Carg01161 (gene) Silver-seed gourd

NameCarg01161
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPectinesterase
LocationCucurbita_argyrosperma_scaffold_002 : 1258454 .. 1260821 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCGGCGGCGGATCTTGAACAAACAGAGTGCTTAAAAGTCCCCTCGTCGGAGTTCTCCAACTCTGTTCTCTCCACCATCGGCGTCGTGCGCCAGGTCATGCTCATTTTCTCTCCATTCACTAAATTTCTCGGTGATTCCCGCCTTTCCACCGCCATTTCCGACTGTCTTGATTTGCTGGATTCTTCTTCCGACCAGTTGAGCTGGTCGCTCTCCGCCGCCCAAAATCCGAAGGGTAAACGGTCTTTGTCGCCAAAACATAATAAATTTCGTTTTTGCATATTAACCCACATCTGTTTTCGTTTTGCATAATCCAGCTAAGAACAACAGCACCGGAGATCTGAGCTCTGATCTGAAGACATGGCTGAGCGCCGCCGGAGTTAACCCAGAAACATGCATGGAAGGATTCGAAGGCACCAATAGCATTGTCAAAGGATTAGTCTCCGAAGGCATCAACCAATTAACTTCTCAGGTTTATAACCTTCTTTCCATGGTGAAATCGATCTCCAATCAACCGGCCGGAAAAGGGGAGAGCGAGTTTCCTGCCTGGCTCAAAACGGAGGAACAGAGCCTTCTGCAACAAAATGACTTCGCCGCCGATGCGACTGTGGCCGCCGATGGGACAGGGGATTTCACCACCGTGATGGAGGCGGTGCTGGCAGCCCCCGACAAGAGCATGCGACGGTATGTGATATACGTGAAAAAGGGTGTATATTTCGAGAACGTTGAGATCAAGAAGAAGAAATGGAATATCATGATGATCGGAGATGGCATTGACGCCACCGTCATATCCGGTAACCGGAGCTTCATCGACGGCTGGACGACGTTCCGGTCAGCCACCTTCGGTAACCCTTTAACTCTCTCTTCCACTTTGATTCCAAATTAACTGCGGAAAATATCTGCTCCGAAGTGGACCCTTCAACAACTTCGCTTTATGCATACGACTCATTAAGAACAGAACAGACAAACAACCCATGTTTTTTCAAACACAAAGAATCTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACGTTTTTTCATAAATTTCAAACATGTCATGATATTAGTGTAAAAAACACGTGTATTTTTTCTTAAAAATGCTATTAAATTTTCAAAATTAAATAATTTTCTAAATATTAGTACAAAAAATATTAAATGAAAAATATTAATATTTTTTTAAAAAATACTTTCAAGCTTCCAAAATAGTTTTTTAAAAATTATCTTTTTAAATCGGTTTGAAAAATATTTTTTATATGGTGATTTTTTTAATAAAAATTAAAAAATATATACTTAAACGAGTATATAGACGGGACAATGAGTATCACAGTTCAAAAAAATGAAGCCCAAAGATGATAATATTGGTAGGTTTGAGCGGTTACAAATGGTATAAGAGCTAGTCATGAGTCGGTGGGCCAGCAAAGACGCTGGTCCTCGAGGGTTGGATTGTTAGAATCTACCTCGGTATCCAGAATTTATTTGTAAATATTTTTTTAAAAAAAATTATAAATATTAAAAGTATTAATATTACTATAAAAATTCATTAGTATTTTTTAAATATAATATTAATGACAATTGTGTTTTTTTTTTTTTTTTTTTATATATATTTAAGAATATTATTAAAATTATTTTTATTAATTATTTGGTATTTTTTTTTATTTCCAGCGGTTAGTGGAAGAGGGTTCATAGCACGAGACATAACGTTCGAGAACACGGCAGGACCAGAGAAGCATCAAGCCGTGGCACTTAGGTCCGACTCGGACCTATCAGTGTTCTTTAGGTGTCGTATGAGCGGCTACCAAGACACGCTATACACGCACACCATGCGCCAATTCTACCGCGAGTGCCACATCAGCGGCACGGTCGACTTTCTCTTCGGCGACGCAACCGTGGTCTTCCAAAACTGCACCATCCTAGCCAAGAAGGGTCTACCCAACCAGAAAAACACCATCACTGCCCAAGGCCGAAAGGACCCGAATCAGCCCACCGGCTTCTCCATTCAATTCTGCAACATCTCCGCGGATTCCGACCTGCAGCCGTCGGTTAACGCCACGGCAACATACCTCGGGAGGCCATGGAAGGAATACTCAAGAACCATTATCATGCAGTCGTACATTAGCAATGCCATTAGGCCAGAAGGCTGGCTCGAGTGGAATGGCGATTTTGCTTTGAATACTTTGTTTTACGCCGAGTTTATGAATTATGGACCAGGGGCCGGGTTGGCTAAGCGCGTCAACTGGCCGGGTTACCACCGGCTGAACCAAACCAGTGAGGCTACTAACTTCACCGTGTCTCAGTTCATTGAGGGGAACCTTTGGCTGCCATCCACCGGTGTCAAGTTCACCTCAGGATTTGGAGCCAATTAA

mRNA sequence

ATGGCTCCGGCGGCGGATCTTGAACAAACAGAGTGCTTAAAAGTCCCCTCGTCGGAGTTCTCCAACTCTGTTCTCTCCACCATCGGCGTCGTGCGCCAGGTCATGCTCATTTTCTCTCCATTCACTAAATTTCTCGGTGATTCCCGCCTTTCCACCGCCATTTCCGACTGTCTTGATTTGCTGGATTCTTCTTCCGACCAGTTGAGCTGGTCGCTCTCCGCCGCCCAAAATCCGAAGGCTAAGAACAACAGCACCGGAGATCTGAGCTCTGATCTGAAGACATGGCTGAGCGCCGCCGGAGTTAACCCAGAAACATGCATGGAAGGATTCGAAGGCACCAATAGCATTGTCAAAGGATTAGTCTCCGAAGGCATCAACCAATTAACTTCTCAGGTTTATAACCTTCTTTCCATGGTGAAATCGATCTCCAATCAACCGGCCGGAAAAGGGGAGAGCGAGTTTCCTGCCTGGCTCAAAACGGAGGAACAGAGCCTTCTGCAACAAAATGACTTCGCCGCCGATGCGACTGTGGCCGCCGATGGGACAGGGGATTTCACCACCGTGATGGAGGCGGTGCTGGCAGCCCCCGACAAGAGCATGCGACGGTATGTGATATACGTGAAAAAGGGTGTATATTTCGAGAACGTTGAGATCAAGAAGAAGAAATGGAATATCATGATGATCGGAGATGGCATTGACGCCACCGTCATATCCGGTAACCGGAGCTTCATCGACGGCTGGACGACGTTCCGGTCAGCCACCTTCGCGGTTAGTGGAAGAGGGTTCATAGCACGAGACATAACGTTCGAGAACACGGCAGGACCAGAGAAGCATCAAGCCGTGGCACTTAGGTCCGACTCGGACCTATCAGTGTTCTTTAGGTGTCGTATGAGCGGCTACCAAGACACGCTATACACGCACACCATGCGCCAATTCTACCGCGAGTGCCACATCAGCGGCACGGTCGACTTTCTCTTCGGCGACGCAACCGTGGTCTTCCAAAACTGCACCATCCTAGCCAAGAAGGGTCTACCCAACCAGAAAAACACCATCACTGCCCAAGGCCGAAAGGACCCGAATCAGCCCACCGGCTTCTCCATTCAATTCTGCAACATCTCCGCGGATTCCGACCTGCAGCCGTCGGTTAACGCCACGGCAACATACCTCGGGAGGCCATGGAAGGAATACTCAAGAACCATTATCATGCAGTCGTACATTAGCAATGCCATTAGGCCAGAAGGCTGGCTCGAGTGGAATGGCGATTTTGCTTTGAATACTTTGTTTTACGCCGAGTTTATGAATTATGGACCAGGGGCCGGGTTGGCTAAGCGCGTCAACTGGCCGGGTTACCACCGGCTGAACCAAACCAGTGAGGCTACTAACTTCACCGTGTCTCAGTTCATTGAGGGGAACCTTTGGCTGCCATCCACCGGTGTCAAGTTCACCTCAGGATTTGGAGCCAATTAA

