Lsi03G011210 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi03G011210
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPectinesterase
Locationchr03 : 21415776 .. 21419597 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACATTCCAAAGAGATAATTCTGTTAATAACAACATTGTTGTTATTGTTTTGTTTCAATGTTTCAAAGGCATTGGATTGTGAGTTGAATTATAATGATCCTCATAAAGTTGCATATACTATTGTTGTTGACAAATCAGGGAGAGGAAACTTCACAACAATCCAAAGTGCCATTGATTCAATTCCTTTGCAAAATACCAAATGGATTAGAGTTCAAATTTTTCCTGGCATATATTCGTAAGATATATTATTTATTTACGTTTAATTTTATTAATAAATATCATTTTATTTTCTATATTTTAAATTTTATTCGATTTTCGTCTTTCTACTTCATTTGTTTTCAATAAAACTTAAAATCAGTCCTTACAATTAGTTTAGTGTTAAAACTTTCAAACATATAATTTAGTTTTCAATTGTCTGATCCTTCTCGTTCTATAAAGCATAACCACACCACATTACTTCTCTCTCTTGCATTGCATAAACTATTATTTAACCTATTTAAATAAAACGAAGTAACTTTAAACCCAAAACCTAAAGACTAAAAAGGTTTTTTTTTTTTTTTCTAAGAAAAACTGAACTGAAAAAAGCAAATTTTAAGATTGATTGAAAGTATAGGTTGAACATTTGAAAGTATTATTTAGGATCTCACGTTAAGAAAATAAGGAAGACCTCATAATCTTTATAAGATACAAAAATTACTCTCCTAGTTGTCAATTAGTTTTCGAATGAAATCTCACATGTTTATCTAATGTTGTATTAGAGTTCAAGAAGCCCAAATGAATATTCGGTCCAAGATGAAACGAAACTCAAAATACAGAAATAATTTAGTGATCATTTATTGTTTAGGATAGTTAATTCAAATGGGTTTGAGTTTTTTTTTTTTTTCGGAAATTACTATAAACAGAAAAAATATCAAACTATTTACAAATATAGAAAAATAAGCAAAACTATTTACAAATATGGAAAAATTTCACTATCTATCAGTGATAGTCCGTGATAGAATTCTATCGCGGTCTATCACTCAAGCGGTAGAATTTATTTGCTTAAGTGATAGAAAGCGATAGAGTTCTATCGCGTTCTATCAATGATAGACTGTAGAATTTTTCTATATTTGTAAATAGTTCGCTCATTTTTCTATATCTGAAAAGAGCTTTTTTTTTTTTTTCGGAAATTACTAAAAACAGAAAAAATATCAAACTATTTACAAATATGGAAAAATTTCACTATCTATCAGTGATAGTCCGTGATAGAATTCTATCGCGGTCTATCACTCAAGCGGTAGAATTTATTTGCTTAAGTGATAGAAAGCGATAGAGTTCTATCGCGTTCTATCAATGATAGACTGTAGAATTTTTCTATATTTGTAAATAGTTTTGCTCATTTTTCTATATTTGAAAAGAGCTTTTTTTTTTTTTTCTTTTTTTTTTTTTTCTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTCTTCATTTGTATGTTTGTACTTTAGTTGAGTGAGTCTCTCAGCTAAATACATGAGTTTTTTTTTCTTTTTGTCAACCTATTTTTTAGAAAAATTAAAATAGTTTTGACAGATAAAAAATGGATTTTGTTTTAATTTTGATATATTAACAGGGAAAAGGTGATGATTCCCCCAGAAAAACCATGCATATTTCTAGATGGTGGTGGGCGTAGAGCCACTGAAATTCAATGGAATGACCATACAACAACAGCCACTAGTGCCACCTTCACTTCTTCTGCAGAAAACCTTGTAGTACAAGGCATCACTTTTAGGGTATAACTATTTAATTTCCATTTAACTTTCACATTTATATTTATATTTTCTTGTTTTAAACTTTATACTAAAAATTAAATCTACTTCATTTCTTATATTATCATTCCATTCCTTTACTTACATTTTATGTCTTTGCACATTAGCTAAATAAACTTTTACTTCTCCTAAGGAGTCTCTATTTATGATGCAATATTCTATACACTACGTTTAATAATTACAATAATTCTTTTTTAAAAATAAGAAAAATATATTTTTTGTCTCTAGGTTTTAGATCTAGTTTTCATTTGGACCTTAGGTTTCAAGATGTTACATATTTAGTCCTTAAGTTTTGAATTTGTTTCAATTTAGTCCGTAGACTTCAAAATGTTATAATTTTACCCTTGAGATTTGAGTTTTGTTTCAATTTGGTCCCTAGGTTTCAAGATTTATACTTTAACCCCGATTTTTCACTAAATACTCACTTTCCATCTTTAGTTTTAATATCTTTTAATTAATTTAAAAGAATTAAAAGAATCATAGTTAATTAAATTAACTATTTTTTTTATCACTATTAGAATTAAATTCAAATTTTCATTGAGAAATTATTATAAACAGAAAAAGTATCAAACTATTTACAAATATAGAAAAATTTCACTGTCTATCAGCGATAGATCGCAATAGAATTCATTCACTTGAGCGATAGTGTTCTATCGCTGATAAACACTGAATTTTTTTTATATTTGTAAATAGTTCGCTCATTTTTCTATATTTGAAAATAGTTCATTTTCATTTCATAATTATTTTAAGCAATTAATTAATAGACATTCATGTCAATGCCTGAACGCGAGTATTTAATGAAAAATCGAGGTTAAAGGTGTAAATCTTGAAACCAATGGGTCAAATTGAAACAAAACTCAAATCTCATGGGTAGAATTGTAACATTTATAAACTTAGGGACTAAGTTGAAACAAAACTCAAACATTAAGAACTAAATTCGGAACATTTTGAAACTTAGAAACCAAATAGAAATTAATTCCAAAACTTTAAGGACCAAAAAAGTTTCTCACTTTAAAGAAAATCATACAAACATGTCAAAGCATAAGATTATTGTAAATGGATATGGAATTAGATGAAAATAAGATTTAAATACTTAAAGGGGTTTGTAAATGAAATTGGCAGAATACTCATAATGCAGCAGGAAATGTGAGGAGACGTGAAGATATAGCACCAGCAATTGCAGCTCGCATAGAAGGAGACAAAGGGATATTCCATAAATGTGGCTTCATTGGTTTGCAAGATACTTTGTGGGATGGACATGGCCGTCATCTCTTCACCCGATGCTACATTGAAGGTGTCATTGATGTCATCTCTGGTTTTGCTCAGTCTATCTACCAGGTTAATTAACTTCTAAATCAAATCTTTCATTTAGGCTTTATTCATACTTGCAAGTGTATGAGTCAGTTTACACACATGCTTAAACCAATCGTTAATTGAAATGTTGATGATGATTATTGTTTTGGTGTTGTGTTATTGCAGAAATGTGTGATAAATATACCAATCAATGTGTATGCTCCAATACTAAATCTTGGGTTCATAACAGCTCAAGGAAAGAATGATGCAAATGAAAGCAGTGGGTTTGTATTCATTGGATGCACAGTGATGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTATCTTTTCTACCATCTTGCATTAACCCTGCTGGTTGGAATTCTTGGGAACAACTTGGCCATGAGTAACTTTCTTTTCGTTTCATTTTTATTCCCACTTTTGCTTTATAAATTTAATTACTTACTTCTTCAAAAGTATATGTCTACCACGTTTATCTAATGTGGTATCAAATCATGTAGGATGGATTTGACATATGCAGAGAGCCGTTGTATTGGACCAGGAGCAGACACTTCAAAACGTGTGCCTTGGCTTAAGAGACTGGTTGGAGTTGAGATCAAACACTTTACACATATTTCTTATATTGATCAAGAAGGTTGGACTTCAAGGCTTCCTGTATTTTCGTAA

