Csa3G271380 (gene) Cucumber (Chinese Long) v2

NameCsa3G271380
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionIST1-like protein; contains IPR005061 (Domain of unknown function DUF292, eukaryotic)
LocationChr3 : 16451738 .. 16453863 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGGAAACTGGATGCTTTACTGGGAAGGAATAGATATTTGAAGGCTTCCAAGTTTAAGACCCTTGCTAATATGGCCATTTCTAGGACTTCCATCCTAAAAAACCACCACCGCGCCCGTTGCTCCCTAGCTCGTGCCGATGTTCTTCAACTGTTGAATCTTAGTTACCAACATCGTGCTCAGCTTAGAGTAAATTTTTTCTCTCTCTCTTTCAGAACTCTATAGTTCAGCCCCTTCTCATTTTTTTCTTTTTTTCCAACCGCTGAGCTTGGATGTGAACGTTAATGAACAGGTTGAGATTGTGATCAAGGAGAATAACATGTTGGATGCTCTTGGAATGATAGAGGATTACTGCTGCCTTCTCGTAGAAAAGGTGGCTCTTCTTCAGATGAACAAGTAGGTCACCTTTCTTTCCTAGAATTTCACTATGTCATTTTTGTCAACTTTGGATGCAGGCCTTTTAAAAGATTTGAAACCTTTTCTGATTCTCTTTCGGCTTAAATGCAGGGAATGCCCTGGCGAAGTTAAGGAGGCGATATCGAGTCTGATATTTGCAGCTTCAAGGTGCGGAGAGTTTCCAGAACTTCAAGAGATTCGTCGGATTTTCGAGTTGAAGTTTGGTGCGGAGTTTGCAAGCAGTGCCATTGATTTACGCAACAACTGTGGTGTTAGTACCAAGGTGAGTTTGATACTTACCAACTGCAAGAAACGAATTAGTTGTTCGAATATATAATCTCCACTTAATAACCACAGTTTCCATATTACAGATGATTCAAAAGTTTTCAACAAAACAGCCCAGTGCCGAAACTAAACTGAAAGTGCTAAAGGAAATTGCTTCAGAAAATGGCATTCCTCTCCATTTAGAGCAAGAATCACCAATAATCATCAAGGTGAGGAAATATCTTTTTTTTTTTTTTAATTATAATGAAAAGAAACATTTCATTCATATGATAGTGAAGAGCCCCAAACATCAAAAAAGGTGATTACATAAGGGAGCGCCAATTGCTAACTAAAAAATATAAGCTTGAATGGAAAGGTGCTTTATTTTAAACTAAGAAAAAGCGATTAGAAGAGGAAAAAGTGTCACCAAAGAGCCGTATGTTCCTCACCTTGATAGAAAATGAGTGCTTCTACTTCCTAAGTTAACTTTTTAGTAAAAAATTCATCTGTTAACTGCAGGCAAAGCAAAACCAAAAGTTGGAAATCAAGAAAGAAGCAGATTTGTATAATCCTGAAGTCACAAACACCACAGATGATCTACATGAAGTTATTACAAGTGAAAAGATGGCATCGGAATTAGTGAAATCAAAGAAGTTTAAAGATGTAGCTAGTGCCACTGAAGAACCCTTCCAATCGGCAGCTTATGCAGCATTAGCAGCTAAAGCTGCAATCAACCTTTCAAAGTCCAAATCACAGGATATTGATTCAGATCATGAAGATGGCTCTCATCTACAAAAGAATGGAAAAGGCTCAGATTTGAATAGTTCTTATGAACCTAAGCTTAATACAAAAGAAGAAAGAATATCTTCCTGTAAGATTGACTATTCAGACGACAGATTTAGTTTTGAAAAGATTTGTCCTGTTGAACTTGAAAGTTGCAGTTCTCAATATGAAGATGCTGACATGGAAGAAACCAATCAAGAAGAGTTACCAAAAGAACCTGCCAAAGAAAGCACATCCTTTGCTCTAAAAGATTCAGATGTTAACAATATGAAGGAGCAATCATCAAAACCAAATTCAATCTTCTACAACGAGTCAGACGGCGACAAGATAACCAATACAGATAATGAAGTTGCCAGTGGAAAAGAAATTTCAGAAGAAGATATAAGAGTTCACGAATCAAAGTAAATTTATTGGCTAGTCACATCTAGATTGGAAGGAAACGTGTAGTGAAACTTTTGGACCCAGTATACGATCAGTAGTTTCTTATTTCTCAATAAAAGGGTACGAACAATTTTGCTAGGACAGACATGTAAACATTATTATATTTTCAGAGATTGTTATTCGAGTACAATACATTTATATGGTTGAAAATAATGTAACTAATACTAGGATAGATAGCAAAGAAATTTTCGATATTGCCAAGTTCTCTTAAATTCAGTATTCTAACAAAATCTTGTATA

