Lsi03G006560 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi03G006560
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionRING/U-box superfamily protein
Locationchr03 : 8300521 .. 8301750 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTAAGAAGAAATTATATATATATCCCTGCAACCGCTCAAGTTAATATATTATAGATTTAAATTATAACTTAATAAACAAAAAAAATTAAATATCTATATATATAATATAATAAATCAAAGAAAATTTTGTAATTTTGTATTAAAAGAAGTTAGAATGGTGAAATAATTAACGAACGCATTTGAAAAGATAGATTTTTTTTTTATCTAATATCTTTCATTCCTATTCCAATTACGAAAACATGATCCAAAGATAAAAGATATAATTGTCTTCAAAAAAAGATAAAAGATATAATTTCTTTCTCCAAATATATATATAAATTAATTAAGTAATGATATCTTGGTTTTCTAATAGAAGGGGATTTCCCATAGGCTTAAGGAAATCGGCAAGGTCTCTCTTATAAATGCCTCACATTCTATTATCAAATTTTCACAACTTTTCCCATTAATAACTCTCATCATTTAAATATTAATAATGGCGGCACAAATGTTTGAAATTTGTAGGAATGATCCTTCCTACATTGGTATGTCACTCACAATTAAAGACATATTCAAACTCGACAAAATTCATCAACATTTATTAAACAAATCGATCTGAATTTCAAAGTCCAACTCAAATGTAGTCTCTACCAAATATATGAAGAAGGCAATAGGAATACATCATCTTTCCTCGGTGAAGACATGTCTTCACAACATCCCTTTGCTACATACCAAATTCTTGTCTCCATCTTAAAACACGGCGACAAAACTTTGGAGCGACATCTATTGCAGCAGTTTCGGAGGTATAGAGACATCATCAATATCAAACTTGTGGCTAATGAAATAATAAAATACTGGGTTAGAAAAGTCGAAGAAGAAGAGGAGGATAATTTTAGGGTGTTTGGAAAGGTTTATCCTCTCGAAATTACGATAGAACTGTTGCTTTTTGAGAGAGTTGACAATGACACGATAGAAGTACCGTTAATGATGGTTCCAGCCAATAATAGCGCAATGGAGAGTATGTTAAAGAGAATGGAGATCGAAGAGATAATGAAATTAAGTGATGATGAAAGCATTAATTGCGTAATTTGTATGGAGGAAATTAGTAGAGAAGAGGAAGGAAAAAGTGGGACATTTGTGTTACAAATGCCTTGCTTGCATATGTTTCATGAAGAATGTATAAAGAAGTGGTCGAAGAGTAGTCATTATTGTCCAACTTGTAGATTTTCAATGCCTACAAATGATTGA

mRNA sequence

ATGGATTTAGAATGGGGATTTCCCATAGGCTTAAGGAAATCGGCAAGACATATTCAAACTCGACAAAATTCATCAACATTTATTAAACAAATCGATCTGAATTTCAAAGTCCAACTCAAATGTAGTCTCTACCAAATATATGAAGAAGGCAATAGGAATACATCATCTTTCCTCGGTGAAGACATGTCTTCACAACATCCCTTTGCTACATACCAAATTCTTGTCTCCATCTTAAAACACGGCGACAAAACTTTGGAGCGACATCTATTGCAGCAGTTTCGGAGGTATAGAGACATCATCAATATCAAACTTGTGGCTAATGAAATAATAAAATACTGGGTTAGAAAAGTCGAAGAAGAAGAGGAGGATAATTTTAGGGTGTTTGGAAAGGTTTATCCTCTCGAAATTACGATAGAACTGTTGCTTTTTGAGAGAGTTGACAATGACACGATAGAAGTACCGTTAATGATGGTTCCAGCCAATAATAGCGCAATGGAGAGTATGTTAAAGAGAATGGAGATCGAAGAGATAATGAAATTAAGTGATGATGAAAGCATTAATTGCGTAATTTGTATGGAGGAAATTAGTAGAGAAGAGGAAGGAAAAAGTGGGACATTTGTGTTACAAATGCCTTGCTTGCATATGTTTCATGAAGAATGTATAAAGAAGTGGTCGAAGAGTAGTCATTATTGTCCAACTTGTAGATTTTCAATGCCTACAAATGATTGA

