CmaCh13G007600 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh13G007600
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	High-affinity nitrate transporter 3.2-like protein
Location	Cma_Chr13 : 6608034 .. 6609428 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGTGTCATCATCGTCGTCGTCCTCAAGTCTGCTGAAACATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGTTCTGTTTCTTGGGATTCTCTCATGGACATATCCACTTTTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCGCCTAAACCTGGACAAGGTAAGATTTTTCCGCTCCACCAATTAGATATCTTGTAACATTTCTCTAATGCACGCATTATGTAAACCTTGAGAAAGTCAAAAGGGAAAGTTTAAAAGGAGAATATCTACACTGATGAATTCATTACCAATAATATTAGAGCTAGACACTCAATGATTTGCCAATGAGAAGGCTGGACTACGAATGAGGATGAACACAAGATTGAAGGGGATGGATAGGAGACGGTGTGACAACAAGAACGCTAGATTCCAAAGTGGTGGATTTGGGAGTGGATTGTGTGATCACTGAAGAGAAAAAGTTTTAAAAACTTGAAGGAAAGCATATGATCTGAATCCAAAATTGGAACTTTACCCATTTCTAACAACTGGGTTGTCTTTTTTTAATGCAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGTCAAGTGGACCGGGCCTGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGCGAACAAGACCGTACAAAAGTTCGAGTGGACTGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGAGGAAGTGGGCTACGGGCAAACGAGTGACAACAAAAAAGGCACAAACTTGTTTCAAATCAAAGCGATCACAGGTCGCCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTAGCCGGCAAGAGAAAGACCAAGAAGGCAGAGGCGCCGGGACAGTAAGTTAACAAAGATGGAAACTTGCTCTGTTTCTGAGTTCTTTTTTTGAAGTCTTTCTGCATTTTGGGTTTCTCTTGTACTGTAATTTTCTAGGTGAAAAGATGGAAAATAAAGTGTATTAAATTATGAATGTGTTGATTAATGACAATATTTGAAGATATTGGACTTTAGCTTCAATGGAACACAAAAGGTTGCAACACTCCAACCTTAGAACATGGTGGAGTACAAGTTTACTATTTCTGTCTTTTCATTTTTCTACCACTAAGATATTAGATATTTTGTTTGTTGGTGAAAGTTCTCTCAATGTCATATGACTTTTGAAA

mRNA sequence

GAGTGTCATCATCGTCGTCGTCCTCAAGTCTGCTGAAACATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGTTCTGTTTCTTGGGATTCTCTCATGGACATATCCACTTTTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCGCCTAAACCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGTCAAGTGGACCGGGCCTGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGCGAACAAGACCGTACAAAAGTTCGAGTGGACTGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGAGGAAGTGGGCTACGGGCAAACGAGTGACAACAAAAAAGGCACAAACTTGTTTCAAATCAAAGCGATCACAGGTCGCCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTAGCCGGCAAGAGAAAGACCAAGAAGGCAGAGGCGCCGGGACAGTAAGTTAACAAAGATGGAAACTTGCTCTGTTTCTGAGTTCTTTTTTTGAAGTCTTTCTGCATTTTGGGTTTCTCTTGTACTGTAATTTTCTAGGTGAAAAGATGGAAAATAAAGTGTATTAAATTATGAATGTGTTGATTAATGACAATATTTGAAGATATTGGACTTTAGCTTCAATGGAACACAAAAGGTTGCAACACTCCAACCTTAGAACATGGTGGAGTACAAGTTTACTATTTCTGTCTTTTCATTTTTCTACCACTAAGATATTAGATATTTTGTTTGTTGGTGAAAGTTCTCTCAATGTCATATGACTTTTGAAA

Coding sequence (CDS)

ATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGTTCTGTTTCTTGGGATTCTCTCATGGACATATCCACTTTTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCGCCTAAACCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGTCAAGTGGACCGGGCCTGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGCGAACAAGACCGTACAAAAGTTCGAGTGGACTGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGAGGAAGTGGGCTACGGGCAAACGAGTGACAACAAAAAAGGCACAAACTTGTTTCAAATCAAAGCGATCACAGGTCGCCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTAGCCGGCAAGAGAAAGACCAAGAAGGCAGAGGCGCCGGGACAGTAA

