Cp4.1LG02g16160 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG02g16160
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG02 : 13015590 .. 13021872 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTCTTATAATTTGTTTGTTCCAAGAAAAATGGAACATTACAATGACAAAATTTTGTTAATAACAACGTTGTGGTTGTTGTTACTTTGTCTCAATGTTTCAAACGCATTGGATTGTGAGTTGAATTTTAAAGATCCGAGTAGAGTTGCACGTACTATTTTTGTTCATCAGTTAGGGTCGGGAAATTTCAAAACAATCCAAAGTGCCATTGATTCGATCCCTCCACAAAATAATCGATGGATTAGAATTCTAATTTCTCCTGGCGTATATCGGTAAGATATCATTTTATTTATTTACCTTTCTTTGTTTATGTTGCGTGGTTAAAAAGTCTAATTTTTTTATTTAAACGTGTGAAATCTCACATCGGTCGGAGATGGGAACAAAACATTCCTTATAAGGGTGTGGAAACTTCTTCTTGATAGACGTGTTTTAAAATCGTGAAACTGATAGTGATACGTAATGGGCTAAAACGGACAATATTTACTAGTGGTAACAATATACCGTAAATTACAGGGAAAAGGTGAGAATTCCCCCGGAAAAACCATGCATCTTTCTAGATGGGGTTGATAGAAATCTCACTGAAATTCAATGGAATGACCATGAAACCACTGCTTCAAGTGCCACCTTCACTTTTTCTGCAGTAAATCTCATGGTACGAGGCATCTCGTTTAAGGTACGAGTTCTGACGAGAATTAATTCAAGTATTGAATTTAAATTTTGAATCTTGAACATGGAGCGTAAATGAAATTGACAGAATACTTATAATGCGCCGGGAAGTGTGACGTCACGTAATGATATAAAACCAGCACTTGCAGCTTTTGTAGACGGAGATAAAGCAGTCTTCCATCAGTGTGGCTTTTATGGTCTGCAAGATACTTTGTGGGACGGACCTGGTCGTCATCGGTACACCGAATGCTACATCGAAGGTGTAATCGATGTCATATCTGGTACTGGTCAATCTATATACGAGGTTAGATTTTATATTGTTTTAGCTATATATATATATATGGGAACTAAACATTCTTGATAAGAGTGTGAAAATCTCTCCCTAACCGACAGATTTTAAGACTGTGAGGCTAAGGTGATACATAACGGGCCAAAGTAAACAATATCTGTTTGTGGTGGGCTTTGACTGTTACAAATGGTATCAGACCTAGTCATCGGATGGTGTGCTAGTGAGGATGTTGGCCTCTAAGGGGTGGATTGTCAGATCCCACATGGGTTGGAGAGGAGAACGAAACATTCCTTGTAAAGGTGTGGAAATCTCCACTAGCAGATATGTGTTAAAACCATGAGACTGACAACGATACGTAACGAACCAAAGCGGACAATATCTGTTAGTGGTGATGTTTGGCTGTTACCACCTAGTCACTGGATGGTGTGCTTGTGAGGATGTTGGCCCCTAAGTGGTGGATTGTCATATCCCACATTAGTTGGAGAGGAGAACGAAACATTTTTTATAAAGGTGTGGAAACCTCCACTAGCAAACATGTTTTAAAACCATGAGTCTGACGACGATATGTAACGGGTCAAAGTGGACAACATCTGTTAGCAATGGGTTTTAGCTCTTACAAATGGTATCAAAGTCAGTCATCGGACCATGTGCCAGCGAGGACGCCGGACCTCTAAAATGGGTGGATTGTGAGATCTCACATTGGTTGTAGAGGGGAACGAAACGTTTCCTTATAAGGGTATAGAAACTTATCCCTAACAGACACGTTTTAAAATCGTGAGGCTGACGGCGATACGTAACGAGCCAAAACGGACAGTATCCGCTAGCGGTGGGCTTTGGCTATTACAAATGATGTCAGAGCCAATCACCGAACAGTGTGCTAGTGAGGACGCTAGACTTTCAATCATGGTGGACAGTGAGATCCCACATTAGTTGAAGAGGGGAACGAAACAGGGTATGAAAAAACTCTCTTAGCAGACGCATTTTAAAACCTTAAGAGGAACCTCGAAAGGTATCCACTAGCGACGATACGATATGATTCGAATCGGGTATACCCACGTTTAAAGATTGAATTGGTTCGGAGTTTTGATTTAGTTTTGTTTATGTGTTATTTACTGCAGAAGTGTGTGATAAATATACCAATCAACGTATATGCTCCAATATTAGGACATGGTTACATAACAGCTCAAGGAAAGGAAGCTTCAAGCCATACCAATGGGTTTGTATTCATTGAATGCTCAGTGATTGGAAGTGGAACTGTTTATCTTGGGAGGGCTTATAGACGTTTCTCCACAGTTATTTATCATAAATCATTCCTATCATCTTGTGTTAACCCTGTTGGTTGGCATCCTTGGACACAAGTTGGCCACGAGTATGTCTCCGCTTCTCCTTATAAATTTACTCGAACGAGTATCTAATGTTAGATGGATCGACATGCTCGAACAAGTACCTAATGCATCTAAATTATTTTGTTATATGATCTTGTAGGATGGATTTTACGTATGTGGAATCAGGTTGCACTGGACCAGGAGCAGACATTTCGAAGCGTGTGCCTTGGATTAAGAATCTTAATAAATATGATCTTAGACGTTTCATCGATATTTCTTTTATTGATCAAGATGGTTGGACTAGGAAGCTTCCTCAGTTTTAGAACTAAAATGTTGTAATAAGTGATGTGTGAAGTGTACTTAACATTTTCTCGGTTAATATGTTAAGTGTTAGGACATTTATGCATCTGCATGATCTGTTTTGTGGTACGAATCAAATGAATAAATCGAGTAGCTACATAGTTCTATAATTTGTATGATATTTACTTCGTCTCCTGTAACACTTCAGATCACCTCAAGACACTATTTCTTTTGTAAAGTTGGGGTGTGAGGGGTTGTGTGCGTTAGCTCCAATGACTATCTGCATCCGTGCCTCTTCATAGGATGAATCTCGAGTAATCCAACAAAACTATCAGGTCACACAAATCTAACCGCCCTCATTCCTAGCTCGATGGTACTCATATCGAAATGGACACTCCACAAATACCGTATCAACTACTATTGGCTTAAGAGGCTACCTTTGTCTTCCTAGGTTGTCGTGGTGGTCTCCTAGGTCCCTAGGGTGGTTTGACAGCTCTGAATTGGATTAACATGATCTCTAGGCTTCTCTGTTCAGACTCCTTGAACCTAGCTAGATCGAGAGAACTAAGGACTGAACCTGTTGATCGTCGCTTACGTTTTTAAGGTGCTCGAACGAACAACTGGCAGCATTCCATCCTAGATCATAGTCTAGCCAAACTGACTATCTGCACTCTACGGCGAGATCACACATTCATCACCTAGAGTTAATGTACATTAAATCATGCATACAGTCACACATGCTCAACGGGCGATAACATCCATCAAACACCCAGAGCACAGATCACAAATTTCTCATCATCTTCGTGATTACTTACATGACTATCACGTGCCTTCCCAAGTTCAATTGCTCAACATAAAAGTCACCAAAAACTCTAAAAATTCTCTGGAGAAGCCTAATTCAAGTCCGCCCAGGGTCTTCTCGAGTTAGTAAGAGGGGCAAAATCCCCTCGGTTGACTTTCATTGGTATTTTTCAGTTTTTCGCTATTTTCAATTTATCTTTTCTCGAGTCCCTAGCTCTATCCCAAACATTCTTTCCTTCTACACTTCCTTGAATTTCAACCTTTTAAGGCCCTCGATAAAATCTTTTAAGTTCAAAAATTAAACAAATACTTACATAATTGCACGACTCGAGCATTACAATGCATTTGGTAACAAACCCTAGTTGGTATAAGATTTTTGAAAGTTGGGTCGGAGTCCACCATATAGGTTATAGAGACGGGTTACAACGTAAACTTTAATGTGTGTTTACTTGACAAAACAGCAAACTCTCGTCACGATCAAATATACTTTGATAAAAGACAACAATTATATTATGTTTATGTAACGAGTTACGATTCCATTATATATATATATATATATATATATATATATAGTATTTAAGGTGTCATTTATATAAGTTACCATATTAGAATTTATGCATTTCATGCCAACAAATTTAATTTGATATATAACTTGGATTTCATATCACTTTACGTATATTTCATCATCTCTTAATCATATCTTATGATTTGTATGTTCCAAGAAATATGGAACAAATTTTGTTAATAACAACGTTGTTGTTGTTACTTTGTCTCAATGTTTCAAAAGCATTAGATTGTGAGTTGAATCCTAATGATCCTAATAGAGTTGCATATACTATTGTCGTTGACAAATCAGGTTCGGGAAATTTCAGAACAATCCAAAGTGCGATTGATTCGATCCCTTCACAAAATAGCCGATGGATTAGAATTCAAATTTCTAGTGGGATATACTCGTAAGATATCGTTTCGTTCATTTATCATTTTTCGTTCGTGTTGTGTGGCTAATTACTCATTCATTTTTTTATGATATTACCAAATTATTGATACGAGCCATAGATTGAAGTTTTAGATAGAACAATTTGAGAAGATCATCGTGATATAATAGGGAAAAGGTGCATATTTCGGAAGAAAAATCGTGCATCTTTCTCGAGGGAGCTGGTAGAAGTTCCACGGAAATTCGATGGAATGACCATGCAACCACAGCTACAAGTGCCACCTTCACTTCTATTGCAGAAAATCTTTGTAGTAAAAGGCATCACATTCAAGGTAGTATTGATATTATTTTTTTCATGTTGCAATATTTGTTAGCGATGAGCTTGAGCTGTTACAAATGGTATTAGAGCTAGACATCAGACGGTGTGCCAGCGAGGACGCTGGACCCTAGGGAGGTGGATTGTGAGATCTCACATCGGTTGGAGAGTGCATTCCTTAAAAGAGTGTAGAAACGTCTCCCTAGTGAACACGTCTTTTCTTAGTCTGAATAAGAAAAATGATAATATTTGTTAGCAGTAAACTTGGACTGTTATAACTTAATGACTTTTGCAGAATACGTATAATGTGCCCGGAAGTGTGAAGAGACAAGAAGATATAGCACCAGCAGTTGCAGCTCTCATAGAAGGAGATAAAGGGATTTTCCATAAGTGTGGGTTTGTTGGTCTGCAAGATACCTTGTGGGATGGAAATGGTCGCCATCGCTACACCGAATGCTACATCGAAGGTGTAATCGATGTCATATCTGGTGCTGGTCAATCTATATACGAGGTTAACTTTAAACTATGTTTTACGCATTTTATACGGTATGCATTACGTTGACAACCTCACGGTTTTAAAATAAAAAACACTTTTATAAGGAAGGTTTCGTTCCCCTTATCCAATCGATGTAGGATCTTACAATCCATCTTCTTTGGAGGCCCAGTGTCTTTGTTAGCACACCGCCCGGTGTCTGGCTCTGACACTATTTGTAACAGTTCAAGCTCACCACTAGTAGATATTGTACGTTTTGACTCATTACGTATCGTCGTCAGCCTCACGGTTTTAAAATGCATCTAGAAAGAGTCTATCTCTACTCCAACTAGTACCTCACACTTTTTGGTAACTTGGCTTTGATACCATTTATAATAGTCTAAGCCCACCGCTAGCAAATATTATCCGCTTTGACTCGTTATGTACCATCGCAACCTCATGGTTTTAAAACACATCCGCTGGAGAGAGGTTTCCACACCCTTATAAGGAATGTTTCATTCCCCTCCCCAACCGACGTGGGGATCTCACAATTATTCAATTGGTTTGAATTTCTTTTCATTTACGCAAGTGTTCGAGTTAGCTTACATGCATACTTACACCTTAAGTTAGACATTGAATTGGTTTGGAGTTGTGATTATTGTGGTCGTGTTGTTTACTGCAGAGCTGTGAGATAAATATACCAGTGGACCGATATGCTCCAGTGATAGGACATGGTTACATAACAGCTCAAGGGAAGGAAGATTCAAGTCAAACCAATGGGTTTGTATTCATTGGATGCTCAGTGATTGGAAGTGGGACTGTTTATCTTGGGAGGGCTTACAGACCTTTCTCCACAGTTATTTACTATTCATCTTTCCTATCAGCTTGTATTAACCCTGCTGGTTGGGATCCTTGGGAACAAGTTGGCCATGAGTAAGCCTTTTTTTTCTTCCCGTTCTTCGCTTCTCGGCAGTGAGACGAGACATTAACTCTAAATTGTTATGTGATGTGAATCTTGTAGGGCGAGTTTGACTTATTTAGAGACACGTTGTATTGGACCAGGAGCAGACACTTCGAAGCGTGTGCCTTGGCTTAAAAAACTTAGTATAGATGAGATTAGACGCTTTACGAGTATTTCTTTCGTTGATCAGGAAGGTTGGACATCGAAGCTTCCTCTATTT

