Lsi06G006940 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G006940
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	4-hydroxy-4-methyl-2-oxoglutarate aldolase
Location	chr06 : 11029609 .. 11031481 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGAGGAATGCAAAATGCTGTGGAAAGCTTTTCATTCTCAAGAAGTATTAAGGCTTTTGGATGCAGCCATCAGGTGGTACTCACACTAACAATAAAGTTCAAAGGAAAAAACCCCTCATTTTCGGTTGGCTGAATCGGCTTTCCTTCAGTCATCTCTTCCTCCCCTGTTCTCGAGTTCAAACTTTATGGCTTAGGCTGCTACGTTTATTCAACTTACCCTTGCTGATGCCTAGAAGAGTGAAGGATTTTATGTTGTTGACTTTATGTTATGTGGTCACAATCCTAGGGATAGAGCTAAGAGCTTGTGGATCAACGGCTTTAGTGCTATTTGATGGGGTTTAAGGCTGAAAGACATAAAAACTTGGATCTACTACAGGAGGTTGTAGTTTTGTTCCTTCTTCTTAGTGCCTTGTGTCCCAGTTGTAGAACCTTGTACTGAGTAAGTCTGATGCAACTTGATAATTCATTTTTTATGAGAATCAGTAACTTTTGAAATGCCAAGTATGGTAGTTTGAAAGCAACGTTTATATTTTCTAGTTTTTGAAAAAGTATAAACATTTTAAGATCTTAATTGCTTATTTGGAACCCCAAGGAAATAAATCAACTCCACTTTATAAGAATTGAACAGCAAATAAAAGAATGAGATCGAATGAACCCTTGATCTTGTCATTGACTTGTGTGATCAAGAGCCTTCAAAGAATTGAAGTTTTACTCTGAAGTGTCTGAGAAATAAACTACAATCATTATTCATTACTTAATACTGAGAATGAGATTAATGAGCTGGAGTGAAATGATAAACAGAGATGTACTTCTGTTTACTACTGAGTGTATTTACTGTTAAATCATTGCATATGGTGATATGGATTTTGAATGCCCTTCAAGATTCACCAGTGTATGATTGAAAATCCATTAAAAACACATAAAAGCTCTTAATTTTTCTACATGCTTGTGATTGTGTTTTATCTGCAAAATCTTTTATGTTTTCTTTTTTTAGTTCAACAAATGACCAAATGGGACAATTTCTGCTTTAACTGAATTCAATATTTGTCTACAAACTTAAGGTGCTTGGAGGAAATGGCCTTGGTTACCACTGCAGAAGTGTGTGATGCTAATCCACAGCTCATTGTGAGTGGTGAACTTCGGGCCCTCGAACCAATTTTCCAGATATATGGCCGACGCCAAGTCTTTTCAGGACCAGTAGTCACACTGAAGGTGTTTGAAGACAATGTCTTGATTCGTGAGTTCCTCGAGGAGAAGGGTAATGGCAGAGTTCTTGTTGTGGACGGGGGCGGTAGTAAGAGGTGTGCAATATTGGGTGGCAATCCTGTTGTCCAAGCTCAGAACAATGGTTGGGCTGGTATAGTTGTGAATGGATGTGTTAGAGATGTGGATGAAATAAATGGTTGTGATATCGGGGTAAGGGCTCTCGCGTCACATCCCATGAAAGCCTACAAAAAGCGGGTCGGAGAAAAGCACGTTCCGATAACTGTAGCTGGGACAAGGATCATGGATGGGGAATGGCTGTATGCAGATACTGATGGGATTCTGGTTTCACGAACCGAGCTGTCCGTTTAGAGAACTGTTGATTAATTCCCTTGCAAAGATTTTGATGGCAGGATGATATTGTTATACGAAGGCGAGAGCGAGGACGAGGGCAAGAAGTTGAGAGTAATGCTGTTGTTGTCTTCTTGTGGACCCTAAAATTCAACTCCTTGTTATATTTATCACTAAAATTATGTTATGGTTGAGCTTAATATGATGTTGTGTTCTGTAAAAGATGAACTGCTGCATTAATCTCTTAATAGTGTTAAGAGGCCTTTACTGTTGTAAGCAACATATTGTCTTATCAATGAAAAATACTTGATGGAA