mRNA sequence

ATGGAACATTCCAAAGAGATAATTCTGTTAATAACAACATTGTTGTTATTGTTTTGTTTCAATGTTTCAAAGGCATTGGATTGTGAGTTGAATTATAATGATCCTCATAAAGTTGCATATACTATTGTTGTTGACAAATCAGGGAGAGGAAACTTCACAACAATCCAAAGTGCCATTGATTCAATTCCTTTGCAAAATACCAAATGGATTAGAGTTCAAATTTTTCCTGGCATATATTCGGAAAAGGTGATGATTCCCCCAGAAAAACCATGCATATTTCTAGATGGTGGTGGGCGTAGAGCCACTGAAATTCAATGGAATGACCATACAACAACAGCCACTAGTGCCACCTTCACTTCTTCTGCAGAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTCATAATGCAGCAGGAAATGTGAGGAGACGTGAAGATATAGCACCAGCAATTGCAGCTCGCATAGAAGGAGACAAAGGGATATTCCATAAATGTGGCTTCATTGGTTTGCAAGATACTTTGTGGGATGGACATGGCCGTCATCTCTTCACCCGATGCTACATTGAAGGTGTCATTGATGTCATCTCTGGTTTTGCTCAGTCTATCTACCAGAAATGTGTGATAAATATACCAATCAATGTGTATGCTCCAATACTAAATCTTGGGTTCATAACAGCTCAAGGAAAGAATGATGCAAATGAAAGCAGTGGGTTTGTATTCATTGGATGCACAGTGATGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTATCTTTTCTACCATCTTGCATTAACCCTGCTGGTTGGAATTCTTGGGAACAACTTGGCCATGAGATGGATTTGACATATGCAGAGAGCCGTTGTATTGGACCAGGAGCAGACACTTCAAAACGTGTGCCTTGGCTTAAGAGACTGGTTGGAGTTGAGATCAAACACTTTACACATATTTCTTATATTGATCAAGAAGGTTGGACTTCAAGGCTTCCTGTATTTTCGTAA

Coding sequence (CDS)

ATGGAACATTCCAAAGAGATAATTCTGTTAATAACAACATTGTTGTTATTGTTTTGTTTCAATGTTTCAAAGGCATTGGATTGTGAGTTGAATTATAATGATCCTCATAAAGTTGCATATACTATTGTTGTTGACAAATCAGGGAGAGGAAACTTCACAACAATCCAAAGTGCCATTGATTCAATTCCTTTGCAAAATACCAAATGGATTAGAGTTCAAATTTTTCCTGGCATATATTCGGAAAAGGTGATGATTCCCCCAGAAAAACCATGCATATTTCTAGATGGTGGTGGGCGTAGAGCCACTGAAATTCAATGGAATGACCATACAACAACAGCCACTAGTGCCACCTTCACTTCTTCTGCAGAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTCATAATGCAGCAGGAAATGTGAGGAGACGTGAAGATATAGCACCAGCAATTGCAGCTCGCATAGAAGGAGACAAAGGGATATTCCATAAATGTGGCTTCATTGGTTTGCAAGATACTTTGTGGGATGGACATGGCCGTCATCTCTTCACCCGATGCTACATTGAAGGTGTCATTGATGTCATCTCTGGTTTTGCTCAGTCTATCTACCAGAAATGTGTGATAAATATACCAATCAATGTGTATGCTCCAATACTAAATCTTGGGTTCATAACAGCTCAAGGAAAGAATGATGCAAATGAAAGCAGTGGGTTTGTATTCATTGGATGCACAGTGATGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTATCTTTTCTACCATCTTGCATTAACCCTGCTGGTTGGAATTCTTGGGAACAACTTGGCCATGAGATGGATTTGACATATGCAGAGAGCCGTTGTATTGGACCAGGAGCAGACACTTCAAAACGTGTGCCTTGGCTTAAGAGACTGGTTGGAGTTGAGATCAAACACTTTACACATATTTCTTATATTGATCAAGAAGGTTGGACTTCAAGGCTTCCTGTATTTTCGTAA

Protein sequence

MEHSKEIILLITTLLLLFCFNVSKALDCELNYNDPHKVAYTIVVDKSGRGNFTTIQSAIDSIPLQNTKWIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVRRREDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIKHFTHISYIDQEGWTSRLPVFS
BLAST of Lsi03G011210 vs. Swiss-Prot
Match: PME55_ARATH (Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.5e-85
Identity = 145/311 (46.62%), Postives = 203/311 (65.27%), Query Frame = 1

