Lsi03G003970 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G003970
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	50S ribosomal protein L27
Location	chr03 : 4561547 .. 4562923 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGCGTTTCATTTTAGCCTCGAGTCTGGCTTTACGAGTTTGTGTTGTGCAGTGCAGGCTTTGTGGCGATCCTGCTTCTAACTTCTAAGGATAAGGTTCGCATTCACTCCCCTCTGTCTCTTTCCCTCCTCCATATCCATTTCCTTTCTCTATTAACTCATTCTCTGAATCTGAAACCTCAATCAATTTCTTGTCTTCCCCCAAGAATTCCCCGTTGCAGCCATGAGTTTAAACCTAGTTGCTGCATTCACTGGTCTCTCTCTCGCCTCTTCTTCCTCTTTCTTCAAAGGCGATTTCGGCTCTATTCAGCCCTCTCCCAAGCTCTCTGTCTCCTTCCCCAACAGATTCCCTTTAACCATTGAAAACGCCCACAAGAAAGGAGCTGGAAGCACCAAGAACGGCCGTGATTCCAAGGGCCAGAGACTCGGCGTTAAAATCTTCGGCGACCAAGTTGCTAAGCCTGGTTCCATCATCGTTCGTCAGCGTGGAACTAAGGTAATTTTGAATTTTACATGGAGTTGATTCCTATGGTACCCGTTTCTAGGGCATTCTCCAGCTACATACATCCAACTTCTTTGTAATTGAGTCCTATGGTTTCTGTTTCTGTACCAATCATTTCAGTTTCATGCCGGAAAGAATGTGGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGATGGGCTTGTAAAGTTCGAGAAGTATGGACCTGACAAGAAGAAGGTTTCACTTCGAATCTGAAAGGTTATATTTCAAGAATGAAACACAAAACTGAGATATATTTATTTACTTCATCGTTCATTGCATTGCAGGTGAGTGTTTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTATAGAGCAAGGAAGAGGGAGTATTTCAGGCTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCATTATTGTTCAACCCCAATTAGTATTAGCTTCCACTGATGCTACTGAAACCAATCCAGTTTGCTGATTTATAAAGCCAGAATTTCAATCACTGCTTCTTTAAAATCCCTGATGATCTTGCAGATAGAAGGTCTGCTTCATTAGCCTCTCTTTTTTGGTTGTGCACATTTATTAATTTCCCAGATTCTTCACAGGAATGTGTTTCATTATTATGATTCTCAATTAGCAATACTCGTTTTGTTCTACGTTTTGCCTATACATTTTCTCTTTAGTCCATTCCACTACTTACTACTTTTTGTTGATTTTGATGTCAAAAATGGTATTCAACAGAACAGATAATAGGGGTGAGTTTTGATTTATTTTGTGTTGAAATTACAAACCAAGAAATTAGATGGATTTGGTGGTTCTTCCCTGTACCAACATTACTAGACTGTGATATGAATCATGTAGCTTTGG

mRNA sequence

GGGCGTTTCATTTTAGCCTCGAGTCTGGCTTTACGAGTTTGTGTTGTGCAGTGCAGGCTTTGTGGCGATCCTGCTTCTAACTTCTAAGGATAAGAATTCCCCGTTGCAGCCATGAGTTTAAACCTAGTTGCTGCATTCACTGGTCTCTCTCTCGCCTCTTCTTCCTCTTTCTTCAAAGGCGATTTCGGCTCTATTCAGCCCTCTCCCAAGCTCTCTGTCTCCTTCCCCAACAGATTCCCTTTAACCATTGAAAACGCCCACAAGAAAGGAGCTGGAAGCACCAAGAACGGCCGTGATTCCAAGGGCCAGAGACTCGGCGTTAAAATCTTCGGCGACCAAGTTGCTAAGCCTGGTTCCATCATCGTTCGTCAGCGTGGAACTAAGTTTCATGCCGGAAAGAATGTGGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGATGGGCTTGTAAAGTTCGAGAAGTATGGACCTGACAAGAAGAAGGTGAGTGTTTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTATAGAGCAAGGAAGAGGGAGTATTTCAGGCTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCATTATTGTTCAACCCCAATTAGTATTAGCTTCCACTGATGCTACTGAAACCAATCCAGTTTGCTGATTTATAAAGCCAGAATTTCAATCACTGCTTCTTTAAAATCCCTGATGATCTTGCAGATAGAAGGTCTGCTTCATTAGCCTCTCTTTTTTGGTTGTGCACATTTATTAATTTCCCAGATTCTTCACAGGAATGTGTTTCATTATTATGATTCTCAATTAGCAATACTCGTTTTGTTCTACGTTTTGCCTATACATTTTCTCTTTAGTCCATTCCACTACTTACTACTTTTTGTTGATTTTGATGTCAAAAATGGTATTCAACAGAACAGATAATAGGGGTGAGTTTTGATTTATTTTGTGTTGAAATTACAAACCAAGAAATTAGATGGATTTGGTGGTTCTTCCCTGTACCAACATTACTAGACTGTGATATGAATCATGTAGCTTTGG

