Bhi05G000815 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi05G000815
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	E3 ubiquitin-protein ligase
Location	chr5 : 34348468 .. 34349232 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCACAACTCTTTGATATTTGTAGGAATGGTTCCTATGTTGGCATGTCACTAGATATTAGAGATCTCATCATAAATCGCCAACCACGAAGACGAATTCGAACTCGACGAAACTCATCAACGTTCGTCAAACGACTCGACCTTAATTTTAAAGTCCAACTCAAATGTAGTCACTACCAAGTATGTGAAGAAGGCAATAGGAGTACAACGACTCTACTCTGTGAACACACAACTTCACAATATCCCCTTGCAAATTACCAACTTCCAATCTCCATCTTCAAGCACCATGACAAAACGTTGGAGCGATTTCTTCTTCGTCAGTTTCAGATGAATCGAGACTTCATCAACATTAAATTTGTGGCTGACAAAATAATACAACATTGGATTAGAAAAGTTGAAGAAGAGAACGATTTTAGTGTATTTCAAAAGATTCATCCTCTCAAAATCACGATAGAGTTGTTGGCTTTTCAGAGAGTTTATGTATTTGAATCATCACCAATGATGGTTCCAACGAGTGATAGTGCGATGAAGAATATGTTAAAGAGAGTGGAAATTGAAGAGATAATGAAATTAGGTGAGGATGAAGGCATTGATTGTGTAATTTGTTTGGAGGAAATTAGTAGAGAGGAGGAAGGAAAAAGTGGGATATTGGTGTTACAAATGCCTTGCTTGCATGTGTTTCATGGAGAATGTATAAGGAAGTGGTTGGAGACTAGTCATTATTGTCCAACTTGTAGATTTGCAATGCCTACAAATAATTGA

mRNA sequence

ATGGCGGCACAACTCTTTGATATTTGTAGGAATGGTTCCTATGTTGGCATGTCACTAGATATTAGAGATCTCATCATAAATCGCCAACCACGAAGACGAATTCGAACTCGACGAAACTCATCAACGTTCGTCAAACGACTCGACCTTAATTTTAAAGTCCAACTCAAATGTAGTCACTACCAAGTATGTGAAGAAGGCAATAGGAGTACAACGACTCTACTCTGTGAACACACAACTTCACAATATCCCCTTGCAAATTACCAACTTCCAATCTCCATCTTCAAGCACCATGACAAAACGTTGGAGCGATTTCTTCTTCGTCAGTTTCAGATGAATCGAGACTTCATCAACATTAAATTTGTGGCTGACAAAATAATACAACATTGGATTAGAAAAGTTGAAGAAGAGAACGATTTTAGTGTATTTCAAAAGATTCATCCTCTCAAAATCACGATAGAGTTGTTGGCTTTTCAGAGAGTTTATGTATTTGAATCATCACCAATGATGGTTCCAACGAGTGATAGTGCGATGAAGAATATGTTAAAGAGAGTGGAAATTGAAGAGATAATGAAATTAGGTGAGGATGAAGGCATTGATTGTGTAATTTGTTTGGAGGAAATTAGTAGAGAGGAGGAAGGAAAAAGTGGGATATTGGTGTTACAAATGCCTTGCTTGCATGTGTTTCATGGAGAATGTATAAGGAAGTGGTTGGAGACTAGTCATTATTGTCCAACTTGTAGATTTGCAATGCCTACAAATAATTGA

Coding sequence (CDS)

ATGGCGGCACAACTCTTTGATATTTGTAGGAATGGTTCCTATGTTGGCATGTCACTAGATATTAGAGATCTCATCATAAATCGCCAACCACGAAGACGAATTCGAACTCGACGAAACTCATCAACGTTCGTCAAACGACTCGACCTTAATTTTAAAGTCCAACTCAAATGTAGTCACTACCAAGTATGTGAAGAAGGCAATAGGAGTACAACGACTCTACTCTGTGAACACACAACTTCACAATATCCCCTTGCAAATTACCAACTTCCAATCTCCATCTTCAAGCACCATGACAAAACGTTGGAGCGATTTCTTCTTCGTCAGTTTCAGATGAATCGAGACTTCATCAACATTAAATTTGTGGCTGACAAAATAATACAACATTGGATTAGAAAAGTTGAAGAAGAGAACGATTTTAGTGTATTTCAAAAGATTCATCCTCTCAAAATCACGATAGAGTTGTTGGCTTTTCAGAGAGTTTATGTATTTGAATCATCACCAATGATGGTTCCAACGAGTGATAGTGCGATGAAGAATATGTTAAAGAGAGTGGAAATTGAAGAGATAATGAAATTAGGTGAGGATGAAGGCATTGATTGTGTAATTTGTTTGGAGGAAATTAGTAGAGAGGAGGAAGGAAAAAGTGGGATATTGGTGTTACAAATGCCTTGCTTGCATGTGTTTCATGGAGAATGTATAAGGAAGTGGTTGGAGACTAGTCATTATTGTCCAACTTGTAGATTTGCAATGCCTACAAATAATTGA

