Lsi05G004130 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G004130
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Peptidyl-tRNA hydrolase family protein
Location	chr05 : 5213207 .. 5216923 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAAAAAAAAAGCCCCTTCTTCTTCAGTTCTTCCTCTTCAGGGAGGTTTGGGTGTGTTCTTCTTCTTCTTCTACTTCTACGGCTTTAACAACGTCCACCGCTGCTTCTGCTCGAATTCAACTTCCTTTCAACTAAACCATCCAATTTACACGCAGGTTCACTCTTTTATCTTCTCCTTCGCTTTCCTAGATTTTCCTTTCGCCTTTCTCTTTCTTCTCGGTTTTCCCCGGACAAATTCAATGAAATATCATAATTCTTCAAGGGTTTTCTTTTTCCTGCTTTAGGTTTCAATGTTATATGCAACATCCGTCCAAAGTGTTTGCTTATTTTCCAATTCGAGTACTGCCCATTTCCATTCTCGTTCCACAAAGCCTCTGGTCTCGACGACGCTCTCTCTACGAGCTTCATTGCCAGATAATGATAATGGCGTTAAAGTGGAGTATACACCTTGGCTTATTGTTGGACTGGGTAACCCAGGAAACAAGTATCACGGGACTCGGCACAATGTAATCGAACATTCTTTAACTCTGTGTATTAAACCTTTAGAGTGAGTTTGGGATGGAATTTTGATAGTATAGTAGTACTTTTAAACGAAGCAAATTTCCTTTCTAGTGCTTCTCTCTAAAGTGTTCCAAACTCACTCTTAACGTGCATTGACACTTAATAACATTGGTCTCTTTGGCCTCTTGGGGTTTATTATTCAGGTTGGTTTCGAAATGATTGATAGCATTGCTCAAGCGCAGGGTATTTTGATGAACACAATACAATCCAAAGCTCTGATTGGAATAGGTTAGTCTTTTTTTTTCCTCCTTCTATGTTATTGAATTGGGTTTCCATCTTATGTGGATTTGAAGTTCATTGAGTTTCTTGAATGATCAGTACAGGTTCAATTGGAGAAGTACCAATTTTGTTGGCAAAGCCTCAGACTTACATGAATTTCTGCGGAGAATCGGTTTGTCTACTTGTCTCTCTTTTCTTCAATAAGCATGGTACACAGCATACAGTTGAGTGATGGTTTGTTTATTTTTGAAGTGAAAGGATCCAAAGCTTAGTTTGAAGCATAGCATATGAAGCTCACTGAGTTCCTTCTATGACCTTATTATATTCCTATGGATATATCTTTTGTTTTCCTTTGAAACATTGATCAATGTCGCAGTTAGATAAGTATGTGGGGAGGAGTTGTGTATGTTGGATATTTTTTACGCCTTTAGATGTTTTAGTTAACCTTCAATTTTGTCCGGGAGGCATTGTTATGGAGTCATTTATTTGCAATATGTATGATTTGTTTGAAATATGTTAAAATACTCAAAAGTACTCATGGTTTTGAAACAGTTATAATAATATAAAAAAATTACATAATTTCCCTTTTCAGTCTCATTAGTCAAGGTTCTCTTAATGAGCAAAGCATTTGTTTGAGAGATTAGAGATGTAAACTGTTAGAAATAAAATAATATTGGTTTCCTATTTTGGAAGTGTTTAAGGAAGGGGGGGGGGGGGGGGGGGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGGGGGGGGGGGGAGGCCCAAAGTTAACTGTGGTTTGCTCAATGTGAACTAAATCTATGATTGAGTAGGTTTTCATTGTGATGATAGCCTGAAGATACTTAGATTTCTCATTTTTCTAACCTTACTGGTTACGTGTACTTGAAAACTCAATTGACTCCCTGTATTAGATTTTGGATGATGCTACAATATTACAATTACTACCTTATATGGCTATATTGATTTGCACCAATTTATATTATATACATGAAGTTTCATCTCAAAACCAGAGGAATGACCCATCTATTTTATAAACATTGTGAGGTCCCTTAGTTTTTCCAATGGGAGATCCTCAGCAATAACAACATTTTAGTTTTTTAAGGTCACCAGACCAGAAAATTTAGATATCATCCCGTTCACTTTCTAACTCGATGTTTTAGCTCTTAAATATCTCAAGAACCCTGCATATCGATGTTTTAATTATATAGCTGCTGATGCTATGAATCTTTCCTTCATTAATTGAATTATTTTGTCATGATGACTAGATAGTTACTCCCTTCACGTCACTTTCTGTTTTAATTTTTATAGGTTGGACCTCTTGCTGCACATTATCAAATACCTTTACGGCACATCTTGTTGGTATAATTTGCAATATCTCTTAGTAATCCTTATTTGTTTAACGTACAAGATGACTAGATTGTAATGGAAGTATCTGTACATGCTTACCATTGTAGACTTATGATGAAATGAGCTTGCCAAATGGCGTTCTTAGGCTCCAGCCAAAAGGAGGACACGGCCATCACAATGGGTATGTGTATGAAAATATTATTTAGTGTTGGTTTCACTTCTATTTTTGTTCTAGTTTTAATTGGTACCTCATGCTTTCAATTTTTCTAACCTTGTTATAGACCTATGGTAATGTTTGGGAGTACTTTTGATATGACCAAAAGTGCTCATTCTGTTTGGGAGTAACATAAAAGTATATTAGGAAAATGTTAGAAGTATTTAAGGTGCTTCATGAGAAAGTTTCTATAGGGTGCTTTTGGCCCAAGTGCTGGACCAGAAAGCACACTTAAATAAGAATTTAGAAGTGTAGAACTAAAAATAAATTAATTTGTTACTAAGCACCTTCTACTAAAGGCCTTATAATTAAAATATCTTTTTGCTAAAAGCACTCTCAAATCTTTTAGTATAAGCACCTTACTGTATTGTATATTTTATTGGAGCATGTGTTTGAACTTCTTAGACGAGCGTTTTAAGTCATTAAGAAAATTGAAGCTCACTGCAGTAATTCTTGTCGAATACCCACCAACTTCCCATTGAAGACTTGATGAACGTGTTTCATATTAGCATTTTATAGTTGAGTTGATTACACATGAATTTTTCTTCTTTGTTTGCCATACAACTTGGAATGCATGTTCTTCTCTTATTTGCCATTTTATAGTCGAGAAATTTATGGCAGCCTGTTCATAAAAAGCTGTTATTGATTTCATTAGTTATACAGGGTGAAGAGTGTAATGGGACATCTGGATGGTCGACGCGAGTTTCCTCGGCTATGCATCGGTATCCATCTACGTTAACATTAGTTTGAAGATGTTTATCAAGTTTCCACTTCAGGAAGTGAAATAACTGCATTGCTCTTGTTATTACTTTCTAGGAATTGGAAACCCACCTGGGACTATGGACATGAAAGCTTTTCTCCTGCAGAAGTTCAGTTCAGTTGAGCGAAAACAGGTGAGACTGCCTTTGCATTCTAATACAGTTCTATTCTGCATTATGCCATACGTCGCAGCCATACTCGATAACTTTGAAATTTTACGTGTTTATTTTGAACTATTAATTGATTTTTATGATGTAGCTGGTGGTAGAGCACCATTGCGTTGTACAAATTTTTATGACTATTGTTTGCTCAACCCAACAGATCGATGCAGCCCTTGAACAAGGAGTCGAGGCAGTGAGAACCCTTGCACTGAATGGATTTAGCCAAAGCGTGAATAGATTCAATTTGACCCAGAAATACAAGTATCACAAGGTTTGA