Coding sequence (CDS)

ATGAGTTTAAACCTAGTTGCTGCATTCACTGGTCTCTCTCTCGCCTCTTCTTCCTCTTTCTTCAAAGGCGATTTCGGCTCTATTCAGCCCTCTCCCAAGCTCTCTGTCTCCTTCCCCAACAGATTCCCTTTAACCATTGAAAACGCCCACAAGAAAGGAGCTGGAAGCACCAAGAACGGCCGTGATTCCAAGGGCCAGAGACTCGGCGTTAAAATCTTCGGCGACCAAGTTGCTAAGCCTGGTTCCATCATCGTTCGTCAGCGTGGAACTAAGTTTCATGCCGGAAAGAATGTGGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGATGGGCTTGTAAAGTTCGAGAAGTATGGACCTGACAAGAAGAAGGTGAGTGTTTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTATAGAGCAAGGAAGAGGGAGTATTTCAGGCTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCATTATTGTTCAACCCCAATTAGTATTAGCTTCCACTGATGCTACTGAAACCAATCCAGTTTGCTGA

Protein sequence

MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATETNPVC

Homology

[Close]

BLAST of Lsi03G003970 vs. Swiss-Prot
Match: RK27_TOBAC (50S ribosomal protein L27, chloroplastic OS=Nicotiana tabacum GN=RPL27 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 1.7e-72
Identity = 140/167 (83.83%), Postives = 153/167 (91.62%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQP-SPKLSVSFPNRFPLTIENAHKKGAGSTKN 60
           +S +LV AF GLSLASSSSF KGDFG+  P +PK SVSFP + PLTIE+AHKKGAGSTKN
Sbjct: 3   VSFSLVGAFKGLSLASSSSFLKGDFGAAFPVAPKFSVSFPLKSPLTIESAHKKGAGSTKN 62

Query: 61  GRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKY 120
           GRDS GQRLGVKIFGDQVAKPGSIIVRQRGTKFH GKNVGLGKDHTIFSLIDGLVKFEK+
Sbjct: 63  GRDSPGQRLGVKIFGDQVAKPGSIIVRQRGTKFHPGKNVGLGKDHTIFSLIDGLVKFEKF 122

Query: 121 GPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQ 167
           GPD+KK+SVYPREVQPENPNSYR RKRE FRLQRER+KAR+EG+I+Q
Sbjct: 123 GPDRKKISVYPREVQPENPNSYRNRKRESFRLQRERRKARREGVILQ 169

[Close]

BLAST of Lsi03G003970 vs. Swiss-Prot
Match: RK27_ARATH (50S ribosomal protein L27, chloroplastic OS=Arabidopsis thaliana GN=RPL27 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 5.7e-68
Identity = 138/193 (71.50%), Postives = 156/193 (80.83%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNR-----FPLTIENAHKKGAG 60
           MSLNL+ AF GLSL+S+SSF +GD  S  P    +V+ P        PLTIE+AHKKGAG
Sbjct: 7   MSLNLIGAFKGLSLSSTSSFLRGDL-SFSPKTSFTVTLPLENLQAPIPLTIESAHKKGAG 66

