Lsi07G010300 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi07G010300 vs. Swiss-Prot
Match: PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 3.3e-210
Identity = 355/508 (69.88%), Postives = 422/508 (83.07%), Query Frame = 1

Query: 74  GSSIIPSLIFCCFFFILLFPMLPATSPPDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAI 133
           G  ++  ++ C FF     P    +   DL QTQCL V +++F+ S+  TID VQQV +I
Sbjct: 15  GKQLLLLVLLCAFFSSSFIPFASCSITDDL-QTQCLKVSATEFAGSLKDTIDAVQQVASI 74

Query: 134 FSPFTKLLGDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLS 193
            S F    GD RL+ AISDCLDLLD SA++L+WSLSA+QN K K++STG LSSDL+TWLS
Sbjct: 75  LSQFANAFGDFRLANAISDCLDLLDFSADELNWSLSASQNQKGKNNSTGKLSSDLRTWLS 134

Query: 194 AAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQPA---EFPSWVK 253
           AA+VN +TC +GFEGTNSI++GL+S  + Q+TS + +LL+ V  N NQ     + PSWVK
Sbjct: 135 AALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLVQELLTQVHPNSNQQGPNGQIPSWVK 194

Query: 254 SEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFENVEIK 313
           ++DR LLQ + ++ DA VA DGTG+FTNV DAVLAAPD S++RYVIYIK+G Y ENVEIK
Sbjct: 195 TKDRKLLQADGVSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIK 254

Query: 314 KKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQ 373
           KKKWNLMMIGDG++ TIISGNR+F+DGWTTFRSATFAVSGRGFIARDITFENTAGP+KHQ
Sbjct: 255 KKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQ 314

Query: 374 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 433
           AVALRSDSDLSVF+RC IRGYQDTLYTHTMRQFYR+C+ISGTVDF+FGDATVVFQNC IL
Sbjct: 315 AVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQIL 374

Query: 434 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLK-PWVNTTATYLGRPWKEYSR 493
           AKKGLPNQKN+ITAQGRKDPN+PTG SIQFCNI+ADSDL+   VN+T TYLGRPWK YSR
Sbjct: 375 AKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSR 434

Query: 494 TIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQTSE 553
           T+IMQS++S+ IRPEGWLEWN DFALN+L+Y E+MNYGPGAGL  RV WPGY   N++++
Sbjct: 435 TVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQ 494

Query: 554 ATNFTVAQFIEGNMWLPSTGVKYTAGLG 578
           A N+TVAQFIEGN+WLPSTGVKYTA  G
Sbjct: 495 AKNYTVAQFIEGNLWLPSTGVKYTAEFG 521

BLAST of Lsi07G010300 vs. Swiss-Prot
Match: PME44_ARATH (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=PME44 PE=2 SV=2)

HSP 1 Score: 674.5 bits (1739), Expect = 1.1e-192
Identity = 323/521 (62.00%), Postives = 404/521 (77.54%), Query Frame = 1

Query: 84  CCFFFILLFPMLPATSPPDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAIFSPFTKLLGD 143
           C  +F++L  +    S  +  Q   L VP+S+F +SI + + V++QV +I S F    GD
Sbjct: 3   CLKYFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 62

Query: 144 LRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPETCM 203
            RL  A+SDCLDLLD S+E+L+WS SA++NPK K + TGD+ SD +TWLSAA+ N  TCM
Sbjct: 63  RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 122

Query: 204 DGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVK--------------------------- 263
           +GF+GT+ ++K LV+GS++QL S L +LL +V+                           
Sbjct: 123 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 182

Query: 264 -SNPNQPAEFPSWVKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYV 323
            ++ ++  +FP WV+ +DR LL+ N    D +VA DGTG+FT +MDA+  APD S  R+V
Sbjct: 183 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 242

Query: 324 IYIKKGVYFENVEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIA 383
           IYIKKG+Y ENVEIKKKKWN++M+GDGI+ T+ISGNR+FIDGWTTFRSATFAVSGRGF+A
Sbjct: 243 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLA 302

Query: 384 RDITFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDF 443
           RDITF+NTAGP+KHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF
Sbjct: 303 RDITFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDF 362

Query: 444 LFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNT 503
           +FGD TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P++NT
Sbjct: 363 IFGDGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNT 422

Query: 504 TATYLGRPWKEYSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKR 563
           T TYLGRPWK YSRT+ +++ MSD +RPEGWLEWNADFAL+TL+Y EFMNYGPG+GL+ R
Sbjct: 423 TRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSR 482

Query: 564 VNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGL 577
           V WPGYH  N + +A NFTV+QFI+GN+WLPSTGV ++ GL
Sbjct: 483 VKWPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523

BLAST of Lsi07G010300 vs. Swiss-Prot
Match: PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=PME32 PE=2 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 3.1e-192
Identity = 323/513 (62.96%), Postives = 410/513 (79.92%), Query Frame = 1

Query: 86  FFFILLFPMLPAT----SPPDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAIFSPFTKLL 145
           F F+++  +  A     S  DLVQ +CL VP  +F+ +  + +D + + +AI S F K  
Sbjct: 13  FLFLIIISLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKA 72

Query: 146 GDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPET 205
           G  R+S AI DC+DLLDS+AE+LSW +SA+Q+P  KD+STGD+ SDL+TW+SAA+ N +T
Sbjct: 73  GKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDT 132

Query: 206 CMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQP-----------------AE 265
           C+DGFEGTN IIK +V+G ++++ + + +LL+MV S P++P                 ++
Sbjct: 133 CLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSK 192

Query: 266 FPSWVKSEDRNLLQLNDLA-ADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVY 325
           FPSWVK  DR LLQ +++  ADA VAADGTG+FT + DAVLAAPD S +RYVI++K+GVY
Sbjct: 193 FPSWVKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVY 252

Query: 326 FENVEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENT 385
            ENVEIKKKKWN+MM+GDGI+ T+I+GNR+FIDGWTTFRSATFAVSGRGFIARDITF+NT
Sbjct: 253 VENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNT 312

Query: 386 AGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 445
           AGP+KHQAVA+RSD+DL VF+RC +RGYQDTLY H+MRQF+REC I+GTVDF+FGDAT V
Sbjct: 313 AGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAV 372

Query: 446 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRP 505
           FQ+C I AK+GLPNQKN+ITAQGRKDPN+PTGF+IQF NI+AD+DL   +NTTATYLGRP
Sbjct: 373 FQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRP 432

