Csa3G536660 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa3G536660
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Pectinesterase; contains IPR011050 (Pectin lyase fold/virulence factor)
Location	Chr3 : 21766526 .. 21768479 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACATTATTATTCGAAAGAGATAAGTTTGTTCATTATAACGACATTGGTGGTGTTGTTTTGTGTGAAAGTTTCCAAGGCATTGGATGATTGTGAATTGAATTATAATGATCCTCATAAAGTTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCACCACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAATTTCTCCTGGCATATATACGTACGGTATACGATCTTATTTCCCGTTTATTAAAATATCATTTTAGTTCCGTTGTTTGATTTCATTTTATTTTCATCAAGTTTAGATTTAGTTTTTCTTTTTCTTTTTTCCATACCTACTAAAGTCTATCACTGTTTGATTTCTATCAGCATTTATTAGTGATAGGTGATAGTTATAGAAACATGAATAGTCTAATTAGACATGCATAGAACCTTTATATTGAGGTATATACTAAAAGAGGCACTACGTGACAATAATAACTTTTGAGCATGTGATACATCAAGCAAAGTTTTTTAAGCATATAAATGTATCAATTCATATGTTATGAAGTTTCTTTTACTATAAGAATGATATTATATTTTTAAGAAAGAATATTTTAATAAATGTGTAATCATCATGTCGTGTCTTAGATTTTTAAATAATGGCGTGCTGTGGTGTCCCTATTGTGCCATATCCATGTCTCAAATTTGAATTTGTGCTATTAGGATCGGATGACAGTAGTCTTGTTTATCGTCTATCAATGATATTTTGTTATATATACTTGGAATCAATTAAAACAATCATTACACATGTTATATTTTAACAGGGAAAAAGTGACGATACCCTCAGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGGTATATACATATAACTGTTTAATTTCCCTAACTTTCAAATAATGTATATATTTTCTTTGTTTTATGTCTATTATCTGTGATCCAAAATTGGCAGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCCGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGTTAACTTCTAAATTAATTATAAGCTTACATGTGTGTTTGTATATGTGTGAGAGCTTGGAAGTGAAATTGAAGTGTTGAGTTTTTCTTTTCTGTTTGTTTGTTTGTTGTGTTTTAACGCAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGTAATAATTAACTCTTCTTTTCATCTTTTTCATTTCATTTTATATAGTATATTATTAAAGTATCATTATTATCATTATTATGATTATTATCATCATTTGTTTGTAGGAGTGATTTGACGTATTCAGAGACCCGTTGTATTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGATTCCTCTTTTATCCTAA

mRNA sequence

ATGGAACATTATTATTCGAAAGAGATAAGTTTGTTCATTATAACGACATTGGTGGTGTTGTTTTGTGTGAAAGTTTCCAAGGCATTGGATGATTGTGAATTGAATTATAATGATCCTCATAAAGTTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCACCACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAATTTCTCCTGGCATATATACGGAAAAAGTGACGATACCCTCAGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCCGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGAGTGATTTGACGTATTCAGAGACCCGTTGTATTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGATTCCTCTTTTATCCTAA

Coding sequence (CDS)

ATGGAACATTATTATTCGAAAGAGATAAGTTTGTTCATTATAACGACATTGGTGGTGTTGTTTTGTGTGAAAGTTTCCAAGGCATTGGATGATTGTGAATTGAATTATAATGATCCTCATAAAGTTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCACCACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAATTTCTCCTGGCATATATACGGAAAAAGTGACGATACCCTCAGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCCGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGAGTGATTTGACGTATTCAGAGACCCGTTGTATTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGATTCCTCTTTTATCCTAA

Protein sequence

MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS*

Homology

[Close]

BLAST of Csa3G536660 vs. Swiss-Prot
Match: PME66_ARATH (Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2)

HSP 1 Score: 288.5 bits (737), Expect = 9.8e-77
Identity = 143/306 (46.73%), Postives = 192/306 (62.75%), Query Frame = 1