Coding sequence (CDS)

ATGGATTTAGAATGGGGATTTCCCATAGGCTTAAGGAAATCGGCAAGACATATTCAAACTCGACAAAATTCATCAACATTTATTAAACAAATCGATCTGAATTTCAAAGTCCAACTCAAATGTAGTCTCTACCAAATATATGAAGAAGGCAATAGGAATACATCATCTTTCCTCGGTGAAGACATGTCTTCACAACATCCCTTTGCTACATACCAAATTCTTGTCTCCATCTTAAAACACGGCGACAAAACTTTGGAGCGACATCTATTGCAGCAGTTTCGGAGGTATAGAGACATCATCAATATCAAACTTGTGGCTAATGAAATAATAAAATACTGGGTTAGAAAAGTCGAAGAAGAAGAGGAGGATAATTTTAGGGTGTTTGGAAAGGTTTATCCTCTCGAAATTACGATAGAACTGTTGCTTTTTGAGAGAGTTGACAATGACACGATAGAAGTACCGTTAATGATGGTTCCAGCCAATAATAGCGCAATGGAGAGTATGTTAAAGAGAATGGAGATCGAAGAGATAATGAAATTAAGTGATGATGAAAGCATTAATTGCGTAATTTGTATGGAGGAAATTAGTAGAGAAGAGGAAGGAAAAAGTGGGACATTTGTGTTACAAATGCCTTGCTTGCATATGTTTCATGAAGAATGTATAAAGAAGTGGTCGAAGAGTAGTCATTATTGTCCAACTTGTAGATTTTCAATGCCTACAAATGATTGA

Protein sequence

MDLEWGFPIGLRKSARHIQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLEITIELLLFERVDNDTIEVPLMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND
Homology
[Close]
BLAST of Lsi03G006560 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.7e-09
Identity = 29/84 (34.52%), Postives = 50/84 (59.52%), Query Frame = 1 

Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA+ SA++++      ++++K    E   C +CM      +E + G+ V QMPC H+FH+
Sbjct: 190 PASKSAIDALPTVKVTKDMLK---SEMNQCAVCM------DEFEDGSDVKQMPCKHVFHQ 249

Query: 219 ECIKKWSKSSHYCPTCRFSMPTND 243
           +C+  W +  + CP CRF +PT+D
Sbjct: 250 DCLLPWLELHNSCPVCRFELPTDD 264

[Close]
BLAST of Lsi03G006560 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-07
Identity = 28/85 (32.94%), Postives = 48/85 (56.47%), Query Frame = 1 

Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESIN-CVICMEEISREEEGKSGTFVLQMPCLHMFH 218
           PA+ SA+E++     +  I K + +   N C +CM      ++ + GT   QMPC H++H
Sbjct: 198 PASKSAIEAL----PLVNITKSNLNSEFNQCAVCM------DDFEEGTEAKQMPCKHLYH 257

Query: 219 EECIKKWSKSSHYCPTCRFSMPTND 243
           ++C+  W +  + CP CR  +PT+D
Sbjct: 258 KDCLLPWLELHNSCPVCRHELPTDD 272

[Close]
BLAST of Lsi03G006560 vs. Swiss-Prot
Match: YKW3_SCHPO (Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAP32A8.03c PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.6e-06
Identity = 27/78 (34.62%), Postives = 41/78 (52.56%), Query Frame = 1 

Query: 164 AMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKK 223
           A E ++ +M++++  K   DE   C ICME     ++      V+Q+PC H FHE CIK 
Sbjct: 372 APEDVIAKMKVQKPPKELIDEEGECTICMEMFKINDD------VIQLPCKHYFHENCIKP 431

Query: 224 WSKSSHYCPTCRFSMPTN 242
           W + +  C  CR  +  N
Sbjct: 432 WLRVNGTCAICRAPVDPN 443

[Close]
BLAST of Lsi03G006560 vs. Swiss-Prot
Match: SIS3_ARATH (E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana GN=SIS3 PE=2 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 6.3e-06
Identity = 30/92 (32.61%), Postives = 46/92 (50.00%), Query Frame = 1 

