CSPI02G25620 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G25620
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	50S ribosomal protein L27
Location	Chr2 : 21739351 .. 21741406 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGAGTGACTTGGTGCGGTTCGAGTGAGTGCAGGCTTGTGGCGACTGGCGAGCGTGGTTCTAAAGATAAGGTTTGCATTCGCTCCTTCTCCCTCTCTTTCACTTCTTCATATCCATTTCCTTTCTCAATTTTCTCGTCTTCCCTTCAGAATTCCCCATTGCAGCCATGACTTTAAACCTACTTTCTTCATTCAAAGGTCTCTCTCTCGCCTCCTCTTCCTCCTTCTTCAAAGGCGATTTCGGCTCCATTCATACCTCTCCTAAGCTCTCCGTTTCCTTCCCCAACATATTTCCTTTAACCATTGAAAACGCCCACAAGAAAGGAGCTGGTAGCACCAAGAACGGTCGTGATTCCAAGGGCCAGAGGCTCGGCGTTAAAATCTTCGGTGATCAAGTTGCCAAACCTGGTTCCATTATTGTTCGTCAGCGTGGGACTAAGGTAATTTCTCACCTCAATTCCCTATATTACATCCACTTCTATTTTCTACCCTTTCCCCCCCATTCTTTAGGGCATTCTTCAGCATACCTCCAACTTTTTTTTTTCTTTTGTAATTAACCCTCTCATGCCTCCATTATGAATCATTGCCATTAATAAAACTATTCTCTTTTGCCCGTGACGTAGTTAGCACATTGTTGTTGAACTACATAAGTATGTGTTGATTCAGTATTGTTTTGTATGTTGTTAAGTATGTGTTGATTCAGTATTGTTTTGTATGTTGTTACTGAGTCACCTAGATCATATAGAATCAAATTTCTTTCTGGTTCCCCCATTGTTTAGGGCACTCTTGAGCATACATCTTGTTCTGAATCATTCTCGTTCTCTCTGCCCTTGACTTAGCTAACACATAGTTGGTGAATTACTTAAATCTAGGTTGATTCTGTATTGTTTTATACACTGTTAGTCGATCAACTAAATTACGTCGAGTTGAAGTTCGTACATGTTCCTCCCATTGTTTAGTGCATTCTTGAGCATACGTCGAAGCTTCTTTGAAATTGAGTCATGATTCTGTTATCTGGGTGCGTGCTATTTACGTTATTAATCTAGAGAAGTGCACCTCCCTTCTGTGTTCAGAATTATTCAAAAAGTTCTTCCTCTACCTGGCAGAGGTAGCTAACATTGTTAGTAAACTATGTAAATCTGTGTATTTTGTATTGTTTTACATTTTTTTTTTAAATTTCTCGATTCTATAACAGTTTCTGTTTCTGTACCAATCATTTCAGTTCCATGCCGGAAAAAATGTAGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGATGGACTTGTAAAGTTCGAGAAGTATGGACCTGACAAGAAGAAGGTTTTCGCTTCGAATCTGAACCTTTCAGTATGAATCACTCTTACAAAACGAAGATATTTATTTACTTCATTGCATTGTAGGTGAGTGTTTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTATAGAGCAAGGAAGAGGGAATACTTCAGGTTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCATTATTGCTCAACCCCAATTACTCTTAGCTTCCACTGATGATGTTGAAACCAATCCAGTTTGCTGAGTTCACTTCAATCACTGCTTGTTTTAAAGCCCTGATGATCTTGAAGATTGAAGGTCTGCTTCTTTAGCTTCTCCTTTTTTTTTTTTTTTTTGTTTATGCACATATAATTTGTCAGATTCTTCACAGGAATGTGTTCCGTTATTATTCTCAATTAGCAATGTTCTACTTTTGCCCCTACGTTTTCTTTTCTCTATCATTCATTTCACTGCTGATTATTTTTTGTTGATTTTGGTGTCAAAATGGGTATTCACCAGAACAGATAATATGGGGGTGAGTTTAATTTATTGTGGTAAATGACTAGCCAAGAAATTAGATGACTTATGGTGGCTCTGCCCTGAAATTTGGTATGAATCATGCAGCATTGCCACGAGTTTATTGTTGAAATGACACTAAAATTTGATACGAATCATGTAGCTATGCCGTGAGTTTATTGTTGAAATGACACCAAGATTTGGGATTGTAATGCCCTGTTACCGG