mRNA sequence

ATGGGAAGGAAACTGGATGCTTTACTGGGAAGGAATAGATATTTGAAGGCTTCCAAGTTTAAGACCCTTGCTAATATGGCCATTTCTAGGACTTCCATCCTAAAAAACCACCACCGCGCCCGTTGCTCCCTAGCTCGTGCCGATGTTCTTCAACTGTTGAATCTTAGTTACCAACATCGTGCTCAGCTTAGAGTTGAGATTGTGATCAAGGAGAATAACATGTTGGATGCTCTTGGAATGATAGAGGATTACTGCTGCCTTCTCGTAGAAAAGGTGGCTCTTCTTCAGATGAACAAGGAATGCCCTGGCGAAGTTAAGGAGGCGATATCGAGTCTGATATTTGCAGCTTCAAGGTGCGGAGAGTTTCCAGAACTTCAAGAGATTCGTCGGATTTTCGAGTTGAAGTTTGGTGCGGAGTTTGCAAGCAGTGCCATTGATTTACGCAACAACTGTGGTGTTAGTACCAAGATGATTCAAAAGTTTTCAACAAAACAGCCCAGTGCCGAAACTAAACTGAAAGTGCTAAAGGAAATTGCTTCAGAAAATGGCATTCCTCTCCATTTAGAGCAAGAATCACCAATAATCATCAAGGCAAAGCAAAACCAAAAGTTGGAAATCAAGAAAGAAGCAGATTTGTATAATCCTGAAGTCACAAACACCACAGATGATCTACATGAAGTTATTACAAGTGAAAAGATGGCATCGGAATTAGTGAAATCAAAGAAGTTTAAAGATGTAGCTAGTGCCACTGAAGAACCCTTCCAATCGGCAGCTTATGCAGCATTAGCAGCTAAAGCTGCAATCAACCTTTCAAAGTCCAAATCACAGGATATTGATTCAGATCATGAAGATGGCTCTCATCTACAAAAGAATGGAAAAGGCTCAGATTTGAATAGTTCTTATGAACCTAAGCTTAATACAAAAGAAGAAAGAATATCTTCCTGTAAGATTGACTATTCAGACGACAGATTTAGTTTTGAAAAGATTTGTCCTGTTGAACTTGAAAGTTGCAGTTCTCAATATGAAGATGCTGACATGGAAGAAACCAATCAAGAAGAGTTACCAAAAGAACCTGCCAAAGAAAGCACATCCTTTGCTCTAAAAGATTCAGATGTTAACAATATGAAGGAGCAATCATCAAAACCAAATTCAATCTTCTACAACGAGTCAGACGGCGACAAGATAACCAATACAGATAATGAAGTTGCCAGTGGAAAAGAAATTTCAGAAGAAGATATAAGAGTTCACGAATCAAAGTAA

Coding sequence (CDS)

ATGGGAAGGAAACTGGATGCTTTACTGGGAAGGAATAGATATTTGAAGGCTTCCAAGTTTAAGACCCTTGCTAATATGGCCATTTCTAGGACTTCCATCCTAAAAAACCACCACCGCGCCCGTTGCTCCCTAGCTCGTGCCGATGTTCTTCAACTGTTGAATCTTAGTTACCAACATCGTGCTCAGCTTAGAGTTGAGATTGTGATCAAGGAGAATAACATGTTGGATGCTCTTGGAATGATAGAGGATTACTGCTGCCTTCTCGTAGAAAAGGTGGCTCTTCTTCAGATGAACAAGGAATGCCCTGGCGAAGTTAAGGAGGCGATATCGAGTCTGATATTTGCAGCTTCAAGGTGCGGAGAGTTTCCAGAACTTCAAGAGATTCGTCGGATTTTCGAGTTGAAGTTTGGTGCGGAGTTTGCAAGCAGTGCCATTGATTTACGCAACAACTGTGGTGTTAGTACCAAGATGATTCAAAAGTTTTCAACAAAACAGCCCAGTGCCGAAACTAAACTGAAAGTGCTAAAGGAAATTGCTTCAGAAAATGGCATTCCTCTCCATTTAGAGCAAGAATCACCAATAATCATCAAGGCAAAGCAAAACCAAAAGTTGGAAATCAAGAAAGAAGCAGATTTGTATAATCCTGAAGTCACAAACACCACAGATGATCTACATGAAGTTATTACAAGTGAAAAGATGGCATCGGAATTAGTGAAATCAAAGAAGTTTAAAGATGTAGCTAGTGCCACTGAAGAACCCTTCCAATCGGCAGCTTATGCAGCATTAGCAGCTAAAGCTGCAATCAACCTTTCAAAGTCCAAATCACAGGATATTGATTCAGATCATGAAGATGGCTCTCATCTACAAAAGAATGGAAAAGGCTCAGATTTGAATAGTTCTTATGAACCTAAGCTTAATACAAAAGAAGAAAGAATATCTTCCTGTAAGATTGACTATTCAGACGACAGATTTAGTTTTGAAAAGATTTGTCCTGTTGAACTTGAAAGTTGCAGTTCTCAATATGAAGATGCTGACATGGAAGAAACCAATCAAGAAGAGTTACCAAAAGAACCTGCCAAAGAAAGCACATCCTTTGCTCTAAAAGATTCAGATGTTAACAATATGAAGGAGCAATCATCAAAACCAAATTCAATCTTCTACAACGAGTCAGACGGCGACAAGATAACCAATACAGATAATGAAGTTGCCAGTGGAAAAGAAATTTCAGAAGAAGATATAAGAGTTCACGAATCAAAGTAA

Protein sequence

MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCGEFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIASENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKSKKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSSYEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAKESTSFALKDSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHESK*
BLAST of Csa3G271380 vs. Swiss-Prot
Match: IST1_BOVIN (IST1 homolog OS=Bos taurus GN=IST1 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.3e-11
Identity = 51/186 (27.42%), Postives = 93/186 (50.00%), Query Frame = 1

Query: 15  LKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNM 74
           +KA + +    + I+R  +L+         AR ++   L      RA++RVE +I+E+ +
Sbjct: 6   IKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYL 65

Query: 75  LDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRC-GEFPELQEIRRIFE 134
           ++A+ ++E YC LL+ +  L+Q  KE    + E++S+LI+AA R   E  EL+ +     
Sbjct: 66  VEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLC 125

Query: 135 LKFGAEFASSAIDLRNNCG-VSTKMIQKFSTKQPSAETKLKVLKEIASENGIPLHLEQES 194
            K+  E+    +   N  G V+ +++ K S + P      + L EIA    +P   E +S
Sbjct: 126 AKYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVP--YEPDS 185

