BLAST of Cucsa.323330 vs. Swiss-Prot
Match:
PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1)
HSP 1 Score: 738.4 bits (1905), Expect = 5.9e-212
Identity = 364/533 (68.29%), Postives = 436/533 (81.80%), Query Frame = 1
Query: 46 FVLILLHHPIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTEC 105
++L+ H+P+ G K ++ +L A ++ + F + S D +QT+C
Sbjct: 3 YLLMASHNPLPAG--KQLLLLVLLCAFFSSSFIPFA------------SCSITDDLQTQC 62
Query: 106 LSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSL 165
L V +++F+ SL TID V+QV +I S F+ GDFRL+ AISDCLDLLD SAD+L+WSL
Sbjct: 63 LKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADELNWSL 122
Query: 166 SATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQL 225
SA+QN K KN+STG LSSDL+TWLSAA+VN +TC +GFEGTNSI++GL+S G+ Q+TS +
Sbjct: 123 SASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLV 182
Query: 226 YDLLSMVKSIPNQPS---EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLA 285
+LL+ V NQ + PSW+K++D+ LLQ + ++ DA VA DGTG+FTNV DAVLA
Sbjct: 183 QELLTQVHPNSNQQGPNGQIPSWVKTKDRKLLQADGVSVDAIVAQDGTGNFTNVTDAVLA 242
Query: 286 APDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSAT 345
APD S+RRYVIYIK+G Y ENVEIKKKKWNLMMIGDG+DATIISGNRSF+DGWTTFRSAT
Sbjct: 243 APDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSAT 302
Query: 346 FAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYR 405
FAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVF+RC IRGYQDTLYTHTMRQFYR
Sbjct: 303 FAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYR 362
Query: 406 ECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISA 465
+C+ISGTVDF+FGDATVVFQNC ILAKKGLPNQKN+ITAQGRKDPN+PTG SIQFCNI+A
Sbjct: 363 DCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITA 422
Query: 466 DSDLK-PSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFM 525
DSDL+ SVN+T TYLGRPWK YSRT+IMQS++S+ IRPEGWLEWN +FALN+LFY E+M
Sbjct: 423 DSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYM 482
Query: 526 NYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLG 575
NYGPGAGL RV WPGY N++++A N+TVAQFIEGNLWLPSTGVKYTA G
Sbjct: 483 NYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFG 521
BLAST of Cucsa.323330 vs. Swiss-Prot
Match:
PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=PME32 PE=2 SV=1)
HSP 1 Score: 679.5 bits (1752), Expect = 3.3e-194
Identity = 323/498 (64.86%), Postives = 407/498 (81.73%), Query Frame = 1
Query: 94 ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
A S DLVQ ECL VP +F+ + + +D + + +AI S F K G R+S AI DC+DL
Sbjct: 28 AFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDL 87
Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
LDS+A++LSW +SA+Q+P K++STGD+ SDL+TW+SAA+ N +TC+DGFEGTN IIK +
Sbjct: 88 LDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKI 147
Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQP-----------------SEFPSWLKSEDQNLLQI 273
V+GG++++ + + +LL+MV S P++P S+FPSW+K D+ LLQ
Sbjct: 148 VAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQT 207
Query: 274 NDLA-ADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMM 333
+++ ADA VAADGTG+FT + DAVLAAPD S +RYVI++K+GVY+ENVEIKKKKWN+MM
Sbjct: 208 DNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMM 267
Query: 334 IGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDS 393
+GDGIDAT+I+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAG EKHQAVA+RSD+
Sbjct: 268 VGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDT 327
Query: 394 DLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQ 453
DL VF+RC +RGYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQ
Sbjct: 328 DLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQ 387
Query: 454 KNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYIS 513
KN+ITAQGRKDPN+PTGF+IQF NI+AD+DL ++NTTATYLGRPWK YSRT+ MQ+Y+S
Sbjct: 388 KNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMS 447
Query: 514 DAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQF 573
DAI P GWLEWN NFAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTV+Q
Sbjct: 448 DAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQL 507
BLAST of Cucsa.323330 vs. Swiss-Prot
Match:
PME44_ARATH (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=PME44 PE=2 SV=2)
HSP 1 Score: 670.2 bits (1728), Expect = 2.0e-191
Identity = 325/521 (62.38%), Postives = 402/521 (77.16%), Query Frame = 1
Query: 81 CCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGD 140
C L+ ML S + Q + L VP+S+F +S+ + + V+RQV +I S F+ GD
Sbjct: 3 CLKYFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 62
Query: 141 FRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCM 200
RL A+SDCLDLLD S+++L+WS SA++NPK K + TGD+ SD +TWLSAA+ N TCM
Sbjct: 63 RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 122
Query: 201 DGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMV-------------------KSIP----- 260
+GF+GT+ ++K LV+G ++QL S L +LL +V K+ P
Sbjct: 123 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 182
Query: 261 ----NQPSEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYV 320
++ +FP W++ +D+ LL+ N D +VA DGTG+FT +MDA+ APD S R+V
Sbjct: 183 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 242
Query: 321 IYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIA 380
IYIKKG+YLENVEIKKKKWN++M+GDGID T+ISGNRSFIDGWTTFRSATFAVSGRGF+A