Query: 41  KVAYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDG 100
           ++AYTI VD  G GNFTT+QSAI+S+   +  WIR+    GIY EKVTIP EK  I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I ++DH+ T  S TFTA A ++V+ GITF+NTYN   +   + +I PA+AA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GD+ +     F+GLQDTL+DG GRH + +C I G ID I G+GQS++KEC +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
           AP    G ITA  + +P++  GFVF  C V G G   LGRA+   + VIF    L   + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 281 PAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYW 340
           P GW++W   G+E DLT+ E  C G GADTS RVPW+ +L    +  F  +S+ID  G W
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDG-W 329

Query: 341 TSRIPL 347
            SR P+
Sbjct: 330 ISRFPI 332

[Close]

BLAST of Csa3G536660 vs. Swiss-Prot
Match: PME55_ARATH (Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-76
Identity = 141/313 (45.05%), Postives = 197/313 (62.94%), Query Frame = 1


Query: 39  PHKVA---YTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSC 98
           PH +    + ++VD+ G GNFTTIQ AI+SVP  +T W  I +  G+Y EK+TIP +K  
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 99  IFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIK 158
           I + G+G + T + W+DH + A SPTF   A N VV+ ITF N+YN  + G + +   + 
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV- 138

Query: 159 PALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVI 218
           PA+AA I GDK  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI
Sbjct: 139 PAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVI 198

Query: 219 NIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLC 278
            +      P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LGRA+RP+S VIF+  
Sbjct: 199 QVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNS 258

Query: 279 FLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISY 338
            L   ++P GW  W   G+E  LTY+E  C G G++TS R  WV +L A  ++   D+S+
Sbjct: 259 NLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSF 318

Query: 339 IDPQGYWTSRIPL 347
           I+ +G W   +P+
Sbjct: 319 IN-RGGWVEDLPI 328

[Close]

BLAST of Csa3G536660 vs. Swiss-Prot
Match: PME29_ARATH (Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 7.7e-74
Identity = 139/303 (45.87%), Postives = 192/303 (63.37%), Query Frame = 1


Query: 46  IVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQV 105
           + VD+ G GNFTTIQ AI+SVP  +  W  I +  G+Y EK+ IP EK  I L G+G ++
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 106 TEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQGD 165
           T + W+DH + A SPTF+  A N VV+ ITF N+YN  ++G  M +    PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGK-MNKNPRTPAVAALIGGD 153

Query: 166 KVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPL 225
           K  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      P 
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPG 213

Query: 226 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 285
           L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLGR +R +S VIF+   L   + P G
Sbjct: 214 L-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEG 273

Query: 286 WNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSR 345
           W++W   GHE+ L ++E  C G GA+   RV WV +L    I++  D+S+I+ +G W   
Sbjct: 274 WDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFIN-RGGWVED 333

Query: 346 IPL 347
           +P+
Sbjct: 334 LPI 333

[Close]

BLAST of Csa3G536660 vs. Swiss-Prot
Match: PME10_ARATH (Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.0e-70
Identity = 135/306 (44.12%), Postives = 185/306 (60.46%), Query Frame = 1


Query: 41  KVAYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDG 100
           ++A TI+V+      F T+QSAI+S+P Q+  WIRI IS GIY+EKVTIP  K  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I + DH+ T  S TFT+   N+++ GITF+N YN   S       KPA+AA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GDK       F G QDTL+D  GRH + +C I G ID I G  QSI++ C + + V  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
            P    G ITAQG+++P +  GFVF  C V GSG   LGRA++ +S VIF+       I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 281 PAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYW 340
           P GW++W   G E  +T+ E  C G GADTS RVPW+ +     +  FT++++ID +G W
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEG-W 335

Query: 341 TSRIPL 347
            SR+P+
Sbjct: 336 LSRLPI 337

[Close]

BLAST of Csa3G536660 vs. Swiss-Prot
Match: PME52_ARATH (Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 7.5e-61
Identity = 118/250 (47.20%), Postives = 154/250 (61.60%), Query Frame = 1


