CsaV3_3G000050 (gene) Cucumber (Chinese Long) v3

NameCsaV3_3G000050
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
DescriptionPectinesterase
Locationchr3 : 181308 .. 185285 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTAAGAACTAAGTGATTCAATAGAAAGCGTTTATTTTATTTAAAACATTTAAAATTGTAGTGTTTACAATGTTGGTTTTGTCCAAATACAATAGAAATGAAAATAAAGAAATAGAATATATTAATGAGGGGGAAGGGATGGATGAAGAGGAAGCGAAAGTCAGGGTGGGAATGGTAGTCAACTTCTATCATTTAATAGCATTTGTAGTTAGGAGAGGAGAGAGTGATATGGGCATTTGTTTTTGGTTCAAAAGAAGAGTAATTAGGAGAGACGAGTATGGGTGTGGATGATGACACTGACATCCAAAAAGATCAAAGGGAGATGTTCGAATCTACTAAAGTTGTCGTAAATGCTTTCCAGCGACTGTGAGTAAGATGCCCCGAGATCCGCGTAAGAAGAGAAGCTTTTTATGAGCGCCGACACATGGATTTTCACACAAACCCTTCCCCACAAAACGTGAATGTAGCATTCAAATCAACGCGCTATCTCCCCAACCCCCTGCCTTTAAAGTTCCTTTCCCTCACCTTCCAACTGGACTCTTCATTTACTTCACCGATTTTTGTTTTAATTTTGCTTCATCATCCCATACATACTGGCCAGGCCAAAGGCAGCTCAATAATTCCTATCTTAATGGCCAAATTAACTACACAAACCCTTCAGTTTTGCTGCCTCTTGATCCTTTTGTTTCCCATGCTGCTTCCGGCCAGCTCTCCACCGGATCTTGTGCAGACAGAGTGCTTGAGCGTACCCTCCTCCCAGTTCTCCAACTCACTCCTCTCCACCATCGATGTTGTACGACAGGTTATGGCCATTTTCTCTCCGTTCAGTAAATTACTGGGAGACTTCCGGCTTTCCACCGCCATTTCCGACTGTCTCGATTTGCTTGACTCCTCTGCTGACCAGTTGAGCTGGTCCCTCTCCGCCACCCAGAATCCCAAGGGTACACCATTATTTACAATACCCCACCCCCACCGCATTCTTATAAATTTGCATATTAACTCTCTACCCCCTAAGCCTATTCATTTATATGTTGCGCTTTCAAACTTTGCTTTAATATGTGTTGTTGGTTAGTTCACGGGGATATTGTCGGTTGTGCTGCTGCTGGACCTACTAGTTACTTTTATAAATTGCGCATGCAAATGCAACTACACTAGAAATTCAACATATTTACAAATTAAACATCCTCCTGCTTACATTATTTCAGCTAAAAACCATAGCACGGGAGATCTGAGTTCTGATCTGAAGACATGGCTGAGCGCTGCAGTGGTTAATCCGGAAACATGCATGGATGGATTTGAGGGCACCAATAGCATTATCAAAGGTCTAGTATCTGGAGGCGTTAACCAGTTAACTTCACAGCTCTATGACCTTCTTTCCATGGTGAAATCAATCCCCAATCAACCGAGCGAGTTTCCTTCATGGCTGAAATCTGAGGACCAGAACTTGCTGCAAATAAATGACTTGGCCGCCGATGCCACAGTGGCCGCCGACGGGACTGGGGATTTCACCAATGTGATGGATGCGGTACTAGCAGCCCCTGACAATAGCATACGACGTTATGTCATATACATAAAGAAGGGTGTGTACTTGGAGAACGTGGAGATCAAGAAGAAGAAGTGGAATCTCATGATGATTGGGGATGGAATAGATGCCACCATCATATCGGGTAACCGCAGTTTTATTGACGGTTGGACAACATTCCGATCAGCGACCTTCGGTAATTTTCCCTTTCATTATAATAATTTCTGAGGACAAAACAAAACAGAGAAATGAAGAAGAAGTGGTTATTAATTAATTTGCAAATGATTTGCAGCTGTAAGTGGAAGAGGATTCATAGCGCGAGACATAACATTCGAGAATACTGCAGGAGCAGAGAAACATCAAGCAGTGGCACTTAGATCGGACTCCGATCTGTCGGTGTTTTTTAGATGTCGAATTAGAGGCTACCAAGACACGCTGTATACGCACACCATGCGCCAATTTTACCGCGAGTGCCAAATCAGTGGGACCGTTGATTTTCTTTTCGGCGATGCCACCGTCGTTTTTCAAAACTGCAGCATCCTGGCGAAGAAGGGTCTTCCCAACCAGAAAAACACCATCACTGCTCAGGGCCGGAAAGACCCAAACCAACCCACCGGTTTCTCCATTCAATTCTGCAACATATCTGCGGATTCAGACCTTAAACCCTCGGTTAACACTACTGCTACGTACCTTGGAAGACCATGGAAGGAATATTCCAGAACCATTATCATGCAATCATACATCAGCGATGCCATTAGGCCAGAAGGTTGGCTTGAGTGGAACGCCAATTTTGCATTGAATACACTATTCTACGCTGAGTTTATGAATTACGGACCGGGGGCGGGATTGGCCAAGCGTGTCAATTGGCCCGGTTACCACCGACTGAATCAAACCAGTGAAGCTACTAACTTCACCGTGGCTCAATTCATTGAAGGAAACCTGTGGCTGCCGTCCACGGGTGTCAAATACACTGCAGGACTGGGAGCTAATTAGTAAATCAACAATATGTGGTGCATTGATGCTCCCTCCTATATATCTCACTTTCAAAATATACATAAAAATGAATTAAAACATTAAAAAAAAAAATCCCTTGCCGTAGTTTAGATGATACAATTCGGTGGACCCAGAAAGTTAAGTGCCTCTAGATATTTTTGGTGTTGTAATTAAAGCAAACCCCCTAAATTCCCCTCGAGTCTTCAGTTCTGTGTGTGCCACCGTGGTGCACTTGCCATATGCTTTTGTGTGACCATTTATCTCTTCACTTTGACCTTCATTTTGTAACATGTTGTTCTAGTGCTTTTGGTCGCAATTGTCCATTGTTTTCTGCCCCTCTTTTCATTACCATCGTATTATTGTTTGATGTTTGTTTAATTGTCATATTGCTCTTCGTCTCTGTCTTTCATCTATTATATATATGTTGTGGTTTTTCTTGCCTTCGTGGTCTTTATTATATCGTTCATGTTGATATCACCATGCAGCAACTTTGCTCATCTTTCATTATTGTGTCATCATTTGTTAAAATCATCAATATGAATGTTGGTTAAAAAAATAGATTTGAAGAGGTAATATCTAGTAAAATTTTAATAGTTATTAGTTTCGATATAATAGTACTTAACCTAATCAGTAAATTAATAACTACTTTTTAGTACCATGACGACATTGCCGGTATGACCATAACTCAAATCTTAACTAACATCAAGCATCTACATATAGTAGATGGCGTTAAATGTATCAATTTGAGATTAAAAGTTTCAACCTCTCGTCAAAGGAAAAAAAATGTATTCAAATTTTAGTTTTAAGTTACTAAAATTTTAAATAGAATAAAAGTGGTGGTTGTTTGAAGAATCCACATTTGGCTCCTCGAAATTCTGCTGTTGATGTAAAAAAATGAAAGCTTTTTACTGCAATCACTGGGTATCGATGGTCTAAGATTTCATGCAAAGACCAGCTTCACGAAGACAGAATCTGGAAGATGAAAGAAGATCGACGGAGGGGAAGAAGATATTGTAGAAAGAATATTGCAAAGAGATCTACAAATGAGAAGAATATGAAGAAGATCGAGAAGCATTGAGAATCGAGGAGATGAAAGAGAGAATTGAGAGGAGTCCTTTGCTACTTTTACATTTTTATGATGATGTAAGCGAAAAACCTAGAACTTACATTTTAGAAAAGTAGGTCAATTTCACTTGGGTATTTCAAATTAGGTCATCTGTGAAAAGGATAACCTAAGCATGCATTGCATAAACAACCCAAGTTTAAACCTTCGATTTTGCCTGTAGCTCCGCAAATCTTCAAAAAACCGAGTAGCATACCCAGTTTTTCGGTAAACCCTAGCGAGTTGGTTTTCTTCGATTTCCTCCACTCTCATGGTTCTGTTGCTTTCTTGCATAATTCTCAATTGCAAATCTAATTATAAGCAAAGTAAAAAGAATATTACAAAAATAATATCCCAAA

