Lsi10G010990 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G010990
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Pre-mRNA branch site p14-like protein
Location	chr10 : 15257728 .. 15260175 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCACAAATCACCCCCTCCGAAATTTCCCGCATTTCGAATTTTCTCGCACTTTCTCTTCTCCCAAACAGGCTCACAAGAAACCTTCCTCCATGGCCACATTCAGCCTTCCCCGCAAGGGAAATACGAGGCTGCCGCCGGAGGTTAACAGAGTCTTGTTTGTAAGAAATTTGCCCTTTAACATTACCAGCGAAGAGATGTACGATATCTTCGGCAAGTACGGGGCGATACGGCAGATTCGGATTGGGACGAACAAGGATACTAGAGGTACCGCCTTTGTTGTTTACGAAGATATCTATGATGCTAAGACGGCGGTGGATCACCTCTCCGGTTTCAACGTTGCCAATCGGTATCTGATCGTGTTGTACTACCAGCAAGCCAAGATGAGCAAAAAGTTCGACCAGAAGAAGAAAGAGGATGAACTCGCTAGGATGCAGGAGAAGTACGGTGTTTCCACGAAAGATAAGTAAAAGGTGTAATATTAAACTTATTTTTCTTAGGATTTTCTTAAGCAATCCTGGAATTGAGTCGAATTGTTTATAACTTGAAGTTATCTCTTTCTAGGGTTTCGTAGCTGTACTAATTTTCTTAAATTGACAGTGTTTTTCTTATACTCTATATGCTCTGATCATGGCACGAGTCTGTTTGAGTAATTTATTTTGTTTAATTATAATTAAATTGTAAGCATTCATTCTTAACGCCTACCCGGCTTTTTTCATTTCTCTTATATGGTTGGAGAACTTTGGTAGTTAACTTCTAAGATATGAATGTGAAAGTTATGACTTTGTGAGTGTGTGTAGTAAGCTTGGTGTGGTGGCTTTTATCTAATTGAAATAATCGCGCAATAGTTTTTTCAGGCTTAAGCGCAATATGAGTTTGCACTATATATAAAAGTATTATATAAAGAAGAGGGAGCATCGTTGAAGTGGAAATATGAAGGAATAAAAAATTCTAAACCCAAGGAATTTTGCATTTAGACTTAGGAAATGTATTTCTCTTAGTTAATAAGGAAGGAAAAACCCTGATTTTTTAAAAATAATAAAAAAACCTCAAGGCCTAGGGCTTGAACCTTGGAGCTTCATAAAAGGCGTGTGCCTAATGCCCGCCTTATTTTGTGATTCTCGCCTTGCCAGACCTACGCCTAGTCGCTAGGCCATGCCTAACATCTTTTTGAAAATCTGTTTGCACTTTGCATGACTAGGATCTATTTGGGTTTCTCAAAAGGTTCTTGTTGGGAGAGTAAAACGGAGGAAGTTGTGGCAAATAAGAAACCAACCTATGAGTTAGACTGTACGAATCTATTATAACTCTATTTTCTTTATTGGTTGTTCTATAGTAACTTTCTATGATGGAGACTTTTTAATTTGCAATATTCATATGCTTATAAGATGTATTATTGCCTATGTTGGCTGATTTTCTTGTACCCTTTCCCCCTATTTGTATTCTGCGTGTGCTTTGGCTCTCTTAGGCTTAGACCTTCTATTCTGCTGTGCCATCTGAATAGCCTTGCACGGCATGGAGTATCGTAAATTATTCGAACGATCTTTTATTTTGAAGGCATGCTGTGAGAAAGATTTCCTTAATTTTAATGCTCTACATAGGAAGTCATTCTGAACAGGTACACCATGTATAGAAATAAGAGTGAAAATGAGAAGATACCTATCTATTACTTATGTTGACATTATCTTCTATATCAATCTAAGTTATACCTCTTCACATTCAGCATGTTCTTGTGGAAAGTTATCAAAGTTTTCAAGGATACTAAGTACGACTGTAAGTTAGGAATGTATTGGGATAATTAGTATGATTAGTAGTAAGGAATTTTGTAGTAATTAAACATTAAGTTGGTTAGGTATTTTAGTTATAAATAGAGATAGTGGGTTGAGGGAAAGGGTGTGAAGAATTTTATGTGGGATTTCCTAATTGAGAATTTGGGATGGATTCTCAACTCTCTAGAATGTGCTTGAGGTATATTGTAATTTCTTTATTGATATTGCAATATAATTCTATCTTTTAGTTCTTTTGTTTGTTCTTTGTGTTCTTGAATTCTCTTGTTAAGAGGTATCTTAACAAAGGTCTTTTCGTTTGCGCTGTTGTTAGGAATTCCCGTGGAGCGTTTGGAAAGAAAAGGAAAAGTATCTTTGAGGAGGATGATTCAGAAAGAAGGCATCTTGTTGAATGTTGGCCCTTACCTAAAGTTCTCCCTTTCATGTTTGTTGTATTACAATTTCGTGGACGTTACTTTTTATATTTGATGATTGGATTTCATTGTGTCCAACAAGTATGAAAGAAGATCCTCGAGCATAGTTCAGTAAAAAAGCTTATTTTGTTCTGCTTAAATACCATACTTTCCATCCAATGGAATTTCTCCATACAGATCTTTTTGTATATGACGTGTGCTCATTGAGTTTGATTTGCAAATTCACAAATTCTAAGATCGATTGAAAGCA