Query: 100 GSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRRE----DIKPA 159
           G G +VT I +N H  T  S TFT+   ++VV+ ++  NTYN   S+ +      DIKPA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 160 LAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 219
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G GQS+Y++C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 220 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFL 279
              A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LGRAY PFS VIF    L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 280 PSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYID 339
            S ++P GW SW   G+E   TY+E  C G G+D S RVPW+++L +F+ +    IS   
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 340 PQGYWTSRIP 346
            Q  W S IP
Sbjct: 282 DQDQWISNIP 291

[Close]

BLAST of Csa3G536660 vs. TrEMBL
Match: A0A0A0LB12_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 1.9e-204
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 1


Query: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60
           MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120
           SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180
           TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240
           DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300
           SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS 349
           TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS 348

[Close]

BLAST of Csa3G536660 vs. TrEMBL
Match: A0A067HG98_CITSI (Pectinesterase OS=Citrus sinensis GN=CISIN_1g043974mg PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.7e-91
Identity = 172/340 (50.59%), Postives = 221/340 (65.00%), Query Frame = 1


Query: 9   ISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQSAINSVPS 68
           ++ F+    ++L    VS ALD C+LN  +P KVAYTI V + G GNFT IQ AIN +P 
Sbjct: 4   LNSFLFAVTLLLLSSNVSTALD-CKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPV 63

Query: 69  QSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQN 128
            + +WIRIQISP IY EKVTIP  K CIFL+G+  + T+I W DH+TT +SPTFT+ ++N
Sbjct: 64  NNDQWIRIQISPEIYQEKVTIPQNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSEN 123

Query: 129 LVVQGITFRNTYNARGSVMR---REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGR 188
           +V +GI F+NTYN   S  +   R ++K ALAA I GDK  F+ CGF GLQDTL+D  GR
Sbjct: 124 VVAKGILFQNTYNVPPSYRQNQERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGR 183

Query: 189 HLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVF 248
           H F QCYIEG ID I G GQSIY  C +N+ +  Y P  + G+ITAQG+ + ++ SGFVF
Sbjct: 184 HYFHQCYIEGAIDFIFGNGQSIYHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVF 243

Query: 249 LRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIG 308
             C   GSG  +LGRAY  FS VI     L   + P GWN+W    HE ++ Y+E RC G
Sbjct: 244 TSCQFLGSGKAYLGRAYGAFSRVIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRG 303

Query: 309 PGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIP 346
            GADTS RV W   L    ++ FTD S+ID  G W +++P
Sbjct: 304 AGADTSKRVSWEKHLSVDEVKKFTDSSFIDHDG-WIAKLP 341

[Close]

BLAST of Csa3G536660 vs. TrEMBL
Match: A0A061DSC1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.1e-90
Identity = 165/331 (49.85%), Postives = 222/331 (67.07%), Query Frame = 1


Query: 18  VVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQ 77
           +VL    +SKAL+ C+LN  +  KVA+TI VD+ GKG+F TIQ+AI+S+PS + KWIRI 
Sbjct: 29  IVLLYFSISKALE-CQLNNANKFKVAHTIRVDQSGKGDFKTIQNAIDSIPSNNDKWIRIL 88

Query: 78  ISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFR 137
           ISPG + EKVTIP+ K CIFL+G+G ++T I W DH  T  S TFT+S  N+V +GI+F+
Sbjct: 89  ISPGHFREKVTIPANKPCIFLEGAGSKLTSIEWGDHMDTCDSATFTSSPDNIVAKGISFK 148

Query: 138 NTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 197
           N YN       ++ ++PALA  I GDK  F+ C F GLQDTLWD  GRH F  CYIEG +
Sbjct: 149 NIYNIPSGPDAKKKVEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQGRHYFYNCYIEGAV 208

Query: 198 DVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVF 257
           D I G GQSIY++C IN+    YAP    G+ITAQG+ + ++ SGFVF  C+  G G  +
Sbjct: 209 DFIFGSGQSIYEKCEINLTAGKYAPEYPNGYITAQGRNSSDDPSGFVFKYCVFTGYGKTY 268

