Cucsa.300590 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.300590
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Pectinesterase
Location	scaffold02931 : 124061 .. 125888 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCACCACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAATTTCTCCTGGCATATATACGTACGGTATACGATCTTATTTCCCGTTTATTAAAATATCATTTTAGTTCCCTTGTTTGATTTCATTTTATTTTCATCAAGTTTAGATTTAGTTTTTCTTTTTCTTTTTCCATACCTACTAAAGTCTATCACTGTTTGATTTCTATCAGCATTTATTAGTGATAGGTGATATTTATAGAAACATGAATAGTCTAATTAGACATGCATAGAACCTTTATATTGAGGTATATACTAAAAGAGACACTACGTGACAATAATAACTTTTGAGCATGTGATACATCAACCAAAGTTTTTTAAGCATATAAATGTATCAATTCATATGTTATGAAGTTTCTTTTACTATAAGAATGATATTATATTTTTAAGAAAGAATATTTTAATAAATGTGTAATCATCATGTCGTGTCTTAGATTTTTAAATAATGGCGTGCTGTGGTGTCCCTATTGTGCCATATCCATGTCTCAAATTTGAATTTGTGCTATTAGGATCGGATGACAGTAGTCTTGTTTATCGTCTATCAATGATATTTTGTTATATATACTTGGAATCAATTAAAACAATCATTACACATGGTATATTTTAACAGGGAAAAAGTGACGATACCCTCAGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGGTATATACATATAACTGTTTAATTTCCCTAACTTTCAAATAATGTATATATTTTCTTTGTTTTATGTCTATTATCTGTGATCCAAAATTGGCAGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTAATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGTTAACTTCTAAATTAATTATAAGCTTACATGTGTGGTTGTATATGTGTGAGAGCTTGGAAGTGAAATTGAAGTGTTGAGTTTTTCTTTTCTGTTTGTTTGTTTGTTGTGTTTAACGCAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGTAATAATTAACTCTTCTTTTCATCTTTTTCATTTCATTTTATATAGTATATTATTAAAGTATCATTATTATCATTATTATGATTATTATCATCATTTGTTTGTAGGAGTGATTTGACGTATTCAGAGACCCGTTGTATTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGATTCCTCTTTTATCCTAA

mRNA sequence

TTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCACCACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAATTTCTCCTGGCATATATACGGAAAAAGTGACGATACCCTCAGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTAATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGAGTGATTTGACGTATTCAGAGACCCGTTGTATTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGATTCCTCTTTTATCCTAA

Coding sequence (CDS)

TTGCATACACTATTGTTGTTGATAAACTTGGGAAAGGTAACTTCACCACAATCCAAAGTGCCATTAATTCTGTCCCTTCCCAAAGTACCAAATGGATTAGAATTCAAATTTCTCCTGGCATATATACGGAAAAAGTGACGATACCCTCAGAAAAATCATGCATATTTCTAGATGGAAGTGGGCTTCAAGTGACTGAAATTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTGCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAGACAAAGTAATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGAATGTGTGATAAACATACCAGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAGGCTGGGCATGAGAGTGATTTGACGTATTCAGAGACCCGTTGTATTGGGCCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGGTTAATAGATTGGATGCATTTCACATAAGAAGCTTCACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGATTCCTCTTTTATCCTAA

Protein sequence

AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS*

Homology

[Close]

BLAST of Cucsa.300590 vs. Swiss-Prot
Match: PME55_ARATH (Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.5e-76
Identity = 139/305 (45.57%), Postives = 196/305 (64.26%), Query Frame = 1


Query: 2   YTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGL 61
           + ++VD+ G GNFTTIQ AI+SVP  +T W  I +  G+Y EK+TIP +K  I + G+G 
Sbjct: 27  FEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGK 86

Query: 62  QVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQ 121
           + T + W+DH + A SPTF   A N VV+ ITF N+YN  + G + +   + PA+AA I 
Sbjct: 87  RSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV-PAVAAFIG 146

Query: 122 GDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYA 181
           GDK  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      
Sbjct: 147 GDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLG 206

Query: 182 PLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINP 241
           P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LGRA+RP+S VIF+   L   ++P
Sbjct: 207 PGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 266