mRNA sequence

ATGAAAGAGGAATGCAAAATGCTGTGGAAAGCTTTTCATTCTCAAGAAGTATTAAGGCTTTTGGATGCAGCCATCAGGTGGTGCTTGGAGGAAATGGCCTTGGTTACCACTGCAGAAGTGTGTGATGCTAATCCACAGCTCATTGTGAGTGGTGAACTTCGGGCCCTCGAACCAATTTTCCAGATATATGGCCGACGCCAAGTCTTTTCAGGACCAGTAGTCACACTGAAGGTGTTTGAAGACAATGTCTTGATTCGTGAGTTCCTCGAGGAGAAGGGTAATGGCAGAGTTCTTGTTGTGGACGGGGGCGGTAGTAAGAGGTGTGCAATATTGGGTGGCAATCCTGTTGTCCAAGCTCAGAACAATGGTTGGGCTGGTATAGTTGTGAATGGATGTGTTAGAGATGTGGATGAAATAAATGGTTGTGATATCGGGGTAAGGGCTCTCGCGTCACATCCCATGAAAGCCTACAAAAAGCGGGTCGGAGAAAAGCACGTTCCGATAACTGTAGCTGGGACAAGGATCATGGATGGGGAATGGCTGTATGCAGATACTGATGGGATTCTGGTTTCACGAACCGAGCTGTCCGTTTAGAGAACTGTTGATTAATTCCCTTGCAAAGATTTTGATGGCAGGATGATATTGTTATACGAAGGCGAGAGCGAGGACGAGGGCAAGAAGTTGAGAGTAATGCTGTTGTTGTCTTCTTGTGGACCCTAAAATTCAACTCCTTGTTATATTTATCACTAAAATTATGTTATGGTTGAGCTTAATATGATGTTGTGTTCTGTAAAAGATGAACTGCTGCATTAATCTCTTAATAGTGTTAAGAGGCCTTTACTGTTGTAAGCAACATATTGTCTTATCAATGAAAAATACTTGATGGAA

Coding sequence (CDS)

ATGAAAGAGGAATGCAAAATGCTGTGGAAAGCTTTTCATTCTCAAGAAGTATTAAGGCTTTTGGATGCAGCCATCAGGTGGTGCTTGGAGGAAATGGCCTTGGTTACCACTGCAGAAGTGTGTGATGCTAATCCACAGCTCATTGTGAGTGGTGAACTTCGGGCCCTCGAACCAATTTTCCAGATATATGGCCGACGCCAAGTCTTTTCAGGACCAGTAGTCACACTGAAGGTGTTTGAAGACAATGTCTTGATTCGTGAGTTCCTCGAGGAGAAGGGTAATGGCAGAGTTCTTGTTGTGGACGGGGGCGGTAGTAAGAGGTGTGCAATATTGGGTGGCAATCCTGTTGTCCAAGCTCAGAACAATGGTTGGGCTGGTATAGTTGTGAATGGATGTGTTAGAGATGTGGATGAAATAAATGGTTGTGATATCGGGGTAAGGGCTCTCGCGTCACATCCCATGAAAGCCTACAAAAAGCGGGTCGGAGAAAAGCACGTTCCGATAACTGTAGCTGGGACAAGGATCATGGATGGGGAATGGCTGTATGCAGATACTGATGGGATTCTGGTTTCACGAACCGAGCTGTCCGTTTAG

Protein sequence

MKEECKMLWKAFHSQEVLRLLDAAIRWCLEEMALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEEKGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALASHPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV

Homology

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BLAST of Lsi06G006940 vs. Swiss-Prot
Match: RRAA2_ARATH (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 OS=Arabidopsis thaliana GN=At5g16450 PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 2.6e-79
Identity = 141/166 (84.94%), Postives = 156/166 (93.98%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MA VTTAEVCDAN +LI SG+LRAL+PIFQIYGRRQ+FSGPVVT+KVFEDN LIR+F+EE
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS+RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HP+KA KK +GE+ VP+ +AGTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Lsi06G006940 vs. Swiss-Prot
Match: RRAA1_ARATH (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 1 OS=Arabidopsis thaliana GN=At3g02770 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 1.9e-77
Identity = 138/166 (83.13%), Postives = 153/166 (92.17%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MA VTTAEVCDAN ++I SG+LRAL+P+FQIYGRRQ+FSGPVVT+KVFEDN LIR FLEE
Sbjct: 1   MAFVTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGI+VNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HP+KA KK +GE+ V + +AGTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Lsi06G006940 vs. Swiss-Prot
Match: RRAA3_ARATH (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 OS=Arabidopsis thaliana GN=At5g56260 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 2.4e-64
Identity = 116/166 (69.88%), Postives = 139/166 (83.73%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MA   TAE CD+N +LI +G+LRAL PIF+IYG+R+ FSGP+VTLKVFEDNVL+R  LE 
Sbjct: 1   MAAFATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLET 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KG G VLV+DGGGS RCA++GGN    AQNNGW+GIVVNGCVRDVDEIN CD+GVRAL S
Sbjct: 61  KGEGGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           +P+K+ KK  GEK+VP+ + GT I DGEWLYAD+DGIL+S+TELSV
Sbjct: 121 NPLKSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELSV 166

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BLAST of Lsi06G006940 vs. Swiss-Prot
Match: RRAAH_CUPPJ (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) GN=Reut_A1963 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.2e-49
Identity = 85/159 (53.46%), Postives = 115/159 (72.33%), Query Frame = 1


Query: 37  TAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEEKGNGR 96
           T ++CDAN   +  G LR + P+F+ +G++Q F+GP  TLK+FEDN L+RE LE  G GR
Sbjct: 5   TTDLCDANEPRLADGTLRVMAPVFRAFGKQQAFAGPAATLKIFEDNSLVREALESPGQGR 64

Query: 97  VLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALASHPMKA 156
           VLVVDGGGS RCA++GGN  + A+ NGW GI+VNGCVRD  E++ CDIG+RALA+HP K+
Sbjct: 65  VLVVDGGGSLRCALVGGNLGLLAEKNGWVGIIVNGCVRDTGELDVCDIGIRALAAHPQKS 124

Query: 157 YKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTEL 196
            K+  GE+ V + + G  +  G W+Y D DG+LVS  +L
Sbjct: 125 QKRGAGERDVAVQMPGAVVRPGNWIYVDADGVLVSEEKL 163

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BLAST of Lsi06G006940 vs. Swiss-Prot
Match: RRAAH_BURM1 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Burkholderia multivorans (strain ATCC 17616 / 249) GN=Bmul_1223 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 3.7e-49
Identity = 91/160 (56.88%), Postives = 115/160 (71.88%), Query Frame = 1


Query: 37  TAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEEKGNGR 96
           T ++CDA+   + +G LR L+P  + YG    F+GP  TLKVFEDN L+R  LE+ G GR
Sbjct: 5   TTDLCDAHEDKLAAGTLRVLQPALRPYGGAARFAGPAATLKVFEDNTLVRAALEQDGGGR 64

Query: 97  VLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALASHPMKA 156
           VLVVDGG S RCA++GGN    A+ NGWAGIVV+GCVRD  E+  C +GV ALA+HP K+
Sbjct: 65  VLVVDGGASLRCALVGGNLGALAEKNGWAGIVVHGCVRDAAELRECKVGVLALATHPRKS 124

Query: 157 YKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELS 197
            K+  GE+ VP+TV GTRI  GEW+YAD DG+LVS T L+
Sbjct: 125 DKRGAGERDVPVTVLGTRIAPGEWIYADDDGVLVSATSLT 164

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BLAST of Lsi06G006940 vs. TrEMBL
Match: A0A0A0LXT5_CUCSA (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis sativus GN=Csa_1G657460 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 2.6e-89
Identity = 165/166 (99.40%), Postives = 166/166 (100.00%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGW+GIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWSGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Lsi06G006940 vs. TrEMBL
Match: B9IQ20_POPTR (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Populus trichocarpa GN=POPTR_0019s08100g PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 1.9e-84
Identity = 155/169 (91.72%), Postives = 162/169 (95.86%), Query Frame = 1