Query: 258 LGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWV 317
           LGRAY  +S VI +   L   I  +GW++W    HE +L Y E  C GPGA+TS RVPW+
Sbjct: 269 LGRAYGAYSRVIIYKSVLSDAILASGWDAWRYVHHEGNLIYVEADCKGPGANTSKRVPWL 328

Query: 318 NRLDAFHIRSFTDISYIDPQGYWTSRIPLLS 349
            +L A  ++ F ++SYID +G W +++P +S
Sbjct: 329 KKLGASQLKKFLNLSYIDKEG-WIAKLPKVS 357

[Close]

BLAST of Csa3G536660 vs. TrEMBL
Match: V4VWS4_9ROSI (Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 7.0e-90
Identity = 166/318 (52.20%), Postives = 210/318 (66.04%), Query Frame = 1


Query: 31  DCELNYNDPHKVAYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIP 90
           DC+LN  +P KVAYTI V + G GNFT IQ AIN +P  + +WIRIQISP IY EKVTIP
Sbjct: 2   DCKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPVNNDQWIRIQISPEIYQEKVTIP 61

Query: 91  SEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR-- 150
             K CIFL+G+  + T+I W DH+TT +SPTFT+ ++N+V +GI F+NTYN   S  +  
Sbjct: 62  QNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSENVVAKGILFQNTYNVPPSYRQNQ 121

Query: 151 -REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSI 210
            R ++K ALAA I GDK  F+ CGF GLQDTL+D  GRH F QCYIEG ID I G GQSI
Sbjct: 122 ERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQCYIEGAIDFIFGNGQSI 181

Query: 211 YKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFST 270
           Y  C +N+ +  Y P  + G+ITAQG+ + ++ SGFVF  C   GSG  +LGRAY  FS 
Sbjct: 182 YHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVFTSCQFLGSGKAYLGRAYGAFSR 241

Query: 271 VIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRS 330
           VI     L   + P GWN+W    HE ++ Y+E RC G GADTS RV W   L    ++ 
Sbjct: 242 VIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRGAGADTSKRVSWEKHLSVDEVKK 301

Query: 331 FTDISYIDPQGYWTSRIP 346
           FTD S+ID  G W +++P
Sbjct: 302 FTDSSFIDHDG-WIAKLP 318

[Close]

BLAST of Csa3G536660 vs. TrEMBL
Match: A0A067K902_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_14050 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.2e-86
Identity = 160/323 (49.54%), Postives = 217/323 (67.18%), Query Frame = 1


Query: 18  VVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQ 77
           +++ C  V KALD C+LN ++  KVAYTI VDK GKGNFTTIQSAI+SVP  +T WIRIQ
Sbjct: 10  ILVLCSSVCKALD-CQLNKSNSLKVAYTISVDKSGKGNFTTIQSAIDSVPDGNTHWIRIQ 69

Query: 78  ISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFR 137
           ISP  Y EK+TIP +K CIFL+G+  ++T I W+DH  T  S TF++ ++NL+ +GI F+
Sbjct: 70  ISPNTYREKITIPMKKPCIFLEGADKELTSIEWDDHGVTGTSATFSSYSENLIAKGIKFK 129

Query: 138 NTYNARGSVMRRE-DIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGV 197
           NTYN    V       + A++A I+GDK  F+ C F+G+QDT WD  GRH F +CY EG 
Sbjct: 130 NTYNWNLPVKAENFSWRQAVSARIRGDKSAFYDCAFVGIQDTFWDEKGRHYFYKCYFEGA 189

Query: 198 IDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNV 257
           +D I G  +SIY++C++++    Y P +  G+ITAQ KE     SGFVF  C + GSG  
Sbjct: 190 VDFIFGKAKSIYEKCLLSVNKGRYDPEI-PGYITAQKKEWAEHESGFVFKNCEINGSGKA 249

Query: 258 FLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPW 317
           +LGRA+ PFS+V+ +   +   I P GWN+W   GHE++ TY E    GPGA+TS RVPW
Sbjct: 250 YLGRAWGPFSSVVIYNSTISEVIVPQGWNAWDYVGHEANFTYVEVDNRGPGAETSKRVPW 309