Query: 506 WKEYSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHR 565
           WK YSRT+ MQ+YMSDAI P GWLEWN +FAL+TLYY E+MN GPGA L +RV WPGYH 
Sbjct: 433 WKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHV 492

Query: 566 LNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGL 577
           LN ++EA NFTV+Q I+GN+WLPSTG+ + AGL
Sbjct: 493 LNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

BLAST of Lsi07G010300 vs. Swiss-Prot
Match: PME22_SOLLC (Pectinesterase 2.2 OS=Solanum lycopersicum GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.1e-128
Identity = 236/489 (48.26%), Postives = 321/489 (65.64%), Query Frame = 1

Query: 102 DLVQTQCLTVPSSQFSNSILSTIDVVQQV---MAIFSPFTKLLGDLRLSTAISDCLDLLD 161
           DL+  + +T  S   S  +   ++ V Q+   + + S     + D+R   A++DCL+LLD
Sbjct: 69  DLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQEGALTDCLELLD 128

Query: 162 SSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVS 221
            S + +S S++A       +H      ++ ++WLS  + N  TC+D  +   S  K +++
Sbjct: 129 QSVDLVSDSIAAIDKRTHSEH------ANAQSWLSGVLTNHVTCLDELD---SFTKAMIN 188

Query: 222 G-SINQLTSQLYDLLSMVKS--NPNQP------AEFPSWVKSEDRNLLQLN--DLAADAT 281
           G ++++L S+    L+M+ S   PN         + PSWV S DR L++ +  D+ A+A 
Sbjct: 189 GTNLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGANAV 248

Query: 282 VAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFENVEIKKKKWNLMMIGDGINDTI 341
           VA DGTG +  + +AV AAPD S  RYVIY+K+G+Y ENVE+  +K  LM++GDG++ TI
Sbjct: 249 VAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMHATI 308

Query: 342 ISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCR 401
           I+GN N +DG TTF SAT A  G+GFI +DI  +NTAGP KHQAVALR  +D SV  RCR
Sbjct: 309 ITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVINRCR 368

Query: 402 IRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGR 461
           I  YQDTLY H+ RQFYR+  ++GT+DF+FG+A VVFQ C ++A+K    Q+N +TAQGR
Sbjct: 369 IDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQQNMVTAQGR 428

Query: 462 KDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRPWKEYSRTIIMQSYMSDAIRPEGWL 521
            DPNQ TG SIQFCNI A SDL+P +    TYLGRPWK+YSRT++M+SY+   I P GW 
Sbjct: 429 TDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPAGWA 488

Query: 522 EWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPS 577
           EW+ DFAL TLYY EFMN GPGAG +KRV WPGYH +   +EA  FTVA+ I+G  WL S
Sbjct: 489 EWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSWLRS 548

BLAST of Lsi07G010300 vs. Swiss-Prot
Match: PME1_SOLLC (Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 454.5 bits (1168), Expect = 1.7e-126
Identity = 236/489 (48.26%), Postives = 315/489 (64.42%), Query Frame = 1

Query: 102 DLVQTQCLTVPSSQFSNSILSTIDVVQQVMAIFSPFTKL---LGDLRLSTAISDCLDLLD 161
           DL+  + +T  S   S  +   ++ V Q+        K+   + D+R   A++DCL+LLD
Sbjct: 65  DLISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHGALTDCLELLD 124

Query: 162 SSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVS 221
            S +  S S++A       +H+        ++WLS  + N  TC+D  +   S  K +++
Sbjct: 125 QSVDFASDSIAAIDKRSRSEHANA------QSWLSGVLTNHVTCLDELD---SFTKAMIN 184

Query: 222 GS-INQLTSQLYDLLSMVKSNPNQPAE--------FPSWVKSEDRNLLQLN--DLAADAT 281
           G+ + +L S+    L+M+ S   Q  +         PSWV S DR L++ +  D+ A+A 
Sbjct: 185 GTNLEELISRAKVALAMLASLTTQDEDVFMTVLGKMPSWVSSMDRKLMESSGKDIIANAV 244

Query: 282 VAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFENVEIKKKKWNLMMIGDGINDTI 341
           VA DGTGD+  + +AV AAPD S  RYVIY+K+G Y ENVE+   K NLM++GDG+  T 
Sbjct: 245 VAQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMIVGDGMYATT 304

Query: 342 ISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCR 401
           I+G+ N +DG TTFRSAT A  G+GFI +DI  +NTAGP K QAVALR  +D+SV  RCR
Sbjct: 305 ITGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGADMSVINRCR 364

Query: 402 IRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGR 461
           I  YQDTLY H+ RQFYR+  ++GTVDF+FG+A VVFQ C ++A+K    Q+N +TAQGR
Sbjct: 365 IDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQQNMVTAQGR 424

Query: 462 KDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRPWKEYSRTIIMQSYMSDAIRPEGWL 521
            DPNQ TG SIQFCNI A SDL+P +    TYLGRPWKEYSRT++M+SY+   I P GW 
Sbjct: 425 TDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWA 484

Query: 522 EWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPS 577
           EW+ DFAL TLYY EFMN GPGAG +KRV WPGYH +   ++A  FTVA+ I+G  WL S
Sbjct: 485 EWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRS 544

BLAST of Lsi07G010300 vs. TrEMBL
Match: A0A0A0L4S0_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G000060 PE=4 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 4.1e-276
Identity = 485/573 (84.64%), Postives = 514/573 (89.70%), Query Frame = 1

Query: 24  PFPTKRECIIQINALSPQPPPLKFPNLP-------TGPAFIYLISSPPKLRQTRIGRGSS 83
           P P       +     P P PLKF +L        T P F+ ++   P    T   +GSS
Sbjct: 7   PSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPI--HTGQAKGSS 66

Query: 84  IIPSLI---------FCCFFFILLFPML-PATSPPDLVQTQCLTVPSSQFSNSILSTIDV 143
           IIP L+         FCC   ILLFPML PA+SPPDLVQT+CL+VPSSQFSNS+LSTIDV
Sbjct: 67  IIPILMAKLTTQTLQFCCLL-ILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDV 126

Query: 144 VQQVMAIFSPFTKLLGDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSS 203
           V+QVMAIFSPF+KLLGD RLSTAISDCLDLLDSSA+QLSWSLSATQNPK K+HSTGDLSS
Sbjct: 127 VRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSS 186

Query: 204 DLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQPAEFP 263
           DLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSG +NQLTSQLYDLLSMVKS PNQP+EFP
Sbjct: 187 DLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPSEFP 246