Query: 29  LEEMALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREF 88
           L+ MALVTTAEVCDANPQLIVSGELRAL+PIFQIYGRRQVFSGPVVTLKVFEDNVLIREF
Sbjct: 9   LDSMALVTTAEVCDANPQLIVSGELRALQPIFQIYGRRQVFSGPVVTLKVFEDNVLIREF 68

Query: 89  LEEKGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRA 148
           LEEKGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEI GCDIGVRA
Sbjct: 69  LEEKGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEIMGCDIGVRA 128

Query: 149 LASHPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           LASHPMKA KK +GEKHVPIT+ GTRI DGEWLYADTDGIL+SRTEL+V
Sbjct: 129 LASHPMKANKKGIGEKHVPITIGGTRISDGEWLYADTDGILISRTELAV 177

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BLAST of Lsi06G006940 vs. TrEMBL
Match: A0A067EJI8_CITSI (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Citrus sinensis GN=CISIN_1g031057mg PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 2.5e-84
Identity = 154/166 (92.77%), Postives = 160/166 (96.39%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MALVTTAEVCDANPQLIVSGELRAL+PIFQIYGRRQVFSGP+VTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALQPIFQIYGRRQVFSGPIVTLKVFEDNVLIREFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HPMKA KK +GEKHVPIT+ GTRI DGEWLYADTDGIL+SR ELSV
Sbjct: 121 HPMKANKKGIGEKHVPITIGGTRISDGEWLYADTDGILISRAELSV 166

[Close]

BLAST of Lsi06G006940 vs. TrEMBL
Match: V4SME9_9ROSI (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Citrus clementina GN=CICLE_v10012944mg PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 2.5e-84
Identity = 154/166 (92.77%), Postives = 160/166 (96.39%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MALVTTAEVCDANPQLIVSGELRAL+PIFQIYGRRQVFSGP+VTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALQPIFQIYGRRQVFSGPIVTLKVFEDNVLIREFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HPMKA KK +GEKHVPIT+ GTRI DGEWLYADTDGIL+SR ELSV
Sbjct: 121 HPMKANKKGIGEKHVPITIGGTRISDGEWLYADTDGILISRAELSV 166

[Close]

BLAST of Lsi06G006940 vs. TrEMBL
Match: A0A068TPU4_COFCA (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Coffea canephora GN=GSCOC_T00022026001 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 3.3e-84
Identity = 153/166 (92.17%), Postives = 160/166 (96.39%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MALVTTAEVCDANPQLIVSGELRAL+PIFQIYGRRQVFSGP+VTLKVFEDNVL+REFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALQPIFQIYGRRQVFSGPIVTLKVFEDNVLVREFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HPMKA KK +GEKHVPIT+ GTRI DGEWLYADTDGIL+SR ELSV
Sbjct: 121 HPMKANKKGIGEKHVPITIGGTRICDGEWLYADTDGILISRAELSV 166

[Close]

BLAST of Lsi06G006940 vs. TAIR10
Match: AT5G16450.1 (AT5G16450.1 Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase)

HSP 1 Score: 296.2 bits (757), Expect = 1.5e-80
Identity = 141/166 (84.94%), Postives = 156/166 (93.98%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MA VTTAEVCDAN +LI SG+LRAL+PIFQIYGRRQ+FSGPVVT+KVFEDN LIR+F+EE
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS+RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HP+KA KK +GE+ VP+ +AGTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Lsi06G006940 vs. TAIR10
Match: AT3G02770.1 (AT3G02770.1 Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase)

HSP 1 Score: 290.0 bits (741), Expect = 1.1e-78
Identity = 138/166 (83.13%), Postives = 153/166 (92.17%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MA VTTAEVCDAN ++I SG+LRAL+P+FQIYGRRQ+FSGPVVT+KVFEDN LIR FLEE
Sbjct: 1   MAFVTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGI+VNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HP+KA KK +GE+ V + +AGTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Lsi06G006940 vs. TAIR10
Match: AT5G56260.1 (AT5G56260.1 Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase)