Query: 61  STKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVK 120
           STKNGRDS GQRLGVKI+GDQVAKPG+IIVRQRGTKFHAGKNVG+GKDHTIFSLIDGLVK
Sbjct: 67  STKNGRDSPGQRLGVKIYGDQVAKPGAIIVRQRGTKFHAGKNVGIGKDHTIFSLIDGLVK 126

Query: 121 FEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG---IIVQPQLVL- 180
           FEK+GPD+KK+SVYPRE+ PENPNSYRARKRE FRLQRE+KKAR+E     +  P+LVL 
Sbjct: 127 FEKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQREKKKARRENYSYTLPTPELVLA 186

Query: 181 -ASTDATETNPVC 184
            AS D  E NP C
Sbjct: 187 SASVDDAEANPEC 198

[Close]

BLAST of Lsi03G003970 vs. Swiss-Prot
Match: RK27_ORYSJ (50S ribosomal protein L27, chloroplastic OS=Oryza sativa subsp. japonica GN=RPL27 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.0e-60
Identity = 129/193 (66.84%), Postives = 149/193 (77.20%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSLAS------SSSFFKGDFGSIQPSPKLSVSFPNRFP---LTIENAHK 60
           M+  LV AF G+SL+S      S+SF + D  S+     + +  P   P   LTI+ AHK
Sbjct: 4   MAFTLVGAFKGMSLSSPCHSSSSASFLRADRVSLSVGGGVGMGVPMTMPVRRLTIQMAHK 63

Query: 61  KGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLID 120
           KGAGSTKNGRDS GQRLGVKI+GDQVAKPG+II+RQRGT+ + G NVG+GKDHT+FSLID
Sbjct: 64  KGAGSTKNGRDSPGQRLGVKIYGDQVAKPGAIIIRQRGTRVYPGNNVGMGKDHTLFSLID 123

Query: 121 GLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVL 180
           GLVKFEKYGPDKKKVSVYP E QPENPNSYRARKREYFR+QRERKKAR EG IV+ QLVL
Sbjct: 124 GLVKFEKYGPDKKKVSVYPYEKQPENPNSYRARKREYFRMQRERKKARAEG-IVEVQLVL 183

Query: 181 ASTD-ATETNPVC 184
           A+ D + E N  C
Sbjct: 184 AAADESPEVNADC 195

[Close]

BLAST of Lsi03G003970 vs. Swiss-Prot
Match: RL27_NITSB (50S ribosomal protein L27 OS=Nitratiruptor sp. (strain SB155-2) GN=rpmA PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.2e-29
Identity = 60/84 (71.43%), Postives = 71/84 (84.52%), Query Frame = 1


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           AHKKG GST+N RDS G+RLGVK FG +  + G+II+RQRGTK H G NVGLGKDHTIF+
Sbjct: 2   AHKKGQGSTQNNRDSAGRRLGVKKFGGEFVRAGNIIIRQRGTKVHPGNNVGLGKDHTIFA 61

Query: 109 LIDGLVKFEKYGPDKKKVSVYPRE 133
           LIDG+VKFE+YG D++KVSVYP E
Sbjct: 62  LIDGVVKFERYGKDRQKVSVYPVE 85

[Close]

BLAST of Lsi03G003970 vs. Swiss-Prot
Match: RL27_TRIEI (50S ribosomal protein L27 OS=Trichodesmium erythraeum (strain IMS101) GN=rpmA PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.3e-27
Identity = 56/82 (68.29%), Postives = 70/82 (85.37%), Query Frame = 1


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           AHKKG GST+NGRDS  QRLGVK +G QV K G+I+VRQRG+KFHAG NVG+GKD+T+F+
Sbjct: 2   AHKKGTGSTRNGRDSNSQRLGVKRYGGQVVKAGNILVRQRGSKFHAGNNVGVGKDYTLFA 61

Query: 109 LIDGLVKFEKYGPDKKKVSVYP 131
           L++G+V FE+ G  +KKVSVYP
Sbjct: 62  LVEGMVTFERKGKSRKKVSVYP 83

[Close]

BLAST of Lsi03G003970 vs. TrEMBL
Match: A0A0A0LQ66_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406670 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 1.8e-89
Identity = 171/183 (93.44%), Postives = 176/183 (96.17%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTKNG 60
           M+LNL+++F GLSLASSSSFFKGDFGSI  SPKLSVSFPN FPLTIENAHKKGAGSTKNG
Sbjct: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG
Sbjct: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATETN 180
           PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGII QPQL+LASTD  ETN
Sbjct: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN 180

