Cp4.1LG08g12640 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG08g12640
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG08 : 9206612 .. 9207860 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCCCACAACGTGATTGTCAAAGCGACCGTGCCATTCAAATCAACGCGCCATCTCCCCAACCTCCGCCTCCGCCCCTTCTTAAATTCCCTCACCTTCCCACCCAAATCCTTCATTTATTCCGGCTCCCCTGTTTCCTCCTCCCCTGTTACCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCCCTGTCTCCTCCCCTAACACAAACCACAAGCAAAGCCACCTCAATAATCCCATCTTAATGGCCAAACTAACCAAAACCCACCTCAAATTTTGGCTGTTTATGCTGCTGATCCTCTTCCCCATGGCTCCGGCGGCGGATCTTGAACAAACAGAGTGCTTAAAAGTCCCCTCCTCGGAGTTCTCCAACTCTGTTCTCTCCACCATCGGCGTCGTGCGCCAGGTCATGCTCATTTTCTCTCCGTTCACTAAATTTCTCGGGGATTCCCGCCTTTCCACCGCCATTTCCGACTGTCTCGATTTGCTGGATTCTTCTTCCGACCAGTTGAGCTGGTCGCTCTCCGCCGCCCAAAACCCAAAGGGTAAACGGTCTTTCTCCCCAAAACATAATAAATTTCGTGTTTGCATGTTAATCCACATCTGTTTTCGTTTTGCATAATCCAGCTAAGAACAACAGCACCGGAGATCTGAGCTCTGATCTGAAAACATGGCTGAGCGCCGCCGGAGTTAACCCAGAAACATGCATGGAAGGATTCGAAGGCACCAATAGCATTGTCAAAGGATTAGTTTCCGAAGGCATCAACCAATTAACTTCACAGGTTTATAACCTTCTTTCCATGGTGAAATCGATCTCCAATCAGCCGGCCGGAAAAGGGGAGAGCAAGTTTCCCGCCTGGCTCAAAACGGAGGAACAGAGCCTGCTGCAACAAAATGACTTCGCCGCCGATGCGACTGTGGCCGCCGATGGGACAGGGGATTTCACCACCGTAATGGAGGCGGTGCTGGCAGCCCCCGACAAGAGCATGCGGCGGTATGTGATATACGTGAAAAAGGGTGTGTATTTCGAGAACGTCGAGATCAAGAAGAAGAAATGGAATATCATGATGATCGGAGATGGCATTGACGCCACCGTCATATCCGGTAACCGGAGCTTCATCGACGGCTGGACGACGTTCCGATCAGCCACCTTCGGTAACCCTTTAACTCTCTCTTCCACTTTGATTTGA

mRNA sequence

CTTCCCCACAACGTGATTGTCAAAGCGACCGTGCCATTCAAATCAACGCGCCATCTCCCCAACCTCCGCCTCCGCCCCTTCTTAAATTCCCTCACCTTCCCACCCAAATCCTTCATTTATTCCGGCTCCCCTGTTTCCTCCTCCCCTGTTACCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCCCTGTCTCCTCCCCTAACACAAACCACAAGCAAAGCCACCTCAATAATCCCATCTTAATGGCCAAACTAACCAAAACCCACCTCAAATTTTGGCTGTTTATGCTGCTGATCCTCTTCCCCATGGCTCCGGCGGCGGATCTTGAACAAACAGAGTGCTTAAAAGTCCCCTCCTCGGAGTTCTCCAACTCTGTTCTCTCCACCATCGGCGTCGTGCGCCAGGTCATGCTCATTTTCTCTCCGTTCACTAAATTTCTCGGGGATTCCCGCCTTTCCACCGCCATTTCCGACTGTCTCGATTTGCTGGATTCTTCTTCCGACCAGTTGAGCTGGTCGCTCTCCGCCGCCCAAAACCCAAAGGCTAAGAACAACAGCACCGGAGATCTGAGCTCTGATCTGAAAACATGGCTGAGCGCCGCCGGAGTTAACCCAGAAACATGCATGGAAGGATTCGAAGGCACCAATAGCATTGTCAAAGGATTAGTTTCCGAAGGCATCAACCAATTAACTTCACAGGTTTATAACCTTCTTTCCATGGTGAAATCGATCTCCAATCAGCCGGCCGGAAAAGGGGAGAGCAAGTTTCCCGCCTGGCTCAAAACGGAGGAACAGAGCCTGCTGCAACAAAATGACTTCGCCGCCGATGCGACTGTGGCCGCCGATGGGACAGGGGATTTCACCACCGTAATGGAGGCGGTGCTGGCAGCCCCCGACAAGAGCATGCGGCGGTATGTGATATACGTGAAAAAGGGTGTGTATTTCGAGAACGTCGAGATCAAGAAGAAGAAATGGAATATCATGATGATCGGAGATGGCATTGACGCCACCGTCATATCCGGTAACCGGAGCTTCATCGACGGCTGGACGACGTTCCGATCAGCCACCTTCGGTAACCCTTTAACTCTCTCTTCCACTTTGATTTGA

Coding sequence (CDS)