Query: 147 DNDTIEVPLMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTF 206
           D  T    L + PA   A+E++++ +    +  + DD    C+IC+EE     E      
Sbjct: 195 DAATYHPGLYLTPAQTEAVEALIQELPKFRLKAVPDDCG-ECLICLEEFHIGHE------ 254

Query: 207 VLQMPCLHMFHEECIKKWSKSSHYCPTCRFSM 239
           V  +PC H FH ECI +W + +  CP CR S+
Sbjct: 255 VRGLPCAHNFHVECIDQWLRLNVKCPRCRCSV 279

[Close]
BLAST of Lsi03G006560 vs. TrEMBL
Match: A0A0A0K2A5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G068580 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.2e-73
Identity = 145/240 (60.42%), Postives = 181/240 (75.42%), Query Frame = 1 

Query: 8   PIGLRKSARHIQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHP 67
           P+  R+    I+TR+NS TF KQIDLNF+VQLKC  +QI EE   NT++ + E + SQHP
Sbjct: 25  PVQRRRPQTQIRTRRNSPTFFKQIDLNFEVQLKCQHFQIREEETSNTTTLIDEHIVSQHP 84

Query: 68  FATYQILVSILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEED---- 127
           FAT+++ +SIL+HGDKTL+  L ++F  YRDIINI+ + +EIIKYWV KVEEEEED    
Sbjct: 85  FATFRLPISILEHGDKTLKLLLFREFHMYRDIINIEHLVDEIIKYWVTKVEEEEEDQENS 144

Query: 128 -NFRVFGKVYPLEITIELLLFERVD-NDTIEVPLMMVPANNSAMESMLKRMEIEEIMKLS 187
            + RVF K+Y LEIT+ELL+F+ V   D     LMMVP ++SAMESMLKR+E EEIMKL 
Sbjct: 145 TSSRVFKKIYRLEITLELLIFQIVRVMDYQPQVLMMVPTSDSAMESMLKRVENEEIMKLG 204

Query: 188 DDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTN 242
           DD+ I+CVIC+EEI +EE+G  G  VLQMPCLH+FH ECI KW  +SHYCPTCRF MP N
Sbjct: 205 DDDCIHCVICLEEIGKEEKGSEGV-VLQMPCLHVFHGECINKWLNTSHYCPTCRFPMPIN 263

[Close]
BLAST of Lsi03G006560 vs. TrEMBL
Match: A0A0A0K2A1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067550 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 8.1e-69
Identity = 134/231 (58.01%), Postives = 179/231 (77.49%), Query Frame = 1 

Query: 18  IQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSI 77
           IQTR NS T +KQIDLNFKV++KC  +Q  EE     ++FL E + +  PF   Q+ VSI
Sbjct: 25  IQTRSNSPTSVKQIDLNFKVEVKCQHFQAGEEDTTFATTFLSEHIIAHQPFPALQLPVSI 84

Query: 78  LKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLEIT 137
            KHGDKTL+R L Q+F+ +R IINI+LV ++IIK+WV+KVE+++E+N  VF ++YPLEIT
Sbjct: 85  FKHGDKTLKRLLFQKFQMFRAIINIELVTDQIIKHWVKKVEDDKENNSGVFEEIYPLEIT 144

Query: 138 IELLLFERVD-----NDTIEVP-LMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVIC 197
           IE+L+ + +D      +TI+ P + MVP ++SAM+SMLKR+E EEIMK+ D +SI+CVIC
Sbjct: 145 IEVLVLQMIDAVDQPQETIDQPQVRMVPTSDSAMKSMLKRVENEEIMKVGDYKSISCVIC 204

Query: 198 MEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           +E+IS+EE+G  G  VLQMPCLHMFHEECI+KW K+SHYCPTCRFSM  N+
Sbjct: 205 LEKISKEEKGSEGV-VLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMTINN 254

[Close]
BLAST of Lsi03G006560 vs. TrEMBL
Match: E5GCQ3_CUCME (Hypothetical zinc finger protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 4.0e-68
Identity = 139/228 (60.96%), Postives = 179/228 (78.51%), Query Frame = 1 

Query: 23  NSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSILKHGD 82
           +S T ++QI L FKVQLKC  +Q++E GN ++++ + E + SQH FAT+Q+ +SI KHGD
Sbjct: 40  SSPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHGD 99