Sbjct: 243 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLA 302
Query: 381 RDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDF 440
RDITF+NTAG EKHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF
Sbjct: 303 RDITFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDF 362
Query: 441 LFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNT 500
+FGD TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +NT
Sbjct: 363 IFGDGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNT 422
Query: 501 TATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKR 560
T TYLGRPWK YSRT+ +++ +SD +RPEGWLEWNA+FAL+TLFY EFMNYGPG+GL+ R
Sbjct: 423 TRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSR 482
Query: 561 VNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
V WPGYH N + +A NFTV+QFI+GNLWLPSTGV ++ GL
Sbjct: 483 VKWPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523
BLAST of Cucsa.323330 vs. Swiss-Prot
Match:
PME22_SOLLC (Pectinesterase 2.2 OS=Solanum lycopersicum GN=PME2.2 PE=3 SV=1)
HSP 1 Score: 452.6 bits (1163), Expect = 6.5e-126
Identity = 232/489 (47.44%), Postives = 317/489 (64.83%), Query Frame = 1
Query: 99 DLVQTECLSVPSSQFSNSLLSTIDVVRQV---MAIFSPFSKLLGDFRLSTAISDCLDLLD 158
DL+ E ++ S S + ++ V Q+ + + S + D R A++DCL+LLD
Sbjct: 69 DLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQEGALTDCLELLD 128
Query: 159 SSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVS 218
S D +S S++A H+ ++WLS + N TC+D + S K +++
Sbjct: 129 QSVDLVSDSIAAIDKRTHSEHANA------QSWLSGVLTNHVTCLDELD---SFTKAMIN 188
Query: 219 G-GVNQLTSQLYDLLSMVKSI--PNQP------SEFPSWLKSEDQNLLQIN--DLAADAT 278
G +++L S+ L+M+ S+ PN + PSW+ S D+ L++ + D+ A+A
Sbjct: 189 GTNLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGANAV 248
Query: 279 VAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATI 338
VA DGTG + + +AV AAPD S RYVIY+K+G+Y ENVE+ +K LM++GDG+ ATI
Sbjct: 249 VAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMHATI 308
Query: 339 ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCR 398
I+GN + +DG TTF SAT A G+GFI +DI +NTAG KHQAVALR +D SV RCR
Sbjct: 309 ITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVINRCR 368
Query: 399 IRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGR 458
I YQDTLY H+ RQFYR+ ++GT+DF+FG+A VVFQ C ++A+K Q+N +TAQGR
Sbjct: 369 IDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQQNMVTAQGR 428
Query: 459 KDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWL 518
DPNQ TG SIQFCNI A SDL+P + TYLGRPWK+YSRT++M+SY+ I P GW
Sbjct: 429 TDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPAGWA 488
Query: 519 EWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPS 574
EW+ +FAL TL+Y EFMN GPGAG +KRV WPGYH + +EA FTVA+ I+G WL S
Sbjct: 489 EWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSWLRS 548
BLAST of Cucsa.323330 vs. Swiss-Prot
Match:
PME1_SOLLC (Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9 PE=1 SV=5)
HSP 1 Score: 448.0 bits (1151), Expect = 1.6e-124
Identity = 243/548 (44.34%), Postives = 331/548 (60.40%), Query Frame = 1
Query: 40 SFTSPIFVLILLHHPIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPD 99
SF +FV + L P + K S++ T Q Q C + D
Sbjct: 25 SFVITLFVALFLVAPYQV-EIKHSNLCK------TAQDSQLCLSYV------------SD 84
Query: 100 LVQTECLSVPSSQFSNSLLSTIDVVRQV---MAIFSPFSKLLGDFRLSTAISDCLDLLDS 159
L+ E ++ S S + ++ V Q+ + + + D R A++DCL+LLD
Sbjct: 85 LISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHGALTDCLELLDQ 144
Query: 160 SADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSG 219
S D S S++A H+ ++WLS + N TC+D + S K +++G
Sbjct: 145 SVDFASDSIAAIDKRSRSEHANA------QSWLSGVLTNHVTCLDELD---SFTKAMING 204
Query: 220 -GVNQLTSQLYDLLSMVKSIPNQPSE--------FPSWLKSEDQNLLQIN--DLAADATV 279
+ +L S+ L+M+ S+ Q + PSW+ S D+ L++ + D+ A+A V
Sbjct: 205 TNLEELISRAKVALAMLASLTTQDEDVFMTVLGKMPSWVSSMDRKLMESSGKDIIANAVV 264
Query: 280 AADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATII 339
A DGTGD+ + +AV AAPD S RYVIY+K+G Y ENVE+ K NLM++GDG+ AT I
Sbjct: 265 AQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMIVGDGMYATTI 324
Query: 340 SGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRI 399
+G+ + +DG TTFRSAT A G+GFI +DI +NTAG K QAVALR +D+SV RCRI
Sbjct: 325 TGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGADMSVINRCRI 384
Query: 400 RGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRK 459
YQDTLY H+ RQFYR+ ++GTVDF+FG+A VVFQ C ++A+K Q+N +TAQGR
Sbjct: 385 DAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQQNMVTAQGRT 444
Query: 460 DPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLE 519
DPNQ TG SIQFCNI A SDL+P + TYLGRPWKEYSRT++M+SY+ I P GW E
Sbjct: 445 DPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWAE 504
Query: 520 WNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPST 574
W+ +FAL TL+Y EFMN GPGAG +KRV WPGYH + ++A FTVA+ I+G WL ST
Sbjct: 505 WDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRST 544
BLAST of Cucsa.323330 vs. TrEMBL
Match:
A0A0A0L4S0_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G000060 PE=4 SV=1)
HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 1
Query: 1 MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60
MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA
Sbjct: 1 MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60