CTTCCCCACAACGTGATTGTCAAAGCGACCGTGCCATTCAAATCAACGCGCCATCTCCCCAACCTCCGCCTCCGCCCCTTCTTAAATTCCCTCACCTTCCCACCCAAATCCTTCATTTATTCCGGCTCCCCTGTTTCCTCCTCCCCTGTTACCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCTCCCCTGTTTCCTCCCCTGTCTCCTCCCCTAACACAAACCACAAGCAAAGCCACCTCAATAATCCCATCTTAATGGCCAAACTAACCAAAACCCACCTCAAATTTTGGCTGTTTATGCTGCTGATCCTCTTCCCCATGGCTCCGGCGGCGGATCTTGAACAAACAGAGTGCTTAAAAGTCCCCTCCTCGGAGTTCTCCAACTCTGTTCTCTCCACCATCGGCGTCGTGCGCCAGGTCATGCTCATTTTCTCTCCGTTCACTAAATTTCTCGGGGATTCCCGCCTTTCCACCGCCATTTCCGACTGTCTCGATTTGCTGGATTCTTCTTCCGACCAGTTGAGCTGGTCGCTCTCCGCCGCCCAAAACCCAAAGGCTAAGAACAACAGCACCGGAGATCTGAGCTCTGATCTGAAAACATGGCTGAGCGCCGCCGGAGTTAACCCAGAAACATGCATGGAAGGATTCGAAGGCACCAATAGCATTGTCAAAGGATTAGTTTCCGAAGGCATCAACCAATTAACTTCACAGGTTTATAACCTTCTTTCCATGGTGAAATCGATCTCCAATCAGCCGGCCGGAAAAGGGGAGAGCAAGTTTCCCGCCTGGCTCAAAACGGAGGAACAGAGCCTGCTGCAACAAAATGACTTCGCCGCCGATGCGACTGTGGCCGCCGATGGGACAGGGGATTTCACCACCGTAATGGAGGCGGTGCTGGCAGCCCCCGACAAGAGCATGCGGCGGTATGTGATATACGTGAAAAAGGGTGTGTATTTCGAGAACGTCGAGATCAAGAAGAAGAAATGGAATATCATGATGATCGGAGATGGCATTGACGCCACCGTCATATCCGGTAACCGGAGCTTCATCGACGGCTGGACGACGTTCCGATCAGCCACCTTCGGTAACCCTTTAACTCTCTCTTCCACTTTGATTTGA

Protein sequence

LPHNVIVKATVPFKSTRHLPNLRLRPFLNSLTFPPKSFIYSGSPVSSSPVTSSPVSSSPVSSSPVSSSPVSSSPVSSSPVSSPVSSPNTNHKQSHLNNPILMAKLTKTHLKFWLFMLLILFPMAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGESKFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFGNPLTLSSTLI
Homology
[Close]
BLAST of Cp4.1LG08g12640 vs. Swiss-Prot
Match: PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.9e-85
Identity = 164/259 (63.32%), Postives = 198/259 (76.45%), Query Frame = 1 

Query: 119 ILFPMAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISD 178
           I F      D  QT+CLKV ++EF+ S+  TI  V+QV  I S F    GD RL+ AISD
Sbjct: 33  IPFASCSITDDLQTQCLKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISD 92

Query: 179 CLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSI 238
           CLDLLD S+D+L+WSLSA+QN K KNNSTG LSSDL+TWLSAA VN +TC  GFEGTNSI
Sbjct: 93  CLDLLDFSADELNWSLSASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSI 152

Query: 239 VKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGESKFPAWLKTEEQSLLQQNDFAADAT 298
           V+GL+S G+ Q+TS V  LL+ V   SNQ    G+   P+W+KT+++ LLQ +  + DA 
Sbjct: 153 VQGLISAGLGQVTSLVQELLTQVHPNSNQQGPNGQ--IPSWVKTKDRKLLQADGVSVDAI 212

Query: 299 VAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATV 358
           VA DGTG+FT V +AVLAAPD SMRRYVIY+K+G Y ENVEIKKKKWN+MMIGDG+DAT+
Sbjct: 213 VAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATI 272

Query: 359 ISGNRSFIDGWTTFRSATF 378
           ISGNRSF+DGWTTFRSATF
Sbjct: 273 ISGNRSFVDGWTTFRSATF 289

[Close]
BLAST of Cp4.1LG08g12640 vs. Swiss-Prot
Match: PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=PME32 PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 8.9e-79
Identity = 155/284 (54.58%), Postives = 209/284 (73.59%), Query Frame = 1 

Query: 112 FWLFMLLILFPMA-----PAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKF 171
           F+LF+++I    A      + DL Q ECL+VP  EF+ +  + +  + + + I S F K 
Sbjct: 12  FFLFLIIISLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKK 71

Query: 172 LGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPE 231
            G SR+S AI DC+DLLDS++++LSW +SA+Q+P  K+NSTGD+ SDL+TW+SAA  N +
Sbjct: 72  AGKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQD 131

Query: 232 TCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGK------------GES 291
           TC++GFEGTN I+K +V+ G++++ + V NLL+MV S  ++P  K            G S
Sbjct: 132 TCLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFS 191

Query: 292 KFPAWLKTEEQSLLQQNDFA-ADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGV 351
           KFP+W+K  ++ LLQ ++   ADA VAADGTG+FTT+ +AVLAAPD S +RYVI+VK+GV
Sbjct: 192 KFPSWVKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGV 251

