Cucsa.306820 (gene) Cucumber (Gy14) v1

NameCucsa.306820
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPentatricopeptide repeat-containing protein family
Locationscaffold02977 : 234484 .. 237775 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAAGCAGGCGATTGCCGCGAACATTGCGAAGCTAATTCTGAAATCTGGCCTTCAACCCTTCAAAACCACCCCATCGCTGCTTTCAAATTTTGATTCGCGGGTAATGCAATTGGTTTTATCTGATCCAAATCTTCCTACTCGGTCCTGTTTGCGGTTTTTCGACTTTCTCCGACAAAACCCATCTCGTAAACCCGATCTTCCGGCGCATTTAATCCTCTTCTCTAGGTTGTATCGAGCTCGGAAGTTCGCGGAAATGAAAAATGTGTTGAAATTCATCGTTAATGATGGAAATCTTTGGAGTAATGTTGAGCGGATTGTTtCTTCGATTGGAGGTGAGTTTAATGAGCCGAGCATCGTTGAAAAATTTTGTGATATGTTGTTTAGAGTATACATGGATAACAGAATGTTTGATTCGTCTTTGGAGGTTTTTGATTATGCGAGAAAGAAAGGGTTTGAGATTGACGAGAGATCATGTTTTGAGTTTCTACTTGCTTTGAAGAGATCTGGTAATATGGAATTATGTGTAGAATTCTTGCGTCAAATGGTCGATTCGGGCATAGAAATACGTGTTTGTTCGTGGACGGCTGTGGTTGATGGGTTGTGTAAGAAAGGGGAGGTTGTAAGGGCTAAAGCTTTGATGGATGAACTTGTCTGTAAAGGATTTAAGCCCAGTGTTATAACATATAATACTCTTTTGAATGGTTATATTGAAATTAAGGATGTGGGAGGTGTTAATGAGATTCTTAGTTTGATGGAGAAGAATGTTGTGGATTATAATGTAACAACATATACAATGTTGATTGAATGGTATTCAAGAAGTTCGAAAATTGAGGAAGCAGAGAAGCTGTTTGATGAAATGCTTAAGAAAGGAATAGAGCCTGATGTGTATATTTACACCTCCATTATCAATTGGAATTGTAAATTTGGGAACATGAAGAGGGCCTTTGTTCTGTTTGATGAAATGACTGAGAGAAGGCTTGTTCCAAATGCATACACTTATGGTGCCCTTATAAATGGTGCCTGCAAGGCAGGGGAGATGAAGGCAGCTGAGATGATGGTAAATGACATGCAAAGCAAAGGGGTTGATGTAAATCGAGTGATATTCAATACATTGATGGATGGGTACTGCAAAAAaGGAaTGATTGACGAAGCTCTAAGGCTGCAGAATATCATGCAGCAAAAAGGATTTGAGATTGATGCATTTACTTGTAACATAATTGCCAGTGGATTTTGTAGATCGAATCGGCGAGAGGAAGCAAAGAGACTATTGCTTACAATGGAAGAGAGAGGAGTGGCTCCAAATGTAGTGAGCTTCAGTATTTTGATTGACATTTACTGCAAAGAACAAAATTTTGCAGAAGCAAGAAGGCTGTTCAAGGTAATGGAGAAGAAGGGGAAGGCACCTAGTGTTGTTACGTACAATGCTTTCATTGAAAGATATTGCAAGAAGGGAAAGATGGAGGAGGCATACAAACTGATAAATGAGATGCAAGAAAGGGGGTTAATGCCGGACACATATACATATACATCACTTATAGATGGGGAACGTGCTAGTGGGAATGTGGATAGAGCGCTTGAACTATTCAATGAAATGCCACAACTAGGACTAAATCGAAATGTAGTAACTTACACGGTAATAATCTCTGGGTTGTCCAAGGATGGTAGAGCTGATGAAGCTTTTAAATTATATGATGAAATGAACAAAGAAGGCATTGTACCTGATGACGGAATATATTCTTCCTTGATAGCGAGCCTTCATAAG