Query: 83  KTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEE--EDNFRV-FGKVYPLEITIE 142
           KTL+R L ++F+ Y DIINI+L+ANEIIK+WVRKVEEEE  E+N  V F K+YPLEI I 
Sbjct: 100 KTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIR 159

Query: 143 LLLFERVDN-----DTIEVPLMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICMEE 202
           LL+ + +         I+ PL MVP ++SA+ESMLKR+E EEIMK  DDESINCV+C+EE
Sbjct: 160 LLVSKMIRAVQPRLAMIDQPL-MVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEE 219

Query: 203 ISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           IS+EE+G S T VLQMPCLHMFHEECI+KW K+SHYCPTCRFSMP ++
Sbjct: 220 ISKEEKG-SETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]
BLAST of Lsi03G006560 vs. TrEMBL
Match: A0A0A0K7L0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067560 PE=4 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.7e-66
Identity = 137/236 (58.05%), Postives = 177/236 (75.00%), Query Frame = 1 

Query: 18  IQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNT--SSFLGEDMSSQHPFATYQILV 77
           I+T  NS T +KQIDLNFKV++KC  Y    EG  +T  ++ L E +SS HP  T Q+ V
Sbjct: 36  IRTPPNSPTLVKQIDLNFKVEVKCQHYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPV 95

Query: 78  SILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLE 137
           SI KHGDKTL+R L ++F+ YR IINI+LV +EIIK+WV+KV+EE   + +VF ++YPLE
Sbjct: 96  SIFKHGDKTLKRLLFREFQIYRAIINIELVVDEIIKHWVKKVDEENSTS-QVFEEIYPLE 155

Query: 138 ITIELLLFERVDNDTIEVP---------LMMVPANNSAMESMLKRMEIEEIMKLSDDESI 197
           ITIELL+++ +    I+ P         +MMVP + SAMESMLK++E EEI+K  D++SI
Sbjct: 156 ITIELLVYQMI--HAIDQPHEIMMDQPQVMMVPTSESAMESMLKKVENEEIVKFGDNKSI 215

Query: 198 NCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           NCVIC+EEIS+E++G S   VLQMPCLHMFHEECI+KW K SHYCPTCRFSMPTN+
Sbjct: 216 NCVICLEEISKEKKG-SEAVVLQMPCLHMFHEECIRKWLKRSHYCPTCRFSMPTNN 267

[Close]
BLAST of Lsi03G006560 vs. TrEMBL
Match: A0A0A0K493_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067540 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.4e-65
Identity = 129/230 (56.09%), Postives = 175/230 (76.09%), Query Frame = 1 

Query: 19  QTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSIL 78
           QT  NS TF+KQI+L  KV++KC  +Q  EEG+   S+ + E + S HPF T+Q+ VS+ 
Sbjct: 36  QTPHNSPTFVKQINLKLKVEVKCQHFQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVF 95

Query: 79  KHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLEITI 138
           +HG++TL+R L QQF+ +R IINI+LVA+EII++WV+K E+ E ++  +  ++YPLEITI
Sbjct: 96  RHGNRTLKRLLFQQFQIFRGIINIQLVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITI 155

Query: 139 ELLLFERV-----DNDTIEVP-LMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICM 198
           ELLL   +       +TI+ P + MVPA+ SAMESMLKR+E EEI+K+ +D+SINCVIC+
Sbjct: 156 ELLLLRMIHAIDQPEETIDQPQVTMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICL 215

Query: 199 EEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           EEIS++ +G  G  VLQMPCLHMFHEECI+ W K+SHYCPTCRFSMP N+
Sbjct: 216 EEISKKMKGSEGV-VLQMPCLHMFHEECIRTWLKTSHYCPTCRFSMPINN 264

[Close]
BLAST of Lsi03G006560 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-10
Identity = 29/84 (34.52%), Postives = 50/84 (59.52%), Query Frame = 1 

Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA+ SA++++      ++++K    E   C +CM      +E + G+ V QMPC H+FH+
Sbjct: 190 PASKSAIDALPTVKVTKDMLK---SEMNQCAVCM------DEFEDGSDVKQMPCKHVFHQ 249