mRNA sequence

ATGACTTTAAACCTACTTTCTTCATTCAAAGGTCTCTCTCTCGCCTCCTCTTCCTCCTTCTTCAAAGGCGATTTCGGCTCCATTCATACCTCTCCTAAGCTCTCCGTTTCCTTCCCCAACATATTTCCTTTAACCATTGAAAACGCCCACAAGAAAGGAGCTGGTAGCACCAAGAACGGTCGTGATTCCAAGGGCCAGAGGCTCGGCGTTAAAATCTTCGGTGATCAAGTTGCCAAACCTGGTTCCATTATTGTTCGTCAGCGTGGGACTAAGTTCCATGCCGGAAAAAATGTAGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGATGGACTTGTAAAGTTCGAGAAGTATGGACCTGACAAGAAGAAGGTGAGTGTTTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTATAGAGCAAGGAAGAGGGAATACTTCAGGTTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCATTATTGCTCAACCCCAATTACTCTTAGCTTCCACTGATGATGTTGAAACCAATCCAGTTTGCTGA

Coding sequence (CDS)

ATGACTTTAAACCTACTTTCTTCATTCAAAGGTCTCTCTCTCGCCTCCTCTTCCTCCTTCTTCAAAGGCGATTTCGGCTCCATTCATACCTCTCCTAAGCTCTCCGTTTCCTTCCCCAACATATTTCCTTTAACCATTGAAAACGCCCACAAGAAAGGAGCTGGTAGCACCAAGAACGGTCGTGATTCCAAGGGCCAGAGGCTCGGCGTTAAAATCTTCGGTGATCAAGTTGCCAAACCTGGTTCCATTATTGTTCGTCAGCGTGGGACTAAGTTCCATGCCGGAAAAAATGTAGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGATGGACTTGTAAAGTTCGAGAAGTATGGACCTGACAAGAAGAAGGTGAGTGTTTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTATAGAGCAAGGAAGAGGGAATACTTCAGGTTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCATTATTGCTCAACCCCAATTACTCTTAGCTTCCACTGATGATGTTGAAACCAATCCAGTTTGCTGA

Homology

[Close]

BLAST of CSPI02G25620 vs. Swiss-Prot
Match: RK27_TOBAC (50S ribosomal protein L27, chloroplastic OS=Nicotiana tabacum GN=RPL27 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 9.4e-71
Identity = 137/167 (82.04%), Postives = 151/167 (90.42%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIH-TSPKLSVSFPNIFPLTIENAHKKGAGSTKN 60
           ++ +L+ +FKGLSLASSSSF KGDFG+    +PK SVSFP   PLTIE+AHKKGAGSTKN
Sbjct: 3   VSFSLVGAFKGLSLASSSSFLKGDFGAAFPVAPKFSVSFPLKSPLTIESAHKKGAGSTKN 62

Query: 61  GRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKY 120
           GRDS GQRLGVKIFGDQVAKPGSIIVRQRGTKFH GKNVGLGKDHTIFSLIDGLVKFEK+
Sbjct: 63  GRDSPGQRLGVKIFGDQVAKPGSIIVRQRGTKFHPGKNVGLGKDHTIFSLIDGLVKFEKF 122

Query: 121 GPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQ 167
           GPD+KK+SVYPREVQPENPNSYR RKRE FRLQRER+KAR+EG+I Q
Sbjct: 123 GPDRKKISVYPREVQPENPNSYRNRKRESFRLQRERRKARREGVILQ 169

[Close]

BLAST of CSPI02G25620 vs. Swiss-Prot
Match: RK27_ARATH (50S ribosomal protein L27, chloroplastic OS=Arabidopsis thaliana GN=RPL27 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 2.6e-68
Identity = 137/192 (71.35%), Postives = 160/192 (83.33%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSSFFKGDFG-SIHTSPKLSVSFPNI---FPLTIENAHKKGAGS 60
           M+LNL+ +FKGLSL+S+SSF +GD   S  TS  +++   N+    PLTIE+AHKKGAGS
Sbjct: 7   MSLNLIGAFKGLSLSSTSSFLRGDLSFSPKTSFTVTLPLENLQAPIPLTIESAHKKGAGS 66