Query: 264 SWVKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFEN 323
           SW+KSED+NLLQ+NDLAADATVAADGTGDFTNVMDAVLAAPDNS++RYVIYIKKGVY EN
Sbjct: 247 SWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLEN 306

Query: 324 VEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGP 383
           VEIKKKKWNLMMIGDGI+ TIISGNR+FIDGWTTFRSATFAVSGRGFIARDITFENTAG 
Sbjct: 307 VEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGA 366

Query: 384 QKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN 443
           +KHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN
Sbjct: 367 EKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN 426

Query: 444 CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRPWKE 503
           CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKP VNTTATYLGRPWKE
Sbjct: 427 CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKE 486

Query: 504 YSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQ 563
           YSRTIIMQSY+SDAIRPEGWLEWNA+FALNTL+YAEFMNYGPGAGLAKRVNWPGYHRLNQ
Sbjct: 487 YSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ 546

Query: 564 TSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN 580
           TSEATNFTVAQFIEGN+WLPSTGVKYTAGLGAN
Sbjct: 547 TSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576

BLAST of Lsi07G010300 vs. TrEMBL
Match: M5WFH0_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa004300mg PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 3.6e-208
Identity = 355/508 (69.88%), Postives = 422/508 (83.07%), Query Frame = 1

Query: 74  GSSIIPSLIFCCFFFILLFPMLPATSPPDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAI 133
           G  ++  ++ C FF     P    +   DL QTQCL V +++F+ S+  TID VQQV +I
Sbjct: 10  GKQLLLLVLLCAFFSSSFIPFASCSITDDL-QTQCLKVSATEFAGSLKDTIDAVQQVASI 69

Query: 134 FSPFTKLLGDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLS 193
            S F    GD RL+ AISDCLDLLD SA++L+WSLSA+QN K K++STG LSSDL+TWLS
Sbjct: 70  LSQFANAFGDFRLANAISDCLDLLDFSADELNWSLSASQNQKGKNNSTGKLSSDLRTWLS 129

Query: 194 AAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQPA---EFPSWVK 253
           AA+VN +TC +GFEGTNSI++GL+S  + Q+TS + +LL+ V  N NQ     + PSWVK
Sbjct: 130 AALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLVQELLTQVHPNSNQQGPNGQIPSWVK 189

Query: 254 SEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFENVEIK 313
           ++DR LLQ + ++ DA VA DGTG+FTNV DAVLAAPD S++RYVIYIK+G Y ENVEIK
Sbjct: 190 TKDRKLLQADGVSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYRENVEIK 249

Query: 314 KKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQ 373
           KKKWNLMMIGDG++ TIISGNR+F+DGWTTFRSATFAVSGRGFIARDITFENTAGP+KHQ
Sbjct: 250 KKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQ 309

Query: 374 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 433
           AVALRSDSDLSVF+RC IRGYQDTLYTHTMRQFYR+C+ISGTVDF+FGDATVVFQNC IL
Sbjct: 310 AVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQIL 369

Query: 434 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLK-PWVNTTATYLGRPWKEYSR 493
           AKKGLPNQKN+ITAQGRKDPN+PTG SIQFCNI+ADSDL+   VN+T TYLGRPWK YSR
Sbjct: 370 AKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSR 429

Query: 494 TIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQTSE 553
           T+IMQS++S+ IRPEGWLEWN DFALN+L+Y E+MNYGPGAGL  RV WPGY   N++++
Sbjct: 430 TVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQ 489

Query: 554 ATNFTVAQFIEGNMWLPSTGVKYTAGLG 578
           A N+TVAQFIEGN+WLPSTGVKYTA  G
Sbjct: 490 AKNYTVAQFIEGNLWLPSTGVKYTAEFG 516

BLAST of Lsi07G010300 vs. TrEMBL
Match: R9QPU3_GOSHI (Pectinesterase OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 1.8e-207
Identity = 350/501 (69.86%), Postives = 417/501 (83.23%), Query Frame = 1

Query: 97  ATSPPDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAIFSPFTKLLGDLRLSTAISDCLDL 156
           ++S  + ++T+CL VP+++F  S+ +TID +++  ++ S F     D RLS AISDCLDL
Sbjct: 24  SSSTNEFLETECLKVPATEFIGSLKTTIDAIRKATSVVSQFGGFFHDFRLSNAISDCLDL 83

Query: 157 LDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 216
           LDSSA++LSW++SA+QNP  KD+STGDLSSDL+TWLSAA+VN +TC+DGFEGTNS++K +
Sbjct: 84  LDSSADELSWTMSASQNPNAKDNSTGDLSSDLRTWLSAAMVNQQTCIDGFEGTNSMVKTV 143

Query: 217 VSGSINQLTSQLYDLLSMVKSNPNQ--------------------PAEFPSWVKSEDRNL 276
           VSGS+NQ+TS + +LL MV   PN                      + FP W K EDR L
Sbjct: 144 VSGSLNQITSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGGGHPGQSRFPVWFKREDRRL 203

Query: 277 LQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFENVEIKKKKWNL 336
            Q+N + A+  VAADG+G+FT +MDAV  APD SL RYVIYIKKG+Y ENVEIKKKKWNL
Sbjct: 204 SQINGVTANVVVAADGSGNFTRIMDAVETAPDKSLNRYVIYIKKGLYKENVEIKKKKWNL 263

Query: 337 MMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 396
           MMIGDG++ T+ISGNR+FIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS
Sbjct: 264 MMIGDGMDVTVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 323

Query: 397 DSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLP 456
           DSDLSVFFRC I+GYQD+LYTHTMRQFYREC+I+GTVDF+FGD  V+FQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAIKGYQDSLYTHTMRQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLP 383

Query: 457 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRPWKEYSRTIIMQSY 516
           +QKNTITAQGRKDPNQPTGFSIQFCNISAD+DL P VN+T TYLGRPWK YSRTIIMQSY
Sbjct: 384 SQKNTITAQGRKDPNQPTGFSIQFCNISADTDLLPSVNSTPTYLGRPWKLYSRTIIMQSY 443

Query: 517 MSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVA 576
           +SDAIRP+GWLEWN DFAL+TLYYAE+MN GPGA L++RV WPGYH LN +++A NFTVA
Sbjct: 444 ISDAIRPQGWLEWNQDFALDTLYYAEYMNNGPGASLSERVKWPGYHVLNNSAQAVNFTVA 503