mRNA sequence

GGTCTTATAATTTGTTTGTTCCAAGAAAAATGGAACATTACAATGACAAAATTTTGTTAATAACAACGTTGTGGTTGTTGTTACTTTGTCTCAATGTTTCAAACGCATTGGATTGTGAGTTGAATTTTAAAGATCCGAGTAGAGTTGCACGTACTATTTTTGTTCATCAGTTAGGGTCGGGAAATTTCAAAACAATCCAAAGTGCCATTGATTCGATCCCTCCACAAAATAATCGATGGATTAGAATTCTAATTTCTCCTGGCGTATATCGAAATCTCACTGAAATTCAATGGAATGACCATGAAACCACTGCTTCAAGTGCCACCTTCACTTTTTCTGCAGTAAATCTCATGGTACGAGGCATCTCGTTTAAGAATACTTATAATGCGCCGGGAAGTGTGACGTCACGTAATGATATAAAACCAGCACTTGCAGCTTTTGTAGACGGAGATAAAGCAGTCTTCCATCAGTGTGGCTTTTATGGTCTGCAAGATACTTTGTGGGACGGACCTGGTCGTCATCGGTACACCGAATGCTACATCGAAGGTGTAATCGATAAGTGTGTGATAAATATACCAATCAACGTATATGCTCCAATATTAGGACATGGTTACATAACAGCTCAAGGAAAGGAAGCTTCAAGCCATACCAATGGGTTTGTATTCATTGAATGCTCAGTGATTGGAAGTGGAACTGTTTATCTTGGGAGGGCTTATAGACGTTTCTCCACAGTTATTTATCATAAATCATTCCTATCATCTTGTGTTAACCCTGTTGGTTGGCATCCTTGGACACAAGTTGGCCACGAGATGGATTTTACGTATGTGGAATCAGGTTGCACTGGACCAGGAGCAGACATTTCGAAGCGTGTGCCTTGGATTAAGAATCTTAATAAATATGATCTTAGACGTTTCATCGATATTTCTTTTATTGATCAAGATGATTGTGAGTTGAATCCTAATGATCCTAATAGAGTTGCATATACTATTGTCGTTGACAAATCAGGTTCGGGAAATTTCAGAACAATCCAAAGTGCGATTGATTCGATCCCTTCACAAAATAGCCGATGGATTAGAATTCAAATTTCTAGTGGGATATACTCGTGCATATTTCGGAAGAAAAATCGTGCATCTTTCTCGAGGGAGCTGGTAGAAGTTCCACGGAAATTCGATGGAATGACCATGCAACCACAGCTACAAGTGCCACCTTCACTTCTATTGCAGAAAATCTTTGTAGTAAAAGGCATCACATTCAAGAATACGTATAATGTGCCCGGAAGTGTGAAGAGACAAGAAGATATAGCACCAGCAGTTGCAGCTCTCATAGAAGGAGATAAAGGGATTTTCCATAAGTGTGGGTTTGTTGGTCTGCAAGATACCTTGTGGGATGGAAATGGTCGCCATCGCTACACCGAATGCTACATCGAAGGTGTAATCGATGTCATATCTGGTGCTGGTCAATCTATATACGAGAGCTGTGAGATAAATATACCAGTGGACCGATATGCTCCAGTGATAGGACATGGTTACATAACAGCTCAAGGGAAGGAAGATTCAAGTCAAACCAATGGGTTTGTATTCATTGGATGCTCAGTGATTGGAAGTGGGACTGTTTATCTTGGGAGGGCTTACAGACCTTTCTCCACAGTTATTTACTATTCATCTTTCCTATCAGCTTGTATTAACCCTGCTGGTTGGGATCCTTGGGAACAAGTTGGCCATGAGGCGAGTTTGACTTATTTAGAGACACGTTGTATTGGACCAGGAGCAGACACTTCGAAGCGTGTGCCTTGGCTTAAAAAACTTAGTATAGATGAGATTAGACGCTTTACGAGTATTTCTTTCGTTGATCAGGAAGGTTGGACATCGAAGCTTCCTCTATTT