Query: 61  TKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKF 120
           TKNGRDS GQRLGVKI+GDQVAKPG+IIVRQRGTKFHAGKNVG+GKDHTIFSLIDGLVKF
Sbjct: 67  TKNGRDSPGQRLGVKIYGDQVAKPGAIIVRQRGTKFHAGKNVGIGKDHTIFSLIDGLVKF 126

Query: 121 EKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG---IIAQPQLLL-- 180
           EK+GPD+KK+SVYPRE+ PENPNSYRARKRE FRLQRE+KKAR+E     +  P+L+L  
Sbjct: 127 EKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQREKKKARRENYSYTLPTPELVLAS 186

Query: 181 ASTDDVETNPVC 184
           AS DD E NP C
Sbjct: 187 ASVDDAEANPEC 198

[Close]

BLAST of CSPI02G25620 vs. Swiss-Prot
Match: RK27_ORYSJ (50S ribosomal protein L27, chloroplastic OS=Oryza sativa subsp. japonica GN=RPL27 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.5e-60
Identity = 123/192 (64.06%), Postives = 145/192 (75.52%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLAS------SSSFFKGDFGSIHTSPKLSVSFPNIFP---LTIENAHK 60
           M   L+ +FKG+SL+S      S+SF + D  S+     + +  P   P   LTI+ AHK
Sbjct: 4   MAFTLVGAFKGMSLSSPCHSSSSASFLRADRVSLSVGGGVGMGVPMTMPVRRLTIQMAHK 63

Query: 61  KGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLID 120
           KGAGSTKNGRDS GQRLGVKI+GDQVAKPG+II+RQRGT+ + G NVG+GKDHT+FSLID
Sbjct: 64  KGAGSTKNGRDSPGQRLGVKIYGDQVAKPGAIIIRQRGTRVYPGNNVGMGKDHTLFSLID 123

Query: 121 GLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLL 180
           GLVKFEKYGPDKKKVSVYP E QPENPNSYRARKREYFR+QRERKKAR EGI+    +L 
Sbjct: 124 GLVKFEKYGPDKKKVSVYPYEKQPENPNSYRARKREYFRMQRERKKARAEGIVEVQLVLA 183

Query: 181 ASTDDVETNPVC 184
           A+ +  E N  C
Sbjct: 184 AADESPEVNADC 195

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BLAST of CSPI02G25620 vs. Swiss-Prot
Match: RL27_NITSB (50S ribosomal protein L27 OS=Nitratiruptor sp. (strain SB155-2) GN=rpmA PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.2e-29
Identity = 60/84 (71.43%), Postives = 71/84 (84.52%), Query Frame = 1


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           AHKKG GST+N RDS G+RLGVK FG +  + G+II+RQRGTK H G NVGLGKDHTIF+
Sbjct: 2   AHKKGQGSTQNNRDSAGRRLGVKKFGGEFVRAGNIIIRQRGTKVHPGNNVGLGKDHTIFA 61

Query: 109 LIDGLVKFEKYGPDKKKVSVYPRE 133
           LIDG+VKFE+YG D++KVSVYP E
Sbjct: 62  LIDGVVKFERYGKDRQKVSVYPVE 85

[Close]

BLAST of CSPI02G25620 vs. Swiss-Prot
Match: RL27_TRIEI (50S ribosomal protein L27 OS=Trichodesmium erythraeum (strain IMS101) GN=rpmA PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 9.9e-28
Identity = 56/82 (68.29%), Postives = 70/82 (85.37%), Query Frame = 1


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           AHKKG GST+NGRDS  QRLGVK +G QV K G+I+VRQRG+KFHAG NVG+GKD+T+F+
Sbjct: 2   AHKKGTGSTRNGRDSNSQRLGVKRYGGQVVKAGNILVRQRGSKFHAGNNVGVGKDYTLFA 61