Query: 242 AGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWT 301
            GW  W   G+E  LTY+E  C G G++TS R  WV +L A  ++   D+S+I+ +G W 
Sbjct: 267 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFIN-RGGWV 326

Query: 302 SRIPL 305
             +P+
Sbjct: 327 EDLPI 328

[Close]

BLAST of Cucsa.300590 vs. Swiss-Prot
Match: PME66_ARATH (Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2)

HSP 1 Score: 287.0 bits (733), Expect = 2.5e-76
Identity = 143/304 (47.04%), Postives = 192/304 (63.16%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           AYTI VD  G GNFTT+QSAI+S+   +  WIR+    GIY EKVTIP EK  I+L G G
Sbjct: 32  AYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQGKG 91

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG 120
           ++ T I ++DH+ T  S TFTA A ++V+ GITF+NTYN   +   + +I PA+AA + G
Sbjct: 92  IEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARMLG 151

Query: 121 DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP 180
           D+ +     F+GLQDTL+DG GRH + +C I G ID I G+GQS++KEC +N+ +  YAP
Sbjct: 152 DRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIYAP 211

Query: 181 LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA 240
               G ITA  + +P++  GFVF  C V G G   LGRA+   + VIF    L   + P 
Sbjct: 212 DNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVLPI 271

Query: 241 GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS 300
           GW++W   G+E DLT+ E  C G GADTS RVPW+ +L    +  F  +S+ID  G W S
Sbjct: 272 GWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDG-WIS 331

Query: 301 RIPL 305
           R P+
Sbjct: 332 RFPI 332

[Close]

BLAST of Cucsa.300590 vs. Swiss-Prot
Match: PME29_ARATH (Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 6.8e-74
Identity = 139/303 (45.87%), Postives = 194/303 (64.03%), Query Frame = 1


Query: 4   IVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQV 63
           + VD+ G GNFTTIQ AI+SVP  +  W  I +  G+Y EK+ IP EK  I L G+G ++
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 64  TEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQGD 123
           T + W+DH + A SPTF+  A N VV+ ITF N+YN  ++G  M +    PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGK-MNKNPRTPAVAALIGGD 153

Query: 124 KVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPL 183
           K  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      P 
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPG 213

Query: 184 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 243
           L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLGR +R +S VIF+   L   + P G
Sbjct: 214 L-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEG 273

Query: 244 WNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSR 303
           W++W   GHE+ L ++E  C G GA+   RV WV +L    I++  D+S+I+ +G W   
Sbjct: 274 WDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFIN-RGGWVED 333

Query: 304 IPL 305
           +P+
Sbjct: 334 LPI 333

[Close]

BLAST of Cucsa.300590 vs. Swiss-Prot
Match: PME10_ARATH (Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.8e-70
Identity = 135/304 (44.41%), Postives = 185/304 (60.86%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           A TI+V+      F T+QSAI+S+P Q+  WIRI IS GIY+EKVTIP  K  I++ G G
Sbjct: 38  AKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGGG 97

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG 120
           ++ T I + DH+ T  S TFT+   N+++ GITF+N YN   S       KPA+AA++ G
Sbjct: 98  IEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMMLG 157

Query: 121 DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP 180
           DK       F G QDTL+D  GRH + +C I G ID I G  QSI++ C + + V  Y P
Sbjct: 158 DKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIYPP 217

Query: 181 LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA 240
               G ITAQG+++P +  GFVF  C V GSG   LGRA++ +S VIF+       I P 
Sbjct: 218 NEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNILPI 277

Query: 241 GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS 300
           GW++W   G E  +T+ E  C G GADTS RVPW+ +     +  FT++++ID +G W S
Sbjct: 278 GWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEG-WLS 337

Query: 301 RIPL 305
           R+P+
Sbjct: 338 RLPI 337

[Close]

BLAST of Cucsa.300590 vs. Swiss-Prot
Match: PME52_ARATH (Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 6.6e-61
Identity = 118/250 (47.20%), Postives = 156/250 (62.40%), Query Frame = 1


Query: 58  GSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRRE----DIKPA 117
           G G +VT I +N H  T  S TFT+   ++VV+ ++  NTYN   S+ +      DIKPA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 118 LAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 177
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G GQS+Y++C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 178 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFL 237
              A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LGRAY PFS VIF    L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 238 PSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYID 297
            S ++P GW SW   G+E   TY+E  C G G+D S RVPW+++L +F+ +    IS   
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 298 PQGYWTSRIP 304
            Q  W S IP
Sbjct: 282 DQDQWISNIP 291

