CmoCh18G001580 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh18G001580
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	High-affinity nitrate transporter 3.2-like protein
Location	Cmo_Chr18 : 1098992 .. 1100177 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCTCTTCTGTCTCTTCATTGCACTTTAGAGCCAGAGCTCCCCTGTTTCTTAATATTCAAAGAATATTCATTCTTCCTCAAATTCTCATCCCCAACTTGGCCTCTTTTGACTTTTTCCCTCTTAATCTCTATTTAAACCAAACCAATCCCAATTTCTGCCATTAACTTCATCAAATCTGAGATTCTTCTCTGAAACTTGAGAGCTTAAACCTTAAATCAACAATGGCCGCTCATGGGTTTCCTCTGACTGCTCTGTTTTTGCTCTGTTTCTTGGGGTTCTCTCATGGACATATCCATCTTTCTTCTATCCCAAGAAATTTGGAGGTCACTGCTTCTCCAAAATCTGGACAAGGTAAGAACTTTCAGCTCCCATAACTCAAATTTCTGATGCTTCCAACACAGATCTGAATAAAAAACTGTTAGTTTAGCGATTTTTAACATCTGGGTTTTTGTTTTTTTTTGCAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGTCATGGGTTCTTAATGGAGCTGCAAAAGTTGGGTCGGATTTGGCTTACAAATCTGTGAAGGTGAAGCTCTGCTATGCGCCGGTGAGCCAAGTGGACCGTGCGTGGAGAAAAACAGAGGACGATCTTGCAAAGGACAAGACTTGTCAATTCCAAATCGTGGAGAAGCCGTACAATCCGTCGAACAAGACGGTTCAAAAGGCTGAGTGGACGGTGGAGCGTGACACTCCGACGGCGACTTTCTTCGTGCGTGCTTACGCGTTCAATTCCGCCGGCGATGAAGTGGCGTACGGACAAACGACAGACGAAAAGAAAGGCACAAATTTGTTCCAAGTTGAAGCTATCACTGGCCGCCATATCTCTCTCGATATTGCTTCTGGTTGTTTCAGTGCTTTCTCTGTGCTGTCTTTGACCGGATTCTTCATCGCCGGTAAGAGGCAGGCGAAGAAGGCGGCGGCGGCGGAGGGGCACTAAGTGGGAACTTTGCTCTGTTTTGAGGTTCCCTTTTAAAGCTTTGCATCAATGGCGATCTGCTTGGTGAAGAGTAGATTTTATGAGTTCTTTTTTTTAGTCTTTTTGTGATTTGTGTTTCTCTTGTACTATAATTTTATGATATATAATATGATATTAATGTGTCTAATGTTTGATGTGAAAAATTTAAATGAAAATATTTGAAAATTTGGACTTTGGGG

mRNA sequence

TCCTCTTCTGTCTCTTCATTGCACTTTAGAGCCAGAGCTCCCCTGTTTCTTAATATTCAAAGAATATTCATTCTTCCTCAAATTCTCATCCCCAACTTGGCCTCTTTTGACTTTTTCCCTCTTAATCTCTATTTAAACCAAACCAATCCCAATTTCTGCCATTAACTTCATCAAATCTGAGATTCTTCTCTGAAACTTGAGAGCTTAAACCTTAAATCAACAATGGCCGCTCATGGGTTTCCTCTGACTGCTCTGTTTTTGCTCTGTTTCTTGGGGTTCTCTCATGGACATATCCATCTTTCTTCTATCCCAAGAAATTTGGAGGTCACTGCTTCTCCAAAATCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGTCATGGGTTCTTAATGGAGCTGCAAAAGTTGGGTCGGATTTGGCTTACAAATCTGTGAAGGTGAAGCTCTGCTATGCGCCGGTGAGCCAAGTGGACCGTGCGTGGAGAAAAACAGAGGACGATCTTGCAAAGGACAAGACTTGTCAATTCCAAATCGTGGAGAAGCCGTACAATCCGTCGAACAAGACGGTTCAAAAGGCTGAGTGGACGGTGGAGCGTGACACTCCGACGGCGACTTTCTTCGTGCGTGCTTACGCGTTCAATTCCGCCGGCGATGAAGTGGCGTACGGACAAACGACAGACGAAAAGAAAGGCACAAATTTGTTCCAAGTTGAAGCTATCACTGGCCGCCATATCTCTCTCGATATTGCTTCTGGTTGTTTCAGTGCTTTCTCTGTGCTGTCTTTGACCGGATTCTTCATCGCCGGTAAGAGGCAGGCGAAGAAGGCGGCGGCGGCGGAGGGGCACTAAGTGGGAACTTTGCTCTGTTTTGAGGTTCCCTTTTAAAGCTTTGCATCAATGGCGATCTGCTTGGTGAAGAGTAGATTTTATGAGTTCTTTTTTTTAGTCTTTTTGTGATTTGTGTTTCTCTTGTACTATAATTTTATGATATATAATATGATATTAATGTGTCTAATGTTTGATGTGAAAAATTTAAATGAAAATATTTGAAAATTTGGACTTTGGGG