Query: 577 QFIEGNMWLPSTGVKYTAGLG 578
           QFIEG++WLPSTGVKYT+G G
Sbjct: 504 QFIEGDLWLPSTGVKYTSGFG 524

BLAST of Lsi07G010300 vs. TrEMBL
Match: V4UJ53_9ROSI (Pectinesterase OS=Citrus clementina GN=CICLE_v10007993mg PE=4 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 2.3e-207
Identity = 353/522 (67.62%), Postives = 422/522 (80.84%), Query Frame = 1

Query: 81  LIFC-CFFFILLFPMLPATSPP----DLVQTQCLTVPSSQFSNSILSTIDVVQQVMAIFS 140
           L FC  F FI L   +  +S      +  Q +CL VP+S F +S+ +T+D +QQV+ + S
Sbjct: 2   LNFCKLFIFIFLVSAVSLSSSSNAGSEYSQAECLKVPASSFMSSLKTTVDAIQQVVPVIS 61

Query: 141 PFTKLLGDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAA 200
            F  L GD RL+ AISDCLDLLD SA++L+WS+SA+QNP  + + +GDLSSDL+TWLSAA
Sbjct: 62  NFANLFGDFRLTNAISDCLDLLDFSADELTWSISASQNPTGRHNGSGDLSSDLRTWLSAA 121

Query: 201 VVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQ-------------- 260
           ++N ETC+DGF+GTNSI+KG+VSGS+NQ++S + +LL+MV  +P+Q              
Sbjct: 122 LINQETCIDGFDGTNSIVKGVVSGSLNQISSSVQELLTMVHPSPSQWSNEGSSSNSGGKS 181

Query: 261 -------PAEFPSWVKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRY 320
                    +FP W K EDR LL +N + AD  VAADGTG+FT +MDAVLAA D S++R+
Sbjct: 182 RGGGGKSSGQFPHWFKREDRKLLLVNGVQADVVVAADGTGNFTKIMDAVLAAEDYSMKRF 241

Query: 321 VIYIKKGVYFENVEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFI 380
           VIYIK+GVY ENVEIKKKKWNLMM+GDGI+ TIISGNRNF+DGWTTFRSATFAVSGRGFI
Sbjct: 242 VIYIKRGVYKENVEIKKKKWNLMMVGDGIDATIISGNRNFMDGWTTFRSATFAVSGRGFI 301

Query: 381 ARDITFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVD 440
           ARDITFENTAGP+KHQAVALRSDSDLSV+FRC I+GYQD+LYTHTMRQF+REC+ISGTVD
Sbjct: 302 ARDITFENTAGPEKHQAVALRSDSDLSVYFRCAIKGYQDSLYTHTMRQFFRECKISGTVD 361

Query: 441 FLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVN 500
           F+FGDAT +FQNC ILAKKGL NQKNTITA GRKDPN+PTGFSIQFCNI+ADSDL P+VN
Sbjct: 362 FIFGDATAMFQNCQILAKKGLDNQKNTITAHGRKDPNEPTGFSIQFCNITADSDLLPFVN 421

Query: 501 TTATYLGRPWKEYSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAK 560
           +T TYLGRPWK YSRT+ MQSYMS+ IRP+GWLEWN DFAL+TLYY E+ NYGPG GLA 
Sbjct: 422 STETYLGRPWKLYSRTVFMQSYMSNVIRPKGWLEWNNDFALDTLYYGEYKNYGPGGGLAT 481

Query: 561 RVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGL 577
           R+ WPGYH LN   +A NFTVAQF+EGN+WLPSTGVKYTAGL
Sbjct: 482 RITWPGYHILNNAVQANNFTVAQFLEGNLWLPSTGVKYTAGL 523

BLAST of Lsi07G010300 vs. TrEMBL
Match: A0A0B0MDS0_GOSAR (Pectinesterase OS=Gossypium arboreum GN=F383_36646 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 3.1e-207
Identity = 349/501 (69.66%), Postives = 417/501 (83.23%), Query Frame = 1

Query: 97  ATSPPDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAIFSPFTKLLGDLRLSTAISDCLDL 156
           ++S  + ++T+CL VP+++F  S+ +TID +++  ++ S F     D RLS AI DCLDL
Sbjct: 24  SSSTNEFLETECLKVPATEFIGSLKTTIDAIRKATSVVSQFGGFFHDFRLSNAIFDCLDL 83

Query: 157 LDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 216
           LDSSA++LSW++SA+QNP  KD+STGDLSSDL+TWLSAA+VN +TC+DGFEGTNS++K +
Sbjct: 84  LDSSADELSWTMSASQNPNAKDNSTGDLSSDLRTWLSAAMVNQQTCIDGFEGTNSMVKTV 143

Query: 217 VSGSINQLTSQLYDLLSMVKSNPNQPAE--------------------FPSWVKSEDRNL 276
           VSGS+NQ+TS + +LL MV   PN  +                     FP W K EDR L
Sbjct: 144 VSGSLNQITSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGGGHPGQNRFPVWFKREDRRL 203

Query: 277 LQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFENVEIKKKKWNL 336
           LQ+N + A+  VAADG+G+FT +MDAV  APD S+ RYVIYIKKG+Y ENVEIKKKKWNL
Sbjct: 204 LQINGVTANVVVAADGSGNFTRIMDAVETAPDKSMNRYVIYIKKGLYKENVEIKKKKWNL 263

Query: 337 MMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 396
           MMIGDG++ T+ISGNR+FIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS
Sbjct: 264 MMIGDGMDVTVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 323

Query: 397 DSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLP 456
           DSDLSVFFRC I+GYQD+LYTHTMRQFYREC+I+GTVDF+FGD  V+FQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAIKGYQDSLYTHTMRQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLP 383

Query: 457 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRPWKEYSRTIIMQSY 516
           +QKNTITAQGRKDPNQPTGFSIQFCNISAD+DL P VN+T TYLGRPWK YSRTIIMQSY
Sbjct: 384 SQKNTITAQGRKDPNQPTGFSIQFCNISADTDLLPSVNSTPTYLGRPWKLYSRTIIMQSY 443

Query: 517 MSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVA 576
           +SDAIRP+GWLEWN DFAL+TLYYAE+MN GPGA L++RV WPGYH LN +++A NFTVA
Sbjct: 444 ISDAIRPQGWLEWNQDFALDTLYYAEYMNNGPGASLSERVKWPGYHVLNNSAQAVNFTVA 503

