Lsi05G003860 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi05G003860
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionRegulator of Vps4 activity in the MVB pathway protein, putative
Locationchr05 : 4893281 .. 4895031 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCATCTTCCCTATAAAACATAACCAAAAAGTACCTCATTCAAGAGCTTCTCACAGACAAACAAGCAGCAAAAACAATTAAGGTTTTTCCATCATGGGAAGGAAACTGGATGCTCTACTGGGAAGGAACAGATATTTGAAGCCTTCCAAGTTTAAGACCCTTGCTAATATGGCCATTTCTAGGACTTCCATTCTAAAAAACCAGCACCGTGCTCGCAGTTCCCTTGCCCGTGCCGACGTTCTTCAGCTCTTGAACCTCAGTTACCAACATCGTGCTCAGCTTAGAGTAATTTGTTTCTCTCTCTGTCTCTCTAAACTCTACAGTTCAGCCCCTTATGTTTTCTTTTTCTTTTTTTGAATGCTGAGCTTGGATGTGAATGTGAATGAACAGGTTGAGATTGTGATCAAGGAGAATAACATGTTGGATGCACTTGGAATGATAGAGGATTACTGTTGCCTCCTCATAGAAAAAGTGGCTCTTCTTCAGAATAACAAGTAGGTGCCACCTTTCTTCAGTAGAATTTCAGTGTCATTTTTGTCAACTTCTTTGAATGCAAAGCCTTTTGAAAGTTTTGACACCTTTTCTGATTCTCTTCCGGCTTAAATGCAGGGAATGCCCTGGTGAAGTTAAGGAGGCAATATCGAGTCTGATCTTTGCAGCTTCAAGGTGTGGAGAGTTTCCAGAACTGCAGGAAATTCGTCGGATTGTCGAGTTGAAGTTTGGTACAGAGTTTGCAAGCAGTGCCGTTGATTTACGCAATAACTGTGGAGTTAGTCCCAAGGTGAGTTTGAAATCAACTGCAAGAAAAGAATTAGTTGCCTGCATATATCATCTTCCACTTAATAACCACAACTCCCATGTTACAGATGATCCAAAAGTTTTCAACAAAACAGCCCAACGCTGAAACTAAACTGAAAGTGCTAAAGGAAATTGCTTCGGAGAATGGCATTACTCTACATTTAGAGGAAGAACCAGCAATAATCATCAAGGTGAGCAAATTTCAAGCAGCAATGTATAGCAAATGAGTGTTTCTACTACTTAAGTTAACTCTTTAATAAAAATCCATCTGTTAACAGCAGGAAAAGCTAGACCAAAAGTTGGAAGCCAAGAAAGCAGCAGATTTGGATAATCCTGAAGTCATAAACACCACAGATAATCTACATGAAGTTATTGCAAGTGACGAGTCAGCATCAGAATCAGTGAAAGCAAAGAAGTTTACAGATGTAGCTAGTGCCGCTCAAGAAGCCTTCCAATCAGCAGCTTATGCAGCATTAGCAGCTAAAGCTGCAATCGAGCTTTCGATGTCTGAATCACAGGATATTGATTCAGATCATGAAGATGGTTCTCATCTTCAAATAAATCAAAAAAGCTCAGAATTAGATAGTTCTCATGAGCCTGAGCTTAATACAAAAGAAGCTAGCATGCCTTCCAGTAAGATTGACTATTCAGATGACAGATTAAGTTTCGAAAATATTTGTCCTGCTGAACTTGAAAGCTGCAGTTCAGAATATGAAGATGCAGTCATGGCAGAAAGCAACCAAGAAGAATTATCAAAAGAACCTGCCAAAGAAATTATGTCATCTGTTCTAAACTTGGATTCAGATATCAGCAAAATGAAGGAGCAATCATCAAAACCAAATTCAATCTTCTACCATGAGCCAGAAGGCGGCAAGTTAATCAGTAAAGAAGATGAAGTTGCCAGTAAAAATGAAATTTCAGAAGAGAATAACAGAGTTCACAAATCGAAGTAA

mRNA sequence

ATCATCTTCCCTATAAAACATAACCAAAAAGTACCTCATTCAAGAGCTTCTCACAGACAAACAAGCAGCAAAAACAATTAAGGTTTTTCCATCATGGGAAGGAAACTGGATGCTCTACTGGGAAGGAACAGATATTTGAAGCCTTCCAAGTTTAAGACCCTTGCTAATATGGCCATTTCTAGGACTTCCATTCTAAAAAACCAGCACCGTGCTCGCAGTTCCCTTGCCCGTGCCGACGTTCTTCAGCTCTTGAACCTCAGTTACCAACATCGTGCTCAGCTTAGAGTTGAGATTGTGATCAAGGAGAATAACATGTTGGATGCACTTGGAATGATAGAGGATTACTGTTGCCTCCTCATAGAAAAAGTGGCTCTTCTTCAGAATAACAAGGAATGCCCTGGTGAAGTTAAGGAGGCAATATCGAGTCTGATCTTTGCAGCTTCAAGGTGTGGAGAGTTTCCAGAACTGCAGGAAATTCGTCGGATTGTCGAGTTGAAGTTTGGTACAGAGTTTGCAAGCAGTGCCGTTGATTTACGCAATAACTGTGGAGTTAGTCCCAAGATGATCCAAAAGTTTTCAACAAAACAGCCCAACGCTGAAACTAAACTGAAAGTGCTAAAGGAAATTGCTTCGGAGAATGGCATTACTCTACATTTAGAGGAAGAACCAGCAATAATCATCAAGCAGGAAAAGCTAGACCAAAAGTTGGAAGCCAAGAAAGCAGCAGATTTGGATAATCCTGAAGTCATAAACACCACAGATAATCTACATGAAGTTATTGCAAGTGACGAGTCAGCATCAGAATCAGTGAAAGCAAAGAAGTTTACAGATGTAGCTAGTGCCGCTCAAGAAGCCTTCCAATCAGCAGCTTATGCAGCATTAGCAGCTAAAGCTGCAATCGAGCTTTCGATGTCTGAATCACAGGATATTGATTCAGATCATGAAGATGGTTCTCATCTTCAAATAAATCAAAAAAGCTCAGAATTAGATAGTTCTCATGAGCCTGAGCTTAATACAAAAGAAGCTAGCATGCCTTCCAGTAAGATTGACTATTCAGATGACAGATTAAGTTTCGAAAATATTTGTCCTGCTGAACTTGAAAGCTGCAGTTCAGAATATGAAGATGCAGTCATGGCAGAAAGCAACCAAGAAGAATTATCAAAAGAACCTGCCAAAGAAATTATGTCATCTGTTCTAAACTTGGATTCAGATATCAGCAAAATGAAGGAGCAATCATCAAAACCAAATTCAATCTTCTACCATGAGCCAGAAGGCGGCAAGTTAATCAGTAAAGAAGATGAAGTTGCCAGTAAAAATGAAATTTCAGAAGAGAATAACAGAGTTCACAAATCGAAGTAA