Coding sequence (CDS)

ATGGCCGCTCATGGGTTTCCTCTGACTGCTCTGTTTTTGCTCTGTTTCTTGGGGTTCTCTCATGGACATATCCATCTTTCTTCTATCCCAAGAAATTTGGAGGTCACTGCTTCTCCAAAATCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGTCATGGGTTCTTAATGGAGCTGCAAAAGTTGGGTCGGATTTGGCTTACAAATCTGTGAAGGTGAAGCTCTGCTATGCGCCGGTGAGCCAAGTGGACCGTGCGTGGAGAAAAACAGAGGACGATCTTGCAAAGGACAAGACTTGTCAATTCCAAATCGTGGAGAAGCCGTACAATCCGTCGAACAAGACGGTTCAAAAGGCTGAGTGGACGGTGGAGCGTGACACTCCGACGGCGACTTTCTTCGTGCGTGCTTACGCGTTCAATTCCGCCGGCGATGAAGTGGCGTACGGACAAACGACAGACGAAAAGAAAGGCACAAATTTGTTCCAAGTTGAAGCTATCACTGGCCGCCATATCTCTCTCGATATTGCTTCTGGTTGTTTCAGTGCTTTCTCTGTGCTGTCTTTGACCGGATTCTTCATCGCCGGTAAGAGGCAGGCGAAGAAGGCGGCGGCGGCGGAGGGGCACTAA

Homology

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BLAST of CmoCh18G001580 vs. Swiss-Prot
Match: NRT31_ARATH (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 6.3e-47
Identity = 99/175 (56.57%), Postives = 126/175 (72.00%), Query Frame = 1


Query: 33  LEVTASPKS---GQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDR 92
           L+VT  P     G VL AG D ++++W L+    +GS  +  +K +KVKLCYAP SQVDR
Sbjct: 37  LDVTTKPSREGPGVVLDAGKDTLNITWTLSS---IGSKREAEFKIIKVKLCYAPPSQVDR 96

Query: 93  AWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDE 152
            WRKT D+L KDKTC  +I+ KPY+   KT+Q   WT+ERD PT T+FVRAYA ++ G E
Sbjct: 97  PWRKTHDELFKDKTCPHKIIAKPYD---KTLQSTTWTLERDIPTGTYFVRAYAVDAIGHE 156

Query: 153 VAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 203
           VAYGQ+TD+ K TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 157 VAYGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of CmoCh18G001580 vs. Swiss-Prot
Match: NRT32_ARATH (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 9.2e-46
Identity = 100/207 (48.31%), Postives = 137/207 (66.18%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIH---LSSIPRNLEVTASP-KSGQVLKAGVDKISVSW 60
           MA H     +L +   +  S G       + +  ++EVTA P K   VL+AG D ++++W
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 61  VL-NGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSN 120
            L + +AKV +D A+K+++VKLCYAP+SQVDR WRKT++ L KD++C  +IV K Y+   
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAYD--- 120

Query: 121 KTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISL 180
           KT Q  +WT+  D PT T+FVRAY  +  G EVAYGQ+TDE + TNLF V AI+G H+ L
Sbjct: 121 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 180

Query: 181 DIASGCFSAFSVLSLTGFFIAGKRQAK 203
           DIAS  FS FSV+SL  FF+  KR+AK
Sbjct: 181 DIASTFFSVFSVVSLFVFFVMEKRKAK 204