mRNA sequence

ATGTCGAAGCAGGCGATTGCCGCGAACATTGCGAAGCTAATTCTGAAATCTGGCCTTCAACCCTTCAAAACCACCCCATCGCTGCTTTCAAATTTTGATTCGCGGGTAATGCAATTGGTTTTATCTGATCCAAATCTTCCTACTCGGTCCTGTTTGCGGTTTTTCGACTTTCTCCGACAAAACCCATCTCGTAAACCCGATCTTCCGGCGCATTTAATCCTCTTCTCTAGGTTGTATCGAGCTCGGAAGTTCGCGGAAATGAAAAATGTGTTGAAATTCATCGTTAATGATGGAAATCTTTGGAGTAATGTTGAGCGGATTGTTTCTTCGATTGGAGGTGAGTTTAATGAGCCGAGCATCGTTGAAAAATTTTGTGATATGTTGTTTAGAGTATACATGGATAACAGAATGTTTGATTCGTCTTTGGAGGTTTTTGATTATGCGAGAAAGAAAGGGTTTGAGATTGACGAGAGATCATGTTTTGAGTTTCTACTTGCTTTGAAGAGATCTGGTAATATGGAATTATGTGTAGAATTCTTGCGTCAAATGGTCGATTCGGGCATAGAAATACGTGTTTGTTCGTGGACGGCTGTGGTTGATGGGTTGTGTAAGAAAGGGGAGGTTGTAAGGGCTAAAGCTTTGATGGATGAACTTGTCTGTAAAGGATTTAAGCCCAGTGTTATAACATATAATACTCTTTTGAATGGTTATATTGAAATTAAGGATGTGGGAGGTGTTAATGAGATTCTTAGTTTGATGGAGAAGAATGTTGTGGATTATAATGTAACAACATATACAATGTTGATTGAATGGTATTCAAGAAGTTCGAAAATTGAGGAAGCAGAGAAGCTGTTTGATGAAATGCTTAAGAAAGGAATAGAGCCTGATGTGTATATTTACACCTCCATTATCAATTGGAATTGTAAATTTGGGAACATGAAGAGGGCCTTTGTTCTGTTTGATGAAATGACTGAGAGAAGGCTTGTTCCAAATGCATACACTTATGGTGCCCTTATAAATGGTGCCTGCAAGGCAGGGGAGATGAAGGCAGCTGAGATGATGGTAAATGACATGCAAAGCAAAGGGGTTGATGTAAATCGAGTGATATTCAATACATTGATGGATGGGTACTGCAAAAAAGGAATGATTGACGAAGCTCTAAGGCTGCAGAATATCATGCAGCAAAAAGGATTTGAGATTGATGCATTTACTTGTAACATAATTGCCAGTGGATTTTGTAGATCGAATCGGCGAGAGGAAGCAAAGAGACTATTGCTTACAATGGAAGAGAGAGGAGTGGCTCCAAATGTAGTGAGCTTCAGTATTTTGATTGACATTTACTGCAAAGAACAAAATTTTGCAGAAGCAAGAAGGCTGTTCAAGGTAATGGAGAAGAAGGGGAAGGCACCTAGTGTTGTTACGTACAATGCTTTCATTGAAAGATATTGCAAGAAGGGAAAGATGGAGGAGGCATACAAACTGATAAATGAGATGCAAGAAAGGGGGTTAATGCCGGACACATATACATATACATCACTTATAGATGGGGAACGTGCTAGTGGGAATGTGGATAGAGCGCTTGAACTATTCAATGAAATGCCACAACTAGGACTAAATCGAAATGTAGTAACTTACACGGTAATAATCTCTGGGTTGTCCAAGGATGGTAGAGCTGATGAAGCTTTTAAATTATATGATGAAATGAACAAAGAAGGCATTGTACCTGATGACGGAATATATTCTTCCTTGATAGCGAGCCTTCATAAG

Coding sequence (CDS)