Query: 219 ECIKKWSKSSHYCPTCRFSMPTND 243
           +C+  W +  + CP CRF +PT+D
Sbjct: 250 DCLLPWLELHNSCPVCRFELPTDD 264

[Close]
BLAST of Lsi03G006560 vs. TAIR10
Match: AT1G74620.1 (AT1G74620.1 RING/U-box superfamily protein)

HSP 1 Score: 58.2 bits (139), Expect = 8.4e-09
Identity = 30/78 (38.46%), Postives = 42/78 (53.85%), Query Frame = 1 

Query: 162 NSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECI 221
           N A E++L        + ++  E   C ICME+     EG S   V ++PC H FH +CI
Sbjct: 157 NKAAENLLIDSYCYNYLNVATTEENGCAICMEDYI---EGSS--IVAKLPCDHEFHGDCI 216

Query: 222 KKWSKSSHYCPTCRFSMP 240
            KW + +H CP CR S+P
Sbjct: 217 NKWLQLNHMCPLCRSSIP 229

[Close]
BLAST of Lsi03G006560 vs. TAIR10
Match: AT1G18770.1 (AT1G18770.1 RING/U-box superfamily protein)

HSP 1 Score: 57.8 bits (138), Expect = 1.1e-08
Identity = 29/81 (35.80%), Postives = 47/81 (58.02%), Query Frame = 1 

Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA+   ++S+ ++  I ++   S  E   C+IC+EE S       G  V+ +PC H F +
Sbjct: 34  PASKLVVKSLARK--IYKMTTSSTGEM--CIICLEEFSE------GRRVVTLPCGHDFDD 93

Query: 219 ECIKKWSKSSHYCPTCRFSMP 240
           EC+ KW +++H CP CRF +P
Sbjct: 94  ECVLKWFETNHSCPLCRFKLP 104

[Close]
BLAST of Lsi03G006560 vs. TAIR10
Match: AT1G26800.1 (AT1G26800.1 RING/U-box superfamily protein)

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-07
Identity = 31/78 (39.74%), Postives = 39/78 (50.00%), Query Frame = 1 

Query: 164 AMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKK 223
           A ++ +  M I EI    D     CVIC+EE   EE  K      +MPC H FH  CI+K
Sbjct: 93  ASKASIDAMPIVEI----DGCEGECVICLEEWKSEETVK------EMPCKHRFHGGCIEK 152

Query: 224 WSKSSHYCPTCRFSMPTN 242
           W      CP CR+ MP +
Sbjct: 153 WLGFHGSCPVCRYEMPVD 160

[Close]
BLAST of Lsi03G006560 vs. TAIR10
Match: AT1G14200.1 (AT1G14200.1 RING/U-box superfamily protein)

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-07
Identity = 27/84 (32.14%), Postives = 43/84 (51.19%), Query Frame = 1 

Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA  S +E+M + +  E+  K       +C IC++E S+      G    +MPC H FH 
Sbjct: 84  PALKSEVENMPRVVIGEDKEKYGG----SCAICLDEWSK------GDVAAEMPCKHKFHS 143

Query: 219 ECIKKWSKSSHYCPTCRFSMPTND 243
           +C+++W      CP CR+ MP  +
Sbjct: 144 KCVEEWLGRHATCPMCRYEMPVEE 157

[Close]
BLAST of Lsi03G006560 vs. NCBI nr
Match: gi|700188572|gb|KGN43805.1| (hypothetical protein Csa_7G068580 [Cucumis sativus])

HSP 1 Score: 283.1 bits (723), Expect = 4.6e-73
Identity = 145/240 (60.42%), Postives = 181/240 (75.42%), Query Frame = 1 

Query: 8   PIGLRKSARHIQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHP 67
           P+  R+    I+TR+NS TF KQIDLNF+VQLKC  +QI EE   NT++ + E + SQHP
Sbjct: 25  PVQRRRPQTQIRTRRNSPTFFKQIDLNFEVQLKCQHFQIREEETSNTTTLIDEHIVSQHP 84

Query: 68  FATYQILVSILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEED---- 127
           FAT+++ +SIL+HGDKTL+  L ++F  YRDIINI+ + +EIIKYWV KVEEEEED    
Sbjct: 85  FATFRLPISILEHGDKTLKLLLFREFHMYRDIINIEHLVDEIIKYWVTKVEEEEEDQENS 144