Query: 35  PHKVA---YTIVVDKSGRGNFTTIQSAIDSIPLQNTKWIRVQIFPGIYSEKVMIPPEKPC 94
           PH +    + ++VD+SG GNFTTIQ AIDS+P+ NT W  + +  G+Y EK+ IP +KP 
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 95  IFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVR-RREDIAP 154
           I + G G+R+T ++W+DH + A S TF + A+N VV+ ITF N++N   N +  +    P
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVP 138

Query: 155 AIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVIN 214
           A+AA I GDK  F+  GF G+QDTLWD  GRH F RC I+G +D I G  QSIYQ CVI 
Sbjct: 139 AVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQ 198

Query: 215 IPINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSF 274
           +      P +  G+ITAQG+ +AN+++GFVFI C V G G  +LGRA+RP+S VIF+ S 
Sbjct: 199 VLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSN 258

Query: 275 LPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIKHFTHISYI 334
           L   ++P GW  W   G+E  LTYAE  C G G++TS+R  W+K+L    ++H   +S+I
Sbjct: 259 LTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFI 318

Query: 335 DQEGWTSRLPV 342
           ++ GW   LP+
Sbjct: 319 NRGGWVEDLPI 328

BLAST of Lsi03G011210 vs. Swiss-Prot
Match: PME66_ARATH (Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2)

HSP 1 Score: 312.0 bits (798), Expect = 8.1e-84
Identity = 155/333 (46.55%), Postives = 212/333 (63.66%), Query Frame = 1

Query: 9   LLITTLLLLFCFNVSKALDCELNYNDPHKVAYTIVVDKSGRGNFTTIQSAIDSIPLQNTK 68
           ++   ++ LF +  S A +         ++AYTI VD +G GNFTT+QSAIDSI   N  
Sbjct: 11  IVCMVVMTLFVYGYSAAAE---------QIAYTITVDLNGGGNFTTVQSAIDSISPPNHN 70

Query: 69  WIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQ 128
           WIRV    GIY EKV IP EK  I+L G G   T I+++DH  T  SATFT+ A+++V+ 
Sbjct: 71  WIRVFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVIS 130

Query: 129 GITFRNTHNAAGNVRRREDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCY 188
           GITF+NT+N   N +R  +I PA+AAR+ GD+ +     F+GLQDTL+DG GRH + RC 
Sbjct: 131 GITFKNTYNIVPNNKR--EIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCI 190

Query: 189 IEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMG 248
           I G ID I G+ QS++++C +N+ + +YAP    G ITA  +   ++  GFVF  CTV G
Sbjct: 191 ISGGIDFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTG 250

Query: 249 SGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSK 308
            G   LGRA+   + VIF  S L   + P GW++W   G+E DLT+ E+ C G GADTS+
Sbjct: 251 VGKTLLGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQ 310

Query: 309 RVPWLKRLVGVEIKHFTHISYIDQEGWTSRLPV 342
           RVPWLK+L   E+  F  +S+IDQ+GW SR P+
Sbjct: 311 RVPWLKKLSLSEVDGFASVSFIDQDGWISRFPI 332

BLAST of Lsi03G011210 vs. Swiss-Prot
Match: PME10_ARATH (Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.4e-80
Identity = 146/305 (47.87%), Postives = 194/305 (63.61%), Query Frame = 1

Query: 37  KVAYTIVVDKSGRGNFTTIQSAIDSIPLQNTKWIRVQIFPGIYSEKVMIPPEKPCIFLDG 96
           ++A TI+V+ +    F T+QSAIDSIPLQN  WIR+ I  GIYSEKV IP  K  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 97  GGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVRRREDIAPAIAARI 156
           GG   T I + DH  T TSATFTS   N+++ GITF+N +N A +        PA+AA +
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 157 EGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVINIPINVY 216
            GDK       F G QDTL+D +GRH + RC I G ID I G AQSI++ C + + + +Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 217 APILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCIN 276
            P    G ITAQG++   +  GFVF  CTVMGSG   LGRA++ +S VIF+ S     I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 277 PAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIKHFTHISYIDQEGWT 336
           P GW++W+  G E  +T+ E  C G GADTSKRVPWL +    ++  FT++++ID+EGW 
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWL 335

Query: 337 SRLPV 342
           SRLP+
Sbjct: 336 SRLPI 337

BLAST of Lsi03G011210 vs. Swiss-Prot
Match: PME29_ARATH (Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.7e-79
Identity = 139/301 (46.18%), Postives = 194/301 (64.45%), Query Frame = 1

Query: 42  IVVDKSGRGNFTTIQSAIDSIPLQNTKWIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRA 101
           + VD+SG GNFTTIQ AIDS+P+ N  W  + +  G+Y EK+ IP EKP I L G G+R 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 102 TEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVRRREDI-APAIAARIEGDK 161
           T ++W+DH + A S TF++ A+N VV+ ITF N++N     +  ++   PA+AA I GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 162 GIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVINIPINVYAPIL 221
             F+  GF G+QDTLWD  GRH F RC I+G +D I G  QSIYQ CVI +      P L
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPGL 213

Query: 222 NLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGW 281
             G+ITAQG+ +  +++GF+FI C V G+G  FLGR +R +S VIF+ S L   + P GW
Sbjct: 214 -AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGW 273

Query: 282 NSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIKHFTHISYIDQEGWTSRLP 341
           ++W  +GHE  L +AE  C G GA+  +RV W+K+L    I++   +S+I++ GW   LP
Sbjct: 274 DAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLP 333

BLAST of Lsi03G011210 vs. Swiss-Prot
Match: PME52_ARATH (Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2)

HSP 1 Score: 237.3 bits (604), Expect = 2.5e-61
Identity = 115/252 (45.63%), Postives = 158/252 (62.70%), Query Frame = 1

Query: 96  GGGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVRRRE----DIAPA 155
           G G+R T I +N H  T  S+TFTS   ++VV+ ++  NT+N   ++ +      DI PA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 156 IAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVINI 215
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G  QS+Y+ C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 216 PINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFL 275
                A  ++ G+ITAQG++  ++ SGFVF+  +V GS +V+LGRAY PFS VIF  + L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 276 PSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIK-HFTHISYI 335
            S ++P GW SW   G+EM  TYAE  C G G+D S+RVPW+ +L     K  F+  ++I
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 336 DQEGWTSRLPVF 343
           DQ+ W S +P F
Sbjct: 282 DQDQWISNIPRF 293