Coding sequence (CDS)

ATGGAACATTACAATGACAAAATTTTGTTAATAACAACGTTGTGGTTGTTGTTACTTTGTCTCAATGTTTCAAACGCATTGGATTGTGAGTTGAATTTTAAAGATCCGAGTAGAGTTGCACGTACTATTTTTGTTCATCAGTTAGGGTCGGGAAATTTCAAAACAATCCAAAGTGCCATTGATTCGATCCCTCCACAAAATAATCGATGGATTAGAATTCTAATTTCTCCTGGCGTATATCGAAATCTCACTGAAATTCAATGGAATGACCATGAAACCACTGCTTCAAGTGCCACCTTCACTTTTTCTGCAGTAAATCTCATGGTACGAGGCATCTCGTTTAAGAATACTTATAATGCGCCGGGAAGTGTGACGTCACGTAATGATATAAAACCAGCACTTGCAGCTTTTGTAGACGGAGATAAAGCAGTCTTCCATCAGTGTGGCTTTTATGGTCTGCAAGATACTTTGTGGGACGGACCTGGTCGTCATCGGTACACCGAATGCTACATCGAAGGTGTAATCGATAAGTGTGTGATAAATATACCAATCAACGTATATGCTCCAATATTAGGACATGGTTACATAACAGCTCAAGGAAAGGAAGCTTCAAGCCATACCAATGGGTTTGTATTCATTGAATGCTCAGTGATTGGAAGTGGAACTGTTTATCTTGGGAGGGCTTATAGACGTTTCTCCACAGTTATTTATCATAAATCATTCCTATCATCTTGTGTTAACCCTGTTGGTTGGCATCCTTGGACACAAGTTGGCCACGAGATGGATTTTACGTATGTGGAATCAGGTTGCACTGGACCAGGAGCAGACATTTCGAAGCGTGTGCCTTGGATTAAGAATCTTAATAAATATGATCTTAGACGTTTCATCGATATTTCTTTTATTGATCAAGATGATTGTGAGTTGAATCCTAATGATCCTAATAGAGTTGCATATACTATTGTCGTTGACAAATCAGGTTCGGGAAATTTCAGAACAATCCAAAGTGCGATTGATTCGATCCCTTCACAAAATAGCCGATGGATTAGAATTCAAATTTCTAGTGGGATATACTCGTGCATATTTCGGAAGAAAAATCGTGCATCTTTCTCGAGGGAGCTGGTAGAAGTTCCACGGAAATTCGATGGAATGACCATGCAACCACAGCTACAAGTGCCACCTTCACTTCTATTGCAGAAAATCTTTGTAGTAAAAGGCATCACATTCAAGAATACGTATAATGTGCCCGGAAGTGTGAAGAGACAAGAAGATATAGCACCAGCAGTTGCAGCTCTCATAGAAGGAGATAAAGGGATTTTCCATAAGTGTGGGTTTGTTGGTCTGCAAGATACCTTGTGGGATGGAAATGGTCGCCATCGCTACACCGAATGCTACATCGAAGGTGTAATCGATGTCATATCTGGTGCTGGTCAATCTATATACGAGAGCTGTGAGATAAATATACCAGTGGACCGATATGCTCCAGTGATAGGACATGGTTACATAACAGCTCAAGGGAAGGAAGATTCAAGTCAAACCAATGGGTTTGTATTCATTGGATGCTCAGTGATTGGAAGTGGGACTGTTTATCTTGGGAGGGCTTACAGACCTTTCTCCACAGTTATTTACTATTCATCTTTCCTATCAGCTTGTATTAACCCTGCTGGTTGGGATCCTTGGGAACAAGTTGGCCATGAGGCGAGTTTGACTTATTTAGAGACACGTTGTATTGGACCAGGAGCAGACACTTCGAAGCGTGTGCCTTGGCTTAAAAAACTTAGTATAGATGAGATTAGACGCTTTACGAGTATTTCTTTCGTTGATCAGGAAGGTTGGACATCGAAGCTTCCTCTATTT

Protein sequence

MEHYNDKILLITTLWLLLLCLNVSNALDCELNFKDPSRVARTIFVHQLGSGNFKTIQSAIDSIPPQNNRWIRILISPGVYRNLTEIQWNDHETTASSATFTFSAVNLMVRGISFKNTYNAPGSVTSRNDIKPALAAFVDGDKAVFHQCGFYGLQDTLWDGPGRHRYTECYIEGVIDKCVINIPINVYAPILGHGYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCVNPVGWHPWTQVGHEMDFTYVESGCTGPGADISKRVPWIKNLNKYDLRRFIDISFIDQDDCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYSCIFRKKNRASFSRELVEVPRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVDQEGWTSKLPLF
BLAST of Cp4.1LG02g16160 vs. Swiss-Prot
Match: PME55_ARATH (Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 6.0e-69
Identity = 130/307 (42.35%), Postives = 182/307 (59.28%), Query Frame = 1

Query: 318 YTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYSCIFRKKNRASFSRELVEV- 377
           + ++VD+SG GNF TIQ AIDS+P  N+ W  I + +G+Y    R+K      +  + + 
Sbjct: 27  FEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLY----REKITIPQKKPFIVIV 86

Query: 378 -----PRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVK-RQEDIAPAVAA 437
                  + +        Q P    L    VVK ITF N+YN P + K  +    PAVAA
Sbjct: 87  GAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAA 146

Query: 438 LIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINIPVD 497
            I GDK  F+  GF G+QDTLWD +GRH +  C I+G +D I G+GQSIY+SC I +   
Sbjct: 147 FIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGG 206

Query: 498 RYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSAC 557
           +  P +  GYITAQG+ +++  NGFVFI C V G G  YLGRA+RP+S VI+Y+S L+  
Sbjct: 207 QLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDV 266

Query: 558 INPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVDQEG 617
           ++P GW  W   G+E  LTY E  C G G++TS+R  W+KKLS   ++    +SF+++ G
Sbjct: 267 VDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGG 326

BLAST of Cp4.1LG02g16160 vs. Swiss-Prot
Match: PME66_ARATH (Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2)

HSP 1 Score: 260.4 bits (664), Expect = 5.1e-68
Identity = 138/310 (44.52%), Postives = 183/310 (59.03%), Query Frame = 1

Query: 315 RVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYSCIFRKKNRASFSRELV 374
           ++AYTI VD +G GNF T+QSAIDSI   N  WIR+   +GIY    R+K      +  +
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIY----REKVTIPKEKGFI 89

Query: 375 EVPRKFDGMTM-------QPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQEDIAPA 434
            +  K    T+          +    +     I V+ GITFKNTYN+  + KR  +I PA
Sbjct: 90  YLQGKGIEQTVIEYDDHQATDISATFTAFADDI-VISGITFKNTYNIVPNNKR--EIVPA 149

Query: 435 VAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINI 494
           VAA + GD+ +     FVGLQDTL+DG GRH Y  C I G ID I G GQS+++ C +N+
Sbjct: 150 VAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNM 209

Query: 495 PVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFL 554
            +  YAP   +G ITA  +   S   GFVF  C+V G G   LGRA+   + VI+  S L
Sbjct: 210 TLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRL 269

Query: 555 SACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVD 614
           S  + P GWD W   G+E  LT++E  C G GADTS+RVPWLKKLS+ E+  F S+SF+D
Sbjct: 270 SDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFID 329

Query: 615 QEGWTSKLPL 618
           Q+GW S+ P+
Sbjct: 330 QDGWISRFPI 332

BLAST of Cp4.1LG02g16160 vs. Swiss-Prot
Match: PME29_ARATH (Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 3.3e-67
Identity = 130/305 (42.62%), Postives = 177/305 (58.03%), Query Frame = 1

Query: 320 IVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYSCIFRKKNRASFSRELVEVPRK 379
           + VD+SG GNF TIQ AIDS+P  N  W  I + +G+Y    R+K +  + +  + +   
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLY----REKIKIPYEKPFIVLVGA 93

Query: 380 FDGMTM------QPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQEDI-APAVAALI 439
              +T           Q P    L    VVK ITF N+YN P   K  ++   PAVAALI
Sbjct: 94  GKRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALI 153

Query: 440 EGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINIPVDRY 499
            GDK  F+  GF G+QDTLWD +GRH +  C I+G +D I G GQSIY+SC I +   + 
Sbjct: 154 GGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQL 213