Query: 352 YFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATF 378
           Y ENVEIKKKKWNIMM+GDGIDATVI+GNRSFIDGWTTFRSATF
Sbjct: 252 YVENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATF 295

[Close]
BLAST of Cp4.1LG08g12640 vs. Swiss-Prot
Match: PME44_ARATH (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=PME44 PE=2 SV=2)

HSP 1 Score: 275.8 bits (704), Expect = 7.3e-73
Identity = 149/291 (51.20%), Postives = 199/291 (68.38%), Query Frame = 1 

Query: 110 LKFWLFMLLILFPMAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGD 169
           LK++L +L++   ++   +  Q + LKVP+SEF +S+ + + V+RQV  I S F  F GD
Sbjct: 4   LKYFLILLMLGLCVSSEENF-QYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 63

Query: 170 SRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCM 229
            RL  A+SDCLDLLD SS++L+WS SA++NPK K N TGD+ SD +TWLSAA  N  TCM
Sbjct: 64  RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 123

Query: 230 EGFEGTNSIVKGLVSEGINQLTSQVYNLLSMV------KSISNQ-PAGKGES-------- 289
           EGF+GT+ +VK LV+  ++QL S +  LL +V      K++S   P  KG          
Sbjct: 124 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 183

Query: 290 --------KFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYV 349
                   +FP W++ +++ LL+ N    D +VA DGTG+FT +M+A+  APD S  R+V
Sbjct: 184 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 243

Query: 350 IYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATF 378
           IY+KKG+Y ENVEIKKKKWNI+M+GDGID TVISGNRSFIDGWTTFRSATF
Sbjct: 244 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATF 293

[Close]
BLAST of Cp4.1LG08g12640 vs. Swiss-Prot
Match: PME22_ARATH (Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana GN=PME22 PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.2e-37
Identity = 94/222 (42.34%), Postives = 133/222 (59.91%), Query Frame = 1 

Query: 170 SRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSS--------DLKTWLSAA 229
           SR   AI DC +L+  S  +L+WS+              D  S        +LKTWLSAA
Sbjct: 99  SREQVAIEDCKELVGFSVTELAWSMLEMNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAA 158

Query: 230 GVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQP--AGKGESKF--P 289
             N +TC+EGFEGT    + L+   + Q+T  V N+L M   ++  P  A + ES    P
Sbjct: 159 MSNQDTCLEGFEGTERKYEELIKGSLRQVTQLVSNVLDMYTQLNALPFKASRNESVIASP 218

Query: 290 AWLKTEEQSLLQQNDFAA---DATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVY 349
            WL   ++SL+ ++D +    +  VA DG G + T+ EA+  AP+ S +RYVIYVKKGVY
Sbjct: 219 EWLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVY 278

Query: 350 FENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSAT 377
            EN+++KKKK NIM++GDGI  T+I+G+R+F+ G TTFR+AT
Sbjct: 279 KENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTTFRTAT 320

[Close]
BLAST of Cp4.1LG08g12640 vs. Swiss-Prot
Match: PME22_SOLLC (Pectinesterase 2.2 OS=Solanum lycopersicum GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.9e-34
Identity = 102/316 (32.28%), Postives = 173/316 (54.75%), Query Frame = 1 

Query: 93  QSHLNNPILMAKLTKTHLKFWLFMLLILFPMAPA------ADLEQTE-----CLKVPSSE 152
           ++H  N I+  K+    +  ++ + L++F +AP       ++L +T      CL   S  
Sbjct: 11  KTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIKHSNLCKTAQDSQLCLSYVSDL 70

Query: 153 FSNSVLST------------IGVVRQV---MLIFSPFTKFLGDSRLSTAISDCLDLLDSS 212
            SN +++T            +  V Q+   + + S     + D R   A++DCL+LLD S
Sbjct: 71  MSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQEGALTDCLELLDQS 130

Query: 213 SDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEG-TNSIVKGLVSE 272
            D +S S++A       +  T    ++ ++WLS    N  TC++  +  T +++ G    
Sbjct: 131 VDLVSDSIAAI------DKRTHSEHANAQSWLSGVLTNHVTCLDELDSFTKAMINGT--- 190

Query: 273 GINQLTSQVYNLLSMVKSIS---NQPAGKGESKFPAWLKTEEQSLLQQN--DFAADATVA 332
            +++L S+    L+M+ S++   +     G  K P+W+ + ++ L++ +  D  A+A VA
Sbjct: 191 NLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGANAVVA 250

Query: 333 ADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVIS 377
            DGTG + T+ EAV AAPDKS  RYVIYVK+G+Y ENVE+  +K  +M++GDG+ AT+I+
Sbjct: 251 KDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMHATIIT 310

[Close]
BLAST of Cp4.1LG08g12640 vs. TrEMBL
Match: A0A0A0L4S0_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G000060 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 7.5e-117
Identity = 227/283 (80.21%), Postives = 252/283 (89.05%), Query Frame = 1 

Query: 99  PILMAKLTKTHLKFWLFMLLILFPMA-PAA---DLEQTECLKVPSSEFSNSVLSTIGVVR 158
           PILMAKLT   L+F   +L++LFPM  PA+   DL QTECL VPSS+FSNS+LSTI VVR
Sbjct: 67  PILMAKLTTQTLQFCC-LLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVR 126