Query: 109 LIDGLVKFEKYGPDKKKVSVYP 131
           L++G+V FE+ G  +KKVSVYP
Sbjct: 62  LVEGMVTFERKGKSRKKVSVYP 83

[Close]

BLAST of CSPI02G25620 vs. TrEMBL
Match: A0A0A0LQ66_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406670 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 1.2e-96
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60
           MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG
Sbjct: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG
Sbjct: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN 180
           PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN
Sbjct: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN 180

Query: 181 PVC 184
           PVC
Sbjct: 181 PVC 183

[Close]

BLAST of CSPI02G25620 vs. TrEMBL
Match: W9SAM0_9ROSA (50S ribosomal protein L27 OS=Morus notabilis GN=L484_010778 PE=3 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 2.8e-74
Identity = 147/191 (76.96%), Postives = 168/191 (87.96%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSS-----FFKGDFGSIHTSP-KLSVSFPNIFPLTIENAHKKGA 60
           M+ NL+ +FKGLS++SSSS     F +G+FGS+   P KLS S P  FPL IE+AHKKGA
Sbjct: 6   MSFNLVGAFKGLSMSSSSSSSSSSFVRGNFGSLVVGPNKLSFSCPQRFPLIIESAHKKGA 65

Query: 61  GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV 120
           GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV
Sbjct: 66  GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV 125

Query: 121 KFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLAST 180
           KFEK+GPD+KK+SVYPREVQPENPNSYRARK+EYFR+QRER+KAR+EGI+ +PQL+LAS 
Sbjct: 126 KFEKFGPDRKKISVYPREVQPENPNSYRARKKEYFRMQRERRKARQEGIVPEPQLVLASA 185

Query: 181 DD--VETNPVC 184
           +D   + NPVC
Sbjct: 186 NDDATDDNPVC 196

[Close]

BLAST of CSPI02G25620 vs. TrEMBL
Match: A5BCW1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010521 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.2e-72
Identity = 142/184 (77.17%), Postives = 164/184 (89.13%), Query Frame = 1


Query: 2   TLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFP-NIFPLTIENAHKKGAGSTKNG 61
           +LNL+ +FKGLSL+S+SSF +GD GSI  S KL VSFP    PLTIE+AHKKGAGSTKNG
Sbjct: 3   SLNLVGAFKGLSLSSTSSFLRGDLGSITGSSKLCVSFPLKSLPLTIESAHKKGAGSTKNG 62

Query: 62  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 121
           RDS+GQRLGVKI+GDQVAKPG+IIVRQRGTKFH GKNVGLGKDHTIFSLIDGLVKFEK+G
Sbjct: 63  RDSRGQRLGVKIYGDQVAKPGAIIVRQRGTKFHPGKNVGLGKDHTIFSLIDGLVKFEKFG 122

Query: 122 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDD-VET 181
           PD+KK+SVYP  +QPENPNS RA+KREYFR+QRERKKARKEGI+ QPQL+LAS ++  + 
Sbjct: 123 PDRKKISVYPXVLQPENPNSCRAKKREYFRMQRERKKARKEGIVPQPQLVLASANEATDG 182

Query: 182 NPVC 184
           NP+C
Sbjct: 183 NPIC 186

[Close]

BLAST of CSPI02G25620 vs. TrEMBL
Match: I1JWI0_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_04G139200 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 2.0e-72
Identity = 143/184 (77.72%), Postives = 159/184 (86.41%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60
           M+ NL + FKGLSL+SSSSFF G  GS+   P   VS P   PLTIENAHKKGAGSTKNG
Sbjct: 8   MSFNLATEFKGLSLSSSSSFFGGSSGSLRAGPASVVSLPRRCPLTIENAHKKGAGSTKNG 67

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDS+G+RLGVKI+GDQVAKPGSIIVRQRGTKFHAGKNVGLGKD+TIFSLIDG+VKFEKYG
Sbjct: 68  RDSQGKRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDYTIFSLIDGVVKFEKYG 127

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVE-T 180
           PD+KKVSVYPREVQPENPNSYRARKREYFR++RERKK R+EG I + QL+LAS DD   T
Sbjct: 128 PDRKKVSVYPREVQPENPNSYRARKREYFRVRRERKKVRQEGAILKSQLVLASADDAAIT 187