mRNA sequence

ATGGATTTTCACACAAACCCTTCCCCACAAAACGTGAATGTAGCATTCAAATCAACGCGCTATCTCCCCAACCCCCTGCCTTTAAAGTTCCTTTCCCTCACCTTCCAACTGGACTCTTCATTTACTTCACCGATTTTTGTTTTAATTTTGCTTCATCATCCCATACATACTGGCCAGGCCAAAGGCAGCTCAATAATTCCTATCTTAATGGCCAAATTAACTACACAAACCCTTCAGTTTTGCTGCCTCTTGATCCTTTTGTTTCCCATGCTGCTTCCGGCCAGCTCTCCACCGGATCTTGTGCAGACAGAGTGCTTGAGCGTACCCTCCTCCCAGTTCTCCAACTCACTCCTCTCCACCATCGATGTTGTACGACAGGTTATGGCCATTTTCTCTCCGTTCAGTAAATTACTGGGAGACTTCCGGCTTTCCACCGCCATTTCCGACTGTCTCGATTTGCTTGACTCCTCTGCTGACCAGTTGAGCTGGTCCCTCTCCGCCACCCAGAATCCCAAGGCTAAAAACCATAGCACGGGAGATCTGAGTTCTGATCTGAAGACATGGCTGAGCGCTGCAGTGGTTAATCCGGAAACATGCATGGATGGATTTGAGGGCACCAATAGCATTATCAAAGGTCTAGTATCTGGAGGCGTTAACCAGTTAACTTCACAGCTCTATGACCTTCTTTCCATGGTGAAATCAATCCCCAATCAACCGAGCGAGTTTCCTTCATGGCTGAAATCTGAGGACCAGAACTTGCTGCAAATAAATGACTTGGCCGCCGATGCCACAGTGGCCGCCGACGGGACTGGGGATTTCACCAATGTGATGGATGCGGTACTAGCAGCCCCTGACAATAGCATACGACGTTATGTCATATACATAAAGAAGGGTGTGTACTTGGAGAACGTGGAGATCAAGAAGAAGAAGTGGAATCTCATGATGATTGGGGATGGAATAGATGCCACCATCATATCGGGTAACCGCAGTTTTATTGACGGTTGGACAACATTCCGATCAGCGACCTTCGCTGTAAGTGGAAGAGGATTCATAGCGCGAGACATAACATTCGAGAATACTGCAGGAGCAGAGAAACATCAAGCAGTGGCACTTAGATCGGACTCCGATCTGTCGGTGTTTTTTAGATGTCGAATTAGAGGCTACCAAGACACGCTGTATACGCACACCATGCGCCAATTTTACCGCGAGTGCCAAATCAGTGGGACCGTTGATTTTCTTTTCGGCGATGCCACCGTCGTTTTTCAAAACTGCAGCATCCTGGCGAAGAAGGGTCTTCCCAACCAGAAAAACACCATCACTGCTCAGGGCCGGAAAGACCCAAACCAACCCACCGGTTTCTCCATTCAATTCTGCAACATATCTGCGGATTCAGACCTTAAACCCTCGGTTAACACTACTGCTACGTACCTTGGAAGACCATGGAAGGAATATTCCAGAACCATTATCATGCAATCATACATCAGCGATGCCATTAGGCCAGAAGGTTGGCTTGAGTGGAACGCCAATTTTGCATTGAATACACTATTCTACGCTGAGTTTATGAATTACGGACCGGGGGCGGGATTGGCCAAGCGTGTCAATTGGCCCGGTTACCACCGACTGAATCAAACCAGTGAAGCTACTAACTTCACCGTGGCTCAATTCATTGAAGGAAACCTGTGGCTGCCGTCCACGGGTGTCAAATACACTGCAGGACTGGGAGCTAATTAG

Coding sequence (CDS)

ATGGATTTTCACACAAACCCTTCCCCACAAAACGTGAATGTAGCATTCAAATCAACGCGCTATCTCCCCAACCCCCTGCCTTTAAAGTTCCTTTCCCTCACCTTCCAACTGGACTCTTCATTTACTTCACCGATTTTTGTTTTAATTTTGCTTCATCATCCCATACATACTGGCCAGGCCAAAGGCAGCTCAATAATTCCTATCTTAATGGCCAAATTAACTACACAAACCCTTCAGTTTTGCTGCCTCTTGATCCTTTTGTTTCCCATGCTGCTTCCGGCCAGCTCTCCACCGGATCTTGTGCAGACAGAGTGCTTGAGCGTACCCTCCTCCCAGTTCTCCAACTCACTCCTCTCCACCATCGATGTTGTACGACAGGTTATGGCCATTTTCTCTCCGTTCAGTAAATTACTGGGAGACTTCCGGCTTTCCACCGCCATTTCCGACTGTCTCGATTTGCTTGACTCCTCTGCTGACCAGTTGAGCTGGTCCCTCTCCGCCACCCAGAATCCCAAGGCTAAAAACCATAGCACGGGAGATCTGAGTTCTGATCTGAAGACATGGCTGAGCGCTGCAGTGGTTAATCCGGAAACATGCATGGATGGATTTGAGGGCACCAATAGCATTATCAAAGGTCTAGTATCTGGAGGCGTTAACCAGTTAACTTCACAGCTCTATGACCTTCTTTCCATGGTGAAATCAATCCCCAATCAACCGAGCGAGTTTCCTTCATGGCTGAAATCTGAGGACCAGAACTTGCTGCAAATAAATGACTTGGCCGCCGATGCCACAGTGGCCGCCGACGGGACTGGGGATTTCACCAATGTGATGGATGCGGTACTAGCAGCCCCTGACAATAGCATACGACGTTATGTCATATACATAAAGAAGGGTGTGTACTTGGAGAACGTGGAGATCAAGAAGAAGAAGTGGAATCTCATGATGATTGGGGATGGAATAGATGCCACCATCATATCGGGTAACCGCAGTTTTATTGACGGTTGGACAACATTCCGATCAGCGACCTTCGCTGTAAGTGGAAGAGGATTCATAGCGCGAGACATAACATTCGAGAATACTGCAGGAGCAGAGAAACATCAAGCAGTGGCACTTAGATCGGACTCCGATCTGTCGGTGTTTTTTAGATGTCGAATTAGAGGCTACCAAGACACGCTGTATACGCACACCATGCGCCAATTTTACCGCGAGTGCCAAATCAGTGGGACCGTTGATTTTCTTTTCGGCGATGCCACCGTCGTTTTTCAAAACTGCAGCATCCTGGCGAAGAAGGGTCTTCCCAACCAGAAAAACACCATCACTGCTCAGGGCCGGAAAGACCCAAACCAACCCACCGGTTTCTCCATTCAATTCTGCAACATATCTGCGGATTCAGACCTTAAACCCTCGGTTAACACTACTGCTACGTACCTTGGAAGACCATGGAAGGAATATTCCAGAACCATTATCATGCAATCATACATCAGCGATGCCATTAGGCCAGAAGGTTGGCTTGAGTGGAACGCCAATTTTGCATTGAATACACTATTCTACGCTGAGTTTATGAATTACGGACCGGGGGCGGGATTGGCCAAGCGTGTCAATTGGCCCGGTTACCACCGACTGAATCAAACCAGTGAAGCTACTAACTTCACCGTGGCTCAATTCATTGAAGGAAACCTGTGGCTGCCGTCCACGGGTGTCAAATACACTGCAGGACTGGGAGCTAATTAG