[Close]

BLAST of Cucsa.300590 vs. TrEMBL
Match: A0A0A0LB12_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 9.3e-179
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG
Sbjct: 43  AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 102

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG 120
           LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG
Sbjct: 103 LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG 162

Query: 121 DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP 180
           DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP
Sbjct: 163 DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP 222

Query: 181 LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA 240
           LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA
Sbjct: 223 LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA 282

Query: 241 GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS 300
           GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS
Sbjct: 283 GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS 342

Query: 301 RIPLLS 307
           RIPLLS
Sbjct: 343 RIPLLS 348

[Close]

BLAST of Cucsa.300590 vs. TrEMBL
Match: V4VWS4_9ROSI (Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 9.2e-86
Identity = 159/306 (51.96%), Postives = 201/306 (65.69%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           AYTI V + G GNFT IQ AIN +P  + +WIRIQISP IY EKVTIP  K CIFL+G+ 
Sbjct: 14  AYTISVSRSGGGNFTKIQDAINFIPVNNDQWIRIQISPEIYQEKVTIPQNKPCIFLEGAS 73

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR---REDIKPALAAL 120
            + T+I W DH+TT +SPTFT+ ++N+V +GI F+NTYN   S  +   R ++K ALAA 
Sbjct: 74  SRTTKIQWGDHDTTISSPTFTSLSENVVAKGILFQNTYNVPPSYRQNQERMEVKQALAAR 133

Query: 121 IQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNA 180
           I GDK  F+ CGF GLQDTL+D  GRH F QCYIEG ID I G GQSIY  C +N+ +  
Sbjct: 134 ISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQCYIEGAIDFIFGNGQSIYHNCAVNVTLKE 193

Query: 181 YAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCI 240
           Y P  + G+ITAQG+ + ++ SGFVF  C   GSG  +LGRAY  FS VI     L   +
Sbjct: 194 YLPEKDYGYITAQGRNSTDDPSGFVFTSCQFLGSGKAYLGRAYGAFSRVIIVNSVLTDMV 253

Query: 241 NPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGY 300
            P GWN+W    HE ++ Y+E RC G GADTS RV W   L    ++ FTD S+ID  G 
Sbjct: 254 EPQGWNAWNHVEHEENIEYAEARCRGAGADTSKRVSWEKHLSVDEVKKFTDSSFIDHDG- 313

Query: 301 WTSRIP 304
           W +++P
Sbjct: 314 WIAKLP 318

[Close]

BLAST of Cucsa.300590 vs. TrEMBL
Match: A0A067HG98_CITSI (Pectinesterase OS=Citrus sinensis GN=CISIN_1g043974mg PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 9.2e-86
Identity = 159/306 (51.96%), Postives = 201/306 (65.69%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           AYTI V + G GNFT IQ AIN +P  + +WIRIQISP IY EKVTIP  K CIFL+G+ 
Sbjct: 37  AYTISVSRSGGGNFTKIQDAINFIPVNNDQWIRIQISPEIYQEKVTIPQNKPCIFLEGAS 96

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR---REDIKPALAAL 120
            + T+I W DH+TT +SPTFT+ ++N+V +GI F+NTYN   S  +   R ++K ALAA 
Sbjct: 97  SRTTKIQWGDHDTTISSPTFTSLSENVVAKGILFQNTYNVPPSYRQNQERMEVKQALAAR 156

Query: 121 IQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNA 180
           I GDK  F+ CGF GLQDTL+D  GRH F QCYIEG ID I G GQSIY  C +N+ +  
Sbjct: 157 ISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQCYIEGAIDFIFGNGQSIYHNCAVNVTLKE 216

Query: 181 YAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCI 240
           Y P  + G+ITAQG+ + ++ SGFVF  C   GSG  +LGRAY  FS VI     L   +
Sbjct: 217 YLPEKDYGYITAQGRNSTDDPSGFVFTSCQFLGSGKAYLGRAYGAFSRVIIVNSVLTDMV 276