mRNA sequence

GGCACAAATCACCCCCTCCGAAATTTCCCGCATTTCGAATTTTCTCGCACTTTCTCTTCTCCCAAACAGGCTCACAAGAAACCTTCCTCCATGGCCACATTCAGCCTTCCCCGCAAGGGAAATACGAGGCTGCCGCCGGAGGTTAACAGAGTCTTGTTTGTAAGAAATTTGCCCTTTAACATTACCAGCGAAGAGATGTACGATATCTTCGGCAAGTACGGGGCGATACGGCAGATTCGGATTGGGACGAACAAGGATACTAGAGGTACCGCCTTTGTTGTTTACGAAGATATCTATGATGCTAAGACGGCGGTGGATCACCTCTCCGGTTTCAACGTTGCCAATCGGTATCTGATCGTGTTGTACTACCAGCAAGCCAAGATGAGCAAAAAGTTCGACCAGAAGAAGAAAGAGGATGAACTCGCTAGGATGCAGGAGAAGTACGGTGTTTCCACGAAAGATAAGTAAGAATTCCCGTGGAGCGTTTGGAAAGAAAAGGAAAAGTATCTTTGAGGAGGATGATTCAGAAAGAAGGCATCTTGTTGAATGTTGGCCCTTACCTAAAGTTCTCCCTTTCATGTTTGTTGTATTACAATTTCGTGGACGTTACTTTTTATATTTGATGATTGGATTTCATTGTGTCCAACAAGTATGAAAGAAGATCCTCGAGCATAGTTCAGTAAAAAAGCTTATTTTGTTCTGCTTAAATACCATACTTTCCATCCAATGGAATTTCTCCATACAGATCTTTTTGTATATGACGTGTGCTCATTGAGTTTGATTTGCAAATTCACAAATTCTAAGATCGATTGAAAGCA

Coding sequence (CDS)

ATGGCCACATTCAGCCTTCCCCGCAAGGGAAATACGAGGCTGCCGCCGGAGGTTAACAGAGTCTTGTTTGTAAGAAATTTGCCCTTTAACATTACCAGCGAAGAGATGTACGATATCTTCGGCAAGTACGGGGCGATACGGCAGATTCGGATTGGGACGAACAAGGATACTAGAGGTACCGCCTTTGTTGTTTACGAAGATATCTATGATGCTAAGACGGCGGTGGATCACCTCTCCGGTTTCAACGTTGCCAATCGGTATCTGATCGTGTTGTACTACCAGCAAGCCAAGATGAGCAAAAAGTTCGACCAGAAGAAGAAAGAGGATGAACTCGCTAGGATGCAGGAGAAGTACGGTGTTTCCACGAAAGATAAGTAA

Protein sequence

MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGVSTKDK

Homology

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BLAST of Lsi10G010990 vs. Swiss-Prot
Match: SF3B6_ARATH (Splicing factor 3B subunit 6-like protein OS=Arabidopsis thaliana GN=At5g12190 PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 9.9e-56
Identity = 109/125 (87.20%), Postives = 115/125 (92.00%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           M T SL RK NTRLPPEVNRVL+VRNLPFNITSEEMYDIFGKYGAIRQIRIG +K T+GT
Sbjct: 1   MTTISL-RKSNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGCDKATKGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAK AVDHLSGFNVANRYLIVLYYQ AKMSKKFDQKK EDE+ ++QEKYGV
Sbjct: 61  AFVVYEDIYDAKNAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKSEDEITKLQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