Query: 195 PIIIKA 199
            ++ +A
Sbjct: 186 VVMAEA 187

BLAST of Csa3G271380 vs. Swiss-Prot
Match: IST1_RAT (IST1 homolog OS=Rattus norvegicus GN=Ist1 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.3e-11
Identity = 51/185 (27.57%), Postives = 92/185 (49.73%), Query Frame = 1

Query: 16  KASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNML 75
           KA + +    + I+R  +L+         AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRC-GEFPELQEIRRIFEL 135
           +A+ ++E YC LL+ +  L+Q  KE    + E++S+LI+AA R   E  EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGAEFASSAIDLRNNCG-VSTKMIQKFSTKQPSAETKLKVLKEIASENGIPLHLEQESP 195
           K+  E+    +   N  G V+ +++ K S + P      + L EIA    +P   E +S 
Sbjct: 127 KYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVP--YEPDSV 186

Query: 196 IIIKA 199
           ++ +A
Sbjct: 187 VMAEA 187

BLAST of Csa3G271380 vs. Swiss-Prot
Match: IST1_HUMAN (IST1 homolog OS=Homo sapiens GN=IST1 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.3e-11
Identity = 51/185 (27.57%), Postives = 92/185 (49.73%), Query Frame = 1

Query: 16  KASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNML 75
           KA + +    + I+R  +L+         AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRC-GEFPELQEIRRIFEL 135
           +A+ ++E YC LL+ +  L+Q  KE    + E++S+LI+AA R   E  EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGAEFASSAIDLRNNCG-VSTKMIQKFSTKQPSAETKLKVLKEIASENGIPLHLEQESP 195
           K+  E+    +   N  G V+ +++ K S + P      + L EIA    +P   E +S 
Sbjct: 127 KYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVP--YEPDSV 186

Query: 196 IIIKA 199
           ++ +A
Sbjct: 187 VMAEA 187

BLAST of Csa3G271380 vs. Swiss-Prot
Match: IST1_MOUSE (IST1 homolog OS=Mus musculus GN=Ist1 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.3e-11
Identity = 51/185 (27.57%), Postives = 92/185 (49.73%), Query Frame = 1

Query: 16  KASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNML 75
           KA + +    + I+R  +L+         AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRC-GEFPELQEIRRIFEL 135
           +A+ ++E YC LL+ +  L+Q  KE    + E++S+LI+AA R   E  EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGAEFASSAIDLRNNCG-VSTKMIQKFSTKQPSAETKLKVLKEIASENGIPLHLEQESP 195
           K+  E+    +   N  G V+ +++ K S + P      + L EIA    +P   E +S 
Sbjct: 127 KYSKEYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVP--YEPDSV 186

Query: 196 IIIKA 199
           ++ +A
Sbjct: 187 VMAEA 187

BLAST of Csa3G271380 vs. Swiss-Prot
Match: IST1_PONAB (IST1 homolog OS=Pongo abelii GN=IST1 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.5e-10
Identity = 50/185 (27.03%), Postives = 91/185 (49.19%), Query Frame = 1

Query: 16  KASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKENNML 75
           KA + +    + I+R  +L+         AR ++   L      RA++RVE +I+E+ ++
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 76  DALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRC-GEFPELQEIRRIFEL 135
           +A+ ++E YC LL+ +  L+Q  KE    + E++S+LI+AA R   E  EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 136 KFGAEFASSAIDLRNNCG-VSTKMIQKFSTKQPSAETKLKVLKEIASENGIPLHLEQESP 195
           K+   +    +   N  G V+ +++ K S + P      + L EIA    +P   E +S 
Sbjct: 127 KYSKGYGK--LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVP--YEPDSV 186

Query: 196 IIIKA 199
           ++ +A
Sbjct: 187 VMAEA 187

BLAST of Csa3G271380 vs. TrEMBL
Match: A0A0A0L6Y2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G271380 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 6.2e-234
Identity = 419/419 (100.00%), Postives = 419/419 (100.00%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180

Query: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240
           ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS
Sbjct: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240

Query: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSS 300
           KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSS
Sbjct: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSS 300

Query: 301 YEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAK 360
           YEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAK
Sbjct: 301 YEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAK 360

Query: 361 ESTSFALKDSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHESK 420
           ESTSFALKDSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHESK
Sbjct: 361 ESTSFALKDSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHESK 419

BLAST of Csa3G271380 vs. TrEMBL
Match: A0A061EIJ4_THECC (Regulator of Vps4 activity in the MVB pathway protein, putative OS=Theobroma cacao GN=TCM_019471 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 2.0e-91
Identity = 205/425 (48.24%), Postives = 278/425 (65.41%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRN   KASKFKTLA +AISR +ILKN H+ R S AR+DV+ LLNL +Q R
Sbjct: 1   MGRKLDALLGRN--FKASKFKTLAKLAISRIAILKNQHQVRFSHARSDVIDLLNLGHQER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           A LRVE VIKE N++DAL ++E YC LL+E+V L+Q NKECP E+KEA SSL+FA+SRCG
Sbjct: 61  ALLRVEHVIKEQNLVDALTIMESYCQLLIERVMLIQKNKECPDELKEATSSLMFASSRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           EFPEL  IRR+    FG EF + A++LRNNC V  K++QK ST+QPS E KLKVLKEIAS
Sbjct: 121 EFPELLHIRRVVSSTFGKEFVARAVELRNNCSVHPKIVQKLSTRQPSLECKLKVLKEIAS 180

Query: 181 ENGIPLHLEQESPIIIKAK-----QNQKLEIKKEADLYNPEVTNTTDDL-HEVITSEKMA 240
           E GI LHLE +SP++++ K     +  +    K A+L +PE   T  +L  EV   EK++
Sbjct: 181 ERGIALHLEVDSPVVVEEKMDVHEKQNESTANKSANLDDPEYKYTAIELPREVNLDEKLS 240