mRNA sequence

AAAAAAAAAAAAAAAAGCCCCTTCTTCTTCAGTTCTTCCTCTTCAGGGAGGTTTGGGTGTGTTCTTCTTCTTCTTCTACTTCTACGGCTTTAACAACGTCCACCGCTGCTTCTGCTCGAATTCAACTTCCTTTCAACTAAACCATCCAATTTACACGCAGGTTTCAATGTTATATGCAACATCCGTCCAAAGTGTTTGCTTATTTTCCAATTCGAGTACTGCCCATTTCCATTCTCGTTCCACAAAGCCTCTGGTCTCGACGACGCTCTCTCTACGAGCTTCATTGCCAGATAATGATAATGGCGTTAAAGTGGAGTATACACCTTGGCTTATTGTTGGACTGGGTAACCCAGGAAACAAGTATCACGGGACTCGGCACAATGTTGGTTTCGAAATGATTGATAGCATTGCTCAAGCGCAGGGTATTTTGATGAACACAATACAATCCAAAGCTCTGATTGGAATAGGTTCAATTGGAGAAGTACCAATTTTGTTGGCAAAGCCTCAGACTTACATGAATTTCTGCGGAGAATCGGTTGGACCTCTTGCTGCACATTATCAAATACCTTTACGGCACATCTTGTTGACTTATGATGAAATGAGCTTGCCAAATGGCGTTCTTAGGCTCCAGCCAAAAGGAGGACACGGCCATCACAATGGGGTGAAGAGTGTAATGGGACATCTGGATGGTCGACGCGAGTTTCCTCGGCTATGCATCGGAATTGGAAACCCACCTGGGACTATGGACATGAAAGCTTTTCTCCTGCAGAAGTTCAGTTCAGTTGAGCGAAAACAGCTGGTGGTAGAGCACCATTGCGTTGTACAAATTTTTATGACTATTGTTTGCTCAACCCAACAGATCGATGCAGCCCTTGAACAAGGAGTCGAGGCAGTGAGAACCCTTGCACTGAATGGATTTAGCCAAAGCGTGAATAGATTCAATTTGACCCAGAAATACAAGTATCACAAGGTTTGA

Coding sequence (CDS)

ATGTTATATGCAACATCCGTCCAAAGTGTTTGCTTATTTTCCAATTCGAGTACTGCCCATTTCCATTCTCGTTCCACAAAGCCTCTGGTCTCGACGACGCTCTCTCTACGAGCTTCATTGCCAGATAATGATAATGGCGTTAAAGTGGAGTATACACCTTGGCTTATTGTTGGACTGGGTAACCCAGGAAACAAGTATCACGGGACTCGGCACAATGTTGGTTTCGAAATGATTGATAGCATTGCTCAAGCGCAGGGTATTTTGATGAACACAATACAATCCAAAGCTCTGATTGGAATAGGTTCAATTGGAGAAGTACCAATTTTGTTGGCAAAGCCTCAGACTTACATGAATTTCTGCGGAGAATCGGTTGGACCTCTTGCTGCACATTATCAAATACCTTTACGGCACATCTTGTTGACTTATGATGAAATGAGCTTGCCAAATGGCGTTCTTAGGCTCCAGCCAAAAGGAGGACACGGCCATCACAATGGGGTGAAGAGTGTAATGGGACATCTGGATGGTCGACGCGAGTTTCCTCGGCTATGCATCGGAATTGGAAACCCACCTGGGACTATGGACATGAAAGCTTTTCTCCTGCAGAAGTTCAGTTCAGTTGAGCGAAAACAGCTGGTGGTAGAGCACCATTGCGTTGTACAAATTTTTATGACTATTGTTTGCTCAACCCAACAGATCGATGCAGCCCTTGAACAAGGAGTCGAGGCAGTGAGAACCCTTGCACTGAATGGATTTAGCCAAAGCGTGAATAGATTCAATTTGACCCAGAAATACAAGTATCACAAGGTTTGA

Protein sequence

MLYATSVQSVCLFSNSSTAHFHSRSTKPLVSTTLSLRASLPDNDNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQGVEAVRTLALNGFSQSVNRFNLTQKYKYHKV