HSP 1 Score: 246.5 bits (628), Expect = 1.4e-65
Identity = 116/166 (69.88%), Postives = 139/166 (83.73%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MA   TAE CD+N +LI +G+LRAL PIF+IYG+R+ FSGP+VTLKVFEDNVL+R  LE 
Sbjct: 1   MAAFATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLET 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KG G VLV+DGGGS RCA++GGN    AQNNGW+GIVVNGCVRDVDEIN CD+GVRAL S
Sbjct: 61  KGEGGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           +P+K+ KK  GEK+VP+ + GT I DGEWLYAD+DGIL+S+TELSV
Sbjct: 121 NPLKSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELSV 166

[Close]

BLAST of Lsi06G006940 vs. NCBI nr
Match: gi|659085949|ref|XP_008443692.1| (PREDICTED: regulator of ribonuclease-like protein 2 [Cucumis melo])

HSP 1 Score: 337.4 bits (864), Expect = 1.7e-89
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Lsi06G006940 vs. NCBI nr
Match: gi|449442669|ref|XP_004139103.1| (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis sativus])

HSP 1 Score: 336.3 bits (861), Expect = 3.7e-89
Identity = 165/166 (99.40%), Postives = 166/166 (100.00%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGW+GIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWSGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Lsi06G006940 vs. NCBI nr
Match: gi|729321083|ref|XP_010533431.1| (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Tarenaya hassleriana])

HSP 1 Score: 321.6 bits (823), Expect = 9.4e-85
Identity = 156/166 (93.98%), Postives = 161/166 (96.99%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MALVTTAEVCDANPQLIVSGELRAL+P+FQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALQPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HPMKA KK +GEKHVPI +AGTRI DGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKANKKGIGEKHVPINIAGTRICDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Lsi06G006940 vs. NCBI nr
Match: gi|747074813|ref|XP_011084414.1| (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Sesamum indicum])

HSP 1 Score: 321.2 bits (822), Expect = 1.2e-84
Identity = 156/166 (93.98%), Postives = 162/166 (97.59%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MALVTTAEVCDANPQLIVSGELRAL+PIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALQPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGI+VNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCVRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HP+KA KK +GEKHVPIT+AGTRI DGEWLYADTDGILVS+TELSV
Sbjct: 121 HPVKANKKGIGEKHVPITIAGTRICDGEWLYADTDGILVSKTELSV 166

[Close]

BLAST of Lsi06G006940 vs. NCBI nr
Match: gi|747059567|ref|XP_011076171.1| (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Sesamum indicum])

HSP 1 Score: 321.2 bits (822), Expect = 1.2e-84
Identity = 156/166 (93.98%), Postives = 162/166 (97.59%), Query Frame = 1


Query: 32  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 91
           MALVTTAEVCDANPQLIVSGELRAL+P+FQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALQPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 92  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 151
           KGNGRVLVVDGGGS RCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 152 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 198
           HP+KA KK +GEKHVPIT+AGTRI DGEWLYADTDGILVS+TELSV
Sbjct: 121 HPVKANKKGIGEKHVPITIAGTRISDGEWLYADTDGILVSKTELSV 166

The following BLAST results are available for this feature:

BLAST of Lsi06G006940 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RRAA2_ARATH	2.6e-79	84.94	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 OS=Arabidopsis thaliana GN...	[more]
RRAA1_ARATH	1.9e-77	83.13	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 1 OS=Arabidopsis thaliana GN...	[more]
RRAA3_ARATH	2.4e-64	69.88	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 OS=Arabidopsis thaliana GN...	[more]
RRAAH_CUPPJ	2.2e-49	53.46	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cupriavidus pinatubonensi...	[more]
RRAAH_BURM1	3.7e-49	56.88	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Burkholderia multivorans ...	[more]

BLAST of Lsi06G006940 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LXT5_CUCSA	2.6e-89	99.40	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis sativus GN=Csa_1G657460 PE...	[more]
B9IQ20_POPTR	1.9e-84	91.72	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Populus trichocarpa GN=POPTR_0019s...	[more]
A0A067EJI8_CITSI	2.5e-84	92.77	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Citrus sinensis GN=CISIN_1g031057m...	[more]
V4SME9_9ROSI	2.5e-84	92.77	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Citrus clementina GN=CICLE_v100129...	[more]
A0A068TPU4_COFCA	3.3e-84	92.17	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Coffea canephora GN=GSCOC_T0002202...	[more]