Coding sequence (CDS)

ATGGGAAGGAAACTGGATGCTCTACTGGGAAGGAACAGATATTTGAAGCCTTCCAAGTTTAAGACCCTTGCTAATATGGCCATTTCTAGGACTTCCATTCTAAAAAACCAGCACCGTGCTCGCAGTTCCCTTGCCCGTGCCGACGTTCTTCAGCTCTTGAACCTCAGTTACCAACATCGTGCTCAGCTTAGAGTTGAGATTGTGATCAAGGAGAATAACATGTTGGATGCACTTGGAATGATAGAGGATTACTGTTGCCTCCTCATAGAAAAAGTGGCTCTTCTTCAGAATAACAAGGAATGCCCTGGTGAAGTTAAGGAGGCAATATCGAGTCTGATCTTTGCAGCTTCAAGGTGTGGAGAGTTTCCAGAACTGCAGGAAATTCGTCGGATTGTCGAGTTGAAGTTTGGTACAGAGTTTGCAAGCAGTGCCGTTGATTTACGCAATAACTGTGGAGTTAGTCCCAAGATGATCCAAAAGTTTTCAACAAAACAGCCCAACGCTGAAACTAAACTGAAAGTGCTAAAGGAAATTGCTTCGGAGAATGGCATTACTCTACATTTAGAGGAAGAACCAGCAATAATCATCAAGCAGGAAAAGCTAGACCAAAAGTTGGAAGCCAAGAAAGCAGCAGATTTGGATAATCCTGAAGTCATAAACACCACAGATAATCTACATGAAGTTATTGCAAGTGACGAGTCAGCATCAGAATCAGTGAAAGCAAAGAAGTTTACAGATGTAGCTAGTGCCGCTCAAGAAGCCTTCCAATCAGCAGCTTATGCAGCATTAGCAGCTAAAGCTGCAATCGAGCTTTCGATGTCTGAATCACAGGATATTGATTCAGATCATGAAGATGGTTCTCATCTTCAAATAAATCAAAAAAGCTCAGAATTAGATAGTTCTCATGAGCCTGAGCTTAATACAAAAGAAGCTAGCATGCCTTCCAGTAAGATTGACTATTCAGATGACAGATTAAGTTTCGAAAATATTTGTCCTGCTGAACTTGAAAGCTGCAGTTCAGAATATGAAGATGCAGTCATGGCAGAAAGCAACCAAGAAGAATTATCAAAAGAACCTGCCAAAGAAATTATGTCATCTGTTCTAAACTTGGATTCAGATATCAGCAAAATGAAGGAGCAATCATCAAAACCAAATTCAATCTTCTACCATGAGCCAGAAGGCGGCAAGTTAATCAGTAAAGAAGATGAAGTTGCCAGTAAAAATGAAATTTCAGAAGAGAATAACAGAGTTCACAAATCGAAGTAA

Protein sequence

MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCGEFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLHLEEEPAIIIKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDESASESVKAKKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQINQKSSELDSSHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMAESNQEELSKEPAKEIMSSVLNLDSDISKMKEQSSKPNSIFYHEPEGGKLISKEDEVASKNEISEENNRVHKSK
Homology
[Close]
BLAST of Lsi05G003860 vs. Swiss-Prot
Match: IST1L_DICDI (IST1-like protein OS=Dictyostelium discoideum GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 3.2e-13
Identity = 48/163 (29.45%), Postives = 90/163 (55.21%), Query Frame = 1 

Query: 19  KFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKENNMLDAL 78
           K K    +A+SR  ILKN+        + +V +LL    +  A++RVE +I++  +++  
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 79  GMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCGEFPELQEIRRIVELKFGT 138
            +IE  C LL  ++ L+    E P E+KE+I +L++++ R  + PEL++I+  ++ K+G 
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 139 EFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASE 182
              + A +   +  V+PK++ K S   P+     + L EIA +
Sbjct: 132 GLENEA-NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEK 172

[Close]
BLAST of Lsi05G003860 vs. Swiss-Prot
Match: IST1_BOVIN (IST1 homolog OS=Bos taurus GN=IST1 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 9.5e-10
Identity = 46/172 (26.74%), Postives = 90/172 (52.33%), Query Frame = 1 

Query: 15  LKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKENNM 74
           +K  + +    + I+R  +L+ +    +  AR ++   L      RA++RVE +I+E+ +
Sbjct: 6   IKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYL 65

Query: 75  LDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRC-GEFPELQEIRRIVE 134
           ++A+ ++E YC LL+ +  L+Q+ KE    + E++S+LI+AA R   E  EL+ +   + 
Sbjct: 66  VEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLC 125

Query: 135 LKFGTEFASSAVDLRNNCG-VSPKMIQKFSTKQPNAETKLKVLKEIASENGI 185
            K+  E+    +   N  G V+ +++ K S + P      + L EIA    +
Sbjct: 126 AKYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

[Close]
BLAST of Lsi05G003860 vs. Swiss-Prot
Match: IST1_RAT (IST1 homolog OS=Rattus norvegicus GN=Ist1 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.6e-09
Identity = 46/171 (26.90%), Postives = 89/171 (52.05%), Query Frame = 1 