Query: 241 SELVKSKKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNG-- 300
             +   KK++DVA+A +E F+SAAYAA AA+AA+ LS+++SQD D D E GS  +K G  
Sbjct: 241 ESVKARKKYRDVAAAAQEAFESAAYAAAAARAAVELSRTESQDFDPDDESGSSHKKGGPS 300

Query: 301 --KGS--DLNSSYEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEE 360
             KG+  D ++S  P+    E   ++  I++ ++   FEKI P+  ++ SS+ E  DM +
Sbjct: 301 LKKGAFYDSDASATPEFQGYE---AADGIEHPNNELGFEKIHPI--DNYSSESEGEDMTK 360

Query: 361 TNQ-------EELPKEP-AKESTSFALKDSDVNNMKEQSSKPNSIFYNESDGDK----IT 402
           TN        EE+ +    + + S +  DSDV+   E+    + +  NE  GD+    + 
Sbjct: 361 TNNGLELNEIEEIEQRVWIERALSTSSSDSDVDISTERKLSSDQLRQNEQLGDELAIDLV 418

BLAST of Csa3G271380 vs. TrEMBL
Match: K4BXC9_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.5e-78
Identity = 188/416 (45.19%), Postives = 257/416 (61.78%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRN   K SKFK  AN+AISR ++LKN  +ARCS+AR+DV+QLLNL Y  R
Sbjct: 1   MGRKLDALLGRN--FKTSKFKATANLAISRVTVLKNQRQARCSIARSDVVQLLNLGYHER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           A LRVE VIKE NMLD   M+E YC L +E++ L+Q  K CP E+KEAISSLIFAASRCG
Sbjct: 61  ALLRVEQVIKEQNMLDVFDMVEGYCLLTIERINLIQQEKICPEELKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           EFPELQE+R IF  +FG EFA+ A++LRNNCGV+TKMIQK ST+ PS E + KVLKEIA+
Sbjct: 121 EFPELQELRGIFTSRFGKEFAARAVELRNNCGVNTKMIQKLSTRMPSLEQRTKVLKEIAA 180

Query: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240
           EN I L +E+   I+++  + +++  K++ +  + E     D  HE              
Sbjct: 181 ENNIVLKIEE---IMLENTEEKEVTAKRKDEPEDHESVLPQDVDHEA------------R 240

Query: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSS 300
           +KFKDV +A +  F+SAAYAA AA+AA+ LS+S+S+D D   +  S  QK    SD    
Sbjct: 241 RKFKDVEAAAQAAFESAAYAAAAARAAVELSRSESRDTD---DPKSPSQKPRDVSDSRED 300

Query: 301 YEPKLNTKEERISSC--KIDYSDDRFSFEKICPVELESCSSQYEDAD-MEETNQEELPKE 360
            + + +  EE+ +    KI+ S + + F         S   Q+ D D +EE  Q++  K+
Sbjct: 301 LKSEFHAGEEKNNEGVEKIETSQN-YGF--------HSEVDQFSDGDEVEEVEQKKFEKQ 360

Query: 361 PAKESTSFALKDSDVNN--MKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEE 412
            +K S S +  D   ++  + E  S  + + Y+E D +     +N + S K    E
Sbjct: 361 -SKRSVSVSSSDISADDILVDEVKSPRHGVIYDEIDEE--VGEENRIPSLKHPKNE 384

BLAST of Csa3G271380 vs. TrEMBL
Match: A0A067F674_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g010606mg PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 7.4e-78
Identity = 180/419 (42.96%), Postives = 271/419 (64.68%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           M RKLD L GR+     SKFKT+  +AISR +IL+N H+AR   AR+DV +LLNL +  R
Sbjct: 1   MSRKLDRLFGRS--FNTSKFKTVTKLAISRAAILRNQHQARYYYARSDVTELLNLGHHER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           A  RVE +IKE NMLDALGMIEDYC LL+E+V L+Q NKECP E+KEAIS LIFA+SRCG
Sbjct: 61  ALTRVEYMIKELNMLDALGMIEDYCHLLIERVLLIQKNKECPDELKEAISGLIFASSRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           +FPELQ+IR IF+ ++GAEF + A++LRN CGV+ K++QK S+++ S E++LKVLKEI++
Sbjct: 121 DFPELQKIREIFKSRYGAEFDARAVELRNYCGVTPKIVQKLSSRRCSLESRLKVLKEIST 180

Query: 181 ENGIP-LHLEQESPIIIKAKQN--QKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASEL 240
           E GI  L+LE+++ + +K K +  QK     E   +N +  N  ++L+     EK++  +
Sbjct: 181 EMGIALLNLEEDTRVPVKEKSDIEQKQPKPMEPATFNDDAHNVPEELNR---DEKLSESV 240

Query: 241 VKSKKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDG-SHLQKNGKGSD 300
            + K ++DVA+A ++ F+SAAYAA AA+AA +LS+S+S+D +SD   G SH  +    SD
Sbjct: 241 KRRKYYRDVAAAAQDAFESAAYAAEAARAAFDLSRSESRDNNSDDFGGYSHGTEIASNSD 300

Query: 301 LNSSYEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPK 360
           ++ + +  L + ++ + + + + S  R  F++    +++S SS+  D D+ E  +E   K
Sbjct: 301 MSMTSD--LKSDKDAVINEEDEDSKSRMGFDQF--YDVDSFSSESGDEDVTEKTREIHFK 360