Query: 61 KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120
KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST
Sbjct: 61 KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120
Query: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180
IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD
Sbjct: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180
Query: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240
LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS
Sbjct: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240
Query: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300
EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY
Sbjct: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300
Query: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360
LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT
Sbjct: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360
Query: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420
AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV
Sbjct: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420
Query: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480
FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP
Sbjct: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480
Query: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540
WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR
Sbjct: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540
Query: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN
Sbjct: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576
BLAST of Cucsa.323330 vs. TrEMBL
Match:
R9QPU3_GOSHI (Pectinesterase OS=Gossypium hirsutum PE=2 SV=1)
HSP 1 Score: 737.6 bits (1903), Expect = 1.1e-209
Identity = 356/501 (71.06%), Postives = 418/501 (83.43%), Query Frame = 1
Query: 94 ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
+SS + ++TECL VP+++F SL +TID +R+ ++ S F DFRLS AISDCLDL
Sbjct: 24 SSSTNEFLETECLKVPATEFIGSLKTTIDAIRKATSVVSQFGGFFHDFRLSNAISDCLDL 83
Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
LDSSAD+LSW++SA+QNP AK++STGDLSSDL+TWLSAA+VN +TC+DGFEGTNS++K +
Sbjct: 84 LDSSADELSWTMSASQNPNAKDNSTGDLSSDLRTWLSAAMVNQQTCIDGFEGTNSMVKTV 143
Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQPSE--------------------FPSWLKSEDQNL 273
VSG +NQ+TS + +LL MV PN S FP W K ED+ L
Sbjct: 144 VSGSLNQITSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGGGHPGQSRFPVWFKREDRRL 203
Query: 274 LQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNL 333
QIN + A+ VAADG+G+FT +MDAV APD S+ RYVIYIKKG+Y ENVEIKKKKWNL
Sbjct: 204 SQINGVTANVVVAADGSGNFTRIMDAVETAPDKSLNRYVIYIKKGLYKENVEIKKKKWNL 263
Query: 334 MMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRS 393
MMIGDG+D T+ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQAVALRS
Sbjct: 264 MMIGDGMDVTVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 323
Query: 394 DSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLP 453
DSDLSVFFRC I+GYQD+LYTHTMRQFYREC+I+GTVDF+FGD V+FQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAIKGYQDSLYTHTMRQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLP 383
Query: 454 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSY 513
+QKNTITAQGRKDPNQPTGFSIQFCNISAD+DL PSVN+T TYLGRPWK YSRTIIMQSY
Sbjct: 384 SQKNTITAQGRKDPNQPTGFSIQFCNISADTDLLPSVNSTPTYLGRPWKLYSRTIIMQSY 443
Query: 514 ISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVA 573
ISDAIRP+GWLEWN +FAL+TL+YAE+MN GPGA L++RV WPGYH LN +++A NFTVA
Sbjct: 444 ISDAIRPQGWLEWNQDFALDTLYYAEYMNNGPGASLSERVKWPGYHVLNNSAQAVNFTVA 503
Query: 574 QFIEGNLWLPSTGVKYTAGLG 575
QFIEG+LWLPSTGVKYT+G G
Sbjct: 504 QFIEGDLWLPSTGVKYTSGFG 524
BLAST of Cucsa.323330 vs. TrEMBL
Match:
A0A0B0MDS0_GOSAR (Pectinesterase OS=Gossypium arboreum GN=F383_36646 PE=4 SV=1)
HSP 1 Score: 737.3 bits (1902), Expect = 1.5e-209
Identity = 356/501 (71.06%), Postives = 418/501 (83.43%), Query Frame = 1
Query: 94 ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
+SS + ++TECL VP+++F SL +TID +R+ ++ S F DFRLS AI DCLDL
Sbjct: 24 SSSTNEFLETECLKVPATEFIGSLKTTIDAIRKATSVVSQFGGFFHDFRLSNAIFDCLDL 83
Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
LDSSAD+LSW++SA+QNP AK++STGDLSSDL+TWLSAA+VN +TC+DGFEGTNS++K +
Sbjct: 84 LDSSADELSWTMSASQNPNAKDNSTGDLSSDLRTWLSAAMVNQQTCIDGFEGTNSMVKTV 143
Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQPSE--------------------FPSWLKSEDQNL 273
VSG +NQ+TS + +LL MV PN S FP W K ED+ L
Sbjct: 144 VSGSLNQITSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGGGHPGQNRFPVWFKREDRRL 203
Query: 274 LQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNL 333
LQIN + A+ VAADG+G+FT +MDAV APD S+ RYVIYIKKG+Y ENVEIKKKKWNL
Sbjct: 204 LQINGVTANVVVAADGSGNFTRIMDAVETAPDKSMNRYVIYIKKGLYKENVEIKKKKWNL 263
Query: 334 MMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRS 393
MMIGDG+D T+ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQAVALRS
Sbjct: 264 MMIGDGMDVTVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 323
Query: 394 DSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLP 453
DSDLSVFFRC I+GYQD+LYTHTMRQFYREC+I+GTVDF+FGD V+FQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAIKGYQDSLYTHTMRQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLP 383