Coding sequence (CDS)

ATGGCTCCGGCGGCGGATCTTGAACAAACAGAGTGCTTAAAAGTCCCCTCGTCGGAGTTCTCCAACTCTGTTCTCTCCACCATCGGCGTCGTGCGCCAGGTCATGCTCATTTTCTCTCCATTCACTAAATTTCTCGGTGATTCCCGCCTTTCCACCGCCATTTCCGACTGTCTTGATTTGCTGGATTCTTCTTCCGACCAGTTGAGCTGGTCGCTCTCCGCCGCCCAAAATCCGAAGGCTAAGAACAACAGCACCGGAGATCTGAGCTCTGATCTGAAGACATGGCTGAGCGCCGCCGGAGTTAACCCAGAAACATGCATGGAAGGATTCGAAGGCACCAATAGCATTGTCAAAGGATTAGTCTCCGAAGGCATCAACCAATTAACTTCTCAGGTTTATAACCTTCTTTCCATGGTGAAATCGATCTCCAATCAACCGGCCGGAAAAGGGGAGAGCGAGTTTCCTGCCTGGCTCAAAACGGAGGAACAGAGCCTTCTGCAACAAAATGACTTCGCCGCCGATGCGACTGTGGCCGCCGATGGGACAGGGGATTTCACCACCGTGATGGAGGCGGTGCTGGCAGCCCCCGACAAGAGCATGCGACGGTATGTGATATACGTGAAAAAGGGTGTATATTTCGAGAACGTTGAGATCAAGAAGAAGAAATGGAATATCATGATGATCGGAGATGGCATTGACGCCACCGTCATATCCGGTAACCGGAGCTTCATCGACGGCTGGACGACGTTCCGGTCAGCCACCTTCGCGGTTAGTGGAAGAGGGTTCATAGCACGAGACATAACGTTCGAGAACACGGCAGGACCAGAGAAGCATCAAGCCGTGGCACTTAGGTCCGACTCGGACCTATCAGTGTTCTTTAGGTGTCGTATGAGCGGCTACCAAGACACGCTATACACGCACACCATGCGCCAATTCTACCGCGAGTGCCACATCAGCGGCACGGTCGACTTTCTCTTCGGCGACGCAACCGTGGTCTTCCAAAACTGCACCATCCTAGCCAAGAAGGGTCTACCCAACCAGAAAAACACCATCACTGCCCAAGGCCGAAAGGACCCGAATCAGCCCACCGGCTTCTCCATTCAATTCTGCAACATCTCCGCGGATTCCGACCTGCAGCCGTCGGTTAACGCCACGGCAACATACCTCGGGAGGCCATGGAAGGAATACTCAAGAACCATTATCATGCAGTCGTACATTAGCAATGCCATTAGGCCAGAAGGCTGGCTCGAGTGGAATGGCGATTTTGCTTTGAATACTTTGTTTTACGCCGAGTTTATGAATTATGGACCAGGGGCCGGGTTGGCTAAGCGCGTCAACTGGCCGGGTTACCACCGGCTGAACCAAACCAGTGAGGCTACTAACTTCACCGTGTCTCAGTTCATTGAGGGGAACCTTTGGCTGCCATCCACCGGTGTCAAGTTCACCTCAGGATTTGGAGCCAATTAA

Protein sequence

MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN
BLAST of Carg01161 vs. NCBI nr
Match: XP_022949597.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita moschata])

HSP 1 Score: 978.4 bits (2528), Expect = 8.9e-282
Identity = 485/488 (99.39%), Postives = 486/488 (99.59%), Query Frame = 0

Query: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
           MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 22  MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 81

Query: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
           LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 82  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 141

Query: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
           VSEGINQLTSQVYNLLSMVKSISNQP GKGESEFPAWLKTEEQSLLQQNDFAADATVAAD
Sbjct: 142 VSEGINQLTSQVYNLLSMVKSISNQPVGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 201

Query: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
           GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 202 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 261

Query: 241 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 300
           RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRM GY
Sbjct: 262 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMRGY 321

Query: 301 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 360
           QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN
Sbjct: 322 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 381

Query: 361 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 420
           QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSY+SNAIRPEGWLEWNG
Sbjct: 382 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYVSNAIRPEGWLEWNG 441

Query: 421 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 480
           DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK
Sbjct: 442 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 501

Query: 481 FTSGFGAN 489
           FTSGFGAN
Sbjct: 502 FTSGFGAN 509

BLAST of Carg01161 vs. NCBI nr
Match: XP_023539547.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 978.4 bits (2528), Expect = 8.9e-282
Identity = 486/488 (99.59%), Postives = 487/488 (99.80%), Query Frame = 0

Query: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
           MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 136 MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 195

Query: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
           LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 196 LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 255

Query: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
           VSEGINQLTSQVYNLLSMVKSISNQPAGKGES+FPAWLKTEEQSLLQQNDFAADATVAAD
Sbjct: 256 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESKFPAWLKTEEQSLLQQNDFAADATVAAD 315

Query: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
           GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 316 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 375

Query: 241 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 300
           RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY
Sbjct: 376 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 435

Query: 301 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 360
           QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN
Sbjct: 436 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 495

Query: 361 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 420
           QPTGFSIQFCNISADSDLQP VNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG
Sbjct: 496 QPTGFSIQFCNISADSDLQPLVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 555