BLAST of Lsi03G011210 vs. TrEMBL
Match: A0A0A0LB12_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 9.5e-156
Identity = 269/348 (77.30%), Postives = 299/348 (85.92%), Query Frame = 1

Query: 1   MEH--SKEIIL-LITTLLLLFCFNVSKALD-CELNYNDPHKVAYTIVVDKSGRGNFTTIQ 60
           MEH  SKEI L +ITTL++LFC  VSKALD CELNYNDPHKVAYTIVVDK G+GNFTTIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAIDSIPLQNTKWIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSA 120
           SAI+S+P Q+TKWIR+QI PGIY+EKV IP EK CIFLDG G + TEI WNDH TTA S 
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTSSAENLVVQGITFRNTHNAAGNVRRREDIAPAIAARIEGDKGIFHKCGFIGLQDTLW 180
           TFT+SA+NLVVQGITFRNT+NA G+V RREDI PA+AA I+GDK IFHKCGFIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANES 240
           DG GRHLFT+CYIEGVIDVISGF QSIY++CVINIP+N YAP+LN GFITAQGK + NES
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAE 300
           SGFVF+ C V GSGNVFLGRAYRPFSTVIFHL FLPSCINPAGWNSW Q GHE DLTY+E
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 SRCIGPGADTSKRVPWLKRLVGVEIKHFTHISYIDQEG-WTSRLPVFS 344
           +RCIGPGADTS RVPW+ RL    I+ FT ISYID +G WTSR+P+ S
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS 348

BLAST of Lsi03G011210 vs. TrEMBL
Match: A0A067HG98_CITSI (Pectinesterase OS=Citrus sinensis GN=CISIN_1g043974mg PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.2e-102
Identity = 182/334 (54.49%), Postives = 226/334 (67.66%), Query Frame = 1

Query: 10  LITTLLLLFCFNVSKALDCELNYNDPHKVAYTIVVDKSGRGNFTTIQSAIDSIPLQNTKW 69
           L    LLL   NVS ALDC+LN  +P KVAYTI V +SG GNFT IQ AI+ IP+ N +W
Sbjct: 8   LFAVTLLLLSSNVSTALDCKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPVNNDQW 67

Query: 70  IRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQG 129
           IR+QI P IY EKV IP  KPCIFL+G   R T+IQW DH TT +S TFTS +EN+V +G
Sbjct: 68  IRIQISPEIYQEKVTIPQNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSENVVAKG 127

Query: 130 ITFRNTHNAAGNVRR---REDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTR 189
           I F+NT+N   + R+   R ++  A+AARI GDK  F+ CGF GLQDTL+D HGRH F +
Sbjct: 128 ILFQNTYNVPPSYRQNQERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQ 187

Query: 190 CYIEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFVFIGCTV 249
           CYIEG ID I G  QSIY  C +N+ +  Y P  + G+ITAQG+N  ++ SGFVF  C  
Sbjct: 188 CYIEGAIDFIFGNGQSIYHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVFTSCQF 247

Query: 250 MGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADT 309
           +GSG  +LGRAY  FS VI   S L   + P GWN+W  + HE ++ YAE+RC G GADT
Sbjct: 248 LGSGKAYLGRAYGAFSRVIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRGAGADT 307

Query: 310 SKRVPWLKRLVGVEIKHFTHISYIDQEGWTSRLP 341
           SKRV W K L   E+K FT  S+ID +GW ++LP
Sbjct: 308 SKRVSWEKHLSVDEVKKFTDSSFIDHDGWIAKLP 341

BLAST of Lsi03G011210 vs. TrEMBL
Match: A0A061DSC1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 3.9e-101
Identity = 178/343 (51.90%), Postives = 234/343 (68.22%), Query Frame = 1

Query: 1   MEHSKEIILLITTLLLLFCFNVSKALDCELNYNDPHKVAYTIVVDKSGRGNFTTIQSAID 60
           M+  + I+ LI  +LL F  ++SKAL+C+LN  +  KVA+TI VD+SG+G+F TIQ+AID
Sbjct: 17  MQFLQHILALIFIVLLYF--SISKALECQLNNANKFKVAHTIRVDQSGKGDFKTIQNAID 76

Query: 61  SIPLQNTKWIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTS 120
           SIP  N KWIR+ I PG + EKV IP  KPCIFL+G G + T I+W DH  T  SATFTS
Sbjct: 77  SIPSNNDKWIRILISPGHFREKVTIPANKPCIFLEGAGSKLTSIEWGDHMDTCDSATFTS 136

Query: 121 SAENLVVQGITFRNTHNAAGNVRRREDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHG 180
           S +N+V +GI+F+N +N       ++ + PA+A RI GDK  F+ C F GLQDTLWD  G
Sbjct: 137 SPDNIVAKGISFKNIYNIPSGPDAKKKVEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQG 196

Query: 181 RHLFTRCYIEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFV 240
           RH F  CYIEG +D I G  QSIY+KC IN+    YAP    G+ITAQG+N +++ SGFV
Sbjct: 197 RHYFYNCYIEGAVDFIFGSGQSIYEKCEINLTAGKYAPEYPNGYITAQGRNSSDDPSGFV 256

Query: 241 FIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCI 300
           F  C   G G  +LGRAY  +S VI + S L   I  +GW++W  + HE +L Y E+ C 
Sbjct: 257 FKYCVFTGYGKTYLGRAYGAYSRVIIYKSVLSDAILASGWDAWRYVHHEGNLIYVEADCK 316

Query: 301 GPGADTSKRVPWLKRLVGVEIKHFTHISYIDQEGWTSRLPVFS 344
           GPGA+TSKRVPWLK+L   ++K F ++SYID+EGW ++LP  S
Sbjct: 317 GPGANTSKRVPWLKKLGASQLKKFLNLSYIDKEGWIAKLPKVS 357

BLAST of Lsi03G011210 vs. TrEMBL
Match: V4VWS4_9ROSI (Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 9.6e-100
Identity = 174/318 (54.72%), Postives = 218/318 (68.55%), Query Frame = 1