Protein sequence

MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPSEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN
BLAST of CsaV3_3G000050 vs. NCBI nr
Match: XP_004146188.2 (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus] >KGN55582.1 hypothetical protein Csa_3G000060 [Cucumis sativus])

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60
           MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA
Sbjct: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60

Query: 61  KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120
           KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST
Sbjct: 61  KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120

Query: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180
           IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD
Sbjct: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180

Query: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240
           LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS
Sbjct: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240

Query: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300
           EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY
Sbjct: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300

Query: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360
           LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT
Sbjct: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360

Query: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420
           AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV
Sbjct: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420

Query: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480
           FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP
Sbjct: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480

Query: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540
           WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR
Sbjct: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540

Query: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN
Sbjct: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576

BLAST of CsaV3_3G000050 vs. NCBI nr
Match: XP_008448427.1 (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo])

HSP 1 Score: 1047.7 bits (2708), Expect = 1.4e-302
Identity = 531/578 (91.87%), Postives = 539/578 (93.25%), Query Frame = 0

Query: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLK--FLSLTFQLDSSFTSPIFVLILLHHPIHTG 60
           MDF   P P     + +     P P   K  F  L   L   FT   FVLILLHHPIHTG
Sbjct: 1   MDFSDKPFPTKRECSIQINALSPQPPAFKVPFPHLPTGLFIYFTD--FVLILLHHPIHTG 60

Query: 61  QAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLL 120
           Q KGSSIIPILMAKLTTQ LQFCC+ ILLFPMLLPASSP DLVQTECLSVPSSQFSNSLL
Sbjct: 61  QGKGSSIIPILMAKLTTQILQFCCIFILLFPMLLPASSPADLVQTECLSVPSSQFSNSLL 120

Query: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHST 180
           STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAK+HST
Sbjct: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKDHST 180

Query: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQ 240
           GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGG+NQLTSQLYDLLSMVKSIPNQ
Sbjct: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGINQLTSQLYDLLSMVKSIPNQ 240

Query: 241 PSEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKG 300
           PSEFPSWLKSED+NLLQ+NDLAADATVAADGTGDFTNVMDAVLAAPD S RRYVIYIKKG
Sbjct: 241 PSEFPSWLKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDKSTRRYVIYIKKG 300

Query: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360
           VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE
Sbjct: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360

Query: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420
           NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT
Sbjct: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420

Query: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480
           VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG
Sbjct: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480

Query: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540
           RPWKEYSRTIIMQSYISDAIRPEGWLEWN NFALNTLFYAEFMNYGPGAGLAKRVNWPGY
Sbjct: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540

Query: 541 HRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           HRLNQTSEATNFTVAQFIEGN+WLPSTGVKYTAGLGAN
Sbjct: 541 HRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN 576

BLAST of CsaV3_3G000050 vs. NCBI nr
Match: XP_022949597.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita moschata])

HSP 1 Score: 887.9 bits (2293), Expect = 1.9e-254
Identity = 444/513 (86.55%), Postives = 477/513 (92.98%), Query Frame = 0

Query: 70  MAKLTTQTLQF-CCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVM 129
           MAKLT   L+F   +L++LFPM  PA+   DL QTECL VPSS+FSNS+LSTI VVRQVM
Sbjct: 1   MAKLTKTHLKFWLFMLLILFPM-APAA---DLEQTECLKVPSSEFSNSVLSTIGVVRQVM 60

Query: 130 AIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTW 189
            IFSPF+K LGD RLSTAISDCLDLLDSS+DQLSWSLSA QNPKAKN+STGDLSSDLKTW
Sbjct: 61  LIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTW 120

Query: 190 LSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQP-----SEFP 249
           LSAA VNPETCM+GFEGTNSI+KGLVS G+NQLTSQ+Y+LLSMVKSI NQP     SEFP
Sbjct: 121 LSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPVGKGESEFP 180

Query: 250 SWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLEN 309
           +WLK+E+Q+LLQ ND AADATVAADGTGDFT VM+AVLAAPD S+RRYVIY+KKGVY EN
Sbjct: 181 AWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFEN 240

Query: 310 VEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGA 369
           VEIKKKKWN+MMIGDGIDAT+ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG 
Sbjct: 241 VEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGP 300

Query: 370 EKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN 429
           EKHQAVALRSDSDLSVFFRCR+RGYQDTLYTHTMRQFYREC ISGTVDFLFGDATVVFQN
Sbjct: 301 EKHQAVALRSDSDLSVFFRCRMRGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQN 360

Query: 430 CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKE 489
           C+ILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+PSVN TATYLGRPWKE
Sbjct: 361 CTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKE 420

Query: 490 YSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ 549
           YSRTIIMQSY+S+AIRPEGWLEWN +FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ
Sbjct: 421 YSRTIIMQSYVSNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ 480

Query: 550 TSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           TSEATNFTV+QFIEGNLWLPSTGVK+T+G GAN
Sbjct: 481 TSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN 509

BLAST of CsaV3_3G000050 vs. NCBI nr
Match: XP_022971304.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita maxima])

HSP 1 Score: 887.9 bits (2293), Expect = 1.9e-254
Identity = 463/593 (78.08%), Postives = 499/593 (84.15%), Query Frame = 0

Query: 9   PQNV----NVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPI--------- 68
           P NV     V FKSTR+LPN     FL       +S T P    I    P+         
Sbjct: 16  PHNVIVKATVPFKSTRHLPNLRLRPFL-------NSLTFPPKYFIYSDSPVXXXXXXXXX 75

Query: 69  ------HTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVP 128
                       G    PILMAKLT   L+F   ++L+   L PA+   DL QTECL VP
Sbjct: 76  XXXXXXXXXXXXGHLNNPILMAKLTKTHLKFWLFMLLILFPLAPAA---DLEQTECLKVP 135

Query: 129 SSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQ 188
           SS+FSNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS+DQLSWSLSA Q
Sbjct: 136 SSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQ 195

Query: 189 NPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLL 248
           NPKAKN+STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G+NQLTSQ+Y+LL
Sbjct: 196 NPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLL 255

Query: 249 SMVKSIPNQP-----SEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAP 308
           SMVKSI NQP     S FP+WLKSE+Q+LLQ ND AADATVAADGTGDFT VM+AVLAAP
Sbjct: 256 SMVKSISNQPAGKGESGFPAWLKSEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAP 315

Query: 309 DNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFA 368
           D S+RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFIDGWTTFRSATFA
Sbjct: 316 DKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFA 375

Query: 369 VSG-RGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRE 428
           VSG RGFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTLYTHTMRQFYRE
Sbjct: 376 VSGRRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRE 435

Query: 429 CQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISAD 488
           C ISGTVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISAD
Sbjct: 436 CHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISAD 495

Query: 489 SDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNY 548
           SDL+PSVNTTATYLGRPWKEYSRTIIMQSY+S+AIRPEGWLEWN +FALNTLFYAEFMNY
Sbjct: 496 SDLQPSVNTTATYLGRPWKEYSRTIIMQSYMSNAIRPEGWLEWNGDFALNTLFYAEFMNY 555