Query: 454 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSY 513
+QKNTITAQGRKDPNQPTGFSIQFCNISAD+DL PSVN+T TYLGRPWK YSRTIIMQSY
Sbjct: 384 SQKNTITAQGRKDPNQPTGFSIQFCNISADTDLLPSVNSTPTYLGRPWKLYSRTIIMQSY 443
Query: 514 ISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVA 573
ISDAIRP+GWLEWN +FAL+TL+YAE+MN GPGA L++RV WPGYH LN +++A NFTVA
Sbjct: 444 ISDAIRPQGWLEWNQDFALDTLYYAEYMNNGPGASLSERVKWPGYHVLNNSAQAVNFTVA 503
Query: 574 QFIEGNLWLPSTGVKYTAGLG 575
QFIEG+LWLPSTGVKYT+G G
Sbjct: 504 QFIEGDLWLPSTGVKYTSGFG 524
BLAST of Cucsa.323330 vs. TrEMBL
Match:
A0A0D2QI13_GOSRA (Pectinesterase OS=Gossypium raimondii GN=B456_001G018300 PE=4 SV=1)
HSP 1 Score: 737.3 bits (1902), Expect = 1.5e-209
Identity = 355/501 (70.86%), Postives = 418/501 (83.43%), Query Frame = 1
Query: 94 ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
+S+ + ++TECL VP+++F SL +TID +R+ ++ S F DFRLS AISDCLDL
Sbjct: 24 SSTTNEFLETECLKVPATEFIGSLKTTIDAIRKATSVVSQFGGFFHDFRLSNAISDCLDL 83
Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
LDSSAD+LSW++ A+QNP AK++STGDLSSDL+TWLSAA+VN +TC+DGFEGTNS++K +
Sbjct: 84 LDSSADELSWTMFASQNPNAKDNSTGDLSSDLRTWLSAAMVNQQTCIDGFEGTNSMVKTV 143
Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQPSE--------------------FPSWLKSEDQNL 273
VSG +NQ+TS + +LL MV PN S FP W K ED+ L
Sbjct: 144 VSGSLNQITSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGGGHPGQNRFPVWFKREDRRL 203
Query: 274 LQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNL 333
LQIN + A+ VAADG+G+FT +MDAV APD S+ RYVIYIK+G+Y ENVEIKKKKWNL
Sbjct: 204 LQINGVTANVVVAADGSGNFTRIMDAVETAPDKSLNRYVIYIKRGLYKENVEIKKKKWNL 263
Query: 334 MMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRS 393
MMIGDG+D T+ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQAVALRS
Sbjct: 264 MMIGDGMDVTVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 323
Query: 394 DSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLP 453
DSDLSVFFRC I+GYQD+LYTHTMRQFYREC+I+GTVDF+FGD V+FQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAIKGYQDSLYTHTMRQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLP 383
Query: 454 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSY 513
NQKNTITAQGRKDPNQPTGFSIQFCNISAD+DL PSVN+T TYLGRPWK YSRTIIMQSY
Sbjct: 384 NQKNTITAQGRKDPNQPTGFSIQFCNISADTDLLPSVNSTPTYLGRPWKLYSRTIIMQSY 443
Query: 514 ISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVA 573
ISDAIRP+GWLEWN +FAL+TL+YAE+MN GPGA L++RV WPGYH LN +++A NFTVA
Sbjct: 444 ISDAIRPQGWLEWNQDFALDTLYYAEYMNNGPGASLSERVKWPGYHVLNNSAQAVNFTVA 503
Query: 574 QFIEGNLWLPSTGVKYTAGLG 575
QFIEG+LWLPSTGVKYT+G G
Sbjct: 504 QFIEGDLWLPSTGVKYTSGFG 524
BLAST of Cucsa.323330 vs. TrEMBL
Match:
R9QPU2_GOSBA (Pectinesterase OS=Gossypium barbadense PE=2 SV=1)
HSP 1 Score: 736.9 bits (1901), Expect = 1.9e-209
Identity = 355/501 (70.86%), Postives = 419/501 (83.63%), Query Frame = 1
Query: 94 ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
+SS + ++TECL VP+++F SL +TID +R+ ++ S F DFRLS AISDCLDL
Sbjct: 24 SSSTNEFLETECLKVPATEFIGSLKTTIDAIRKATSVVSQFGGFFHDFRLSNAISDCLDL 83
Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
LDSSAD+LSW++SA+QNP AK++STGDLSSDL+TWLSAA+VN +TC+DGFEGTNS++K +
Sbjct: 84 LDSSADELSWTMSASQNPNAKDNSTGDLSSDLRTWLSAAMVNQQTCIDGFEGTNSMVKTV 143
Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQPSE--------------------FPSWLKSEDQNL 273
VSG +NQ+TS + +LL MV PN S FP W K ED+ L
Sbjct: 144 VSGSLNQITSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGGGHPGQSRFPVWFKREDRRL 203
Query: 274 LQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNL 333
LQIN + A+ VAADG+G+FT +MDAV APD S+ RYVIYIKKG+Y ENVEIKKKKWNL
Sbjct: 204 LQINGVTANVVVAADGSGNFTRIMDAVETAPDKSMNRYVIYIKKGLYKENVEIKKKKWNL 263
Query: 334 MMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRS 393
+MIGDG+D T+ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQAVALRS
Sbjct: 264 VMIGDGMDVTVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRS 323
Query: 394 DSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLP 453
DSDLSVFFRC I+GYQD+LYTHTMRQFYREC+I+GTVDF+FGD V+FQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAIKGYQDSLYTHTMRQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLP 383
Query: 454 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSY 513
+QKNTITAQGRKDPNQPTGFSIQFCNISAD+DL PSVN+T TYLGRPW+ YSRTIIMQSY
Sbjct: 384 SQKNTITAQGRKDPNQPTGFSIQFCNISADTDLLPSVNSTPTYLGRPWRLYSRTIIMQSY 443
Query: 514 ISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVA 573
ISDAIRP+GWLEWN +FAL+TL+YAE+MN GPGA L++RV WPGYH LN +++A NFTVA
Sbjct: 444 ISDAIRPQGWLEWNQDFALDTLYYAEYMNNGPGASLSERVKWPGYHVLNNSAQAVNFTVA 503
Query: 574 QFIEGNLWLPSTGVKYTAGLG 575
QFIEG+LWLPSTGVKYT+G G
Sbjct: 504 QFIEGDLWLPSTGVKYTSGFG 524
BLAST of Cucsa.323330 vs. TAIR10
Match:
AT3G43270.1 (AT3G43270.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 679.5 bits (1752), Expect = 1.8e-195
Identity = 323/498 (64.86%), Postives = 407/498 (81.73%), Query Frame = 1
Query: 94 ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
A S DLVQ ECL VP +F+ + + +D + + +AI S F K G R+S AI DC+DL
Sbjct: 28 AFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDL 87
Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
LDS+A++LSW +SA+Q+P K++STGD+ SDL+TW+SAA+ N +TC+DGFEGTN IIK +
Sbjct: 88 LDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKI 147
Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQP-----------------SEFPSWLKSEDQNLLQI 273
V+GG++++ + + +LL+MV S P++P S+FPSW+K D+ LLQ
Sbjct: 148 VAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQT 207
Query: 274 NDLA-ADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMM 333
+++ ADA VAADGTG+FT + DAVLAAPD S +RYVI++K+GVY+ENVEIKKKKWN+MM
Sbjct: 208 DNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMM 267
Query: 334 IGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDS 393
+GDGIDAT+I+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAG EKHQAVA+RSD+
Sbjct: 268 VGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDT 327
Query: 394 DLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQ 453
DL VF+RC +RGYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQ
Sbjct: 328 DLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQ 387
Query: 454 KNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYIS 513
KN+ITAQGRKDPN+PTGF+IQF NI+AD+DL ++NTTATYLGRPWK YSRT+ MQ+Y+S
Sbjct: 388 KNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMS 447
Query: 514 DAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQF 573
DAI P GWLEWN NFAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTV+Q
Sbjct: 448 DAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQL 507
BLAST of Cucsa.323330 vs. TAIR10
Match:
AT4G33220.1 (AT4G33220.1 pectin methylesterase 44)
HSP 1 Score: 670.2 bits (1728), Expect = 1.1e-192
Identity = 325/521 (62.38%), Postives = 402/521 (77.16%), Query Frame = 1
Query: 81 CCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGD 140
C L+ ML S + Q + L VP+S+F +S+ + + V+RQV +I S F+ GD
Sbjct: 3 CLKYFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 62
Query: 141 FRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCM 200
RL A+SDCLDLLD S+++L+WS SA++NPK K + TGD+ SD +TWLSAA+ N TCM
Sbjct: 63 RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 122
Query: 201 DGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMV-------------------KSIP----- 260
+GF+GT+ ++K LV+G ++QL S L +LL +V K+ P
Sbjct: 123 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 182
Query: 261 ----NQPSEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYV 320
++ +FP W++ +D+ LL+ N D +VA DGTG+FT +MDA+ APD S R+V
Sbjct: 183 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 242
Query: 321 IYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIA 380
IYIKKG+YLENVEIKKKKWN++M+GDGID T+ISGNRSFIDGWTTFRSATFAVSGRGF+A
Sbjct: 243 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLA 302
Query: 381 RDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDF 440
RDITF+NTAG EKHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF
Sbjct: 303 RDITFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDF 362
Query: 441 LFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNT 500
+FGD TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +NT
Sbjct: 363 IFGDGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNT 422
Query: 501 TATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKR 560
T TYLGRPWK YSRT+ +++ +SD +RPEGWLEWNA+FAL+TLFY EFMNYGPG+GL+ R
Sbjct: 423 TRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSR 482
Query: 561 VNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
V WPGYH N + +A NFTV+QFI+GNLWLPSTGV ++ GL
Sbjct: 483 VKWPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523
BLAST of Cucsa.323330 vs. TAIR10
Match:
AT3G05620.1 (AT3G05620.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 436.4 bits (1121), Expect = 2.7e-122
Identity = 221/452 (48.89%), Postives = 296/452 (65.49%), Query Frame = 1
Query: 142 RLSTAISDCLDLLDSSADQLSWSLSATQNPKAK--------NHSTGDLSSDLKTWLSAAV 201
R AI DC +L+ S +L+WS+ +H +LKTWLSAA+
Sbjct: 100 REQVAIEDCKELVGFSVTELAWSMLEMNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAAM 159
Query: 202 VNPETCMDGFEGTNSIIKGLVSGG---VNQLTSQLYDLLSMVKSIPNQPSE------FPS 261
N +TC++GFEGT + L+ G V QL S + D+ + + ++P + S P
Sbjct: 160 SNQDTCLEGFEGTERKYEELIKGSLRQVTQLVSNVLDMYTQLNALPFKASRNESVIASPE 219
Query: 262 WLKSEDQNLLQIND---LAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYL 321
WL D++L+ +D + + VA DG G + + +A+ AP++S +RYVIY+KKGVY
Sbjct: 220 WLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYK 279
Query: 322 ENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTA 381
EN+++KKKK N+M++GDGI TII+G+R+F+ G TTFR+AT AVSGRGFIA+DITF NTA
Sbjct: 280 ENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTA 339
Query: 382 GAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVF 441
G + QAVALR DSD S F+RC + GYQDTLY H++RQFYR+C+I GT+DF+FG+ V
Sbjct: 340 GPQNRQAVALRVDSDQSAFYRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVL 399
Query: 442 QNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPW 501
QNC I + LP QK TITAQGRK PNQ TGF IQ + A T TYLGRPW
Sbjct: 400 QNCKIYTRVPLPLQKVTITAQGRKSPNQNTGFVIQNSYVLA---------TQPTYLGRPW 459
Query: 502 KEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRL 561
K YSRT+ M +Y+S ++P GWLEW NFAL+TL+Y E+ N GPG + RV WPGYH +
Sbjct: 460 KLYSRTVYMNTYMSQLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIM 519
Query: 562 NQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
++ + A +FTV FI+G WLP+TGV +TAGL
Sbjct: 520 DKRT-ALSFTVGSFIDGRRWLPATGVTFTAGL 541