Query: 577 QFIEGNMWLPSTGVKYTAGLG 578
           QFIEG++WLPSTGVKYT+G G
Sbjct: 504 QFIEGDLWLPSTGVKYTSGFG 524

BLAST of Lsi07G010300 vs. TAIR10
Match: AT4G33220.1 (AT4G33220.1 pectin methylesterase 44)

HSP 1 Score: 674.5 bits (1739), Expect = 5.9e-194
Identity = 323/521 (62.00%), Postives = 404/521 (77.54%), Query Frame = 1

Query: 84  CCFFFILLFPMLPATSPPDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAIFSPFTKLLGD 143
           C  +F++L  +    S  +  Q   L VP+S+F +SI + + V++QV +I S F    GD
Sbjct: 3   CLKYFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 62

Query: 144 LRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPETCM 203
            RL  A+SDCLDLLD S+E+L+WS SA++NPK K + TGD+ SD +TWLSAA+ N  TCM
Sbjct: 63  RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 122

Query: 204 DGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVK--------------------------- 263
           +GF+GT+ ++K LV+GS++QL S L +LL +V+                           
Sbjct: 123 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 182

Query: 264 -SNPNQPAEFPSWVKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYV 323
            ++ ++  +FP WV+ +DR LL+ N    D +VA DGTG+FT +MDA+  APD S  R+V
Sbjct: 183 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 242

Query: 324 IYIKKGVYFENVEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIA 383
           IYIKKG+Y ENVEIKKKKWN++M+GDGI+ T+ISGNR+FIDGWTTFRSATFAVSGRGF+A
Sbjct: 243 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLA 302

Query: 384 RDITFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDF 443
           RDITF+NTAGP+KHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF
Sbjct: 303 RDITFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDF 362

Query: 444 LFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNT 503
           +FGD TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P++NT
Sbjct: 363 IFGDGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNT 422

Query: 504 TATYLGRPWKEYSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKR 563
           T TYLGRPWK YSRT+ +++ MSD +RPEGWLEWNADFAL+TL+Y EFMNYGPG+GL+ R
Sbjct: 423 TRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSR 482

Query: 564 VNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGL 577
           V WPGYH  N + +A NFTV+QFI+GN+WLPSTGV ++ GL
Sbjct: 483 VKWPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523

BLAST of Lsi07G010300 vs. TAIR10
Match: AT3G43270.1 (AT3G43270.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 672.9 bits (1735), Expect = 1.7e-193
Identity = 323/513 (62.96%), Postives = 410/513 (79.92%), Query Frame = 1

Query: 86  FFFILLFPMLPAT----SPPDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAIFSPFTKLL 145
           F F+++  +  A     S  DLVQ +CL VP  +F+ +  + +D + + +AI S F K  
Sbjct: 13  FLFLIIISLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKA 72

Query: 146 GDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPET 205
           G  R+S AI DC+DLLDS+AE+LSW +SA+Q+P  KD+STGD+ SDL+TW+SAA+ N +T
Sbjct: 73  GKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDT 132

Query: 206 CMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQP-----------------AE 265
           C+DGFEGTN IIK +V+G ++++ + + +LL+MV S P++P                 ++
Sbjct: 133 CLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSK 192

Query: 266 FPSWVKSEDRNLLQLNDLA-ADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVY 325
           FPSWVK  DR LLQ +++  ADA VAADGTG+FT + DAVLAAPD S +RYVI++K+GVY
Sbjct: 193 FPSWVKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVY 252

Query: 326 FENVEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENT 385
            ENVEIKKKKWN+MM+GDGI+ T+I+GNR+FIDGWTTFRSATFAVSGRGFIARDITF+NT
Sbjct: 253 VENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNT 312

Query: 386 AGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 445
           AGP+KHQAVA+RSD+DL VF+RC +RGYQDTLY H+MRQF+REC I+GTVDF+FGDAT V
Sbjct: 313 AGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAV 372

Query: 446 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRP 505
           FQ+C I AK+GLPNQKN+ITAQGRKDPN+PTGF+IQF NI+AD+DL   +NTTATYLGRP
Sbjct: 373 FQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRP 432

Query: 506 WKEYSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHR 565
           WK YSRT+ MQ+YMSDAI P GWLEWN +FAL+TLYY E+MN GPGA L +RV WPGYH 
Sbjct: 433 WKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHV 492

Query: 566 LNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGL 577
           LN ++EA NFTV+Q I+GN+WLPSTG+ + AGL
Sbjct: 493 LNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

BLAST of Lsi07G010300 vs. TAIR10
Match: AT3G05620.1 (AT3G05620.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 441.4 bits (1134), Expect = 8.4e-124
Identity = 226/456 (49.56%), Postives = 299/456 (65.57%), Query Frame = 1

Query: 145 RLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSD------------LKTWL 204
           R   AI DC +L+  S  +L+WS+        K H  G +  D            LKTWL
Sbjct: 100 REQVAIEDCKELVGFSVTELAWSMLEMN----KLHGGGGIDLDDGSHDAAAAGGNLKTWL 159

Query: 205 SAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMV--------KSNPNQPA- 264
           SAA+ N +TC++GFEGT    + L+ GS+ Q+T  + ++L M         K++ N+   
Sbjct: 160 SAAMSNQDTCLEGFEGTERKYEELIKGSLRQVTQLVSNVLDMYTQLNALPFKASRNESVI 219

Query: 265 EFPSWVKSEDRNLLQLND---LAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKK 324
             P W+   D +L+  +D   +  +  VA DG G +  + +A+  AP++S +RYVIY+KK
Sbjct: 220 ASPEWLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKK 279

Query: 325 GVYFENVEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITF 384
           GVY EN+++KKKK N+M++GDGI  TII+G+RNF+ G TTFR+AT AVSGRGFIA+DITF
Sbjct: 280 GVYKENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITF 339

Query: 385 ENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDA 444
            NTAGPQ  QAVALR DSD S F+RC + GYQDTLY H++RQFYR+C+I GT+DF+FG+ 
Sbjct: 340 RNTAGPQNRQAVALRVDSDQSAFYRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNG 399

Query: 445 TVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYL 504
             V QNC I  +  LP QK TITAQGRK PNQ TGF IQ   + A         T  TYL
Sbjct: 400 AAVLQNCKIYTRVPLPLQKVTITAQGRKSPNQNTGFVIQNSYVLA---------TQPTYL 459

Query: 505 GRPWKEYSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPG 564
           GRPWK YSRT+ M +YMS  ++P GWLEW  +FAL+TL+Y E+ N GPG   + RV WPG
Sbjct: 460 GRPWKLYSRTVYMNTYMSQLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPG 519