ATGTCGAAGCAGGCGATTGCCGCGAACATTGCGAAGCTAATTCTGAAATCTGGCCTTCAACCCTTCAAAACCACCCCATCGCTGCTTTCAAATTTTGATTCGCGGGTAATGCAATTGGTTTTATCTGATCCAAATCTTCCTACTCGGTCCTGTTTGCGGTTTTTCGACTTTCTCCGACAAAACCCATCTCGTAAACCCGATCTTCCGGCGCATTTAATCCTCTTCTCTAGGTTGTATCGAGCTCGGAAGTTCGCGGAAATGAAAAATGTGTTGAAATTCATCGTTAATGATGGAAATCTTTGGAGTAATGTTGAGCGGATTGTTtCTTCGATTGGAGGTGAGTTTAATGAGCCGAGCATCGTTGAAAAATTTTGTGATATGTTGTTTAGAGTATACATGGATAACAGAATGTTTGATTCGTCTTTGGAGGTTTTTGATTATGCGAGAAAGAAAGGGTTTGAGATTGACGAGAGATCATGTTTTGAGTTTCTACTTGCTTTGAAGAGATCTGGTAATATGGAATTATGTGTAGAATTCTTGCGTCAAATGGTCGATTCGGGCATAGAAATACGTGTTTGTTCGTGGACGGCTGTGGTTGATGGGTTGTGTAAGAAAGGGGAGGTTGTAAGGGCTAAAGCTTTGATGGATGAACTTGTCTGTAAAGGATTTAAGCCCAGTGTTATAACATATAATACTCTTTTGAATGGTTATATTGAAATTAAGGATGTGGGAGGTGTTAATGAGATTCTTAGTTTGATGGAGAAGAATGTTGTGGATTATAATGTAACAACATATACAATGTTGATTGAATGGTATTCAAGAAGTTCGAAAATTGAGGAAGCAGAGAAGCTGTTTGATGAAATGCTTAAGAAAGGAATAGAGCCTGATGTGTATATTTACACCTCCATTATCAATTGGAATTGTAAATTTGGGAACATGAAGAGGGCCTTTGTTCTGTTTGATGAAATGACTGAGAGAAGGCTTGTTCCAAATGCATACACTTATGGTGCCCTTATAAATGGTGCCTGCAAGGCAGGGGAGATGAAGGCAGCTGAGATGATGGTAAATGACATGCAAAGCAAAGGGGTTGATGTAAATCGAGTGATATTCAATACATTGATGGATGGGTACTGCAAAAAaGGAaTGATTGACGAAGCTCTAAGGCTGCAGAATATCATGCAGCAAAAAGGATTTGAGATTGATGCATTTACTTGTAACATAATTGCCAGTGGATTTTGTAGATCGAATCGGCGAGAGGAAGCAAAGAGACTATTGCTTACAATGGAAGAGAGAGGAGTGGCTCCAAATGTAGTGAGCTTCAGTATTTTGATTGACATTTACTGCAAAGAACAAAATTTTGCAGAAGCAAGAAGGCTGTTCAAGGTAATGGAGAAGAAGGGGAAGGCACCTAGTGTTGTTACGTACAATGCTTTCATTGAAAGATATTGCAAGAAGGGAAAGATGGAGGAGGCATACAAACTGATAAATGAGATGCAAGAAAGGGGGTTAATGCCGGACACATATACATATACATCACTTATAGATGGGGAACGTGCTAGTGGGAATGTGGATAGAGCGCTTGAACTATTCAATGAAATGCCACAACTAGGACTAAATCGAAATGTAGTAACTTACACGGTAATAATCTCTGGGTTGTCCAAGGATGGTAGAGCTGATGAAGCTTTTAAATTATATGATGAAATGAACAAAGAAGGCATTGTACCTGATGACGGAATATATTCTTCCTTGATAGCGAGCCTTCATAAG

Protein sequence

MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK
BLAST of Cucsa.306820 vs. Swiss-Prot
Match: PP180_ARATH (Pentatricopeptide repeat-containing protein At2g32630 OS=Arabidopsis thaliana GN=At2g32630 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 5.0e-174
Identity = 312/592 (52.70%), Postives = 425/592 (71.79%), Query Frame = 1

Query: 2   SKQAIAANI-AKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 61
           S Q  A  I A L+ KS +   ++ PSLL N +S V +LVLS+P LPT+SC+ FF  LR+
Sbjct: 29  SDQEAARRITACLVEKSTIGKLQSNPSLLFNLNSNVTRLVLSEPTLPTQSCIDFFKLLRE 88

Query: 62  NPSR-KPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSS-IGGEFNEP 121
             S  KPDL A + L  RLY  R+F EM+++L  +VNDG     VE + S+ +  + +E 
Sbjct: 89  FESNLKPDLTAVVTLSHRLYSNRRFNEMRSLLNSVVNDGFYKRPVEELGSAMVDCDISEE 148

Query: 122 SI--VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELC 181
                EKF D++FRVY+DN MF+  L VFDY  KKG  IDERSC  FL+A K+   ++LC
Sbjct: 149 KFEFFEKFFDLVFRVYVDNGMFEEGLRVFDYMVKKGLSIDERSCIVFLVAAKKRRRIDLC 208

Query: 182 VEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNG 241
           +E  R+MVDSG++I V S T VV+GLC++GEV ++K L+ E   KG KP   TYNT++N 
Sbjct: 209 LEIFRRMVDSGVKITVYSLTIVVEGLCRRGEVEKSKKLIKEFSVKGIKPEAYTYNTIINA 268

Query: 242 YIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPD 301
           Y++ +D  GV  +L +M+K+ V YN  TYT+L+E   ++ K+ +AEKLFDEM ++GIE D
Sbjct: 269 YVKQRDFSGVEGVLKVMKKDGVVYNKVTYTLLMELSVKNGKMSDAEKLFDEMRERGIESD 328

Query: 302 VYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVN 361
           V++YTS+I+WNC+ GNMKRAF+LFDE+TE+ L P++YTYGALI+G CK GEM AAE+++N
Sbjct: 329 VHVYTSLISWNCRKGNMKRAFLLFDELTEKGLSPSSYTYGALIDGVCKVGEMGAAEILMN 388

Query: 362 DMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSN 421
           +MQSKGV++ +V+FNTL+DGYC+KGM+DEA  + ++M+QKGF+ D FTCN IAS F R  
Sbjct: 389 EMQSKGVNITQVVFNTLIDGYCRKGMVDEASMIYDVMEQKGFQADVFTCNTIASCFNRLK 448