Query: 181 PVC 184
           PVC
Sbjct: 181 PVC 183

[Close]

BLAST of Lsi03G003970 vs. TrEMBL
Match: W9SAM0_9ROSA (50S ribosomal protein L27 OS=Morus notabilis GN=L484_010778 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 3.0e-76
Identity = 153/191 (80.10%), Postives = 169/191 (88.48%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSLASSSS-----FFKGDFGSIQPSP-KLSVSFPNRFPLTIENAHKKGA 60
           MS NLV AF GLS++SSSS     F +G+FGS+   P KLS S P RFPL IE+AHKKGA
Sbjct: 6   MSFNLVGAFKGLSMSSSSSSSSSSFVRGNFGSLVVGPNKLSFSCPQRFPLIIESAHKKGA 65

Query: 61  GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV 120
           GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV
Sbjct: 66  GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV 125

Query: 121 KFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLAST 180
           KFEK+GPD+KK+SVYPREVQPENPNSYRARK+EYFR+QRER+KAR+EGI+ +PQLVLAS 
Sbjct: 126 KFEKFGPDRKKISVYPREVQPENPNSYRARKKEYFRMQRERRKARQEGIVPEPQLVLASA 185

Query: 181 --DATETNPVC 184
             DAT+ NPVC
Sbjct: 186 NDDATDDNPVC 196

[Close]

BLAST of Lsi03G003970 vs. TrEMBL
Match: A5BCW1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010521 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 4.8e-74
Identity = 147/184 (79.89%), Postives = 165/184 (89.67%), Query Frame = 1


Query: 2   SLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNR-FPLTIENAHKKGAGSTKNG 61
           SLNLV AF GLSL+S+SSF +GD GSI  S KL VSFP +  PLTIE+AHKKGAGSTKNG
Sbjct: 3   SLNLVGAFKGLSLSSTSSFLRGDLGSITGSSKLCVSFPLKSLPLTIESAHKKGAGSTKNG 62

Query: 62  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 121
           RDS+GQRLGVKI+GDQVAKPG+IIVRQRGTKFH GKNVGLGKDHTIFSLIDGLVKFEK+G
Sbjct: 63  RDSRGQRLGVKIYGDQVAKPGAIIVRQRGTKFHPGKNVGLGKDHTIFSLIDGLVKFEKFG 122

Query: 122 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLAS-TDATET 181
           PD+KK+SVYP  +QPENPNS RA+KREYFR+QRERKKARKEGI+ QPQLVLAS  +AT+ 
Sbjct: 123 PDRKKISVYPXVLQPENPNSCRAKKREYFRMQRERKKARKEGIVPQPQLVLASANEATDG 182

Query: 182 NPVC 184
           NP+C
Sbjct: 183 NPIC 186

[Close]

BLAST of Lsi03G003970 vs. TrEMBL
Match: M5WZ86_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011695mg PE=3 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 6.3e-74
Identity = 155/195 (79.49%), Postives = 163/195 (83.59%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSL---------ASSSSFFKGDFGSIQPSPKLS-VSFPNRFPLTIENAH 60
           MS NLV AF GLSL         +SSSSFF+GD GS+Q  PKLS VS P R  LTI+NAH
Sbjct: 6   MSFNLVGAFRGLSLRSGSSSSSSSSSSSFFRGDLGSLQVGPKLSLVSNPKRLALTIQNAH 65

Query: 61  KKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLI 120
           KKGAGSTKNGRDS GQRLGVKIFGDQ AK GSIIVRQRGTKFHAGKNVGLGKDHTIFSLI
Sbjct: 66  KKGAGSTKNGRDSPGQRLGVKIFGDQAAKAGSIIVRQRGTKFHAGKNVGLGKDHTIFSLI 125

Query: 121 DGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIV--QPQ 180
           DGLVKFEKYGPDKKKVSVYP++VQPENPNSYRARKRE FRLQRERKKARKEG  +  QPQ
Sbjct: 126 DGLVKFEKYGPDKKKVSVYPQQVQPENPNSYRARKRENFRLQRERKKARKEGYTLRNQPQ 185