Protein sequence

MAAQLFDICRNGSYVGMSLDIRDLIINRQPRRRIRTRRNSSTFVKRLDLNFKVQLKCSHYQVCEEGNRSTTTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFINIKFVADKIIQHWIRKVEEENDFSVFQKIHPLKITIELLAFQRVYVFESSPMMVPTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHGECIRKWLETSHYCPTCRFAMPTNN

Homology

[Close]

BLAST of Bhi05G000815 vs. Swiss-Prot
Match: sp|Q9SNB6|RDUF1_ARATH (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.9e-09
Identity = 32/83 (38.55%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 171 PTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHG 230
           P S SA+++ L RVEI +     E    +C +C E      EG+      +MPC H+FHG
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAE---ANCAVCTEVFEAGIEGR------EMPCKHIFHG 249

Query: 231 ECIRKWLETSHYCPTCRFAMPTN 254
           +CI  WL   + CP CRF +P++
Sbjct: 250 DCIVPWLSIRNSCPVCRFELPSD 262

[Close]

BLAST of Bhi05G000815 vs. Swiss-Prot
Match: sp|Q8LPN7|RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-08
Identity = 33/84 (39.29%), Postives = 49/84 (58.33%), Query Frame = 0


Query: 171 PTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHG 230
           P S SA+ + L  V++ + M   + E   C +C++E    E+G     V QMPC HVFH 
Sbjct: 190 PASKSAI-DALPTVKVTKDML--KSEMNQCAVCMDEF---EDGSD---VKQMPCKHVFHQ 249

Query: 231 ECIRKWLETSHYCPTCRFAMPTNN 255
           +C+  WLE  + CP CRF +PT++
Sbjct: 250 DCLLPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Bhi05G000815 vs. Swiss-Prot
Match: sp|Q9LQX2|MPSR1_ARATH (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.9e-08
Identity = 31/70 (44.29%), Postives = 40/70 (57.14%), Query Frame = 0


Query: 184 VEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHGECIRKWLETSHYC 243
           ++   I+++   EG +CVICLEE   EE       V +MPC H FHG CI KWL     C
Sbjct: 98  IDAMPIVEIDGCEG-ECVICLEEWKSEE------TVKEMPCKHRFHGGCIEKWLGFHGSC 157

Query: 244 PTCRFAMPTN 254
           P CR+ MP +
Sbjct: 158 PVCRYEMPVD 160

[Close]

BLAST of Bhi05G000815 vs. Swiss-Prot
Match: sp|Q7ZW78|RN181_DANRE (E3 ubiquitin-protein ligase RNF181 OS=Danio rerio OX=7955 GN=rnf181 PE=2 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 1.9e-08
Identity = 28/79 (35.44%), Postives = 43/79 (54.43%), Query Frame = 0


Query: 176 AMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHGECIRK 235
           A K +++ + +  I     D+G+ C +CL E   +E       V +MPC H+FH  CI  
Sbjct: 55  AAKAVVQSLPVVIISPEQADKGVKCPVCLLEFEEQES------VREMPCKHLFHTGCILP 114

Query: 236 WLETSHYCPTCRFAMPTNN 255
           WL  ++ CP CR  +PT+N
Sbjct: 115 WLNKTNSCPLCRLELPTDN 127

[Close]

BLAST of Bhi05G000815 vs. Swiss-Prot
Match: sp|Q940T5|RDUF2_ARATH (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.2e-08
Identity = 31/82 (37.80%), Postives = 46/82 (56.10%), Query Frame = 0


Query: 171 PTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHG 230
           P S SA+++ L RVEI +   +G +   +C +C E    E E +      +MPC H+FH 
Sbjct: 174 PASKSAIES-LPRVEISD-CHIGSE--ANCAVCTEIFETETEAR------EMPCKHLFHD 233

Query: 231 ECIRKWLETSHYCPTCRFAMPT 253
           +CI  WL   + CP CRF +P+
Sbjct: 234 DCIVPWLSIRNSCPVCRFELPS 245

[Close]

BLAST of Bhi05G000815 vs. TAIR10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 63.9 bits (154), Expect = 1.6e-10
Identity = 32/83 (38.55%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 171 PTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHG 230
           P S SA+++ L RVEI +     E    +C +C E      EG+      +MPC H+FHG
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAE---ANCAVCTEVFEAGIEGR------EMPCKHIFHG 249

Query: 231 ECIRKWLETSHYCPTCRFAMPTN 254
           +CI  WL   + CP CRF +P++
Sbjct: 250 DCIVPWLSIRNSCPVCRFELPSD 262

[Close]

BLAST of Bhi05G000815 vs. TAIR10
Match: AT1G18770.1 (RING/U-box superfamily protein)