Homology

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BLAST of Lsi05G004130 vs. Swiss-Prot
Match: CRS2B_ARATH (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana GN=CRS2B PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 5.7e-93
Identity = 165/226 (73.01%), Postives = 186/226 (82.30%), Query Frame = 1


Query: 44  DNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSI 103
           D+  KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMID +A+ +G+LMNTIQSKALIGIG+I
Sbjct: 36  DDRFKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAI 95

Query: 104 GEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHH 163
            +VPILLAKPQTYMNF GESVG LA+HY++PLRHIL+ YDEM+LPNGVLRLQPKGG G+H
Sbjct: 96  EDVPILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYH 155

Query: 164 NGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFM 223
           NGVKSVMGHLDGRR FPRL IGIG PPG MDMKAFLLQKFS +E+K              
Sbjct: 156 NGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQK-------------- 215

Query: 224 TIVCSTQQIDAALEQGVEAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
                  QI+ ALEQG EAV+TL LNGF+Q ++RFNL QKYK+HKV
Sbjct: 216 -------QIEEALEQGSEAVKTLVLNGFNQGISRFNLVQKYKFHKV 240

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BLAST of Lsi05G004130 vs. Swiss-Prot
Match: CRS2A_ARATH (Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Arabidopsis thaliana GN=CRS2A PE=2 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 7.3e-88
Identity = 163/236 (69.07%), Postives = 183/236 (77.54%), Query Frame = 1


Query: 35  SLRASLPDNDNGV-KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQ 94
           SLRASLP +DN + K EYTPWLIVGLGNPG KY+GTRHN+GFEMID IA+A  I MNTIQ
Sbjct: 32  SLRASLPVSDNKLLKFEYTPWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQ 91

Query: 95  SKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLR 154
           SKAL+GIGS+GEVPILL KPQ YMNF GESVGPLAA+YQIPLRHIL+ YD+M L NGVLR
Sbjct: 92  SKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLR 151

Query: 155 LQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVV 214
           LQPKGGH  HNG+K+V  HL+G R +PRL IGIGNPPG MDMKAFLLQKFS +ERKQ+  
Sbjct: 152 LQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQM-- 211

Query: 215 EHHCVVQIFMTIVCSTQQIDAALEQGVEAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
                              D  LEQGVE V+TL   GFS S++RFNL QKYK+HKV
Sbjct: 212 -------------------DEGLEQGVEGVKTLVEEGFSDSISRFNLGQKYKFHKV 246

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BLAST of Lsi05G004130 vs. Swiss-Prot
Match: CRS2_MAIZE (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays GN=CRS2 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 8.0e-87
Identity = 165/254 (64.96%), Postives = 191/254 (75.20%), Query Frame = 1


Query: 16  SSTAHFHSRSTKPLVSTTLSLRASLPDNDNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGF 75
           S TA  H R  +P   T +S   S+PD   G  VEYTPWLI GLGNPGNKY+GTRHNVGF
Sbjct: 29  SLTAPLH-RIRRPRPFTVVS---SVPDPAAG-PVEYTPWLIAGLGNPGNKYYGTRHNVGF 88

Query: 76  EMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPL 135
           EM+D IA  +GI MNTIQSK+L+GIGSIGEVP+L+ KPQ+YMN+ GE++GPLAA+YQ+PL
Sbjct: 89  EMVDRIAAEEGITMNTIQSKSLLGIGSIGEVPVLVVKPQSYMNYSGEAIGPLAAYYQVPL 148

Query: 136 RHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDM 195
           RHILL YD+ SLPNGVLRLQ KGGHG HNG+++V+ HLDGRREFPRL IGIG+PPG MD 
Sbjct: 149 RHILLIYDDTSLPNGVLRLQKKGGHGRHNGLQNVIEHLDGRREFPRLSIGIGSPPGKMDP 208

Query: 196 KAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQGVEAVRTLALNGFSQSV 255
           +AFLLQKFSS ER                      QID ALEQGV+AVRTL L GFS S 
Sbjct: 209 RAFLLQKFSSEER---------------------VQIDTALEQGVDAVRTLVLKGFSGST 256

Query: 256 NRFNLTQKYKYHKV 270
            RFNL QKYK+H+V
Sbjct: 269 ERFNLVQKYKFHRV 256

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BLAST of Lsi05G004130 vs. Swiss-Prot
Match: CRS2_ORYSJ (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza sativa subsp. japonica GN=Os01g0132800 PE=2 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 3.7e-84
Identity = 159/246 (64.63%), Postives = 183/246 (74.39%), Query Frame = 1


Query: 28  PLVSTTLSLR----ASLPDNDNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQ 87
           PL  T L  R    AS+PD       EYTPWLI GLGNPG+KYHGTRHNVGFEM+D IA+
Sbjct: 36  PLRRTRLRRRIAVVASVPD-PAARPAEYTPWLIAGLGNPGSKYHGTRHNVGFEMVDRIAR 95

Query: 88  AQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLTYD 147
            +GI MNTIQSK+L+GIGSIGEVP+LL KPQ+Y+N+ GE++GPLAA+YQ+PLRHIL+ YD
Sbjct: 96  DEGITMNTIQSKSLLGIGSIGEVPVLLVKPQSYINYSGEAIGPLAAYYQVPLRHILVMYD 155

Query: 148 EMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKF 207
           EMSLPNGVLRLQ KGGHG HNG+++VM  LD  RE PRL IGIG+PPG MD +AFLLQKF
Sbjct: 156 EMSLPNGVLRLQRKGGHGRHNGLQNVMECLDSSRELPRLSIGIGSPPGKMDTRAFLLQKF 215

Query: 208 SSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQGVEAVRTLALNGFSQSVNRFNLTQK 267
           SS ER                      QID ALEQGV+AVRTL L GFS S+ RFNL QK
Sbjct: 216 SSEER---------------------LQIDTALEQGVDAVRTLVLKGFSGSIERFNLVQK 259