Query: 565 YHRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGL 577
           YH +++ + A +FTV  FI+G  WLP+TGV +TAGL
Sbjct: 520 YHIMDKRT-ALSFTVGSFIDGRRWLPATGVTFTAGL 541

BLAST of Lsi07G010300 vs. TAIR10
Match: AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)

HSP 1 Score: 427.9 bits (1099), Expect = 9.7e-120
Identity = 223/455 (49.01%), Postives = 295/455 (64.84%), Query Frame = 1

Query: 145 RLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPETCMD 204
           R+ +A   CL+LLD S + L+ +LS+     V   S  +  SD+ TWLS+A+ N +TC D
Sbjct: 136 RVRSAYDSCLELLDDSVDALTRALSS-----VVVVSGDESHSDVMTWLSSAMTNHDTCTD 195

Query: 205 GF---EGTNSIIKGLVSGSINQLTSQLYDLLSM----VKSNPNQPA-------------E 264
           GF   EG    +K  V G++  L+  + + L++    VK     P              E
Sbjct: 196 GFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE 255

Query: 265 FPSWVKSEDRNLL--QLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGV 324
            P+W+K EDR LL    + + AD TV+ DG+G F  + +A+  AP++S +R+VIY+K G 
Sbjct: 256 LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGR 315

Query: 325 YFE-NVEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFE 384
           Y E N+++ +KK NLM IGDG   T+I+G ++  D  TTF +ATFA +G GFI RD+TFE
Sbjct: 316 YEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFE 375

Query: 385 NTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 444
           N AGP KHQAVALR   D +V +RC I GYQD LY H+ RQF+REC+I GTVDF+FG+A 
Sbjct: 376 NYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAA 435

Query: 445 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLG 504
           V+ Q+C+I A+K +  QK TITAQ RKDPNQ TG SI  C + A  DL+    +  TYLG
Sbjct: 436 VILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLG 495

Query: 505 RPWKEYSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGY 564
           RPWK YSR + M S M D I P GWLEWN  FAL++LYY E+MN G G+G+ +RV WPGY
Sbjct: 496 RPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGY 555

Query: 565 HRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGL 577
           H +  T EA+ FTVAQFI G+ WLPSTGV + +GL
Sbjct: 556 HVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of Lsi07G010300 vs. TAIR10
Match: AT3G49220.1 (AT3G49220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 426.4 bits (1095), Expect = 2.8e-119
Identity = 225/507 (44.38%), Postives = 315/507 (62.13%), Query Frame = 1

Query: 101 PDLVQTQCLTVPSSQFSNSILSTIDVVQQVM------AIFSPFTKLLGDL--RLSTAISD 160
           P+L     +  P S  ++S    I V   +       A++S  +    D+  R  +A   
Sbjct: 97  PELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDS 156

Query: 161 CLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGT-NS 220
           C++LLD S + LS +LS+  +   K         D+ TWLSAA+ N +TC +GF+G  + 
Sbjct: 157 CVELLDDSVDALSRALSSVVSSSAKPQ-------DVTTWLSAALTNHDTCTEGFDGVDDG 216

Query: 221 IIKGLVSGSINQLTSQLYDLLSMVKSN-------------------PNQPAEFPSWVKSE 280
            +K  ++ ++  L+  + + L++  ++                     +  +FP W++ +
Sbjct: 217 GVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEKFPRWMRPK 276

Query: 281 DRNLLQL--NDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFE-NVEI 340
           +R +L++  + + AD  V+ DG G    + +A+  AP NS +R +IY+K G Y E N+++
Sbjct: 277 EREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKV 336

Query: 341 KKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKH 400
            +KK NLM +GDG   T+ISG ++  D  TTF +A+FA +G GFIARDITFEN AGP KH
Sbjct: 337 GRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKH 396

Query: 401 QAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSI 460
           QAVALR  +D +V +RC I GYQDTLY H+ RQF+REC I GTVDF+FG+A VV QNCSI
Sbjct: 397 QAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSI 456

Query: 461 LAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRPWKEYSR 520
            A+K +  QKNTITAQ RKDPNQ TG SI    + A SDL+    +T TYLGRPWK +SR
Sbjct: 457 YARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSR 516

Query: 521 TIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQTSE 577
           T+ M SY+   +   GWLEWN  FAL+TLYY E++N GPG+GL +RV+WPGY  +N T+E
Sbjct: 517 TVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAE 576

BLAST of Lsi07G010300 vs. NCBI nr
Match: gi|659095151|ref|XP_008448427.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo])

HSP 1 Score: 1000.0 bits (2584), Expect = 1.7e-288
Identity = 510/578 (88.24%), Postives = 530/578 (91.70%), Query Frame = 1

Query: 21  SDKPFPTKRECIIQINALSPQPPPLK--FPNLPTGPAFIY-------LISSPPKLRQTRI 80
           SDKPFPTKREC IQINALSPQPP  K  FP+LPTG  FIY       L+  P    Q   
Sbjct: 4   SDKPFPTKRECSIQINALSPQPPAFKVPFPHLPTG-LFIYFTDFVLILLHHPIHTGQ--- 63

Query: 81  GRGSSIIPSLI---------FCCFFFILLFPML-PATSPPDLVQTQCLTVPSSQFSNSIL 140
           G+GSSIIP L+         FCC F ILLFPML PA+SP DLVQT+CL+VPSSQFSNS+L
Sbjct: 64  GKGSSIIPILMAKLTTQILQFCCIF-ILLFPMLLPASSPADLVQTECLSVPSSQFSNSLL 123

Query: 141 STIDVVQQVMAIFSPFTKLLGDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHST 200
           STIDVV+QVMAIFSPF+KLLGD RLSTAISDCLDLLDSSA+QLSWSLSATQNPK KDHST
Sbjct: 124 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKDHST 183

Query: 201 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQ 260
           GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSG INQLTSQLYDLLSMVKS PNQ
Sbjct: 184 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGINQLTSQLYDLLSMVKSIPNQ 243

Query: 261 PAEFPSWVKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKG 320
           P+EFPSW+KSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPD S +RYVIYIKKG
Sbjct: 244 PSEFPSWLKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDKSTRRYVIYIKKG 303

Query: 321 VYFENVEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFE 380
           VY ENVEIKKKKWNLMMIGDGI+ TIISGNR+FIDGWTTFRSATFAVSGRGFIARDITFE
Sbjct: 304 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 363