Query: 361 EPAKESTSFALK---------DSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGK 407
           E  +ES   A++         DSD N +        + + N+  G  + + D +  SGK
Sbjct: 361 E-LEESERTAVRERILSASSSDSDGNILSMTKKLAGAHYRNQEGGIAVASDDTDNDSGK 409

BLAST of Csa3G271380 vs. TrEMBL
Match: M1AZM5_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400012974 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 8.2e-77
Identity = 188/420 (44.76%), Postives = 254/420 (60.48%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRN   K SKFK  AN+AISR ++LKN  +ARCS+AR+DV+QLLNL +  R
Sbjct: 1   MGRKLDALLGRN--FKISKFKATANLAISRVTVLKNQRQARCSIARSDVVQLLNLGFHER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           A LRVE VIKE NMLD   MIE YC L +E+  L+Q  K CP E+KEAISSLIFAASRCG
Sbjct: 61  ALLRVEQVIKEQNMLDVFDMIEGYCLLTIERTNLIQQEKICPEELKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           EFPELQE+R IF  +FG EFA+ A++LRNNCGV+TKMIQK S + PS E + KVLKEIA+
Sbjct: 121 EFPELQELRAIFTSRFGKEFAARAVELRNNCGVNTKMIQKLSKRMPSLEQRTKVLKEIAA 180

Query: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKK--EADLYNPEVTNTTDDLHEVITSEKMASELV 240
           EN I L +E+   II++  + +++  K+  + + + P +    D  HE            
Sbjct: 181 ENNIVLKIEE---IILENTEEEEVIAKRKDQPEDHIPVLPQNVD--HEA----------- 240

Query: 241 KSKKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGS--D 300
             +K+KDVA+A +  F+SAAYAA+AA+AA+ LS+S+S+D+D D +  S   +N   S  D
Sbjct: 241 -RRKYKDVAAAAQAAFESAAYAAVAARAAVELSRSESRDLD-DPKSPSQKPRNVSDSRED 300

Query: 301 LNSSY---EPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEE 360
           L S +   E K N   E+I       +   + F         S   Q+ D D  E  ++ 
Sbjct: 301 LKSEFHAGEEKNNEGVEKIE------TSQNYGF--------HSEVDQFSDGDEVEELKQR 360

Query: 361 LPKEPAKESTSFALKDSDVNN--MKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEE 412
             +E  K S S +  D   ++  + E  S  + + Y+E D +     +N + S K    E
Sbjct: 361 KFEEQFKRSVSVSSSDISADDILVDEVKSPRHGVVYDEIDEE--VGEENRIPSLKRPKNE 384

BLAST of Csa3G271380 vs. TAIR10
Match: AT1G13340.1 (AT1G13340.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 216.9 bits (551), Expect = 2.5e-56
Identity = 144/385 (37.40%), Postives = 210/385 (54.55%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MG+KLDALLGR+   K +KFK+L  +A++R SILKN  +AR S A +DV +LL L     
Sbjct: 1   MGKKLDALLGRS--FKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEH 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           A  RV+ V+K+ N LD L  I  Y  L ++++ L + N++CP E+ EA+S L+FAASR G
Sbjct: 61  AYHRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           EFPELQEIR +   +FG + A+ +I+LR+NCGV  K+IQK ST+ P  E ++K LKEIA+
Sbjct: 121 EFPELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAA 180

Query: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240
           EN I L L+Q S         Q      +  L + +       L + +   K        
Sbjct: 181 ENNIVLKLDQASTSTEGTTNMQGTSDVSKTKLTSKDGRGEGYGLSDSVKRGK-------- 240

Query: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNG-------- 300
           KK+KDVA A +  F+SAA+AA AA+AA+ LS+   +  DS    G     +G        
Sbjct: 241 KKYKDVADAAQAAFESAAHAAEAAQAAVELSQFSPRGHDSPGNIGGENSFHGSENKESEQ 300

Query: 301 --KGSDLNSSYEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETN 360
             +G+D +S  E  + ++ +R  S   D  D      +  PV+L    +   D++ EET 
Sbjct: 301 EQEGNDDSSEGEGDVMSESKRSMSDSEDIIDVPVMSFREDPVKLLEKDTIIYDSE-EET- 360

Query: 361 QEELPKEPAKESTSFALKDSDVNNM 376
                 +P+ E+ + +    + N M
Sbjct: 361 ------QPSVETITISKSKDEQNRM 367

BLAST of Csa3G271380 vs. TAIR10
Match: AT4G35730.1 (AT4G35730.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 149.4 bits (376), Expect = 4.8e-36
Identity = 92/277 (33.21%), Postives = 141/277 (50.90%), Query Frame = 1

Query: 13  RYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKEN 72
           R   +SK KT A MA++R  +++N         R D+  LL       A++RVE VI+E 
Sbjct: 24  RGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQ 83

Query: 73  NMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCGEFPELQEIRRIF 132
           N+  A  +IE +C L+V ++ ++   K+CP ++KE I+SLIFAA RC E PEL ++R IF
Sbjct: 84  NIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIF 143

Query: 133 ELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIASENGIPLHLEQES 192
             K+G +F S+A DLR +CGV+  +I K S + P  E KLK++KEIA E  +     +  
Sbjct: 144 AKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETE 203

Query: 193 PIIIKAKQNQKLEIKKEADLYNPEVTNT--TDDLHEVITSEKMASELVKSKKFKDVASAT 252
             ++K ++      +K     +  V      + +       +  S +  +  + D  SA 
Sbjct: 204 QELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAA 263

Query: 253 EEPFQSAAYAALAAKAAINL-----SKSKSQDIDSDH 283
           E   + A  A  AA+ A  L     S +K   + SDH
Sbjct: 264 EAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDH 300