Query: 268 YKYHKV 270
           YK+H V
Sbjct: 276 YKFHSV 259

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BLAST of Lsi05G004130 vs. Swiss-Prot
Match: PTHM_ARATH (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.1e-62
Identity = 116/218 (53.21%), Postives = 149/218 (68.35%), Query Frame = 1


Query: 51  YTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILL 110
           + PWL +GLGNPG+KY GTRHN+GFEMID  A++ GI MN +  KA++G G + ++P++L
Sbjct: 16  HRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVIL 75

Query: 111 AKPQTYMNFCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVM 170
           AKPQTYMN  GES GPLAA+Y++PL  +L+ +D+M LP GVLRLQ KGGHG HNG+KSVM
Sbjct: 76  AKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVM 135

Query: 171 GHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQ 230
            H  G REF RL IGIG PPG MD KAFLLQKFS   R                     +
Sbjct: 136 NHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPAR---------------------E 195

Query: 231 QIDAALEQGVEAVRTLALNGFSQSVNRFNLTQKYKYHK 269
           ++D AL +GV+A++ +    F +S   FN+ QKYK+ K
Sbjct: 196 RMDKALAEGVDALKLVLAKDFGESWRLFNVEQKYKHLK 212

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BLAST of Lsi05G004130 vs. TrEMBL
Match: A0A0A0LCC6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G257600 PE=3 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 1.8e-125
Identity = 231/270 (85.56%), Postives = 239/270 (88.52%), Query Frame = 1


Query: 1   MLYATSVQSVCLFSNSSTAHFHSRSTKPLVSTTLSLRASLPDND-NGVKVEYTPWLIVGL 60
           ML+A S QSV LFSNSS+AHFH  STKPLVST LS+RAS PD+D NG+KVEYTPWLIVGL
Sbjct: 1   MLFAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDSNGLKVEYTPWLIVGL 60

Query: 61  GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 120
           GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF
Sbjct: 61  GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 120

Query: 121 CGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF 180
           CGESVGPLAAHYQIPLRH+LLTYDEM+LPNGVLRLQPK GHGHHNGVKSVMGHLDGRREF
Sbjct: 121 CGESVGPLAAHYQIPLRHVLLTYDEMNLPNGVLRLQPKAGHGHHNGVKSVMGHLDGRREF 180

Query: 181 PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQG 240
           PRLCIGIGNPPGTMDMKAFLLQKFSSVERK                     QIDAALEQG
Sbjct: 181 PRLCIGIGNPPGTMDMKAFLLQKFSSVERK---------------------QIDAALEQG 240

Query: 241 VEAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
           VEAVRTLALNGFSQ VNRFNL+QKYKYHKV
Sbjct: 241 VEAVRTLALNGFSQRVNRFNLSQKYKYHKV 249

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BLAST of Lsi05G004130 vs. TrEMBL
Match: B9H311_POPTR (Peptidyl-tRNA hydrolase family protein OS=Populus trichocarpa GN=POPTR_0004s09730g PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.2e-105
Identity = 198/269 (73.61%), Postives = 214/269 (79.55%), Query Frame = 1


Query: 1   MLYATSVQSVCLFSNSSTAHFHSRSTKPLVSTTLSLRASLPDNDNGVKVEYTPWLIVGLG 60
           MLYA S    C+ S   ++ FH R+   LVS  L +RASLPDN NGVKVEYTPWLIVGLG
Sbjct: 1   MLYAVSTPRTCI-SCPRSSRFHRRN---LVSVRLCVRASLPDNANGVKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPG KYHGTRHNVGFEMID IAQA+G+LMNTIQSKALIGIG IGEVPILLAKPQ YMNF 
Sbjct: 61  NPGTKYHGTRHNVGFEMIDRIAQAEGVLMNTIQSKALIGIGCIGEVPILLAKPQAYMNFS 120

Query: 121 GESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLAAHY+IPLRHILL YDEM+LPNG+LR+QPKGGHGHHNGVKSVM HLDG REFP
Sbjct: 121 GESVGPLAAHYKIPLRHILLIYDEMNLPNGILRVQPKGGHGHHNGVKSVMDHLDGCREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQGV 240
           RLCIGIGNPPGTMDMKAFLLQKFS  ER                     +QIDA+LEQGV
Sbjct: 181 RLCIGIGNPPGTMDMKAFLLQKFSPTER---------------------EQIDASLEQGV 240

Query: 241 EAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
           EA+RTL L GF+  + RFNL QKYKYHKV
Sbjct: 241 EAMRTLILGGFTNKITRFNLGQKYKYHKV 244

[Close]

BLAST of Lsi05G004130 vs. TrEMBL
Match: A0A061EH51_THECC (Peptidyl-tRNA hydrolase family protein isoform 1 OS=Theobroma cacao GN=TCM_019422 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.2e-105
Identity = 197/269 (73.23%), Postives = 215/269 (79.93%), Query Frame = 1


Query: 1   MLYATSVQSVCLFSNSSTAHFHSRSTKPLVSTTLSLRASLPDNDNGVKVEYTPWLIVGLG 60
           ML+A SV + C+    S       S +   ST L +RASLPDN++G KVEYTPWLIVGLG
Sbjct: 1   MLHAISVPNTCIPYPRSRCF----SQRNRGSTRLCVRASLPDNNDGFKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGFEMID I+Q +GI +NTIQSKALIG+G+IGEVPILLAKPQ YMNFC
Sbjct: 61  NPGNKYHGTRHNVGFEMIDQISQVEGIALNTIQSKALIGLGAIGEVPILLAKPQAYMNFC 120

Query: 121 GESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLAA+YQ+PLRHILL YDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDG REFP
Sbjct: 121 GESVGPLAAYYQVPLRHILLIYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGCREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQGV 240
           RLCIGIGNPPG+MDMKA+LLQKFSSVER                      QIDAALEQGV
Sbjct: 181 RLCIGIGNPPGSMDMKAYLLQKFSSVER---------------------NQIDAALEQGV 240

Query: 241 EAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
           EAVRT+ LNGFS  + RFNL QKYKYHKV
Sbjct: 241 EAVRTIVLNGFSHKITRFNLGQKYKYHKV 244