HSP 1 Score: 63.2 bits (152), Expect = 2.7e-10
Identity = 31/81 (38.27%), Postives = 43/81 (53.09%), Query Frame = 0


Query: 171 PTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHG 230
           P S   +K++ +++        GE     C+ICLEE S       G  V+ +PC H F  
Sbjct: 34  PASKLVVKSLARKIYKMTTSSTGE----MCIICLEEFS------EGRRVVTLPCGHDFDD 93

Query: 231 ECIRKWLETSHYCPTCRFAMP 252
           EC+ KW ET+H CP CRF +P
Sbjct: 94  ECVLKWFETNHSCPLCRFKLP 104

[Close]

BLAST of Bhi05G000815 vs. TAIR10
Match: AT1G74620.1 (RING/U-box superfamily protein)

HSP 1 Score: 61.6 bits (148), Expect = 8.0e-10
Identity = 29/79 (36.71%), Postives = 45/79 (56.96%), Query Frame = 0


Query: 173 SDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHGEC 232
           ++ A +N+L        + +   E   C IC+E+     EG S  +V ++PC H FHG+C
Sbjct: 156 ANKAAENLLIDSYCYNYLNVATTEENGCAICMEDYI---EGSS--IVAKLPCDHEFHGDC 215

Query: 233 IRKWLETSHYCPTCRFAMP 252
           I KWL+ +H CP CR ++P
Sbjct: 216 INKWLQLNHMCPLCRSSIP 229

[Close]

BLAST of Bhi05G000815 vs. TAIR10
Match: AT3G19950.1 (RING/U-box superfamily protein)

HSP 1 Score: 61.6 bits (148), Expect = 8.0e-10
Identity = 33/84 (39.29%), Postives = 49/84 (58.33%), Query Frame = 0


Query: 171 PTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHG 230
           P S SA+ + L  V++ + M   + E   C +C++E    E+G     V QMPC HVFH 
Sbjct: 190 PASKSAI-DALPTVKVTKDML--KSEMNQCAVCMDEF---EDGSD---VKQMPCKHVFHQ 249

Query: 231 ECIRKWLETSHYCPTCRFAMPTNN 255
           +C+  WLE  + CP CRF +PT++
Sbjct: 250 DCLLPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Bhi05G000815 vs. TAIR10
Match: AT5G01980.1 (RING/U-box superfamily protein)

HSP 1 Score: 61.6 bits (148), Expect = 8.0e-10
Identity = 31/85 (36.47%), Postives = 46/85 (54.12%), Query Frame = 0


Query: 171 PTSDSAMKNMLKRVEIEE-IMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFH 230
           P S S ++N+ + +  EE +MK     G+ C IC E  S   E        Q+PCLH++H
Sbjct: 325 PASVSCVRNLPRVIIAEEHVMK-----GLVCAICKELFSLRNE------TTQLPCLHLYH 384

Query: 231 GECIRKWLETSHYCPTCRFAMPTNN 255
             CI  WL   + CP CR+ +PT++
Sbjct: 385 AHCIVPWLSARNSCPLCRYELPTDD 398

[Close]

BLAST of Bhi05G000815 vs. TrEMBL
Match: tr|A0A0A0K2A5|A0A0A0K2A5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G068580 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 8.0e-81
Identity = 159/264 (60.23%), Postives = 197/264 (74.62%), Query Frame = 0


Query: 1   MAAQLFDICRNGSYVGMSLDI-RDLIINRQPRRRIRTRRNSSTFVKRLDLNFKVQLKCSH 60
           MA ++FD CR GSY+GM L I  D +  R+P+ +IRTRRNS TF K++DLNF+VQLKC H
Sbjct: 1   MAERIFDRCRCGSYMGMKLRICDDPVQRRRPQTQIRTRRNSPTFFKQIDLNFEVQLKCQH 60

Query: 61  YQVCEEGNRSTTTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFINIK 120
           +Q+ EE   +TTTL+ EH  SQ+P A ++LPISI +H DKTL+  L R+F M RD INI+
Sbjct: 61  FQIREEETSNTTTLIDEHIVSQHPFATFRLPISILEHGDKTLKLLLFREFHMYRDIINIE 120

Query: 121 FVADKIIQHWIRKVEE-------ENDFSVFQKIHPLKITIELLAFQRVYVFESSP---MM 180
            + D+II++W+ KVEE            VF+KI+ L+IT+ELL FQ V V +  P   MM
Sbjct: 121 HLVDEIIKYWVTKVEEXXXXXXXXXSSRVFKKIYRLEITLELLIFQIVRVMDYQPQVLMM 180

Query: 181 VPTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFH 240
           VPTSDSAM++MLKRVE EEIMKLG+D+ I CVICLEEI +EE+G  G+ VLQMPCLHVFH
Sbjct: 181 VPTSDSAMESMLKRVENEEIMKLGDDDCIHCVICLEEIGKEEKGSEGV-VLQMPCLHVFH 240