Query: 26  LDCELNYNDPHKVAYTIVVDKSGRGNFTTIQSAIDSIPLQNTKWIRVQIFPGIYSEKVMI 85
           LDC+LN  +P KVAYTI V +SG GNFT IQ AI+ IP+ N +WIR+QI P IY EKV I
Sbjct: 1   LDCKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPVNNDQWIRIQISPEIYQEKVTI 60

Query: 86  PPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVRR- 145
           P  KPCIFL+G   R T+IQW DH TT +S TFTS +EN+V +GI F+NT+N   + R+ 
Sbjct: 61  PQNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSENVVAKGILFQNTYNVPPSYRQN 120

Query: 146 --REDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQS 205
             R ++  A+AARI GDK  F+ CGF GLQDTL+D HGRH F +CYIEG ID I G  QS
Sbjct: 121 QERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQCYIEGAIDFIFGNGQS 180

Query: 206 IYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFS 265
           IY  C +N+ +  Y P  + G+ITAQG+N  ++ SGFVF  C  +GSG  +LGRAY  FS
Sbjct: 181 IYHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVFTSCQFLGSGKAYLGRAYGAFS 240

Query: 266 TVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIK 325
            VI   S L   + P GWN+W  + HE ++ YAE+RC G GADTSKRV W K L   E+K
Sbjct: 241 RVIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRGAGADTSKRVSWEKHLSVDEVK 300

Query: 326 HFTHISYIDQEGWTSRLP 341
            FT  S+ID +GW ++LP
Sbjct: 301 KFTDSSFIDHDGWIAKLP 318

BLAST of Lsi03G011210 vs. TrEMBL
Match: A0A0D2U2R3_GOSRA (Pectinesterase OS=Gossypium raimondii GN=B456_008G138100 PE=4 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.7e-96
Identity = 171/337 (50.74%), Postives = 227/337 (67.36%), Query Frame = 1

Query: 7   IILLITTLLLLFCFNVSKALDCELNY-NDPHKVAYTIVVDKSGRGNFTTIQSAIDSIPLQ 66
           +++ +  +LLL CF++ +A +CE +  N   KVA TI VD+SG+G+F T+Q AIDSIP  
Sbjct: 4   LLVFMLIVLLLVCFSICEAFECEFDAENHQFKVADTIRVDQSGKGDFKTVQKAIDSIPSN 63

Query: 67  NTKWIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTSSAENL 126
           N KWIR+ I PG++ EKV IP +KPCIFL+G GR  T I+WN H  T  SAT TS  +++
Sbjct: 64  NKKWIRILISPGVFREKVTIPYDKPCIFLEGAGRLLTRIEWNSHMRTCDSATLTSFPDSI 123

Query: 127 VVQGITFRNTHNA--AGNVRRREDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHL 186
           V +GITF+N +N   A     +  + PA+AARI GDK  F+ C F GLQDTLWD  GRH 
Sbjct: 124 VAKGITFKNFYNVPLATIPNIKNTVKPALAARIYGDKSAFYNCAFFGLQDTLWDVQGRHY 183

Query: 187 FTRCYIEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFVFIG 246
           F  CYIEG ID I G  QSIY+ C IN+ I  YAP    G+ITAQG+N +++ SGFVF  
Sbjct: 184 FKNCYIEGAIDFIFGSGQSIYESCEINLTIGKYAPQYPNGYITAQGRNSSDDPSGFVFKS 243

Query: 247 CTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCIGPG 306
           C   G+G  +LGRAY  +S VI + S +   I  +GW++W  + HE +L YAE+ C G G
Sbjct: 244 CVFSGTGKTYLGRAYGAYSRVIIYKSVMTDIIVASGWDAWTYVHHEGNLMYAENSCRGAG 303

Query: 307 ADTSKRVPWLKRLVGVEIKHFTHISYIDQEGWTSRLP 341
           A+TSKRVPWL++L   ++  F +ISYID+EGW  +LP
Sbjct: 304 ANTSKRVPWLRKLSASQLSQFVNISYIDKEGWIDKLP 340

BLAST of Lsi03G011210 vs. TAIR10
Match: AT5G18990.1 (AT5G18990.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 317.0 bits (811), Expect = 1.4e-86
Identity = 145/311 (46.62%), Postives = 203/311 (65.27%), Query Frame = 1

Query: 35  PHKVA---YTIVVDKSGRGNFTTIQSAIDSIPLQNTKWIRVQIFPGIYSEKVMIPPEKPC 94
           PH +    + ++VD+SG GNFTTIQ AIDS+P+ NT W  + +  G+Y EK+ IP +KP 
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 95  IFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVR-RREDIAP 154
           I + G G+R+T ++W+DH + A S TF + A+N VV+ ITF N++N   N +  +    P
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVP 138

Query: 155 AIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVIN 214
           A+AA I GDK  F+  GF G+QDTLWD  GRH F RC I+G +D I G  QSIYQ CVI 
Sbjct: 139 AVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQ 198

Query: 215 IPINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSF 274
           +      P +  G+ITAQG+ +AN+++GFVFI C V G G  +LGRA+RP+S VIF+ S 
Sbjct: 199 VLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSN 258

Query: 275 LPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIKHFTHISYI 334
           L   ++P GW  W   G+E  LTYAE  C G G++TS+R  W+K+L    ++H   +S+I
Sbjct: 259 LTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFI 318

Query: 335 DQEGWTSRLPV 342
           ++ GW   LP+
Sbjct: 319 NRGGWVEDLPI 328

BLAST of Lsi03G011210 vs. TAIR10
Match: AT2G47280.1 (AT2G47280.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 312.0 bits (798), Expect = 4.6e-85
Identity = 155/333 (46.55%), Postives = 212/333 (63.66%), Query Frame = 1

Query: 9   LLITTLLLLFCFNVSKALDCELNYNDPHKVAYTIVVDKSGRGNFTTIQSAIDSIPLQNTK 68
           ++   ++ LF +  S A +         ++AYTI VD +G GNFTT+QSAIDSI   N  
Sbjct: 11  IVCMVVMTLFVYGYSAAAE---------QIAYTITVDLNGGGNFTTVQSAIDSISPPNHN 70

Query: 69  WIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQ 128
           WIRV    GIY EKV IP EK  I+L G G   T I+++DH  T  SATFT+ A+++V+ 
Sbjct: 71  WIRVFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVIS 130