BLAST of Csa3G271380 vs. TAIR10
Match: AT1G25420.1 (AT1G25420.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 129.4 bits (324), Expect = 5.1e-30
Identity = 67/170 (39.41%), Postives = 104/170 (61.18%), Query Frame = 1

Query: 12  NRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKE 71
           NR +  +K KT  N+AI+R  +L+N    +    + ++   L    +  A++RVE VI+E
Sbjct: 9   NRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVIRE 68

Query: 72  NNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCGEFPELQEIRRI 131
            N+  A  ++E +C  ++ +V +L+  KECP E++EAI+S+IFAA RC E P+L +I+ +
Sbjct: 69  MNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNL 128

Query: 132 FELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIASE 182
           F  K+G EF   A +LR + GV+  +I+K S   PS   +LK+LKEIA E
Sbjct: 129 FGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQE 178

BLAST of Csa3G271380 vs. TAIR10
Match: AT2G19710.1 (AT2G19710.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 129.4 bits (324), Expect = 5.1e-30
Identity = 103/374 (27.54%), Postives = 173/374 (46.26%), Query Frame = 1

Query: 13  RYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKEN 72
           R  K +K KT   MA SR  ILKN    +    R ++ QLL       A++RVE V++E 
Sbjct: 7   RGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREE 66

Query: 73  NMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCGEFPELQEIRRIF 132
             + A  +I  YC LLV ++ +++  K CP ++KEA++S++FA+ R  + PEL EI + F
Sbjct: 67  KTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQF 126

Query: 133 ELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIASENGIPLHLEQES 192
             K+G +F++SA++LR + GVS  +++K S K P   TK+K+L  IA E+ +    E +S
Sbjct: 127 TTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVV--WEAQS 186

Query: 193 PIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKSKKFKDVASATEE 252
            +    K     E+   A+ + P  +   D     I S K     + +    +    + E
Sbjct: 187 FVESDPKDT---ELLNGANSFQPASSMNMD---SSINSNKEQPPNIHAPATVNAHHGSSE 246

Query: 253 ----PFQSAAYAALAAKAAINLSKSKSQD-IDSDHEDGSHLQKNGKGSDLNSSYEPKLNT 312
               P  S A    ++  + N++  K+ D   S           G+  + N  YE   + 
Sbjct: 247 RHHSPENSYANGGRSSSRSNNVTSGKADDYYHSKARPSRSRPDEGECRNPNHGYENSSSR 306

Query: 313 KEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAKESTSFAL 372
            +++     +D +D           E    +S    A  E +N+E + ++   +ST   +
Sbjct: 307 NKQKWEPEFVDSTD-----AARAAAEAAERASFAARAAAELSNKERMTRQ---DSTQSHI 364

Query: 373 KDSDVNNMKEQSSK 382
             + VN   E S +
Sbjct: 367 SSASVNLRNEPSHR 364

BLAST of Csa3G271380 vs. TAIR10
Match: AT4G29440.1 (AT4G29440.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 120.2 bits (300), Expect = 3.1e-27
Identity = 62/178 (34.83%), Postives = 101/178 (56.74%), Query Frame = 1

Query: 12  NRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHRAQLRVEIVIKE 71
           +R  K +K K    MA SR  ILKN    +    R ++  LL       A++RVE V++E
Sbjct: 6   HRSFKPAKCKIALQMAASRLKILKNKKDTQIKQLRRELAHLLESGQTQTAKIRVEHVVRE 65

Query: 72  NNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCGEFPELQEIRRI 131
              + A  ++  YC LLV ++ ++   K CP ++KEA++S+++A+ R  +  EL +I + 
Sbjct: 66  EKTVAAYELVGIYCELLVARLGVIDSQKTCPNDLKEAVASVLYASQRLTDVGELSDIVKH 125

Query: 132 FELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIASENGIPLHLE 190
           F  K+G +F S+AI L+ + GVS  +++K S K P   TK+K+L EIA+++ +    E
Sbjct: 126 FSAKYGKDFVSAAIGLQPDSGVSRLLVEKLSVKAPDGPTKIKILTEIATQHNVTWEAE 183

BLAST of Csa3G271380 vs. NCBI nr
Match: gi|778680668|ref|XP_011651369.1| (PREDICTED: uncharacterized protein LOC105434874 isoform X1 [Cucumis sativus])

HSP 1 Score: 817.8 bits (2111), Expect = 8.9e-234
Identity = 419/419 (100.00%), Postives = 419/419 (100.00%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180

Query: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240
           ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS
Sbjct: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240

Query: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSS 300
           KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSS
Sbjct: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSS 300

Query: 301 YEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAK 360
           YEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAK
Sbjct: 301 YEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAK 360

Query: 361 ESTSFALKDSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHESK 420
           ESTSFALKDSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHESK
Sbjct: 361 ESTSFALKDSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHESK 419

BLAST of Csa3G271380 vs. NCBI nr
Match: gi|659123287|ref|XP_008461584.1| (PREDICTED: uncharacterized protein LOC103500154 [Cucumis melo])

HSP 1 Score: 704.1 bits (1816), Expect = 1.4e-199
Identity = 367/420 (87.38%), Postives = 381/420 (90.71%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLK SKFKTLANMAISRTSILKN HRARCSLARADVLQLLNL YQHR
Sbjct: 1   MGRKLDALLGRNRYLKPSKFKTLANMAISRTSILKNQHRARCSLARADVLQLLNLGYQHR 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVAL QMNKECPGEVKEAISSLIFAASRCG
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALFQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFG EFASSAIDLRNNCGVS KMIQKFSTKQP+AETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGTEFASSAIDLRNNCGVSPKMIQKFSTKQPTAETKLKVLKEIAS 180

Query: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240
           ENGIPL+LE+E PII+K K NQKLE+KKEADLYNPEVTNTTDDLHEVITSEK+ASELVK+
Sbjct: 181 ENGIPLNLEEEPPIIVKEKLNQKLEVKKEADLYNPEVTNTTDDLHEVITSEKLASELVKA 240