Query: 159 QVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDL 218
           QVM IFSPF+K LGD RLSTAISDCLDLLDSS+DQLSWSLSA QNPKAKN+STGDLSSDL
Sbjct: 127 QVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDL 186

Query: 219 KTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGES 278
           KTWLSAA VNPETCM+GFEGTNSI+KGLVS G+NQLTSQ+Y+LLSMVKSI NQP     S
Sbjct: 187 KTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQP-----S 246

Query: 279 KFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVY 338
           +FP+WLK+E+Q+LLQ ND AADATVAADGTGDFT VM+AVLAAPD S+RRYVIY+KKGVY
Sbjct: 247 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 306

Query: 339 FENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATF 378
            ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFIDGWTTFRSATF
Sbjct: 307 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATF 343

[Close]
BLAST of Cp4.1LG08g12640 vs. TrEMBL
Match: M5WFH0_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa004300mg PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.2e-83
Identity = 164/259 (63.32%), Postives = 198/259 (76.45%), Query Frame = 1 

Query: 119 ILFPMAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISD 178
           I F      D  QT+CLKV ++EF+ S+  TI  V+QV  I S F    GD RL+ AISD
Sbjct: 28  IPFASCSITDDLQTQCLKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISD 87

Query: 179 CLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSI 238
           CLDLLD S+D+L+WSLSA+QN K KNNSTG LSSDL+TWLSAA VN +TC  GFEGTNSI
Sbjct: 88  CLDLLDFSADELNWSLSASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSI 147

Query: 239 VKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGESKFPAWLKTEEQSLLQQNDFAADAT 298
           V+GL+S G+ Q+TS V  LL+ V   SNQ    G+   P+W+KT+++ LLQ +  + DA 
Sbjct: 148 VQGLISAGLGQVTSLVQELLTQVHPNSNQQGPNGQ--IPSWVKTKDRKLLQADGVSVDAI 207

Query: 299 VAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATV 358
           VA DGTG+FT V +AVLAAPD SMRRYVIY+K+G Y ENVEIKKKKWN+MMIGDG+DAT+
Sbjct: 208 VAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYRENVEIKKKKWNLMMIGDGMDATI 267

Query: 359 ISGNRSFIDGWTTFRSATF 378
           ISGNRSF+DGWTTFRSATF
Sbjct: 268 ISGNRSFVDGWTTFRSATF 284

[Close]
BLAST of Cp4.1LG08g12640 vs. TrEMBL
Match: S5MHJ3_9ROSA (Pectinesterase (Fragment) OS=Pyrus x bretschneideri GN=PME4 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.5e-82
Identity = 158/247 (63.97%), Postives = 196/247 (79.35%), Query Frame = 1 

Query: 131 QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 190
           ++ECLKVP+SEF+ S+  TIG V+QV  I S F    GD  LS AISDCLDLLD ++D+L
Sbjct: 3   RSECLKVPASEFAGSLRDTIGAVQQVASILSQFATAFGDFHLSNAISDCLDLLDFTADEL 62

Query: 191 SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 250
           +WSLSA+QNP+ K+NSTG LSSDL+TWLSAA VN +TC +GFEGTNSIVKGLV+ G+NQ+
Sbjct: 63  NWSLSASQNPEGKDNSTGKLSSDLRTWLSAALVNQDTCSDGFEGTNSIVKGLVTTGLNQV 122

Query: 251 TSQVYNLLSMVKSISNQPAGKGESKFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTV 310
           TS V  LL+ V+  ++     GE   P+W+K E++ LLQ      DA VA DGTG+FT V
Sbjct: 123 TSLVQGLLTQVQPNTDHHGPNGE--IPSWVKAEDRKLLQAGGVNVDAVVAQDGTGNFTNV 182

Query: 311 MEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWT 370
            +AVLAAPD+SM RYVIY+K+G Y ENVEIKKKKWN+MMIGDG+DAT+ISG+R+F+DGWT
Sbjct: 183 TDAVLAAPDESMTRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGSRNFVDGWT 242

Query: 371 TFRSATF 378
           TFRSATF
Sbjct: 243 TFRSATF 247

[Close]
BLAST of Cp4.1LG08g12640 vs. TrEMBL
Match: R9QPU2_GOSBA (Pectinesterase OS=Gossypium barbadense PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 4.6e-82
Identity = 165/262 (62.98%), Postives = 201/262 (76.72%), Query Frame = 1 

Query: 131 QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 190
           +TECLKVP++EF  S+ +TI  +R+   + S F  F  D RLS AISDCLDLLDSS+D+L
Sbjct: 32  ETECLKVPATEFIGSLKTTIDAIRKATSVVSQFGGFFHDFRLSNAISDCLDLLDSSADEL 91

Query: 191 SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 250
           SW++SA+QNP AK+NSTGDLSSDL+TWLSAA VN +TC++GFEGTNS+VK +VS  +NQ+
Sbjct: 92  SWTMSASQNPNAKDNSTGDLSSDLRTWLSAAMVNQQTCIDGFEGTNSMVKTVVSGSLNQI 151