Query: 129 GITFRNTHNAAGNVRRREDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCY 188
           GITF+NT+N   N +R  +I PA+AAR+ GD+ +     F+GLQDTL+DG GRH + RC 
Sbjct: 131 GITFKNTYNIVPNNKR--EIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCI 190

Query: 189 IEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMG 248
           I G ID I G+ QS++++C +N+ + +YAP    G ITA  +   ++  GFVF  CTV G
Sbjct: 191 ISGGIDFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTG 250

Query: 249 SGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSK 308
            G   LGRA+   + VIF  S L   + P GW++W   G+E DLT+ E+ C G GADTS+
Sbjct: 251 VGKTLLGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQ 310

Query: 309 RVPWLKRLVGVEIKHFTHISYIDQEGWTSRLPV 342
           RVPWLK+L   E+  F  +S+IDQ+GW SR P+
Sbjct: 311 RVPWLKKLSLSEVDGFASVSFIDQDGWISRFPI 332

BLAST of Lsi03G011210 vs. TAIR10
Match: AT2G19150.1 (AT2G19150.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 299.3 bits (765), Expect = 3.1e-81
Identity = 146/305 (47.87%), Postives = 194/305 (63.61%), Query Frame = 1

Query: 37  KVAYTIVVDKSGRGNFTTIQSAIDSIPLQNTKWIRVQIFPGIYSEKVMIPPEKPCIFLDG 96
           ++A TI+V+ +    F T+QSAIDSIPLQN  WIR+ I  GIYSEKV IP  K  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 97  GGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVRRREDIAPAIAARI 156
           GG   T I + DH  T TSATFTS   N+++ GITF+N +N A +        PA+AA +
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 157 EGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVINIPINVY 216
            GDK       F G QDTL+D +GRH + RC I G ID I G AQSI++ C + + + +Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 217 APILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCIN 276
            P    G ITAQG++   +  GFVF  CTVMGSG   LGRA++ +S VIF+ S     I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 277 PAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIKHFTHISYIDQEGWT 336
           P GW++W+  G E  +T+ E  C G GADTSKRVPWL +    ++  FT++++ID+EGW 
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWL 335

Query: 337 SRLPV 342
           SRLP+
Sbjct: 336 SRLPI 337

BLAST of Lsi03G011210 vs. TAIR10
Match: AT3G24130.1 (AT3G24130.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 297.0 bits (759), Expect = 1.5e-80
Identity = 139/301 (46.18%), Postives = 194/301 (64.45%), Query Frame = 1

Query: 42  IVVDKSGRGNFTTIQSAIDSIPLQNTKWIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRA 101
           + VD+SG GNFTTIQ AIDS+P+ N  W  + +  G+Y EK+ IP EKP I L G G+R 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 102 TEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVRRREDI-APAIAARIEGDK 161
           T ++W+DH + A S TF++ A+N VV+ ITF N++N     +  ++   PA+AA I GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 162 GIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVINIPINVYAPIL 221
             F+  GF G+QDTLWD  GRH F RC I+G +D I G  QSIYQ CVI +      P L
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPGL 213

Query: 222 NLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGW 281
             G+ITAQG+ +  +++GF+FI C V G+G  FLGR +R +S VIF+ S L   + P GW
Sbjct: 214 -AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGW 273

Query: 282 NSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIKHFTHISYIDQEGWTSRLP 341
           ++W  +GHE  L +AE  C G GA+  +RV W+K+L    I++   +S+I++ GW   LP
Sbjct: 274 DAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLP 333

BLAST of Lsi03G011210 vs. TAIR10
Match: AT5G26810.1 (AT5G26810.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 237.3 bits (604), Expect = 1.4e-62
Identity = 115/252 (45.63%), Postives = 158/252 (62.70%), Query Frame = 1

Query: 96  GGGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVRRRE----DIAPA 155
           G G+R T I +N H  T  S+TFTS   ++VV+ ++  NT+N   ++ +      DI PA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 156 IAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVINI 215
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G  QS+Y+ C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 216 PINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFL 275
                A  ++ G+ITAQG++  ++ SGFVF+  +V GS +V+LGRAY PFS VIF  + L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 276 PSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIK-HFTHISYI 335
            S ++P GW SW   G+EM  TYAE  C G G+D S+RVPW+ +L     K  F+  ++I
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 336 DQEGWTSRLPVF 343
           DQ+ W S +P F
Sbjct: 282 DQDQWISNIPRF 293

BLAST of Lsi03G011210 vs. NCBI nr
Match: gi|700203001|gb|KGN58134.1| (hypothetical protein Csa_3G536660 [Cucumis sativus])

HSP 1 Score: 557.8 bits (1436), Expect = 1.4e-155
Identity = 269/348 (77.30%), Postives = 299/348 (85.92%), Query Frame = 1

Query: 1   MEH--SKEIIL-LITTLLLLFCFNVSKALD-CELNYNDPHKVAYTIVVDKSGRGNFTTIQ 60
           MEH  SKEI L +ITTL++LFC  VSKALD CELNYNDPHKVAYTIVVDK G+GNFTTIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAIDSIPLQNTKWIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSA 120
           SAI+S+P Q+TKWIR+QI PGIY+EKV IP EK CIFLDG G + TEI WNDH TTA S 
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTSSAENLVVQGITFRNTHNAAGNVRRREDIAPAIAARIEGDKGIFHKCGFIGLQDTLW 180
           TFT+SA+NLVVQGITFRNT+NA G+V RREDI PA+AA I+GDK IFHKCGFIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGHGRHLFTRCYIEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANES 240
           DG GRHLFT+CYIEGVIDVISGF QSIY++CVINIP+N YAP+LN GFITAQGK + NES
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAE 300
           SGFVF+ C V GSGNVFLGRAYRPFSTVIFHL FLPSCINPAGWNSW Q GHE DLTY+E
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 SRCIGPGADTSKRVPWLKRLVGVEIKHFTHISYIDQEG-WTSRLPVFS 344
           +RCIGPGADTS RVPW+ RL    I+ FT ISYID +G WTSR+P+ S
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS 348

BLAST of Lsi03G011210 vs. NCBI nr
Match: gi|659093698|ref|XP_008447669.1| (PREDICTED: putative pectinesterase 52 [Cucumis melo])