Query: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNGKGSDLNSS 300
           KKFKDVASA EE FQSAA        AIN  KS+SQDIDSDHEDGSHLQKNGK S+LNSS
Sbjct: 241 KKFKDVASAAEEAFQSAA-------KAINFLKSESQDIDSDHEDGSHLQKNGKSSELNSS 300

Query: 301 YEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEETNQEELPKEPAK 360
           YE KLNTKE  I S KIDYSDDRFSFEKICPV LESCSSQYEDADMEETNQEELPKEPA 
Sbjct: 301 YESKLNTKEASIPSSKIDYSDDRFSFEKICPVGLESCSSQYEDADMEETNQEELPKEPAI 360

Query: 361 ESTSFALK-DSDVNNMKEQSSKPNSIFYNESDGDKITNTDNEVASGKEISEEDIRVHESK 420
           ESTS  L   SD+NN KEQSS+PNS FYNESDGDKI + DNEVASGKEISEE+I+VHE K
Sbjct: 361 ESTSSVLNLCSDINNTKEQSSEPNSTFYNESDGDKIISKDNEVASGKEISEENIKVHEPK 413

BLAST of Csa3G271380 vs. NCBI nr
Match: gi|778680671|ref|XP_011651370.1| (PREDICTED: uncharacterized protein LOC105434874 isoform X2 [Cucumis sativus])

HSP 1 Score: 556.6 bits (1433), Expect = 3.7e-155
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR
Sbjct: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG
Sbjct: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS
Sbjct: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180

Query: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240
           ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS
Sbjct: 181 ENGIPLHLEQESPIIIKAKQNQKLEIKKEADLYNPEVTNTTDDLHEVITSEKMASELVKS 240

Query: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHL 289
           KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHL
Sbjct: 241 KKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHL 288

BLAST of Csa3G271380 vs. NCBI nr
Match: gi|590652944|ref|XP_007033286.1| (Regulator of Vps4 activity in the MVB pathway protein, putative [Theobroma cacao])

HSP 1 Score: 344.4 bits (882), Expect = 2.9e-91
Identity = 205/425 (48.24%), Postives = 278/425 (65.41%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MGRKLDALLGRN   KASKFKTLA +AISR +ILKN H+ R S AR+DV+ LLNL +Q R
Sbjct: 1   MGRKLDALLGRN--FKASKFKTLAKLAISRIAILKNQHQVRFSHARSDVIDLLNLGHQER 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           A LRVE VIKE N++DAL ++E YC LL+E+V L+Q NKECP E+KEA SSL+FA+SRCG
Sbjct: 61  ALLRVEHVIKEQNLVDALTIMESYCQLLIERVMLIQKNKECPDELKEATSSLMFASSRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
           EFPEL  IRR+    FG EF + A++LRNNC V  K++QK ST+QPS E KLKVLKEIAS
Sbjct: 121 EFPELLHIRRVVSSTFGKEFVARAVELRNNCSVHPKIVQKLSTRQPSLECKLKVLKEIAS 180

Query: 181 ENGIPLHLEQESPIIIKAK-----QNQKLEIKKEADLYNPEVTNTTDDL-HEVITSEKMA 240
           E GI LHLE +SP++++ K     +  +    K A+L +PE   T  +L  EV   EK++
Sbjct: 181 ERGIALHLEVDSPVVVEEKMDVHEKQNESTANKSANLDDPEYKYTAIELPREVNLDEKLS 240

Query: 241 SELVKSKKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGSHLQKNG-- 300
             +   KK++DVA+A +E F+SAAYAA AA+AA+ LS+++SQD D D E GS  +K G  
Sbjct: 241 ESVKARKKYRDVAAAAQEAFESAAYAAAAARAAVELSRTESQDFDPDDESGSSHKKGGPS 300

Query: 301 --KGS--DLNSSYEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEE 360
             KG+  D ++S  P+    E   ++  I++ ++   FEKI P+  ++ SS+ E  DM +
Sbjct: 301 LKKGAFYDSDASATPEFQGYE---AADGIEHPNNELGFEKIHPI--DNYSSESEGEDMTK 360

Query: 361 TNQ-------EELPKEP-AKESTSFALKDSDVNNMKEQSSKPNSIFYNESDGDK----IT 402
           TN        EE+ +    + + S +  DSDV+   E+    + +  NE  GD+    + 
Sbjct: 361 TNNGLELNEIEEIEQRVWIERALSTSSSDSDVDISTERKLSSDQLRQNEQLGDELAIDLV 418

BLAST of Csa3G271380 vs. NCBI nr
Match: gi|359488181|ref|XP_002280096.2| (PREDICTED: uncharacterized protein LOC100249716 [Vitis vinifera])

HSP 1 Score: 318.5 bits (815), Expect = 1.7e-83
Identity = 196/419 (46.78%), Postives = 268/419 (63.96%), Query Frame = 1

Query: 1   MGRKLDALLGRNRYLKASKFKTLANMAISRTSILKNHHRARCSLARADVLQLLNLSYQHR 60
           MG+KLD LLGR+   K SK KTL N+AISR ++LKN  + RCS AR DV+QLLNL +Q  
Sbjct: 1   MGKKLDYLLGRS--FKISKLKTLVNLAISRLAVLKNQRQVRCSHARTDVIQLLNLGHQEP 60

Query: 61  AQLRVEIVIKENNMLDALGMIEDYCCLLVEKVALLQMNKECPGEVKEAISSLIFAASRCG 120
           A LRVE VIKE NMLD   MIE YC LL+E++ L Q NKECP E+KEA+SSLIFA SRCG
Sbjct: 61  ALLRVEYVIKEQNMLDVFLMIEAYCHLLIERITLFQ-NKECPDELKEAVSSLIFATSRCG 120