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BLAST of CmoCh18G001580 vs. Swiss-Prot
Match: NAR21_ORYSJ (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-41
Identity = 86/180 (47.78%), Postives = 119/180 (66.11%), Query Frame = 1


Query: 24  IHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVS 83
           + LS +P+ L V  SPK G+V+ AG + ++V+W LN +   G+D A+KSVKVKLCYAP S
Sbjct: 34  VFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPAS 93

Query: 84  QVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNS 143
           + DR WRK  DDL KDK CQF++  +PY        + ++ V RD PTA++FVRAYA ++
Sbjct: 94  RTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDA 153

Query: 144 AGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKK 203
           +G EVAYGQ++ +      F V  ITG H SL +A+G FS FS+ +L  FF+  KR+  K
Sbjct: 154 SGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

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BLAST of CmoCh18G001580 vs. Swiss-Prot
Match: NAR22_ORYSJ (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 5.8e-40
Identity = 96/210 (45.71%), Postives = 127/210 (60.48%), Query Frame = 1


Query: 4   HGFPLTALFLLCFLGFSH-------GHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSW 63
           H   L  L LL  LG  H           LS++ + L V ASP++GQVL AG D I+V+W
Sbjct: 9   HRVFLPLLLLLVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAGEDAITVTW 68

Query: 64  VLNG---AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNP 123
            LN    AA  G+D  YK+VKV LCYAP SQV R WRK  DDL+KDK CQF+I ++PY+ 
Sbjct: 69  SLNATAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFKIAQQPYDG 128

Query: 124 SNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHI 183
           +     K E+TV RD PTA+++VRAYA +++G  VAYG+T         F V  ITG   
Sbjct: 129 AG----KFEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS----FAVAGITGVTA 188

Query: 184 SLDIASGCFSAFSVLSLTGFFIAGKRQAKK 204
           S+++A+G  SAFSV +L  F +   ++  K
Sbjct: 189 SIEVAAGVLSAFSVAALAVFLVLENKKKNK 210

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BLAST of CmoCh18G001580 vs. TrEMBL
Match: C8CHG8_CUCSA (High-affinity nitrate transport system component OS=Cucumis sativus GN=NAR2 PE=2 SV=2)

HSP 1 Score: 331.3 bits (848), Expect = 8.8e-88
Identity = 164/207 (79.23%), Postives = 179/207 (86.47%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MA++ F L ALFL CFLGFSHG+IH SS+PR LEVTASPK GQVLKAGVDKISV+WVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
             K GSD +YK++K KLCYAPVSQVDRAWRKTEDDL KDKTCQF IVEK YNP+NKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EWTV+RD PT TFFVRAY  NSAG+EVAYGQTTD KKGTNLFQ+E+ITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAA 208
           CFSAFSV+SL GFF   KR+AKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

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BLAST of CmoCh18G001580 vs. TrEMBL
Match: V7CBE9_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G205900g PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 2.9e-67
Identity = 125/205 (60.98%), Postives = 158/205 (77.07%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAHG  + +L + C  G  +G +H SS+   L+VTASPK GQVL+AG DKI+V+W LN 
Sbjct: 1   MAAHGLVVASLLIFCLAGSCYGKVHFSSLKNTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
               G+D AYK++KVKLCYAP+SQ DRAWRKTE++L++DKTCQ +IV KPY+ SNKTVQ+
Sbjct: 61  TVSTGADSAYKTIKVKLCYAPISQKDRAWRKTEEELSRDKTCQHKIVAKPYDASNKTVQR 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EW +E+D P AT+FVRAYAFNS  +EVA+GQTTD KK +NLF + +I+GRH SLDI S 
Sbjct: 121 FEWVIEKDIPKATYFVRAYAFNSNDEEVAFGQTTDAKKSSNLFDINSISGRHASLDICSI 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAA 206
           CFSAFSV+SL  FF   KRQ K ++
Sbjct: 181 CFSAFSVVSLFVFFYIEKRQGKASS 205

[Close]