Query: 421 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 480
           DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK
Sbjct: 556 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 615

Query: 481 FTSGFGAN 489
           FTSGFGAN
Sbjct: 616 FTSGFGAN 623

BLAST of Carg01161 vs. NCBI nr
Match: XP_022971304.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita maxima])

HSP 1 Score: 969.1 bits (2504), Expect = 5.4e-279
Identity = 482/489 (98.57%), Postives = 486/489 (99.39%), Query Frame = 0

Query: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
           +APAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 110 LAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 169

Query: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
           LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 170 LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 229

Query: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
           VSEGINQLTSQVYNLLSMVKSISNQPAGKGES FPAWLK+EEQSLLQQNDFAADATVAAD
Sbjct: 230 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESGFPAWLKSEEQSLLQQNDFAADATVAAD 289

Query: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
           GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 290 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 349

Query: 241 RSFIDGWTTFRSATFAVSG-RGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSG 300
           RSFIDGWTTFRSATFAVSG RGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSG
Sbjct: 350 RSFIDGWTTFRSATFAVSGRRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSG 409

Query: 301 YQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDP 360
           YQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDP
Sbjct: 410 YQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDP 469

Query: 361 NQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWN 420
           NQPTGFSIQFCNISADSDLQPSVN TATYLGRPWKEYSRTIIMQSY+SNAIRPEGWLEWN
Sbjct: 470 NQPTGFSIQFCNISADSDLQPSVNTTATYLGRPWKEYSRTIIMQSYMSNAIRPEGWLEWN 529

Query: 421 GDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGV 480
           GDFALNTLFYAEFMNYGPGAGLAKR+NWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGV
Sbjct: 530 GDFALNTLFYAEFMNYGPGAGLAKRINWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGV 589

Query: 481 KFTSGFGAN 489
           KFTSGFGAN
Sbjct: 590 KFTSGFGAN 598

BLAST of Carg01161 vs. NCBI nr
Match: XP_008448427.1 (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo])

HSP 1 Score: 875.9 bits (2262), Expect = 6.2e-251
Identity = 431/484 (89.05%), Postives = 459/484 (94.83%), Query Frame = 0

Query: 5   ADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSS 64
           ADL QTECL VPSS+FSNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS
Sbjct: 98  ADLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSS 157

Query: 65  SDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEG 124
           +DQLSWSLSA QNPKAK++STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G
Sbjct: 158 ADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGG 217

Query: 125 INQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGD 184
           INQLTSQ+Y+LLSMVKSI NQP     SEFP+WLK+E+++LLQ ND AADATVAADGTGD
Sbjct: 218 INQLTSQLYDLLSMVKSIPNQP-----SEFPSWLKSEDRNLLQLNDLAADATVAADGTGD 277

Query: 185 FTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFI 244
           FT VM+AVLAAPDKS RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFI
Sbjct: 278 FTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFI 337

Query: 245 DGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTL 304
           DGWTTFRSATFAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTL
Sbjct: 338 DGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTL 397

Query: 305 YTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTG 364
           YTHTMRQFYREC ISGTVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTG
Sbjct: 398 YTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTG 457

Query: 365 FSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFAL 424
           FSIQFCNISADSDL+PSVN TATYLGRPWKEYSRTIIMQSYIS+AIRPEGWLEWNG+FAL
Sbjct: 458 FSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFAL 517

Query: 425 NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 484
           NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTV+QFIEGN+WLPSTGVK+T+G
Sbjct: 518 NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAG 576

Query: 485 FGAN 489
            GAN
Sbjct: 578 LGAN 576

BLAST of Carg01161 vs. NCBI nr
Match: XP_004146188.2 (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus] >KGN55582.1 hypothetical protein Csa_3G000060 [Cucumis sativus])

HSP 1 Score: 874.4 bits (2258), Expect = 1.8e-250
Identity = 430/483 (89.03%), Postives = 457/483 (94.62%), Query Frame = 0

Query: 6   DLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSS 65
           DL QTECL VPSS+FSNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS+
Sbjct: 99  DLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSA 158

Query: 66  DQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGI 125
           DQLSWSLSA QNPKAKN+STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G+
Sbjct: 159 DQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGV 218

Query: 126 NQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGDF 185
           NQLTSQ+Y+LLSMVKSI NQP     SEFP+WLK+E+Q+LLQ ND AADATVAADGTGDF
Sbjct: 219 NQLTSQLYDLLSMVKSIPNQP-----SEFPSWLKSEDQNLLQINDLAADATVAADGTGDF 278

Query: 186 TTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFID 245
           T VM+AVLAAPD S+RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFID
Sbjct: 279 TNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFID 338

Query: 246 GWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLY 305
           GWTTFRSATFAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTLY
Sbjct: 339 GWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLY 398

Query: 306 THTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGF 365
           THTMRQFYREC ISGTVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTGF
Sbjct: 399 THTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGF 458

Query: 366 SIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALN 425
           SIQFCNISADSDL+PSVN TATYLGRPWKEYSRTIIMQSYIS+AIRPEGWLEWN +FALN
Sbjct: 459 SIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALN 518

Query: 426 TLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGF 485
           TLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTV+QFIEGNLWLPSTGVK+T+G 
Sbjct: 519 TLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 576

Query: 486 GAN 489
           GAN
Sbjct: 579 GAN 576

BLAST of Carg01161 vs. TAIR10
Match: AT4G33220.1 (pectin methylesterase 44)

HSP 1 Score: 667.5 bits (1721), Expect = 6.1e-192
Identity = 326/499 (65.33%), Postives = 390/499 (78.16%), Query Frame = 0

Query: 9   QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 68
           Q + LKVP+SEF +S+ + + V+RQV  I S F  F GD RL  A+SDCLDLLD SS++L
Sbjct: 24  QYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGDRRLQNAVSDCLDLLDFSSEEL 83

Query: 69  SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 128
           +WS SA++NPK K N TGD+ SD +TWLSAA  N  TCMEGF+GT+ +VK LV+  ++QL
Sbjct: 84  TWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCMEGFDGTSGLVKSLVAGSLDQL 143

Query: 129 TSQVYNLLSMV------KSISNQ-PAGKGES----------------EFPAWLKTEEQSL 188
            S +  LL +V      K++S   P  KG                  +FP W++ +++ L
Sbjct: 144 YSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLRDTDEDESLQFPDWVRPDDRKL 203

Query: 189 LQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNI 248
           L+ N    D +VA DGTG+FT +M+A+  APD S  R+VIY+KKG+Y ENVEIKKKKWNI
Sbjct: 204 LESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNI 263

Query: 249 MMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRS 308
           +M+GDGID TVISGNRSFIDGWTTFRSATFAVSGRGF+ARDITF+NTAGPEKHQAVALRS
Sbjct: 264 VMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRS 323