Query: 318 VNRLDAFHIRSFTDISYIDPQGY 340
           VN+L+A  +  +  +SYID  G+
Sbjct: 310 VNQLNASELSKYLTLSYIDSDGW 330

[Close]

BLAST of Csa3G536660 vs. TAIR10
Match: AT2G47280.1 (AT2G47280.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 288.5 bits (737), Expect = 5.5e-78
Identity = 143/306 (46.73%), Postives = 192/306 (62.75%), Query Frame = 1


Query: 41  KVAYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDG 100
           ++AYTI VD  G GNFTT+QSAI+S+   +  WIR+    GIY EKVTIP EK  I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I ++DH+ T  S TFTA A ++V+ GITF+NTYN   +   + +I PA+AA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GD+ +     F+GLQDTL+DG GRH + +C I G ID I G+GQS++KEC +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
           AP    G ITA  + +P++  GFVF  C V G G   LGRA+   + VIF    L   + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 281 PAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYW 340
           P GW++W   G+E DLT+ E  C G GADTS RVPW+ +L    +  F  +S+ID  G W
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDG-W 329

Query: 341 TSRIPL 347
            SR P+
Sbjct: 330 ISRFPI 332

[Close]

BLAST of Csa3G536660 vs. TAIR10
Match: AT5G18990.1 (AT5G18990.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 288.1 bits (736), Expect = 7.2e-78
Identity = 141/313 (45.05%), Postives = 197/313 (62.94%), Query Frame = 1


Query: 39  PHKVA---YTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSC 98
           PH +    + ++VD+ G GNFTTIQ AI+SVP  +T W  I +  G+Y EK+TIP +K  
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 99  IFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIK 158
           I + G+G + T + W+DH + A SPTF   A N VV+ ITF N+YN  + G + +   + 
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV- 138

Query: 159 PALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVI 218
           PA+AA I GDK  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI
Sbjct: 139 PAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVI 198

Query: 219 NIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLC 278
            +      P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LGRA+RP+S VIF+  
Sbjct: 199 QVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNS 258

Query: 279 FLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISY 338
            L   ++P GW  W   G+E  LTY+E  C G G++TS R  WV +L A  ++   D+S+
Sbjct: 259 NLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSF 318

Query: 339 IDPQGYWTSRIPL 347
           I+ +G W   +P+
Sbjct: 319 IN-RGGWVEDLPI 328

[Close]

BLAST of Csa3G536660 vs. TAIR10
Match: AT3G24130.1 (AT3G24130.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 278.9 bits (712), Expect = 4.4e-75
Identity = 139/303 (45.87%), Postives = 192/303 (63.37%), Query Frame = 1


Query: 46  IVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQV 105
           + VD+ G GNFTTIQ AI+SVP  +  W  I +  G+Y EK+ IP EK  I L G+G ++
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 106 TEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQGD 165
           T + W+DH + A SPTF+  A N VV+ ITF N+YN  ++G  M +    PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGK-MNKNPRTPAVAALIGGD 153

Query: 166 KVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPL 225
           K  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      P 
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPG 213

Query: 226 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 285
           L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLGR +R +S VIF+   L   + P G
Sbjct: 214 L-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEG 273

Query: 286 WNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSR 345
           W++W   GHE+ L ++E  C G GA+   RV WV +L    I++  D+S+I+ +G W   
Sbjct: 274 WDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFIN-RGGWVED 333

Query: 346 IPL 347
           +P+
Sbjct: 334 LPI 333

[Close]

BLAST of Csa3G536660 vs. TAIR10
Match: AT2G19150.1 (AT2G19150.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 266.9 bits (681), Expect = 1.7e-71
Identity = 135/306 (44.12%), Postives = 185/306 (60.46%), Query Frame = 1