Query: 500 APVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACIN 559
            P +  GYITAQG+ +    NGF+FI C V G+G  +LGR +R +S VI+Y+S L+  + 
Sbjct: 214 EPGLA-GYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVV 273

Query: 560 PAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVDQEGWT 618
           P GWD W  VGHE  L + E  C G GA+  +RV W+KKLS   I+    +SF+++ GW 
Sbjct: 274 PEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWV 333

BLAST of Cp4.1LG02g16160 vs. Swiss-Prot
Match: PME10_ARATH (Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.4e-65
Identity = 135/307 (43.97%), Postives = 180/307 (58.63%), Query Frame = 1

Query: 314 NRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYS---CIFRKKNRASFS 373
           +++A TI+V+ + +  F+T+QSAIDSIP QN  WIRI IS+GIYS    I R K      
Sbjct: 35  SQIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQ 94

Query: 374 RELVEVPRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQEDIAPAVAA 433
              +E      G           +     I ++ GITFKN YN+  S        PAVAA
Sbjct: 95  GGGIEKTIIAYGDHQLTNTSATFTSYPSNI-IITGITFKNKYNIASS---SSPTKPAVAA 154

Query: 434 LIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINIPVD 493
           ++ GDK       F G QDTL+D  GRH Y  C I G ID I G  QSI+E C + + V 
Sbjct: 155 MMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVG 214

Query: 494 RYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSAC 553
            Y P   +G ITAQG++  +   GFVF  C+V+GSG   LGRA++ +S VI+Y S  S  
Sbjct: 215 IYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDN 274

Query: 554 INPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVDQEG 613
           I P GWD W+  G E  +T++E  C G GADTSKRVPWL K S  ++ +FT+++F+D+EG
Sbjct: 275 ILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEG 334

Query: 614 WTSKLPL 618
           W S+LP+
Sbjct: 335 WLSRLPI 337

BLAST of Cp4.1LG02g16160 vs. Swiss-Prot
Match: PME52_ARATH (Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2)

HSP 1 Score: 226.9 bits (577), Expect = 6.2e-58
Identity = 107/222 (48.20%), Postives = 140/222 (63.06%), Query Frame = 1

Query: 402 VVKGITFKNTYNVPGSVKRQE----DIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGR 461
           VV+ ++  NTYN   S+ +      DI PAVA  + GDK  F+ C F+GLQDT+WD  GR
Sbjct: 72  VVRNLSIMNTYNRLTSLTKANGMSWDIKPAVAISVYGDKSAFYNCDFLGLQDTVWDNLGR 131

Query: 462 HRYTECYIEGVIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVF 521
           H +  CYIEG ID I G+GQS+YE C IN      A  +  GYITAQG+   S  +GFVF
Sbjct: 132 HHFKNCYIEGAIDFIFGSGQSVYEDCHINATAGALASKVSFGYITAQGRSSDSDPSGFVF 191

Query: 522 IGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIG 581
           +  SV GS +VYLGRAY PFS VI+  + LS+ ++P GW  W   G+E S TY E  C G
Sbjct: 192 LRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVVHPEGWYSWHYGGYEMSFTYAEVECKG 251

Query: 582 PGADTSKRVPWLKKL-SIDEIRRFTSISFVDQEGWTSKLPLF 619
            G+D S+RVPW+ KL S    ++F+  +F+DQ+ W S +P F
Sbjct: 252 AGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQWISNIPRF 293

BLAST of Cp4.1LG02g16160 vs. TrEMBL
Match: B9RNC0_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1346520 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.8e-144
Identity = 292/671 (43.52%), Postives = 387/671 (57.68%), Query Frame = 1

Query: 10  LITTLWLLLLCLNVSNALDCELNFKDPSRVARTIFVHQLGSGNFKTIQSAIDSIPPQNNR 69
           L   + LLL   + S AL+C+ N    S+ ARTI V Q G+G+F ++QSAIDSIP  N +
Sbjct: 8   LFALVLLLLPFSSSSTALNCQPN---QSKFARTITVDQSGNGDFTSVQSAIDSIPEMNTQ 67

Query: 70  WIRILISPGVYRN-------------------LTEIQWNDHETTASSATFTFSAVNLMVR 129
           WI I ISPG YR                    LT I+W DHE T++SATFT    N++ +
Sbjct: 68  WIHIQISPGKYREKVTIPVKKPCIFLEGAGIRLTSIEWGDHEATSTSATFTSYPDNIVAK 127

Query: 130 GISFKNTYNAPGSVTSRN------DIKPALAAFVDGDKAVFHQCGFYGLQDTLWDGPGRH 189
           GI+FKNTYN    +T  N        + A++A + G++  F++C F G QDTLWD  GRH
Sbjct: 128 GITFKNTYNL--DITKINWWGEKIIWRQAVSARIKGEQCAFYKCAFLGTQDTLWDEKGRH 187

Query: 190 RYTECYIEGVID-----------KCVINIPINVYAPILGHGYITAQGKEASSHTNGFVFI 249
            ++ CYIEG ID            CVI++ I  Y P L  G ITAQ KE   H++GFVF 
Sbjct: 188 YFSNCYIEGAIDFIFGKAQSIYEGCVISVNIGKYPPGL-QGCITAQKKEWPQHSSGFVFK 247

Query: 250 ECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCVNPVGWHPWTQVGHEMDFTYVESGCTGP 309
            C V G+G  +LGRA+  +STVI++ S LS  +   GW+ W  V HE +FTY E+   G 
Sbjct: 248 NCVVSGTGKAFLGRAWGPYSTVIFYNSTLSDVIVSEGWNAWNYVHHEANFTYAEANNRGV 307

Query: 310 GADISKRVPWIKNLNKYDLRRFIDISFIDQD------------------DCELNPNDPNR 369
           GAD SKRVPW K LN   LRRF+D+SF+D                    DC+L   +  +
Sbjct: 308 GADTSKRVPWEKKLNADQLRRFLDLSFVDGGGWLAKIPKLHYPAACKALDCQLQ--EFRK 367

Query: 370 VAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYS----------CIFRKKN 429
           VA+T  VDKSG GNF TIQSAI+S+P  N++W+ IQIS   Y           CIF K  
Sbjct: 368 VAFTYTVDKSGKGNFTTIQSAINSVPEGNTQWLCIQISPEKYREKVTIPENKPCIFLKG- 427

Query: 430 RASFSRELVEVPRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQEDIA 489
            A     ++E        T         ++      + KGITFKNTYN+     R  D  
Sbjct: 428 -AGRKLTIIEWGDHETTNTSATFSSYSDNI------IAKGITFKNTYNLLQKPDRV-DWK 487

Query: 490 PAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEI 549
            AV+A I GDK  F++C F+G+QDTLWD  GRH + +C+IEG +D I G  +S+YE C I
Sbjct: 488 QAVSARIRGDKCAFYRCAFLGVQDTLWDEKGRHLFKKCFIEGAVDFIFGKAKSVYERCLI 547

Query: 550 NIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSS 609
            + + RY P +  GYITAQ KE +   +GFVF    + G+G  YLGRA+ P+STVI  ++
Sbjct: 548 YVNIGRYEPEL-EGYITAQKKELTDHESGFVFKDSEINGTGKAYLGRAWGPYSTVIIQNT 607

Query: 610 FLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISF 617
            LS  + P GW+ W+ V  E + TY+E    G GA+TS RVPWLKKL+ DE+ +F S+SF
Sbjct: 608 TLSDVVVPQGWNAWDYVQQEENFTYVEVDNKGAGANTSNRVPWLKKLNADELSKFLSMSF 660

BLAST of Cp4.1LG02g16160 vs. TrEMBL
Match: A0A0A0LB12_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 7.5e-111
Identity = 197/336 (58.63%), Postives = 239/336 (71.13%), Query Frame = 1

Query: 1   MEHYNDK---ILLITTLWLLLLCLNVSNALD-CELNFKDPSRVARTIFVHQLGSGNFKTI 60
           MEHY  K   + +ITTL ++L C+ VS ALD CELN+ DP +VA TI V +LG GNF TI
Sbjct: 1   MEHYYSKEISLFIITTL-VVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTI 60

Query: 61  QSAIDSIPPQNNRWIRILISPGVYR-------------------NLTEIQWNDHETTASS 120
           QSAI+S+P Q+ +WIRI ISPG+Y                     +TEI WNDHETTA+S
Sbjct: 61  QSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAAS 120