BLAST of Cucsa.323330 vs. TAIR10
Match:
AT3G49220.1 (AT3G49220.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 433.0 bits (1112), Expect = 3.0e-121
Identity = 236/529 (44.61%), Postives = 323/529 (61.06%), Query Frame = 1
Query: 75 TQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPF 134
T+ + C ++ FP L ASS DL+ +++ FS++L S+ + M
Sbjct: 94 TRFPELCVDSLMDFPGSLAASSSKDLIHVT-VNMTLHHFSHALYSSASLSFVDMPP---- 153
Query: 135 SKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVV 194
R +A C++LLD S D LS +LS+ + AK D+ TWLSAA+
Sbjct: 154 -------RARSAYDSCVELLDDSVDALSRALSSVVSSSAK-------PQDVTTWLSAALT 213
Query: 195 NPETCMDGFEGTNSIIKGLVSGGV-NQLTSQLYDLLSMVKS------------------- 254
N +TC +GF+G + GGV + +T+ L +L +V +
Sbjct: 214 NHDTCTEGFDGVD-------DGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPI 273
Query: 255 -------IPNQPSEFPSWLKSEDQNLLQ--INDLAADATVAADGTGDFTNVMDAVLAAPD 314
+ + +FP W++ +++ +L+ ++ + AD V+ DG G + +A+ AP
Sbjct: 274 QNRRLLGVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQ 333
Query: 315 NSIRRYVIYIKKGVYLE-NVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFA 374
NS RR +IY+K G Y E N+++ +KK NLM +GDG T+ISG +S D TTF +A+FA
Sbjct: 334 NSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFA 393
Query: 375 VSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYREC 434
+G GFIARDITFEN AG KHQAVALR +D +V +RC I GYQDTLY H+ RQF+REC
Sbjct: 394 ATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFREC 453
Query: 435 QISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADS 494
I GTVDF+FG+A VV QNCSI A+K + QKNTITAQ RKDPNQ TG SI + A S
Sbjct: 454 DIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAAS 513
Query: 495 DLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYG 554
DL+ + +T TYLGRPWK +SRT+ M SYI + GWLEWN FAL+TL+Y E++N G
Sbjct: 514 DLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSG 573
Query: 555 PGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
PG+GL +RV+WPGY +N T+EA FTVA+FI G+ WLPSTGV + AGL
Sbjct: 574 PGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596
BLAST of Cucsa.323330 vs. TAIR10
Match:
AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)
HSP 1 Score: 426.0 bits (1094), Expect = 3.7e-119
Identity = 226/456 (49.56%), Postives = 296/456 (64.91%), Query Frame = 1
Query: 142 RLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLS-SDLKTWLSAAVVNPETCM 201
R+ +A CL+LLD S D L+ +LS+ +GD S SD+ TWLS+A+ N +TC
Sbjct: 136 RVRSAYDSCLELLDDSVDALTRALSSVVVV------SGDESHSDVMTWLSSAMTNHDTCT 195
Query: 202 DGF---EGTNSIIKGLVSGGVNQLTSQLYDLLSM----VKSIPNQP-------------S 261
DGF EG +K V G V L+ + + L++ VK + P
Sbjct: 196 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETE 255
Query: 262 EFPSWLKSEDQNLL--QINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKG 321
E P+WLK ED+ LL + + AD TV+ DG+G F + +A+ AP++S RR+VIY+K G
Sbjct: 256 ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAG 315
Query: 322 VYLE-NVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITF 381
Y E N+++ +KK NLM IGDG T+I+G +S D TTF +ATFA +G GFI RD+TF
Sbjct: 316 RYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTF 375
Query: 382 ENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDA 441
EN AG KHQAVALR D +V +RC I GYQD LY H+ RQF+REC+I GTVDF+FG+A
Sbjct: 376 ENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNA 435
Query: 442 TVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYL 501
V+ Q+C+I A+K + QK TITAQ RKDPNQ TG SI C + A DL+ S + TYL
Sbjct: 436 AVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYL 495
Query: 502 GRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPG 561
GRPWK YSR + M S + D I P GWLEWN FAL++L+Y E+MN G G+G+ +RV WPG
Sbjct: 496 GRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPG 555
Query: 562 YHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
YH + T EA+ FTVAQFI G+ WLPSTGV + +GL
Sbjct: 556 YHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585
BLAST of Cucsa.323330 vs. NCBI nr
Match:
gi|778674515|ref|XP_004146188.2| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus])
HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 1
Query: 1 MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60
MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA
Sbjct: 1 MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60
Query: 61 KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120
KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST
Sbjct: 61 KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120
Query: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180
IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD
Sbjct: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180
Query: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240
LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS
Sbjct: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240
Query: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300
EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY
Sbjct: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300
Query: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360
LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT
Sbjct: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360