Query: 549 GPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           GPGAGLAKR+NWPGYHRLNQTSEATNFTV+QFIEGNLWLPSTGVK+T+G GAN
Sbjct: 556 GPGAGLAKRINWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN 598

BLAST of CsaV3_3G000050 vs. NCBI nr
Match: XP_023539547.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 887.1 bits (2291), Expect = 3.2e-254
Identity = 445/516 (86.24%), Postives = 478/516 (92.64%), Query Frame = 0

Query: 67  PILMAKLTTQTLQF-CCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVR 126
           PILMAKLT   L+F   +L++LFPM  PA+   DL QTECL VPSS+FSNS+LSTI VVR
Sbjct: 112 PILMAKLTKTHLKFWLFMLLILFPM-APAA---DLEQTECLKVPSSEFSNSVLSTIGVVR 171

Query: 127 QVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDL 186
           QVM IFSPF+K LGD RLSTAISDCLDLLDSS+DQLSWSLSA QNPKAKN+STGDLSSDL
Sbjct: 172 QVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDL 231

Query: 187 KTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQP-----S 246
           KTWLSAA VNPETCM+GFEGTNSI+KGLVS G+NQLTSQ+Y+LLSMVKSI NQP     S
Sbjct: 232 KTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGES 291

Query: 247 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 306
           +FP+WLK+E+Q+LLQ ND AADATVAADGTGDFT VM+AVLAAPD S+RRYVIY+KKGVY
Sbjct: 292 KFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVY 351

Query: 307 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 366
            ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT
Sbjct: 352 FENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 411

Query: 367 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 426
           AG EKHQAVALRSDSDLSVFFRCR+ GYQDTLYTHTMRQFYREC ISGTVDFLFGDATVV
Sbjct: 412 AGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVV 471

Query: 427 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 486
           FQNC+ILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+P VN TATYLGRP
Sbjct: 472 FQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPLVNATATYLGRP 531

Query: 487 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 546
           WKEYSRTIIMQSYIS+AIRPEGWLEWN +FALNTLFYAEFMNYGPGAGLAKRVNWPGYHR
Sbjct: 532 WKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 591

Query: 547 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           LNQTSEATNFTV+QFIEGNLWLPSTGVK+T+G GAN
Sbjct: 592 LNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN 623

BLAST of CsaV3_3G000050 vs. TAIR10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 679.1 bits (1751), Expect = 2.4e-195
Identity = 323/498 (64.86%), Postives = 407/498 (81.73%), Query Frame = 0

Query: 94  ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
           A S  DLVQ ECL VP  +F+ +  + +D + + +AI S F K  G  R+S AI DC+DL
Sbjct: 28  AFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDL 87

Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
           LDS+A++LSW +SA+Q+P  K++STGD+ SDL+TW+SAA+ N +TC+DGFEGTN IIK +
Sbjct: 88  LDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKI 147

Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQP-----------------SEFPSWLKSEDQNLLQI 273
           V+GG++++ + + +LL+MV S P++P                 S+FPSW+K  D+ LLQ 
Sbjct: 148 VAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQT 207

Query: 274 NDL-AADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMM 333
           +++  ADA VAADGTG+FT + DAVLAAPD S +RYVI++K+GVY+ENVEIKKKKWN+MM
Sbjct: 208 DNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMM 267

Query: 334 IGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDS 393
           +GDGIDAT+I+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAG EKHQAVA+RSD+
Sbjct: 268 VGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDT 327

Query: 394 DLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQ 453
           DL VF+RC +RGYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQ
Sbjct: 328 DLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQ 387

Query: 454 KNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYIS 513
           KN+ITAQGRKDPN+PTGF+IQF NI+AD+DL  ++NTTATYLGRPWK YSRT+ MQ+Y+S
Sbjct: 388 KNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMS 447

Query: 514 DAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQF 573
           DAI P GWLEWN NFAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTV+Q 
Sbjct: 448 DAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQL 507

BLAST of CsaV3_3G000050 vs. TAIR10
Match: AT4G33220.1 (pectin methylesterase 44)

HSP 1 Score: 666.0 bits (1717), Expect = 2.1e-191
Identity = 325/521 (62.38%), Postives = 402/521 (77.16%), Query Frame = 0

Query: 81  CCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGD 140
           C    L+  ML    S  +  Q + L VP+S+F +S+ + + V+RQV +I S F+   GD
Sbjct: 3   CLKYFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 62

Query: 141 FRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCM 200
            RL  A+SDCLDLLD S+++L+WS SA++NPK K + TGD+ SD +TWLSAA+ N  TCM
Sbjct: 63  RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 122

Query: 201 DGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMV-------------------KSIP----- 260
           +GF+GT+ ++K LV+G ++QL S L +LL +V                   K+ P     
Sbjct: 123 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 182

Query: 261 ----NQPSEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYV 320
               ++  +FP W++ +D+ LL+ N    D +VA DGTG+FT +MDA+  APD S  R+V
Sbjct: 183 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 242

Query: 321 IYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIA 380
           IYIKKG+YLENVEIKKKKWN++M+GDGID T+ISGNRSFIDGWTTFRSATFAVSGRGF+A
Sbjct: 243 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLA 302

Query: 381 RDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDF 440
           RDITF+NTAG EKHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF
Sbjct: 303 RDITFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDF 362

Query: 441 LFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNT 500
           +FGD TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +NT
Sbjct: 363 IFGDGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNT 422

Query: 501 TATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKR 560
           T TYLGRPWK YSRT+ +++ +SD +RPEGWLEWNA+FAL+TLFY EFMNYGPG+GL+ R
Sbjct: 423 TRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSR 482

Query: 561 VNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           V WPGYH  N + +A NFTV+QFI+GNLWLPSTGV ++ GL
Sbjct: 483 VKWPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523

BLAST of CsaV3_3G000050 vs. TAIR10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 437.2 bits (1123), Expect = 1.6e-122
Identity = 221/452 (48.89%), Postives = 296/452 (65.49%), Query Frame = 0

Query: 142 RLSTAISDCLDLLDSSADQLSWSLSATQNPKA--------KNHSTGDLSSDLKTWLSAAV 201
           R   AI DC +L+  S  +L+WS+                 +H       +LKTWLSAA+
Sbjct: 100 REQVAIEDCKELVGFSVTELAWSMLEMNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAAM 159

Query: 202 VNPETCMDGFEGTNSIIKGLVSGG---VNQLTSQLYDLLSMVKSIPNQPSE------FPS 261
            N +TC++GFEGT    + L+ G    V QL S + D+ + + ++P + S        P 
Sbjct: 160 SNQDTCLEGFEGTERKYEELIKGSLRQVTQLVSNVLDMYTQLNALPFKASRNESVIASPE 219

Query: 262 WLKSEDQNLLQIND---LAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYL 321
           WL   D++L+  +D   +  +  VA DG G +  + +A+  AP++S +RYVIY+KKGVY 
Sbjct: 220 WLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYK 279

Query: 322 ENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTA 381
           EN+++KKKK N+M++GDGI  TII+G+R+F+ G TTFR+AT AVSGRGFIA+DITF NTA
Sbjct: 280 ENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTA 339

Query: 382 GAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVF 441
           G +  QAVALR DSD S F+RC + GYQDTLY H++RQFYR+C+I GT+DF+FG+   V 
Sbjct: 340 GPQNRQAVALRVDSDQSAFYRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVL 399

Query: 442 QNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPW 501
           QNC I  +  LP QK TITAQGRK PNQ TGF IQ   + A         T  TYLGRPW
Sbjct: 400 QNCKIYTRVPLPLQKVTITAQGRKSPNQNTGFVIQNSYVLA---------TQPTYLGRPW 459