Query: 241 NPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGY 300
            P GWN+W    HE ++ Y+E RC G GADTS RV W   L    ++ FTD S+ID  G 
Sbjct: 277 EPQGWNAWNHVEHEENIEYAEARCRGAGADTSKRVSWEKHLSVDEVKKFTDSSFIDHDG- 336

Query: 301 WTSRIP 304
           W +++P
Sbjct: 337 WIAKLP 341

[Close]

BLAST of Cucsa.300590 vs. TrEMBL
Match: A0A061DSC1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 9.2e-86
Identity = 154/306 (50.33%), Postives = 206/306 (67.32%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           A+TI VD+ GKG+F TIQ+AI+S+PS + KWIRI ISPG + EKVTIP+ K CIFL+G+G
Sbjct: 53  AHTIRVDQSGKGDFKTIQNAIDSIPSNNDKWIRILISPGHFREKVTIPANKPCIFLEGAG 112

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG 120
            ++T I W DH  T  S TFT+S  N+V +GI+F+N YN       ++ ++PALA  I G
Sbjct: 113 SKLTSIEWGDHMDTCDSATFTSSPDNIVAKGISFKNIYNIPSGPDAKKKVEPALAVRIYG 172

Query: 121 DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP 180
           DK  F+ C F GLQDTLWD  GRH F  CYIEG +D I G GQSIY++C IN+    YAP
Sbjct: 173 DKSAFYNCAFFGLQDTLWDVQGRHYFYNCYIEGAVDFIFGSGQSIYEKCEINLTAGKYAP 232

Query: 181 LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA 240
               G+ITAQG+ + ++ SGFVF  C+  G G  +LGRAY  +S VI +   L   I  +
Sbjct: 233 EYPNGYITAQGRNSSDDPSGFVFKYCVFTGYGKTYLGRAYGAYSRVIIYKSVLSDAILAS 292

Query: 241 GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS 300
           GW++W    HE +L Y E  C GPGA+TS RVPW+ +L A  ++ F ++SYID +G W +
Sbjct: 293 GWDAWRYVHHEGNLIYVEADCKGPGANTSKRVPWLKKLGASQLKKFLNLSYIDKEG-WIA 352

Query: 301 RIPLLS 307
           ++P +S
Sbjct: 353 KLPKVS 357

[Close]

BLAST of Cucsa.300590 vs. TrEMBL
Match: B9RNC0_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1346520 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-84
Identity = 161/309 (52.10%), Postives = 213/309 (68.93%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           A TI VD+ G G+FT++QSAI+S+P  +T+WI IQISPG Y EKVTIP +K CIFL+G+G
Sbjct: 35  ARTITVDQSGNGDFTSVQSAIDSIPEMNTQWIHIQISPGKYREKVTIPVKKPCIFLEGAG 94

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR--REDI--KPALAA 120
           +++T I W DHE T+ S TFT+   N+V +GITF+NTYN   + +    E I  + A++A
Sbjct: 95  IRLTSIEWGDHEATSTSATFTSYPDNIVAKGITFKNTYNLDITKINWWGEKIIWRQAVSA 154

Query: 121 LIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVN 180
            I+G++  F+KC F+G QDTLWD  GRH F+ CYIEG ID I G  QSIY+ CVI++ + 
Sbjct: 155 RIKGEQCAFYKCAFLGTQDTLWDEKGRHYFSNCYIEGAIDFIFGKAQSIYEGCVISVNIG 214

Query: 181 AYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSC 240
            Y P L +G ITAQ KE P  SSGFVF  C+V G+G  FLGRA+ P+STVIF+   L   
Sbjct: 215 KYPPGL-QGCITAQKKEWPQHSSGFVFKNCVVSGTGKAFLGRAWGPYSTVIFYNSTLSDV 274

Query: 241 INPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQG 300
           I   GWN+W    HE++ TY+E    G GADTS RVPW  +L+A  +R F D+S++D  G
Sbjct: 275 IVSEGWNAWNYVHHEANFTYAEANNRGVGADTSKRVPWEKKLNADQLRRFLDLSFVDGGG 334

Query: 301 YWTSRIPLL 306
            W ++IP L
Sbjct: 335 -WLAKIPKL 341

[Close]

BLAST of Cucsa.300590 vs. TAIR10
Match: AT5G18990.1 (AT5G18990.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 287.7 bits (735), Expect = 8.3e-78
Identity = 139/305 (45.57%), Postives = 196/305 (64.26%), Query Frame = 1