Query: 422 RREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYN 481
           R +EAK+ L  M E GV  + VS++ LID+YCKE N  EA+RLF  M  KG  P+ +TYN
Sbjct: 449 RYDEAKQWLFRMMEGGVKLSTVSYTNLIDVYCKEGNVEEAKRLFVEMSSKGVQPNAITYN 508

Query: 482 AFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQL 541
             I  YCK+GK++EA KL   M+  G+ PD+YTYTSLI GE  + NVD A+ LF+EM   
Sbjct: 509 VMIYAYCKQGKIKEARKLRANMEANGMDPDSYTYTSLIHGECIADNVDEAMRLFSEMGLK 568

Query: 542 GLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
           GL++N VTYTV+ISGLSK G++DEAF LYDEM ++G   D+ +Y++LI S+H
Sbjct: 569 GLDQNSVTYTVMISGLSKAGKSDEAFGLYDEMKRKGYTIDNKVYTALIGSMH 620

BLAST of Cucsa.306820 vs. Swiss-Prot
Match: PP407_ARATH (Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana GN=EMB2745 PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.6e-71
Identity = 150/460 (32.61%), Postives = 259/460 (56.30%), Query Frame = 1

Query: 126 DMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSG-NMELCVEFLRQMV 185
           D++ + Y    + D +L +   A+  GF     S    L A  RS  N+       ++M+
Sbjct: 138 DLVVKSYSRLSLIDKALSIVHLAQAHGFMPGVLSYNAVLDATIRSKRNISFAENVFKEML 197

Query: 186 DSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVG 245
           +S +   V ++  ++ G C  G +  A  L D++  KG  P+V+TYNTL++GY +++ + 
Sbjct: 198 ESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKID 257

Query: 246 GVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSII 305
              ++L  M    ++ N+ +Y ++I    R  +++E   +  EM ++G   D   Y ++I
Sbjct: 258 DGFKLLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLI 317

Query: 306 NWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVD 365
              CK GN  +A V+  EM    L P+  TY +LI+  CKAG M  A   ++ M+ +G+ 
Sbjct: 318 KGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLC 377

Query: 366 VNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRL 425
            N   + TL+DG+ +KG ++EA R+   M   GF     T N + +G C + + E+A  +
Sbjct: 378 PNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAV 437

Query: 426 LLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCK 485
           L  M+E+G++P+VVS+S ++  +C+  +  EA R+ + M +KG  P  +TY++ I+ +C+
Sbjct: 438 LEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCE 497

Query: 486 KGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVT 545
           + + +EA  L  EM   GL PD +TYT+LI+     G++++AL+L NEM + G+  +VVT
Sbjct: 498 QRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVT 557

Query: 546 YTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 585
           Y+V+I+GL+K  R  EA +L  ++  E  VP D  Y +LI
Sbjct: 558 YSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLI 597


HSP 2 Score: 228.8 bits (582), Expect = 1.6e-58
Identity = 133/433 (30.72%), Postives = 225/433 (51.96%), Query Frame = 1

Query: 170 SGNMELCVEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVIT 229
           +GN+++ +    +M   G    V ++  ++DG CK  ++     L+  +  KG +P++I+
Sbjct: 218 AGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLIS 277

Query: 230 YNTLLNGYIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEML 289
           YN ++NG      +  V+ +L+ M +     +  TY  LI+ Y +     +A  +  EML
Sbjct: 278 YNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEML 337

Query: 290 KKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMK 349
           + G+ P V  YTS+I+  CK GNM RA    D+M  R L PN  TY  L++G  + G M 
Sbjct: 338 RHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMN 397

Query: 350 AAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIA 409
            A  ++ +M   G   + V +N L++G+C  G +++A+ +   M++KG   D  + + + 
Sbjct: 398 EAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVL 457

Query: 410 SGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKA 469
           SGFCRS   +EA R+   M E+G+ P+ +++S LI  +C+++   EA  L++ M + G  
Sbjct: 458 SGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLP 517

Query: 470 PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA--- 529
           P   TY A I  YC +G +E+A +L NEM E+G++PD  TY+ LI+G         A   
Sbjct: 518 PDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRL 577

Query: 530 -LELFNEMPQLGLNRNVVTYTVI--------------ISGLSKDGRADEAFKLYDEMNKE 585
            L+LF E     +  +V  +T+I              I G    G   EA ++++ M  +
Sbjct: 578 LLKLFYEE---SVPSDVTYHTLIENCSNIEFKSVVSLIKGFCMKGMMTEADQVFESMLGK 637


HSP 3 Score: 202.6 bits (514), Expect = 1.2e-50
Identity = 123/444 (27.70%), Postives = 216/444 (48.65%), Query Frame = 1

Query: 132 YMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIR 191
           Y   R  D   ++      KG E +  S    +  L R G M+     L +M   G  + 
Sbjct: 250 YCKLRKIDDGFKLLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLD 309

Query: 192 VCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILS 251
             ++  ++ G CK+G   +A  +  E++  G  PSVITY +L++   +  ++    E L 
Sbjct: 310 EVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLD 369