Query: 502 KEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRL 561
           K YSRT+ M +Y+S  ++P GWLEW  NFAL+TL+Y E+ N GPG   + RV WPGYH +
Sbjct: 460 KLYSRTVYMNTYMSQLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIM 519

Query: 562 NQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           ++ + A +FTV  FI+G  WLP+TGV +TAGL
Sbjct: 520 DKRT-ALSFTVGSFIDGRRWLPATGVTFTAGL 541

BLAST of CsaV3_3G000050 vs. TAIR10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 429.1 bits (1102), Expect = 4.3e-120
Identity = 236/529 (44.61%), Postives = 323/529 (61.06%), Query Frame = 0

Query: 75  TQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPF 134
           T+  + C   ++ FP  L ASS  DL+    +++    FS++L S+  +    M      
Sbjct: 94  TRFPELCVDSLMDFPGSLAASSSKDLIHV-TVNMTLHHFSHALYSSASLSFVDMPP---- 153

Query: 135 SKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVV 194
                  R  +A   C++LLD S D LS +LS+  +  AK         D+ TWLSAA+ 
Sbjct: 154 -------RARSAYDSCVELLDDSVDALSRALSSVVSSSAK-------PQDVTTWLSAALT 213

Query: 195 NPETCMDGFEGTNSIIKGLVSGGV-NQLTSQLYDLLSMVKS------------------- 254
           N +TC +GF+G +        GGV + +T+ L +L  +V +                   
Sbjct: 214 NHDTCTEGFDGVD-------DGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPI 273

Query: 255 -------IPNQPSEFPSWLKSEDQNLLQ--INDLAADATVAADGTGDFTNVMDAVLAAPD 314
                  +  +  +FP W++ +++ +L+  ++ + AD  V+ DG G    + +A+  AP 
Sbjct: 274 QNRRLLGVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQ 333

Query: 315 NSIRRYVIYIKKGVYLE-NVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFA 374
           NS RR +IY+K G Y E N+++ +KK NLM +GDG   T+ISG +S  D  TTF +A+FA
Sbjct: 334 NSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFA 393

Query: 375 VSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYREC 434
            +G GFIARDITFEN AG  KHQAVALR  +D +V +RC I GYQDTLY H+ RQF+REC
Sbjct: 394 ATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFREC 453

Query: 435 QISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADS 494
            I GTVDF+FG+A VV QNCSI A+K +  QKNTITAQ RKDPNQ TG SI    + A S
Sbjct: 454 DIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAAS 513

Query: 495 DLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYG 554
           DL+ +  +T TYLGRPWK +SRT+ M SYI   +   GWLEWN  FAL+TL+Y E++N G
Sbjct: 514 DLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSG 573

Query: 555 PGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           PG+GL +RV+WPGY  +N T+EA  FTVA+FI G+ WLPSTGV + AGL
Sbjct: 574 PGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596

BLAST of CsaV3_3G000050 vs. TAIR10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F)

HSP 1 Score: 427.2 bits (1097), Expect = 1.6e-119
Identity = 226/456 (49.56%), Postives = 296/456 (64.91%), Query Frame = 0

Query: 142 RLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLS-SDLKTWLSAAVVNPETCM 201
           R+ +A   CL+LLD S D L+ +LS+          +GD S SD+ TWLS+A+ N +TC 
Sbjct: 136 RVRSAYDSCLELLDDSVDALTRALSSVV------VVSGDESHSDVMTWLSSAMTNHDTCT 195

Query: 202 DGF---EGTNSIIKGLVSGGVNQLTSQLYDLLSM----VKSIPNQP-------------S 261
           DGF   EG    +K  V G V  L+  + + L++    VK +   P              
Sbjct: 196 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETE 255

Query: 262 EFPSWLKSEDQNLL--QINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKG 321
           E P+WLK ED+ LL    + + AD TV+ DG+G F  + +A+  AP++S RR+VIY+K G
Sbjct: 256 ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAG 315

Query: 322 VY-LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITF 381
            Y  EN+++ +KK NLM IGDG   T+I+G +S  D  TTF +ATFA +G GFI RD+TF
Sbjct: 316 RYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTF 375

Query: 382 ENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDA 441
           EN AG  KHQAVALR   D +V +RC I GYQD LY H+ RQF+REC+I GTVDF+FG+A
Sbjct: 376 ENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNA 435

Query: 442 TVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYL 501
            V+ Q+C+I A+K +  QK TITAQ RKDPNQ TG SI  C + A  DL+ S  +  TYL
Sbjct: 436 AVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYL 495

Query: 502 GRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPG 561
           GRPWK YSR + M S + D I P GWLEWN  FAL++L+Y E+MN G G+G+ +RV WPG
Sbjct: 496 GRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPG 555

Query: 562 YHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           YH +  T EA+ FTVAQFI G+ WLPSTGV + +GL
Sbjct: 556 YHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of CsaV3_3G000050 vs. Swiss-Prot
Match: sp|Q43062|PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 3.9e-211
Identity = 364/533 (68.29%), Postives = 436/533 (81.80%), Query Frame = 0

Query: 46  FVLILLHHPIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTEC 105
           ++L+  H+P+  G  K   ++ +L A  ++  + F             + S  D +QT+C
Sbjct: 3   YLLMASHNPLPAG--KQLLLLVLLCAFFSSSFIPFA------------SCSITDDLQTQC 62

Query: 106 LSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSL 165
           L V +++F+ SL  TID V+QV +I S F+   GDFRL+ AISDCLDLLD SAD+L+WSL
Sbjct: 63  LKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADELNWSL 122

Query: 166 SATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQL 225
           SA+QN K KN+STG LSSDL+TWLSAA+VN +TC +GFEGTNSI++GL+S G+ Q+TS +
Sbjct: 123 SASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLV 182

Query: 226 YDLLSMVKSIPNQP---SEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLA 285
            +LL+ V    NQ     + PSW+K++D+ LLQ + ++ DA VA DGTG+FTNV DAVLA
Sbjct: 183 QELLTQVHPNSNQQGPNGQIPSWVKTKDRKLLQADGVSVDAIVAQDGTGNFTNVTDAVLA 242

Query: 286 APDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSAT 345
           APD S+RRYVIYIK+G Y ENVEIKKKKWNLMMIGDG+DATIISGNRSF+DGWTTFRSAT
Sbjct: 243 APDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSAT 302

Query: 346 FAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYR 405
           FAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVF+RC IRGYQDTLYTHTMRQFYR
Sbjct: 303 FAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYR 362

Query: 406 ECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISA 465
           +C+ISGTVDF+FGDATVVFQNC ILAKKGLPNQKN+ITAQGRKDPN+PTG SIQFCNI+A
Sbjct: 363 DCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITA 422

Query: 466 DSDLK-PSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFM 525
           DSDL+  SVN+T TYLGRPWK YSRT+IMQS++S+ IRPEGWLEWN +FALN+LFY E+M
Sbjct: 423 DSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYM 482

Query: 526 NYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLG 575
           NYGPGAGL  RV WPGY   N++++A N+TVAQFIEGNLWLPSTGVKYTA  G
Sbjct: 483 NYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFG 521

BLAST of CsaV3_3G000050 vs. Swiss-Prot
Match: sp|Q9LXK7|PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 4.3e-194
Identity = 323/498 (64.86%), Postives = 407/498 (81.73%), Query Frame = 0

Query: 94  ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
           A S  DLVQ ECL VP  +F+ +  + +D + + +AI S F K  G  R+S AI DC+DL
Sbjct: 28  AFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDL 87

Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
           LDS+A++LSW +SA+Q+P  K++STGD+ SDL+TW+SAA+ N +TC+DGFEGTN IIK +
Sbjct: 88  LDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKI 147

Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQP-----------------SEFPSWLKSEDQNLLQI 273
           V+GG++++ + + +LL+MV S P++P                 S+FPSW+K  D+ LLQ 
Sbjct: 148 VAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQT 207

Query: 274 NDL-AADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMM 333
           +++  ADA VAADGTG+FT + DAVLAAPD S +RYVI++K+GVY+ENVEIKKKKWN+MM
Sbjct: 208 DNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMM 267

Query: 334 IGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDS 393
           +GDGIDAT+I+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAG EKHQAVA+RSD+
Sbjct: 268 VGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDT 327

Query: 394 DLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQ 453
           DL VF+RC +RGYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQ
Sbjct: 328 DLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQ 387

Query: 454 KNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYIS 513
           KN+ITAQGRKDPN+PTGF+IQF NI+AD+DL  ++NTTATYLGRPWK YSRT+ MQ+Y+S
Sbjct: 388 KNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMS 447

Query: 514 DAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQF 573
           DAI P GWLEWN NFAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTV+Q 
Sbjct: 448 DAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQL 507

BLAST of CsaV3_3G000050 vs. Swiss-Prot
Match: sp|Q9SMY7|PME44_ARATH (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 666.0 bits (1717), Expect = 3.8e-190
Identity = 325/521 (62.38%), Postives = 402/521 (77.16%), Query Frame = 0

Query: 81  CCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGD 140
           C    L+  ML    S  +  Q + L VP+S+F +S+ + + V+RQV +I S F+   GD
Sbjct: 3   CLKYFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 62

Query: 141 FRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCM 200
            RL  A+SDCLDLLD S+++L+WS SA++NPK K + TGD+ SD +TWLSAA+ N  TCM
Sbjct: 63  RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 122

Query: 201 DGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMV-------------------KSIP----- 260
           +GF+GT+ ++K LV+G ++QL S L +LL +V                   K+ P     
Sbjct: 123 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 182

Query: 261 ----NQPSEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYV 320
               ++  +FP W++ +D+ LL+ N    D +VA DGTG+FT +MDA+  APD S  R+V
Sbjct: 183 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 242

Query: 321 IYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIA 380
           IYIKKG+YLENVEIKKKKWN++M+GDGID T+ISGNRSFIDGWTTFRSATFAVSGRGF+A
Sbjct: 243 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLA 302

Query: 381 RDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDF 440
           RDITF+NTAG EKHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF
Sbjct: 303 RDITFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDF 362

Query: 441 LFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNT 500
           +FGD TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +NT
Sbjct: 363 IFGDGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNT 422

Query: 501 TATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKR 560
           T TYLGRPWK YSRT+ +++ +SD +RPEGWLEWNA+FAL+TLFY EFMNYGPG+GL+ R
Sbjct: 423 TRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSR 482

Query: 561 VNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           V WPGYH  N + +A NFTV+QFI+GNLWLPSTGV ++ GL
Sbjct: 483 VKWPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523

BLAST of CsaV3_3G000050 vs. Swiss-Prot
Match: sp|Q96575|PME22_SOLLC (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 6.6e-126
Identity = 232/489 (47.44%), Postives = 318/489 (65.03%), Query Frame = 0

Query: 99  DLVQTECLSVPSSQFSNSLLSTIDVVRQV---MAIFSPFSKLLGDFRLSTAISDCLDLLD 158
           DL+  E ++  S   S  +   ++ V Q+   + + S     + D R   A++DCL+LLD
Sbjct: 69  DLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQEGALTDCLELLD 128

Query: 159 SSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVS 218
            S D +S S++A        H      ++ ++WLS  + N  TC+D  +   S  K +++
Sbjct: 129 QSVDLVSDSIAAIDKRTHSEH------ANAQSWLSGVLTNHVTCLDELD---SFTKAMIN 188

Query: 219 G-GVNQLTSQLYDLLSMVKSI--PNQP------SEFPSWLKSEDQNLLQIN--DLAADAT 278
           G  +++L S+    L+M+ S+  PN         + PSW+ S D+ L++ +  D+ A+A 
Sbjct: 189 GTNLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGANAV 248

Query: 279 VAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATI 338
           VA DGTG +  + +AV AAPD S  RYVIY+K+G+Y ENVE+  +K  LM++GDG+ ATI
Sbjct: 249 VAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMHATI 308

Query: 339 ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCR 398
           I+GN + +DG TTF SAT A  G+GFI +DI  +NTAG  KHQAVALR  +D SV  RCR
Sbjct: 309 ITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVINRCR 368

Query: 399 IRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGR 458
           I  YQDTLY H+ RQFYR+  ++GT+DF+FG+A VVFQ C ++A+K    Q+N +TAQGR
Sbjct: 369 IDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQQNMVTAQGR 428

Query: 459 KDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWL 518
            DPNQ TG SIQFCNI A SDL+P +    TYLGRPWK+YSRT++M+SY+   I P GW 
Sbjct: 429 TDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPAGWA 488

Query: 519 EWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPS 574
           EW+ +FAL TL+Y EFMN GPGAG +KRV WPGYH +   +EA  FTVA+ I+G  WL S
Sbjct: 489 EWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSWLRS 548

BLAST of CsaV3_3G000050 vs. Swiss-Prot
Match: sp|P14280|PME1_SOLLC (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 447.2 bits (1149), Expect = 2.8e-124
Identity = 244/548 (44.53%), Postives = 332/548 (60.58%), Query Frame = 0

Query: 40  SFTSPIFVLILLHHPIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPD 99
           SF   +FV + L  P    + K S++        T Q  Q C             S   D
Sbjct: 25  SFVITLFVALFLVAPYQV-EIKHSNLCK------TAQDSQLC------------LSYVSD 84

Query: 100 LVQTECLSVPSSQFSNSLLSTIDVVRQV---MAIFSPFSKLLGDFRLSTAISDCLDLLDS 159
           L+  E ++  S   S  +   ++ V Q+   + +       + D R   A++DCL+LLD 
Sbjct: 85  LISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHGALTDCLELLDQ 144

Query: 160 SADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSG 219
           S D  S S++A        H      ++ ++WLS  + N  TC+D  +   S  K +++G
Sbjct: 145 SVDFASDSIAAIDKRSRSEH------ANAQSWLSGVLTNHVTCLDELD---SFTKAMING 204

Query: 220 -GVNQLTSQLYDLLSMVKSIPNQPSE--------FPSWLKSEDQNLLQIN--DLAADATV 279
             + +L S+    L+M+ S+  Q  +         PSW+ S D+ L++ +  D+ A+A V
Sbjct: 205 TNLEELISRAKVALAMLASLTTQDEDVFMTVLGKMPSWVSSMDRKLMESSGKDIIANAVV 264

Query: 280 AADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATII 339
           A DGTGD+  + +AV AAPD S  RYVIY+K+G Y ENVE+   K NLM++GDG+ AT I
Sbjct: 265 AQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMIVGDGMYATTI 324

Query: 340 SGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRI 399
           +G+ + +DG TTFRSAT A  G+GFI +DI  +NTAG  K QAVALR  +D+SV  RCRI
Sbjct: 325 TGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGADMSVINRCRI 384

Query: 400 RGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRK 459
             YQDTLY H+ RQFYR+  ++GTVDF+FG+A VVFQ C ++A+K    Q+N +TAQGR 
Sbjct: 385 DAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQQNMVTAQGRT 444

Query: 460 DPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLE 519
           DPNQ TG SIQFCNI A SDL+P +    TYLGRPWKEYSRT++M+SY+   I P GW E
Sbjct: 445 DPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWAE 504

Query: 520 WNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPST 574
           W+ +FAL TL+Y EFMN GPGAG +KRV WPGYH +   ++A  FTVA+ I+G  WL ST
Sbjct: 505 WDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRST 544