[Close]

BLAST of Lsi05G004130 vs. TrEMBL
Match: A0A061EHT4_THECC (Peptidyl-tRNA hydrolase family protein isoform 3 (Fragment) OS=Theobroma cacao GN=TCM_019422 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 2.7e-105
Identity = 189/239 (79.08%), Postives = 203/239 (84.94%), Query Frame = 1


Query: 31  STTLSLRASLPDNDNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMN 90
           ST L +RASLPDN++G KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMID I+Q +GI +N
Sbjct: 5   STRLCVRASLPDNNDGFKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDQISQVEGIALN 64

Query: 91  TIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLTYDEMSLPNG 150
           TIQSKALIG+G+IGEVPILLAKPQ YMNFCGESVGPLAA+YQ+PLRHILL YDEMSLPNG
Sbjct: 65  TIQSKALIGLGAIGEVPILLAKPQAYMNFCGESVGPLAAYYQVPLRHILLIYDEMSLPNG 124

Query: 151 VLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQ 210
           VLRLQPKGGHGHHNGVKSVMGHLDG REFPRLCIGIGNPPG+MDMKA+LLQKFSSVER  
Sbjct: 125 VLRLQPKGGHGHHNGVKSVMGHLDGCREFPRLCIGIGNPPGSMDMKAYLLQKFSSVER-- 184

Query: 211 LVVEHHCVVQIFMTIVCSTQQIDAALEQGVEAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
                               QIDAALEQGVEAVRT+ LNGFS  + RFNL QKYKYHKV
Sbjct: 185 -------------------NQIDAALEQGVEAVRTIVLNGFSHKITRFNLGQKYKYHKV 222

[Close]

BLAST of Lsi05G004130 vs. TrEMBL
Match: M5WYR9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010513mg PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 1.5e-103
Identity = 184/240 (76.67%), Postives = 204/240 (85.00%), Query Frame = 1


Query: 30  VSTTLSLRASLPDNDNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILM 89
           V T+  ++ASLP+N+NG KVEYTPWLIVGLGNPGNKYHGTRHN+GFEMIDSIA+ QG++M
Sbjct: 29  VPTSFCVQASLPENNNGAKVEYTPWLIVGLGNPGNKYHGTRHNIGFEMIDSIAKTQGVVM 88

Query: 90  NTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLTYDEMSLPN 149
           NTIQSKAL+GIGSIGEVPILLAKPQ YMN+ GESVGPLAA+YQ+PLRHILL YDEMSLPN
Sbjct: 89  NTIQSKALVGIGSIGEVPILLAKPQAYMNYSGESVGPLAAYYQVPLRHILLVYDEMSLPN 148

Query: 150 GVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERK 209
           GVLR+QPKGGHG+HNGVKSVMGHLDG REFPRLCIGIGNPPGTMDMKA+LLQ+FS+VER 
Sbjct: 149 GVLRIQPKGGHGYHNGVKSVMGHLDGCREFPRLCIGIGNPPGTMDMKAYLLQRFSTVER- 208

Query: 210 QLVVEHHCVVQIFMTIVCSTQQIDAALEQGVEAVRTLALNGFSQSVNRFNLTQKYKYHKV 269
                                QIDAAL+QGVEAVRTL LNGF QS+  FNL QKYKYHKV
Sbjct: 209 --------------------HQIDAALDQGVEAVRTLVLNGFDQSITSFNLRQKYKYHKV 247

[Close]

BLAST of Lsi05G004130 vs. TAIR10
Match: AT5G16140.1 (AT5G16140.1 Peptidyl-tRNA hydrolase family protein)

HSP 1 Score: 342.0 bits (876), Expect = 3.2e-94
Identity = 165/226 (73.01%), Postives = 186/226 (82.30%), Query Frame = 1


Query: 44  DNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSI 103
           D+  KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMID +A+ +G+LMNTIQSKALIGIG+I
Sbjct: 36  DDRFKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAI 95

Query: 104 GEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHH 163
            +VPILLAKPQTYMNF GESVG LA+HY++PLRHIL+ YDEM+LPNGVLRLQPKGG G+H
Sbjct: 96  EDVPILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYH 155

Query: 164 NGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFM 223
           NGVKSVMGHLDGRR FPRL IGIG PPG MDMKAFLLQKFS +E+K              
Sbjct: 156 NGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQK-------------- 215

Query: 224 TIVCSTQQIDAALEQGVEAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
                  QI+ ALEQG EAV+TL LNGF+Q ++RFNL QKYK+HKV
Sbjct: 216 -------QIEEALEQGSEAVKTLVLNGFNQGISRFNLVQKYKFHKV 240

[Close]

BLAST of Lsi05G004130 vs. TAIR10
Match: AT5G38290.2 (AT5G38290.2 Peptidyl-tRNA hydrolase family protein)

HSP 1 Score: 325.1 bits (832), Expect = 4.1e-89
Identity = 163/236 (69.07%), Postives = 183/236 (77.54%), Query Frame = 1


Query: 35  SLRASLPDNDNGV-KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQ 94
           SLRASLP +DN + K EYTPWLIVGLGNPG KY+GTRHN+GFEMID IA+A  I MNTIQ
Sbjct: 36  SLRASLPVSDNKLLKFEYTPWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQ 95

Query: 95  SKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLR 154
           SKAL+GIGS+GEVPILL KPQ YMNF GESVGPLAA+YQIPLRHIL+ YD+M L NGVLR
Sbjct: 96  SKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLR 155

Query: 155 LQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVV 214
           LQPKGGH  HNG+K+V  HL+G R +PRL IGIGNPPG MDMKAFLLQKFS +ERKQ+  
Sbjct: 156 LQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQM-- 215

Query: 215 EHHCVVQIFMTIVCSTQQIDAALEQGVEAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
                              D  LEQGVE V+TL   GFS S++RFNL QKYK+HKV
Sbjct: 216 -------------------DEGLEQGVEGVKTLVEEGFSDSISRFNLGQKYKFHKV 250

