT4G24730.1 Calcineurin-like metallo-phosphoesterase superfamily protein)

HSP 1 Score: 181.8 bits (460), Expect = 4.5e-46
Identity = 95/184 (51.63%), Postives = 124/184 (67.39%), Query Frame = 1

Query: 26  SHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVL-NGTVKSGSDSSYKTVEAKLC 85
           SHG  H     RSLE      P  VL+AG D +++TW L + + K  +D+++KT++ KLC
Sbjct: 265 SHGVHH-----RSLEAALECPP--VLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLC 324

Query: 86  YAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRA 145
           YAP+SQVDR WRKT++ L KD++C   IV K Y+   KT Q  +WT+  DIPTGT+FVRA
Sbjct: 325 YAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAYD---KTPQSLDWTIGLDIPTGTYFVRA 384

Query: 146 YAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASVCFSAFSVLSLSGFFLAGK 205
           Y  +  G EV YGQ+TD  + TNLF + AI+G H+ LDIAS  FS FSV+SL  FF+  K
Sbjct: 385 YGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEK 438

Query: 206 RKAK 209
           RKAK
Sbjct: 445 RKAK 438

BLAST of CmoCh13G007900 vs. NCBI nr
Match: gi|525507162|ref|NP_001267619.1| (high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus])

HSP 1 Score: 355.1 bits (910), Expect = 8.4e-95
Identity = 178/211 (84.36%), Postives = 188/211 (89.10%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++N FLLAALFL CFLGFSHG+IHFSSLPR+LEVTASPKPGQVLKAGVDKISVTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV K YNP NKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEWTV RDIPTGTFFVRAY  NSAG+EV YGQTTD KKGTNLFQIE+ITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKK--AEAPGQ 216
           CFSAFSV+SL GFF   KRKAKK  A APGQ
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAPGQ 211

BLAST of CmoCh13G007900 vs. NCBI nr
Match: gi|659067354|ref|XP_008439011.1| (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo])

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-94
Identity = 174/209 (83.25%), Postives = 187/209 (89.47%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++NGFLLAALFL CFLGFS G+IH SSLPR+LEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV KPYNP NKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEWTV RD+PTGTFFVRAY FNSAG EV YGQTTD+KKGTNLFQIE+ITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 216
           CFSAFSV+SL GFF   KRKAKKA A  +
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAE 209

BLAST of CmoCh13G007900 vs. NCBI nr
Match: gi|951070145|ref|XP_014490580.1| (PREDICTED: high-affinity nitrate transporter 3.1 [Vigna radiata var. radiata])

HSP 1 Score: 278.1 bits (710), Expect = 1.3e-71
Identity = 129/206 (62.62%), Postives = 167/206 (81.07%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+  ++ +L +FCF G  +G +HFSSL ++L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHRLVVVSLLIFCFAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNN 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           T+ +G+DS+YKT++ KLCYAP+SQ DRAWRKTED+LS+DKTCQ  IV KPY+  N+TVQ+
Sbjct: 61  TLPTGTDSAYKTIKVKLCYAPISQKDRAWRKTEDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEW + RD+P  T+FVRAYAF+S G+EV YGQTTD KK +NLF+I +I+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYAFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 213
           CFSAFSV++L  FF   KRKAK + +
Sbjct: 181 CFSAFSVVALFVFFYIEKRKAKASSS 206

BLAST of CmoCh13G007900 vs. NCBI nr
Match: gi|1009130794|ref|XP_015882491.1| (PREDICTED: high-affinity nitrate transporter 3.1 [Ziziphus jujuba])

HSP 1 Score: 274.2 bits (700), Expect = 1.9e-70
Identity = 138/206 (66.99%), Postives = 162/206 (78.64%), Query Frame = 1

Query: 7   MSANGFLLAALFLF-CFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLN 66
           M+A G LLA+L LF CF   SHG +HFSSL R+L VTASPKPGQVLKAG DKI+V W LN
Sbjct: 1   MAARGVLLASLLLFSCFAEISHGKVHFSSLQRTLVVTASPKPGQVLKAGEDKITVNWGLN 60

Query: 67  GTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQ 126
            T  +G DS+YKT++ KLCYAP+SQ DRAWRKTED +SKDKTCQF IV +PY+  N   Q
Sbjct: 61  KTFPAGVDSAYKTIKVKLCYAPISQADRAWRKTEDLISKDKTCQFKIVARPYSASN---Q 120