BLAST of Lsi06G006940 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16450.1	1.5e-80	84.94	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]
AT3G02770.1	1.1e-78	83.13	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]
AT5G56260.1	1.4e-65	69.88	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]

BLAST of Lsi06G006940 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659085949\|ref\|XP_008443692.1\|	1.7e-89	100.00	PREDICTED: regulator of ribonuclease-like protein 2 [Cucumis melo]	[more]
gi\|449442669\|ref\|XP_004139103.1\|	3.7e-89	99.40	PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis sativu...	[more]
gi\|729321083\|ref\|XP_010533431.1\|	9.4e-85	93.98	PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Tarenaya hassl...	[more]
gi\|747074813\|ref\|XP_011084414.1\|	1.2e-84	93.98	PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Sesamum indicu...	[more]
gi\|747059567\|ref\|XP_011076171.1\|	1.2e-84	93.98	PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Sesamum indicu...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0051252	regulation of RNA metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008428	ribonuclease inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR010203	RraA
IPR005493	RraA/RraA-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006525	arginine metabolic process
biological_process	GO:0018874	benzoate metabolic process
biological_process	GO:0032259	methylation
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0006560	proline metabolic process
biological_process	GO:0006090	pyruvate metabolic process
biological_process	GO:0051252	regulation of RNA metabolic process
biological_process	GO:0019915	lipid storage
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0047443	4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0008948	oxaloacetate decarboxylase activity
molecular_function	GO:0008428	ribonuclease inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G006940.1	Lsi06G006940.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	GENE3D	G3DSA:3.50.30.40		coord: 35..195 score: 4.2
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	PFAM	PF03737	Methyltransf_6	coord: 37..190 score: 6.9
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	unknown	SSF89562	RraA-like	coord: 35..195 score: 1.31
IPR010203	Regulator of ribonuclease activity A	TIGRFAMs	TIGR01935	TIGR01935	coord: 37..191 score: 1.4
None	No IPR available	PANTHER	PTHR33254	FAMILY NOT NAMED	coord: 29..195 score: 5.0E
None	No IPR available	PANTHER	PTHR33254:SF4	REGULATOR OF RIBONUCLEASE-LIKE PROTEIN 1-RELATED	coord: 29..195 score: 5.0E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi06G006940	Csa1G657460	Cucumber (Chinese Long) v2	culsiB058
Lsi06G006940	CSPI01G32310	Wild cucumber (PI 183967)	cpilsiB059
Lsi06G006940	Cucsa.356700	Cucumber (Gy14) v1	cgylsiB573
Lsi06G006940	CmaCh10G008340	Cucurbita maxima (Rimu)	cmalsiB073
Lsi06G006940	CmaCh11G009370	Cucurbita maxima (Rimu)	cmalsiB128
Lsi06G006940	CmoCh11G009580	Cucurbita moschata (Rifu)	cmolsiB118
Lsi06G006940	CmoCh10G008640	Cucurbita moschata (Rifu)	cmolsiB059
Lsi06G006940	Cla009552	Watermelon (97103) v1	lsiwmB391
Lsi06G006940	MELO3C010055	Melon (DHL92) v3.5.1	lsimeB398
Lsi06G006940	ClCG03G010660	Watermelon (Charleston Gray)	lsiwcgB361
Lsi06G006940	MELO3C010055.2	Melon (DHL92) v3.6.1	lsimedB420
Lsi06G006940	Cla97C03G061270	Watermelon (97103) v2	lsiwmbB362
Lsi06G006940	Bhi02G001085	Wax gourd	lsiwgoB521
Lsi06G006940	Cp4.1LG18g02490	Cucurbita pepo (Zucchini)	cpelsiB299
Lsi06G006940	Cp4.1LG00g01520	Cucurbita pepo (Zucchini)	cpelsiB017
Lsi06G006940	CsGy1G031220	Cucumber (Gy14) v2	cgyblsiB055
Lsi06G006940	Carg22725	Silver-seed gourd	carlsiB485
Lsi06G006940	Carg17796	Silver-seed gourd	carlsiB886
Lsi06G006940	CsaV3_1G044510	Cucumber (Chinese Long) v3	cuclsiB068

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi06G006940	Wax gourd	lsiwgoB511