[Close]

BLAST of Lsi05G004130 vs. TAIR10
Match: AT5G19830.1 (AT5G19830.1 Peptidyl-tRNA hydrolase family protein)

HSP 1 Score: 240.0 bits (611), Expect = 1.7e-63
Identity = 116/218 (53.21%), Postives = 149/218 (68.35%), Query Frame = 1


Query: 51  YTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILL 110
           + PWL +GLGNPG+KY GTRHN+GFEMID  A++ GI MN +  KA++G G + ++P++L
Sbjct: 16  HRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVIL 75

Query: 111 AKPQTYMNFCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVM 170
           AKPQTYMN  GES GPLAA+Y++PL  +L+ +D+M LP GVLRLQ KGGHG HNG+KSVM
Sbjct: 76  AKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVM 135

Query: 171 GHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQ 230
            H  G REF RL IGIG PPG MD KAFLLQKFS   R                     +
Sbjct: 136 NHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPAR---------------------E 195

Query: 231 QIDAALEQGVEAVRTLALNGFSQSVNRFNLTQKYKYHK 269
           ++D AL +GV+A++ +    F +S   FN+ QKYK+ K
Sbjct: 196 RMDKALAEGVDALKLVLAKDFGESWRLFNVEQKYKHLK 212

[Close]

BLAST of Lsi05G004130 vs. TAIR10
Match: AT1G18440.1 (AT1G18440.1 Peptidyl-tRNA hydrolase family protein)

HSP 1 Score: 232.3 bits (591), Expect = 3.6e-61
Identity = 119/253 (47.04%), Postives = 158/253 (62.45%), Query Frame = 1


Query: 7   VQSVCLFSNSSTAHFHSRSTKPLVSTTLSLRASLPDNDNGVKVEYTPWLIVGLGNPGNKY 66
           + S CL S+S +  F S S      +   L  +LP +         PWLIVGLGNPG KY
Sbjct: 50  LMSQCLSSSSQSLSFSSNSFSSQPES--ELLQALPSSKPKSPPLQLPWLIVGLGNPGKKY 109

Query: 67  HGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGP 126
            GTRHNVGFEM+D++A A+GI MNT+  KAL G G IG +PI+LAKPQT+MN  GESVG 
Sbjct: 110 QGTRHNVGFEMVDALADAEGISMNTVNFKALFGKGVIGNIPIMLAKPQTFMNLSGESVGQ 169

Query: 127 LAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGI 186
           + + Y+IPL+ +L+ YD++ LP G LRL PKGGHG HNG++S++  L G R+FPRL IGI
Sbjct: 170 IVSFYKIPLKQVLVVYDDLDLPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGI 229

Query: 187 GNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQGVEAVRTL 246
           G PPG MD   F+L++F+  E+++L                     D   + G+EA+R L
Sbjct: 230 GRPPGKMDTANFVLRQFNRQEQEEL---------------------DHTFQTGLEAIRIL 279

Query: 247 ALNGFSQSVNRFN 260
            L GF++S    N
Sbjct: 290 LLEGFNKSATFVN 279

[Close]

BLAST of Lsi05G004130 vs. NCBI nr
Match: gi|659123230|ref|XP_008461557.1| (PREDICTED: chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic [Cucumis melo])

HSP 1 Score: 468.0 bits (1203), Expect = 1.1e-128
Identity = 238/271 (87.82%), Postives = 242/271 (89.30%), Query Frame = 1


Query: 1   MLYATSVQSVCLFSNSSTAHFHSRSTKPLVSTTLSLRASLPDND--NGVKVEYTPWLIVG 60
           MLYA S QSV LFSNSSTAH+HS STKPLVST LS+RASLPD+D  NGVKVEYTPWLIVG
Sbjct: 1   MLYAASFQSVSLFSNSSTAHYHSPSTKPLVSTRLSVRASLPDSDNNNGVKVEYTPWLIVG 60

Query: 61  LGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMN 120
           LGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMN
Sbjct: 61  LGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMN 120

Query: 121 FCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRRE 180
           FCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRRE
Sbjct: 121 FCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRRE 180

Query: 181 FPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQ 240
           FPRLCIGIGNPPGTMDMKAFLLQKFSSVERK                     QIDAALEQ
Sbjct: 181 FPRLCIGIGNPPGTMDMKAFLLQKFSSVERK---------------------QIDAALEQ 240

Query: 241 GVEAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
           GVEAVRTLALNGFSQ VNRFNL+QKYKYHKV
Sbjct: 241 GVEAVRTLALNGFSQRVNRFNLSQKYKYHKV 250

[Close]

BLAST of Lsi05G004130 vs. NCBI nr
Match: gi|449455782|ref|XP_004145630.1| (PREDICTED: chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic [Cucumis sativus])

HSP 1 Score: 456.8 bits (1174), Expect = 2.6e-125
Identity = 231/270 (85.56%), Postives = 239/270 (88.52%), Query Frame = 1


Query: 1   MLYATSVQSVCLFSNSSTAHFHSRSTKPLVSTTLSLRASLPDND-NGVKVEYTPWLIVGL 60
           ML+A S QSV LFSNSS+AHFH  STKPLVST LS+RAS PD+D NG+KVEYTPWLIVGL
Sbjct: 1   MLFAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDSNGLKVEYTPWLIVGL 60

Query: 61  GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 120
           GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF
Sbjct: 61  GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 120

Query: 121 CGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF 180
           CGESVGPLAAHYQIPLRH+LLTYDEM+LPNGVLRLQPK GHGHHNGVKSVMGHLDGRREF
Sbjct: 121 CGESVGPLAAHYQIPLRHVLLTYDEMNLPNGVLRLQPKAGHGHHNGVKSVMGHLDGRREF 180

Query: 181 PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQG 240
           PRLCIGIGNPPGTMDMKAFLLQKFSSVERK                     QIDAALEQG
Sbjct: 181 PRLCIGIGNPPGTMDMKAFLLQKFSSVERK---------------------QIDAALEQG 240