Query: 381 NTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 440
           NTAG +KHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT
Sbjct: 364 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 423

Query: 441 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLG 500
           VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKP VNTTATYLG
Sbjct: 424 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 483

Query: 501 RPWKEYSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGY 560
           RPWKEYSRTIIMQSY+SDAIRPEGWLEWN +FALNTL+YAEFMNYGPGAGLAKRVNWPGY
Sbjct: 484 RPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFALNTLFYAEFMNYGPGAGLAKRVNWPGY 543

Query: 561 HRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN 580
           HRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN
Sbjct: 544 HRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN 576

BLAST of Lsi07G010300 vs. NCBI nr
Match: gi|778674515|ref|XP_004146188.2| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus])

HSP 1 Score: 958.4 bits (2476), Expect = 5.8e-276
Identity = 485/573 (84.64%), Postives = 514/573 (89.70%), Query Frame = 1

Query: 24  PFPTKRECIIQINALSPQPPPLKFPNLP-------TGPAFIYLISSPPKLRQTRIGRGSS 83
           P P       +     P P PLKF +L        T P F+ ++   P    T   +GSS
Sbjct: 7   PSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPI--HTGQAKGSS 66

Query: 84  IIPSLI---------FCCFFFILLFPML-PATSPPDLVQTQCLTVPSSQFSNSILSTIDV 143
           IIP L+         FCC   ILLFPML PA+SPPDLVQT+CL+VPSSQFSNS+LSTIDV
Sbjct: 67  IIPILMAKLTTQTLQFCCLL-ILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDV 126

Query: 144 VQQVMAIFSPFTKLLGDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSS 203
           V+QVMAIFSPF+KLLGD RLSTAISDCLDLLDSSA+QLSWSLSATQNPK K+HSTGDLSS
Sbjct: 127 VRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSS 186

Query: 204 DLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQPAEFP 263
           DLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSG +NQLTSQLYDLLSMVKS PNQP+EFP
Sbjct: 187 DLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPSEFP 246

Query: 264 SWVKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFEN 323
           SW+KSED+NLLQ+NDLAADATVAADGTGDFTNVMDAVLAAPDNS++RYVIYIKKGVY EN
Sbjct: 247 SWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLEN 306

Query: 324 VEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGP 383
           VEIKKKKWNLMMIGDGI+ TIISGNR+FIDGWTTFRSATFAVSGRGFIARDITFENTAG 
Sbjct: 307 VEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGA 366

Query: 384 QKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN 443
           +KHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN
Sbjct: 367 EKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN 426

Query: 444 CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRPWKE 503
           CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKP VNTTATYLGRPWKE
Sbjct: 427 CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKE 486

Query: 504 YSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQ 563
           YSRTIIMQSY+SDAIRPEGWLEWNA+FALNTL+YAEFMNYGPGAGLAKRVNWPGYHRLNQ
Sbjct: 487 YSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ 546

Query: 564 TSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN 580
           TSEATNFTVAQFIEGN+WLPSTGVKYTAGLGAN
Sbjct: 547 TSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576

BLAST of Lsi07G010300 vs. NCBI nr
Match: gi|694388007|ref|XP_009369733.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B [Pyrus x bretschneideri])

HSP 1 Score: 741.5 bits (1913), Expect = 1.1e-210
Identity = 361/502 (71.91%), Postives = 418/502 (83.27%), Query Frame = 1

Query: 86  FFFILLFPMLPATSP------PDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAIFSPFTK 145
           FF +LL     +  P       D +Q++CL VP+S+F+ S+  TID VQQV  I S F  
Sbjct: 8   FFLVLLCVCCKSRIPFASCSFTDDIQSECLKVPASEFAGSLRDTIDAVQQVATILSQFAN 67

Query: 146 LLGDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNP 205
             GD RLS AISDCLDLLD SA++L+WSLSA+QN K K +STG LSSDL+TWLSAA+VN 
Sbjct: 68  AFGDFRLSNAISDCLDLLDFSADELNWSLSASQNQKGKSNSTGKLSSDLRTWLSAALVNQ 127

Query: 206 ETCMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQPA---EFPSWVKSEDRNL 265
           +TC DGFEGTNSI+KGLVS  +NQ+TS + DLL+ V  N +      + P+WVK+EDR L
Sbjct: 128 DTCSDGFEGTNSIVKGLVSTGLNQVTSLVQDLLTQVHPNSDHQGPNGQIPTWVKTEDRKL 187

Query: 266 LQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFENVEIKKKKWNL 325
           LQ + +  DA VA DGTG+FTNVMDAVLAAPD S+ RYVIYIKKG Y ENVEIKKKKWNL
Sbjct: 188 LQADGVNVDAVVAQDGTGNFTNVMDAVLAAPDYSMSRYVIYIKKGTYKENVEIKKKKWNL 247

Query: 326 MMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 385
           MMIGDG++ TIISGNR++IDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS
Sbjct: 248 MMIGDGMDATIISGNRSYIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 307

Query: 386 DSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLP 445
           DSDLSVF+RC IRGYQDTLY HTMRQFYR+C+ISGTVDF+FGDATVVFQNC ILAKKGLP
Sbjct: 308 DSDLSVFYRCEIRGYQDTLYAHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLP 367

Query: 446 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPWVNTTATYLGRPWKEYSRTIIMQSY 505
           NQKN++TAQGRKDPN+PTG SIQFCNI+AD+DL P+VN+T TYLGRPWK YSRT+IMQS+
Sbjct: 368 NQKNSVTAQGRKDPNEPTGISIQFCNITADADLLPFVNSTFTYLGRPWKLYSRTVIMQSF 427

Query: 506 MSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVA 565
           +S+AIRPEGWLEWNADFAL+TL Y E+MNYGPGAGL  RV WPGY   N++++  N+TVA
Sbjct: 428 LSNAIRPEGWLEWNADFALSTLSYGEYMNYGPGAGLGSRVKWPGYQVFNESTQVKNYTVA 487

Query: 566 QFIEGNMWLPSTGVKYTAGLGA 579
           QFIEGN+WLP TGVKYTAG GA
Sbjct: 488 QFIEGNLWLPPTGVKYTAGFGA 509

BLAST of Lsi07G010300 vs. NCBI nr
Match: gi|645274002|ref|XP_008242144.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B [Prunus mume])

HSP 1 Score: 738.4 bits (1905), Expect = 9.5e-210
Identity = 359/508 (70.67%), Postives = 423/508 (83.27%), Query Frame = 1