Query: 252 LMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFG 311
            M    +  N  TYT L++ +S+   + EA ++  EM   G  P V  Y ++IN +C  G
Sbjct: 370 QMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTG 429

Query: 312 NMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFN 371
            M+ A  + ++M E+ L P+  +Y  +++G C++ ++  A  +  +M  KG+  + + ++
Sbjct: 430 KMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYS 489

Query: 372 TLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEER 431
           +L+ G+C++    EA  L   M + G   D FT   + + +C     E+A +L   M E+
Sbjct: 490 SLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEK 549

Query: 432 GVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIER---------- 491
           GV P+VV++S+LI+   K+    EA+RL   +  +   PS VTY+  IE           
Sbjct: 550 GVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFKSVV 609

Query: 492 -----YCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQL 551
                +C KG M EA ++   M  +   PD   Y  +I G   +G++ +A  L+ EM + 
Sbjct: 610 SLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEMVKS 669

Query: 552 GLNRNVVTYTVIISGLSKDGRADE 561
           G   + VT   ++  L K+G+ +E
Sbjct: 670 GFLLHTVTVIALVKALHKEGKVNE 693


HSP 4 Score: 168.7 bits (426), Expect = 1.9e-40
Identity = 118/441 (26.76%), Postives = 214/441 (48.53%), Query Frame = 1

Query: 119 SIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVE 178
           S+ E   + L + Y     F  +L +     + G      +    + ++ ++GNM   +E
Sbjct: 307 SLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAME 366

Query: 179 FLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYI 238
           FL QM   G+     ++T +VDG  +KG +  A  ++ E+   GF PSV+TYN L+NG+ 
Sbjct: 367 FLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHC 426

Query: 239 EIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVY 298
               +     +L  M++  +  +V +Y+ ++  + RS  ++EA ++  EM++KGI+PD  
Sbjct: 427 VTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTI 486

Query: 299 IYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDM 358
            Y+S+I   C+    K A  L++EM    L P+ +TY ALIN  C  G+++ A  + N+M
Sbjct: 487 TYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEM 546

Query: 359 QSKGVDVNRVIFNTLMDGYCKKGMIDEALRL-------QNIMQQKGFEIDAFTCNII--- 418
             KGV  + V ++ L++G  K+    EA RL       +++     +      C+ I   
Sbjct: 547 VEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFK 606

Query: 419 -----ASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVM 478
                  GFC      EA ++  +M  +   P+  +++I+I  +C+  +  +A  L+K M
Sbjct: 607 SVVSLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEM 666

Query: 479 EKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNV 538
            K G     VT  A ++   K+GK+ E   +I  +     + +      L++     GN+
Sbjct: 667 VKSGFLLHTVTVIALVKALHKEGKVNELNSVIVHVLRSCELSEAEQAKVLVEINHREGNM 726

Query: 539 DRALELFNEMPQLGLNRNVVT 545
           D  L++  EM + G   N ++
Sbjct: 727 DVVLDVLAEMAKDGFLPNGIS 747

BLAST of Cucsa.306820 vs. Swiss-Prot
Match: PPR27_ARATH (Pentatricopeptide repeat-containing protein At1g09820 OS=Arabidopsis thaliana GN=At1g09820 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 7.4e-69
Identity = 169/552 (30.62%), Postives = 292/552 (52.90%), Query Frame = 1

Query: 38  QLVLS--DPNLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIV 97
           QL+ S  DP+L    CLR++ +L +N      L     L   L  A++++++++ L   V
Sbjct: 73  QLISSELDPDL----CLRYYSWLVKNSDISVSLELTFKLLHSLANAKRYSKIRSFLDGFV 132

Query: 98  NDGNLWSNVERIVSSIGGEFNEPSIVEKFC------DMLFRVYMDNRMFDSSLEVFDYAR 157
            +G+     +  V SI   F+  S+ +  C      DML   Y +N  F+   E F  + 
Sbjct: 133 RNGS-----DHQVHSI---FHAISMCDNVCVNSIIADMLVLAYANNSRFELGFEAFKRSG 192

Query: 158 KKGFEIDERSCFEFLLALKRSGNMELCVEFL-RQMVDSGIEIRVCSWTAVVDGLCKKGEV 217
             G+++   SC   ++AL +  N    VE++ ++M+   I+  V ++  V++ LCK G++
Sbjct: 193 YYGYKLSALSCKPLMIALLKE-NRSADVEYVYKEMIRRKIQPNVFTFNVVINALCKTGKM 252

Query: 218 VRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGV---NEILSLMEKNVVDYNVTTY 277
            +A+ +M+++   G  P+V++YNTL++GY ++   G +   + +L  M +N V  N+TT+
Sbjct: 253 NKARDVMEDMKVYGCSPNVVSYNTLIDGYCKLGGNGKMYKADAVLKEMVENDVSPNLTTF 312