Query: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420
AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV
Sbjct: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420
Query: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480
FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP
Sbjct: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480
Query: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540
WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR
Sbjct: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540
Query: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN
Sbjct: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576
BLAST of Cucsa.323330 vs. NCBI nr
Match:
gi|659095151|ref|XP_008448427.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo])
HSP 1 Score: 1054.3 bits (2725), Expect = 7.8e-305
Identity = 531/578 (91.87%), Postives = 539/578 (93.25%), Query Frame = 1
Query: 1 MDFHTNPSPQNVNVAFKSTRYLPNPLPLK--FLSLTFQLDSSFTSPIFVLILLHHPIHTG 60
MDF P P + + P P K F L L FT FVLILLHHPIHTG
Sbjct: 1 MDFSDKPFPTKRECSIQINALSPQPPAFKVPFPHLPTGLFIYFTD--FVLILLHHPIHTG 60
Query: 61 QAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLL 120
Q KGSSIIPILMAKLTTQ LQFCC+ ILLFPMLLPASSP DLVQTECLSVPSSQFSNSLL
Sbjct: 61 QGKGSSIIPILMAKLTTQILQFCCIFILLFPMLLPASSPADLVQTECLSVPSSQFSNSLL 120
Query: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHST 180
STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAK+HST
Sbjct: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKDHST 180
Query: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQ 240
GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGG+NQLTSQLYDLLSMVKSIPNQ
Sbjct: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGINQLTSQLYDLLSMVKSIPNQ 240
Query: 241 PSEFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKG 300
PSEFPSWLKSED+NLLQ+NDLAADATVAADGTGDFTNVMDAVLAAPD S RRYVIYIKKG
Sbjct: 241 PSEFPSWLKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDKSTRRYVIYIKKG 300
Query: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360
VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE
Sbjct: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360
Query: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420
NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT
Sbjct: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420
Query: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480
VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG
Sbjct: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480
Query: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540
RPWKEYSRTIIMQSYISDAIRPEGWLEWN NFALNTLFYAEFMNYGPGAGLAKRVNWPGY
Sbjct: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540
Query: 541 HRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
HRLNQTSEATNFTVAQFIEGN+WLPSTGVKYTAGLGAN
Sbjct: 541 HRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN 576
BLAST of Cucsa.323330 vs. NCBI nr
Match:
gi|694388007|ref|XP_009369733.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B [Pyrus x bretschneideri])
HSP 1 Score: 743.8 bits (1919), Expect = 2.2e-211
Identity = 366/505 (72.48%), Postives = 423/505 (83.76%), Query Frame = 1
Query: 78 LQFCCLLILLFPMLLPASSPP--DLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFS 137
L F LL + +P +S D +Q+ECL VP+S+F+ SL TID V+QV I S F+
Sbjct: 7 LFFLVLLCVCCKSRIPFASCSFTDDIQSECLKVPASEFAGSLRDTIDAVQQVATILSQFA 66
Query: 138 KLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVN 197
GDFRLS AISDCLDLLD SAD+L+WSLSA+QN K K++STG LSSDL+TWLSAA+VN
Sbjct: 67 NAFGDFRLSNAISDCLDLLDFSADELNWSLSASQNQKGKSNSTGKLSSDLRTWLSAALVN 126
Query: 198 PETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQP-----SEFPSWLKSED 257
+TC DGFEGTNSI+KGLVS G+NQ+TS + DLL+ V PN + P+W+K+ED
Sbjct: 127 QDTCSDGFEGTNSIVKGLVSTGLNQVTSLVQDLLTQVH--PNSDHQGPNGQIPTWVKTED 186
Query: 258 QNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKK 317
+ LLQ + + DA VA DGTG+FTNVMDAVLAAPD S+ RYVIYIKKG Y ENVEIKKKK
Sbjct: 187 RKLLQADGVNVDAVVAQDGTGNFTNVMDAVLAAPDYSMSRYVIYIKKGTYKENVEIKKKK 246
Query: 318 WNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVA 377
WNLMMIGDG+DATIISGNRS+IDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQAVA
Sbjct: 247 WNLMMIGDGMDATIISGNRSYIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVA 306
Query: 378 LRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKK 437
LRSDSDLSVF+RC IRGYQDTLY HTMRQFYR+C+ISGTVDF+FGDATVVFQNC ILAKK
Sbjct: 307 LRSDSDLSVFYRCEIRGYQDTLYAHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKK 366
Query: 438 GLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIM 497
GLPNQKN++TAQGRKDPN+PTG SIQFCNI+AD+DL P VN+T TYLGRPWK YSRT+IM
Sbjct: 367 GLPNQKNSVTAQGRKDPNEPTGISIQFCNITADADLLPFVNSTFTYLGRPWKLYSRTVIM 426
Query: 498 QSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNF 557
QS++S+AIRPEGWLEWNA+FAL+TL Y E+MNYGPGAGL RV WPGY N++++ N+
Sbjct: 427 QSFLSNAIRPEGWLEWNADFALSTLSYGEYMNYGPGAGLGSRVKWPGYQVFNESTQVKNY 486
Query: 558 TVAQFIEGNLWLPSTGVKYTAGLGA 576
TVAQFIEGNLWLP TGVKYTAG GA
Sbjct: 487 TVAQFIEGNLWLPPTGVKYTAGFGA 509
BLAST of Cucsa.323330 vs. NCBI nr
Match:
gi|645274002|ref|XP_008242144.1| (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B [Prunus mume])
HSP 1 Score: 740.0 bits (1909), Expect = 3.2e-210