BLAST of CsaV3_3G000050 vs. TrEMBL
Match: tr|A0A0A0L4S0|A0A0A0L4S0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G000060 PE=3 SV=1)

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60
           MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA
Sbjct: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60

Query: 61  KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120
           KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST
Sbjct: 61  KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120

Query: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180
           IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD
Sbjct: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180

Query: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240
           LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS
Sbjct: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240

Query: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300
           EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY
Sbjct: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300

Query: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360
           LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT
Sbjct: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360

Query: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420
           AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV
Sbjct: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420

Query: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480
           FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP
Sbjct: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480

Query: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540
           WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR
Sbjct: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540

Query: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN
Sbjct: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576

BLAST of CsaV3_3G000050 vs. TrEMBL
Match: tr|A0A1S3BKA0|A0A1S3BKA0_CUCME (pectinesterase/pectinesterase inhibitor PPE8B-like OS=Cucumis melo OX=3656 GN=LOC103490630 PE=3 SV=1)

HSP 1 Score: 1047.7 bits (2708), Expect = 9.4e-303
Identity = 531/578 (91.87%), Postives = 539/578 (93.25%), Query Frame = 0

Query: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLK--FLSLTFQLDSSFTSPIFVLILLHHPIHTG 60
           MDF   P P     + +     P P   K  F  L   L   FT   FVLILLHHPIHTG
Sbjct: 1   MDFSDKPFPTKRECSIQINALSPQPPAFKVPFPHLPTGLFIYFTD--FVLILLHHPIHTG 60

Query: 61  QAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLL 120
           Q KGSSIIPILMAKLTTQ LQFCC+ ILLFPMLLPASSP DLVQTECLSVPSSQFSNSLL
Sbjct: 61  QGKGSSIIPILMAKLTTQILQFCCIFILLFPMLLPASSPADLVQTECLSVPSSQFSNSLL 120

Query: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHST 180
           STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAK+HST
Sbjct: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKDHST 180

Query: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQ 240
           GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGG+NQLTSQLYDLLSMVKSIPNQ
Sbjct: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGINQLTSQLYDLLSMVKSIPNQ 240

Query: 241 PSEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKG 300
           PSEFPSWLKSED+NLLQ+NDLAADATVAADGTGDFTNVMDAVLAAPD S RRYVIYIKKG
Sbjct: 241 PSEFPSWLKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDKSTRRYVIYIKKG 300

Query: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360
           VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE
Sbjct: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360

Query: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420
           NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT
Sbjct: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420

Query: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480
           VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG
Sbjct: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480

Query: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540
           RPWKEYSRTIIMQSYISDAIRPEGWLEWN NFALNTLFYAEFMNYGPGAGLAKRVNWPGY
Sbjct: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540

Query: 541 HRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           HRLNQTSEATNFTVAQFIEGN+WLPSTGVKYTAGLGAN
Sbjct: 541 HRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN 576

BLAST of CsaV3_3G000050 vs. TrEMBL
Match: tr|A0A2N9H526|A0A2N9H526_FAGSY (Pectinesterase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34536 PE=3 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 3.5e-217
Identity = 365/484 (75.41%), Postives = 423/484 (87.40%), Query Frame = 0

Query: 99  DLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSA 158
           DL+Q+ECL VPSS+F NS+ +TID V+ V +I SPF+ +  D R+S AISDCLDLLD SA
Sbjct: 24  DLLQSECLKVPSSEFLNSVKNTIDAVQNVASIISPFANVFQDLRISNAISDCLDLLDFSA 83

Query: 159 DQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGV 218
           D+LSWSLSATQNPK KN+STGDLSSDL+TWLSAA+VN +TC++GF+GTNSI++GLV+GG+
Sbjct: 84  DELSWSLSATQNPKGKNNSTGDLSSDLRTWLSAALVNQDTCIEGFDGTNSIVQGLVTGGL 143

Query: 219 NQLTSQLYDLLSMVKSIPNQP-------SEFPSWLKSEDQNLLQINDLAADATVAADGTG 278
           NQ+TS +  LL+MV  + + P        +FPSW+K ED+ L+Q N +  DA VAADG+G
Sbjct: 144 NQVTSLVQQLLNMVHPVSDNPGRRLESNDQFPSWMKLEDRKLVQANGVTVDAIVAADGSG 203

Query: 279 DFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSF 338
           +FT VMDA+LAAPD S+RRYVIYIKKGVY E VEIKKKKWNLMMIGDG++ATIISGNR++
Sbjct: 204 NFTKVMDAILAAPDFSMRRYVIYIKKGVYNEYVEIKKKKWNLMMIGDGMNATIISGNRNY 263

Query: 339 IDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDT 398
           +DGWTTF +ATFAVSGRGFIARDITFEN+AG EKHQAVALRSDSDLSVFFRC IRGYQDT
Sbjct: 264 VDGWTTFHTATFAVSGRGFIARDITFENSAGPEKHQAVALRSDSDLSVFFRCGIRGYQDT 323

Query: 399 LYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPT 458
           LYTHTMRQFYREC+ISGT+DF+FGD +VVFQNC ILA+ GLPNQKNTITAQGRKDPNQPT
Sbjct: 324 LYTHTMRQFYRECRISGTIDFIFGDGSVVFQNCQILARMGLPNQKNTITAQGRKDPNQPT 383

Query: 459 GFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFA 518
           GFSIQFCNIS DSDL  SVN+T+TYLGRPWK YSRTIIMQSYIS+A RPEGWLEWN +  
Sbjct: 384 GFSIQFCNISVDSDLATSVNSTSTYLGRPWKPYSRTIIMQSYISNATRPEGWLEWNGSLY 443

Query: 519 LNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTA 576
           L+TLFY E+MNYGPG+GL  RV WPGYH LNQ+S+ TNFTVAQFIEGNLWLPSTGVKYTA
Sbjct: 444 LDTLFYGEYMNYGPGSGLGNRVKWPGYHALNQSSQVTNFTVAQFIEGNLWLPSTGVKYTA 503

BLAST of CsaV3_3G000050 vs. TrEMBL
Match: tr|A0A2P4HW96|A0A2P4HW96_QUESU (Pectinesterase OS=Quercus suber OX=58331 GN=CFP56_61012 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 5.8e-212
Identity = 359/492 (72.97%), Postives = 416/492 (84.55%), Query Frame = 0

Query: 94  ASSPPDLVQ-TECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLD 153
           ASS  D +Q +ECL VP+S+F +S+ +TID V+ V++I SPF+KL GD R+S AISDCLD
Sbjct: 15  ASSGKDFLQYSECLKVPTSEFLSSVKTTIDAVQSVVSIISPFAKLFGDLRVSNAISDCLD 74

Query: 154 LLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKG 213
           LLD SAD+L+WSLSATQNPK   + TGDLSSDL+TWLSAA+VN +TC++GFEGTNSI+ G
Sbjct: 75  LLDFSADELAWSLSATQNPKGNQNGTGDLSSDLRTWLSAALVNQDTCIEGFEGTNSIVAG 134

Query: 214 LVSGGVNQLTSQLYDLLSMVKSIPNQP----------SEFPSWLKSEDQNLLQINDLAAD 273
           L+ GG+NQ+TS +  LL MV  + N P           +FPSW+KSED+ LLQ N    D
Sbjct: 135 LIDGGLNQVTSLVQQLLDMVHPVLNNPRGRRLSGNANEQFPSWVKSEDRKLLQANGFTVD 194

Query: 274 ATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDA 333
             VAADG+G++T +MDA+  APD S+RRYVIYIKKGVY E VEIKKKKWNLMMIGDG+DA
Sbjct: 195 VIVAADGSGNYTKMMDAIKDAPDYSMRRYVIYIKKGVYNETVEIKKKKWNLMMIGDGMDA 254