Query: 121 ATFTFSAVNLMVRGISFKNTYNAPGSVTSRNDIKPALAAFVDGDKAVFHQCGFYGLQDTL 180
            TFT SA NL+V+GI+F+NTYNA GSV  R DIKPALAA + GDK +FH+CGF GLQDTL
Sbjct: 121 PTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTL 180

Query: 181 WDGPGRHRYTECYIEGVID-----------KCVINIPINVYAPILGHGYITAQGKEASSH 240
           WDGPGRH +T+CYIEGVID           +CVINIP+N YAP+L  G+ITAQGKE  + 
Sbjct: 181 WDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNE 240

Query: 241 TNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCVNPVGWHPWTQVGHEMDFTYV 300
           ++GFVF+ C V GSG V+LGRAYR FSTVI+H  FL SC+NP GW+ W Q GHE D TY 
Sbjct: 241 SSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYS 300

Query: 301 ESGCTGPGADISKRVPWIKNLNKYDLRRFIDISFID 303
           E+ C GPGAD S RVPW+  L+ + +R F DIS+ID
Sbjct: 301 ETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYID 335

BLAST of Cp4.1LG02g16160 vs. TrEMBL
Match: A0A151U2J1_CAJCA (Pectinesterase (Fragment) OS=Cajanus cajan GN=KK1_006073 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.2e-84
Identity = 185/510 (36.27%), Postives = 270/510 (52.94%), Query Frame = 1

Query: 130 IKPALAAFVDGDKAVFHQCGFYGLQDTLWDGPGRHRYTECYIEGVID-----------KC 189
           I PA+A  + GDK+   +C F G QDTL+D  GRH Y  CYI+G ID            C
Sbjct: 5   ITPAVAVTIYGDKSFILRCSFIGYQDTLFDAEGRHYYKNCYIQGEIDFIFGGGQSYFENC 64

Query: 190 VINIP-INVYAPILGHGYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIY 249
            +N    N   P    G++TAQ +++ ++ +GFVF   S++G+G V LGRA+R +S VI+
Sbjct: 65  SLNATGRNKDLP----GFVTAQARDSPNNPSGFVFEGGSLVGNGAVNLGRAWRTYSRVIF 124

Query: 250 HKSFLSSCVNPVGWHPWTQVGHEMDFTYVESGCTGPGADISKRVPWIKNLNKYDLRRFID 309
           HK                        TY  S  T  G        W  N       + ID
Sbjct: 125 HK------------------------TYFSSVVTPQG--------W-NNFGHDGQDKAID 184

Query: 310 ISFIDQDDCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIY 369
               D             V+YTI V  SG   F+TIQ+AIDS+ + N++W++I I +G Y
Sbjct: 185 CGGKD-------------VSYTITVGSSGKVTFKTIQAAIDSVKNNNNQWVKIHIKAGSY 244

Query: 370 ----------SCIFRKKNRASFSRELVEVPRKFDGMTMQPQLQVPPSLLLQKIFVVKGIT 429
                      CI  +   +     ++         +    +  PP++      V+  IT
Sbjct: 245 IEKVHIPIEKPCIILEGEGSQ--NTIISFSDNKGTSSSATFVSSPPNV------VMSDIT 304

Query: 430 FKNTYNVPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEG 489
           F+NTYNV    ++   ++PAVAALI+GDK    +C F+G QDTL+D  GRH Y +CYI+G
Sbjct: 305 FQNTYNVNNKTQK---VSPAVAALIQGDKSFIFRCSFIGYQDTLFDAYGRHYYKDCYIQG 364

Query: 490 VIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGT 549
            +D I G  QS YE+C +N    R  P    G++TAQG++     +GFVF G S++G+  
Sbjct: 365 EVDFIFGRAQSYYENCLLNA-TGRNLP----GFVTAQGRDSPDSPSGFVFEGGSLVGNDK 424

Query: 550 VYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVP 609
           V LGRA+R +S VI++++  S+ + P GW+ + + G E+ +TY E  C GPGADTSKRVP
Sbjct: 425 VNLGRAWRAYSRVIFHNTNFSSVVTPQGWNSFGRAGQESKITYAEVDCKGPGADTSKRVP 448

Query: 610 WLKKLSIDEIRRFTSISFVDQEGWTSKLPL 618
           WLKKL+  ++  F+  SF++++ W   LP+
Sbjct: 485 WLKKLTPSQLEEFSLASFINKDNWLDNLPV 448

BLAST of Cp4.1LG02g16160 vs. TrEMBL
Match: A0A061DSC1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.6e-81
Identity = 153/322 (47.52%), Postives = 200/322 (62.11%), Query Frame = 1

Query: 305 DCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYS------ 364
           +C+LN  +  +VA+TI VD+SG G+F+TIQ+AIDSIPS N +WIRI IS G +       
Sbjct: 41  ECQLNNANKFKVAHTIRVDQSGKGDFKTIQNAIDSIPSNNDKWIRILISPGHFREKVTIP 100

Query: 365 ----CIFRKKNRASFSRELVEVPRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNV 424
               CIF +   +  +   +E     D          P ++      V KGI+FKN YN+
Sbjct: 101 ANKPCIFLEGAGSKLTS--IEWGDHMDTCDSATFTSSPDNI------VAKGISFKNIYNI 160

Query: 425 PGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISG 484
           P     ++ + PA+A  I GDK  F+ C F GLQDTLWD  GRH +  CYIEG +D I G
Sbjct: 161 PSGPDAKKKVEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQGRHYFYNCYIEGAVDFIFG 220

Query: 485 AGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAY 544
           +GQSIYE CEIN+   +YAP   +GYITAQG+  S   +GFVF  C   G G  YLGRAY
Sbjct: 221 SGQSIYEKCEINLTAGKYAPEYPNGYITAQGRNSSDDPSGFVFKYCVFTGYGKTYLGRAY 280

Query: 545 RPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSI 604
             +S VI Y S LS  I  +GWD W  V HE +L Y+E  C GPGA+TSKRVPWLKKL  
Sbjct: 281 GAYSRVIIYKSVLSDAILASGWDAWRYVHHEGNLIYVEADCKGPGANTSKRVPWLKKLGA 340

Query: 605 DEIRRFTSISFVDQEGWTSKLP 617
            ++++F ++S++D+EGW +KLP
Sbjct: 341 SQLKKFLNLSYIDKEGWIAKLP 354

BLAST of Cp4.1LG02g16160 vs. TrEMBL
Match: V4VWS4_9ROSI (Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.4e-80
Identity = 150/317 (47.32%), Postives = 193/317 (60.88%), Query Frame = 1

Query: 305 DCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYS--CIFR 364
           DC+LN  +P +VAYTI V +SG GNF  IQ AI+ IP  N +WIRIQIS  IY       
Sbjct: 2   DCKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPVNNDQWIRIQISPEIYQEKVTIP 61

Query: 365 KKNRASFSRELVEVPRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQE 424
           +     F         K         +  P    L +  V KGI F+NTYNVP S ++ +
Sbjct: 62  QNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSENVVAKGILFQNTYNVPPSYRQNQ 121

Query: 425 D---IAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSI 484
           +   +  A+AA I GDK  F+ CGF GLQDTL+D +GRH + +CYIEG ID I G GQSI
Sbjct: 122 ERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQCYIEGAIDFIFGNGQSI 181

Query: 485 YESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFST 544
           Y +C +N+ +  Y P   +GYITAQG+  +   +GFVF  C  +GSG  YLGRAY  FS 
Sbjct: 182 YHNCAVNVTLKEYLPEKDYGYITAQGRNSTDDPSGFVFTSCQFLGSGKAYLGRAYGAFSR 241

Query: 545 VIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRR 604
           VI  +S L+  + P GW+ W  V HE ++ Y E RC G GADTSKRV W K LS+DE+++
Sbjct: 242 VIIVNSVLTDMVEPQGWNAWNHVEHEENIEYAEARCRGAGADTSKRVSWEKHLSVDEVKK 301

Query: 605 FTSISFVDQEGWTSKLP 617
           FT  SF+D +GW +KLP
Sbjct: 302 FTDSSFIDHDGWIAKLP 318

BLAST of Cp4.1LG02g16160 vs. TAIR10
Match: AT5G18990.1 (AT5G18990.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 263.5 bits (672), Expect = 3.4e-70
Identity = 130/307 (42.35%), Postives = 182/307 (59.28%), Query Frame = 1

Query: 318 YTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYSCIFRKKNRASFSRELVEV- 377
           + ++VD+SG GNF TIQ AIDS+P  N+ W  I + +G+Y    R+K      +  + + 
Sbjct: 27  FEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLY----REKITIPQKKPFIVIV 86