Identity = 358/480 (74.58%), Postives = 416/480 (86.67%), Query Frame = 1
Query: 99 DLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSA 158
D +QT CL VP+S+F+ SL TID V+QV +I SPF+ GDFRLS AISDCLDLLD SA
Sbjct: 37 DDLQTGCLKVPASEFAGSLKDTIDAVQQVASILSPFANAFGDFRLSNAISDCLDLLDFSA 96
Query: 159 DQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGV 218
D+L+WS+SA+QN K KN+STG LSSDL+TWLSAA+VN +TC +GFEGTNSI++GLVS G+
Sbjct: 97 DELNWSISASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLVSAGL 156
Query: 219 NQLTSQLYDLLSMVKSIPNQPS---EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTN 278
+Q+TS + +LL+ V NQ + PSW+K+ED+ LLQ + ++ DA VA DGTG+FTN
Sbjct: 157 DQVTSLVQELLTQVHPNSNQQGPNGQIPSWVKTEDRKLLQADGVSVDAIVAQDGTGNFTN 216
Query: 279 VMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGW 338
V DAVLAAPD S+RRYVIYIK+G Y ENVEIKKKKWNLMMIGDG+DATIISGNR+F+DGW
Sbjct: 217 VTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRNFVDGW 276
Query: 339 TTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTH 398
TTFRSATFAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVF+RC IRGYQDTLYTH
Sbjct: 277 TTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTH 336
Query: 399 TMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSI 458
TMRQFYR+C+ISGTVDF+FGDATVVFQNC ILAKKGLPNQKN+ITAQGRKD N+PTG SI
Sbjct: 337 TMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDLNEPTGISI 396
Query: 459 QFCNISADSDLK-PSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNT 518
QFCNI+ADSDL+ SVN+T TYLGRPWK YSRT+IMQS++S+ IRPEGWLEWN +FALN+
Sbjct: 397 QFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNS 456
Query: 519 LFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLG 575
LFY E+MNYGPGAGL RV WPGY N++++A N+TVAQFIEGNLWLPSTGVKYTA G
Sbjct: 457 LFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTADFG 516
BLAST of Cucsa.323330 vs. NCBI nr
Match:
gi|6093744|sp|Q43062.1|PME_PRUPE (RecName: Full=Pectinesterase/pectinesterase inhibitor PPE8B; Includes: RecName: Full=Pectinesterase inhibitor PPE8B; AltName: Full=Pectin methylesterase inhibitor PPE8B; Includes: RecName: Full=Pectinesterase PPE8B; Short=PE PPE8B; AltName: Full=Pectin methylesterase PPE8B; Flags: Precursor)
HSP 1 Score: 738.4 bits (1905), Expect = 9.4e-210
Identity = 364/533 (68.29%), Postives = 436/533 (81.80%), Query Frame = 1
Query: 46 FVLILLHHPIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTEC 105
++L+ H+P+ G K ++ +L A ++ + F + S D +QT+C
Sbjct: 3 YLLMASHNPLPAG--KQLLLLVLLCAFFSSSFIPFA------------SCSITDDLQTQC 62
Query: 106 LSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSL 165
L V +++F+ SL TID V+QV +I S F+ GDFRL+ AISDCLDLLD SAD+L+WSL
Sbjct: 63 LKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADELNWSL 122
Query: 166 SATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQL 225
SA+QN K KN+STG LSSDL+TWLSAA+VN +TC +GFEGTNSI++GL+S G+ Q+TS +
Sbjct: 123 SASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLV 182
Query: 226 YDLLSMVKSIPNQPS---EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLA 285
+LL+ V NQ + PSW+K++D+ LLQ + ++ DA VA DGTG+FTNV DAVLA
Sbjct: 183 QELLTQVHPNSNQQGPNGQIPSWVKTKDRKLLQADGVSVDAIVAQDGTGNFTNVTDAVLA 242
Query: 286 APDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSAT 345
APD S+RRYVIYIK+G Y ENVEIKKKKWNLMMIGDG+DATIISGNRSF+DGWTTFRSAT
Sbjct: 243 APDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSAT 302
Query: 346 FAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYR 405
FAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVF+RC IRGYQDTLYTHTMRQFYR
Sbjct: 303 FAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYR 362
Query: 406 ECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISA 465
+C+ISGTVDF+FGDATVVFQNC ILAKKGLPNQKN+ITAQGRKDPN+PTG SIQFCNI+A
Sbjct: 363 DCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITA 422
Query: 466 DSDLK-PSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFM 525
DSDL+ SVN+T TYLGRPWK YSRT+IMQS++S+ IRPEGWLEWN +FALN+LFY E+M
Sbjct: 423 DSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYM 482
Query: 526 NYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLG 575
NYGPGAGL RV WPGY N++++A N+TVAQFIEGNLWLPSTGVKYTA G
Sbjct: 483 NYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFG 521
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| PME_PRUPE | 5.9e-212 | 68.29 | Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1 | [more] |
| PME32_ARATH | 3.3e-194 | 64.86 | Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=P... | [more] |
| PME44_ARATH | 2.0e-191 | 62.38 | Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana GN=P... | [more] |
| PME22_SOLLC | 6.5e-126 | 47.44 | Pectinesterase 2.2 OS=Solanum lycopersicum GN=PME2.2 PE=3 SV=1 | [more] |
| PME1_SOLLC | 1.6e-124 | 44.34 | Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9 PE=1 SV=5 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G43270.1 | 1.8e-195 | 64.86 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT4G33220.1 | 1.1e-192 | 62.38 | pectin methylesterase 44 | [more] |
| AT3G05620.1 | 2.7e-122 | 48.89 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT3G49220.1 | 3.0e-121 | 44.61 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT5G53370.1 | 3.7e-119 | 49.56 | pectin methylesterase PCR fragment F | [more] |