Query: 16  KPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKENNML 75
           K  + +    + I+R  +L+ +    +  AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRC-GEFPELQEIRRIVEL 135
           +A+ ++E YC LL+ +  L+Q+ KE    + E++S+LI+AA R   E  EL+ +   +  
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGTEFASSAVDLRNNCG-VSPKMIQKFSTKQPNAETKLKVLKEIASENGI 185
           K+  E+    +   N  G V+ +++ K S + P      + L EIA    +
Sbjct: 127 KYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

[Close]
BLAST of Lsi05G003860 vs. Swiss-Prot
Match: IST1_MOUSE (IST1 homolog OS=Mus musculus GN=Ist1 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.6e-09
Identity = 46/171 (26.90%), Postives = 89/171 (52.05%), Query Frame = 1 

Query: 16  KPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKENNML 75
           K  + +    + I+R  +L+ +    +  AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRC-GEFPELQEIRRIVEL 135
           +A+ ++E YC LL+ +  L+Q+ KE    + E++S+LI+AA R   E  EL+ +   +  
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGTEFASSAVDLRNNCG-VSPKMIQKFSTKQPNAETKLKVLKEIASENGI 185
           K+  E+    +   N  G V+ +++ K S + P      + L EIA    +
Sbjct: 127 KYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

[Close]
BLAST of Lsi05G003860 vs. Swiss-Prot
Match: IST1_HUMAN (IST1 homolog OS=Homo sapiens GN=IST1 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.6e-09
Identity = 46/171 (26.90%), Postives = 89/171 (52.05%), Query Frame = 1 

Query: 16  KPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKENNML 75
           K  + +    + I+R  +L+ +    +  AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRC-GEFPELQEIRRIVEL 135
           +A+ ++E YC LL+ +  L+Q+ KE    + E++S+LI+AA R   E  EL+ +   +  
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGTEFASSAVDLRNNCG-VSPKMIQKFSTKQPNAETKLKVLKEIASENGI 185
           K+  E+    +   N  G V+ +++ K S + P      + L EIA    +
Sbjct: 127 KYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

[Close]
BLAST of Lsi05G003860 vs. TrEMBL
Match: A0A0A0L6Y2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G271380 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 8.8e-172
Identity = 341/421 (81.00%), Postives = 369/421 (87.65%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLK SKFKTLANMAISRTSILKN HRAR SLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           AQLRVEIVIKENNMLDALGMIEDYCCLL+EKVALLQ NKECPGEVKEAISSLIFAASRCG
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRI ELKFG EFASSA+DLRNNCGVS KMIQKFSTKQP+AETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIIIKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDESASESVK 240
           ENGI LHLE+E  IIIK  K +QKLE KK ADL NPEV NTTD+LHEVI S++ ASE VK
Sbjct: 181 ENGIPLHLEQESPIIIKA-KQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVK 240

Query: 241 AKKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQINQKSSELDS 300
           +KKF DVASA +E FQSAAYAALAAKAAI LS S+SQDIDSDHEDGSHLQ N K S+L+S
Sbjct: 241 SKKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNS 300

Query: 301 SHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMAESNQEELSKEPA 360
           S+EP+LNTKE  + S KIDYSDDR SFE ICP ELESCSS+YEDA M E+NQEEL KEPA
Sbjct: 301 SYEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPA 360

Query: 361 KEIMSSVLNLDSDISKMKEQSSKPNSIFYHEPEGGKLISKEDEVASKNEISEENNRVHKS 420
           KE  S  L  DSD++ MKEQSSKPNSIFY+E +G K+ + ++EVAS  EISEE+ RVH+S
Sbjct: 361 KESTSFALK-DSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHES 419

Query: 421 K 422
           K
Sbjct: 421 K 419

[Close]
BLAST of Lsi05G003860 vs. TrEMBL
Match: A0A061EIJ4_THECC (Regulator of Vps4 activity in the MVB pathway protein, putative OS=Theobroma cacao GN=TCM_019471 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.4e-84
Identity = 219/437 (50.11%), Postives = 282/437 (64.53%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRN   K SKFKTLA +AISR +ILKNQH+ R S AR+DV+ LLNL +Q R
Sbjct: 1   MGRKLDALLGRN--FKASKFKTLAKLAISRIAILKNQHQVRFSHARSDVIDLLNLGHQER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           A LRVE VIKE N++DAL ++E YC LLIE+V L+Q NKECP E+KEA SSL+FA+SRCG
Sbjct: 61  ALLRVEHVIKEQNLVDALTIMESYCQLLIERVMLIQKNKECPDELKEATSSLMFASSRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPEL  IRR+V   FG EF + AV+LRNNC V PK++QK ST+QP+ E KLKVLKEIAS
Sbjct: 121 EFPELLHIRRVVSSTFGKEFVARAVELRNNCSVHPKIVQKLSTRQPSLECKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIIIKQEKLD-----QKLEAKKAADLDNPEVINTTDNLHEVIASDESA 240
           E GI LHLE +  +++ +EK+D      +  A K+A+LD+PE   T   L   +  DE  
Sbjct: 181 ERGIALHLEVDSPVVV-EEKMDVHEKQNESTANKSANLDDPEYKYTAIELPREVNLDEKL 240

Query: 241 SESVKA-KKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQINQK 300
           SESVKA KK+ DVA+AAQEAF+SAAYAA AA+AA+ELS +ESQD D D E GS  +    
Sbjct: 241 SESVKARKKYRDVAAAAQEAFESAAYAAAAARAAVELSRTESQDFDPDDESGSSHKKGGP 300

Query: 301 S------SELDSSHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMA 360
           S       + D+S  PE    EA   +  I++ ++ L FE I P +  S  SE ED    
Sbjct: 301 SLKKGAFYDSDASATPEFQGYEA---ADGIEHPNNELGFEKIHPIDNYSSESEGEDMTKT 360