Query: 378 -----PRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVK-RQEDIAPAVAA 437
                  + +        Q P    L    VVK ITF N+YN P + K  +    PAVAA
Sbjct: 87  GAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAA 146

Query: 438 LIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINIPVD 497
            I GDK  F+  GF G+QDTLWD +GRH +  C I+G +D I G+GQSIY+SC I +   
Sbjct: 147 FIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGG 206

Query: 498 RYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSAC 557
           +  P +  GYITAQG+ +++  NGFVFI C V G G  YLGRA+RP+S VI+Y+S L+  
Sbjct: 207 QLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDV 266

Query: 558 INPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVDQEG 617
           ++P GW  W   G+E  LTY E  C G G++TS+R  W+KKLS   ++    +SF+++ G
Sbjct: 267 VDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGG 326

BLAST of Cp4.1LG02g16160 vs. TAIR10
Match: AT2G47280.1 (AT2G47280.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 260.4 bits (664), Expect = 2.8e-69
Identity = 138/310 (44.52%), Postives = 183/310 (59.03%), Query Frame = 1

Query: 315 RVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYSCIFRKKNRASFSRELV 374
           ++AYTI VD +G GNF T+QSAIDSI   N  WIR+   +GIY    R+K      +  +
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIY----REKVTIPKEKGFI 89

Query: 375 EVPRKFDGMTM-------QPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQEDIAPA 434
            +  K    T+          +    +     I V+ GITFKNTYN+  + KR  +I PA
Sbjct: 90  YLQGKGIEQTVIEYDDHQATDISATFTAFADDI-VISGITFKNTYNIVPNNKR--EIVPA 149

Query: 435 VAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINI 494
           VAA + GD+ +     FVGLQDTL+DG GRH Y  C I G ID I G GQS+++ C +N+
Sbjct: 150 VAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNM 209

Query: 495 PVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFL 554
            +  YAP   +G ITA  +   S   GFVF  C+V G G   LGRA+   + VI+  S L
Sbjct: 210 TLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRL 269

Query: 555 SACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVD 614
           S  + P GWD W   G+E  LT++E  C G GADTS+RVPWLKKLS+ E+  F S+SF+D
Sbjct: 270 SDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFID 329

Query: 615 QEGWTSKLPL 618
           Q+GW S+ P+
Sbjct: 330 QDGWISRFPI 332

BLAST of Cp4.1LG02g16160 vs. TAIR10
Match: AT3G24130.1 (AT3G24130.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-68
Identity = 130/305 (42.62%), Postives = 177/305 (58.03%), Query Frame = 1

Query: 320 IVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYSCIFRKKNRASFSRELVEVPRK 379
           + VD+SG GNF TIQ AIDS+P  N  W  I + +G+Y    R+K +  + +  + +   
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLY----REKIKIPYEKPFIVLVGA 93

Query: 380 FDGMTM------QPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQEDI-APAVAALI 439
              +T           Q P    L    VVK ITF N+YN P   K  ++   PAVAALI
Sbjct: 94  GKRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALI 153

Query: 440 EGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINIPVDRY 499
            GDK  F+  GF G+QDTLWD +GRH +  C I+G +D I G GQSIY+SC I +   + 
Sbjct: 154 GGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQL 213

Query: 500 APVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACIN 559
            P +  GYITAQG+ +    NGF+FI C V G+G  +LGR +R +S VI+Y+S L+  + 
Sbjct: 214 EPGLA-GYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVV 273

Query: 560 PAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVDQEGWT 618
           P GWD W  VGHE  L + E  C G GA+  +RV W+KKLS   I+    +SF+++ GW 
Sbjct: 274 PEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWV 333

BLAST of Cp4.1LG02g16160 vs. TAIR10
Match: AT2G19150.1 (AT2G19150.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 252.3 bits (643), Expect = 7.7e-67
Identity = 135/307 (43.97%), Postives = 180/307 (58.63%), Query Frame = 1

Query: 314 NRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYS---CIFRKKNRASFS 373
           +++A TI+V+ + +  F+T+QSAIDSIP QN  WIRI IS+GIYS    I R K      
Sbjct: 35  SQIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQ 94

Query: 374 RELVEVPRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQEDIAPAVAA 433
              +E      G           +     I ++ GITFKN YN+  S        PAVAA
Sbjct: 95  GGGIEKTIIAYGDHQLTNTSATFTSYPSNI-IITGITFKNKYNIASS---SSPTKPAVAA 154

Query: 434 LIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINIPVD 493
           ++ GDK       F G QDTL+D  GRH Y  C I G ID I G  QSI+E C + + V 
Sbjct: 155 MMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVG 214

Query: 494 RYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSAC 553
            Y P   +G ITAQG++  +   GFVF  C+V+GSG   LGRA++ +S VI+Y S  S  
Sbjct: 215 IYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDN 274

Query: 554 INPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVDQEG 613
           I P GWD W+  G E  +T++E  C G GADTSKRVPWL K S  ++ +FT+++F+D+EG
Sbjct: 275 ILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEG 334

Query: 614 WTSKLPL 618
           W S+LP+
Sbjct: 335 WLSRLPI 337

BLAST of Cp4.1LG02g16160 vs. TAIR10
Match: AT5G26810.1 (AT5G26810.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 226.9 bits (577), Expect = 3.5e-59
Identity = 107/222 (48.20%), Postives = 140/222 (63.06%), Query Frame = 1

Query: 402 VVKGITFKNTYNVPGSVKRQE----DIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGR 461
           VV+ ++  NTYN   S+ +      DI PAVA  + GDK  F+ C F+GLQDT+WD  GR
Sbjct: 72  VVRNLSIMNTYNRLTSLTKANGMSWDIKPAVAISVYGDKSAFYNCDFLGLQDTVWDNLGR 131

Query: 462 HRYTECYIEGVIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVF 521
           H +  CYIEG ID I G+GQS+YE C IN      A  +  GYITAQG+   S  +GFVF
Sbjct: 132 HHFKNCYIEGAIDFIFGSGQSVYEDCHINATAGALASKVSFGYITAQGRSSDSDPSGFVF 191

Query: 522 IGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIG 581
           +  SV GS +VYLGRAY PFS VI+  + LS+ ++P GW  W   G+E S TY E  C G
Sbjct: 192 LRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVVHPEGWYSWHYGGYEMSFTYAEVECKG 251

Query: 582 PGADTSKRVPWLKKL-SIDEIRRFTSISFVDQEGWTSKLPLF 619
            G+D S+RVPW+ KL S    ++F+  +F+DQ+ W S +P F
Sbjct: 252 AGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQWISNIPRF 293

BLAST of Cp4.1LG02g16160 vs. NCBI nr
Match: gi|1009147929|ref|XP_015891671.1| (PREDICTED: uncharacterized protein LOC107426085 [Ziziphus jujuba])

HSP 1 Score: 556.2 bits (1432), Expect = 7.1e-155
Identity = 298/700 (42.57%), Postives = 388/700 (55.43%), Query Frame = 1

Query: 3   HYNDKILLITTLWLLLLCLNVSNALDCELNFKDPSRVARTIFVHQLGSGNFKTIQSAIDS 62
           HY + + +I  L ++   ++V  ALDC LN  DP  VA T+ V + G+GNF TIQ AIDS
Sbjct: 2   HYPNILAIIYALLVMHYFVSVCRALDCSLNGSDPVNVAYTLTVDKSGNGNFTTIQEAIDS 61

Query: 63  IPPQNNRWIRILISPGVYRN-------------------LTEIQWNDHETTASSATFTFS 122
           IP  N++W+RI ++ G Y                      T I+WNDHE T +SATFT  
Sbjct: 62  IPLSNSQWVRISVASGTYTEKVTIQYNKTCIYLEGAGSQFTSIEWNDHEQTDTSATFTSF 121

Query: 123 AVNLMVRGISFKNTYNAPGSVTSRNDIKPALAAFVDGDKAVFHQCGFYGLQDTLWDGPGR 182
           + N + +GI+FKNTY     V    + KPALAA + GDK+ F+ C F GLQDTLWD  GR
Sbjct: 122 SDNFVAKGITFKNTY-----VQDVFNYKPALAARIYGDKSAFYHCSFVGLQDTLWDATGR 181

Query: 183 HRYTECYIEGVID-----------KCVINIPINVYAPILGHGYITAQGKEASSHTNGFVF 242
           H Y  CY EGV+D            C+IN+ + +YAPI   G ITAQG+++    NGFVF
Sbjct: 182 HYYNNCYFEGVVDFIFGYARSIFQGCLINVTVGIYAPIKSPGSITAQGRKSKEENNGFVF 241