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BLAST of Lsi10G010990 vs. Swiss-Prot
Match: SF3B6_HUMAN (Splicing factor 3B subunit 6 OS=Homo sapiens GN=SF3B6 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 1.8e-41
Identity = 76/115 (66.09%), Postives = 96/115 (83.48%), Query Frame = 1


Query: 8   RKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVVYED 67
           ++ N RLPPEVNR+L++RNLP+ IT+EEMYDIFGKYG IRQIR+G   +TRGTA+VVYED
Sbjct: 7   KRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYED 66

Query: 68  IYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGVST 123
           I+DAK A DHLSGFNV NRYL+VLYY   +  +K D KKKE++L  ++EKYG++T
Sbjct: 67  IFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINT 121

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BLAST of Lsi10G010990 vs. Swiss-Prot
Match: SF3B6_MOUSE (Splicing factor 3B subunit 6 OS=Mus musculus GN=Sf3b6 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 1.8e-41
Identity = 76/115 (66.09%), Postives = 96/115 (83.48%), Query Frame = 1


Query: 8   RKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVVYED 67
           ++ N RLPPEVNR+L++RNLP+ IT+EEMYDIFGKYG IRQIR+G   +TRGTA+VVYED
Sbjct: 7   KRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYED 66

Query: 68  IYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGVST 123
           I+DAK A DHLSGFNV NRYL+VLYY   +  +K D KKKE++L  ++EKYG++T
Sbjct: 67  IFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINT 121

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BLAST of Lsi10G010990 vs. Swiss-Prot
Match: SF3B6_DROME (Splicing factor 3B subunit 6-like protein OS=Drosophila melanogaster GN=CG13298 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 7.6e-40
Identity = 76/117 (64.96%), Postives = 95/117 (81.20%), Query Frame = 1


Query: 8   RKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVVYED 67
           ++ + RLPPEVNR+L+VRNLP+ ITS+EMYDIFGK+GAIRQIR+G   +TRGTAFVVYED
Sbjct: 3   KRNHIRLPPEVNRLLYVRNLPYKITSDEMYDIFGKFGAIRQIRVGNTPETRGTAFVVYED 62

Query: 68  IYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGVSTKD 125
           I+DAK A DHLSGFNV NRYL+VLYYQ  K  K+ D  KK++EL  ++ KY + T +
Sbjct: 63  IFDAKNACDHLSGFNVCNRYLVVLYYQSNKAFKRVDMDKKQEELNNIKAKYNLKTPE 119

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BLAST of Lsi10G010990 vs. Swiss-Prot
Match: SF3B6_CAEEL (Splicing factor 3B subunit 6-like protein OS=Caenorhabditis elegans GN=C50D2.5 PE=3 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 4.6e-37
Identity = 68/108 (62.96%), Postives = 92/108 (85.19%), Query Frame = 1


Query: 13  RLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVVYEDIYDAK 72
           +LPPEVNR+L+++NLP+ IT+EEMY+IFGK+GA+RQIR+G   +TRGTAFVVYEDI+DAK
Sbjct: 12  KLPPEVNRILYIKNLPYKITTEEMYEIFGKFGAVRQIRVGNTAETRGTAFVVYEDIFDAK 71

Query: 73  TAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 121
           TA +HLSG+NV+NRYL+VLYYQ  K  K+ D +K   +L  ++E+YG+
Sbjct: 72  TACEHLSGYNVSNRYLVVLYYQATKAWKRMDTEKARTKLEDIKERYGI 119

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BLAST of Lsi10G010990 vs. TrEMBL
Match: A0A0A0KI93_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G430680 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 9.0e-64
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV
Sbjct: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 125

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BLAST of Lsi10G010990 vs. TrEMBL
Match: B7FGI3_MEDTR (Pre-mRNA branch site p14-like protein OS=Medicago truncatula GN=MTR_5g089300 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 7.9e-60
Identity = 120/125 (96.00%), Postives = 122/125 (97.60%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           M T SL RKGNTRLPPEVNRVL+VRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MTTISL-RKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDE+ARMQEKYGV
Sbjct: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

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BLAST of Lsi10G010990 vs. TrEMBL
Match: V7B6G4_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_008G202000g PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.0e-59
Identity = 120/125 (96.00%), Postives = 122/125 (97.60%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           MA  SL RKGNTRLPPEVNRVL+VRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MAAISL-RKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDE+ARMQEKYGV
Sbjct: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