Query: 361 ----ESNQ-EELSKEPAKEIMSSVLNLDSDISKMKEQSSKPNSIFYHEPEGGKLISKEDE 420
               E N+ EE+ +    E   S  + DSD+    E+    + +  +E  G +L    D 
Sbjct: 361 NNGLELNEIEEIEQRVWIERALSTSSSDSDVDISTERKLSSDQLRQNEQLGDELAI--DL 420

[Close]
BLAST of Lsi05G003860 vs. TrEMBL
Match: A0A067F674_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g010606mg PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.3e-71
Identity = 187/411 (45.50%), Postives = 267/411 (64.96%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           M RKLD L GR+     SKFKT+  +AISR +IL+NQH+AR   AR+DV +LLNL +  R
Sbjct: 1   MSRKLDRLFGRS--FNTSKFKTVTKLAISRAAILRNQHQARYYYARSDVTELLNLGHHER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           A  RVE +IKE NMLDALGMIEDYC LLIE+V L+Q NKECP E+KEAIS LIFA+SRCG
Sbjct: 61  ALTRVEYMIKELNMLDALGMIEDYCHLLIERVLLIQKNKECPDELKEAISGLIFASSRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           +FPELQ+IR I + ++G EF + AV+LRN CGV+PK++QK S+++ + E++LKVLKEI++
Sbjct: 121 DFPELQKIREIFKSRYGAEFDARAVELRNYCGVTPKIVQKLSSRRCSLESRLKVLKEIST 180

Query: 181 ENGIT-LHLEEEPAIIIKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDESASESV 240
           E GI  L+LEE+  + +K EK D  +E K+   ++     +   N+ E +  DE  SESV
Sbjct: 181 EMGIALLNLEEDTRVPVK-EKSD--IEQKQPKPMEPATFNDDAHNVPEELNRDEKLSESV 240

Query: 241 KAKK-FTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQINQKSSEL 300
           K +K + DVA+AAQ+AF+SAAYAA AA+AA +LS SES+D +SD + G +    + +S  
Sbjct: 241 KRRKYYRDVAAAAQDAFESAAYAAEAARAAFDLSRSESRDNNSD-DFGGYSHGTEIASNS 300

Query: 301 DSSHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMAESNQ------ 360
           D S   +L + + ++ + + + S  R+ F+     +++S SSE  D  + E  +      
Sbjct: 301 DMSMTSDLKSDKDAVINEEDEDSKSRMGFDQF--YDVDSFSSESGDEDVTEKTREIHFKE 360

Query: 361 -EELSKEPAKEIMSSVLNLDSDISKMKEQSSKPNSIFYHEPEGGKLISKED 403
            EE  +   +E + S  + DSD   +   + K     Y   EGG  ++ +D
Sbjct: 361 LEESERTAVRERILSASSSDSD-GNILSMTKKLAGAHYRNQEGGIAVASDD 402

[Close]
BLAST of Lsi05G003860 vs. TrEMBL
Match: B9RSL0_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1725700 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.8e-69
Identity = 178/359 (49.58%), Postives = 234/359 (65.18%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MG  L   LGR    K SKFKT+A +AISR +ILKNQ + R S A++DV++LLN  +Q R
Sbjct: 1   MGALLAVFLGRK--FKASKFKTIAKLAISRVTILKNQKQVRYSHAKSDVVELLNCGHQER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           A LRVE +IKE N++DA  MIEDYC LLI+++ LL+  KEC  EVKEAIS+LIFA+SRCG
Sbjct: 61  ALLRVEHMIKEQNLVDAFIMIEDYCYLLIDRITLLKKEKECHDEVKEAISTLIFASSRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIR I E KFG EFA+ A++LRNNCGV+PK+IQK S ++P+ E ++KVLKE AS
Sbjct: 121 EFPELQEIRGIFESKFGKEFAARAIELRNNCGVNPKIIQKLSVRRPSLENRMKVLKETAS 180

Query: 181 ENGITLHLEEEPAIIIKQEKLDQKL-----EAKKAADLDNPEVINTTDNLHEVIASDESA 240
           E GI LHLEE+   ++ +EKLD K      + ++  ++D  E  +    L EV+   E  
Sbjct: 181 EKGIVLHLEEDTP-VVAEEKLDDKKKKMIPQQQQQEEIDETEPQDKPLVLIEVLNPVEKL 240

Query: 241 SESVKAK-KFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQINQK 300
           S+S KA+ K+ DV +AA EAF+SAAYAA AA+AA+ELS  + Q+ D D  +G        
Sbjct: 241 SKSTKARNKYRDVTAAALEAFESAAYAASAARAAVELSRPKCQESDRDDHNG-------- 300

Query: 301 SSELDSSHEPELNTK--EASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMAESN 352
                 SH    N K   A     K++ S+D   F+ I   +  +  SE ED  MAE+N
Sbjct: 301 -----FSHPGPTNCKLVIAYEDPEKMENSNDTSVFKKIHLVDYSNSESESED--MAENN 341

[Close]
BLAST of Lsi05G003860 vs. TrEMBL
Match: K4BXC9_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.4e-68
Identity = 187/414 (45.17%), Postives = 255/414 (61.59%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRN   K SKFK  AN+AISR ++LKNQ +AR S+AR+DV+QLLNL Y  R
Sbjct: 1   MGRKLDALLGRN--FKTSKFKATANLAISRVTVLKNQRQARCSIARSDVVQLLNLGYHER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           A LRVE VIKE NMLD   M+E YC L IE++ L+Q  K CP E+KEAISSLIFAASRCG
Sbjct: 61  ALLRVEQVIKEQNMLDVFDMVEGYCLLTIERINLIQQEKICPEELKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQE+R I   +FG EFA+ AV+LRNNCGV+ KMIQK ST+ P+ E + KVLKEIA+
Sbjct: 121 EFPELQELRGIFTSRFGKEFAARAVELRNNCGVNTKMIQKLSTRMPSLEQRTKVLKEIAA 180