Query: 181 NPVC 184
           N +C
Sbjct: 188 NSMC 191

[Close]

BLAST of CSPI02G25620 vs. TrEMBL
Match: D7SVP5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0068g01950 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 2.0e-72
Identity = 142/184 (77.17%), Postives = 164/184 (89.13%), Query Frame = 1


Query: 2   TLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFP-NIFPLTIENAHKKGAGSTKNG 61
           +LNL+ +FKGLSL+S+SSF +GD GSI  S KL VSFP    PLTIE+AHKKGAGSTKNG
Sbjct: 3   SLNLVGAFKGLSLSSTSSFLRGDLGSITGSSKLCVSFPLKSLPLTIESAHKKGAGSTKNG 62

Query: 62  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 121
           RDS+GQRLGVKI+GDQVAKPG+IIVRQRGTKFH GKNVGLGKDHTIFSLIDGLVKFEK+G
Sbjct: 63  RDSRGQRLGVKIYGDQVAKPGAIIVRQRGTKFHPGKNVGLGKDHTIFSLIDGLVKFEKFG 122

Query: 122 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDD-VET 181
           PD+KK+SVYP  +QPENPNS RA+KREYFR+QRERKKARKEGI+ QPQL+LAS ++  + 
Sbjct: 123 PDRKKISVYPCVLQPENPNSCRAKKREYFRMQRERKKARKEGIVPQPQLVLASANEATDG 182

Query: 182 NPVC 184
           NP+C
Sbjct: 183 NPIC 186

[Close]

BLAST of CSPI02G25620 vs. TAIR10
Match: AT5G40950.1 (AT5G40950.1 ribosomal protein large subunit 27)

HSP 1 Score: 259.6 bits (662), Expect = 1.4e-69
Identity = 137/192 (71.35%), Postives = 160/192 (83.33%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSSFFKGDFG-SIHTSPKLSVSFPNI---FPLTIENAHKKGAGS 60
           M+LNL+ +FKGLSL+S+SSF +GD   S  TS  +++   N+    PLTIE+AHKKGAGS
Sbjct: 7   MSLNLIGAFKGLSLSSTSSFLRGDLSFSPKTSFTVTLPLENLQAPIPLTIESAHKKGAGS 66

Query: 61  TKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKF 120
           TKNGRDS GQRLGVKI+GDQVAKPG+IIVRQRGTKFHAGKNVG+GKDHTIFSLIDGLVKF
Sbjct: 67  TKNGRDSPGQRLGVKIYGDQVAKPGAIIVRQRGTKFHAGKNVGIGKDHTIFSLIDGLVKF 126

Query: 121 EKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG---IIAQPQLLL-- 180
           EK+GPD+KK+SVYPRE+ PENPNSYRARKRE FRLQRE+KKAR+E     +  P+L+L  
Sbjct: 127 EKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQREKKKARRENYSYTLPTPELVLAS 186

Query: 181 ASTDDVETNPVC 184
           AS DD E NP C
Sbjct: 187 ASVDDAEANPEC 198

[Close]

BLAST of CSPI02G25620 vs. TAIR10
Match: AT2G16930.2 (AT2G16930.2 Ribosomal protein L27 family protein)

HSP 1 Score: 94.0 bits (232), Expect = 1.1e-19
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 1


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           A KK AGSTKNGRDS  + LGVK FG +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 109 LIDGLVKFEK 119
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of CSPI02G25620 vs. TAIR10
Match: AT5G15220.1 (AT5G15220.1 Ribosomal protein L27 family protein)

HSP 1 Score: 94.0 bits (232), Expect = 1.1e-19
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 1


Query: 49  AHKKGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 108
           A KK AGSTKNGRDS  + LGVK FG +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 109 LIDGLVKFEK 119
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of CSPI02G25620 vs. NCBI nr
Match: gi|449441696|ref|XP_004138618.1| (PREDICTED: 50S ribosomal protein L27, chloroplastic [Cucumis sativus])

HSP 1 Score: 360.5 bits (924), Expect = 1.7e-96
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60
           MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG
Sbjct: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG
Sbjct: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN 180
           PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN
Sbjct: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN 180