BLAST of CmoCh18G001580 vs. TrEMBL
Match: A0A0L9TQ82_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g230400 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 4.3e-66
Identity = 122/208 (58.65%), Postives = 161/208 (77.40%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAH   + +L + C  G  +G +H SS+ + L+VTASPK GQVL+AG DKI+V+W LN 
Sbjct: 1   MAAHSLVVASLLIFCLAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
               G+D AYK++KVKLCYAP+SQ DRAWRKT D+L++DKTCQ +IV KPY+ SN+TVQ+
Sbjct: 61  TLPSGTDSAYKTIKVKLCYAPISQKDRAWRKTVDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EW +ERD P AT+FVRAY F+S G+EVAYGQTTD KK +NLF++ +I+GRH SLDI S 
Sbjct: 121 FEWVIERDVPKATYFVRAYVFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAE 209
           CFSAFSV++L  FF   KR+ K +++++
Sbjct: 181 CFSAFSVVALFVFFYIEKRKGKASSSSK 208

[Close]

BLAST of CmoCh18G001580 vs. TrEMBL
Match: A0A0S3R655_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G397600 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 4.3e-66
Identity = 122/208 (58.65%), Postives = 161/208 (77.40%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAH   + +L + C  G  +G +H SS+ + L+VTASPK GQVL+AG DKI+V+W LN 
Sbjct: 1   MAAHSLVVASLLIFCLAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
               G+D AYK++KVKLCYAP+SQ DRAWRKT D+L++DKTCQ +IV KPY+ SN+TVQ+
Sbjct: 61  TLPSGTDSAYKTIKVKLCYAPISQKDRAWRKTVDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EW +ERD P AT+FVRAY F+S G+EVAYGQTTD KK +NLF++ +I+GRH SLDI S 
Sbjct: 121 FEWVIERDVPKATYFVRAYVFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAE 209
           CFSAFSV++L  FF   KR+ K +++++
Sbjct: 181 CFSAFSVVALFVFFYIEKRKGKASSSSK 208

[Close]

BLAST of CmoCh18G001580 vs. TrEMBL
Match: A0A151QX36_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_044187 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 3.6e-65
Identity = 126/206 (61.17%), Postives = 157/206 (76.21%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           M A    + +L + C  G  +G +H SS+ R L+VTASPK GQVL AGVDKI+V+W LN 
Sbjct: 1   MVALRLVVASLLIFCLAGSCYGKVHFSSLKRTLDVTASPKHGQVLVAGVDKITVTWALNK 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
               G+D AYK++K+KLCYAP+SQ DRAWRKTED+L++DKTCQ +IV KPY+ SNKTVQ+
Sbjct: 61  TLPAGTDSAYKTIKLKLCYAPISQRDRAWRKTEDELSRDKTCQHKIVAKPYDASNKTVQR 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EW VERD P AT+FVRAYAF+S   ++AYGQTTD KK TNLF++ AI+GRH SLDI S 
Sbjct: 121 FEWLVERDVPQATYFVRAYAFDSNDVQLAYGQTTDPKKSTNLFEIHAISGRHASLDICSV 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAA 207
           CFS FSVLSL  FF   KR+AK +++
Sbjct: 181 CFSVFSVLSLFFFFFLEKRKAKASSS 206

[Close]

BLAST of CmoCh18G001580 vs. TAIR10
Match: AT5G50200.3 (AT5G50200.3 nitrate transmembrane transporters)

HSP 1 Score: 188.7 bits (478), Expect = 3.6e-48
Identity = 99/175 (56.57%), Postives = 126/175 (72.00%), Query Frame = 1


Query: 33  LEVTASPKS---GQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDR 92
           L+VT  P     G VL AG D ++++W L+    +GS  +  +K +KVKLCYAP SQVDR
Sbjct: 37  LDVTTKPSREGPGVVLDAGKDTLNITWTLSS---IGSKREAEFKIIKVKLCYAPPSQVDR 96

Query: 93  AWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDE 152
            WRKT D+L KDKTC  +I+ KPY+   KT+Q   WT+ERD PT T+FVRAYA ++ G E
Sbjct: 97  PWRKTHDELFKDKTCPHKIIAKPYD---KTLQSTTWTLERDIPTGTYFVRAYAVDAIGHE 156