Query: 181 ENGITLHLEEEPAIIIKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDESASESVK 240
           EN I L +EE    I+ +   ++++ AK+  + ++ E +   D  HE             
Sbjct: 181 ENNIVLKIEE----IMLENTEEKEVTAKRKDEPEDHESVLPQDVDHEA------------ 240

Query: 241 AKKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQINQKSSELDS 300
            +KF DV +AAQ AF+SAAYAA AA+AA+ELS SES+D D D +  S    +   S  D 
Sbjct: 241 RRKFKDVEAAAQAAFESAAYAAAAARAAVELSRSESRDTD-DPKSPSQKPRDVSDSREDL 300

Query: 301 SHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMAESNQEELSKEPA 360
             E     ++ +    KI+ S +   F     +E++  S   E   + E  Q++  K+  
Sbjct: 301 KSEFHAGEEKNNEGVEKIETSQN-YGFH----SEVDQFSDGDE---VEEVEQKKFEKQSK 360

Query: 361 KEIMSSVLNLDSDISKMKEQSSKPNSIFYHEPEGGKLISKEDEVAS----KNEI 411
           + +  S  ++ +D   + E  S  + + Y E +  + + +E+ + S    KNE+
Sbjct: 361 RSVSVSSSDISADDILVDEVKSPRHGVIYDEID--EEVGEENRIPSLKHPKNEV 385

[Close]
BLAST of Lsi05G003860 vs. TAIR10
Match: AT1G13340.1 (AT1G13340.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 198.4 bits (503), Expect = 9.0e-51
Identity = 149/394 (37.82%), Postives = 223/394 (56.60%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MG+KLDALLGR+   K +KFK+L  +A++R SILKNQ +AR S A +DV +LL L     
Sbjct: 1   MGKKLDALLGRS--FKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEH 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           A  RV+ V+K+ N LD L  I  Y  L ++++ L ++N++CP E+ EA+S L+FAASR G
Sbjct: 61  AYHRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIR ++  +FG + A+ +++LR+NCGV PK+IQK ST+ P  E ++K LKEIA+
Sbjct: 121 EFPELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAA 180

Query: 181 ENGITLHLEEEPAIIIKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDESASESVK 240
           EN I L L++          +    +  K         + + D   E        S+SVK
Sbjct: 181 ENNIVLKLDQASTSTEGTTNMQGTSDVSKTK-------LTSKDGRGE----GYGLSDSVK 240

Query: 241 --AKKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDS----DHEDGSHLQINQK 300
              KK+ DVA AAQ AF+SAA+AA AA+AA+ELS    +  DS      E+  H   N++
Sbjct: 241 RGKKKYKDVADAAQAAFESAAHAAEAAQAAVELSQFSPRGHDSPGNIGGENSFHGSENKE 300

Query: 301 SSELDSSHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSE-----YEDAVMAE 360
           S +    ++     +   M  SK   SD     E+I    + S   +      +D ++ +
Sbjct: 301 SEQEQEGNDDSSEGEGDVMSESKRSMSDS----EDIIDVPVMSFREDPVKLLEKDTIIYD 360

Query: 361 SNQEELSKEPAKEIMSSVLNLDSDISKMKEQSSK 384
           S +E    +P+ E ++        ISK K++ ++
Sbjct: 361 SEEE---TQPSVETIT--------ISKSKDEQNR 366

[Close]
BLAST of Lsi05G003860 vs. TAIR10
Match: AT4G35730.1 (AT4G35730.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 133.7 bits (335), Expect = 2.7e-31
Identity = 105/316 (33.23%), Postives = 159/316 (50.32%), Query Frame = 1 

Query: 13  RYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKEN 72
           R    SK KT A MA++R  +++N+        R D+  LL       A++RVE VI+E 
Sbjct: 24  RGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQ 83

Query: 73  NMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCGEFPELQEIRRIV 132
           N+  A  +IE +C L++ ++ ++   K+CP ++KE I+SLIFAA RC E PEL ++R I 
Sbjct: 84  NIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIF 143

Query: 133 ELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLHLEEEP 192
             K+G +F S+A DLR +CGV+  +I K S + P  E KLK++KEIA E  +     E  
Sbjct: 144 AKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETE 203

Query: 193 AIIIK--QEKLDQKLEAKKAADLD-NPEVINTTDNLHEVIASDESASESVKAKKFTDVAS 252
             ++K  +E +D   +   A+ L  N   IN  + +    A   S S       + D  S
Sbjct: 204 QELLKPQEESIDGPRKFVSASSLPVNRAAIN--EPIDPTKAVPRSTSSMSINTHYHDTES 263

Query: 253 AAQEAFQSAAYAALAAKAAIEL-----SMSESQDIDSDHEDGSHLQINQKSSELDSSHEP 312
           AA+ A + A  A  AA+ A  L     S ++   + SDH        +QK S+    H  
Sbjct: 264 AAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDH------STHQKDSQYMDHHHH 323

Query: 313 ELNTKEASMPSSKIDY 321
              ++  S  S    Y
Sbjct: 324 HPGSRRQSRDSETSSY 331

[Close]
BLAST of Lsi05G003860 vs. TAIR10
Match: AT4G29440.1 (AT4G29440.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 120.2 bits (300), Expect = 3.1e-27
Identity = 61/178 (34.27%), Postives = 107/178 (60.11%), Query Frame = 1 

Query: 12  NRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKE 71
           +R  KP+K K    MA SR  ILKN+   +    R ++  LL       A++RVE V++E
Sbjct: 6   HRSFKPAKCKIALQMAASRLKILKNKKDTQIKQLRRELAHLLESGQTQTAKIRVEHVVRE 65

Query: 72  NNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCGEFPELQEIRRI 131
              + A  ++  YC LL+ ++ ++ + K CP ++KEA++S+++A+ R  +  EL +I + 
Sbjct: 66  EKTVAAYELVGIYCELLVARLGVIDSQKTCPNDLKEAVASVLYASQRLTDVGELSDIVKH 125

Query: 132 VELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLHLE 190
              K+G +F S+A+ L+ + GVS  +++K S K P+  TK+K+L EIA+++ +T   E
Sbjct: 126 FSAKYGKDFVSAAIGLQPDSGVSRLLVEKLSVKAPDGPTKIKILTEIATQHNVTWEAE 183