Query: 181 LVLASTDATETNPVC 184
           LVLAS DA +  PVC
Sbjct: 186 LVLASADAADVPPVC 200

[Close]

BLAST of Lsi03G003970 vs. TrEMBL
Match: D7SVP5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0068g01950 PE=3 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 8.2e-74
Identity = 147/184 (79.89%), Postives = 165/184 (89.67%), Query Frame = 1


Query: 2   SLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNR-FPLTIENAHKKGAGSTKNG 61
           SLNLV AF GLSL+S+SSF +GD GSI  S KL VSFP +  PLTIE+AHKKGAGSTKNG
Sbjct: 3   SLNLVGAFKGLSLSSTSSFLRGDLGSITGSSKLCVSFPLKSLPLTIESAHKKGAGSTKNG 62

Query: 62  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 121
           RDS+GQRLGVKI+GDQVAKPG+IIVRQRGTKFH GKNVGLGKDHTIFSLIDGLVKFEK+G
Sbjct: 63  RDSRGQRLGVKIYGDQVAKPGAIIVRQRGTKFHPGKNVGLGKDHTIFSLIDGLVKFEKFG 122

Query: 122 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLAS-TDATET 181
           PD+KK+SVYP  +QPENPNS RA+KREYFR+QRERKKARKEGI+ QPQLVLAS  +AT+ 
Sbjct: 123 PDRKKISVYPCVLQPENPNSCRAKKREYFRMQRERKKARKEGIVPQPQLVLASANEATDG 182

Query: 182 NPVC 184
           NP+C
Sbjct: 183 NPIC 186

[Close]

BLAST of Lsi03G003970 vs. TAIR10
Match: AT5G40950.1 (AT5G40950.1 ribosomal protein large subunit 27)

HSP 1 Score: 258.5 bits (659), Expect = 3.2e-69
Identity = 138/193 (71.50%), Postives = 156/193 (80.83%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNR-----FPLTIENAHKKGAG 60
           MSLNL+ AF GLSL+S+SSF +GD  S  P    +V+ P        PLTIE+AHKKGAG
Sbjct: 7   MSLNLIGAFKGLSLSSTSSFLRGDL-SFSPKTSFTVTLPLENLQAPIPLTIESAHKKGAG 66

Query: 61  STKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVK 120
           STKNGRDS GQRLGVKI+GDQVAKPG+IIVRQRGTKFHAGKNVG+GKDHTIFSLIDGLVK
Sbjct: 67  STKNGRDSPGQRLGVKIYGDQVAKPGAIIVRQRGTKFHAGKNVGIGKDHTIFSLIDGLVK 126

Query: 121 FEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG---IIVQPQLVL- 180
           FEK+GPD+KK+SVYPRE+ PENPNSYRARKRE FRLQRE+KKAR+E     +  P+LVL 
Sbjct: 127 FEKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQREKKKARRENYSYTLPTPELVLA 186

Query: 181 -ASTDATETNPVC 184
            AS D  E NP C
Sbjct: 187 SASVDDAEANPEC 198

[Close]

BLAST of Lsi03G003970 vs. TAIR10
Match: AT2G16930.2 (AT2G16930.2 Ribosomal protein L27 family protein)

HSP 1 Score: 93.6 bits (231), Expect = 1.4e-19
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 1


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           A KK AGSTKNGRDS  + LGVK FG +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 109 LIDGLVKFEK 119
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of Lsi03G003970 vs. TAIR10
Match: AT5G15220.1 (AT5G15220.1 Ribosomal protein L27 family protein)

HSP 1 Score: 93.6 bits (231), Expect = 1.4e-19
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 1


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           A KK AGSTKNGRDS  + LGVK FG +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 109 LIDGLVKFEK 119
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: gi|449441696|ref|XP_004138618.1| (PREDICTED: 50S ribosomal protein L27, chloroplastic [Cucumis sativus])

HSP 1 Score: 336.7 bits (862), Expect = 2.6e-89
Identity = 171/183 (93.44%), Postives = 176/183 (96.17%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTKNG 60
           M+LNL+++F GLSLASSSSFFKGDFGSI  SPKLSVSFPN FPLTIENAHKKGAGSTKNG
Sbjct: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG
Sbjct: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATETN 180
           PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGII QPQL+LASTD  ETN
Sbjct: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN 180