Protein sequence

MEGSINMSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKTKKAEAPGQ

Homology

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BLAST of CmaCh13G007600 vs. Swiss-Prot
Match: NRT31_ARATH (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.0e-48
Identity = 104/209 (49.76%), Postives = 140/209 (66.99%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIH---FSSLPR-SLEVTASPK---PGQVLKAGVDKIS 66
           M+    L A+L +   +   HG      F  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 67  VTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 126
           +TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC   I+ KPY+ 
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPYD- 120

Query: 127 ANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHI 186
             KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ++D+ K TNLF ++AI+GRH 
Sbjct: 121 --KTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 187 SLDIASVCFSAFSVLSLSGFFLAGKRKTK 209
           SLDIAS+CFS FSV++L  FF+  KRK K
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

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BLAST of CmaCh13G007600 vs. Swiss-Prot
Match: NRT32_ARATH (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 1.9e-46
Identity = 99/207 (47.83%), Postives = 136/207 (65.70%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIH---FSSLPRSLEVTASP-KPGQVLKAGVDKISVTW 66
           M+ +  L  +L +F  +  S G      F+ L  S+EVTA P K   VL+AG D +++TW
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 67  VL-NGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPAN 126
            L + + K  +D+++KT++ KLCYAP+SQVDR WRKT++ L KD++C   IV K Y+   
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAYD--- 120

Query: 127 KTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISL 186
           KT Q  +WT+  DIPTGT+FVRAY  +  G EV YGQ++D  + TNLF + AI+G H+ L
Sbjct: 121 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 180

Query: 187 DIASVCFSAFSVLSLSGFFLAGKRKTK 209
           DIAS  FS FSV+SL  FF+  KRK K
Sbjct: 181 DIASTFFSVFSVVSLFVFFVMEKRKAK 204

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BLAST of CmaCh13G007600 vs. Swiss-Prot
Match: NAR21_ORYSJ (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 3.1e-41
Identity = 84/180 (46.67%), Postives = 120/180 (66.67%), Query Frame = 1


Query: 30  IHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVS 89
           +  S LP++L V  SPK G+V+ AG + ++VTW LN +  +G+D+++K+V+ KLCYAP S
Sbjct: 34  VFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPAS 93

Query: 90  QVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNS 149
           + DR WRK  DDL KDK CQF +  +PY        +F++ VARDIPT ++FVRAYA ++
Sbjct: 94  RTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDA 153

Query: 150 AGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKTKK 209
           +G EV YGQ+S +      F +  ITG H SL +A+  FS FS+ +L+ FF+  KRK  K
Sbjct: 154 SGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

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BLAST of CmaCh13G007600 vs. Swiss-Prot
Match: NAR22_ORYSJ (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-39
Identity = 93/206 (45.15%), Postives = 124/206 (60.19%), Query Frame = 1


Query: 14  LAALFLFCFLGFSH-------GHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 73
           L  L L   LG  H            S+L ++L V ASP+ GQVL AG D I+VTW LN 
Sbjct: 13  LPLLLLLVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAGEDAITVTWSLNA 72

Query: 74  TVKS---GSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKT 133
           T  +   G+D+ YK V+  LCYAP SQV R WRK  DDLSKDK CQF I  +PY+ A   
Sbjct: 73  TAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFKIAQQPYDGAG-- 132

Query: 134 VQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDI 193
             KFE+TVARD+PT +++VRAYA +++G  V YG+T+ +      F +  ITG   S+++
Sbjct: 133 --KFEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS----FAVAGITGVTASIEV 192

Query: 194 ASVCFSAFSVLSLSGFFLAGKRKTKK 210
           A+   SAFSV +L+ F +   +K  K
Sbjct: 193 AAGVLSAFSVAALAVFLVLENKKKNK 210

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BLAST of CmaCh13G007600 vs. TrEMBL
Match: C8CHG8_CUCSA (High-affinity nitrate transport system component OS=Cucumis sativus GN=NAR2 PE=2 SV=2)