Query: 309 DSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLP 368
           DSDLSVFFRC M GYQDTLYTHTMRQFYREC I+GTVDF+FGD TVVFQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLP 383

Query: 369 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSY 428
           NQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +N T TYLGRPWK YSRT+ +++ 
Sbjct: 384 NQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNN 443

Query: 429 ISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVS 485
           +S+ +RPEGWLEWN DFAL+TLFY EFMNYGPG+GL+ RV WPGYH  N + +A NFTVS
Sbjct: 444 MSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVS 503

BLAST of Carg01161 vs. TAIR10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 666.4 bits (1718), Expect = 1.4e-191
Identity = 321/494 (64.98%), Postives = 401/494 (81.17%), Query Frame = 0

Query: 4   AADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDS 63
           + DL Q ECL+VP  EF+ +  + +  + + + I S F K  G SR+S AI DC+DLLDS
Sbjct: 31  STDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDS 90

Query: 64  SSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSE 123
           ++++LSW +SA+Q+P  K+NSTGD+ SDL+TW+SAA  N +TC++GFEGTN I+K +V+ 
Sbjct: 91  AAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKIVAG 150

Query: 124 GINQLTSQVYNLLSMVKSISNQP------------AGKGESEFPAWLKTEEQSLLQ-QND 183
           G++++ + V NLL+MV S  ++P            A  G S+FP+W+K  ++ LLQ  N 
Sbjct: 151 GLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTDNI 210

Query: 184 FAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGD 243
             ADA VAADGTG+FTT+ +AVLAAPD S +RYVI+VK+GVY ENVEIKKKKWNIMM+GD
Sbjct: 211 TVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGD 270

Query: 244 GIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLS 303
           GIDATVI+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAGPEKHQAVA+RSD+DL 
Sbjct: 271 GIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLG 330

Query: 304 VFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNT 363
           VF+RC M GYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQKN+
Sbjct: 331 VFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNS 390

Query: 364 ITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAI 423
           ITAQGRKDPN+PTGF+IQF NI+AD+DL  ++N TATYLGRPWK YSRT+ MQ+Y+S+AI
Sbjct: 391 ITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAI 450

Query: 424 RPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEG 483
            P GWLEWNG+FAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTVSQ I+G
Sbjct: 451 NPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQG 510

Query: 484 NLWLPSTGVKFTSG 485
           NLWLPSTG+ F +G
Sbjct: 511 NLWLPSTGITFIAG 524

BLAST of Carg01161 vs. TAIR10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 436.0 bits (1120), Expect = 3.0e-122
Identity = 228/452 (50.44%), Postives = 299/452 (66.15%), Query Frame = 0

Query: 48  SRLSTAISDCLDLLDSSSDQLSWSL--------SAAQNPKAKNNSTGDLSSDLKTWLSAA 107
           SR   AI DC +L+  S  +L+WS+            +    ++       +LKTWLSAA
Sbjct: 99  SREQVAIEDCKELVGFSVTELAWSMLEMNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAA 158

Query: 108 GVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQP--AGKGESEF--P 167
             N +TC+EGFEGT    + L+   + Q+T  V N+L M   ++  P  A + ES    P
Sbjct: 159 MSNQDTCLEGFEGTERKYEELIKGSLRQVTQLVSNVLDMYTQLNALPFKASRNESVIASP 218

Query: 168 AWLKTEEQSLLQQND---FAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVY 227
            WL   ++SL+ ++D      +  VA DG G + T+ EA+  AP+ S +RYVIYVKKGVY
Sbjct: 219 EWLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVY 278

Query: 228 FENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 287
            EN+++KKKK NIM++GDGI  T+I+G+R+F+ G TTFR+AT AVSGRGFIA+DITF NT
Sbjct: 279 KENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNT 338

Query: 288 AGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVV 347
           AGP+  QAVALR DSD S F+RC + GYQDTLY H++RQFYR+C I GT+DF+FG+   V
Sbjct: 339 AGPQNRQAVALRVDSDQSAFYRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAV 398

Query: 348 FQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRP 407
            QNC I  +  LP QK TITAQGRK PNQ TGF IQ   + A    QP      TYLGRP
Sbjct: 399 LQNCKIYTRVPLPLQKVTITAQGRKSPNQNTGFVIQNSYVLA---TQP------TYLGRP 458

Query: 408 WKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 467
           WK YSRT+ M +Y+S  ++P GWLEW G+FAL+TL+Y E+ N GPG   + RV WPGYH 
Sbjct: 459 WKLYSRTVYMNTYMSQLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHI 518

Query: 468 LNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 485
           +++ + A +FTV  FI+G  WLP+TGV FT+G
Sbjct: 519 MDKRT-ALSFTVGSFIDGRRWLPATGVTFTAG 540

BLAST of Carg01161 vs. TAIR10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 427.6 bits (1098), Expect = 1.1e-119
Identity = 225/454 (49.56%), Postives = 296/454 (65.20%), Query Frame = 0

Query: 49  RLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCME 108
           R  +A   C++LLD S D LS +LS+  +  AK         D+ TWLSAA  N +TC E
Sbjct: 149 RARSAYDSCVELLDDSVDALSRALSSVVSSSAK-------PQDVTTWLSAALTNHDTCTE 208

Query: 109 GFEGT-NSIVKGLVSEGINQLTSQVYNLLSMVKS-----------ISNQ---PAGKGESE 168
           GF+G  +  VK  ++  +  L+  V N L++  +           I N+      + E +
Sbjct: 209 GFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEK 268

Query: 169 FPAWLKTEEQSLLQQ--NDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGV 228
           FP W++ +E+ +L+   +   AD  V+ DG G   T+ EA+  AP  S RR +IYVK G 
Sbjct: 269 FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGR 328

Query: 229 YFE-NVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 288
           Y E N+++ +KK N+M +GDG   TVISG +S  D  TTF +A+FA +G GFIARDITFE
Sbjct: 329 YEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFE 388

Query: 289 NTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDAT 348
           N AGP KHQAVALR  +D +V +RC + GYQDTLY H+ RQF+REC I GTVDF+FG+A 
Sbjct: 389 NWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAA 448

Query: 349 VVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLG 408
           VV QNC+I A+K +  QKNTITAQ RKDPNQ TG SI    + A SDLQ +  +T TYLG
Sbjct: 449 VVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLG 508

Query: 409 RPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGY 468
           RPWK +SRT+ M SYI   +   GWLEWN  FAL+TL+Y E++N GPG+GL +RV+WPGY
Sbjct: 509 RPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGY 568

Query: 469 HRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 485
             +N T+EA  FTV++FI G+ WLPSTGV F +G
Sbjct: 569 RVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAG 595

BLAST of Carg01161 vs. TAIR10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F)