Query: 181 PVC 184
           PVC
Sbjct: 181 PVC 183

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: gi|659081447|ref|XP_008441337.1| (PREDICTED: 50S ribosomal protein L27, chloroplastic [Cucumis melo])

HSP 1 Score: 335.5 bits (859), Expect = 5.8e-89
Identity = 170/183 (92.90%), Postives = 174/183 (95.08%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSLASSSSFFKGDFGSIQPSPKLSVSFPNRFPLTIENAHKKGAGSTKNG 60
           M+LNLVA+F GLSLASSSSFFKGDFGSI   P LS+SFPN FPLTIENAHKKGAGSTKNG
Sbjct: 1   MTLNLVASFRGLSLASSSSFFKGDFGSIHTPPNLSLSFPNTFPLTIENAHKKGAGSTKNG 60

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG
Sbjct: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLASTDATETN 180
           PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGII QPQL+LASTD  ETN
Sbjct: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLILASTDDVETN 180

Query: 181 PVC 184
           PVC
Sbjct: 181 PVC 183

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: gi|703159931|ref|XP_010112373.1| (50S ribosomal protein L27 [Morus notabilis])

HSP 1 Score: 292.7 bits (748), Expect = 4.3e-76
Identity = 153/191 (80.10%), Postives = 169/191 (88.48%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSLASSSS-----FFKGDFGSIQPSP-KLSVSFPNRFPLTIENAHKKGA 60
           MS NLV AF GLS++SSSS     F +G+FGS+   P KLS S P RFPL IE+AHKKGA
Sbjct: 6   MSFNLVGAFKGLSMSSSSSSSSSSFVRGNFGSLVVGPNKLSFSCPQRFPLIIESAHKKGA 65

Query: 61  GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV 120
           GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV
Sbjct: 66  GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV 125

Query: 121 KFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQPQLVLAST 180
           KFEK+GPD+KK+SVYPREVQPENPNSYRARK+EYFR+QRER+KAR+EGI+ +PQLVLAS 
Sbjct: 126 KFEKFGPDRKKISVYPREVQPENPNSYRARKKEYFRMQRERRKARQEGIVPEPQLVLASA 185

Query: 181 --DATETNPVC 184
             DAT+ NPVC
Sbjct: 186 NDDATDDNPVC 196

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: gi|694435509|ref|XP_009344917.1| (PREDICTED: 50S ribosomal protein L27, chloroplastic [Pyrus x bretschneideri])

HSP 1 Score: 288.9 bits (738), Expect = 6.3e-75
Identity = 152/194 (78.35%), Postives = 167/194 (86.08%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSL-------ASSSSFFKGDFGSIQPSPKLSV--SFPNRFPLTIENAHK 60
           MS N+V AF GLSL       +SSSSFF+GD GS+Q  PKLS+  S P R PLTI+NAHK
Sbjct: 6   MSFNIVGAFRGLSLRPTSTSSSSSSSFFRGDLGSLQLGPKLSLVSSGPQRLPLTIQNAHK 65

Query: 61  KGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLID 120
           KGAGSTKNGRDSKGQRLGVKI+GDQVAK GSIIVRQRGTKFHAGKNVGLGKDHTIFSLID
Sbjct: 66  KGAGSTKNGRDSKGQRLGVKIYGDQVAKAGSIIVRQRGTKFHAGKNVGLGKDHTIFSLID 125

Query: 121 GLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQ--PQL 180
           G+VKFEK+GPDKKK+SVYPREVQPENPNSYR +KRE FRLQRERKKARKEG I+Q  P+L
Sbjct: 126 GMVKFEKFGPDKKKISVYPREVQPENPNSYRVKKRENFRLQRERKKARKEGYILQNKPEL 185

Query: 181 VLASTDATETNPVC 184
           +LAS DA + NPVC
Sbjct: 186 LLASADAADANPVC 199

[Close]

BLAST of Lsi03G003970 vs. NCBI nr
Match: gi|658052742|ref|XP_008362105.1| (PREDICTED: 50S ribosomal protein L27, chloroplastic-like [Malus domestica])