Query: 243 IECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCVNPVGWHPWTQVGHEMDFTYVESGCTG 302
            +C + G+G  Y+GRAY  +STVI + S LS  ++P GW  W   G E +F YVE+   G
Sbjct: 242 KDCVIEGTGKAYIGRAYGAYSTVIVYNSQLSDVIDPRGWSSWLFPGQEENFLYVEASNRG 301

Query: 303 PGADISKRVPWIKNLNKYDLRRFIDISFIDQD---------------------------- 362
             AD SKRVPW+K LN   L +F     + Q+                            
Sbjct: 302 AEADTSKRVPWLKTLNDDQLNQFFMGKPVRQEKEKVKRKKRIKEMQNFELFSVCIIFILL 361

Query: 363 ----------DCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISS 422
                     DC LN     +VA TI VDKSG   F TIQSAIDS+P  N +WI+IQIS 
Sbjct: 362 VHCLSVCKGLDCTLNGG---QVAKTINVDKSGQAEFATIQSAIDSVPPNNKQWIKIQISH 421

Query: 423 GIY----------SCIFRKKNRASFS------RELVEVPRKFDGMTMQPQLQVPPSLLLQ 482
           G++          SCI+     + F+       EL      F                  
Sbjct: 422 GVFREKVNIPGDKSCIYLNGGGSKFTFIEWNDHELTGQSATFTSNGEN------------ 481

Query: 483 KIFVVKGITFKNTYNVP-GSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGR 542
              V +GITF NTYN P    + Q ++  AVAA I GDK  F+ C F G+QDTL+D +GR
Sbjct: 482 --IVARGITFVNTYNSPLNKGEEQHNLTQAVAAFINGDKTAFYHCSFAGVQDTLYDQSGR 541

Query: 543 HRYTECYIEGVIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVF 602
           H + ECYI+G +D I G GQSIYE C +N     Y P    GYITAQG+E  S+T+GFVF
Sbjct: 542 HYFRECYIQGAVDFIFGNGQSIYEGCTLNYSTGVYGPPNSLGYITAQGRESESETSGFVF 601

Query: 603 IGCSVIGS-GTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCI 617
             C+V G+ G  YLGRAYR +S VI+ +S L+  + P GWD W  VGHE  LT+ E  CI
Sbjct: 602 KSCTVTGTRGKTYLGRAYRAYSRVIFANSVLTDVVQPLGWDAWFHVGHEEQLTFAEANCI 661

BLAST of Cp4.1LG02g16160 vs. NCBI nr
Match: gi|802700695|ref|XP_012083804.1| (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase 30 [Jatropha curcas])

HSP 1 Score: 525.4 bits (1352), Expect = 1.3e-145
Identity = 289/677 (42.69%), Postives = 387/677 (57.16%), Query Frame = 1

Query: 9   LLITTLWLLLLCLNVSNALDCELNFKDPSRVARTIFVHQLGSGNFKTIQSAIDSIPPQNN 68
           L    L+ LL C + S A DC+ +    + VA TI V + G GNF ++QSAI+ IP +N 
Sbjct: 8   LFAVLLFALLHCWSGSEASDCQYS---KNHVAYTITVDKSGKGNFSSVQSAINHIPERNT 67

Query: 69  RWIRILISPGVY-------------------RNLTEIQWNDHETTASSATFTFSAVNLMV 128
           RWIRI IS G Y                   R+LT I+W DH+ T++SATFT +  N++ 
Sbjct: 68  RWIRIRISSGKYNEKVTVPARKPCIVFEGAGRHLTSIEWGDHQDTSTSATFTSNPDNIVA 127

Query: 129 RGISFKNTYN-APGSVTSRNDIKPALAAFVDGDKAVFHQCGFYGLQDTLWDGPGRHRYTE 188
           +GI+FKNTYN     +T+    + A++A + GDK  F+ C F GLQDTLWD  G H +  
Sbjct: 128 KGITFKNTYNWGKAKITNW---RQAVSARLKGDKYAFYDCAFLGLQDTLWDENGLHYFRG 187

Query: 189 CYIEGVID-----------KCVINIPINVYAPILGHGYITAQGKEASSHTNGFVFIECSV 248
           CYIEG +D           +CVI++ I  Y      G ITAQ KE   + +GFVF  C +
Sbjct: 188 CYIEGAVDFIFGKAQSIYEECVISVNIGKYEKE-SKGSITAQKKEWPEYKSGFVFKNCVI 247

Query: 249 IGSGTVYLGRAYRRFSTVIYHKSFLSSCVNPVGWHPWTQVGHEMDFTYVESGCTGPGADI 308
            G+G   LGRA+  +STVI + SFLS  V+P GW  W   GHE + TYVE+   G GA+ 
Sbjct: 248 NGTGKAILGRAWGPYSTVIIYNSFLSEVVDPKGWDAWEYYGHEANLTYVEANNNGEGANT 307

Query: 309 SKRVPWIKNLNKYDLRRFIDISFIDQD----------------------------DCELN 368
           S RV W+K L+  +L +F+DISFI+ +                            DC+LN
Sbjct: 308 SMRVSWLKKLSSNELNKFLDISFINGNEWLDKIPTSFNXYXLIHHLYFLFVCKALDCQLN 367

Query: 369 PNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIY----------SC 428
            ++  +VAYTI VDKSG GNF TIQSAIDS+P  N+ WIRIQIS   Y           C
Sbjct: 368 KSNSLKVAYTISVDKSGKGNFTTIQSAIDSVPDGNTHWIRIQISPNTYREKITIPMKKPC 427

Query: 429 IFRKKNRASFSRELVEVPRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVK 488
           IF +       +EL  +     G+T         S    +  + KGI FKNTYN    VK
Sbjct: 428 IFLE----GADKELTSIEWDDHGVTGTSATFSSYS----ENLIAKGIKFKNTYNWNLPVK 487

Query: 489 RQE-DIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQS 548
            +      AV+A I GDK  F+ C FVG+QDT WD  GRH + +CY EG +D I G  +S
Sbjct: 488 AENFSWRQAVSARIRGDKSAFYDCAFVGIQDTFWDEKGRHYFYKCYFEGAVDFIFGKAKS 547

Query: 549 IYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFS 608
           IYE C +++   RY P I  GYITAQ KE +   +GFVF  C + GSG  YLGRA+ PFS
Sbjct: 548 IYEKCLLSVNKGRYDPEI-PGYITAQKKEWAEHESGFVFKNCEINGSGKAYLGRAWGPFS 607

Query: 609 TVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIR 616
           +V+ Y+S +S  I P GW+ W+ VGHEA+ TY+E    GPGA+TSKRVPW+ +L+  E+ 
Sbjct: 608 SVVIYNSTISEVIVPQGWNAWDYVGHEANFTYVEVDNRGPGAETSKRVPWVNQLNASELS 667

BLAST of Cp4.1LG02g16160 vs. NCBI nr
Match: gi|223545739|gb|EEF47243.1| (pectinesterase, putative [Ricinus communis])

HSP 1 Score: 521.2 bits (1341), Expect = 2.5e-144
Identity = 292/671 (43.52%), Postives = 387/671 (57.68%), Query Frame = 1

Query: 10  LITTLWLLLLCLNVSNALDCELNFKDPSRVARTIFVHQLGSGNFKTIQSAIDSIPPQNNR 69
           L   + LLL   + S AL+C+ N    S+ ARTI V Q G+G+F ++QSAIDSIP  N +
Sbjct: 8   LFALVLLLLPFSSSSTALNCQPN---QSKFARTITVDQSGNGDFTSVQSAIDSIPEMNTQ 67

Query: 70  WIRILISPGVYRN-------------------LTEIQWNDHETTASSATFTFSAVNLMVR 129
           WI I ISPG YR                    LT I+W DHE T++SATFT    N++ +
Sbjct: 68  WIHIQISPGKYREKVTIPVKKPCIFLEGAGIRLTSIEWGDHEATSTSATFTSYPDNIVAK 127

Query: 130 GISFKNTYNAPGSVTSRN------DIKPALAAFVDGDKAVFHQCGFYGLQDTLWDGPGRH 189
           GI+FKNTYN    +T  N        + A++A + G++  F++C F G QDTLWD  GRH
Sbjct: 128 GITFKNTYNL--DITKINWWGEKIIWRQAVSARIKGEQCAFYKCAFLGTQDTLWDEKGRH 187