[Close]
BLAST of Lsi05G003860 vs. TAIR10
Match: AT1G25420.1 (AT1G25420.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 116.3 bits (290), Expect = 4.5e-26
Identity = 81/266 (30.45%), Postives = 140/266 (52.63%), Query Frame = 1 

Query: 12  NRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKE 71
           NR +  +K KT  N+AI+R  +L+N+   +    + ++   L    +  A++RVE VI+E
Sbjct: 9   NRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVIRE 68

Query: 72  NNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCGEFPELQEIRRI 131
            N+  A  ++E +C  ++ +V +L++ KECP E++EAI+S+IFAA RC E P+L +I+ +
Sbjct: 69  MNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNL 128

Query: 132 VELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLHLEEE 191
              K+G EF   A +LR + GV+  +I+K S   P+   +LK+LKEIA E  +       
Sbjct: 129 FGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSAT 188

Query: 192 PAIIIKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDESASESVKAKKFTDVASAA 251
            A  +K  +            L   + I+  D + E   S +   +S  +++   + + A
Sbjct: 189 EAEFMKSHE----------DLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEA 248

Query: 252 QEAFQSAAYAALAAKAAIELSMSESQ 278
            + FQ      L A+  +  SM  S+
Sbjct: 249 TQRFQK-----LQAQNPVSKSMPSSK 259

[Close]
BLAST of Lsi05G003860 vs. TAIR10
Match: AT2G19710.1 (AT2G19710.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 105.1 bits (261), Expect = 1.0e-22
Identity = 107/391 (27.37%), Postives = 195/391 (49.87%), Query Frame = 1 

Query: 13  RYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHRAQLRVEIVIKEN 72
           R  KP+K KT   MA SR  ILKN+   +    R ++ QLL       A++RVE V++E 
Sbjct: 7   RGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREE 66

Query: 73  NMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCGEFPELQEIRRIV 132
             + A  +I  YC LL+ ++ ++++ K CP ++KEA++S++FA+ R  + PEL EI +  
Sbjct: 67  KTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQF 126

Query: 133 ELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIASENGITLH----L 192
             K+G +F++SAV+LR + GVS  +++K S K P+  TK+K+L  IA E+ +       +
Sbjct: 127 TTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWEAQSFV 186

Query: 193 EEEPAIIIKQEKLDQKLEAKKAADLDNPEVINTT----DNLHEVIASDESASESVKAKKF 252
           E +P      E L+     + A+ ++    IN+      N+H    +  +A      +  
Sbjct: 187 ESDPK---DTELLNGANSFQPASSMNMDSSINSNKEQPPNIH--APATVNAHHGSSERHH 246

Query: 253 TDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQIN----QKSSELDS 312
           +   S A     S+    + +  A +   S+++   S  ++G     N      SS    
Sbjct: 247 SPENSYANGGRSSSRSNNVTSGKADDYYHSKARPSRSRPDEGECRNPNHGYENSSSRNKQ 306

Query: 313 SHEPE-LNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMAESNQEELSKEP 372
             EPE +++ +A+  +++   + +R SF     AEL              SN+E ++++ 
Sbjct: 307 KWEPEFVDSTDAARAAAE---AAERASFAARAAAEL--------------SNKERMTRQD 366

Query: 373 AKE--IMSSVLNLDSDISKMKEQSSKPNSIF 389
           + +  I S+ +NL ++ S  +++S+     F
Sbjct: 367 STQSHISSASVNLRNEPSHRRDRSNAQRESF 375

[Close]
BLAST of Lsi05G003860 vs. NCBI nr
Match: gi|659123287|ref|XP_008461584.1| (PREDICTED: uncharacterized protein LOC103500154 [Cucumis melo])

HSP 1 Score: 616.3 bits (1588), Expect = 3.9e-173
Identity = 345/421 (81.95%), Postives = 369/421 (87.65%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRAR SLARADVLQLLNL YQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARCSLARADVLQLLNLGYQHR 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           AQLRVEIVIKENNMLDALGMIEDYCCLL+EKVAL Q NKECPGEVKEAISSLIFAASRCG
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALFQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRI ELKFGTEFASSA+DLRNNCGVSPKMIQKFSTKQP AETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGTEFASSAIDLRNNCGVSPKMIQKFSTKQPTAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIIIKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDESASESVK 240
           ENGI L+LEEEP II+K EKL+QKLE KK ADL NPEV NTTD+LHEVI S++ ASE VK
Sbjct: 181 ENGIPLNLEEEPPIIVK-EKLNQKLEVKKEADLYNPEVTNTTDDLHEVITSEKLASELVK 240

Query: 241 AKKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQINQKSSELDS 300
           AKKF DVASAA+EAFQSAA        AI    SESQDIDSDHEDGSHLQ N KSSEL+S
Sbjct: 241 AKKFKDVASAAEEAFQSAA-------KAINFLKSESQDIDSDHEDGSHLQKNGKSSELNS 300

Query: 301 SHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMAESNQEELSKEPA 360
           S+E +LNTKEAS+PSSKIDYSDDR SFE ICP  LESCSS+YEDA M E+NQEEL KEPA
Sbjct: 301 SYESKLNTKEASIPSSKIDYSDDRFSFEKICPVGLESCSSQYEDADMEETNQEELPKEPA 360

Query: 361 KEIMSSVLNLDSDISKMKEQSSKPNSIFYHEPEGGKLISKEDEVASKNEISEENNRVHKS 420
            E  SSVLNL SDI+  KEQSS+PNS FY+E +G K+ISK++EVAS  EISEEN +VH+ 
Sbjct: 361 IESTSSVLNLCSDINNTKEQSSEPNSTFYNESDGDKIISKDNEVASGKEISEENIKVHEP 413

Query: 421 K 422
           K
Sbjct: 421 K 413

[Close]
BLAST of Lsi05G003860 vs. NCBI nr
Match: gi|778680668|ref|XP_011651369.1| (PREDICTED: uncharacterized protein LOC105434874 isoform X1 [Cucumis sativus])