HSP 1 Score: 353.6 bits (906), Expect = 1.7e-94
Identity = 177/211 (83.89%), Postives = 188/211 (89.10%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++N FLLAALFL CFLGFSHG+IHFSSLPR+LEVTASPKPGQVLKAGVDKISVTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV K YNPANKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           FEWTV RDIPTGTFFVRAY  NSAGEEV YGQT+D KKGTNLFQI++ITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTKK--AEAPGQ 216
           CFSAFSV+SL GFF   KRK KK  A APGQ
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAPGQ 211

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BLAST of CmaCh13G007600 vs. TrEMBL
Match: V7CBE9_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G205900g PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 2.9e-70
Identity = 128/202 (63.37%), Postives = 163/202 (80.69%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+G ++A+L +FC  G  +G +HFSSL  +L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHGLVVASLLIFCLAGSCYGKVHFSSLKNTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           TV +G+DS+YKT++ KLCYAP+SQ DRAWRKTE++LS+DKTCQ  IV KPY+ +NKTVQ+
Sbjct: 61  TVSTGADSAYKTIKVKLCYAPISQKDRAWRKTEEELSRDKTCQHKIVAKPYDASNKTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           FEW + +DIP  T+FVRAYAFNS  EEV +GQT+D KK +NLF I +I+GRH SLDI S+
Sbjct: 121 FEWVIEKDIPKATYFVRAYAFNSNDEEVAFGQTTDAKKSSNLFDINSISGRHASLDICSI 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTK 209
           CFSAFSV+SL  FF   KR+ K
Sbjct: 181 CFSAFSVVSLFVFFYIEKRQGK 202

[Close]

BLAST of CmaCh13G007600 vs. TrEMBL
Match: A0A0S3R655_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G397600 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 5.0e-70
Identity = 127/206 (61.65%), Postives = 165/206 (80.10%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+  ++A+L +FC  G  +G +HFSSL ++L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHSLVVASLLIFCLAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           T+ SG+DS+YKT++ KLCYAP+SQ DRAWRKT D+LS+DKTCQ  IV KPY+ +N+TVQ+
Sbjct: 61  TLPSGTDSAYKTIKVKLCYAPISQKDRAWRKTVDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           FEW + RD+P  T+FVRAY F+S GEEV YGQT+D KK +NLF+I +I+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYVFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTKKAEA 213
           CFSAFSV++L  FF   KRK K + +
Sbjct: 181 CFSAFSVVALFVFFYIEKRKGKASSS 206

[Close]

BLAST of CmaCh13G007600 vs. TrEMBL
Match: A0A0L9TQ82_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g230400 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 5.0e-70
Identity = 127/206 (61.65%), Postives = 165/206 (80.10%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+  ++A+L +FC  G  +G +HFSSL ++L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHSLVVASLLIFCLAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           T+ SG+DS+YKT++ KLCYAP+SQ DRAWRKT D+LS+DKTCQ  IV KPY+ +N+TVQ+
Sbjct: 61  TLPSGTDSAYKTIKVKLCYAPISQKDRAWRKTVDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           FEW + RD+P  T+FVRAY F+S GEEV YGQT+D KK +NLF+I +I+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYVFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTKKAEA 213
           CFSAFSV++L  FF   KRK K + +
Sbjct: 181 CFSAFSVVALFVFFYIEKRKGKASSS 206

[Close]

BLAST of CmaCh13G007600 vs. TrEMBL
Match: A0A067K4F8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11393 PE=4 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 8.5e-70
Identity = 136/205 (66.34%), Postives = 160/205 (78.05%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+  G  LA++ LF F G S+G IHFSSL ++L VTASP PGQVLKAGVD I+VTW LN 
Sbjct: 1   MAPRGLFLASILLFAFFGSSYGVIHFSSLQKTLLVTASPSPGQVLKAGVDNITVTWGLNQ 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           ++ +G+DS+YKT++ KLCYAPVSQ DRAWRKTED+LSKDKTCQ  IV KPY+ ANK    
Sbjct: 61  SLPAGTDSAYKTIKIKLCYAPVSQKDRAWRKTEDNLSKDKTCQVKIVTKPYSSANKN-NS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           F WTV RD+PT T+FVRAYAFN+  +EV YGQT+D  K TN F I+AITGRH SLDIASV
Sbjct: 121 FTWTVERDVPTATYFVRAYAFNANEQEVAYGQTTDVHKTTNFFDIQAITGRHASLDIASV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTKKAE 212
           CFS FSV++L GFF   KRK KK E
Sbjct: 181 CFSVFSVVALFGFFYIEKRKAKKTE 204