Query: 153 VAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 203
           VAYGQ+TD+ K TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 157 VAYGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of CmoCh18G001580 vs. TAIR10
Match: AT4G24730.1 (AT4G24730.1 Calcineurin-like metallo-phosphoesterase superfamily protein)

HSP 1 Score: 178.3 bits (451), Expect = 4.8e-45
Identity = 96/184 (52.17%), Postives = 129/184 (70.11%), Query Frame = 1


Query: 20  SHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVL-NGAAKVGSDLAYKSVKVKLC 79
           SHG  H     R+LE  A+ +   VL+AG D ++++W L + +AKV +D A+K+++VKLC
Sbjct: 265 SHGVHH-----RSLE--AALECPPVLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLC 324

Query: 80  YAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRA 139
           YAP+SQVDR WRKT++ L KD++C  +IV K Y+   KT Q  +WT+  D PT T+FVRA
Sbjct: 325 YAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAYD---KTPQSLDWTIGLDIPTGTYFVRA 384

Query: 140 YAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGK 199
           Y  +  G EVAYGQ+TDE + TNLF V AI+G H+ LDIAS  FS FSV+SL  FF+  K
Sbjct: 385 YGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEK 438

Query: 200 RQAK 203
           R+AK
Sbjct: 445 RKAK 438

[Close]

BLAST of CmoCh18G001580 vs. NCBI nr
Match: gi|659067354|ref|XP_008439011.1| (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo])

HSP 1 Score: 336.7 bits (862), Expect = 3.0e-89
Identity = 166/207 (80.19%), Postives = 181/207 (87.44%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MA++GF L ALFL CFLGFS G+IHLSS+PR LEVTASPK GQVLKAGVDKI V+WVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
             K GSD +YK++K KLCYAPVSQVDRAWRKTEDDL KDKTCQF IVEKPYNP+NKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EWTVERD PT TFFVRAY FNSAG EVAYGQTTD+KKGTNLFQ+E+ITGRH+SLDIAS 
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAA 208
           CFSAFSV+SL GFF   KR+AKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of CmoCh18G001580 vs. NCBI nr
Match: gi|525507162|ref|NP_001267619.1| (high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus])

HSP 1 Score: 331.3 bits (848), Expect = 1.3e-87
Identity = 164/207 (79.23%), Postives = 179/207 (86.47%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MA++ F L ALFL CFLGFSHG+IH SS+PR LEVTASPK GQVLKAGVDKISV+WVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
             K GSD +YK++K KLCYAPVSQVDRAWRKTEDDL KDKTCQF IVEK YNP+NKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EWTV+RD PT TFFVRAY  NSAG+EVAYGQTTD KKGTNLFQ+E+ITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAA 208
           CFSAFSV+SL GFF   KR+AKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of CmoCh18G001580 vs. NCBI nr
Match: gi|951070145|ref|XP_014490580.1| (PREDICTED: high-affinity nitrate transporter 3.1 [Vigna radiata var. radiata])

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-68
Identity = 126/208 (60.58%), Postives = 165/208 (79.33%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAH   + +L + CF G  +G +H SS+ + L+VTASPK GQVL+AG DKI+V+W LN 
Sbjct: 1   MAAHRLVVVSLLIFCFAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNN 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
               G+D AYK++KVKLCYAP+SQ DRAWRKTED+L++DKTCQ +IV KPY+ SN+TVQ+
Sbjct: 61  TLPTGTDSAYKTIKVKLCYAPISQKDRAWRKTEDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EW +ERD P AT+FVRAYAF+S G+EVAYGQTTD KK +NLF++ +I+GRH SLDI S 
Sbjct: 121 FEWVIERDVPKATYFVRAYAFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAAAAE 209
           CFSAFSV++L  FF   KR+AK +++++
Sbjct: 181 CFSAFSVVALFVFFYIEKRKAKASSSSK 208

[Close]

BLAST of CmoCh18G001580 vs. NCBI nr
Match: gi|593784899|ref|XP_007155490.1| (hypothetical protein PHAVU_003G205900g [Phaseolus vulgaris])