HSP 1 Score: 424.5 bits (1090), Expect = 9.0e-119
Identity = 231/455 (50.77%), Postives = 295/455 (64.84%), Query Frame = 0

Query: 49  RLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLS-SDLKTWLSAAGVNPETCM 108
           R+ +A   CL+LLD S D L+ +LS+          +GD S SD+ TWLS+A  N +TC 
Sbjct: 136 RVRSAYDSCLELLDDSVDALTRALSSVV------VVSGDESHSDVMTWLSSAMTNHDTCT 195

Query: 109 EGF---EGTNSIVKGLVSEGINQLTSQVYNLLSM----VKSISNQPA-------GKGES- 168
           +GF   EG    VK  V   +  L+  V N L++    VK +S  P        G  E+ 
Sbjct: 196 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETE 255

Query: 169 EFPAWLKTEEQSLL--QQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKG 228
           E P WLK E++ LL    +   AD TV+ DG+G F T+ EA+  AP+ S RR+VIYVK G
Sbjct: 256 ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAG 315

Query: 229 VY-FENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITF 288
            Y  EN+++ +KK N+M IGDG   TVI+G +S  D  TTF +ATFA +G GFI RD+TF
Sbjct: 316 RYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTF 375

Query: 289 ENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDA 348
           EN AGP KHQAVALR   D +V +RC + GYQD LY H+ RQF+REC I GTVDF+FG+A
Sbjct: 376 ENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNA 435

Query: 349 TVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYL 408
            V+ Q+C I A+K +  QK TITAQ RKDPNQ TG SI  C + A  DL+ S  +  TYL
Sbjct: 436 AVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYL 495

Query: 409 GRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPG 468
           GRPWK YSR + M S + + I P GWLEWNG FAL++L+Y E+MN G G+G+ +RV WPG
Sbjct: 496 GRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPG 555

Query: 469 YHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 485
           YH +  T EA+ FTV+QFI G+ WLPSTGV F SG
Sbjct: 556 YHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSG 584

BLAST of Carg01161 vs. Swiss-Prot
Match: sp|Q43062|PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.0e-208
Identity = 352/479 (73.49%), Postives = 408/479 (85.18%), Query Frame = 0

Query: 9   QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 68
           QT+CLKV ++EF+ S+  TI  V+QV  I S F    GD RL+ AISDCLDLLD S+D+L
Sbjct: 45  QTQCLKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADEL 104

Query: 69  SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 128
           +WSLSA+QN K KNNSTG LSSDL+TWLSAA VN +TC  GFEGTNSIV+GL+S G+ Q+
Sbjct: 105 NWSLSASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQV 164

Query: 129 TSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTV 188
           TS V  LL+ V   SNQ    G  + P+W+KT+++ LLQ +  + DA VA DGTG+FT V
Sbjct: 165 TSLVQELLTQVHPNSNQQGPNG--QIPSWVKTKDRKLLQADGVSVDAIVAQDGTGNFTNV 224

Query: 189 MEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWT 248
            +AVLAAPD SMRRYVIY+K+G Y ENVEIKKKKWN+MMIGDG+DAT+ISGNRSF+DGWT
Sbjct: 225 TDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWT 284

Query: 249 TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHT 308
           TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVF+RC + GYQDTLYTHT
Sbjct: 285 TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHT 344

Query: 309 MRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQ 368
           MRQFYR+C ISGTVDF+FGDATVVFQNC ILAKKGLPNQKN+ITAQGRKDPN+PTG SIQ
Sbjct: 345 MRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQ 404

Query: 369 FCNISADSDLQ-PSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTL 428
           FCNI+ADSDL+  SVN+T TYLGRPWK YSRT+IMQS++SN IRPEGWLEWNGDFALN+L
Sbjct: 405 FCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSL 464

Query: 429 FYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGFG 487
           FY E+MNYGPGAGL  RV WPGY   N++++A N+TV+QFIEGNLWLPSTGVK+T+ FG
Sbjct: 465 FYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFG 521

BLAST of Carg01161 vs. Swiss-Prot
Match: sp|Q9SMY7|PME44_ARATH (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 667.5 bits (1721), Expect = 1.1e-190
Identity = 326/499 (65.33%), Postives = 390/499 (78.16%), Query Frame = 0

Query: 9   QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 68
           Q + LKVP+SEF +S+ + + V+RQV  I S F  F GD RL  A+SDCLDLLD SS++L
Sbjct: 24  QYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGDRRLQNAVSDCLDLLDFSSEEL 83

Query: 69  SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 128
           +WS SA++NPK K N TGD+ SD +TWLSAA  N  TCMEGF+GT+ +VK LV+  ++QL
Sbjct: 84  TWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCMEGFDGTSGLVKSLVAGSLDQL 143

Query: 129 TSQVYNLLSMV------KSISNQ-PAGKGES----------------EFPAWLKTEEQSL 188
            S +  LL +V      K++S   P  KG                  +FP W++ +++ L
Sbjct: 144 YSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLRDTDEDESLQFPDWVRPDDRKL 203

Query: 189 LQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNI 248
           L+ N    D +VA DGTG+FT +M+A+  APD S  R+VIY+KKG+Y ENVEIKKKKWNI
Sbjct: 204 LESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNI 263

Query: 249 MMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRS 308
           +M+GDGID TVISGNRSFIDGWTTFRSATFAVSGRGF+ARDITF+NTAGPEKHQAVALRS
Sbjct: 264 VMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRS 323

Query: 309 DSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLP 368
           DSDLSVFFRC M GYQDTLYTHTMRQFYREC I+GTVDF+FGD TVVFQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLP 383

Query: 369 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSY 428
           NQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +N T TYLGRPWK YSRT+ +++ 
Sbjct: 384 NQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNN 443

Query: 429 ISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVS 485
           +S+ +RPEGWLEWN DFAL+TLFY EFMNYGPG+GL+ RV WPGYH  N + +A NFTVS
Sbjct: 444 MSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVS 503

BLAST of Carg01161 vs. Swiss-Prot
Match: sp|Q9LXK7|PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 2.5e-190
Identity = 321/494 (64.98%), Postives = 401/494 (81.17%), Query Frame = 0

Query: 4   AADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDS 63
           + DL Q ECL+VP  EF+ +  + +  + + + I S F K  G SR+S AI DC+DLLDS
Sbjct: 31  STDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDS 90

Query: 64  SSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSE 123
           ++++LSW +SA+Q+P  K+NSTGD+ SDL+TW+SAA  N +TC++GFEGTN I+K +V+ 
Sbjct: 91  AAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKIVAG 150

Query: 124 GINQLTSQVYNLLSMVKSISNQP------------AGKGESEFPAWLKTEEQSLLQ-QND 183
           G++++ + V NLL+MV S  ++P            A  G S+FP+W+K  ++ LLQ  N 
Sbjct: 151 GLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTDNI 210