[Close]

BLAST of CmaCh13G007600 vs. TAIR10
Match: AT5G50200.3 (AT5G50200.3 nitrate transmembrane transporters)

HSP 1 Score: 193.7 bits (491), Expect = 1.1e-49
Identity = 104/209 (49.76%), Postives = 140/209 (66.99%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIH---FSSLPR-SLEVTASPK---PGQVLKAGVDKIS 66
           M+    L A+L +   +   HG      F  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 67  VTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 126
           +TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC   I+ KPY+ 
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPYD- 120

Query: 127 ANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHI 186
             KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ++D+ K TNLF ++AI+GRH 
Sbjct: 121 --KTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 187 SLDIASVCFSAFSVLSLSGFFLAGKRKTK 209
           SLDIAS+CFS FSV++L  FF+  KRK K
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of CmaCh13G007600 vs. TAIR10
Match: AT4G24730.1 (AT4G24730.1 Calcineurin-like metallo-phosphoesterase superfamily protein)

HSP 1 Score: 178.7 bits (452), Expect = 3.8e-45
Identity = 93/184 (50.54%), Postives = 123/184 (66.85%), Query Frame = 1


Query: 26  SHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVL-NGTVKSGSDSSYKTVEAKLC 85
           SHG  H     RSLE      P  VL+AG D +++TW L + + K  +D+++KT++ KLC
Sbjct: 265 SHGVHH-----RSLEAALECPP--VLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLC 324

Query: 86  YAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRA 145
           YAP+SQVDR WRKT++ L KD++C   IV K Y+   KT Q  +WT+  DIPTGT+FVRA
Sbjct: 325 YAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAYD---KTPQSLDWTIGLDIPTGTYFVRA 384

Query: 146 YAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASVCFSAFSVLSLSGFFLAGK 205
           Y  +  G EV YGQ++D  + TNLF + AI+G H+ LDIAS  FS FSV+SL  FF+  K
Sbjct: 385 YGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEK 438

Query: 206 RKTK 209
           RK K
Sbjct: 445 RKAK 438

[Close]

BLAST of CmaCh13G007600 vs. NCBI nr
Match: gi|525507162|ref|NP_001267619.1| (high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus])

HSP 1 Score: 353.6 bits (906), Expect = 2.4e-94
Identity = 177/211 (83.89%), Postives = 188/211 (89.10%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++N FLLAALFL CFLGFSHG+IHFSSLPR+LEVTASPKPGQVLKAGVDKISVTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV K YNPANKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           FEWTV RDIPTGTFFVRAY  NSAGEEV YGQT+D KKGTNLFQI++ITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTKK--AEAPGQ 216
           CFSAFSV+SL GFF   KRK KK  A APGQ
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAPGQ 211

[Close]

BLAST of CmaCh13G007600 vs. NCBI nr
Match: gi|659067354|ref|XP_008439011.1| (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo])

HSP 1 Score: 352.8 bits (904), Expect = 4.2e-94
Identity = 172/209 (82.30%), Postives = 188/209 (89.95%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++NGFLLAALFL CFLGFS G+IH SSLPR+LEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV KPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           FEWTV RD+PTGTFFVRAY FNSAG+EV YGQT+D+KKGTNLFQI++ITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTKKAEAPGQ 216
           CFSAFSV+SL GFF   KRK KKA A  +
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAE 209

[Close]

BLAST of CmaCh13G007600 vs. NCBI nr
Match: gi|951070145|ref|XP_014490580.1| (PREDICTED: high-affinity nitrate transporter 3.1 [Vigna radiata var. radiata])