Query: 74  GSSIIPSLIFCCFFFILLFPMLPATSPPDLVQTQCLTVPSSQFSNSILSTIDVVQQVMAI 133
           G  ++  ++ C FF     P    +   DL QT CL VP+S+F+ S+  TID VQQV +I
Sbjct: 10  GKQLLLLVLLCAFFSSSFIPFASCSITDDL-QTGCLKVPASEFAGSLKDTIDAVQQVASI 69

Query: 134 FSPFTKLLGDLRLSTAISDCLDLLDSSAEQLSWSLSATQNPKVKDHSTGDLSSDLKTWLS 193
            SPF    GD RLS AISDCLDLLD SA++L+WS+SA+QN K K++STG LSSDL+TWLS
Sbjct: 70  LSPFANAFGDFRLSNAISDCLDLLDFSADELNWSISASQNQKGKNNSTGKLSSDLRTWLS 129

Query: 194 AAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYDLLSMVKSNPNQPA---EFPSWVK 253
           AA+VN +TC +GFEGTNSI++GLVS  ++Q+TS + +LL+ V  N NQ     + PSWVK
Sbjct: 130 AALVNQDTCSNGFEGTNSIVQGLVSAGLDQVTSLVQELLTQVHPNSNQQGPNGQIPSWVK 189

Query: 254 SEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDNSLQRYVIYIKKGVYFENVEIK 313
           +EDR LLQ + ++ DA VA DGTG+FTNV DAVLAAPD S++RYVIYIK+G Y ENVEIK
Sbjct: 190 TEDRKLLQADGVSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIK 249

Query: 314 KKKWNLMMIGDGINDTIISGNRNFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQ 373
           KKKWNLMMIGDG++ TIISGNRNF+DGWTTFRSATFAVSGRGFIARDITFENTAGP+KHQ
Sbjct: 250 KKKWNLMMIGDGMDATIISGNRNFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQ 309

Query: 374 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 433
           AVALRSDSDLSVF+RC IRGYQDTLYTHTMRQFYR+C+ISGTVDF+FGDATVVFQNC IL
Sbjct: 310 AVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQIL 369

Query: 434 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLK-PWVNTTATYLGRPWKEYSR 493
           AKKGLPNQKN+ITAQGRKD N+PTG SIQFCNI+ADSDL+   VN+T TYLGRPWK YSR
Sbjct: 370 AKKGLPNQKNSITAQGRKDLNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSR 429

Query: 494 TIIMQSYMSDAIRPEGWLEWNADFALNTLYYAEFMNYGPGAGLAKRVNWPGYHRLNQTSE 553
           T+IMQS++S+ IRPEGWLEWN DFALN+L+Y E+MNYGPGAGL  RV WPGY   N++++
Sbjct: 430 TVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQ 489

Query: 554 ATNFTVAQFIEGNMWLPSTGVKYTAGLG 578
           A N+TVAQFIEGN+WLPSTGVKYTA  G
Sbjct: 490 AKNYTVAQFIEGNLWLPSTGVKYTADFG 516

BLAST of Lsi07G010300 vs. NCBI nr
Match: gi|658005299|ref|XP_008337790.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B [Malus domestica])

HSP 1 Score: 733.8 bits (1893), Expect = 2.3e-208
Identity = 351/478 (73.43%), Postives = 408/478 (85.36%), Query Frame = 1

Query: 104 VQTQCLTVPSSQFSNSILSTIDVVQQVMAIFSPFTKLLGDLRLSTAISDCLDLLDSSAEQ 163
           + ++CL VP+S+F+ S+  TI  VQQV +I S F    GD RLS AISDCLDLLD SA++
Sbjct: 32  IPSECLKVPASEFAGSLRDTIGAVQQVASILSQFANAFGDFRLSNAISDCLDLLDFSADE 91

Query: 164 LSWSLSATQNPKVKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGSINQ 223
           L+WSLSA+QNP+ KD+STG LSSDL+TWLSAA+VN +TC DGFEGTNSI+KGLVS  +NQ
Sbjct: 92  LNWSLSASQNPEGKDNSTGKLSSDLRTWLSAALVNQDTCSDGFEGTNSIVKGLVSTGLNQ 151

Query: 224 LTSQLYDLLSMVKSNPNQPA---EFPSWVKSEDRNLLQLNDLAADATVAADGTGDFTNVM 283
           +TS +  LL+ V  N +      E PSWVK+EDR LLQ   +  DA VA DGTG+FTNVM
Sbjct: 152 VTSLVQGLLTQVHPNTDHHGPNGEIPSWVKTEDRKLLQAGGVNVDAVVAQDGTGNFTNVM 211

Query: 284 DAVLAAPDNSLQRYVIYIKKGVYFENVEIKKKKWNLMMIGDGINDTIISGNRNFIDGWTT 343
           DAVLAAPD  + RYVIYIKKG Y ENVEIKKKKWNLMMIGDG++ TIISGNRNF+DGWTT
Sbjct: 212 DAVLAAPDERMTRYVIYIKKGTYKENVEIKKKKWNLMMIGDGMDATIISGNRNFVDGWTT 271

Query: 344 FRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTM 403
           FRSATFAVSGRGFIARD+TFENTAGP+KHQAVALRSDSDLSVF+RC+IRGYQDTLY H M
Sbjct: 272 FRSATFAVSGRGFIARDLTFENTAGPEKHQAVALRSDSDLSVFYRCQIRGYQDTLYAHAM 331

Query: 404 RQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQF 463
           RQFYR+C+ISGTVDF+FGDATVVFQNC ILAKKGLPNQKN++TAQGRKDPN+PTG SIQF
Sbjct: 332 RQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSVTAQGRKDPNEPTGISIQF 391

Query: 464 CNISADSDLKPWVNTTATYLGRPWKEYSRTIIMQSYMSDAIRPEGWLEWNADFALNTLYY 523
           CNI+AD+DL P VN+T+TYLGRPWK YSRT+IMQS++S+AIRPEGWLEWNADFAL+TL Y
Sbjct: 392 CNITADADLLPLVNSTSTYLGRPWKLYSRTVIMQSFLSNAIRPEGWLEWNADFALSTLSY 451

Query: 524 AEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGLGA 579
            E+MNYGPGAGL  RV WPGY   N++++  N+TVAQFIEG++WLP TGVKY AGLGA
Sbjct: 452 GEYMNYGPGAGLESRVKWPGYRVFNESTQVQNYTVAQFIEGDLWLPPTGVKYIAGLGA 509