Query: 127 KFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIAS 186
            FEWTV RDIP  T+F+RAYA+ S   EV YGQTTD  K TNLF ++A+TGRH SLDIAS
Sbjct: 121 SFEWTVERDIPLATYFIRAYAYTSEEVEVAYGQTTDAHKTTNLFDVQAVTGRHASLDIAS 180

Query: 187 VCFSAFSVLSLSGFFLAGKRKAKKAE 212
           VCFSAFSV+SL+ FF+A KRKAK ++
Sbjct: 181 VCFSAFSVVSLAVFFIAEKRKAKSSQ 203

BLAST of CmoCh13G007900 vs. NCBI nr
Match: gi|593784899|ref|XP_007155490.1| (hypothetical protein PHAVU_003G205900g [Phaseolus vulgaris])

HSP 1 Score: 273.9 bits (699), Expect = 2.5e-70
Identity = 128/202 (63.37%), Postives = 162/202 (80.20%), Query Frame = 1

Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+G ++A+L +FC  G  +G +HFSSL  +L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHGLVVASLLIFCLAGSCYGKVHFSSLKNTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           TV +G+DS+YKT++ KLCYAP+SQ DRAWRKTE++LS+DKTCQ  IV KPY+  NKTVQ+
Sbjct: 61  TVSTGADSAYKTIKVKLCYAPISQKDRAWRKTEEELSRDKTCQHKIVAKPYDASNKTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEW + +DIP  T+FVRAYAFNS  +EV +GQTTD KK +NLF I +I+GRH SLDI S+
Sbjct: 121 FEWVIEKDIPKATYFVRAYAFNSNDEEVAFGQTTDAKKSSNLFDINSISGRHASLDICSI 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAK 209
           CFSAFSV+SL  FF   KR+ K
Sbjct: 181 CFSAFSVVSLFVFFYIEKRQGK 202

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NRT31_ARATH2.4e-4950.72High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana GN=NRT3.1 PE=1 SV=... [more]
NRT32_ARATH2.2e-4748.79High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana GN=NRT3.2 PE=2 SV=... [more]
NAR21_ORYSJ1.4e-4146.67High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ... [more]
NAR22_ORYSJ6.5e-3944.66Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ... [more]
Match NameE-valueIdentityDescription
C8CHG8_CUCSA5.8e-9584.36High-affinity nitrate transport system component OS=Cucumis sativus GN=NAR2 PE=2... [more]
V7CBE9_PHAVU1.7e-7063.37Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G205900g PE=4 SV=1[more]
A0A0L9TQ82_PHAAN3.8e-7061.65Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g230400 PE=4 SV=1[more]
A0A0S3R655_PHAAN3.8e-7061.65Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G397600 PE=... [more]
A0A067K4F8_JATCU5.0e-7066.83Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11393 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G50200.31.3e-5050.72 nitrate transmembrane transporters[more]
AT4G24730.14.5e-4651.63 Calcineurin-like metallo-phosphoesterase superfamily protein[more]
Match NameE-valueIdentityDescription
gi|525507162|ref|NP_001267619.1|8.4e-9584.36high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus][more]
gi|659067354|ref|XP_008439011.1|1.1e-9483.25PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo][more]
gi|951070145|ref|XP_014490580.1|1.3e-7162.62PREDICTED: high-affinity nitrate transporter 3.1 [Vigna radiata var. radiata][more]
gi|1009130794|ref|XP_015882491.1|1.9e-7066.99PREDICTED: high-affinity nitrate transporter 3.1 [Ziziphus jujuba][more]
gi|593784899|ref|XP_007155490.1|2.5e-7063.37hypothetical protein PHAVU_003G205900g [Phaseolus vulgaris][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016605Transptr_NO3_Nar2
Vocabulary: Biological Process
TermDefinition
GO:0010167response to nitrate
GO:0015706nitrate transport
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G007900.1CmoCh13G007900.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016605High-affinity nitrate transporterPIRPIRSF012939NAR2coord: 7..211
score: 3.5
IPR016605High-affinity nitrate transporterPFAMPF16974NAR2coord: 32..207
score: 4.0
NoneNo IPR availablePANTHERPTHR34806FAMILY NOT NAMEDcoord: 4..213
score: 1.1E
NoneNo IPR availablePANTHERPTHR34806:SF1HIGH-AFFINITY NITRATE TRANSPORTER 3.1-RELATEDcoord: 4..213
score: 1.1E