HSP 1 Score: 263.1 bits (671), Expect = 4.2e-67
Identity = 125/205 (60.98%), Postives = 158/205 (77.07%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAAHG  + +L + C  G  +G +H SS+   L+VTASPK GQVL+AG DKI+V+W LN 
Sbjct: 1   MAAHGLVVASLLIFCLAGSCYGKVHFSSLKNTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
               G+D AYK++KVKLCYAP+SQ DRAWRKTE++L++DKTCQ +IV KPY+ SNKTVQ+
Sbjct: 61  TVSTGADSAYKTIKVKLCYAPISQKDRAWRKTEEELSRDKTCQHKIVAKPYDASNKTVQR 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EW +E+D P AT+FVRAYAFNS  +EVA+GQTTD KK +NLF + +I+GRH SLDI S 
Sbjct: 121 FEWVIEKDIPKATYFVRAYAFNSNDEEVAFGQTTDAKKSSNLFDINSISGRHASLDICSI 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKAA 206
           CFSAFSV+SL  FF   KRQ K ++
Sbjct: 181 CFSAFSVVSLFVFFYIEKRQGKASS 205

[Close]

BLAST of CmoCh18G001580 vs. NCBI nr
Match: gi|743905683|ref|XP_011046245.1| (PREDICTED: high-affinity nitrate transporter 3.1-like [Populus euphratica])

HSP 1 Score: 259.6 bits (662), Expect = 4.7e-66
Identity = 128/204 (62.75%), Postives = 156/204 (76.47%), Query Frame = 1


Query: 1   MAAHGFPLTALFLLCFLGFSHGHIHLSSIPRNLEVTASPKSGQVLKAGVDKISVSWVLNG 60
           MAA  F L +L L CFL   +G + LSS+PR LEVTASP SGQVLK G DKI+V+W LN 
Sbjct: 1   MAARAFLLASLVLSCFLLPCYGTVLLSSLPRTLEVTASPTSGQVLKGGEDKITVTWGLNQ 60

Query: 61  AAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQK 120
               G+D  YK++KVKLCYAP+SQVDR WRKT DDL KD+TCQ +IV +PYNP+N T Q 
Sbjct: 61  TVAAGTDSTYKTIKVKLCYAPISQVDRGWRKTVDDLKKDRTCQHKIVARPYNPANSTAQS 120

Query: 121 AEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASG 180
            EWTVERD PTAT+F+RAYA+++   EVAYGQTTD  + TNLFQV+AI+GRH+++D  S 
Sbjct: 121 HEWTVERDVPTATYFIRAYAYDADEKEVAYGQTTDAGRTTNLFQVQAISGRHVTVDTCSI 180

Query: 181 CFSAFSVLSLTGFFIAGKRQAKKA 205
           CFS FSV+SL GFF   KR AK++
Sbjct: 181 CFSVFSVVSLFGFFYNEKRNAKRS 204

The following BLAST results are available for this feature:

BLAST of CmoCh18G001580 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NRT31_ARATH	6.3e-47	56.57	High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana GN=NRT3.1 PE=1 SV=...	[more]
NRT32_ARATH	9.2e-46	48.31	High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana GN=NRT3.2 PE=2 SV=...	[more]
NAR21_ORYSJ	2.3e-41	47.78	High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ...	[more]
NAR22_ORYSJ	5.8e-40	45.71	Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ...	[more]

BLAST of CmoCh18G001580 vs. TrEMBL


Match Name	E-value	Identity	Description
C8CHG8_CUCSA	8.8e-88	79.23	High-affinity nitrate transport system component OS=Cucumis sativus GN=NAR2 PE=2...	[more]
V7CBE9_PHAVU	2.9e-67	60.98	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G205900g PE=4 SV=1	[more]
A0A0L9TQ82_PHAAN	4.3e-66	58.65	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g230400 PE=4 SV=1	[more]
A0A0S3R655_PHAAN	4.3e-66	58.65	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G397600 PE=...	[more]
A0A151QX36_CAJCA	3.6e-65	61.17	Uncharacterized protein OS=Cajanus cajan GN=KK1_044187 PE=4 SV=1	[more]

BLAST of CmoCh18G001580 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G50200.3	3.6e-48	56.57	nitrate transmembrane transporters	[more]
AT4G24730.1	4.8e-45	52.17	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]