Query: 121 EFPELQEIRRIFELKFGAEFASSAIDLRNNCGVSTKMIQKFSTKQPSAETKLKVLKEIAS 180
            FPELQ+IR +F  +FG EFA+ A +L+NNCGV+ KMIQ+ ST+Q S E++LKVL EIAS
Sbjct: 121 GFPELQQIREMFVSRFGKEFAARAAELQNNCGVNLKMIQRLSTRQASLESRLKVLTEIAS 180

Query: 181 ENGIPLHLEQESPIIIKAKQ--NQK-----LEIKKEADLYNPEVTNTTDDLHEVITSEKM 240
           ENGI L LE+++ +  + KQ  NQK     LE  + A+L NPE+ +  +D  E+I   + 
Sbjct: 181 ENGITLSLEEDAALSTEEKQDVNQKQKRKQLESNESANLDNPELKDDNNDFPELINCGET 240

Query: 241 ASELVKSKKFKDVASATEEPFQSAAYAALAAKAAINLSKSKSQDIDSDHEDGS-HLQKNG 300
            SE VK++K++DVASA ++ F+SAAYAA AA+AA+ LS+++S D D D+  GS H ++  
Sbjct: 241 LSESVKARKYRDVASAAQDAFKSAAYAAAAARAAVELSRAESWDQDPDYYSGSKHQRRIM 300

Query: 301 KGSDLNSSYEPKLNTKEERISSCKIDYSDDRFSFEKICPVELESCSSQYEDADMEET--- 360
             SD +S+ + +++T         I+ S+D   F+ I P   ++ SS+ E  +ME+    
Sbjct: 301 SSSDGSSTSKLQVDT---NAFLQDIEKSNDGLGFDMIHPT--DNLSSESERDEMEDNLDR 360

Query: 361 ----NQEELPKEPAKEST-SFALKDSDVNNMKEQSSKPNSIFYNESD--GDKITNTDNE 402
                 +E  ++   E T S +  DSD  N+       N   +NE    G      DN+
Sbjct: 361 SHLQEMKECERKAGMERTLSGSSSDSDGGNLHGSQMSLNIPDWNEQSVKGTAFDGNDNQ 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
IST1_BOVIN1.3e-1127.42IST1 homolog OS=Bos taurus GN=IST1 PE=2 SV=1[more]
IST1_RAT2.3e-1127.57IST1 homolog OS=Rattus norvegicus GN=Ist1 PE=2 SV=1[more]
IST1_HUMAN2.3e-1127.57IST1 homolog OS=Homo sapiens GN=IST1 PE=1 SV=1[more]
IST1_MOUSE2.3e-1127.57IST1 homolog OS=Mus musculus GN=Ist1 PE=1 SV=1[more]
IST1_PONAB1.5e-1027.03IST1 homolog OS=Pongo abelii GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L6Y2_CUCSA6.2e-234100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G271380 PE=4 SV=1[more]
A0A061EIJ4_THECC2.0e-9148.24Regulator of Vps4 activity in the MVB pathway protein, putative OS=Theobroma cac... [more]
K4BXC9_SOLLC1.5e-7845.19Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1[more]
A0A067F674_CITSI7.4e-7842.96Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g010606mg PE=4 SV=1[more]
M1AZM5_SOLTU8.2e-7744.76Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400012974 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G13340.12.5e-5637.40 Regulator of Vps4 activity in the MVB pathway protein[more]
AT4G35730.14.8e-3633.21 Regulator of Vps4 activity in the MVB pathway protein[more]
AT1G25420.15.1e-3039.41 Regulator of Vps4 activity in the MVB pathway protein[more]
AT2G19710.15.1e-3027.54 Regulator of Vps4 activity in the MVB pathway protein[more]
AT4G29440.13.1e-2734.83 Regulator of Vps4 activity in the MVB pathway protein[more]
Match NameE-valueIdentityDescription
gi|778680668|ref|XP_011651369.1|8.9e-234100.00PREDICTED: uncharacterized protein LOC105434874 isoform X1 [Cucumis sativus][more]
gi|659123287|ref|XP_008461584.1|1.4e-19987.38PREDICTED: uncharacterized protein LOC103500154 [Cucumis melo][more]
gi|778680671|ref|XP_011651370.1|3.7e-155100.00PREDICTED: uncharacterized protein LOC105434874 isoform X2 [Cucumis sativus][more]
gi|590652944|ref|XP_007033286.1|2.9e-9148.24Regulator of Vps4 activity in the MVB pathway protein, putative [Theobroma cacao... [more]
gi|359488181|ref|XP_002280096.2|1.7e-8346.78PREDICTED: uncharacterized protein LOC100249716 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005061Ist1
Vocabulary: Biological Process
TermDefinition
GO:0015031protein transport
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
biological_process GO:0006950 response to stress
biological_process GO:0045087 innate immune response
biological_process GO:0031348 negative regulation of defense response
biological_process GO:0006979 response to oxidative stress
biological_process GO:0009697 salicylic acid biosynthetic process
biological_process GO:0009863 salicylic acid mediated signaling pathway
biological_process GO:0006952 defense response
biological_process GO:0050789 regulation of biological process
biological_process GO:0044763 single-organism cellular process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU128844cucumber EST collection version 3.0transcribed_cluster
CU158796cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa3G271380.1Csa3G271380.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU158796CU158796transcribed_cluster
CU128844CU128844transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161UNCHARACTERIZED DUF292coord: 2..310
score: 6.7E
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 21..185
score: 4.9
NoneNo IPR availablePANTHERPTHR12161:SF23REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 2..310
score: 6.7E