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BLAST of Lsi10G010990 vs. TrEMBL
Match: A0A0S3RK02_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.03G048500 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.0e-59
Identity = 120/125 (96.00%), Postives = 122/125 (97.60%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           MA  SL RKGNTRLPPEVNRVL+VRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MAAISL-RKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDE+ARMQEKYGV
Sbjct: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

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BLAST of Lsi10G010990 vs. TrEMBL
Match: A0A0L9UUV9_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan07g035900 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.0e-59
Identity = 120/125 (96.00%), Postives = 122/125 (97.60%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           MA  SL RKGNTRLPPEVNRVL+VRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MAAISL-RKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDE+ARMQEKYGV
Sbjct: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

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BLAST of Lsi10G010990 vs. TAIR10
Match: AT5G12190.1 (AT5G12190.1 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 217.2 bits (552), Expect = 5.6e-57
Identity = 109/125 (87.20%), Postives = 115/125 (92.00%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           M T SL RK NTRLPPEVNRVL+VRNLPFNITSEEMYDIFGKYGAIRQIRIG +K T+GT
Sbjct: 1   MTTISL-RKSNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGCDKATKGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAK AVDHLSGFNVANRYLIVLYYQ AKMSKKFDQKK EDE+ ++QEKYGV
Sbjct: 61  AFVVYEDIYDAKNAVDHLSGFNVANRYLIVLYYQHAKMSKKFDQKKSEDEITKLQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

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BLAST of Lsi10G010990 vs. TAIR10
Match: AT2G14870.1 (AT2G14870.1 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 111.7 bits (278), Expect = 3.3e-25
Identity = 50/70 (71.43%), Postives = 59/70 (84.29%), Query Frame = 1


Query: 9  KGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVVYEDI 68
          K N RLPPEV R+L++ NLPF+ITSE+ YD+FG+Y  IRQ+RIG  K T+GTAFVVYEDI
Sbjct: 9  KQNPRLPPEVTRLLYICNLPFSITSEDTYDLFGRYSTIRQVRIGCEKGTKGTAFVVYEDI 68

Query: 69 YDAKTAVDHL 79
          YDAK AVDHL
Sbjct: 69 YDAKKAVDHL 78

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BLAST of Lsi10G010990 vs. TAIR10
Match: AT2G30260.1 (AT2G30260.1 U2 small nuclear ribonucleoprotein B)

HSP 1 Score: 51.6 bits (122), Expect = 4.1e-07
Identity = 30/89 (33.71%), Postives = 46/89 (51.69%), Query Frame = 1


Query: 5   SLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVV 64
           S    G   +PP  N +LF++NLP   TS  +  +F +Y   ++IR+   K   G AFV 
Sbjct: 145 SFQPSGQETMPP--NNILFIQNLPHETTSMMLQLLFEQYPGFKEIRMIDAKP--GIAFVE 204

Query: 65  YEDIYDAKTAVDHLSGFNVANRYLIVLYY 94
           YED   A  A+  L GF +  +  +V+ +
Sbjct: 205 YEDDVQASIAMQPLQGFKITPQNPMVISF 229

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BLAST of Lsi10G010990 vs. TAIR10
Match: AT4G02430.2 (AT4G02430.2 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 50.4 bits (119), Expect = 9.0e-07
Identity = 26/72 (36.11%), Postives = 39/72 (54.17%), Query Frame = 1


Query: 19 NRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVVYEDIYDAKTAVDHL 78
          +R ++V NLP +I   E+ D+F KYG + QI +       G AFV +ED  DA  A+   
Sbjct: 6  SRTIYVGNLPGDIREREVEDLFSKYGPVVQIDLKIPPRPPGYAFVEFEDARDADDAIYGR 65

Query: 79 SGFNVANRYLIV 91
           G++    +L V
Sbjct: 66 DGYDFDGHHLRV 77

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BLAST of Lsi10G010990 vs. TAIR10
Match: AT5G07290.1 (AT5G07290.1 MEI2-like 4)

HSP 1 Score: 50.4 bits (119), Expect = 9.0e-07
Identity = 27/85 (31.76%), Postives = 50/85 (58.82%), Query Frame = 1


Query: 4   FSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFV 63
           +S+P++       E    L+V NL  +I++EE++ IF  YG IR++R   +++++   ++
Sbjct: 281 YSIPKENPKENSSE--GALWVNNLDSSISNEELHGIFSSYGEIREVRRTMHENSQ--VYI 340