Query: 278 TMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTE 337
            +LI+ + +   +  + K+F EML + ++P+V  Y S+IN  C  G +  A  + D+M  
Sbjct: 313 NILIDGFWKDDNLPGSMKVFKEMLDQDVKPNVISYNSLINGLCNGGKISEAISMRDKMVS 372

Query: 338 RRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDE 397
             + PN  TY ALING CK   +K A  M   ++ +G      ++N L+D YCK G ID+
Sbjct: 373 AGVQPNLITYNALINGFCKNDMLKEALDMFGSVKGQGAVPTTRMYNMLIDAYCKLGKIDD 432

Query: 398 ALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILID 457
              L+  M+++G   D  T N + +G CR+   E AK+L   +  +G+ P++V+F IL++
Sbjct: 433 GFALKEEMEREGIVPDVGTYNCLIAGLCRNGNIEAAKKLFDQLTSKGL-PDLVTFHILME 492

Query: 458 IYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQ-ERGLM 517
            YC++    +A  L K M K G  P  +TYN  ++ YCK+G ++ A  +  +M+ ER L 
Sbjct: 493 GYCRKGESRKAAMLLKEMSKMGLKPRHLTYNIVMKGYCKEGNLKAATNMRTQMEKERRLR 552

Query: 518 PDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKL 577
            +  +Y  L+ G    G ++ A  L NEM + GL  N +TY ++                
Sbjct: 553 MNVASYNVLLQGYSQKGKLEDANMLLNEMLEKGLVPNRITYEIV---------------- 594

BLAST of Cucsa.306820 vs. Swiss-Prot
Match: PP152_ARATH (Pentatricopeptide repeat-containing protein At2g15630, mitochondrial OS=Arabidopsis thaliana GN=At2g15630 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.8e-68
Identity = 146/462 (31.60%), Postives = 243/462 (52.60%), Query Frame = 1

Query: 126 DMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVD 185
           D+L R     RM D ++E F   ++KGF     +C   L  L R   +E    F   M  
Sbjct: 159 DLLVRCCCQLRMVDEAIECFYLMKEKGFYPKTETCNHILTLLSRLNRIENAWVFYADMYR 218

Query: 186 SGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGG 245
             I+  V ++  +++ LCK+G++ +AK  +  +   G KP+++TYNTL+ G+     + G
Sbjct: 219 MEIKSNVYTFNIMINVLCKEGKLKKAKGFLGIMEVFGIKPTIVTYNTLVQGFSLRGRIEG 278

Query: 246 VNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIIN 305
              I+S M+      ++ TY  ++ W     +   A ++  EM + G+ PD   Y  +I 
Sbjct: 279 ARLIISEMKSKGFQPDMQTYNPILSWMCNEGR---ASEVLREMKEIGLVPDSVSYNILIR 338

Query: 306 WNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDV 365
                G+++ AF   DEM ++ +VP  YTY  LI+G     +++AAE+++ +++ KG+ +
Sbjct: 339 GCSNNGDLEMAFAYRDEMVKQGMVPTFYTYNTLIHGLFMENKIEAAEILIREIREKGIVL 398

Query: 366 NRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLL 425
           + V +N L++GYC+ G   +A  L + M   G +   FT   +    CR N+  EA  L 
Sbjct: 399 DSVTYNILINGYCQHGDAKKAFALHDEMMTDGIQPTQFTYTSLIYVLCRKNKTREADELF 458

Query: 426 LTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKK 485
             +  +G+ P++V  + L+D +C   N   A  L K M+     P  VTYN  +   C +
Sbjct: 459 EKVVGKGMKPDLVMMNTLMDGHCAIGNMDRAFSLLKEMDMMSINPDDVTYNCLMRGLCGE 518

Query: 486 GKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTY 545
           GK EEA +L+ EM+ RG+ PD  +Y +LI G    G+   A  + +EM  LG N  ++TY
Sbjct: 519 GKFEEARELMGEMKRRGIKPDHISYNTLISGYSKKGDTKHAFMVRDEMLSLGFNPTLLTY 578

Query: 546 TVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 588
             ++ GLSK+   + A +L  EM  EGIVP+D  + S+I ++
Sbjct: 579 NALLKGLSKNQEGELAEELLREMKSEGIVPNDSSFCSVIEAM 617

BLAST of Cucsa.306820 vs. Swiss-Prot
Match: PPR26_ARATH (Putative pentatricopeptide repeat-containing protein At1g09680 OS=Arabidopsis thaliana GN=At1g09680 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-67
Identity = 150/539 (27.83%), Postives = 283/539 (52.50%), Query Frame = 1

Query: 23  KTTPSLLSNFDSRVMQLVLSDP-NLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRA 82
           K  PSL  +    V+ L+  +P +LP RS   FF F+   P  +  +  + +L   L   
Sbjct: 75  KVLPSLSVH---HVVDLINHNPLSLPQRSIFAFFKFISSQPGFRFTVETYFVLARFLAVH 134