Query: 190 RYTECYIEGVID-----------KCVINIPINVYAPILGHGYITAQGKEASSHTNGFVFI 249
            ++ CYIEG ID            CVI++ I  Y P L  G ITAQ KE   H++GFVF 
Sbjct: 188 YFSNCYIEGAIDFIFGKAQSIYEGCVISVNIGKYPPGL-QGCITAQKKEWPQHSSGFVFK 247

Query: 250 ECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCVNPVGWHPWTQVGHEMDFTYVESGCTGP 309
            C V G+G  +LGRA+  +STVI++ S LS  +   GW+ W  V HE +FTY E+   G 
Sbjct: 248 NCVVSGTGKAFLGRAWGPYSTVIFYNSTLSDVIVSEGWNAWNYVHHEANFTYAEANNRGV 307

Query: 310 GADISKRVPWIKNLNKYDLRRFIDISFIDQD------------------DCELNPNDPNR 369
           GAD SKRVPW K LN   LRRF+D+SF+D                    DC+L   +  +
Sbjct: 308 GADTSKRVPWEKKLNADQLRRFLDLSFVDGGGWLAKIPKLHYPAACKALDCQLQ--EFRK 367

Query: 370 VAYTIVVDKSGSGNFRTIQSAIDSIPSQNSRWIRIQISSGIYS----------CIFRKKN 429
           VA+T  VDKSG GNF TIQSAI+S+P  N++W+ IQIS   Y           CIF K  
Sbjct: 368 VAFTYTVDKSGKGNFTTIQSAINSVPEGNTQWLCIQISPEKYREKVTIPENKPCIFLKG- 427

Query: 430 RASFSRELVEVPRKFDGMTMQPQLQVPPSLLLQKIFVVKGITFKNTYNVPGSVKRQEDIA 489
            A     ++E        T         ++      + KGITFKNTYN+     R  D  
Sbjct: 428 -AGRKLTIIEWGDHETTNTSATFSSYSDNI------IAKGITFKNTYNLLQKPDRV-DWK 487

Query: 490 PAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAGQSIYESCEI 549
            AV+A I GDK  F++C F+G+QDTLWD  GRH + +C+IEG +D I G  +S+YE C I
Sbjct: 488 QAVSARIRGDKCAFYRCAFLGVQDTLWDEKGRHLFKKCFIEGAVDFIFGKAKSVYERCLI 547

Query: 550 NIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSS 609
            + + RY P +  GYITAQ KE +   +GFVF    + G+G  YLGRA+ P+STVI  ++
Sbjct: 548 YVNIGRYEPEL-EGYITAQKKELTDHESGFVFKDSEINGTGKAYLGRAWGPYSTVIIQNT 607

Query: 610 FLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISF 617
            LS  + P GW+ W+ V  E + TY+E    G GA+TS RVPWLKKL+ DE+ +F S+SF
Sbjct: 608 TLSDVVVPQGWNAWDYVQQEENFTYVEVDNKGAGANTSNRVPWLKKLNADELSKFLSMSF 660

BLAST of Cp4.1LG02g16160 vs. NCBI nr
Match: gi|700203001|gb|KGN58134.1| (hypothetical protein Csa_3G536660 [Cucumis sativus])

HSP 1 Score: 409.5 bits (1051), Expect = 1.1e-110
Identity = 197/336 (58.63%), Postives = 239/336 (71.13%), Query Frame = 1

Query: 1   MEHYNDK---ILLITTLWLLLLCLNVSNALD-CELNFKDPSRVARTIFVHQLGSGNFKTI 60
           MEHY  K   + +ITTL ++L C+ VS ALD CELN+ DP +VA TI V +LG GNF TI
Sbjct: 1   MEHYYSKEISLFIITTL-VVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTI 60

Query: 61  QSAIDSIPPQNNRWIRILISPGVYR-------------------NLTEIQWNDHETTASS 120
           QSAI+S+P Q+ +WIRI ISPG+Y                     +TEI WNDHETTA+S
Sbjct: 61  QSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAAS 120

Query: 121 ATFTFSAVNLMVRGISFKNTYNAPGSVTSRNDIKPALAAFVDGDKAVFHQCGFYGLQDTL 180
            TFT SA NL+V+GI+F+NTYNA GSV  R DIKPALAA + GDK +FH+CGF GLQDTL
Sbjct: 121 PTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTL 180

Query: 181 WDGPGRHRYTECYIEGVID-----------KCVINIPINVYAPILGHGYITAQGKEASSH 240
           WDGPGRH +T+CYIEGVID           +CVINIP+N YAP+L  G+ITAQGKE  + 
Sbjct: 181 WDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNE 240

Query: 241 TNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCVNPVGWHPWTQVGHEMDFTYV 300
           ++GFVF+ C V GSG V+LGRAYR FSTVI+H  FL SC+NP GW+ W Q GHE D TY 
Sbjct: 241 SSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYS 300

Query: 301 ESGCTGPGADISKRVPWIKNLNKYDLRRFIDISFID 303
           E+ C GPGAD S RVPW+  L+ + +R F DIS+ID
Sbjct: 301 ETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYID 335

BLAST of Cp4.1LG02g16160 vs. NCBI nr
Match: gi|659093698|ref|XP_008447669.1| (PREDICTED: putative pectinesterase 52 [Cucumis melo])

HSP 1 Score: 346.3 bits (887), Expect = 1.1e-91
Identity = 153/217 (70.51%), Postives = 188/217 (86.64%), Query Frame = 1

Query: 402 VVKGITFKNTYNVPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYT 461
           VV+GITF+NTYN  GSV R+EDI PA+AALI+GDK IFHKCGF+GLQDTLWDG GRH +T
Sbjct: 43  VVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFT 102

Query: 462 ECYIEGVIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCS 521
           +CYIEGVIDVISG GQSIY+ C INIP++ YAP++  G+ITAQGKE+ S+++GFVFI C 
Sbjct: 103 QCYIEGVIDVISGFGQSIYKECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCI 162

Query: 522 VIGSGTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGAD 581
           V GSG V+LGRAYRPFSTVI++  FL +CI+PAGW+ W+QVGHE++LTY ETRCIGPGAD
Sbjct: 163 VKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGAD 222

Query: 582 TSKRVPWLKKLSIDEIRRFTSISFVDQEG-WTSKLPL 618
           TS RVPWLK+L + +IRRFT IS++D +G WTS+LP+
Sbjct: 223 TSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLPV 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME55_ARATH6.0e-6942.35Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1[more]
PME66_ARATH5.1e-6844.52Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2[more]
PME29_ARATH3.3e-6742.62Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1[more]
PME10_ARATH1.4e-6543.97Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1[more]
PME52_ARATH6.2e-5848.20Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
B9RNC0_RICCO1.8e-14443.52Pectinesterase OS=Ricinus communis GN=RCOM_1346520 PE=4 SV=1[more]
A0A0A0LB12_CUCSA7.5e-11158.63Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1[more]
A0A151U2J1_CAJCA1.2e-8436.27Pectinesterase (Fragment) OS=Cajanus cajan GN=KK1_006073 PE=4 SV=1[more]
A0A061DSC1_THECC3.6e-8147.52Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1[more]
V4VWS4_9ROSI2.4e-8047.32Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G18990.13.4e-7042.35 Pectin lyase-like superfamily protein[more]
AT2G47280.12.8e-6944.52 Pectin lyase-like superfamily protein[more]
AT3G24130.11.8e-6842.62 Pectin lyase-like superfamily protein[more]
AT2G19150.17.7e-6743.97 Pectin lyase-like superfamily protein[more]
AT5G26810.13.5e-5948.20 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|1009147929|ref|XP_015891671.1|7.1e-15542.57PREDICTED: uncharacterized protein LOC107426085 [Ziziphus jujuba][more]
gi|802700695|ref|XP_012083804.1|1.3e-14542.69PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase 30 [Jatropha curcas][more]
gi|223545739|gb|EEF47243.1|2.5e-14443.52pectinesterase, putative [Ricinus communis][more]
gi|700203001|gb|KGN58134.1|1.1e-11058.63hypothetical protein Csa_3G536660 [Cucumis sativus][more]
gi|659093698|ref|XP_008447669.1|1.1e-9170.51PREDICTED: putative pectinesterase 52 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g16160.1Cp4.1LG02g16160.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 399..607
score: 2.2E-39coord: 88..302
score: 8.6
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 316..614
score: 1.36E-58coord: 38..304
score: 1.41
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 316..613
score: 2.4E-75coord: 39..305
score: 3.0
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 307..618
score: 6.6E
NoneNo IPR availablePANTHERPTHR31321:SF32PECTINESTERASE 52-RELATEDcoord: 307..618
score: 6.6E

The following gene(s) are paralogous to this gene:

None