Query: 334 TIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFR 393
           TII+GN +++DGWTTF SATFAVSG+GFIARD+TFENTAG EKHQAVALRSDSDLSVFFR
Sbjct: 255 TIITGNHNYVDGWTTFHSATFAVSGKGFIARDMTFENTAGPEKHQAVALRSDSDLSVFFR 314

Query: 394 CRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQ 453
           C IRGYQDTLYTHTMRQFYREC+ISGT+DF+FGD +VVFQNC IL +KGLPNQKNTITAQ
Sbjct: 315 CGIRGYQDTLYTHTMRQFYRECRISGTIDFIFGDGSVVFQNCDILVRKGLPNQKNTITAQ 374

Query: 454 GRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEG 513
           GRKDP++PTGFSIQFCNISADSDL PSVN+T TYLGRPWK YSRTIIMQSYIS+A RPEG
Sbjct: 375 GRKDPSEPTGFSIQFCNISADSDLVPSVNSTYTYLGRPWKAYSRTIIMQSYISNATRPEG 434

Query: 514 WLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWL 573
           WLEWN    L+TL+Y E+MN GPGAGL+ RV WPGYH LNQ+S+  NFTVAQFIEGNLWL
Sbjct: 435 WLEWNGTLHLDTLYYGEYMNNGPGAGLSNRVKWPGYHALNQSSQVNNFTVAQFIEGNLWL 494

Query: 574 PSTGVKYTAGLG 575
           PSTGVKYTAGLG
Sbjct: 495 PSTGVKYTAGLG 506

BLAST of CsaV3_3G000050 vs. TrEMBL
Match: tr|A0A061GL47|A0A061GL47_THECC (Pectinesterase OS=Theobroma cacao OX=3641 GN=TCM_037443 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 5.8e-212
Identity = 369/539 (68.46%), Postives = 435/539 (80.71%), Query Frame = 0

Query: 65  IIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVV 124
           ++P  M   ++ +L    + + L       SS  D +  ECL+VP+++F  SL +TID V
Sbjct: 1   MLPKNMPNFSSHSLLVLLVALYLCCTSYATSSSDDFLGAECLNVPAAEFVGSLRTTIDAV 60

Query: 125 RQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSD 184
           R+V +I S      GDFRLS AISDCLDLLD SAD+LSW+LSA+QNPKAKN+STGDLSS+
Sbjct: 61  RKVTSIVSQLGGFFGDFRLSNAISDCLDLLDFSADELSWTLSASQNPKAKNNSTGDLSSN 120

Query: 185 LKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMV------------ 244
           L+TWLSAA+ N  TC+DGFEGT+S+IK +V+G +NQ+TS + DLL+MV            
Sbjct: 121 LRTWLSAAMANQATCIDGFEGTSSVIKPVVAGSLNQVTSLVSDLLTMVHPGPSSKSNXXX 180

Query: 245 ------------------KSIPNQPSEFPSWLKSEDQNLLQINDLAADATVAADGTGDFT 304
                             + + N  ++FPSW+K ED+ LL++N ++AD  VAADGTG+FT
Sbjct: 181 XXXXXXXXXXXXXXXXPGQKLANNKNQFPSWVKREDRKLLEVNGVSADVVVAADGTGNFT 240

Query: 305 NVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDG 364
           ++MDAV A PD S+ RYVIYIKKG+Y ENVEIKKKKWNLMM+GDG+D T+ISGNRSFIDG
Sbjct: 241 SIMDAVAAVPDYSMSRYVIYIKKGLYKENVEIKKKKWNLMMVGDGMDVTVISGNRSFIDG 300

Query: 365 WTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYT 424
           WTTFRSATFAVSGRGFIARDITFENTAG +KHQAVALRSDSDLSVFFRC IRGYQD+LYT
Sbjct: 301 WTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCAIRGYQDSLYT 360

Query: 425 HTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFS 484
           HTMRQF+REC+I+GTVDF+FGDATVVFQ C ILAK+GLPNQKNTITAQGRKDPNQPTGFS
Sbjct: 361 HTMRQFFRECKITGTVDFIFGDATVVFQYCQILAKQGLPNQKNTITAQGRKDPNQPTGFS 420

Query: 485 IQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNT 544
           IQFCNISAD+DL P VN+TATYLGRPWK YSRTIIMQSYIS+AIRPEGWLEWN +FAL+T
Sbjct: 421 IQFCNISADTDLLPFVNSTATYLGRPWKLYSRTIIMQSYISNAIRPEGWLEWNQDFALDT 480

Query: 545 LFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           LFYAEF+N+GPGAGL  RV WPGYH LN +++A NFTVAQFIEGNLWLPSTGVKYTAGL
Sbjct: 481 LFYAEFLNFGPGAGLGSRVKWPGYHALNNSAQAANFTVAQFIEGNLWLPSTGVKYTAGL 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146188.20.0e+00100.00PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus] ... [more]
XP_008448427.11.4e-30291.87PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo][more]
XP_022949597.11.9e-25486.55pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita moschata][more]
XP_022971304.11.9e-25478.08pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita maxima][more]
XP_023539547.13.2e-25486.24pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G43270.12.4e-19564.86Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G33220.12.1e-19162.38pectin methylesterase 44[more]
AT3G05620.11.6e-12248.89Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G49220.14.3e-12044.61Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G53370.11.6e-11949.56pectin methylesterase PCR fragment F[more]
Match NameE-valueIdentityDescription
sp|Q43062|PME_PRUPE3.9e-21168.29Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=... [more]
sp|Q9LXK7|PME32_ARATH4.3e-19464.86Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3... [more]
sp|Q9SMY7|PME44_ARATH3.8e-19062.38Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3... [more]
sp|Q96575|PME22_SOLLC6.6e-12647.44Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1[more]
sp|P14280|PME1_SOLLC2.8e-12444.53Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5[more]
Match NameE-valueIdentityDescription
tr|A0A0A0L4S0|A0A0A0L4S0_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G000060 PE=3 SV=1[more]
tr|A0A1S3BKA0|A0A1S3BKA0_CUCME9.4e-30391.87pectinesterase/pectinesterase inhibitor PPE8B-like OS=Cucumis melo OX=3656 GN=LO... [more]
tr|A0A2N9H526|A0A2N9H526_FAGSY3.5e-21775.41Pectinesterase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34536 PE=3 SV=1[more]
tr|A0A2P4HW96|A0A2P4HW96_QUESU5.8e-21272.97Pectinesterase OS=Quercus suber OX=58331 GN=CFP56_61012 PE=3 SV=1[more]
tr|A0A061GL47|A0A061GL47_THECC5.8e-21268.46Pectinesterase OS=Theobroma cacao OX=3641 GN=TCM_037443 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
IPR012334Pectin_lyas_fold
IPR035513Invertase/methylesterase_inhib
IPR006501Pectinesterase_inhib_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0050829 defense response to Gram-negative bacterium
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_3G000050.1CsaV3_3G000050.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 96..235
e-value: 9.3E-12
score: 55.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 142..223
e-value: 2.5E-14
score: 53.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3DG3DSA:1.20.140.40coord: 118..236
e-value: 6.7E-7
score: 31.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILYSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 117..235
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 259..575
e-value: 2.4E-133
score: 446.3
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 262..559
e-value: 9.3E-149
score: 494.0
NoneNo IPR availablePANTHERPTHR31707:SF10PECTINESTERASE/PECTINESTERASE INHIBITOR 32-RELATEDcoord: 83..573
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 83..573
NoneNo IPR availableCDDcd15799PMEI-like_4coord: 94..236
e-value: 5.75265E-40
score: 141.779
IPR011050Pectin lyase fold/virulence factorSUPERFAMILYSSF51126Pectin lyase-likecoord: 260..572