Query: 83  RKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSIVEKFCDMLFRVYMDNRMFDSS 142
             F E +++++ +V+     S     +S +  E     +     D L   Y D      +
Sbjct: 135 EMFTEAQSLIELVVSRKGKNSASSVFISLV--EMRVTPMCGFLVDALMITYTDLGFIPDA 194

Query: 143 LEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIRVCSWTAVVDG 202
           ++ F  +RK  F++  R C   L  + +         F  +++D+G  + V  +  +++ 
Sbjct: 195 IQCFRLSRKHRFDVPIRGCGNLLDRMMKLNPTGTIWGFYMEILDAGFPLNVYVFNILMNK 254

Query: 203 LCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILSLMEKNVVDYN 262
            CK+G +  A+ + DE+  +  +P+V+++NTL+NGY ++ ++     +   MEK+    +
Sbjct: 255 FCKEGNISDAQKVFDEITKRSLQPTVVSFNTLINGYCKVGNLDEGFRLKHQMEKSRTRPD 314

Query: 263 VTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFD 322
           V TY+ LI    + +K++ A  LFDEM K+G+ P+  I+T++I+ + + G +      + 
Sbjct: 315 VFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFTTLIHGHSRNGEIDLMKESYQ 374

Query: 323 EMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKG 382
           +M  + L P+   Y  L+NG CK G++ AA  +V+ M  +G+  +++ + TL+DG+C+ G
Sbjct: 375 KMLSKGLQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRRGLRPDKITYTTLIDGFCRGG 434

Query: 383 MIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFS 442
            ++ AL ++  M Q G E+D    + +  G C+  R  +A+R L  M   G+ P+ V+++
Sbjct: 435 DVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDAERALREMLRAGIKPDDVTYT 494

Query: 443 ILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQER 502
           +++D +CK+ +     +L K M+  G  PSVVTYN  +   CK G+M+ A  L++ M   
Sbjct: 495 MMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVTYNVLLNGLCKLGQMKNADMLLDAMLNI 554

Query: 503 GLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGL---SKDGR 558
           G++PD  TY +L++G     N  +    + + P++G+  ++ +Y  I++ L   SKD R
Sbjct: 555 GVVPDDITYNTLLEGHHRHANSSKR---YIQKPEIGIVADLASYKSIVNELDRASKDHR 605


HSP 2 Score: 152.9 bits (385), Expect = 1.1e-35
Identity = 87/303 (28.71%), Postives = 152/303 (50.17%), Query Frame = 1

Query: 287 EMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAG 346
           E+L  G   +VY++  ++N  CK GN+  A  +FDE+T+R L P   ++  LING CK G
Sbjct: 230 EILDAGFPLNVYVFNILMNKFCKEGNISDAQKVFDEITKRSLQPTVVSFNTLINGYCKVG 289

Query: 347 EMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCN 406
            +     + + M+      +   ++ L++  CK+  +D A  L + M ++G   +     
Sbjct: 290 NLDEGFRLKHQMEKSRTRPDVFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFT 349

Query: 407 IIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKK 466
            +  G  R+   +  K     M  +G+ P++V ++ L++ +CK  +   AR +   M ++
Sbjct: 350 TLIHGHSRNGEIDLMKESYQKMLSKGLQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRR 409

Query: 467 GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 526
           G  P  +TY   I+ +C+ G +E A ++  EM + G+  D   +++L+ G    G V  A
Sbjct: 410 GLRPDKITYTTLIDGFCRGGDVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDA 469

Query: 527 LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIAS 586
                EM + G+  + VTYT+++    K G A   FKL  EM  +G VP    Y+ L+  
Sbjct: 470 ERALREMLRAGIKPDDVTYTMMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVTYNVLLNG 529

Query: 587 LHK 590
           L K
Sbjct: 530 LCK 532


HSP 3 Score: 121.3 bits (303), Expect = 3.5e-26
Identity = 65/241 (26.97%), Postives = 131/241 (54.36%), Query Frame = 1

Query: 344 KAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAF 403
           + G+  A+ + ++ ++ +   +   + + LM  Y   G I +A++   + ++  F++   
Sbjct: 147 RKGKNSASSVFISLVEMRVTPMCGFLVDALMITYTDLGFIPDAIQCFRLSRKHRFDVPIR 206

Query: 404 TCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVM 463
            C  +     + N         + + + G   NV  F+IL++ +CKE N ++A+++F  +
Sbjct: 207 GCGNLLDRMMKLNPTGTIWGFYMEILDAGFPLNVYVFNILMNKFCKEGNISDAQKVFDEI 266

Query: 464 EKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNV 523
            K+   P+VV++N  I  YCK G ++E ++L ++M++    PD +TY++LI+       +
Sbjct: 267 TKRSLQPTVVSFNTLINGYCKVGNLDEGFRLKHQMEKSRTRPDVFTYSALINALCKENKM 326