Query: 2   YTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGL 61
           + ++VD+ G GNFTTIQ AI+SVP  +T W  I +  G+Y EK+TIP +K  I + G+G 
Sbjct: 27  FEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGK 86

Query: 62  QVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQ 121
           + T + W+DH + A SPTF   A N VV+ ITF N+YN  + G + +   + PA+AA I 
Sbjct: 87  RSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV-PAVAAFIG 146

Query: 122 GDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYA 181
           GDK  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      
Sbjct: 147 GDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLG 206

Query: 182 PLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINP 241
           P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LGRA+RP+S VIF+   L   ++P
Sbjct: 207 PGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 266

Query: 242 AGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWT 301
            GW  W   G+E  LTY+E  C G G++TS R  WV +L A  ++   D+S+I+ +G W 
Sbjct: 267 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFIN-RGGWV 326

Query: 302 SRIPL 305
             +P+
Sbjct: 327 EDLPI 328

[Close]

BLAST of Cucsa.300590 vs. TAIR10
Match: AT2G47280.1 (AT2G47280.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 287.0 bits (733), Expect = 1.4e-77
Identity = 143/304 (47.04%), Postives = 192/304 (63.16%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           AYTI VD  G GNFTT+QSAI+S+   +  WIR+    GIY EKVTIP EK  I+L G G
Sbjct: 32  AYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQGKG 91

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG 120
           ++ T I ++DH+ T  S TFTA A ++V+ GITF+NTYN   +   + +I PA+AA + G
Sbjct: 92  IEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARMLG 151

Query: 121 DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP 180
           D+ +     F+GLQDTL+DG GRH + +C I G ID I G+GQS++KEC +N+ +  YAP
Sbjct: 152 DRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIYAP 211

Query: 181 LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA 240
               G ITA  + +P++  GFVF  C V G G   LGRA+   + VIF    L   + P 
Sbjct: 212 DNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVLPI 271

Query: 241 GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS 300
           GW++W   G+E DLT+ E  C G GADTS RVPW+ +L    +  F  +S+ID  G W S
Sbjct: 272 GWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDG-WIS 331

Query: 301 RIPL 305
           R P+
Sbjct: 332 RFPI 332

[Close]

BLAST of Cucsa.300590 vs. TAIR10
Match: AT3G24130.1 (AT3G24130.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 278.9 bits (712), Expect = 3.8e-75
Identity = 139/303 (45.87%), Postives = 194/303 (64.03%), Query Frame = 1


Query: 4   IVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQV 63
           + VD+ G GNFTTIQ AI+SVP  +  W  I +  G+Y EK+ IP EK  I L G+G ++
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 64  TEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYN--ARGSVMRREDIKPALAALIQGD 123
           T + W+DH + A SPTF+  A N VV+ ITF N+YN  ++G  M +    PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGK-MNKNPRTPAVAALIGGD 153

Query: 124 KVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPL 183
           K  F+  GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CVI +      P 
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPG 213

Query: 184 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 243
           L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLGR +R +S VIF+   L   + P G
Sbjct: 214 L-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEG 273

Query: 244 WNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSR 303
           W++W   GHE+ L ++E  C G GA+   RV WV +L    I++  D+S+I+ +G W   
Sbjct: 274 WDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFIN-RGGWVED 333

Query: 304 IPL 305
           +P+
Sbjct: 334 LPI 333

[Close]

BLAST of Cucsa.300590 vs. TAIR10
Match: AT2G19150.1 (AT2G19150.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 265.4 bits (677), Expect = 4.4e-71
Identity = 135/304 (44.41%), Postives = 185/304 (60.86%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           A TI+V+      F T+QSAI+S+P Q+  WIRI IS GIY+EKVTIP  K  I++ G G
Sbjct: 38  AKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGGG 97

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG 120
           ++ T I + DH+ T  S TFT+   N+++ GITF+N YN   S       KPA+AA++ G
Sbjct: 98  IEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMMLG 157

Query: 121 DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP 180
           DK       F G QDTL+D  GRH + +C I G ID I G  QSI++ C + + V  Y P
Sbjct: 158 DKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIYPP 217