Query: 184 FAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGD 243
             ADA VAADGTG+FTT+ +AVLAAPD S +RYVI+VK+GVY ENVEIKKKKWNIMM+GD
Sbjct: 211 TVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGD 270

Query: 244 GIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLS 303
           GIDATVI+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAGPEKHQAVA+RSD+DL 
Sbjct: 271 GIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLG 330

Query: 304 VFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNT 363
           VF+RC M GYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQKN+
Sbjct: 331 VFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNS 390

Query: 364 ITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAI 423
           ITAQGRKDPN+PTGF+IQF NI+AD+DL  ++N TATYLGRPWK YSRT+ MQ+Y+S+AI
Sbjct: 391 ITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAI 450

Query: 424 RPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEG 483
            P GWLEWNG+FAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTVSQ I+G
Sbjct: 451 NPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQG 510

Query: 484 NLWLPSTGVKFTSG 485
           NLWLPSTG+ F +G
Sbjct: 511 NLWLPSTGITFIAG 524

BLAST of Carg01161 vs. Swiss-Prot
Match: sp|Q96575|PME22_SOLLC (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 8.9e-124
Identity = 227/489 (46.42%), Postives = 319/489 (65.24%), Query Frame = 0

Query: 5   ADLEQTECLKVPSSEFSNSVLSTIGVVRQV---MLIFSPFTKFLGDSRLSTAISDCLDLL 64
           +DL   E +   S   S  +   +  V Q+   + + S     + D R   A++DCL+LL
Sbjct: 68  SDLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQEGALTDCLELL 127

Query: 65  DSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEG-TNSIVKGL 124
           D S D +S S++A       +  T    ++ ++WLS    N  TC++  +  T +++ G 
Sbjct: 128 DQSVDLVSDSIAAI------DKRTHSEHANAQSWLSGVLTNHVTCLDELDSFTKAMING- 187

Query: 125 VSEGINQLTSQVYNLLSMVKSI---SNQPAGKGESEFPAWLKTEEQSLLQQN--DFAADA 184
               +++L S+    L+M+ S+   ++     G  + P+W+ + ++ L++ +  D  A+A
Sbjct: 188 --TNLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGANA 247

Query: 185 TVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDAT 244
            VA DGTG + T+ EAV AAPDKS  RYVIYVK+G+Y ENVE+  +K  +M++GDG+ AT
Sbjct: 248 VVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMHAT 307

Query: 245 VISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRC 304
           +I+GN + +DG TTF SAT A  G+GFI +DI  +NTAGP KHQAVALR  +D SV  RC
Sbjct: 308 IITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVINRC 367

Query: 305 RMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQG 364
           R+  YQDTLY H+ RQFYR+ +++GT+DF+FG+A VVFQ C ++A+K    Q+N +TAQG
Sbjct: 368 RIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQQNMVTAQG 427

Query: 365 RKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGW 424
           R DPNQ TG SIQFCNI A SDL+P +    TYLGRPWK+YSRT++M+SY+   I P GW
Sbjct: 428 RTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPAGW 487

Query: 425 LEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLP 484
            EW+GDFAL TL+Y EFMN GPGAG +KRV WPGYH +   +EA  FTV++ I+G  WL 
Sbjct: 488 AEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSWLR 547

BLAST of Carg01161 vs. Swiss-Prot
Match: sp|P14280|PME1_SOLLC (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 438.3 bits (1126), Expect = 1.1e-121
Identity = 220/446 (49.33%), Postives = 301/446 (67.49%), Query Frame = 0

Query: 45  LGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPE 104
           + D R   A++DCL+LLD S D  S S++A        +      ++ ++WLS    N  
Sbjct: 107 INDIRQHGALTDCLELLDQSVDFASDSIAAIDKRSRSEH------ANAQSWLSGVLTNHV 166

Query: 105 TCMEGFEG-TNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGES---EFPAWLKT 164
           TC++  +  T +++ G     + +L S+    L+M+ S++ Q      +   + P+W+ +
Sbjct: 167 TCLDELDSFTKAMING---TNLEELISRAKVALAMLASLTTQDEDVFMTVLGKMPSWVSS 226

Query: 165 EEQSLLQQN--DFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEI 224
            ++ L++ +  D  A+A VA DGTGD+ T+ EAV AAPDKS  RYVIYVK+G Y ENVE+
Sbjct: 227 MDRKLMESSGKDIIANAVVAQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEV 286

Query: 225 KKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKH 284
              K N+M++GDG+ AT I+G+ + +DG TTFRSAT A  G+GFI +DI  +NTAGP K 
Sbjct: 287 ASNKMNLMIVGDGMYATTITGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKD 346

Query: 285 QAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTI 344
           QAVALR  +D+SV  RCR+  YQDTLY H+ RQFYR+ +++GTVDF+FG+A VVFQ C +
Sbjct: 347 QAVALRVGADMSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQL 406

Query: 345 LAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSR 404
           +A+K    Q+N +TAQGR DPNQ TG SIQFCNI A SDL+P +    TYLGRPWKEYSR
Sbjct: 407 VARKPGKYQQNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSR 466

Query: 405 TIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSE 464
           T++M+SY+   I P GW EW+GDFAL TL+Y EFMN GPGAG +KRV WPGYH +   ++
Sbjct: 467 TVVMESYLGGLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAK 526

Query: 465 ATNFTVSQFIEGNLWLPSTGVKFTSG 485
           A  FTV++ I+G  WL STGV +  G
Sbjct: 527 AMPFTVAKLIQGGSWLRSTGVAYVDG 543

BLAST of Carg01161 vs. TrEMBL
Match: tr|A0A1S3BKA0|A0A1S3BKA0_CUCME (pectinesterase/pectinesterase inhibitor PPE8B-like OS=Cucumis melo OX=3656 GN=LOC103490630 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 4.1e-251
Identity = 431/484 (89.05%), Postives = 459/484 (94.83%), Query Frame = 0

Query: 5   ADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSS 64
           ADL QTECL VPSS+FSNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS
Sbjct: 98  ADLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSS 157

Query: 65  SDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEG 124
           +DQLSWSLSA QNPKAK++STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G
Sbjct: 158 ADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGG 217

Query: 125 INQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGD 184
           INQLTSQ+Y+LLSMVKSI NQP     SEFP+WLK+E+++LLQ ND AADATVAADGTGD
Sbjct: 218 INQLTSQLYDLLSMVKSIPNQP-----SEFPSWLKSEDRNLLQLNDLAADATVAADGTGD 277

Query: 185 FTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFI 244
           FT VM+AVLAAPDKS RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFI
Sbjct: 278 FTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFI 337

Query: 245 DGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTL 304
           DGWTTFRSATFAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTL
Sbjct: 338 DGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTL 397

Query: 305 YTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTG 364
           YTHTMRQFYREC ISGTVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTG
Sbjct: 398 YTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTG 457