Query: 41  KVAYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDG 100
           ++A TI+V+      F T+QSAI+S+P Q+  WIRI IS GIY+EKVTIP  K  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 101 SGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALI 160
            G++ T I + DH+ T  S TFT+   N+++ GITF+N YN   S       KPA+AA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 161 QGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAY 220
            GDK       F G QDTL+D  GRH + +C I G ID I G  QSI++ C + + V  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 221 APLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCIN 280
            P    G ITAQG+++P +  GFVF  C V GSG   LGRA++ +S VIF+       I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 281 PAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYW 340
           P GW++W   G E  +T+ E  C G GADTS RVPW+ +     +  FT++++ID +G W
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEG-W 335

Query: 341 TSRIPL 347
            SR+P+
Sbjct: 336 LSRLPI 337

[Close]

BLAST of Csa3G536660 vs. TAIR10
Match: AT5G26810.1 (AT5G26810.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 235.7 bits (600), Expect = 4.2e-62
Identity = 118/250 (47.20%), Postives = 154/250 (61.60%), Query Frame = 1


Query: 100 GSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRRE----DIKPA 159
           G G +VT I +N H  T  S TFT+   ++VV+ ++  NTYN   S+ +      DIKPA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 160 LAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 219
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G GQS+Y++C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 220 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFL 279
              A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LGRAY PFS VIF    L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 280 PSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYID 339
            S ++P GW SW   G+E   TY+E  C G G+D S RVPW+++L +F+ +    IS   
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 340 PQGYWTSRIP 346
            Q  W S IP
Sbjct: 282 DQDQWISNIP 291

[Close]

BLAST of Csa3G536660 vs. NCBI nr
Match: gi|700203001|gb|KGN58134.1| (hypothetical protein Csa_3G536660 [Cucumis sativus])

HSP 1 Score: 719.5 bits (1856), Expect = 2.7e-204
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 1


Query: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60
           MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120
           SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180
           TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240
           DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300
           SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS 349
           TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS 348

[Close]

BLAST of Csa3G536660 vs. NCBI nr
Match: gi|778688667|ref|XP_011652807.1| (PREDICTED: probable pectinesterase 55 [Cucumis sativus])

HSP 1 Score: 552.0 bits (1421), Expect = 7.6e-154
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 1


Query: 85  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 144
           EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG
Sbjct: 33  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 92

Query: 145 SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 204
           SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG
Sbjct: 93  SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 152

Query: 205 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 264
           QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP
Sbjct: 153 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 212

Query: 265 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH 324
           FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH
Sbjct: 213 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH 272

Query: 325 IRSFTDISYIDPQGYWTSRIPLLS 349
           IRSFTDISYIDPQGYWTSRIPLLS
Sbjct: 273 IRSFTDISYIDPQGYWTSRIPLLS 296

[Close]

BLAST of Csa3G536660 vs. NCBI nr
Match: gi|659093698|ref|XP_008447669.1| (PREDICTED: putative pectinesterase 52 [Cucumis melo])

HSP 1 Score: 493.0 bits (1268), Expect = 4.2e-136
Identity = 230/260 (88.46%), Postives = 244/260 (93.85%), Query Frame = 1


Query: 89  IPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR 148
           IP +KSCIFLDGSG +VTEI WNDH TTA SPTFT+ AQNLVVQGITFRNTYNA GSV R
Sbjct: 2   IPPDKSCIFLDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTR 61

Query: 149 REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY 208
           REDIKPA+AALIQGDK IFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY
Sbjct: 62  REDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY 121

Query: 209 KECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTV 268
           KECVINIP+NAYAPLLNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLGRAYRPFSTV
Sbjct: 122 KECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTV 181

Query: 269 IFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSF 328
           IFHLCFLPSCI+PAGWNSW Q GHES+LTYSETRCIGPGADTSSRVPW+ RLD + IR F
Sbjct: 182 IFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRF 241

Query: 329 TDISYIDPQGYWTSRIPLLS 349
           TDISYIDPQGYWTSR+P+LS
Sbjct: 242 TDISYIDPQGYWTSRLPVLS 261

[Close]

BLAST of Csa3G536660 vs. NCBI nr
Match: gi|985469142|ref|XP_015380662.1| (PREDICTED: probable pectinesterase 55 [Citrus sinensis])