HSP 1 Score: 276.9 bits (707), Expect = 2.9e-71
Identity = 128/206 (62.14%), Postives = 167/206 (81.07%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+  ++ +L +FCF G  +G +HFSSL ++L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHRLVVVSLLIFCFAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNN 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           T+ +G+DS+YKT++ KLCYAP+SQ DRAWRKTED+LS+DKTCQ  IV KPY+ +N+TVQ+
Sbjct: 61  TLPTGTDSAYKTIKVKLCYAPISQKDRAWRKTEDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           FEW + RD+P  T+FVRAYAF+S GEEV YGQT+D KK +NLF+I +I+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYAFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTKKAEA 213
           CFSAFSV++L  FF   KRK K + +
Sbjct: 181 CFSAFSVVALFVFFYIEKRKAKASSS 206

[Close]

BLAST of CmaCh13G007600 vs. NCBI nr
Match: gi|593784899|ref|XP_007155490.1| (hypothetical protein PHAVU_003G205900g [Phaseolus vulgaris])

HSP 1 Score: 273.1 bits (697), Expect = 4.2e-70
Identity = 128/202 (63.37%), Postives = 163/202 (80.69%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+G ++A+L +FC  G  +G +HFSSL  +L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHGLVVASLLIFCLAGSCYGKVHFSSLKNTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           TV +G+DS+YKT++ KLCYAP+SQ DRAWRKTE++LS+DKTCQ  IV KPY+ +NKTVQ+
Sbjct: 61  TVSTGADSAYKTIKVKLCYAPISQKDRAWRKTEEELSRDKTCQHKIVAKPYDASNKTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           FEW + +DIP  T+FVRAYAFNS  EEV +GQT+D KK +NLF I +I+GRH SLDI S+
Sbjct: 121 FEWVIEKDIPKATYFVRAYAFNSNDEEVAFGQTTDAKKSSNLFDINSISGRHASLDICSI 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTK 209
           CFSAFSV+SL  FF   KR+ K
Sbjct: 181 CFSAFSVVSLFVFFYIEKRQGK 202

[Close]

BLAST of CmaCh13G007600 vs. NCBI nr
Match: gi|920688765|gb|KOM32748.1| (hypothetical protein LR48_Vigan01g230400 [Vigna angularis])

HSP 1 Score: 272.3 bits (695), Expect = 7.1e-70
Identity = 127/206 (61.65%), Postives = 165/206 (80.10%), Query Frame = 1


Query: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M+A+  ++A+L +FC  G  +G +HFSSL ++L+VTASPK GQVL+AG DKI+VTW LN 
Sbjct: 1   MAAHSLVVASLLIFCLAGSCYGKVHFSSLKKTLDVTASPKQGQVLEAGTDKITVTWALNK 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126
           T+ SG+DS+YKT++ KLCYAP+SQ DRAWRKT D+LS+DKTCQ  IV KPY+ +N+TVQ+
Sbjct: 61  TLPSGTDSAYKTIKVKLCYAPISQKDRAWRKTVDELSRDKTCQHKIVAKPYDASNRTVQR 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186
           FEW + RD+P  T+FVRAY F+S GEEV YGQT+D KK +NLF+I +I+GRH SLDI SV
Sbjct: 121 FEWVIERDVPKATYFVRAYVFDSNGEEVAYGQTTDAKKSSNLFEINSISGRHASLDICSV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKTKKAEA 213
           CFSAFSV++L  FF   KRK K + +
Sbjct: 181 CFSAFSVVALFVFFYIEKRKGKASSS 206

The following BLAST results are available for this feature:

BLAST of CmaCh13G007600 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NRT31_ARATH	2.0e-48	49.76	High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana GN=NRT3.1 PE=1 SV=...	[more]
NRT32_ARATH	1.9e-46	47.83	High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana GN=NRT3.2 PE=2 SV=...	[more]
NAR21_ORYSJ	3.1e-41	46.67	High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ...	[more]
NAR22_ORYSJ	1.3e-39	45.15	Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ...	[more]

BLAST of CmaCh13G007600 vs. TrEMBL


Match Name	E-value	Identity	Description
C8CHG8_CUCSA	1.7e-94	83.89	High-affinity nitrate transport system component OS=Cucumis sativus GN=NAR2 PE=2...	[more]
V7CBE9_PHAVU	2.9e-70	63.37	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G205900g PE=4 SV=1	[more]
A0A0S3R655_PHAAN	5.0e-70	61.65	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G397600 PE=...	[more]
A0A0L9TQ82_PHAAN	5.0e-70	61.65	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g230400 PE=4 SV=1	[more]
A0A067K4F8_JATCU	8.5e-70	66.34	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11393 PE=4 SV=1	[more]