Query: 365 FSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFAL 424
           FSIQFCNISADSDL+PSVN TATYLGRPWKEYSRTIIMQSYIS+AIRPEGWLEWNG+FAL
Sbjct: 458 FSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFAL 517

Query: 425 NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 484
           NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTV+QFIEGN+WLPSTGVK+T+G
Sbjct: 518 NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAG 576

Query: 485 FGAN 489
            GAN
Sbjct: 578 LGAN 576

BLAST of Carg01161 vs. TrEMBL
Match: tr|A0A0A0L4S0|A0A0A0L4S0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G000060 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 1.2e-250
Identity = 430/483 (89.03%), Postives = 457/483 (94.62%), Query Frame = 0

Query: 6   DLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSS 65
           DL QTECL VPSS+FSNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS+
Sbjct: 99  DLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSA 158

Query: 66  DQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGI 125
           DQLSWSLSA QNPKAKN+STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G+
Sbjct: 159 DQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGV 218

Query: 126 NQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGDF 185
           NQLTSQ+Y+LLSMVKSI NQP     SEFP+WLK+E+Q+LLQ ND AADATVAADGTGDF
Sbjct: 219 NQLTSQLYDLLSMVKSIPNQP-----SEFPSWLKSEDQNLLQINDLAADATVAADGTGDF 278

Query: 186 TTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFID 245
           T VM+AVLAAPD S+RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFID
Sbjct: 279 TNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFID 338

Query: 246 GWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLY 305
           GWTTFRSATFAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTLY
Sbjct: 339 GWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLY 398

Query: 306 THTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGF 365
           THTMRQFYREC ISGTVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTGF
Sbjct: 399 THTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGF 458

Query: 366 SIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALN 425
           SIQFCNISADSDL+PSVN TATYLGRPWKEYSRTIIMQSYIS+AIRPEGWLEWN +FALN
Sbjct: 459 SIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALN 518

Query: 426 TLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGF 485
           TLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTV+QFIEGNLWLPSTGVK+T+G 
Sbjct: 519 TLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 576

Query: 486 GAN 489
           GAN
Sbjct: 579 GAN 576

BLAST of Carg01161 vs. TrEMBL
Match: tr|A0A2N9H526|A0A2N9H526_FAGSY (Pectinesterase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34536 PE=3 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 5.3e-214
Identity = 363/484 (75.00%), Postives = 417/484 (86.16%), Query Frame = 0

Query: 6   DLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSS 65
           DL Q+ECLKVPSSEF NSV +TI  V+ V  I SPF     D R+S AISDCLDLLD S+
Sbjct: 24  DLLQSECLKVPSSEFLNSVKNTIDAVQNVASIISPFANVFQDLRISNAISDCLDLLDFSA 83

Query: 66  DQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGI 125
           D+LSWSLSA QNPK KNNSTGDLSSDL+TWLSAA VN +TC+EGF+GTNSIV+GLV+ G+
Sbjct: 84  DELSWSLSATQNPKGKNNSTGDLSSDLRTWLSAALVNQDTCIEGFDGTNSIVQGLVTGGL 143

Query: 126 NQLTSQVYNLLSMVKSISNQPAGKGES--EFPAWLKTEEQSLLQQNDFAADATVAADGTG 185
           NQ+TS V  LL+MV  +S+ P  + ES  +FP+W+K E++ L+Q N    DA VAADG+G
Sbjct: 144 NQVTSLVQQLLNMVHPVSDNPGRRLESNDQFPSWMKLEDRKLVQANGVTVDAIVAADGSG 203

Query: 186 DFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSF 245
           +FT VM+A+LAAPD SMRRYVIY+KKGVY E VEIKKKKWN+MMIGDG++AT+ISGNR++
Sbjct: 204 NFTKVMDAILAAPDFSMRRYVIYIKKGVYNEYVEIKKKKWNLMMIGDGMNATIISGNRNY 263

Query: 246 IDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDT 305
           +DGWTTF +ATFAVSGRGFIARDITFEN+AGPEKHQAVALRSDSDLSVFFRC + GYQDT
Sbjct: 264 VDGWTTFHTATFAVSGRGFIARDITFENSAGPEKHQAVALRSDSDLSVFFRCGIRGYQDT 323

Query: 306 LYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPT 365
           LYTHTMRQFYREC ISGT+DF+FGD +VVFQNC ILA+ GLPNQKNTITAQGRKDPNQPT
Sbjct: 324 LYTHTMRQFYRECRISGTIDFIFGDGSVVFQNCQILARMGLPNQKNTITAQGRKDPNQPT 383

Query: 366 GFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFA 425
           GFSIQFCNIS DSDL  SVN+T+TYLGRPWK YSRTIIMQSYISNA RPEGWLEWNG   
Sbjct: 384 GFSIQFCNISVDSDLATSVNSTSTYLGRPWKPYSRTIIMQSYISNATRPEGWLEWNGSLY 443

Query: 426 LNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTS 485
           L+TLFY E+MNYGPG+GL  RV WPGYH LNQ+S+ TNFTV+QFIEGNLWLPSTGVK+T+
Sbjct: 444 LDTLFYGEYMNYGPGSGLGNRVKWPGYHALNQSSQVTNFTVAQFIEGNLWLPSTGVKYTA 503

Query: 486 GFGA 488
           G G+
Sbjct: 504 GLGS 507

BLAST of Carg01161 vs. TrEMBL
Match: tr|A0A2P4HW96|A0A2P4HW96_QUESU (Pectinesterase OS=Quercus suber OX=58331 GN=CFP56_61012 PE=3 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 8.7e-209
Identity = 350/485 (72.16%), Postives = 408/485 (84.12%), Query Frame = 0

Query: 7   LEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSD 66
           L+ +ECLKVP+SEF +SV +TI  V+ V+ I SPF K  GD R+S AISDCLDLLD S+D
Sbjct: 22  LQYSECLKVPTSEFLSSVKTTIDAVQSVVSIISPFAKLFGDLRVSNAISDCLDLLDFSAD 81

Query: 67  QLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGIN 126
           +L+WSLSA QNPK   N TGDLSSDL+TWLSAA VN +TC+EGFEGTNSIV GL+  G+N
Sbjct: 82  ELAWSLSATQNPKGNQNGTGDLSSDLRTWLSAALVNQDTCIEGFEGTNSIVAGLIDGGLN 141

Query: 127 QLTSQVYNLLSMVKSISNQP-----AGKGESEFPAWLKTEEQSLLQQNDFAADATVAADG 186
           Q+TS V  LL MV  + N P     +G    +FP+W+K+E++ LLQ N F  D  VAADG
Sbjct: 142 QVTSLVQQLLDMVHPVLNNPRGRRLSGNANEQFPSWVKSEDRKLLQANGFTVDVIVAADG 201