Query: 128 -NFRVFGKVYPLEITIELLLFERVD-NDTIEVPLMMVPANNSAMESMLKRMEIEEIMKLS 187
            + RVF K+Y LEIT+ELL+F+ V   D     LMMVP ++SAMESMLKR+E EEIMKL 
Sbjct: 145 TSSRVFKKIYRLEITLELLIFQIVRVMDYQPQVLMMVPTSDSAMESMLKRVENEEIMKLG 204

Query: 188 DDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTN 242
           DD+ I+CVIC+EEI +EE+G  G  VLQMPCLH+FH ECI KW  +SHYCPTCRF MP N
Sbjct: 205 DDDCIHCVICLEEIGKEEKGSEGV-VLQMPCLHVFHGECINKWLNTSHYCPTCRFPMPIN 263

[Close]
BLAST of Lsi03G006560 vs. NCBI nr
Match: gi|700188567|gb|KGN43800.1| (hypothetical protein Csa_7G067550 [Cucumis sativus])

HSP 1 Score: 268.5 bits (685), Expect = 1.2e-68
Identity = 134/231 (58.01%), Postives = 179/231 (77.49%), Query Frame = 1 

Query: 18  IQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSI 77
           IQTR NS T +KQIDLNFKV++KC  +Q  EE     ++FL E + +  PF   Q+ VSI
Sbjct: 25  IQTRSNSPTSVKQIDLNFKVEVKCQHFQAGEEDTTFATTFLSEHIIAHQPFPALQLPVSI 84

Query: 78  LKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLEIT 137
            KHGDKTL+R L Q+F+ +R IINI+LV ++IIK+WV+KVE+++E+N  VF ++YPLEIT
Sbjct: 85  FKHGDKTLKRLLFQKFQMFRAIINIELVTDQIIKHWVKKVEDDKENNSGVFEEIYPLEIT 144

Query: 138 IELLLFERVD-----NDTIEVP-LMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVIC 197
           IE+L+ + +D      +TI+ P + MVP ++SAM+SMLKR+E EEIMK+ D +SI+CVIC
Sbjct: 145 IEVLVLQMIDAVDQPQETIDQPQVRMVPTSDSAMKSMLKRVENEEIMKVGDYKSISCVIC 204

Query: 198 MEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           +E+IS+EE+G  G  VLQMPCLHMFHEECI+KW K+SHYCPTCRFSM  N+
Sbjct: 205 LEKISKEEKGSEGV-VLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMTINN 254

[Close]
BLAST of Lsi03G006560 vs. NCBI nr
Match: gi|659111003|ref|XP_008455525.1| (PREDICTED: uncharacterized protein LOC103495676 [Cucumis melo])

HSP 1 Score: 266.2 bits (679), Expect = 5.8e-68
Identity = 139/228 (60.96%), Postives = 179/228 (78.51%), Query Frame = 1 

Query: 23  NSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSILKHGD 82
           +S T ++QI L FKVQLKC  +Q++E GN ++++ + E + SQH FAT+Q+ +SI KHGD
Sbjct: 40  SSPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHGD 99

Query: 83  KTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEE--EDNFRV-FGKVYPLEITIE 142
           KTL+R L ++F+ Y DIINI+L+ANEIIK+WVRKVEEEE  E+N  V F K+YPLEI I 
Sbjct: 100 KTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIR 159

Query: 143 LLLFERVDN-----DTIEVPLMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICMEE 202
           LL+ + +         I+ PL MVP ++SA+ESMLKR+E EEIMK  DDESINCV+C+EE
Sbjct: 160 LLVSKMIRAVQPRLAMIDQPL-MVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEE 219

Query: 203 ISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           IS+EE+G S T VLQMPCLHMFHEECI+KW K+SHYCPTCRFSMP ++
Sbjct: 220 ISKEEKG-SETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]
BLAST of Lsi03G006560 vs. NCBI nr
Match: gi|659111001|ref|XP_008455524.1| (PREDICTED: RING-H2 finger protein ATL30-like [Cucumis melo])

HSP 1 Score: 262.3 bits (669), Expect = 8.3e-67
Identity = 132/241 (54.77%), Postives = 178/241 (73.86%), Query Frame = 1 