Query: 251 TSQVYNLLSMV----KSISN------QPAG-----KGESKFPAWLKTEEQSLLQQNDFAA 310
           TS V NLL MV     S SN      Q  G      G+S+FP W K E++ LLQ N   A
Sbjct: 152 TSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGGGHPGQSRFPVWFKREDRRLLQINGVTA 211

Query: 311 DATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGID 370
           +  VAADG+G+FT +M+AV  APDKSM RYVIY+KKG+Y ENVEIKKKKWN++MIGDG+D
Sbjct: 212 NVVVAADGSGNFTRIMDAVETAPDKSMNRYVIYIKKGLYKENVEIKKKKWNLVMIGDGMD 271

Query: 371 ATVISGNRSFIDGWTTFRSATF 378
            TVISGNRSFIDGWTTFRSATF
Sbjct: 272 VTVISGNRSFIDGWTTFRSATF 293

[Close]
BLAST of Cp4.1LG08g12640 vs. TrEMBL
Match: A0ZNK2_PYRCO (Pectinesterase OS=Pyrus communis GN=PcPME4 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.0e-81
Identity = 158/247 (63.97%), Postives = 195/247 (78.95%), Query Frame = 1 

Query: 131 QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 190
           ++ECLKVP+SEF+ S+  TIG V+QV  I S F    GD  LS AISDCLDLLD ++D+L
Sbjct: 33  RSECLKVPASEFAGSLRDTIGAVQQVASILSQFATAFGDFHLSNAISDCLDLLDFTADEL 92

Query: 191 SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 250
           +WSLSA+QNP+ K+NSTG LSSDL+TWLSAA VN +TC +GFEGTNSIVKGLV+ G+NQ+
Sbjct: 93  NWSLSASQNPEGKDNSTGKLSSDLRTWLSAALVNQDTCSDGFEGTNSIVKGLVTTGLNQV 152

Query: 251 TSQVYNLLSMVKSISNQPAGKGESKFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTV 310
           TS V  LL+ V+  ++     GE   P+W+K E++ LLQ      DA VA DGTG+FT V
Sbjct: 153 TSLVQGLLTQVQPNTDHHGPNGE--IPSWVKAEDRKLLQAGGVNVDAVVAQDGTGNFTNV 212

Query: 311 MEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWT 370
            +AVLAAPD+SM RYVIY+K G Y ENVEIKKKKWN+MMIGDG+DAT+ISG+R+F+DGWT
Sbjct: 213 TDAVLAAPDESMTRYVIYIKGGTYKENVEIKKKKWNLMMIGDGMDATIISGSRNFVDGWT 272

Query: 371 TFRSATF 378
           TFRSATF
Sbjct: 273 TFRSATF 277

[Close]
BLAST of Cp4.1LG08g12640 vs. TAIR10
Match: AT3G43270.1 (AT3G43270.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 295.4 bits (755), Expect = 5.0e-80
Identity = 155/284 (54.58%), Postives = 209/284 (73.59%), Query Frame = 1 

Query: 112 FWLFMLLILFPMA-----PAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKF 171
           F+LF+++I    A      + DL Q ECL+VP  EF+ +  + +  + + + I S F K 
Sbjct: 12  FFLFLIIISLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKK 71

Query: 172 LGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPE 231
            G SR+S AI DC+DLLDS++++LSW +SA+Q+P  K+NSTGD+ SDL+TW+SAA  N +
Sbjct: 72  AGKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQD 131

Query: 232 TCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGK------------GES 291
           TC++GFEGTN I+K +V+ G++++ + V NLL+MV S  ++P  K            G S
Sbjct: 132 TCLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFS 191

Query: 292 KFPAWLKTEEQSLLQQNDFA-ADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGV 351
           KFP+W+K  ++ LLQ ++   ADA VAADGTG+FTT+ +AVLAAPD S +RYVI+VK+GV
Sbjct: 192 KFPSWVKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGV 251

Query: 352 YFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATF 378
           Y ENVEIKKKKWNIMM+GDGIDATVI+GNRSFIDGWTTFRSATF
Sbjct: 252 YVENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATF 295

[Close]
BLAST of Cp4.1LG08g12640 vs. TAIR10
Match: AT4G33220.1 (AT4G33220.1 pectin methylesterase 44)

HSP 1 Score: 275.8 bits (704), Expect = 4.1e-74
Identity = 149/291 (51.20%), Postives = 199/291 (68.38%), Query Frame = 1 

Query: 110 LKFWLFMLLILFPMAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGD 169
           LK++L +L++   ++   +  Q + LKVP+SEF +S+ + + V+RQV  I S F  F GD
Sbjct: 4   LKYFLILLMLGLCVSSEENF-QYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 63

Query: 170 SRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCM 229
            RL  A+SDCLDLLD SS++L+WS SA++NPK K N TGD+ SD +TWLSAA  N  TCM
Sbjct: 64  RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 123

Query: 230 EGFEGTNSIVKGLVSEGINQLTSQVYNLLSMV------KSISNQ-PAGKGES-------- 289
           EGF+GT+ +VK LV+  ++QL S +  LL +V      K++S   P  KG          
Sbjct: 124 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 183