HSP 1 Score: 442.6 bits (1137), Expect = 6.4e-121
Identity = 208/261 (79.69%), Postives = 228/261 (87.36%), Query Frame = 1

Query: 84  MIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAGNVR 143
           MIPP+K CIFLDG G R TEIQWNDH TTATS TFTS A+NLVVQGITFRNT+NA G+V 
Sbjct: 1   MIPPDKSCIFLDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVT 60

Query: 144 RREDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFAQSI 203
           RREDI PAIAA I+GDK IFHKCGFIGLQDTLWDG GRHLFT+CYIEGVIDVISGF QSI
Sbjct: 61  RREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSI 120

Query: 204 YQKCVINIPINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRPFST 263
           Y++CVINIPIN YAP+LN GFITAQGK + +ESSGFVFI C V GSG VFLGRAYRPFST
Sbjct: 121 YKECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFST 180

Query: 264 VIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVEIKH 323
           VIFHL FLPSCI+PAGWNSW+Q+GHE +LTY+E+RCIGPGADTS RVPWLKRL   +I+ 
Sbjct: 181 VIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRR 240

Query: 324 FTHISYIDQEG-WTSRLPVFS 344
           FT ISYID +G WTSRLPV S
Sbjct: 241 FTDISYIDPQGYWTSRLPVLS 261

BLAST of Lsi03G011210 vs. NCBI nr
Match: gi|778688667|ref|XP_011652807.1| (PREDICTED: probable pectinesterase 55 [Cucumis sativus])

HSP 1 Score: 437.6 bits (1124), Expect = 2.0e-119
Identity = 204/264 (77.27%), Postives = 225/264 (85.23%), Query Frame = 1

Query: 81  EKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQGITFRNTHNAAG 140
           EKV IP EK CIFLDG G + TEI WNDH TTA S TFT+SA+NLVVQGITFRNT+NA G
Sbjct: 33  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 92

Query: 141 NVRRREDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTRCYIEGVIDVISGFA 200
           +V RREDI PA+AA I+GDK IFHKCGFIGLQDTLWDG GRHLFT+CYIEGVIDVISGF 
Sbjct: 93  SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 152

Query: 201 QSIYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFVFIGCTVMGSGNVFLGRAYRP 260
           QSIY++CVINIP+N YAP+LN GFITAQGK + NESSGFVF+ C V GSGNVFLGRAYRP
Sbjct: 153 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 212

Query: 261 FSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADTSKRVPWLKRLVGVE 320
           FSTVIFHL FLPSCINPAGWNSW Q GHE DLTY+E+RCIGPGADTS RVPW+ RL    
Sbjct: 213 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH 272

Query: 321 IKHFTHISYIDQEG-WTSRLPVFS 344
           I+ FT ISYID +G WTSR+P+ S
Sbjct: 273 IRSFTDISYIDPQGYWTSRIPLLS 296

BLAST of Lsi03G011210 vs. NCBI nr
Match: gi|985469142|ref|XP_015380662.1| (PREDICTED: probable pectinesterase 55 [Citrus sinensis])

HSP 1 Score: 381.3 bits (978), Expect = 1.7e-102
Identity = 182/334 (54.49%), Postives = 226/334 (67.66%), Query Frame = 1

Query: 10  LITTLLLLFCFNVSKALDCELNYNDPHKVAYTIVVDKSGRGNFTTIQSAIDSIPLQNTKW 69
           L    LLL   NVS ALDC+LN  +P KVAYTI V +SG GNFT IQ AI+ IP+ N +W
Sbjct: 8   LFAVTLLLLSSNVSTALDCKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPVNNDQW 67

Query: 70  IRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTSSAENLVVQG 129
           IR+QI P IY EKV IP  KPCIFL+G   R T+IQW DH TT +S TFTS +EN+V +G
Sbjct: 68  IRIQISPEIYQEKVTIPQNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSENVVAKG 127

Query: 130 ITFRNTHNAAGNVRR---REDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHGRHLFTR 189
           I F+NT+N   + R+   R ++  A+AARI GDK  F+ CGF GLQDTL+D HGRH F +
Sbjct: 128 ILFQNTYNVPPSYRQNQERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQ 187

Query: 190 CYIEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFVFIGCTV 249
           CYIEG ID I G  QSIY  C +N+ +  Y P  + G+ITAQG+N  ++ SGFVF  C  
Sbjct: 188 CYIEGAIDFIFGNGQSIYHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVFTSCQF 247

Query: 250 MGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCIGPGADT 309
           +GSG  +LGRAY  FS VI   S L   + P GWN+W  + HE ++ YAE+RC G GADT
Sbjct: 248 LGSGKAYLGRAYGAFSRVIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRGAGADT 307

Query: 310 SKRVPWLKRLVGVEIKHFTHISYIDQEGWTSRLP 341
           SKRV W K L   E+K FT  S+ID +GW ++LP
Sbjct: 308 SKRVSWEKHLSVDEVKKFTDSSFIDHDGWIAKLP 341

BLAST of Lsi03G011210 vs. NCBI nr
Match: gi|590719762|ref|XP_007051145.1| (Pectinesterase, putative [Theobroma cacao])

HSP 1 Score: 376.3 bits (965), Expect = 5.6e-101
Identity = 178/343 (51.90%), Postives = 234/343 (68.22%), Query Frame = 1

Query: 1   MEHSKEIILLITTLLLLFCFNVSKALDCELNYNDPHKVAYTIVVDKSGRGNFTTIQSAID 60
           M+  + I+ LI  +LL F  ++SKAL+C+LN  +  KVA+TI VD+SG+G+F TIQ+AID
Sbjct: 17  MQFLQHILALIFIVLLYF--SISKALECQLNNANKFKVAHTIRVDQSGKGDFKTIQNAID 76

Query: 61  SIPLQNTKWIRVQIFPGIYSEKVMIPPEKPCIFLDGGGRRATEIQWNDHTTTATSATFTS 120
           SIP  N KWIR+ I PG + EKV IP  KPCIFL+G G + T I+W DH  T  SATFTS
Sbjct: 77  SIPSNNDKWIRILISPGHFREKVTIPANKPCIFLEGAGSKLTSIEWGDHMDTCDSATFTS 136