Query: 64  VYEDIYDAKTAVDHLSGFNVANRYL 89
            + D+  AK A+  L+G  VA R L
Sbjct: 341 EFFDVRKAKVALQGLNGLEVAGRQL 361

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BLAST of Lsi10G010990 vs. NCBI nr
Match: gi|659097475|ref|XP_008449647.1| (PREDICTED: splicing factor 3B subunit 6-like protein [Cucumis melo])

HSP 1 Score: 250.8 bits (639), Expect = 1.3e-63
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV
Sbjct: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 125

[Close]

BLAST of Lsi10G010990 vs. NCBI nr
Match: gi|922357372|ref|XP_003617230.2| (Pre-mRNA branch site p14-like protein [Medicago truncatula])

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-59
Identity = 120/125 (96.00%), Postives = 122/125 (97.60%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           M T SL RKGNTRLPPEVNRVL+VRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MTTISL-RKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDE+ARMQEKYGV
Sbjct: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

[Close]

BLAST of Lsi10G010990 vs. NCBI nr
Match: gi|356501392|ref|XP_003519509.1| (PREDICTED: splicing factor 3B subunit 6-like protein [Glycine max])

HSP 1 Score: 237.3 bits (604), Expect = 1.5e-59
Identity = 120/125 (96.00%), Postives = 122/125 (97.60%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           MA  SL RKGNTRLPPEVNRVL+VRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MAAISL-RKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDE+ARMQEKYGV
Sbjct: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

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BLAST of Lsi10G010990 vs. NCBI nr
Match: gi|356554173|ref|XP_003545423.1| (PREDICTED: splicing factor 3B subunit 6-like protein [Glycine max])

HSP 1 Score: 236.9 bits (603), Expect = 1.9e-59
Identity = 119/125 (95.20%), Postives = 122/125 (97.60%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           MA  SL RKGNTRLPPEVNRVL+VRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MAAISL-RKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AF+VYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDE+ARMQEKYGV
Sbjct: 61  AFIVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

[Close]

BLAST of Lsi10G010990 vs. NCBI nr
Match: gi|802558799|ref|XP_012065832.1| (PREDICTED: splicing factor 3B subunit 6-like protein [Jatropha curcas])

HSP 1 Score: 236.1 bits (601), Expect = 3.3e-59
Identity = 119/125 (95.20%), Postives = 123/125 (98.40%), Query Frame = 1


Query: 1   MATFSLPRKGNTRLPPEVNRVLFVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60
           MAT SL RKGNTRLPPEVNRVL+VRNLPFNI+SEEMYDIFGKYGAIRQIRIGTNKDTRGT
Sbjct: 1   MATISL-RKGNTRLPPEVNRVLYVRNLPFNISSEEMYDIFGKYGAIRQIRIGTNKDTRGT 60

Query: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDELARMQEKYGV 120
           AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKE+E+ARMQEKYGV
Sbjct: 61  AFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEEEIARMQEKYGV 120

Query: 121 STKDK 126
           STKDK
Sbjct: 121 STKDK 124

The following BLAST results are available for this feature:

BLAST of Lsi10G010990 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SF3B6_ARATH	9.9e-56	87.20	Splicing factor 3B subunit 6-like protein OS=Arabidopsis thaliana GN=At5g12190 P...	[more]
SF3B6_HUMAN	1.8e-41	66.09	Splicing factor 3B subunit 6 OS=Homo sapiens GN=SF3B6 PE=1 SV=1	[more]
SF3B6_MOUSE	1.8e-41	66.09	Splicing factor 3B subunit 6 OS=Mus musculus GN=Sf3b6 PE=1 SV=1	[more]
SF3B6_DROME	7.6e-40	64.96	Splicing factor 3B subunit 6-like protein OS=Drosophila melanogaster GN=CG13298 ...	[more]
SF3B6_CAEEL	4.6e-37	62.96	Splicing factor 3B subunit 6-like protein OS=Caenorhabditis elegans GN=C50D2.5 P...	[more]

BLAST of Lsi10G010990 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KI93_CUCSA	9.0e-64	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G430680 PE=4 SV=1	[more]
B7FGI3_MEDTR	7.9e-60	96.00	Pre-mRNA branch site p14-like protein OS=Medicago truncatula GN=MTR_5g089300 PE=...	[more]
V7B6G4_PHAVU	1.0e-59	96.00	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_008G202000g PE=4 SV=1	[more]
A0A0S3RK02_PHAAN	1.0e-59	96.00	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.03G048500 PE=...	[more]
A0A0L9UUV9_PHAAN	1.0e-59	96.00	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan07g035900 PE=4 SV=1	[more]