BLAST of CmoCh18G001580 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659067354\|ref\|XP_008439011.1\|	3.0e-89	80.19	PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo]	[more]
gi\|525507162\|ref\|NP_001267619.1\|	1.3e-87	79.23	high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus]	[more]
gi\|951070145\|ref\|XP_014490580.1\|	1.3e-68	60.58	PREDICTED: high-affinity nitrate transporter 3.1 [Vigna radiata var. radiata]	[more]
gi\|593784899\|ref\|XP_007155490.1\|	4.2e-67	60.98	hypothetical protein PHAVU_003G205900g [Phaseolus vulgaris]	[more]
gi\|743905683\|ref\|XP_011046245.1\|	4.7e-66	62.75	PREDICTED: high-affinity nitrate transporter 3.1-like [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR016605	Transptr_NO3_Nar2

Vocabulary: **Biological Process**
Term	Definition
GO:0010167	response to nitrate
GO:0015706	nitrate transport

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015706	nitrate transport
biological_process	GO:0010167	response to nitrate
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0015112	nitrate transmembrane transporter activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh18G001580.1	CmoCh18G001580.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016605	High-affinity nitrate transporter	PIR	PIRSF012939	NAR2	coord: 1..205 score: 1.6
IPR016605	High-affinity nitrate transporter	PFAM	PF16974	NAR2	coord: 27..200 score: 5.5
None	No IPR available	PANTHER	PTHR34806	FAMILY NOT NAMED	coord: 1..207 score: 2.0E
None	No IPR available	PANTHER	PTHR34806:SF1	HIGH-AFFINITY NITRATE TRANSPORTER 3.1-RELATED	coord: 1..207 score: 2.0E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh18G001580	Cucsa.098830	Cucumber (Gy14) v1	cgycmoB0206
CmoCh18G001580	CmaCh13G007600	Cucurbita maxima (Rimu)	cmacmoB219
CmoCh18G001580	CmaCh18G001960	Cucurbita maxima (Rimu)	cmacmoB408
CmoCh18G001580	Cla005121	Watermelon (97103) v1	cmowmB360
CmoCh18G001580	Csa1G047430	Cucumber (Chinese Long) v2	cmocuB380
CmoCh18G001580	MELO3C002305	Melon (DHL92) v3.5.1	cmomeB356
CmoCh18G001580	ClCG03G003060	Watermelon (Charleston Gray)	cmowcgB350
CmoCh18G001580	CSPI01G08880	Wild cucumber (PI 183967)	cmocpiB383
CmoCh18G001580	Lsi02G004230	Bottle gourd (USVL1VR-Ls)	cmolsiB356
CmoCh18G001580	Cp4.1LG09g10080	Cucurbita pepo (Zucchini)	cmocpeB377
CmoCh18G001580	Cp4.1LG20g03470	Cucurbita pepo (Zucchini)	cmocpeB405
CmoCh18G001580	MELO3C002305.2	Melon (DHL92) v3.6.1	cmomedB408
CmoCh18G001580	CsaV3_1G008910	Cucumber (Chinese Long) v3	cmocucB0457
CmoCh18G001580	Cla97C03G054140	Watermelon (97103) v2	cmowmbB394
CmoCh18G001580	Bhi08G001354	Wax gourd	cmowgoB0517
CmoCh18G001580	CsGy1G008810	Cucumber (Gy14) v2	cgybcmoB082
CmoCh18G001580	Carg07560	Silver-seed gourd	carcmoB1350
CmoCh18G001580	Carg06757	Silver-seed gourd	carcmoB0673

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmoCh18G001580	CmoCh13G007900	Cucurbita moschata (Rifu)	cmocmoB166

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh18G001580	Watermelon (97103) v2	cmowmbB418
CmoCh18G001580	Cucurbita moschata (Rifu)	cmocmoB065
CmoCh18G001580	Cucurbita moschata (Rifu)	cmocmoB101
CmoCh18G001580	Cucumber (Chinese Long) v2	cmocuB390
CmoCh18G001580	Watermelon (Charleston Gray)	cmowcgB373
CmoCh18G001580	Cucurbita pepo (Zucchini)	cmocpeB410
CmoCh18G001580	Melon (DHL92) v3.6.1	cmomedB414