Query: 181 LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA 240
               G ITAQG+++P +  GFVF  C V GSG   LGRA++ +S VIF+       I P 
Sbjct: 218 NEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNILPI 277

Query: 241 GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS 300
           GW++W   G E  +T+ E  C G GADTS RVPW+ +     +  FT++++ID +G W S
Sbjct: 278 GWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEG-WLS 337

Query: 301 RIPL 305
           R+P+
Sbjct: 338 RLPI 337

[Close]

BLAST of Cucsa.300590 vs. TAIR10
Match: AT5G26810.1 (AT5G26810.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 235.7 bits (600), Expect = 3.7e-62
Identity = 118/250 (47.20%), Postives = 156/250 (62.40%), Query Frame = 1


Query: 58  GSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRRE----DIKPA 117
           G G +VT I +N H  T  S TFT+   ++VV+ ++  NTYN   S+ +      DIKPA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 118 LAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 177
           +A  + GDK  F+ C F+GLQDT+WD  GRH F  CYIEG ID I G GQS+Y++C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 178 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFL 237
              A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LGRAY PFS VIF    L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 238 PSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYID 297
            S ++P GW SW   G+E   TY+E  C G G+D S RVPW+++L +F+ +    IS   
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 298 PQGYWTSRIP 304
            Q  W S IP
Sbjct: 282 DQDQWISNIP 291

[Close]

BLAST of Cucsa.300590 vs. NCBI nr
Match: gi|700203001|gb|KGN58134.1| (hypothetical protein Csa_3G536660 [Cucumis sativus])

HSP 1 Score: 634.0 bits (1634), Expect = 1.3e-178
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG
Sbjct: 43  AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 102

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG 120
           LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG
Sbjct: 103 LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQG 162

Query: 121 DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP 180
           DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP
Sbjct: 163 DKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAP 222

Query: 181 LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA 240
           LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA
Sbjct: 223 LLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPA 282

Query: 241 GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS 300
           GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS
Sbjct: 283 GWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTS 342

Query: 301 RIPLLS 307
           RIPLLS
Sbjct: 343 RIPLLS 348

[Close]

BLAST of Cucsa.300590 vs. NCBI nr
Match: gi|778688667|ref|XP_011652807.1| (PREDICTED: probable pectinesterase 55 [Cucumis sativus])

HSP 1 Score: 552.0 bits (1421), Expect = 6.7e-154
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 1


Query: 43  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 102
           EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG
Sbjct: 33  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 92

Query: 103 SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 162
           SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG
Sbjct: 93  SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 152

Query: 163 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 222
           QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP
Sbjct: 153 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 212

Query: 223 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH 282
           FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH
Sbjct: 213 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH 272

Query: 283 IRSFTDISYIDPQGYWTSRIPLLS 307
           IRSFTDISYIDPQGYWTSRIPLLS
Sbjct: 273 IRSFTDISYIDPQGYWTSRIPLLS 296

[Close]

BLAST of Cucsa.300590 vs. NCBI nr
Match: gi|659093698|ref|XP_008447669.1| (PREDICTED: putative pectinesterase 52 [Cucumis melo])

HSP 1 Score: 493.0 bits (1268), Expect = 3.7e-136
Identity = 230/260 (88.46%), Postives = 244/260 (93.85%), Query Frame = 1


Query: 47  IPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR 106
           IP +KSCIFLDGSG +VTEI WNDH TTA SPTFT+ AQNLVVQGITFRNTYNA GSV R
Sbjct: 2   IPPDKSCIFLDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTR 61

Query: 107 REDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY 166
           REDIKPA+AALIQGDK IFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY
Sbjct: 62  REDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY 121

Query: 167 KECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTV 226
           KECVINIP+NAYAPLLNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLGRAYRPFSTV
Sbjct: 122 KECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTV 181

Query: 227 IFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSF 286
           IFHLCFLPSCI+PAGWNSW Q GHES+LTYSETRCIGPGADTSSRVPW+ RLD + IR F
Sbjct: 182 IFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRF 241

Query: 287 TDISYIDPQGYWTSRIPLLS 307
           TDISYIDPQGYWTSR+P+LS
Sbjct: 242 TDISYIDPQGYWTSRLPVLS 261

[Close]