Query: 181 PVC 184
           PVC
Sbjct: 181 PVC 183

[Close]

BLAST of CSPI02G25620 vs. NCBI nr
Match: gi|659081447|ref|XP_008441337.1| (PREDICTED: 50S ribosomal protein L27, chloroplastic [Cucumis melo])

HSP 1 Score: 349.4 bits (895), Expect = 3.9e-93
Identity = 175/183 (95.63%), Postives = 180/183 (98.36%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60
           MTLNL++SF+GLSLASSSSFFKGDFGSIHT P LS+SFPN FPLTIENAHKKGAGSTKNG
Sbjct: 1   MTLNLVASFRGLSLASSSSFFKGDFGSIHTPPNLSLSFPNTFPLTIENAHKKGAGSTKNG 60

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG
Sbjct: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDVETN 180
           PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQL+LASTDDVETN
Sbjct: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLILASTDDVETN 180

Query: 181 PVC 184
           PVC
Sbjct: 181 PVC 183

[Close]

BLAST of CSPI02G25620 vs. NCBI nr
Match: gi|703159931|ref|XP_010112373.1| (50S ribosomal protein L27 [Morus notabilis])

HSP 1 Score: 286.2 bits (731), Expect = 4.1e-74
Identity = 147/191 (76.96%), Postives = 168/191 (87.96%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSS-----FFKGDFGSIHTSP-KLSVSFPNIFPLTIENAHKKGA 60
           M+ NL+ +FKGLS++SSSS     F +G+FGS+   P KLS S P  FPL IE+AHKKGA
Sbjct: 6   MSFNLVGAFKGLSMSSSSSSSSSSFVRGNFGSLVVGPNKLSFSCPQRFPLIIESAHKKGA 65

Query: 61  GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV 120
           GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV
Sbjct: 66  GSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLV 125

Query: 121 KFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLAST 180
           KFEK+GPD+KK+SVYPREVQPENPNSYRARK+EYFR+QRER+KAR+EGI+ +PQL+LAS 
Sbjct: 126 KFEKFGPDRKKISVYPREVQPENPNSYRARKKEYFRMQRERRKARQEGIVPEPQLVLASA 185

Query: 181 DD--VETNPVC 184
           +D   + NPVC
Sbjct: 186 NDDATDDNPVC 196

[Close]

BLAST of CSPI02G25620 vs. NCBI nr
Match: gi|719983240|ref|XP_010250698.1| (PREDICTED: 50S ribosomal protein L27, chloroplastic [Nelumbo nucifera])

HSP 1 Score: 282.7 bits (722), Expect = 4.5e-73
Identity = 141/184 (76.63%), Postives = 163/184 (88.59%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSLASSSSFFKGDFGSIHTSPKLSVSFPNIFPLTIENAHKKGAGSTKNG 60
           M+ NL+ +F+GLSL+S SSF KGDFGSI    K+SVSFP   PLTIE AHKKGAGSTKNG
Sbjct: 6   MSFNLVGAFRGLSLSSGSSFLKGDFGSICVGNKVSVSFPLKSPLTIECAHKKGAGSTKNG 65

Query: 61  RDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYG 120
           RDS+GQRLGVKI+GDQVAKPG+II+RQRGTKFH GKNVGLGKDHTI+SLIDGLVKFEK+G
Sbjct: 66  RDSRGQRLGVKIYGDQVAKPGAIIIRQRGTKFHPGKNVGLGKDHTIYSLIDGLVKFEKFG 125

Query: 121 PDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQPQLLLASTDDV-ET 180
           PD+KKVSVYPR  QPENPNSY+ARKREYFR+QRER+KARKEG++  PQL+LAS D++ E 
Sbjct: 126 PDRKKVSVYPRVEQPENPNSYKARKREYFRMQRERRKARKEGLVPPPQLVLASVDEIPEG 185

Query: 181 NPVC 184
           +P C
Sbjct: 186 SPSC 189

[Close]

BLAST of CSPI02G25620 vs. NCBI nr
Match: gi|694435509|ref|XP_009344917.1| (PREDICTED: 50S ribosomal protein L27, chloroplastic [Pyrus x bretschneideri])