Query: 241 VEAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
           VEAVRTLALNGFSQ VNRFNL+QKYKYHKV
Sbjct: 241 VEAVRTLALNGFSQRVNRFNLSQKYKYHKV 249

[Close]

BLAST of Lsi05G004130 vs. NCBI nr
Match: gi|1009128818|ref|XP_015881440.1| (PREDICTED: chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 394.8 bits (1013), Expect = 1.2e-106
Identity = 202/271 (74.54%), Postives = 217/271 (80.07%), Query Frame = 1


Query: 1   MLYATSVQSVCLF--SNSSTAHFHSRSTKPLVSTTLSLRASLPDNDNGVKVEYTPWLIVG 60
           M YA S     +F      + HF  +  K  +ST+  + ASLPD++NGVK EYTPWLIVG
Sbjct: 1   MWYAVSFSHSSIFYPRRPWSVHFLQKQ-KQSISTSCRISASLPDHNNGVKAEYTPWLIVG 60

Query: 61  LGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMN 120
           LGNPGNKYHGTRHNVGFEMIDSI+Q+QGI+MNTIQSKALIGIGSI EVPILL KPQ+YMN
Sbjct: 61  LGNPGNKYHGTRHNVGFEMIDSISQSQGIVMNTIQSKALIGIGSIEEVPILLVKPQSYMN 120

Query: 121 FCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRRE 180
           F GESVGPLAA+YQ+PLRHILL YDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRRE
Sbjct: 121 FSGESVGPLAAYYQVPLRHILLIYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRRE 180

Query: 181 FPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQ 240
           FPRLCIGIGNPPGTMDMKAFLLQKFS+VER                      QIDAALEQ
Sbjct: 181 FPRLCIGIGNPPGTMDMKAFLLQKFSTVER---------------------HQIDAALEQ 240

Query: 241 GVEAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
           GVEAVRTL LNGFS SV RFNL QKYKYHKV
Sbjct: 241 GVEAVRTLVLNGFSNSVTRFNLGQKYKYHKV 249

[Close]

BLAST of Lsi05G004130 vs. NCBI nr
Match: gi|224079329|ref|XP_002305823.1| (peptidyl-tRNA hydrolase family protein [Populus trichocarpa])

HSP 1 Score: 391.0 bits (1003), Expect = 1.7e-105
Identity = 198/269 (73.61%), Postives = 214/269 (79.55%), Query Frame = 1


Query: 1   MLYATSVQSVCLFSNSSTAHFHSRSTKPLVSTTLSLRASLPDNDNGVKVEYTPWLIVGLG 60
           MLYA S    C+ S   ++ FH R+   LVS  L +RASLPDN NGVKVEYTPWLIVGLG
Sbjct: 1   MLYAVSTPRTCI-SCPRSSRFHRRN---LVSVRLCVRASLPDNANGVKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPG KYHGTRHNVGFEMID IAQA+G+LMNTIQSKALIGIG IGEVPILLAKPQ YMNF 
Sbjct: 61  NPGTKYHGTRHNVGFEMIDRIAQAEGVLMNTIQSKALIGIGCIGEVPILLAKPQAYMNFS 120

Query: 121 GESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLAAHY+IPLRHILL YDEM+LPNG+LR+QPKGGHGHHNGVKSVM HLDG REFP
Sbjct: 121 GESVGPLAAHYKIPLRHILLIYDEMNLPNGILRVQPKGGHGHHNGVKSVMDHLDGCREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQGV 240
           RLCIGIGNPPGTMDMKAFLLQKFS  ER                     +QIDA+LEQGV
Sbjct: 181 RLCIGIGNPPGTMDMKAFLLQKFSPTER---------------------EQIDASLEQGV 240

Query: 241 EAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
           EA+RTL L GF+  + RFNL QKYKYHKV
Sbjct: 241 EAMRTLILGGFTNKITRFNLGQKYKYHKV 244

[Close]

BLAST of Lsi05G004130 vs. NCBI nr
Match: gi|590652761|ref|XP_007033235.1| (Peptidyl-tRNA hydrolase family protein isoform 1 [Theobroma cacao])

HSP 1 Score: 391.0 bits (1003), Expect = 1.7e-105
Identity = 197/269 (73.23%), Postives = 215/269 (79.93%), Query Frame = 1


Query: 1   MLYATSVQSVCLFSNSSTAHFHSRSTKPLVSTTLSLRASLPDNDNGVKVEYTPWLIVGLG 60
           ML+A SV + C+    S       S +   ST L +RASLPDN++G KVEYTPWLIVGLG
Sbjct: 1   MLHAISVPNTCIPYPRSRCF----SQRNRGSTRLCVRASLPDNNDGFKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGFEMID I+Q +GI +NTIQSKALIG+G+IGEVPILLAKPQ YMNFC
Sbjct: 61  NPGNKYHGTRHNVGFEMIDQISQVEGIALNTIQSKALIGLGAIGEVPILLAKPQAYMNFC 120

Query: 121 GESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLAA+YQ+PLRHILL YDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDG REFP
Sbjct: 121 GESVGPLAAYYQVPLRHILLIYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGCREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQLVVEHHCVVQIFMTIVCSTQQIDAALEQGV 240
           RLCIGIGNPPG+MDMKA+LLQKFSSVER                      QIDAALEQGV
Sbjct: 181 RLCIGIGNPPGSMDMKAYLLQKFSSVER---------------------NQIDAALEQGV 240

Query: 241 EAVRTLALNGFSQSVNRFNLTQKYKYHKV 270
           EAVRT+ LNGFS  + RFNL QKYKYHKV
Sbjct: 241 EAVRTIVLNGFSHKITRFNLGQKYKYHKV 244

The following BLAST results are available for this feature:

BLAST of Lsi05G004130 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CRS2B_ARATH	5.7e-93	73.01	Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Ara...	[more]
CRS2A_ARATH	7.3e-88	69.07	Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Ara...	[more]
CRS2_MAIZE	8.0e-87	64.96	Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea m...	[more]
CRS2_ORYSJ	3.7e-84	64.63	Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza...	[more]
PTHM_ARATH	3.1e-62	53.21	Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana GN=At5g19830 PE=2...	[more]

BLAST of Lsi05G004130 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCC6_CUCSA	1.8e-125	85.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G257600 PE=3 SV=1	[more]
B9H311_POPTR	1.2e-105	73.61	Peptidyl-tRNA hydrolase family protein OS=Populus trichocarpa GN=POPTR_0004s0973...	[more]
A0A061EH51_THECC	1.2e-105	73.23	Peptidyl-tRNA hydrolase family protein isoform 1 OS=Theobroma cacao GN=TCM_01942...	[more]
A0A061EHT4_THECC	2.7e-105	79.08	Peptidyl-tRNA hydrolase family protein isoform 3 (Fragment) OS=Theobroma cacao G...	[more]
M5WYR9_PRUPE	1.5e-103	76.67	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010513mg PE=3 SV=1	[more]

BLAST of Lsi05G004130 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16140.1	3.2e-94	73.01	Peptidyl-tRNA hydrolase family protein	[more]
AT5G38290.2	4.1e-89	69.07	Peptidyl-tRNA hydrolase family protein	[more]
AT5G19830.1	1.7e-63	53.21	Peptidyl-tRNA hydrolase family protein	[more]
AT1G18440.1	3.6e-61	47.04	Peptidyl-tRNA hydrolase family protein	[more]

BLAST of Lsi05G004130 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659123230\|ref\|XP_008461557.1\|	1.1e-128	87.82	PREDICTED: chloroplastic group IIB intron splicing facilitator CRS2-B, chloropla...	[more]
gi\|449455782\|ref\|XP_004145630.1\|	2.6e-125	85.56	PREDICTED: chloroplastic group IIB intron splicing facilitator CRS2-B, chloropla...	[more]
gi\|1009128818\|ref\|XP_015881440.1\|	1.2e-106	74.54	PREDICTED: chloroplastic group IIB intron splicing facilitator CRS2-B, chloropla...	[more]
gi\|224079329\|ref\|XP_002305823.1\|	1.7e-105	73.61	peptidyl-tRNA hydrolase family protein [Populus trichocarpa]	[more]
gi\|590652761\|ref\|XP_007033235.1\|	1.7e-105	73.23	Peptidyl-tRNA hydrolase family protein isoform 1 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004045	aminoacyl-tRNA hydrolase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR018171	Pept_tRNA_hydro_CS
IPR001328	Pept_tRNA_hydro

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032543	mitochondrial translation
biological_process	GO:0008152	metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G004130.1	Lsi05G004130.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001328	Peptidyl-tRNA hydrolase	GENE3D	G3DSA:3.40.50.1470		coord: 47..220 score: 1.3
IPR001328	Peptidyl-tRNA hydrolase	HAMAP	MF_00083	Pept_tRNA_hydro_bact	coord: 53..252 score: 28
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 233..269 score: 2.3E-168coord: 32..211 score: 2.3E
IPR001328	Peptidyl-tRNA hydrolase	PFAM	PF01195	Pept_tRNA_hydro	coord: 55..235 score: 1.1
IPR001328	Peptidyl-tRNA hydrolase	TIGRFAMs	TIGR00447	TIGR00447	coord: 55..211 score: 1.0
IPR001328	Peptidyl-tRNA hydrolase	unknown	SSF53178	Peptidyl-tRNA hydrolase-like	coord: 52..226 score: 4.71
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01195	PEPT_TRNA_HYDROL_1	coord: 66..79 scor
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01196	PEPT_TRNA_HYDROL_2	coord: 159..169 scor
None	No IPR available	PANTHER	PTHR17224:SF3	CHLOROPLASTIC GROUP IIB INTRON SPLICING FACILITATOR CRS2-A, CHLOROPLASTIC-RELATED	coord: 233..269 score: 2.3E-168coord: 32..211 score: 2.3E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G004130	Csa3G257600	Cucumber (Chinese Long) v2	culsiB212
Lsi05G004130	CSPI03G20250	Wild cucumber (PI 183967)	cpilsiB210
Lsi05G004130	Cucsa.031770	Cucumber (Gy14) v1	cgylsiB004
Lsi05G004130	CmaCh01G004030	Cucurbita maxima (Rimu)	cmalsiB440
Lsi05G004130	CmoCh01G004280	Cucurbita moschata (Rifu)	cmolsiB430
Lsi05G004130	Cla007137	Watermelon (97103) v1	lsiwmB369
Lsi05G004130	MELO3C023748	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G004130	ClCG05G012320	Watermelon (Charleston Gray)	lsiwcgB339
Lsi05G004130	MELO3C023748.2	Melon (DHL92) v3.6.1	lsimedB399
Lsi05G004130	Cla97C05G094130	Watermelon (97103) v2	lsiwmbB338
Lsi05G004130	Bhi01G001428	Wax gourd	lsiwgoB447
Lsi05G004130	Cp4.1LG02g13720	Cucurbita pepo (Zucchini)	cpelsiB470
Lsi05G004130	CsGy3G020030	Cucumber (Gy14) v2	cgyblsiB199
Lsi05G004130	Carg07497	Silver-seed gourd	carlsiB700
Lsi05G004130	CsaV3_3G020220	Cucumber (Chinese Long) v3	cuclsiB231

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G004130	Silver-seed gourd	carlsiB435
Lsi05G004130	Watermelon (97103) v2	lsiwmbB329
Lsi05G004130	Cucurbita maxima (Rimu)	cmalsiB249
Lsi05G004130	Cucurbita moschata (Rifu)	cmolsiB237
Lsi05G004130	Cucurbita pepo (Zucchini)	cpelsiB511
Lsi05G004130	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G004130	Watermelon (97103) v1	lsiwmB363