Query: 241 GECIRKWLETSHYCPTCRFAMPTN 254
           GECI KWL TSHYCPTCRF MP N
Sbjct: 241 GECINKWLNTSHYCPTCRFPMPIN 263

[Close]

BLAST of Bhi05G000815 vs. TrEMBL
Match: tr|A0A0A0K7L0|A0A0A0K7L0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G067560 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.4e-79
Identity = 154/268 (57.46%), Postives = 200/268 (74.63%), Query Frame = 0


Query: 1   MAAQLFDICR--NGSYVGMSLDIRDLIINRQPRRRIRTRRNSSTFVKRLDLNFKVQLKCS 60
           MA Q+F  CR  +GSY+GMSL I D  +  QP+ +IRT  NS T VK++DLNFKV++KC 
Sbjct: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60

Query: 61  HYQVCEEGNRST--TTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFI 120
           HY    EG  ST  T LL EH +S +PL   QLP+SIFKH DKTL+R L R+FQ+ R  I
Sbjct: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120

Query: 121 NIKFVADKIIQHWIRKVEEENDFS-VFQKIHPLKITIELLAFQRVYVFES---------S 180
           NI+ V D+II+HW++KV+EEN  S VF++I+PL+ITIELL +Q ++  +           
Sbjct: 121 NIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQ 180

Query: 181 PMMVPTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLH 240
            MMVPTS+SAM++MLK+VE EEI+K G+++ I+CVICLEEIS+E++G   + VLQMPCLH
Sbjct: 181 VMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAV-VLQMPCLH 240

Query: 241 VFHGECIRKWLETSHYCPTCRFAMPTNN 255
           +FH ECIRKWL+ SHYCPTCRF+MPTNN
Sbjct: 241 MFHEECIRKWLKRSHYCPTCRFSMPTNN 267

[Close]

BLAST of Bhi05G000815 vs. TrEMBL
Match: tr|A0A0A0K7L5|A0A0A0K7L5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G068590 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.6e-76
Identity = 159/270 (58.89%), Postives = 205/270 (75.93%), Query Frame = 0


Query: 1   MAAQLFDICRNGSYVGMSLDIRDLIIN-RQPRRRIRTRRNSSTFVKRLDLNFKVQLKCSH 60
           MAAQ+FD+C N  +VGMSL I+D I +  + R+RIRTRRNSS +V+ +DLNFKVQL+C+H
Sbjct: 1   MAAQIFDMCMNRPFVGMSLKIKDSITSLHRTRKRIRTRRNSSKYVEHIDLNFKVQLRCTH 60

Query: 61  -YQVCEEGNRSTTTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFINI 120
             Q  E+G  +TT+ L EHT+S +PLA YQLPISIFK+ + TLE++L  +   +R+ I+I
Sbjct: 61  VLQEYEDGIMTTTSFLHEHTSSSHPLATYQLPISIFKNGNSTLEQYLCDRLHAHRELIDI 120

Query: 121 KFVADKIIQHWIRKVEEEN----------DFSVFQKIHPLKITIELLAFQRVYVFESSPM 180
             VAD+IIQHW++KVEE++          DF+VFQKIH L+IT+E +   R YV ES  M
Sbjct: 121 TLVADRIIQHWVKKVEEDDCDHDEEENNEDFNVFQKIHSLEITLE-MKINRRYVVESPVM 180

Query: 181 -MVPTSDSAMKNMLKRVEIEEIMKLG-EDEGIDCVICLEEIS--REEEGKSGILVLQMPC 240
            MVPTSD A+++ML+RVE +EI +LG ++EGI+C ICLEEIS  +EEEG S   VLQMPC
Sbjct: 181 IMVPTSDDAIESMLRRVENDEIRRLGCDEEGIECAICLEEISSDKEEEG-SEKSVLQMPC 240

Query: 241 LHVFHGECIRKWLETSHYCPTCRFAMPTNN 255
           LH+FH ECI KWL TSHYCPTCRF+MPTNN
Sbjct: 241 LHMFHEECIDKWLRTSHYCPTCRFSMPTNN 268

[Close]

BLAST of Bhi05G000815 vs. TrEMBL
Match: tr|E5GCQ3|E5GCQ3_CUCME (Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.0e-75
Identity = 152/267 (56.93%), Postives = 197/267 (73.78%), Query Frame = 0


Query: 1   MAAQLFDICR--NGSYVGMSLDIRDLIINRQPRRRIRTRRNSSTFVKRLDLNFKVQLKCS 60
           MA ++F+ICR  +GSY+GMSL I D     QP  +I  + +S T V+++ L FKVQLKC 
Sbjct: 1   MAEEIFNICRYGSGSYMGMSLRITDPNPRHQPLNQI-DQTSSPTLVEQICLKFKVQLKCQ 60

Query: 61  HYQVCEEGNRSTTTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFINI 120
           H+QV E GN  +TTL+ EH  SQ+  A +QLPISIFKH DKTL+R L R+F+M  D INI
Sbjct: 61  HFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHGDKTLKRILFREFKMYGDIINI 120

Query: 121 KFVADKIIQHWIRKVEEENDFS-----VFQKIHPLKITIELLAFQRVYVFE------SSP 180
           + +A++II+HW+RKVEEE D       +F+KI+PL+I I LL  + +   +        P
Sbjct: 121 ELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIRLLVSKMIRAVQPRLAMIDQP 180

Query: 181 MMVPTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHV 240
           +MVPTSDSA+++MLKRVE EEIMK G+DE I+CV+CLEEIS+EE+G S   VLQMPCLH+
Sbjct: 181 LMVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEEISKEEKG-SETTVLQMPCLHM 240

Query: 241 FHGECIRKWLETSHYCPTCRFAMPTNN 255
           FH ECIRKWL+TSHYCPTCRF+MP +N
Sbjct: 241 FHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of Bhi05G000815 vs. TrEMBL
Match: tr|A0A0A0K2A1|A0A0A0K2A1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G067550 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.2e-73
Identity = 148/264 (56.06%), Postives = 187/264 (70.83%), Query Frame = 0


Query: 1   MAAQLFDICRNGSYVGMSLDIRDLIINRQPRRRIRTRRNSSTFVKRLDLNFKVQLKCSHY 60
           MA Q FD C +G Y+GMSL I D          I+TR NS T VK++DLNFKV++KC H+
Sbjct: 1   MAEQNFDDCTSGYYMGMSLRITD---------PIQTRSNSPTSVKQIDLNFKVEVKCQHF 60

Query: 61  QVCEEGNRSTTTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFINIKF 120
           Q  EE     TT L EH  +  P    QLP+SIFKH DKTL+R L ++FQM R  INI+ 
Sbjct: 61  QAGEEDTTFATTFLSEHIIAHQPFPALQLPVSIFKHGDKTLKRLLFQKFQMFRAIINIEL 120

Query: 121 VADKIIQHWIRKVEE--ENDFSVFQKIHPLKITIELLAFQRV--------YVFESSPMMV 180
           V D+II+HW++KVE+  EN+  VF++I+PL+ITIE+L  Q +         + +    MV
Sbjct: 121 VTDQIIKHWVKKVEDDKENNSGVFEEIYPLEITIEVLVLQMIDAVDQPQETIDQPQVRMV 180

Query: 181 PTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHG 240
           PTSDSAMK+MLKRVE EEIMK+G+ + I CVICLE+IS+EE+G  G+ VLQMPCLH+FH 
Sbjct: 181 PTSDSAMKSMLKRVENEEIMKVGDYKSISCVICLEKISKEEKGSEGV-VLQMPCLHMFHE 240

Query: 241 ECIRKWLETSHYCPTCRFAMPTNN 255
           ECIRKWL+TSHYCPTCRF+M  NN
Sbjct: 241 ECIRKWLKTSHYCPTCRFSMTINN 254

[Close]

BLAST of Bhi05G000815 vs. NCBI nr
Match: KGN43805.1 (hypothetical protein Csa_7G068580 [Cucumis sativus])

HSP 1 Score: 309.3 bits (791), Expect = 1.2e-80
Identity = 159/264 (60.23%), Postives = 197/264 (74.62%), Query Frame = 0


Query: 1   MAAQLFDICRNGSYVGMSLDI-RDLIINRQPRRRIRTRRNSSTFVKRLDLNFKVQLKCSH 60
           MA ++FD CR GSY+GM L I  D +  R+P+ +IRTRRNS TF K++DLNF+VQLKC H
Sbjct: 1   MAERIFDRCRCGSYMGMKLRICDDPVQRRRPQTQIRTRRNSPTFFKQIDLNFEVQLKCQH 60

Query: 61  YQVCEEGNRSTTTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFINIK 120
           +Q+ EE   +TTTL+ EH  SQ+P A ++LPISI +H DKTL+  L R+F M RD INI+
Sbjct: 61  FQIREEETSNTTTLIDEHIVSQHPFATFRLPISILEHGDKTLKLLLFREFHMYRDIINIE 120

Query: 121 FVADKIIQHWIRKVEE-------ENDFSVFQKIHPLKITIELLAFQRVYVFESSP---MM 180
            + D+II++W+ KVEE            VF+KI+ L+IT+ELL FQ V V +  P   MM
Sbjct: 121 HLVDEIIKYWVTKVEEXXXXXXXXXSSRVFKKIYRLEITLELLIFQIVRVMDYQPQVLMM 180

Query: 181 VPTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFH 240
           VPTSDSAM++MLKRVE EEIMKLG+D+ I CVICLEEI +EE+G  G+ VLQMPCLHVFH
Sbjct: 181 VPTSDSAMESMLKRVENEEIMKLGDDDCIHCVICLEEIGKEEKGSEGV-VLQMPCLHVFH 240