BLAST of Lsi10G010990 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G12190.1	5.6e-57	87.20	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT2G14870.1	3.3e-25	71.43	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT2G30260.1	4.1e-07	33.71	U2 small nuclear ribonucleoprotein B	[more]
AT4G02430.2	9.0e-07	36.11	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT5G07290.1	9.0e-07	31.76	MEI2-like 4	[more]

BLAST of Lsi10G010990 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659097475\|ref\|XP_008449647.1\|	1.3e-63	100.00	PREDICTED: splicing factor 3B subunit 6-like protein [Cucumis melo]	[more]
gi\|922357372\|ref\|XP_003617230.2\|	1.1e-59	96.00	Pre-mRNA branch site p14-like protein [Medicago truncatula]	[more]
gi\|356501392\|ref\|XP_003519509.1\|	1.5e-59	96.00	PREDICTED: splicing factor 3B subunit 6-like protein [Glycine max]	[more]
gi\|356554173\|ref\|XP_003545423.1\|	1.9e-59	95.20	PREDICTED: splicing factor 3B subunit 6-like protein [Glycine max]	[more]
gi\|802558799\|ref\|XP_012065832.1\|	3.3e-59	95.20	PREDICTED: splicing factor 3B subunit 6-like protein [Jatropha curcas]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding

Vocabulary: **INTERPRO**
Term	Definition
IPR012677	Nucleotide-bd_a/b_plait_sf
IPR000504	RRM_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0000398	mRNA splicing, via spliceosome
cellular_component	GO:0071013	catalytic step 2 spliceosome
cellular_component	GO:0071011	precatalytic spliceosome
cellular_component	GO:0005689	U12-type spliceosomal complex
cellular_component	GO:0005686	U2 snRNP
cellular_component	GO:0005684	U2-type spliceosomal complex
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0003723	RNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G010990.1	Lsi10G010990.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 22..88 score: 4.5
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 21..91 score: 1.4
IPR000504	RNA recognition motif domain	PROFILE	PS50102	RRM	coord: 20..95 score: 16
IPR012677	Nucleotide-binding alpha-beta plait domain	GENE3D	G3DSA:3.30.70.330		coord: 19..108 score: 1.5
IPR012677	Nucleotide-binding alpha-beta plait domain	unknown	SSF54928	RNA-binding domain, RBD	coord: 10..103 score: 1.61
None	No IPR available	PANTHER	PTHR12785	FAMILY NOT NAMED	coord: 8..123 score: 4.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi10G010990	Csa6G430680	Cucumber (Chinese Long) v2	culsiB414
Lsi10G010990	CSPI06G23790	Wild cucumber (PI 183967)	cpilsiB417
Lsi10G010990	Cucsa.086930	Cucumber (Gy14) v1	cgylsiB095
Lsi10G010990	CmaCh20G001170	Cucurbita maxima (Rimu)	cmalsiB500
Lsi10G010990	CmaCh02G012820	Cucurbita maxima (Rimu)	cmalsiB540
Lsi10G010990	CmoCh02G013240	Cucurbita moschata (Rifu)	cmolsiB525
Lsi10G010990	CmoCh20G001250	Cucurbita moschata (Rifu)	cmolsiB484
Lsi10G010990	Cla008584	Watermelon (97103) v1	lsiwmB078
Lsi10G010990	MELO3C014555	Melon (DHL92) v3.5.1	lsimeB072
Lsi10G010990	ClCG02G022780	Watermelon (Charleston Gray)	lsiwcgB054
Lsi10G010990	MELO3C014555.2	Melon (DHL92) v3.6.1	lsimedB074
Lsi10G010990	Cla97C02G048860	Watermelon (97103) v2	lsiwmbB052
Lsi10G010990	Bhi10G000190	Wax gourd	lsiwgoB079
Lsi10G010990	Cp4.1LG05g04730	Cucurbita pepo (Zucchini)	cpelsiB598
Lsi10G010990	Cp4.1LG16g08510	Cucurbita pepo (Zucchini)	cpelsiB227
Lsi10G010990	CsGy6G023130	Cucumber (Gy14) v2	cgyblsiB379
Lsi10G010990	Carg02682	Silver-seed gourd	carlsiB838

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi10G010990	Cucumber (Chinese Long) v3	cuclsiB440
Lsi10G010990	Silver-seed gourd	carlsiB471