Query: 290 --------KFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYV 349
                   +FP W++ +++ LL+ N    D +VA DGTG+FT +M+A+  APD S  R+V
Sbjct: 184 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 243

Query: 350 IYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATF 378
           IY+KKG+Y ENVEIKKKKWNI+M+GDGID TVISGNRSFIDGWTTFRSATF
Sbjct: 244 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATF 293

[Close]
BLAST of Cp4.1LG08g12640 vs. TAIR10
Match: AT3G05620.1 (AT3G05620.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 157.9 bits (398), Expect = 1.2e-38
Identity = 94/222 (42.34%), Postives = 133/222 (59.91%), Query Frame = 1 

Query: 170 SRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSS--------DLKTWLSAA 229
           SR   AI DC +L+  S  +L+WS+              D  S        +LKTWLSAA
Sbjct: 99  SREQVAIEDCKELVGFSVTELAWSMLEMNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAA 158

Query: 230 GVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQP--AGKGESKF--P 289
             N +TC+EGFEGT    + L+   + Q+T  V N+L M   ++  P  A + ES    P
Sbjct: 159 MSNQDTCLEGFEGTERKYEELIKGSLRQVTQLVSNVLDMYTQLNALPFKASRNESVIASP 218

Query: 290 AWLKTEEQSLLQQNDFAA---DATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVY 349
            WL   ++SL+ ++D +    +  VA DG G + T+ EA+  AP+ S +RYVIYVKKGVY
Sbjct: 219 EWLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVY 278

Query: 350 FENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSAT 377
            EN+++KKKK NIM++GDGI  T+I+G+R+F+ G TTFR+AT
Sbjct: 279 KENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTTFRTAT 320

[Close]
BLAST of Cp4.1LG08g12640 vs. TAIR10
Match: AT3G14310.1 (AT3G14310.1 pectin methylesterase 3)

HSP 1 Score: 135.2 bits (339), Expect = 8.7e-32
Identity = 92/241 (38.17%), Postives = 127/241 (52.70%), Query Frame = 1 

Query: 171 RLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCME 230
           R  TA+ DCL+ +D + D+L  ++        K  +  + + DLKT +S+A  N ETC++
Sbjct: 122 REKTALHDCLETIDETLDELHETVEDLHLYPTKK-TLREHAGDLKTLISSAITNQETCLD 181

Query: 231 GF---EGTNSIVKGLVSEGI--------------NQLTSQVYNLLSMVKSISNQP----- 290
           GF   +    + K L+   I              N   + + N     K  SN       
Sbjct: 182 GFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEE 241

Query: 291 ----------AGKGE---SKFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVL 350
                     AG GE     +P WL   ++ LLQ +   ADATVAADG+G F TV  AV 
Sbjct: 242 NQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVA 301

Query: 351 AAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSA 377
           AAP+ S +RYVI++K GVY ENVE+ KKK NIM +GDG   T+I+G+R+ +DG TTF SA
Sbjct: 302 AAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSA 361

[Close]
BLAST of Cp4.1LG08g12640 vs. TAIR10
Match: AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-31
Identity = 86/230 (37.39%), Postives = 127/230 (55.22%), Query Frame = 1 

Query: 174 TAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGF- 233
           TA+ DCL+ +D + D+L  ++        K  S    + DLKT +S+A  N  TC++GF 
Sbjct: 128 TALHDCLETIDETLDELHVAVEDLHQ-YPKQKSLRKHADDLKTLISSAITNQGTCLDGFS 187

Query: 234 -EGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGESKFPA------------- 293
            +  +  V+  + +G   +     N L+M+K+++       E +  +             
Sbjct: 188 YDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKE 247

Query: 294 ------------WLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYV 353
                       WL   ++ LLQ +   ADATVA DG+GDFTTV  AV AAP+KS +R+V
Sbjct: 248 VTGDLDSDGWPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFV 307

Query: 354 IYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSAT 377
           I++K GVY ENVE+ KKK NIM +GDG   T+I+G+R+ +DG TTF SAT
Sbjct: 308 IHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSAT 356

[Close]
BLAST of Cp4.1LG08g12640 vs. NCBI nr
Match: gi|778674515|ref|XP_004146188.2| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus])

HSP 1 Score: 428.7 bits (1101), Expect = 1.1e-116
Identity = 227/283 (80.21%), Postives = 252/283 (89.05%), Query Frame = 1 

Query: 99  PILMAKLTKTHLKFWLFMLLILFPMA-PAA---DLEQTECLKVPSSEFSNSVLSTIGVVR 158
           PILMAKLT   L+F   +L++LFPM  PA+   DL QTECL VPSS+FSNS+LSTI VVR
Sbjct: 67  PILMAKLTTQTLQFCC-LLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVR 126

Query: 159 QVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDL 218
           QVM IFSPF+K LGD RLSTAISDCLDLLDSS+DQLSWSLSA QNPKAKN+STGDLSSDL
Sbjct: 127 QVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDL 186

Query: 219 KTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGES 278
           KTWLSAA VNPETCM+GFEGTNSI+KGLVS G+NQLTSQ+Y+LLSMVKSI NQP     S
Sbjct: 187 KTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQP-----S 246

Query: 279 KFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVY 338
           +FP+WLK+E+Q+LLQ ND AADATVAADGTGDFT VM+AVLAAPD S+RRYVIY+KKGVY
Sbjct: 247 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 306