HSP 1 Score: 611.3 bits (1575), Expect = 1.3e-171
Identity = 341/421 (81.00%), Postives = 369/421 (87.65%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLK SKFKTLANMAISRTSILKN HRAR SLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           AQLRVEIVIKENNMLDALGMIEDYCCLL+EKVALLQ NKECPGEVKEAISSLIFAASRCG
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRI ELKFG EFASSA+DLRNNCGVS KMIQKFSTKQP+AETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIIIKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDESASESVK 240
           ENGI LHLE+E  IIIK  K +QKLE KK ADL NPEV NTTD+LHEVI S++ ASE VK
Sbjct: 181 ENGIPLHLEQESPIIIKA-KQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVK 240

Query: 241 AKKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQINQKSSELDS 300
           +KKF DVASA +E FQSAAYAALAAKAAI LS S+SQDIDSDHEDGSHLQ N K S+L+S
Sbjct: 241 SKKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNS 300

Query: 301 SHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMAESNQEELSKEPA 360
           S+EP+LNTKE  + S KIDYSDDR SFE ICP ELESCSS+YEDA M E+NQEEL KEPA
Sbjct: 301 SYEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPA 360

Query: 361 KEIMSSVLNLDSDISKMKEQSSKPNSIFYHEPEGGKLISKEDEVASKNEISEENNRVHKS 420
           KE  S  L  DSD++ MKEQSSKPNSIFY+E +G K+ + ++EVAS  EISEE+ RVH+S
Sbjct: 361 KESTSFALK-DSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHES 419

Query: 421 K 422
           K
Sbjct: 421 K 419

[Close]
BLAST of Lsi05G003860 vs. NCBI nr
Match: gi|778680671|ref|XP_011651370.1| (PREDICTED: uncharacterized protein LOC105434874 isoform X2 [Cucumis sativus])

HSP 1 Score: 461.8 bits (1187), Expect = 1.2e-126
Identity = 253/291 (86.94%), Postives = 265/291 (91.07%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLK SKFKTLANMAISRTSILKN HRAR SLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           AQLRVEIVIKENNMLDALGMIEDYCCLL+EKVALLQ NKECPGEVKEAISSLIFAASRCG
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPELQEIRRI ELKFG EFASSA+DLRNNCGVS KMIQKFSTKQP+AETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIIIKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDESASESVK 240
           ENGI LHLE+E  IIIK  K +QKLE KK ADL NPEV NTTD+LHEVI S++ ASE VK
Sbjct: 181 ENGIPLHLEQESPIIIK-AKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVK 240

Query: 241 AKKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQI 292
           +KKF DVASA +E FQSAAYAALAAKAAI LS S+SQDIDSDHEDGSHL +
Sbjct: 241 SKKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLPV 290

[Close]
BLAST of Lsi05G003860 vs. NCBI nr
Match: gi|590652944|ref|XP_007033286.1| (Regulator of Vps4 activity in the MVB pathway protein, putative [Theobroma cacao])

HSP 1 Score: 320.9 bits (821), Expect = 3.4e-84
Identity = 219/437 (50.11%), Postives = 282/437 (64.53%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRN   K SKFKTLA +AISR +ILKNQH+ R S AR+DV+ LLNL +Q R
Sbjct: 1   MGRKLDALLGRN--FKASKFKTLAKLAISRIAILKNQHQVRFSHARSDVIDLLNLGHQER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           A LRVE VIKE N++DAL ++E YC LLIE+V L+Q NKECP E+KEA SSL+FA+SRCG
Sbjct: 61  ALLRVEHVIKEQNLVDALTIMESYCQLLIERVMLIQKNKECPDELKEATSSLMFASSRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
           EFPEL  IRR+V   FG EF + AV+LRNNC V PK++QK ST+QP+ E KLKVLKEIAS
Sbjct: 121 EFPELLHIRRVVSSTFGKEFVARAVELRNNCSVHPKIVQKLSTRQPSLECKLKVLKEIAS 180

Query: 181 ENGITLHLEEEPAIIIKQEKLD-----QKLEAKKAADLDNPEVINTTDNLHEVIASDESA 240
           E GI LHLE +  +++ +EK+D      +  A K+A+LD+PE   T   L   +  DE  
Sbjct: 181 ERGIALHLEVDSPVVV-EEKMDVHEKQNESTANKSANLDDPEYKYTAIELPREVNLDEKL 240

Query: 241 SESVKA-KKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGSHLQINQK 300
           SESVKA KK+ DVA+AAQEAF+SAAYAA AA+AA+ELS +ESQD D D E GS  +    
Sbjct: 241 SESVKARKKYRDVAAAAQEAFESAAYAAAAARAAVELSRTESQDFDPDDESGSSHKKGGP 300

Query: 301 S------SELDSSHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMA 360
           S       + D+S  PE    EA   +  I++ ++ L FE I P +  S  SE ED    
Sbjct: 301 SLKKGAFYDSDASATPEFQGYEA---ADGIEHPNNELGFEKIHPIDNYSSESEGEDMTKT 360

Query: 361 ----ESNQ-EELSKEPAKEIMSSVLNLDSDISKMKEQSSKPNSIFYHEPEGGKLISKEDE 420
               E N+ EE+ +    E   S  + DSD+    E+    + +  +E  G +L    D 
Sbjct: 361 NNGLELNEIEEIEQRVWIERALSTSSSDSDVDISTERKLSSDQLRQNEQLGDELAI--DL 420

[Close]
BLAST of Lsi05G003860 vs. NCBI nr
Match: gi|359488181|ref|XP_002280096.2| (PREDICTED: uncharacterized protein LOC100249716 [Vitis vinifera])

HSP 1 Score: 297.0 bits (759), Expect = 5.3e-77
Identity = 191/369 (51.76%), Postives = 255/369 (69.11%), Query Frame = 1 