Query: 187 TGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNR 246
           +G++T +M+A+  APD SMRRYVIY+KKGVY E VEIKKKKWN+MMIGDG+DAT+I+GN 
Sbjct: 202 SGNYTKMMDAIKDAPDYSMRRYVIYIKKGVYNETVEIKKKKWNLMMIGDGMDATIITGNH 261

Query: 247 SFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQ 306
           +++DGWTTF SATFAVSG+GFIARD+TFENTAGPEKHQAVALRSDSDLSVFFRC + GYQ
Sbjct: 262 NYVDGWTTFHSATFAVSGKGFIARDMTFENTAGPEKHQAVALRSDSDLSVFFRCGIRGYQ 321

Query: 307 DTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQ 366
           DTLYTHTMRQFYREC ISGT+DF+FGD +VVFQNC IL +KGLPNQKNTITAQGRKDP++
Sbjct: 322 DTLYTHTMRQFYRECRISGTIDFIFGDGSVVFQNCDILVRKGLPNQKNTITAQGRKDPSE 381

Query: 367 PTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGD 426
           PTGFSIQFCNISADSDL PSVN+T TYLGRPWK YSRTIIMQSYISNA RPEGWLEWNG 
Sbjct: 382 PTGFSIQFCNISADSDLVPSVNSTYTYLGRPWKAYSRTIIMQSYISNATRPEGWLEWNGT 441

Query: 427 FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKF 486
             L+TL+Y E+MN GPGAGL+ RV WPGYH LNQ+S+  NFTV+QFIEGNLWLPSTGVK+
Sbjct: 442 LHLDTLYYGEYMNNGPGAGLSNRVKWPGYHALNQSSQVNNFTVAQFIEGNLWLPSTGVKY 501

BLAST of Carg01161 vs. TrEMBL
Match: tr|M5WFH0|M5WFH0_PRUPE (Pectinesterase OS=Prunus persica OX=3760 GN=PRUPE_7G190300 PE=3 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 4.0e-206
Identity = 352/479 (73.49%), Postives = 408/479 (85.18%), Query Frame = 0

Query: 9   QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 68
           QT+CLKV ++EF+ S+  TI  V+QV  I S F    GD RL+ AISDCLDLLD S+D+L
Sbjct: 40  QTQCLKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADEL 99

Query: 69  SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 128
           +WSLSA+QN K KNNSTG LSSDL+TWLSAA VN +TC  GFEGTNSIV+GL+S G+ Q+
Sbjct: 100 NWSLSASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQV 159

Query: 129 TSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTV 188
           TS V  LL+ V   SNQ    G  + P+W+KT+++ LLQ +  + DA VA DGTG+FT V
Sbjct: 160 TSLVQELLTQVHPNSNQQGPNG--QIPSWVKTKDRKLLQADGVSVDAIVAQDGTGNFTNV 219

Query: 189 MEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWT 248
            +AVLAAPD SMRRYVIY+K+G Y ENVEIKKKKWN+MMIGDG+DAT+ISGNRSF+DGWT
Sbjct: 220 TDAVLAAPDYSMRRYVIYIKRGTYRENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWT 279

Query: 249 TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHT 308
           TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVF+RC + GYQDTLYTHT
Sbjct: 280 TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHT 339

Query: 309 MRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQ 368
           MRQFYR+C ISGTVDF+FGDATVVFQNC ILAKKGLPNQKN+ITAQGRKDPN+PTG SIQ
Sbjct: 340 MRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQ 399

Query: 369 FCNISADSDLQ-PSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTL 428
           FCNI+ADSDL+  SVN+T TYLGRPWK YSRT+IMQS++SN IRPEGWLEWNGDFALN+L
Sbjct: 400 FCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSL 459

Query: 429 FYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGFG 487
           FY E+MNYGPGAGL  RV WPGY   N++++A N+TV+QFIEGNLWLPSTGVK+T+ FG
Sbjct: 460 FYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFG 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022949597.18.9e-28299.39pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita moschata][more]
XP_023539547.18.9e-28299.59pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita pepo subsp. pepo][more]
XP_022971304.15.4e-27998.57pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita maxima][more]
XP_008448427.16.2e-25189.05PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo][more]
XP_004146188.21.8e-25089.03PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus] ... [more]
Match NameE-valueIdentityDescription
AT4G33220.16.1e-19265.33pectin methylesterase 44[more]
AT3G43270.11.4e-19164.98Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G05620.13.0e-12250.44Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G49220.11.1e-11949.56Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G53370.19.0e-11950.77pectin methylesterase PCR fragment F[more]
Match NameE-valueIdentityDescription
sp|Q43062|PME_PRUPE2.0e-20873.49Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=... [more]
sp|Q9SMY7|PME44_ARATH1.1e-19065.33Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3... [more]
sp|Q9LXK7|PME32_ARATH2.5e-19064.98Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3... [more]
sp|Q96575|PME22_SOLLC8.9e-12446.42Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1[more]
sp|P14280|PME1_SOLLC1.1e-12149.33Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BKA0|A0A1S3BKA0_CUCME4.1e-25189.05pectinesterase/pectinesterase inhibitor PPE8B-like OS=Cucumis melo OX=3656 GN=LO... [more]
tr|A0A0A0L4S0|A0A0A0L4S0_CUCSA1.2e-25089.03Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G000060 PE=3 SV=1[more]
tr|A0A2N9H526|A0A2N9H526_FAGSY5.3e-21475.00Pectinesterase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34536 PE=3 SV=1[more]
tr|A0A2P4HW96|A0A2P4HW96_QUESU8.7e-20972.16Pectinesterase OS=Quercus suber OX=58331 GN=CFP56_61012 PE=3 SV=1[more]
tr|M5WFH0|M5WFH0_PRUPE4.0e-20673.49Pectinesterase OS=Prunus persica OX=3760 GN=PRUPE_7G190300 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
IPR035513Invertase/methylesterase_inhib
IPR012334Pectin_lyas_fold
IPR006501Pectinesterase_inhib_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01161-RACarg01161-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 3..142
e-value: 1.9E-10
score: 50.7
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 52..137
e-value: 1.4E-13
score: 51.3
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 171..487
e-value: 2.2E-135
score: 453.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3DG3DSA:1.20.140.40coord: 37..145
e-value: 1.3E-6
score: 30.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILYSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 33..143
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 174..471
e-value: 3.8E-151
score: 501.8
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 5..485
NoneNo IPR availablePANTHERPTHR31707:SF10PECTINESTERASE/PECTINESTERASE INHIBITOR 32-RELATEDcoord: 5..485
NoneNo IPR availableCDDcd15799PMEI-like_4coord: 1..143
e-value: 3.91224E-38
score: 136.001
IPR011050Pectin lyase fold/virulence factorSUPERFAMILYSSF51126Pectin lyase-likecoord: 172..484