Query: 11  LRKSARHIQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFAT 70
           + ++ + I+TRQNSS +++ IDLNFKVQLKCS + +YE+G   T+SFL E  S  HP AT
Sbjct: 1   MNRTRKRIRTRQNSSKYVQHIDLNFKVQLKCSHFLVYEDGITTTTSFLNEHTSRPHPLAT 60

Query: 71  YQILVSILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEE-----EEDN- 130
           Y++ +SI KHGDK LE++L  +F  YR+ IN  LVA+ +I++WV++VEE+     EE+N 
Sbjct: 61  YKLPISIFKHGDKALEQYLFHKFHVYREFINTTLVADRLIQHWVKRVEEDDHEHQEENNN 120

Query: 131 --FRVFGKVYPLEITIELLLFERVDNDTIEVPLM-MVPANNSAMESMLKRMEIEEIMKLS 190
             F VF K+YPLEIT+E+ + +R   ++   P+M MVP ++SA+ESML+R+E +      
Sbjct: 121 HDFNVFQKIYPLEITLEMRIIQRYVEES---PIMIMVPTSDSAIESMLRRVEND-----W 180

Query: 191 DDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTN 243
           DDE INCVIC+EEIS +EE +S   VLQMPCLHMFH ECI KW K+SHYCPTCRFSMPTN
Sbjct: 181 DDEGINCVICLEEISSKEEEESEKSVLQMPCLHMFHGECINKWLKTSHYCPTCRFSMPTN 233

[Close]
BLAST of Lsi03G006560 vs. NCBI nr
Match: gi|700188568|gb|KGN43801.1| (hypothetical protein Csa_7G067560 [Cucumis sativus])

HSP 1 Score: 260.8 bits (665), Expect = 2.4e-66
Identity = 137/236 (58.05%), Postives = 177/236 (75.00%), Query Frame = 1 

Query: 18  IQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNT--SSFLGEDMSSQHPFATYQILV 77
           I+T  NS T +KQIDLNFKV++KC  Y    EG  +T  ++ L E +SS HP  T Q+ V
Sbjct: 36  IRTPPNSPTLVKQIDLNFKVEVKCQHYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPV 95

Query: 78  SILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLE 137
           SI KHGDKTL+R L ++F+ YR IINI+LV +EIIK+WV+KV+EE   + +VF ++YPLE
Sbjct: 96  SIFKHGDKTLKRLLFREFQIYRAIINIELVVDEIIKHWVKKVDEENSTS-QVFEEIYPLE 155

Query: 138 ITIELLLFERVDNDTIEVP---------LMMVPANNSAMESMLKRMEIEEIMKLSDDESI 197
           ITIELL+++ +    I+ P         +MMVP + SAMESMLK++E EEI+K  D++SI
Sbjct: 156 ITIELLVYQMI--HAIDQPHEIMMDQPQVMMVPTSESAMESMLKKVENEEIVKFGDNKSI 215

Query: 198 NCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           NCVIC+EEIS+E++G S   VLQMPCLHMFHEECI+KW K SHYCPTCRFSMPTN+
Sbjct: 216 NCVICLEEISKEKKG-SEAVVLQMPCLHMFHEECIRKWLKRSHYCPTCRFSMPTNN 267