Query: 339 FENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATF 378
            ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFIDGWTTFRSATF
Sbjct: 307 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATF 343

[Close]
BLAST of Cp4.1LG08g12640 vs. NCBI nr
Match: gi|659095151|ref|XP_008448427.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo])

HSP 1 Score: 427.2 bits (1097), Expect = 3.1e-116
Identity = 224/282 (79.43%), Postives = 248/282 (87.94%), Query Frame = 1 

Query: 99  PILMAKLTKTHLKFWLFMLLI---LFPMAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQ 158
           PILMAKLT   L+F    +L+   L P +  ADL QTECL VPSS+FSNS+LSTI VVRQ
Sbjct: 67  PILMAKLTTQILQFCCIFILLFPMLLPASSPADLVQTECLSVPSSQFSNSLLSTIDVVRQ 126

Query: 159 VMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLK 218
           VM IFSPF+K LGD RLSTAISDCLDLLDSS+DQLSWSLSA QNPKAK++STGDLSSDLK
Sbjct: 127 VMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLK 186

Query: 219 TWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGESK 278
           TWLSAA VNPETCM+GFEGTNSI+KGLVS GINQLTSQ+Y+LLSMVKSI NQP     S+
Sbjct: 187 TWLSAAVVNPETCMDGFEGTNSIIKGLVSGGINQLTSQLYDLLSMVKSIPNQP-----SE 246

Query: 279 FPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYF 338
           FP+WLK+E+++LLQ ND AADATVAADGTGDFT VM+AVLAAPDKS RRYVIY+KKGVY 
Sbjct: 247 FPSWLKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDKSTRRYVIYIKKGVYL 306

Query: 339 ENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATF 378
           ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFIDGWTTFRSATF
Sbjct: 307 ENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATF 343

[Close]
BLAST of Cp4.1LG08g12640 vs. NCBI nr
Match: gi|1009142907|ref|XP_015888976.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B isoform X2 [Ziziphus jujuba])

HSP 1 Score: 325.1 bits (832), Expect = 1.7e-85
Identity = 170/271 (62.73%), Postives = 211/271 (77.86%), Query Frame = 1 

Query: 114 LFMLLILF------PMAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFL 173
           LF++++L         +   DL   ECLKV  +EF+++V  TI  V+QV+ I SPF    
Sbjct: 16  LFLVVVLLCACCGSGTSTTTDLLHAECLKVSPAEFASTVKDTIDTVQQVVSILSPFANTF 75

Query: 174 GDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPET 233
           GDSRLS AISDCLDLL+ SSD+LSWS+SA QNPK K+NSTG+LSSDL+TWLSAA +N ET
Sbjct: 76  GDSRLSNAISDCLDLLEFSSDELSWSVSATQNPKGKDNSTGNLSSDLRTWLSAALINQET 135

Query: 234 CMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSI-SNQPAGKGESKFPAWLKTEEQS 293
           C +GFEGT+SIVKGLVS G++Q+TS V NLL+ V  + S QP  K   +FP+W+ T ++ 
Sbjct: 136 CSDGFEGTSSIVKGLVSSGLSQVTSLVQNLLTQVHPVKSEQPTKK--DRFPSWVITRDRK 195

Query: 294 LLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWN 353
           LLQ N  + D  VA+DG+G+FT VM+AVLAAPD SM+RYVIY+K+G+Y ENVEIKKKKWN
Sbjct: 196 LLQANGVSVDVVVASDGSGNFTNVMDAVLAAPDYSMKRYVIYIKRGIYKENVEIKKKKWN 255

Query: 354 IMMIGDGIDATVISGNRSFIDGWTTFRSATF 378
           +MMIGDG+ ATVISGNRSFIDGWTTFRSATF
Sbjct: 256 LMMIGDGMGATVISGNRSFIDGWTTFRSATF 284

[Close]
BLAST of Cp4.1LG08g12640 vs. NCBI nr
Match: gi|1009142905|ref|XP_015888975.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B isoform X1 [Ziziphus jujuba])

HSP 1 Score: 325.1 bits (832), Expect = 1.7e-85
Identity = 170/271 (62.73%), Postives = 211/271 (77.86%), Query Frame = 1 

Query: 114 LFMLLILF------PMAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFL 173
           LF++++L         +   DL   ECLKV  +EF+++V  TI  V+QV+ I SPF    
Sbjct: 16  LFLVVVLLCACCGSGTSTTTDLLHAECLKVSPAEFASTVKDTIDTVQQVVSILSPFANTF 75

Query: 174 GDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPET 233
           GDSRLS AISDCLDLL+ SSD+LSWS+SA QNPK K+NSTG+LSSDL+TWLSAA +N ET
Sbjct: 76  GDSRLSNAISDCLDLLEFSSDELSWSVSATQNPKGKDNSTGNLSSDLRTWLSAALINQET 135

Query: 234 CMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSI-SNQPAGKGESKFPAWLKTEEQS 293
           C +GFEGT+SIVKGLVS G++Q+TS V NLL+ V  + S QP  K   +FP+W+ T ++ 
Sbjct: 136 CSDGFEGTSSIVKGLVSSGLSQVTSLVQNLLTQVHPVKSEQPTKK--DRFPSWVITRDRK 195