HSP 1 Score: 281.6 bits (719), Expect = 1.0e-72
Identity = 147/194 (75.77%), Postives = 164/194 (84.54%), Query Frame = 1


Query: 1   MTLNLLSSFKGLSL-------ASSSSFFKGDFGSIHTSPKLSV--SFPNIFPLTIENAHK 60
           M+ N++ +F+GLSL       +SSSSFF+GD GS+   PKLS+  S P   PLTI+NAHK
Sbjct: 6   MSFNIVGAFRGLSLRPTSTSSSSSSSFFRGDLGSLQLGPKLSLVSSGPQRLPLTIQNAHK 65

Query: 61  KGAGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLID 120
           KGAGSTKNGRDSKGQRLGVKI+GDQVAK GSIIVRQRGTKFHAGKNVGLGKDHTIFSLID
Sbjct: 66  KGAGSTKNGRDSKGQRLGVKIYGDQVAKAGSIIVRQRGTKFHAGKNVGLGKDHTIFSLID 125

Query: 121 GLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGIIAQ--PQL 180
           G+VKFEK+GPDKKK+SVYPREVQPENPNSYR +KRE FRLQRERKKARKEG I Q  P+L
Sbjct: 126 GMVKFEKFGPDKKKISVYPREVQPENPNSYRVKKRENFRLQRERKKARKEGYILQNKPEL 185

Query: 181 LLASTDDVETNPVC 184
           LLAS D  + NPVC
Sbjct: 186 LLASADAADANPVC 199

The following BLAST results are available for this feature:

BLAST of CSPI02G25620 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RK27_TOBAC	9.4e-71	82.04	50S ribosomal protein L27, chloroplastic OS=Nicotiana tabacum GN=RPL27 PE=1 SV=1	[more]
RK27_ARATH	2.6e-68	71.35	50S ribosomal protein L27, chloroplastic OS=Arabidopsis thaliana GN=RPL27 PE=2 S...	[more]
RK27_ORYSJ	1.5e-60	64.06	50S ribosomal protein L27, chloroplastic OS=Oryza sativa subsp. japonica GN=RPL2...	[more]
RL27_NITSB	5.2e-29	71.43	50S ribosomal protein L27 OS=Nitratiruptor sp. (strain SB155-2) GN=rpmA PE=3 SV=...	[more]
RL27_TRIEI	9.9e-28	68.29	50S ribosomal protein L27 OS=Trichodesmium erythraeum (strain IMS101) GN=rpmA PE...	[more]

BLAST of CSPI02G25620 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQ66_CUCSA	1.2e-96	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406670 PE=3 SV=1	[more]
W9SAM0_9ROSA	2.8e-74	76.96	50S ribosomal protein L27 OS=Morus notabilis GN=L484_010778 PE=3 SV=1	[more]
A5BCW1_VITVI	1.2e-72	77.17	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010521 PE=3 SV=1	[more]
I1JWI0_SOYBN	2.0e-72	77.72	Uncharacterized protein OS=Glycine max GN=GLYMA_04G139200 PE=3 SV=1	[more]
D7SVP5_VITVI	2.0e-72	77.17	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0068g01950 PE=3 SV=...	[more]

BLAST of CSPI02G25620 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G40950.1	1.4e-69	71.35	ribosomal protein large subunit 27	[more]
AT2G16930.2	1.1e-19	65.71	Ribosomal protein L27 family protein	[more]
AT5G15220.1	1.1e-19	65.71	Ribosomal protein L27 family protein	[more]

BLAST of CSPI02G25620 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449441696\|ref\|XP_004138618.1\|	1.7e-96	100.00	PREDICTED: 50S ribosomal protein L27, chloroplastic [Cucumis sativus]	[more]
gi\|659081447\|ref\|XP_008441337.1\|	3.9e-93	95.63	PREDICTED: 50S ribosomal protein L27, chloroplastic [Cucumis melo]	[more]
gi\|703159931\|ref\|XP_010112373.1\|	4.1e-74	76.96	50S ribosomal protein L27 [Morus notabilis]	[more]
gi\|719983240\|ref\|XP_010250698.1\|	4.5e-73	76.63	PREDICTED: 50S ribosomal protein L27, chloroplastic [Nelumbo nucifera]	[more]
gi\|694435509\|ref\|XP_009344917.1\|	1.0e-72	75.77	PREDICTED: 50S ribosomal protein L27, chloroplastic [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001684	Ribosomal_L27
IPR018261	Ribosomal_L27_CS
IPR001684	Ribosomal_L27
IPR018261	Ribosomal_L27_CS