Query: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARSSLARADVLQLLNLSYQHR 60
           MG+KLD LLGR+   K SK KTL N+AISR ++LKNQ + R S AR DV+QLLNL +Q  
Sbjct: 1   MGKKLDYLLGRS--FKISKLKTLVNLAISRLAVLKNQRQVRCSHARTDVIQLLNLGHQEP 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLIEKVALLQNNKECPGEVKEAISSLIFAASRCG 120
           A LRVE VIKE NMLD   MIE YC LLIE++ L QN KECP E+KEA+SSLIFA SRCG
Sbjct: 61  ALLRVEYVIKEQNMLDVFLMIEAYCHLLIERITLFQN-KECPDELKEAVSSLIFATSRCG 120

Query: 121 EFPELQEIRRIVELKFGTEFASSAVDLRNNCGVSPKMIQKFSTKQPNAETKLKVLKEIAS 180
            FPELQ+IR +   +FG EFA+ A +L+NNCGV+ KMIQ+ ST+Q + E++LKVL EIAS
Sbjct: 121 GFPELQQIREMFVSRFGKEFAARAAELQNNCGVNLKMIQRLSTRQASLESRLKVLTEIAS 180

Query: 181 ENGITLHLEEEPAII------IKQEKLDQKLEAKKAADLDNPEVINTTDNLHEVIASDES 240
           ENGITL LEE+ A+       + Q++  ++LE+ ++A+LDNPE+ +  ++  E+I   E+
Sbjct: 181 ENGITLSLEEDAALSTEEKQDVNQKQKRKQLESNESANLDNPELKDDNNDFPELINCGET 240

Query: 241 ASESVKAKKFTDVASAAQEAFQSAAYAALAAKAAIELSMSESQDIDSDHEDGS-HLQINQ 300
            SESVKA+K+ DVASAAQ+AF+SAAYAA AA+AA+ELS +ES D D D+  GS H +   
Sbjct: 241 LSESVKARKYRDVASAAQDAFKSAAYAAAAARAAVELSRAESWDQDPDYYSGSKHQRRIM 300

Query: 301 KSSELDSSHEPELNTKEASMPSSKIDYSDDRLSFENICPAELESCSSEYEDAVMAESNQE 360
            SS+  S+ + +++T         I+ S+D L F+ I P +  S  SE ++    E N +
Sbjct: 301 SSSDGSSTSKLQVDTNAF---LQDIEKSNDGLGFDMIHPTDNLSSESERDE---MEDNLD 360

Query: 361 ELSKEPAKE 363
               +  KE
Sbjct: 361 RSHLQEMKE 360

The following BLAST results are available for this feature:
BLAST of Lsi05G003860 vs. Swiss-Prot
Match NameE-valueIdentityDescription
IST1L_DICDI3.2e-1329.45IST1-like protein OS=Dictyostelium discoideum GN=DDB_G0289029 PE=3 SV=1[more]
IST1_BOVIN9.5e-1026.74IST1 homolog OS=Bos taurus GN=IST1 PE=2 SV=1[more]
IST1_RAT1.6e-0926.90IST1 homolog OS=Rattus norvegicus GN=Ist1 PE=2 SV=1[more]
IST1_MOUSE1.6e-0926.90IST1 homolog OS=Mus musculus GN=Ist1 PE=1 SV=1[more]
IST1_HUMAN1.6e-0926.90IST1 homolog OS=Homo sapiens GN=IST1 PE=1 SV=1[more]
BLAST of Lsi05G003860 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L6Y2_CUCSA8.8e-17281.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G271380 PE=4 SV=1[more]
A0A061EIJ4_THECC2.4e-8450.11Regulator of Vps4 activity in the MVB pathway protein, putative OS=Theobroma cac... [more]
A0A067F674_CITSI2.3e-7145.50Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g010606mg PE=4 SV=1[more]
B9RSL0_RICCO2.8e-6949.58Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1725700 PE=4 SV=1[more]
K4BXC9_SOLLC1.4e-6845.17Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1[more]
BLAST of Lsi05G003860 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G13340.19.0e-5137.82 Regulator of Vps4 activity in the MVB pathway protein[more]
AT4G35730.12.7e-3133.23 Regulator of Vps4 activity in the MVB pathway protein[more]
AT4G29440.13.1e-2734.27 Regulator of Vps4 activity in the MVB pathway protein[more]
AT1G25420.14.5e-2630.45 Regulator of Vps4 activity in the MVB pathway protein[more]
AT2G19710.11.0e-2227.37 Regulator of Vps4 activity in the MVB pathway protein[more]
BLAST of Lsi05G003860 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659123287|ref|XP_008461584.1|3.9e-17381.95PREDICTED: uncharacterized protein LOC103500154 [Cucumis melo][more]
gi|778680668|ref|XP_011651369.1|1.3e-17181.00PREDICTED: uncharacterized protein LOC105434874 isoform X1 [Cucumis sativus][more]
gi|778680671|ref|XP_011651370.1|1.2e-12686.94PREDICTED: uncharacterized protein LOC105434874 isoform X2 [Cucumis sativus][more]
gi|590652944|ref|XP_007033286.1|3.4e-8450.11Regulator of Vps4 activity in the MVB pathway protein, putative [Theobroma cacao... [more]
gi|359488181|ref|XP_002280096.2|5.3e-7751.76PREDICTED: uncharacterized protein LOC100249716 [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0015031protein transport
Vocabulary: INTERPRO
TermDefinition
IPR005061Ist1
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
biological_process GO:0006950 response to stress
biological_process GO:0045087 innate immune response
biological_process GO:0031348 negative regulation of defense response
biological_process GO:0006979 response to oxidative stress
biological_process GO:0009697 salicylic acid biosynthetic process
biological_process GO:0009863 salicylic acid mediated signaling pathway
biological_process GO:0006952 defense response
biological_process GO:0050789 regulation of biological process
biological_process GO:0044763 single-organism cellular process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G003860.1Lsi05G003860.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161UNCHARACTERIZED DUF292coord: 2..343
score: 3.6E
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 21..184
score: 4.9
NoneNo IPR availablePANTHERPTHR12161:SF23REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 2..343
score: 3.6E