Query: 294 LLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWN 353
           LLQ N  + D  VA+DG+G+FT VM+AVLAAPD SM+RYVIY+K+G+Y ENVEIKKKKWN
Sbjct: 196 LLQANGVSVDVVVASDGSGNFTNVMDAVLAAPDYSMKRYVIYIKRGIYKENVEIKKKKWN 255

Query: 354 IMMIGDGIDATVISGNRSFIDGWTTFRSATF 378
           +MMIGDG+ ATVISGNRSFIDGWTTFRSATF
Sbjct: 256 LMMIGDGMGATVISGNRSFIDGWTTFRSATF 284

[Close]
BLAST of Cp4.1LG08g12640 vs. NCBI nr
Match: gi|645274002|ref|XP_008242144.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B [Prunus mume])

HSP 1 Score: 324.3 bits (830), Expect = 2.9e-85
Identity = 168/259 (64.86%), Postives = 200/259 (77.22%), Query Frame = 1 

Query: 119 ILFPMAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISD 178
           I F      D  QT CLKVP+SEF+ S+  TI  V+QV  I SPF    GD RLS AISD
Sbjct: 28  IPFASCSITDDLQTGCLKVPASEFAGSLKDTIDAVQQVASILSPFANAFGDFRLSNAISD 87

Query: 179 CLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSI 238
           CLDLLD S+D+L+WS+SA+QN K KNNSTG LSSDL+TWLSAA VN +TC  GFEGTNSI
Sbjct: 88  CLDLLDFSADELNWSISASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSI 147

Query: 239 VKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGESKFPAWLKTEEQSLLQQNDFAADAT 298
           V+GLVS G++Q+TS V  LL+ V   SNQ    G+   P+W+KTE++ LLQ +  + DA 
Sbjct: 148 VQGLVSAGLDQVTSLVQELLTQVHPNSNQQGPNGQ--IPSWVKTEDRKLLQADGVSVDAI 207

Query: 299 VAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATV 358
           VA DGTG+FT V +AVLAAPD SMRRYVIY+K+G Y ENVEIKKKKWN+MMIGDG+DAT+
Sbjct: 208 VAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATI 267

Query: 359 ISGNRSFIDGWTTFRSATF 378
           ISGNR+F+DGWTTFRSATF
Sbjct: 268 ISGNRNFVDGWTTFRSATF 284

The following BLAST results are available for this feature:
BLAST of Cp4.1LG08g12640 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME_PRUPE2.9e-8563.32Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1[more]
PME32_ARATH8.9e-7954.58Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=P... [more]
PME44_ARATH7.3e-7351.20Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=P... [more]
PME22_ARATH2.2e-3742.34Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana GN=P... [more]
PME22_SOLLC3.9e-3432.28Pectinesterase 2.2 OS=Solanum lycopersicum GN=PME2.2 PE=3 SV=1[more]
BLAST of Cp4.1LG08g12640 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L4S0_CUCSA7.5e-11780.21Pectinesterase OS=Cucumis sativus GN=Csa_3G000060 PE=4 SV=1[more]
M5WFH0_PRUPE3.2e-8363.32Pectinesterase OS=Prunus persica GN=PRUPE_ppa004300mg PE=4 SV=1[more]
S5MHJ3_9ROSA3.5e-8263.97Pectinesterase (Fragment) OS=Pyrus x bretschneideri GN=PME4 PE=2 SV=1[more]
R9QPU2_GOSBA4.6e-8262.98Pectinesterase OS=Gossypium barbadense PE=2 SV=1[more]
A0ZNK2_PYRCO1.0e-8163.97Pectinesterase OS=Pyrus communis GN=PcPME4 PE=2 SV=1[more]
BLAST of Cp4.1LG08g12640 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G43270.15.0e-8054.58 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G33220.14.1e-7451.20 pectin methylesterase 44[more]
AT3G05620.11.2e-3842.34 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G14310.18.7e-3238.17 pectin methylesterase 3[more]
AT1G53830.11.1e-3137.39 pectin methylesterase 2[more]
BLAST of Cp4.1LG08g12640 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778674515|ref|XP_004146188.2|1.1e-11680.21PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus][more]
gi|659095151|ref|XP_008448427.1|3.1e-11679.43PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo][more]
gi|1009142907|ref|XP_015888976.1|1.7e-8562.73PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B isoform X2 [Ziziphus ju... [more]
gi|1009142905|ref|XP_015888975.1|1.7e-8562.73PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B isoform X1 [Ziziphus ju... [more]
gi|645274002|ref|XP_008242144.1|2.9e-8564.86PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B [Prunus mume][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006501Pectinesterase_inhib_dom
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0050829 defense response to Gram-negative bacterium
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g12640.1Cp4.1LG08g12640.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 296..378
score: 6.4
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 168..265
score: 1.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 171..258
score: 1.2
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 125..264
score: 1.9
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 155..265
score: 4.45
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 294..377
score: 1.35
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 294..377
score: 2.7
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 75..377
score: 2.2E
NoneNo IPR availablePANTHERPTHR31707:SF10PECTINESTERASE/PECTINESTERASE INHIBITOR 32-RELATEDcoord: 75..377
score: 2.2E