BLAST of CmaCh13G007600 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G50200.3	1.1e-49	49.76	nitrate transmembrane transporters	[more]
AT4G24730.1	3.8e-45	50.54	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]

BLAST of CmaCh13G007600 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|525507162\|ref\|NP_001267619.1\|	2.4e-94	83.89	high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus]	[more]
gi\|659067354\|ref\|XP_008439011.1\|	4.2e-94	82.30	PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo]	[more]
gi\|951070145\|ref\|XP_014490580.1\|	2.9e-71	62.14	PREDICTED: high-affinity nitrate transporter 3.1 [Vigna radiata var. radiata]	[more]
gi\|593784899\|ref\|XP_007155490.1\|	4.2e-70	63.37	hypothetical protein PHAVU_003G205900g [Phaseolus vulgaris]	[more]
gi\|920688765\|gb\|KOM32748.1\|	7.1e-70	61.65	hypothetical protein LR48_Vigan01g230400 [Vigna angularis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR016605	Transptr_NO3_Nar2

Vocabulary: **Biological Process**
Term	Definition
GO:0010167	response to nitrate
GO:0015706	nitrate transport

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015706	nitrate transport
biological_process	GO:0010167	response to nitrate
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0015112	nitrate transmembrane transporter activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh13G007600.1	CmaCh13G007600.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016605	High-affinity nitrate transporter	PIR	PIRSF012939	NAR2	coord: 7..211 score: 1.0
IPR016605	High-affinity nitrate transporter	PFAM	PF16974	NAR2	coord: 32..206 score: 1.4
None	No IPR available	PANTHER	PTHR34806	FAMILY NOT NAMED	coord: 4..213 score: 2.0E
None	No IPR available	PANTHER	PTHR34806:SF1	HIGH-AFFINITY NITRATE TRANSPORTER 3.1-RELATED	coord: 4..213 score: 2.0E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh13G007600	Cla005121	Watermelon (97103) v1	cmawmB187
CmaCh13G007600	Csa1G047430	Cucumber (Chinese Long) v2	cmacuB204
CmaCh13G007600	MELO3C002305	Melon (DHL92) v3.5.1	cmameB197
CmaCh13G007600	ClCG03G003060	Watermelon (Charleston Gray)	cmawcgB196
CmaCh13G007600	CSPI01G08880	Wild cucumber (PI 183967)	cmacpiB206
CmaCh13G007600	CmoCh13G007900	Cucurbita moschata (Rifu)	cmacmoB218
CmaCh13G007600	CmoCh18G001580	Cucurbita moschata (Rifu)	cmacmoB219
CmaCh13G007600	Lsi02G004230	Bottle gourd (USVL1VR-Ls)	cmalsiB202
CmaCh13G007600	Cp4.1LG09g10080	Cucurbita pepo (Zucchini)	cmacpeB214
CmaCh13G007600	Cp4.1LG20g03470	Cucurbita pepo (Zucchini)	cmacpeB234
CmaCh13G007600	MELO3C002305.2	Melon (DHL92) v3.6.1	cmamedB218
CmaCh13G007600	CsaV3_1G008910	Cucumber (Chinese Long) v3	cmacucB0235
CmaCh13G007600	Cla97C03G054140	Watermelon (97103) v2	cmawmbB227
CmaCh13G007600	Bhi08G001354	Wax gourd	cmawgoB0258
CmaCh13G007600	CsGy1G008810	Cucumber (Gy14) v2	cgybcmaB063
CmaCh13G007600	Carg07560	Silver-seed gourd	carcmaB1393
CmaCh13G007600	Carg06757	Silver-seed gourd	carcmaB0689

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh13G007600	CmaCh18G001960	Cucurbita maxima (Rimu)	cmacmaB210

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh13G007600	Cucumber (Gy14) v1	cgycmaB0162