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome
GO:0003735	structural constituent of ribosome

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular
GO:0005840	ribosome
GO:0005622	intracellular
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation
GO:0006412	translation

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042254	ribosome biogenesis
biological_process	GO:0006412	translation
biological_process	GO:0009735	response to cytokinin
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005840	ribosome
cellular_component	GO:0009579	thylakoid
cellular_component	GO:0005622	intracellular
cellular_component	GO:0044434	chloroplast part
molecular_function	GO:0003735	structural constituent of ribosome

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G25620.1	CSPI02G25620.1	mRNA
CSPI02G25620.2	CSPI02G25620.2	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001684	Ribosomal protein L27	PRODOM	PD003114		coord: 76..116 score: 4.0
IPR001684	Ribosomal protein L27	PRINTS	PR00063	RIBOSOMALL27	coord: 51..75 score: 1.2E-39coord: 101..125 score: 1.2E-39coord: 76..100 score: 1.2
IPR001684	Ribosomal protein L27	HAMAP	MF_00539	Ribosomal_L27	coord: 48..131 score: 26
IPR001684	Ribosomal protein L27	PANTHER	PTHR15893	RIBOSOMAL PROTEIN L27	coord: 14..126 score: 4.7
IPR001684	Ribosomal protein L27	PFAM	PF01016	Ribosomal_L27	coord: 49..125 score: 8.3
IPR001684	Ribosomal protein L27	TIGRFAMs	TIGR00062	TIGR00062	coord: 48..126 score: 2.9
IPR018261	Ribosomal protein L27, conserved site	PROSITE	PS00831	RIBOSOMAL_L27	coord: 81..95 scor
None	No IPR available	GENE3D	G3DSA:2.40.50.100		coord: 67..127 score: 3.6
None	No IPR available	unknown	SSF110324	Ribosomal L27 protein-like	coord: 49..127 score: 1.29

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G25620	Cla006506	Watermelon (97103) v1	cpiwmB161
CSPI02G25620	Csa2G406670	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G25620	MELO3C008191	Melon (DHL92) v3.5.1	cpimeB116
CSPI02G25620	ClCG11G007120	Watermelon (Charleston Gray)	cpiwcgB113
CSPI02G25620	Lsi03G003970	Bottle gourd (USVL1VR-Ls)	cpilsiB113
CSPI02G25620	MELO3C008191.2	Melon (DHL92) v3.6.1	cpimedB112
CSPI02G25620	CsaV3_2G034130	Cucumber (Chinese Long) v3	cpicucB081
CSPI02G25620	Cla97C11G214040	Watermelon (97103) v2	cpiwmbB114
CSPI02G25620	Bhi04G001986	Wax gourd	cpiwgoB161
CSPI02G25620	Cucsa.087740	Cucumber (Gy14) v1	cgycpiB093
CSPI02G25620	CmaCh15G000760	Cucurbita maxima (Rimu)	cmacpiB281
CSPI02G25620	CmoCh15G000770	Cucurbita moschata (Rifu)	cmocpiB271
CSPI02G25620	Cp4.1LG01g25780	Cucurbita pepo (Zucchini)	cpecpiB385
CSPI02G25620	Cp4.1LG13g11300	Cucurbita pepo (Zucchini)	cpecpiB168
CSPI02G25620	CsGy2G025250	Cucumber (Gy14) v2	cgybcpiB057
CSPI02G25620	Carg24279	Silver-seed gourd	carcpiB1048
CSPI02G25620	Carg06048	Silver-seed gourd	carcpiB0018

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G25620	Cucurbita pepo (Zucchini)	cpecpiB707
CSPI02G25620	Cucurbita maxima (Rimu)	cmacpiB702
CSPI02G25620	Cucurbita moschata (Rifu)	cmocpiB695