Query: 241 GECIRKWLETSHYCPTCRFAMPTN 254
           GECI KWL TSHYCPTCRF MP N
Sbjct: 241 GECINKWLNTSHYCPTCRFPMPIN 263

[Close]

BLAST of Bhi05G000815 vs. NCBI nr
Match: KGN43801.1 (hypothetical protein Csa_7G067560 [Cucumis sativus])

HSP 1 Score: 303.9 bits (777), Expect = 5.1e-79
Identity = 154/268 (57.46%), Postives = 200/268 (74.63%), Query Frame = 0


Query: 1   MAAQLFDICR--NGSYVGMSLDIRDLIINRQPRRRIRTRRNSSTFVKRLDLNFKVQLKCS 60
           MA Q+F  CR  +GSY+GMSL I D  +  QP+ +IRT  NS T VK++DLNFKV++KC 
Sbjct: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60

Query: 61  HYQVCEEGNRST--TTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFI 120
           HY    EG  ST  T LL EH +S +PL   QLP+SIFKH DKTL+R L R+FQ+ R  I
Sbjct: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120

Query: 121 NIKFVADKIIQHWIRKVEEENDFS-VFQKIHPLKITIELLAFQRVYVFES---------S 180
           NI+ V D+II+HW++KV+EEN  S VF++I+PL+ITIELL +Q ++  +           
Sbjct: 121 NIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQ 180

Query: 181 PMMVPTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLH 240
            MMVPTS+SAM++MLK+VE EEI+K G+++ I+CVICLEEIS+E++G   + VLQMPCLH
Sbjct: 181 VMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAV-VLQMPCLH 240

Query: 241 VFHGECIRKWLETSHYCPTCRFAMPTNN 255
           +FH ECIRKWL+ SHYCPTCRF+MPTNN
Sbjct: 241 MFHEECIRKWLKRSHYCPTCRFSMPTNN 267

[Close]

BLAST of Bhi05G000815 vs. NCBI nr
Match: XP_011659722.1 (PREDICTED: uncharacterized protein LOC105436242 [Cucumis sativus] >KGN43806.1 hypothetical protein Csa_7G068590 [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 2.4e-76
Identity = 159/270 (58.89%), Postives = 205/270 (75.93%), Query Frame = 0


Query: 1   MAAQLFDICRNGSYVGMSLDIRDLIIN-RQPRRRIRTRRNSSTFVKRLDLNFKVQLKCSH 60
           MAAQ+FD+C N  +VGMSL I+D I +  + R+RIRTRRNSS +V+ +DLNFKVQL+C+H
Sbjct: 1   MAAQIFDMCMNRPFVGMSLKIKDSITSLHRTRKRIRTRRNSSKYVEHIDLNFKVQLRCTH 60

Query: 61  -YQVCEEGNRSTTTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFINI 120
             Q  E+G  +TT+ L EHT+S +PLA YQLPISIFK+ + TLE++L  +   +R+ I+I
Sbjct: 61  VLQEYEDGIMTTTSFLHEHTSSSHPLATYQLPISIFKNGNSTLEQYLCDRLHAHRELIDI 120

Query: 121 KFVADKIIQHWIRKVEEEN----------DFSVFQKIHPLKITIELLAFQRVYVFESSPM 180
             VAD+IIQHW++KVEE++          DF+VFQKIH L+IT+E +   R YV ES  M
Sbjct: 121 TLVADRIIQHWVKKVEEDDCDHDEEENNEDFNVFQKIHSLEITLE-MKINRRYVVESPVM 180

Query: 181 -MVPTSDSAMKNMLKRVEIEEIMKLG-EDEGIDCVICLEEIS--REEEGKSGILVLQMPC 240
            MVPTSD A+++ML+RVE +EI +LG ++EGI+C ICLEEIS  +EEEG S   VLQMPC
Sbjct: 181 IMVPTSDDAIESMLRRVENDEIRRLGCDEEGIECAICLEEISSDKEEEG-SEKSVLQMPC 240

Query: 241 LHVFHGECIRKWLETSHYCPTCRFAMPTNN 255
           LH+FH ECI KWL TSHYCPTCRF+MPTNN
Sbjct: 241 LHMFHEECIDKWLRTSHYCPTCRFSMPTNN 268

[Close]

BLAST of Bhi05G000815 vs. NCBI nr
Match: ADN34252.1 (hypothetical zinc finger protein [Cucumis melo subsp. melo])

HSP 1 Score: 290.0 bits (741), Expect = 7.6e-75
Identity = 152/267 (56.93%), Postives = 197/267 (73.78%), Query Frame = 0


Query: 1   MAAQLFDICR--NGSYVGMSLDIRDLIINRQPRRRIRTRRNSSTFVKRLDLNFKVQLKCS 60
           MA ++F+ICR  +GSY+GMSL I D     QP  +I  + +S T V+++ L FKVQLKC 
Sbjct: 1   MAEEIFNICRYGSGSYMGMSLRITDPNPRHQPLNQI-DQTSSPTLVEQICLKFKVQLKCQ 60

Query: 61  HYQVCEEGNRSTTTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFINI 120
           H+QV E GN  +TTL+ EH  SQ+  A +QLPISIFKH DKTL+R L R+F+M  D INI
Sbjct: 61  HFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHGDKTLKRILFREFKMYGDIINI 120

Query: 121 KFVADKIIQHWIRKVEEENDFS-----VFQKIHPLKITIELLAFQRVYVFE------SSP 180
           + +A++II+HW+RKVEEE D       +F+KI+PL+I I LL  + +   +        P
Sbjct: 121 ELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIRLLVSKMIRAVQPRLAMIDQP 180

Query: 181 MMVPTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHV 240
           +MVPTSDSA+++MLKRVE EEIMK G+DE I+CV+CLEEIS+EE+G S   VLQMPCLH+
Sbjct: 181 LMVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEEISKEEKG-SETTVLQMPCLHM 240

Query: 241 FHGECIRKWLETSHYCPTCRFAMPTNN 255
           FH ECIRKWL+TSHYCPTCRF+MP +N
Sbjct: 241 FHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of Bhi05G000815 vs. NCBI nr
Match: KGN43800.1 (hypothetical protein Csa_7G067550 [Cucumis sativus])

HSP 1 Score: 285.4 bits (729), Expect = 1.9e-73
Identity = 148/264 (56.06%), Postives = 187/264 (70.83%), Query Frame = 0


Query: 1   MAAQLFDICRNGSYVGMSLDIRDLIINRQPRRRIRTRRNSSTFVKRLDLNFKVQLKCSHY 60
           MA Q FD C +G Y+GMSL I D          I+TR NS T VK++DLNFKV++KC H+
Sbjct: 1   MAEQNFDDCTSGYYMGMSLRITD---------PIQTRSNSPTSVKQIDLNFKVEVKCQHF 60

Query: 61  QVCEEGNRSTTTLLCEHTTSQYPLANYQLPISIFKHHDKTLERFLLRQFQMNRDFINIKF 120
           Q  EE     TT L EH  +  P    QLP+SIFKH DKTL+R L ++FQM R  INI+ 
Sbjct: 61  QAGEEDTTFATTFLSEHIIAHQPFPALQLPVSIFKHGDKTLKRLLFQKFQMFRAIINIEL 120

Query: 121 VADKIIQHWIRKVEE--ENDFSVFQKIHPLKITIELLAFQRV--------YVFESSPMMV 180
           V D+II+HW++KVE+  EN+  VF++I+PL+ITIE+L  Q +         + +    MV
Sbjct: 121 VTDQIIKHWVKKVEDDKENNSGVFEEIYPLEITIEVLVLQMIDAVDQPQETIDQPQVRMV 180

Query: 181 PTSDSAMKNMLKRVEIEEIMKLGEDEGIDCVICLEEISREEEGKSGILVLQMPCLHVFHG 240
           PTSDSAMK+MLKRVE EEIMK+G+ + I CVICLE+IS+EE+G  G+ VLQMPCLH+FH 
Sbjct: 181 PTSDSAMKSMLKRVENEEIMKVGDYKSISCVICLEKISKEEKGSEGV-VLQMPCLHMFHE 240

Query: 241 ECIRKWLETSHYCPTCRFAMPTNN 255
           ECIRKWL+TSHYCPTCRF+M  NN
Sbjct: 241 ECIRKWLKTSHYCPTCRFSMTINN 254

The following BLAST results are available for this feature:

BLAST of Bhi05G000815 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9SNB6\|RDUF1_ARATH	2.9e-09	38.55	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
sp\|Q8LPN7\|RNG1L_ARATH	1.4e-08	39.29	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
sp\|Q9LQX2\|MPSR1_ARATH	1.9e-08	44.29	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
sp\|Q7ZW78\|RN181_DANRE	1.9e-08	35.44	E3 ubiquitin-protein ligase RNF181 OS=Danio rerio OX=7955 GN=rnf181 PE=2 SV=2	[more]
sp\|Q940T5\|RDUF2_ARATH	4.2e-08	37.80	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]

BLAST of Bhi05G000815 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G46620.1	1.6e-10	38.55	zinc finger (C3HC4-type RING finger) family protein	[more]
AT1G18770.1	2.7e-10	38.27	RING/U-box superfamily protein	[more]
AT1G74620.1	8.0e-10	36.71	RING/U-box superfamily protein	[more]
AT3G19950.1	8.0e-10	39.29	RING/U-box superfamily protein	[more]
AT5G01980.1	8.0e-10	36.47	RING/U-box superfamily protein	[more]