HSP 1 Score: 288.1 bits (736), Expect = 1.1e-74
Identity = 152/194 (78.35%), Postives = 167/194 (86.08%), Query Frame = 1


Query: 1   MSLNLVAAFTGLSL-------ASSSSFFKGDFGSIQPSPKLSV--SFPNRFPLTIENAHK 60
           MS N+V AF GLSL       +SSSSFF+GD GS+Q  PKLS+  S P R PLTI+NAHK
Sbjct: 6   MSFNVVXAFRGLSLRPTSTSSSSSSSFFRGDLGSLQLGPKLSLVSSGPQRLPLTIQNAHK 65

Query: 61  KGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLID 120
           KGAGSTKNGRDSKGQRLGVKI+GDQVAK GSIIVRQRGTKFHAGKNVGLGKDHTIFSLID
Sbjct: 66  KGAGSTKNGRDSKGQRLGVKIYGDQVAKAGSIIVRQRGTKFHAGKNVGLGKDHTIFSLID 125

Query: 121 GLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIVQ--PQL 180
           G+VKFEK+GPDKKK+SVYPREVQPENPNSYR +KRE FRLQRERKKARKEG I+Q  P+L
Sbjct: 126 GMVKFEKFGPDKKKISVYPREVQPENPNSYRVKKRENFRLQRERKKARKEGYILQNKPEL 185

Query: 181 VLASTDATETNPVC 184
           +LAS DA + NPVC
Sbjct: 186 LLASADAADANPVC 199

The following BLAST results are available for this feature:

BLAST of Lsi03G003970 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RK27_TOBAC	1.7e-72	83.83	50S ribosomal protein L27, chloroplastic OS=Nicotiana tabacum GN=RPL27 PE=1 SV=1	[more]
RK27_ARATH	5.7e-68	71.50	50S ribosomal protein L27, chloroplastic OS=Arabidopsis thaliana GN=RPL27 PE=2 S...	[more]
RK27_ORYSJ	2.0e-60	66.84	50S ribosomal protein L27, chloroplastic OS=Oryza sativa subsp. japonica GN=RPL2...	[more]
RL27_NITSB	5.2e-29	71.43	50S ribosomal protein L27 OS=Nitratiruptor sp. (strain SB155-2) GN=rpmA PE=3 SV=...	[more]
RL27_TRIEI	1.3e-27	68.29	50S ribosomal protein L27 OS=Trichodesmium erythraeum (strain IMS101) GN=rpmA PE...	[more]

BLAST of Lsi03G003970 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQ66_CUCSA	1.8e-89	93.44	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406670 PE=3 SV=1	[more]
W9SAM0_9ROSA	3.0e-76	80.10	50S ribosomal protein L27 OS=Morus notabilis GN=L484_010778 PE=3 SV=1	[more]
A5BCW1_VITVI	4.8e-74	79.89	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010521 PE=3 SV=1	[more]
M5WZ86_PRUPE	6.3e-74	79.49	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011695mg PE=3 SV=1	[more]
D7SVP5_VITVI	8.2e-74	79.89	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0068g01950 PE=3 SV=...	[more]

BLAST of Lsi03G003970 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G40950.1	3.2e-69	71.50	ribosomal protein large subunit 27	[more]
AT2G16930.2	1.4e-19	65.71	Ribosomal protein L27 family protein	[more]
AT5G15220.1	1.4e-19	65.71	Ribosomal protein L27 family protein	[more]

BLAST of Lsi03G003970 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449441696\|ref\|XP_004138618.1\|	2.6e-89	93.44	PREDICTED: 50S ribosomal protein L27, chloroplastic [Cucumis sativus]	[more]
gi\|659081447\|ref\|XP_008441337.1\|	5.8e-89	92.90	PREDICTED: 50S ribosomal protein L27, chloroplastic [Cucumis melo]	[more]
gi\|703159931\|ref\|XP_010112373.1\|	4.3e-76	80.10	50S ribosomal protein L27 [Morus notabilis]	[more]
gi\|694435509\|ref\|XP_009344917.1\|	6.3e-75	78.35	PREDICTED: 50S ribosomal protein L27, chloroplastic [Pyrus x bretschneideri]	[more]
gi\|658052742\|ref\|XP_008362105.1\|	1.1e-74	78.35	PREDICTED: 50S ribosomal protein L27, chloroplastic-like [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **Cellular Component**
Term	Definition
GO:0005840	ribosome
GO:0005622	intracellular