Query: 121 SAENLVVQGITFRNTHNAAGNVRRREDIAPAIAARIEGDKGIFHKCGFIGLQDTLWDGHG 180
           S +N+V +GI+F+N +N       ++ + PA+A RI GDK  F+ C F GLQDTLWD  G
Sbjct: 137 SPDNIVAKGISFKNIYNIPSGPDAKKKVEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQG 196

Query: 181 RHLFTRCYIEGVIDVISGFAQSIYQKCVINIPINVYAPILNLGFITAQGKNDANESSGFV 240
           RH F  CYIEG +D I G  QSIY+KC IN+    YAP    G+ITAQG+N +++ SGFV
Sbjct: 197 RHYFYNCYIEGAVDFIFGSGQSIYEKCEINLTAGKYAPEYPNGYITAQGRNSSDDPSGFV 256

Query: 241 FIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCINPAGWNSWEQLGHEMDLTYAESRCI 300
           F  C   G G  +LGRAY  +S VI + S L   I  +GW++W  + HE +L Y E+ C 
Sbjct: 257 FKYCVFTGYGKTYLGRAYGAYSRVIIYKSVLSDAILASGWDAWRYVHHEGNLIYVEADCK 316

Query: 301 GPGADTSKRVPWLKRLVGVEIKHFTHISYIDQEGWTSRLPVFS 344
           GPGA+TSKRVPWLK+L   ++K F ++SYID+EGW ++LP  S
Sbjct: 317 GPGANTSKRVPWLKKLGASQLKKFLNLSYIDKEGWIAKLPKVS 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME55_ARATH2.5e-8546.62Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1[more]
PME66_ARATH8.1e-8446.55Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2[more]
PME10_ARATH5.4e-8047.87Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1[more]
PME29_ARATH2.7e-7946.18Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1[more]
PME52_ARATH2.5e-6145.63Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LB12_CUCSA9.5e-15677.30Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1[more]
A0A067HG98_CITSI1.2e-10254.49Pectinesterase OS=Citrus sinensis GN=CISIN_1g043974mg PE=4 SV=1[more]
A0A061DSC1_THECC3.9e-10151.90Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1[more]
V4VWS4_9ROSI9.6e-10054.72Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1[more]
A0A0D2U2R3_GOSRA1.7e-9650.74Pectinesterase OS=Gossypium raimondii GN=B456_008G138100 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G18990.11.4e-8646.62 Pectin lyase-like superfamily protein[more]
AT2G47280.14.6e-8546.55 Pectin lyase-like superfamily protein[more]
AT2G19150.13.1e-8147.87 Pectin lyase-like superfamily protein[more]
AT3G24130.11.5e-8046.18 Pectin lyase-like superfamily protein[more]
AT5G26810.11.4e-6245.63 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|700203001|gb|KGN58134.1|1.4e-15577.30hypothetical protein Csa_3G536660 [Cucumis sativus][more]
gi|659093698|ref|XP_008447669.1|6.4e-12179.69PREDICTED: putative pectinesterase 52 [Cucumis melo][more]
gi|778688667|ref|XP_011652807.1|2.0e-11977.27PREDICTED: probable pectinesterase 55 [Cucumis sativus][more]
gi|985469142|ref|XP_015380662.1|1.7e-10254.49PREDICTED: probable pectinesterase 55 [Citrus sinensis][more]
gi|590719762|ref|XP_007051145.1|5.6e-10151.90Pectinesterase, putative [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G011210.1Lsi03G011210.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 42..331
score: 5.6
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 38..338
score: 8.98
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 38..337
score: 1.8
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 37..342
score: 4.2E
NoneNo IPR availablePANTHERPTHR31321:SF32PECTINESTERASE 52-RELATEDcoord: 37..342
score: 4.2E

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi03G011210Bottle gourd (USVL1VR-Ls)lsilsiB091
Lsi03G011210Cucumber (Gy14) v1cgylsiB352
Lsi03G011210Cucurbita maxima (Rimu)cmalsiB240
Lsi03G011210Cucurbita maxima (Rimu)cmalsiB350
Lsi03G011210Cucurbita maxima (Rimu)cmalsiB351
Lsi03G011210Cucurbita maxima (Rimu)cmalsiB831
Lsi03G011210Cucurbita maxima (Rimu)cmalsiB832
Lsi03G011210Cucurbita moschata (Rifu)cmolsiB228
Lsi03G011210Cucurbita moschata (Rifu)cmolsiB339
Lsi03G011210Cucurbita moschata (Rifu)cmolsiB340
Lsi03G011210Cucurbita moschata (Rifu)cmolsiB817
Lsi03G011210Cucurbita moschata (Rifu)cmolsiB818
Lsi03G011210Cucurbita pepo (Zucchini)cpelsiB115
Lsi03G011210Cucurbita pepo (Zucchini)cpelsiB116
Lsi03G011210Cucurbita pepo (Zucchini)cpelsiB266
Lsi03G011210Cucurbita pepo (Zucchini)cpelsiB500
Lsi03G011210Wild cucumber (PI 183967)cpilsiB530
Lsi03G011210Cucumber (Chinese Long) v2culsiB516
Lsi03G011210Melon (DHL92) v3.5.1lsimeB240
Lsi03G011210Melon (DHL92) v3.5.1lsimeB252
Lsi03G011210Watermelon (Charleston Gray)lsiwcgB217
Lsi03G011210Watermelon (Charleston Gray)lsiwcgB246
Lsi03G011210Watermelon (97103) v1lsiwmB234
Lsi03G011210Watermelon (97103) v1lsiwmB280
Lsi03G011210Cucumber (Gy14) v2cgyblsiB483
Lsi03G011210Melon (DHL92) v3.6.1lsimedB266
Lsi03G011210Silver-seed gourdcarlsiB064
Lsi03G011210Silver-seed gourdcarlsiB099
Lsi03G011210Silver-seed gourdcarlsiB410
Lsi03G011210Silver-seed gourdcarlsiB699
Lsi03G011210Silver-seed gourdcarlsiB748
Lsi03G011210Cucumber (Chinese Long) v3cuclsiB550
Lsi03G011210Watermelon (97103) v2lsiwmbB239
Lsi03G011210Watermelon (97103) v2lsiwmbB253
Lsi03G011210Wax gourdlsiwgoB343