BLAST of Bhi05G000815 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0K2A5\|A0A0A0K2A5_CUCSA	8.0e-81	60.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G068580 PE=4 SV=1	[more]
tr\|A0A0A0K7L0\|A0A0A0K7L0_CUCSA	3.4e-79	57.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G067560 PE=4 SV=1	[more]
tr\|A0A0A0K7L5\|A0A0A0K7L5_CUCSA	1.6e-76	58.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G068590 PE=4 SV=1	[more]
tr\|E5GCQ3\|E5GCQ3_CUCME	5.0e-75	56.93	Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
tr\|A0A0A0K2A1\|A0A0A0K2A1_CUCSA	1.2e-73	56.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G067550 PE=4 SV=1	[more]

BLAST of Bhi05G000815 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN43805.1	1.2e-80	60.23	hypothetical protein Csa_7G068580 [Cucumis sativus]	[more]
KGN43801.1	5.1e-79	57.46	hypothetical protein Csa_7G067560 [Cucumis sativus]	[more]
XP_011659722.1	2.4e-76	58.89	PREDICTED: uncharacterized protein LOC105436242 [Cucumis sativus] >KGN43806.1 hy...	[more]
ADN34252.1	7.6e-75	56.93	hypothetical zinc finger protein [Cucumis melo subsp. melo]	[more]
KGN43800.1	1.9e-73	56.06	hypothetical protein Csa_7G067550 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR013083	Znf_RING/FYVE/PHD
IPR011016	Znf_RING-CH
IPR001841	Znf_RING

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030968	endoplasmic reticulum unfolded protein response
biological_process	GO:0030433	ER-associated ubiquitin-dependent protein catabolic process
biological_process	GO:0070936	protein K48-linked ubiquitination
biological_process	GO:0030970	retrograde protein transport, ER to cytosol
biological_process	GO:0008150	biological_process
biological_process	GO:0044267	cellular protein metabolic process
cellular_component	GO:0036513	Derlin-1 retrotranslocation complex
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:1990381	ubiquitin-specific protease binding
molecular_function	GO:0051082	unfolded protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0005515	protein binding
molecular_function	GO:0016874	ligase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000815	Bhi05M000815	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 200..246 e-value: 1.7E-7 score: 40.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 199..247 e-value: 5.8E-13 score: 48.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 200..247 score: 12.784
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 199..247 e-value: 0.0086 score: 7.7
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 162..253 e-value: 8.2E-18 score: 65.8
None	No IPR available	PANTHER	PTHR45491	FAMILY NOT NAMED	coord: 180..253
None	No IPR available	SUPERFAMILY	SSF57850	RING/U-box	coord: 183..251

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi05G000815	Csa7G067540	Cucumber (Chinese Long) v2	cuwgoB674
Bhi05G000815	MELO3C018566	Melon (DHL92) v3.5.1	mewgoB218
Bhi05G000815	CSPI07G06330	Wild cucumber (PI 183967)	cpiwgoB685
Bhi05G000815	Cucsa.218660	Cucumber (Gy14) v1	cgywgoB450
Bhi05G000815	Lsi03G006560	Bottle gourd (USVL1VR-Ls)	lsiwgoB320
Bhi05G000815	CsGy7G005960	Cucumber (Gy14) v2	cgybwgoB645
Bhi05G000815	MELO3C028580.2	Melon (DHL92) v3.6.1	medwgoB218
Bhi05G000815	CsaV3_7G007310	Cucumber (Chinese Long) v3	cucwgoB699

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi05G000815	Wax gourd	wgowgoB002
Bhi05G000815	Cucumber (Gy14) v1	cgywgoB445
Bhi05G000815	Cucumber (Gy14) v2	cgybwgoB632
Bhi05G000815	Cucurbita maxima (Rimu)	cmawgoB0116
Bhi05G000815	Cucurbita maxima (Rimu)	cmawgoB0642
Bhi05G000815	Cucurbita moschata (Rifu)	cmowgoB0120
Bhi05G000815	Cucurbita moschata (Rifu)	cmowgoB0642
Bhi05G000815	Cucurbita pepo (Zucchini)	cpewgoB0317
Bhi05G000815	Cucurbita pepo (Zucchini)	cpewgoB0856
Bhi05G000815	Wild cucumber (PI 183967)	cpiwgoB387
Bhi05G000815	Wild cucumber (PI 183967)	cpiwgoB672
Bhi05G000815	Cucumber (Chinese Long) v3	cucwgoB686
Bhi05G000815	Cucumber (Chinese Long) v2	cuwgoB661
Bhi05G000815	Melon (DHL92) v3.6.1	medwgoB592
Bhi05G000815	Melon (DHL92) v3.5.1	mewgoB586
Bhi05G000815	Watermelon (Charleston Gray)	wcgwgoB046
Bhi05G000815	Watermelon (97103) v2	wgowmbB275
Bhi05G000815	Watermelon (97103) v1	wgowmB253
Bhi05G000815	Silver-seed gourd	carwgoB0377
Bhi05G000815	Silver-seed gourd	carwgoB0867