The following BLAST results are available for this feature:
BLAST of Lsi03G006560 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RNG1L_ARATH2.7e-0934.52E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1... [more]
RING1_GOSHI1.1e-0732.94E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1[more]
YKW3_SCHPO1.6e-0634.62Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (stra... [more]
SIS3_ARATH6.3e-0632.61E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana GN=SIS3 PE=2 SV=2[more]
BLAST of Lsi03G006560 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K2A5_CUCSA3.2e-7360.42Uncharacterized protein OS=Cucumis sativus GN=Csa_7G068580 PE=4 SV=1[more]
A0A0A0K2A1_CUCSA8.1e-6958.01Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067550 PE=4 SV=1[more]
E5GCQ3_CUCME4.0e-6860.96Hypothetical zinc finger protein OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0K7L0_CUCSA1.7e-6658.05Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067560 PE=4 SV=1[more]
A0A0A0K493_CUCSA1.4e-6556.09Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067540 PE=4 SV=1[more]
BLAST of Lsi03G006560 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G19950.11.5e-1034.52 RING/U-box superfamily protein[more]
AT1G74620.18.4e-0938.46 RING/U-box superfamily protein[more]
AT1G18770.11.1e-0835.80 RING/U-box superfamily protein[more]
AT1G26800.11.6e-0739.74 RING/U-box superfamily protein[more]
AT1G14200.11.6e-0732.14 RING/U-box superfamily protein[more]
BLAST of Lsi03G006560 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700188572|gb|KGN43805.1|4.6e-7360.42hypothetical protein Csa_7G068580 [Cucumis sativus][more]
gi|700188567|gb|KGN43800.1|1.2e-6858.01hypothetical protein Csa_7G067550 [Cucumis sativus][more]
gi|659111003|ref|XP_008455525.1|5.8e-6860.96PREDICTED: uncharacterized protein LOC103495676 [Cucumis melo][more]
gi|659111001|ref|XP_008455524.1|8.3e-6754.77PREDICTED: RING-H2 finger protein ATL30-like [Cucumis melo][more]
gi|700188568|gb|KGN43801.1|2.4e-6658.05hypothetical protein Csa_7G067560 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044267 cellular protein metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G006560.1Lsi03G006560.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 187..235
score: 1.1
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 188..234
score: 1.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 188..235
score: 13
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 178..239
score: 9.6
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 92..242
score: 2.9E-20coord: 37..47
score: 2.9
NoneNo IPR availablePANTHERPTHR22763:SF78E3 UBIQUITIN-PROTEIN LIGASE RHA2Acoord: 37..47
score: 2.9E-20coord: 92..242
score: 2.9
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 170..240
score: 2.99

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi03G006560Csa7G067540Cucumber (Chinese Long) v2culsiB518
Lsi03G006560CSPI07G06330Wild cucumber (PI 183967)cpilsiB532
Lsi03G006560Cucsa.218660Cucumber (Gy14) v1cgylsiB364
Lsi03G006560MELO3C018566Melon (DHL92) v3.5.1lsimeB251
Lsi03G006560MELO3C028580.2Melon (DHL92) v3.6.1lsimedB264
Lsi03G006560Bhi05G000815Wax gourdlsiwgoB320
Lsi03G006560CsGy7G005960Cucumber (Gy14) v2cgyblsiB485
Lsi03G006560CsaV3_7G007310Cucumber (Chinese Long) v3cuclsiB552
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi03G006560Melon (DHL92) v3.6.1lsimedB260
Lsi03G006560Melon (DHL92) v3.6.1lsimedB281
Lsi03G006560Silver-seed gourdcarlsiB262
Lsi03G006560Silver-seed gourdcarlsiB671
Lsi03G006560Cucumber (Chinese Long) v3cuclsiB555
Lsi03G006560Watermelon (97103) v2lsiwmbB247
Lsi03G006560Wax gourdlsiwgoB307
Lsi03G006560Bottle gourd (USVL1VR-Ls)lsilsiB126
Lsi03G006560Cucumber (Gy14) v1cgylsiB367
Lsi03G006560Cucurbita maxima (Rimu)cmalsiB116
Lsi03G006560Cucurbita maxima (Rimu)cmalsiB484
Lsi03G006560Cucurbita maxima (Rimu)cmalsiB662
Lsi03G006560Cucurbita moschata (Rifu)cmolsiB103
Lsi03G006560Cucurbita moschata (Rifu)cmolsiB468
Lsi03G006560Cucurbita moschata (Rifu)cmolsiB650
Lsi03G006560Cucurbita pepo (Zucchini)cpelsiB208
Lsi03G006560Cucurbita pepo (Zucchini)cpelsiB543
Lsi03G006560Wild cucumber (PI 183967)cpilsiB536
Lsi03G006560Cucumber (Chinese Long) v2culsiB522
Lsi03G006560Melon (DHL92) v3.5.1lsimeB247
Lsi03G006560Melon (DHL92) v3.5.1lsimeB268
Lsi03G006560Watermelon (Charleston Gray)lsiwcgB212
Lsi03G006560Watermelon (97103) v1lsiwmB227
Lsi03G006560Cucumber (Gy14) v2cgyblsiB490