HSP 1 Score: 344.4 bits (882), Expect = 2.4e-91
Identity = 172/340 (50.59%), Postives = 221/340 (65.00%), Query Frame = 1


Query: 9   ISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQSAINSVPS 68
           ++ F+    ++L    VS ALD C+LN  +P KVAYTI V + G GNFT IQ AIN +P 
Sbjct: 4   LNSFLFAVTLLLLSSNVSTALD-CKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPV 63

Query: 69  QSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQN 128
            + +WIRIQISP IY EKVTIP  K CIFL+G+  + T+I W DH+TT +SPTFT+ ++N
Sbjct: 64  NNDQWIRIQISPEIYQEKVTIPQNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSEN 123

Query: 129 LVVQGITFRNTYNARGSVMR---REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGR 188
           +V +GI F+NTYN   S  +   R ++K ALAA I GDK  F+ CGF GLQDTL+D  GR
Sbjct: 124 VVAKGILFQNTYNVPPSYRQNQERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGR 183

Query: 189 HLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVF 248
           H F QCYIEG ID I G GQSIY  C +N+ +  Y P  + G+ITAQG+ + ++ SGFVF
Sbjct: 184 HYFHQCYIEGAIDFIFGNGQSIYHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVF 243

Query: 249 LRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIG 308
             C   GSG  +LGRAY  FS VI     L   + P GWN+W    HE ++ Y+E RC G
Sbjct: 244 TSCQFLGSGKAYLGRAYGAFSRVIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRG 303

Query: 309 PGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIP 346
            GADTS RV W   L    ++ FTD S+ID  G W +++P
Sbjct: 304 AGADTSKRVSWEKHLSVDEVKKFTDSSFIDHDG-WIAKLP 341

[Close]

BLAST of Csa3G536660 vs. NCBI nr
Match: gi|590719762|ref|XP_007051145.1| (Pectinesterase, putative [Theobroma cacao])

HSP 1 Score: 341.7 bits (875), Expect = 1.6e-90
Identity = 165/331 (49.85%), Postives = 222/331 (67.07%), Query Frame = 1


Query: 18  VVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQ 77
           +VL    +SKAL+ C+LN  +  KVA+TI VD+ GKG+F TIQ+AI+S+PS + KWIRI 
Sbjct: 29  IVLLYFSISKALE-CQLNNANKFKVAHTIRVDQSGKGDFKTIQNAIDSIPSNNDKWIRIL 88

Query: 78  ISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFR 137
           ISPG + EKVTIP+ K CIFL+G+G ++T I W DH  T  S TFT+S  N+V +GI+F+
Sbjct: 89  ISPGHFREKVTIPANKPCIFLEGAGSKLTSIEWGDHMDTCDSATFTSSPDNIVAKGISFK 148

Query: 138 NTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 197
           N YN       ++ ++PALA  I GDK  F+ C F GLQDTLWD  GRH F  CYIEG +
Sbjct: 149 NIYNIPSGPDAKKKVEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQGRHYFYNCYIEGAV 208

Query: 198 DVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVF 257
           D I G GQSIY++C IN+    YAP    G+ITAQG+ + ++ SGFVF  C+  G G  +
Sbjct: 209 DFIFGSGQSIYEKCEINLTAGKYAPEYPNGYITAQGRNSSDDPSGFVFKYCVFTGYGKTY 268

Query: 258 LGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWV 317
           LGRAY  +S VI +   L   I  +GW++W    HE +L Y E  C GPGA+TS RVPW+
Sbjct: 269 LGRAYGAYSRVIIYKSVLSDAILASGWDAWRYVHHEGNLIYVEADCKGPGANTSKRVPWL 328

Query: 318 NRLDAFHIRSFTDISYIDPQGYWTSRIPLLS 349
            +L A  ++ F ++SYID +G W +++P +S
Sbjct: 329 KKLGASQLKKFLNLSYIDKEG-WIAKLPKVS 357

The following BLAST results are available for this feature:

BLAST of Csa3G536660 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PME66_ARATH	9.8e-77	46.73	Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2	[more]
PME55_ARATH	1.3e-76	45.05	Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1	[more]
PME29_ARATH	7.7e-74	45.87	Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1	[more]
PME10_ARATH	3.0e-70	44.12	Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1	[more]
PME52_ARATH	7.5e-61	47.20	Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2	[more]

BLAST of Csa3G536660 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB12_CUCSA	1.9e-204	100.00	Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1	[more]
A0A067HG98_CITSI	1.7e-91	50.59	Pectinesterase OS=Citrus sinensis GN=CISIN_1g043974mg PE=4 SV=1	[more]
A0A061DSC1_THECC	1.1e-90	49.85	Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1	[more]
V4VWS4_9ROSI	7.0e-90	52.20	Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1	[more]
A0A067K902_JATCU	1.2e-86	49.54	Pectinesterase OS=Jatropha curcas GN=JCGZ_14050 PE=4 SV=1	[more]

BLAST of Csa3G536660 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G47280.1	5.5e-78	46.73	Pectin lyase-like superfamily protein	[more]
AT5G18990.1	7.2e-78	45.05	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	4.4e-75	45.87	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	1.7e-71	44.12	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	4.2e-62	47.20	Pectin lyase-like superfamily protein	[more]

BLAST of Csa3G536660 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700203001\|gb\|KGN58134.1\|	2.7e-204	100.00	hypothetical protein Csa_3G536660 [Cucumis sativus]	[more]
gi\|778688667\|ref\|XP_011652807.1\|	7.6e-154	100.00	PREDICTED: probable pectinesterase 55 [Cucumis sativus]	[more]
gi\|659093698\|ref\|XP_008447669.1\|	4.2e-136	88.46	PREDICTED: putative pectinesterase 52 [Cucumis melo]	[more]
gi\|985469142\|ref\|XP_015380662.1\|	2.4e-91	50.59	PREDICTED: probable pectinesterase 55 [Citrus sinensis]	[more]
gi\|590719762\|ref\|XP_007051145.1\|	1.6e-90	49.85	Pectinesterase, putative [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000070	Pectinesterase_cat
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Cellular Component**
Term	Definition
GO:0005618	cell wall

Vocabulary: **Molecular Function**
Term	Definition
GO:0030599	pectinesterase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0042545	cell wall modification

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005618	cell wall
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU156914	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa3G536660.1	Csa3G536660.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU156914	CU156914	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 46..334 score: 3.4
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 42..340 score: 3.33
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 42..341 score: 7.0
None	No IPR available	PANTHER	PTHR31321	FAMILY NOT NAMED	coord: 41..347 score: 7.8E
None	No IPR available	PANTHER	PTHR31321:SF32	PECTINESTERASE 52-RELATED	coord: 41..347 score: 7.8E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa3G536660	Cla017349	Watermelon (97103) v1	cuwmB205
Csa3G536660	MELO3C012935	Melon (DHL92) v3.5.1	cumeB214
Csa3G536660	ClCG10G011540	Watermelon (Charleston Gray)	cuwcgB197
Csa3G536660	Lsi03G011210	Bottle gourd (USVL1VR-Ls)	culsiB190
Csa3G536660	MELO3C012935.2	Melon (DHL92) v3.6.1	cumedB197
Csa3G536660	Cla97C10G194370	Watermelon (97103) v2	cuwmbB189
Csa3G536660	Bhi11G001077	Wax gourd	cuwgoB247
Csa3G536660	CSPI03G25040	Wild cucumber (PI 183967)	cpicuB120
Csa3G536660	Cucsa.300590	Cucumber (Gy14) v1	cgycuB428
Csa3G536660	CmaCh01G002810	Cucurbita maxima (Rimu)	cmacuB451
Csa3G536660	CmoCh01G002890	Cucurbita moschata (Rifu)	cmocuB438
Csa3G536660	Cp4.1LG02g16160	Cucurbita pepo (Zucchini)	cpecuB563
Csa3G536660	CsGy3G023570	Cucumber (Gy14) v2	cgybcuB111

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None