BLAST of Cucsa.300590 vs. NCBI nr
Match: gi|985469142|ref|XP_015380662.1| (PREDICTED: probable pectinesterase 55 [Citrus sinensis])

HSP 1 Score: 325.1 bits (832), Expect = 1.3e-85
Identity = 159/306 (51.96%), Postives = 201/306 (65.69%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           AYTI V + G GNFT IQ AIN +P  + +WIRIQISP IY EKVTIP  K CIFL+G+ 
Sbjct: 37  AYTISVSRSGGGNFTKIQDAINFIPVNNDQWIRIQISPEIYQEKVTIPQNKPCIFLEGAS 96

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR---REDIKPALAAL 120
            + T+I W DH+TT +SPTFT+ ++N+V +GI F+NTYN   S  +   R ++K ALAA 
Sbjct: 97  SRTTKIQWGDHDTTISSPTFTSLSENVVAKGILFQNTYNVPPSYRQNQERMEVKQALAAR 156

Query: 121 IQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNA 180
           I GDK  F+ CGF GLQDTL+D  GRH F QCYIEG ID I G GQSIY  C +N+ +  
Sbjct: 157 ISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQCYIEGAIDFIFGNGQSIYHNCAVNVTLKE 216

Query: 181 YAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCI 240
           Y P  + G+ITAQG+ + ++ SGFVF  C   GSG  +LGRAY  FS VI     L   +
Sbjct: 217 YLPEKDYGYITAQGRNSTDDPSGFVFTSCQFLGSGKAYLGRAYGAFSRVIIVNSVLTDMV 276

Query: 241 NPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGY 300
            P GWN+W    HE ++ Y+E RC G GADTS RV W   L    ++ FTD S+ID  G 
Sbjct: 277 EPQGWNAWNHVEHEENIEYAEARCRGAGADTSKRVSWEKHLSVDEVKKFTDSSFIDHDG- 336

Query: 301 WTSRIP 304
           W +++P
Sbjct: 337 WIAKLP 341

[Close]

BLAST of Cucsa.300590 vs. NCBI nr
Match: gi|567904092|ref|XP_006444534.1| (hypothetical protein CICLE_v10024124mg, partial [Citrus clementina])

HSP 1 Score: 325.1 bits (832), Expect = 1.3e-85
Identity = 159/306 (51.96%), Postives = 201/306 (65.69%), Query Frame = 1


Query: 1   AYTIVVDKLGKGNFTTIQSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSG 60
           AYTI V + G GNFT IQ AIN +P  + +WIRIQISP IY EKVTIP  K CIFL+G+ 
Sbjct: 14  AYTISVSRSGGGNFTKIQDAINFIPVNNDQWIRIQISPEIYQEKVTIPQNKPCIFLEGAS 73

Query: 61  LQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMR---REDIKPALAAL 120
            + T+I W DH+TT +SPTFT+ ++N+V +GI F+NTYN   S  +   R ++K ALAA 
Sbjct: 74  SRTTKIQWGDHDTTISSPTFTSLSENVVAKGILFQNTYNVPPSYRQNQERMEVKQALAAR 133

Query: 121 IQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNA 180
           I GDK  F+ CGF GLQDTL+D  GRH F QCYIEG ID I G GQSIY  C +N+ +  
Sbjct: 134 ISGDKSAFYNCGFAGLQDTLFDDHGRHYFHQCYIEGAIDFIFGNGQSIYHNCAVNVTLKE 193

Query: 181 YAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCI 240
           Y P  + G+ITAQG+ + ++ SGFVF  C   GSG  +LGRAY  FS VI     L   +
Sbjct: 194 YLPEKDYGYITAQGRNSTDDPSGFVFTSCQFLGSGKAYLGRAYGAFSRVIIVNSVLTDMV 253

Query: 241 NPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGY 300
            P GWN+W    HE ++ Y+E RC G GADTS RV W   L    ++ FTD S+ID  G 
Sbjct: 254 EPQGWNAWNHVEHEENIEYAEARCRGAGADTSKRVSWEKHLSVDEVKKFTDSSFIDHDG- 313

Query: 301 WTSRIP 304
           W +++P
Sbjct: 314 WIAKLP 318

The following BLAST results are available for this feature:

BLAST of Cucsa.300590 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PME55_ARATH	1.5e-76	45.57	Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1	[more]
PME66_ARATH	2.5e-76	47.04	Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2	[more]
PME29_ARATH	6.8e-74	45.87	Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1	[more]
PME10_ARATH	7.8e-70	44.41	Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1	[more]
PME52_ARATH	6.6e-61	47.20	Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2	[more]

BLAST of Cucsa.300590 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB12_CUCSA	9.3e-179	100.00	Pectinesterase OS=Cucumis sativus GN=Csa_3G536660 PE=4 SV=1	[more]
V4VWS4_9ROSI	9.2e-86	51.96	Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10024124mg PE=4 SV=1	[more]
A0A067HG98_CITSI	9.2e-86	51.96	Pectinesterase OS=Citrus sinensis GN=CISIN_1g043974mg PE=4 SV=1	[more]
A0A061DSC1_THECC	9.2e-86	50.33	Pectinesterase OS=Theobroma cacao GN=TCM_004838 PE=4 SV=1	[more]
B9RNC0_RICCO	1.3e-84	52.10	Pectinesterase OS=Ricinus communis GN=RCOM_1346520 PE=4 SV=1	[more]

BLAST of Cucsa.300590 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G18990.1	8.3e-78	45.57	Pectin lyase-like superfamily protein	[more]
AT2G47280.1	1.4e-77	47.04	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	3.8e-75	45.87	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	4.4e-71	44.41	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	3.7e-62	47.20	Pectin lyase-like superfamily protein	[more]

BLAST of Cucsa.300590 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700203001\|gb\|KGN58134.1\|	1.3e-178	100.00	hypothetical protein Csa_3G536660 [Cucumis sativus]	[more]
gi\|778688667\|ref\|XP_011652807.1\|	6.7e-154	100.00	PREDICTED: probable pectinesterase 55 [Cucumis sativus]	[more]
gi\|659093698\|ref\|XP_008447669.1\|	3.7e-136	88.46	PREDICTED: putative pectinesterase 52 [Cucumis melo]	[more]
gi\|985469142\|ref\|XP_015380662.1\|	1.3e-85	51.96	PREDICTED: probable pectinesterase 55 [Citrus sinensis]	[more]
gi\|567904092\|ref\|XP_006444534.1\|	1.3e-85	51.96	hypothetical protein CICLE_v10024124mg, partial [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000070	Pectinesterase_cat
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Cellular Component**
Term	Definition
GO:0005618	cell wall

Vocabulary: **Molecular Function**
Term	Definition
GO:0030599	pectinesterase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0042545	cell wall modification

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005618	cell wall
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.300590.1	Cucsa.300590.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 4..292 score: 2.1
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 1..298 score: 8.64
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 2..299 score: 1.1
None	No IPR available	PANTHER	PTHR31321	FAMILY NOT NAMED	coord: 3..305 score: 3.0E
None	No IPR available	PANTHER	PTHR31321:SF32	PECTINESTERASE 52-RELATED	coord: 3..305 score: 3.0E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.300590	Cla017349	Watermelon (97103) v1	cgywmB546
Cucsa.300590	Csa3G536660	Cucumber (Chinese Long) v2	cgycuB428
Cucsa.300590	MELO3C012935	Melon (DHL92) v3.5.1	cgymeB494
Cucsa.300590	ClCG10G011540	Watermelon (Charleston Gray)	cgywcgB518
Cucsa.300590	CSPI03G25040	Wild cucumber (PI 183967)	cgycpiB451
Cucsa.300590	CmaCh01G002810	Cucurbita maxima (Rimu)	cgycmaB0829
Cucsa.300590	CmoCh01G002890	Cucurbita moschata (Rifu)	cgycmoB0822
Cucsa.300590	Lsi03G011210	Bottle gourd (USVL1VR-Ls)	cgylsiB485
Cucsa.300590	Cp4.1LG02g16160	Cucurbita pepo (Zucchini)	cgycpeB0785
Cucsa.300590	MELO3C012935.2	Melon (DHL92) v3.6.1	cgymedB493
Cucsa.300590	CsaV3_3G026620	Cucumber (Chinese Long) v3	cgycucB464
Cucsa.300590	Cla97C10G194370	Watermelon (97103) v2	cgywmbB519
Cucsa.300590	Bhi11G001077	Wax gourd	cgywgoB600

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.300590	Wax gourd	cgywgoB601