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome

Vocabulary: **INTERPRO**
Term	Definition
IPR018261	Ribosomal_L27_CS
IPR001684	Ribosomal_L27

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009735	response to cytokinin
biological_process	GO:0042254	ribosome biogenesis
biological_process	GO:0006412	translation
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005762	mitochondrial large ribosomal subunit
cellular_component	GO:0009579	thylakoid
cellular_component	GO:0005840	ribosome
cellular_component	GO:0044434	chloroplast part
cellular_component	GO:0005622	intracellular
molecular_function	GO:0003735	structural constituent of ribosome
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G003970.1	Lsi03G003970.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001684	Ribosomal protein L27	PRODOM	PD003114		coord: 76..116 score: 4.0
IPR001684	Ribosomal protein L27	PRINTS	PR00063	RIBOSOMALL27	coord: 51..75 score: 1.5E-39coord: 76..100 score: 1.5E-39coord: 101..125 score: 1.5
IPR001684	Ribosomal protein L27	HAMAP	MF_00539	Ribosomal_L27	coord: 48..131 score: 27
IPR001684	Ribosomal protein L27	PANTHER	PTHR15893	RIBOSOMAL PROTEIN L27	coord: 14..178 score: 7.8
IPR001684	Ribosomal protein L27	PFAM	PF01016	Ribosomal_L27	coord: 49..128 score: 5.5
IPR001684	Ribosomal protein L27	TIGRFAMs	TIGR00062	TIGR00062	coord: 48..128 score: 1.1
IPR018261	Ribosomal protein L27, conserved site	PROSITE	PS00831	RIBOSOMAL_L27	coord: 81..95 scor
None	No IPR available	unknown	Coil	Coil	coord: 141..161 scor
None	No IPR available	GENE3D	G3DSA:2.40.50.100		coord: 67..131 score: 1.2
None	No IPR available	unknown	SSF110324	Ribosomal L27 protein-like	coord: 49..130 score: 3.49

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G003970	Csa2G406670	Cucumber (Chinese Long) v2	culsiB115
Lsi03G003970	CSPI02G25620	Wild cucumber (PI 183967)	cpilsiB113
Lsi03G003970	Cucsa.087740	Cucumber (Gy14) v1	cgylsiB098
Lsi03G003970	CmaCh15G000760	Cucurbita maxima (Rimu)	cmalsiB276
Lsi03G003970	CmaCh04G029740	Cucurbita maxima (Rimu)	cmalsiB656
Lsi03G003970	CmoCh04G030930	Cucurbita moschata (Rifu)	cmolsiB645
Lsi03G003970	CmoCh15G000770	Cucurbita moschata (Rifu)	cmolsiB265
Lsi03G003970	Cla006506	Watermelon (97103) v1	lsiwmB277
Lsi03G003970	MELO3C008191	Melon (DHL92) v3.5.1	lsimeB259
Lsi03G003970	ClCG11G007120	Watermelon (Charleston Gray)	lsiwcgB226
Lsi03G003970	MELO3C008191.2	Melon (DHL92) v3.6.1	lsimedB272
Lsi03G003970	Cla97C11G214040	Watermelon (97103) v2	lsiwmbB222
Lsi03G003970	Bhi04G001986	Wax gourd	lsiwgoB322
Lsi03G003970	Cp4.1LG01g25780	Cucurbita pepo (Zucchini)	cpelsiB339
Lsi03G003970	Cp4.1LG13g11300	Cucurbita pepo (Zucchini)	cpelsiB143
Lsi03G003970	CsGy2G025250	Cucumber (Gy14) v2	cgyblsiB102
Lsi03G003970	Carg06048	Silver-seed gourd	carlsiB017
Lsi03G003970	Carg24279	Silver-seed gourd	carlsiB924
Lsi03G003970	CsaV3_2G034130	Cucumber (Chinese Long) v3	cuclsiB127

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None