Query: 524 DRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSL 583
           D A  LF+EM + GL  N V +T +I G S++G  D   + Y +M  +G+ PD  +Y++L
Sbjct: 327 DGAHGLFDEMCKRGLIPNDVIFTTLIHGHSRNGEIDLMKESYQKMLSKGLQPDIVLYNTL 386

Query: 584 I 585
           +
Sbjct: 387 V 387

BLAST of Cucsa.306820 vs. TrEMBL
Match: A0A0A0LDI0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 3.1e-263
Identity = 469/528 (88.83%), Postives = 481/528 (91.10%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ
Sbjct: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60

Query: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120
           NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI
Sbjct: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120

Query: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180
           VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL
Sbjct: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180

Query: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240
           RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI
Sbjct: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240

Query: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300
           KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY
Sbjct: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300

Query: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360
           TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS
Sbjct: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360

Query: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420
           KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE
Sbjct: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420

Query: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480
           AKRLLLTMEERGVAPNVVSF    +      N   A  LF  M + G   +VVTY   I 
Sbjct: 421 AKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTVIIS 480

Query: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALE 529
              K G+ +EA+KL +EM + G++PD   Y+SLI      G +   LE
Sbjct: 481 GLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHKVGPLVSGLE 524

BLAST of Cucsa.306820 vs. TrEMBL
Match: A0A0A0LDI0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.7e-67
Identity = 142/342 (41.52%), Postives = 215/342 (62.87%), Query Frame = 1

Query: 248 EILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWN 307
           E L  M  + ++  V ++T +++   +  ++  A+ L DE++ KG +P V  Y +++N  
Sbjct: 178 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 237

Query: 308 CKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNR 367
            +  ++     +   M +  +  N  TY  LI    ++ +++ AE + ++M  KG++ + 
Sbjct: 238 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 297

Query: 368 VIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLT 427
            I+ ++++  CK G +  A  L + M ++    +A+T   + +G C++   + A+ ++  
Sbjct: 298 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 357

Query: 428 MEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGK 487
           M+ +GV  N V F+ L+D YCK+    EA RL  +M++KG      T N     +C+  +
Sbjct: 358 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 417

Query: 488 MEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTV 547
            EEA +L+  M+ERG+ P+  ++    +GERASGNVDRALELFNEMPQLGLNRNVVTYTV
Sbjct: 418 REEAKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTV 477

Query: 548 IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK
Sbjct: 478 IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 515


HSP 2 Score: 148.7 bits (374), Expect = 2.3e-32
Identity = 85/310 (27.42%), Postives = 160/310 (51.61%), Query Frame = 1

Query: 267 MLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTER 326
           ML   Y  +   + + ++FD   KKG E D       +    + GNM+       +M + 
Sbjct: 127 MLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDS 186

Query: 327 RLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEA 386
            +     ++ A+++G CK GE+  A+ +++++  KG   + + +NTL++GY +   +   
Sbjct: 187 GIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGV 246

Query: 387 LRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDI 446
             + ++M++   + +  T  ++   + RS++ EEA++L   M ++G+ P+V  ++ +I+ 
Sbjct: 247 NEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINW 306

Query: 447 YCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPD 506
            CK  N   A  LF  M ++   P+  TY A I   CK G+M+ A  ++N+MQ +G+  +
Sbjct: 307 NCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVN 366

Query: 507 TYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYD 566
              + +L+DG    G +D AL L N M Q G   +  T  +I SG  +  R +EA +L  
Sbjct: 367 RVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLL 426

Query: 567 EMNKEGIVPD 577
            M + G+ P+
Sbjct: 427 TMEERGVAPN 436


HSP 3 Score: 700.7 bits (1807), Expect = 1.6e-198
Identity = 347/589 (58.91%), Postives = 444/589 (75.38%), Query Frame = 1

Query: 4   QAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQNPS 63
           Q IA  I K+++KSGL+P +T  SLLSN DS    LVLS+PNLPTRSCL FF  L++N S
Sbjct: 24  QEIAEKITKILIKSGLKPLETKSSLLSNLDSYTTNLVLSNPNLPTRSCLNFFKCLQKNQS 83

Query: 64  ---RKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 123
               KPDL AH+IL SRL++ARKF  MKNVL     D NL  +V   VS I   F+EP  
Sbjct: 84  LICHKPDLRAHVILISRLFKARKFVVMKNVLTCYAMDKNLRCSVSDFVSLIDNRFHEPKF 143

Query: 124 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 183
           VEK CDMLFRVY+DN MF+   +VFDY    G +ID+RSC   LLALKRS  M + + F 
Sbjct: 144 VEKLCDMLFRVYVDNSMFEEGFKVFDYMVHNGLKIDDRSCIVCLLALKRSDQMVMSLGFF 203

Query: 184 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 243
           ++MV+  +++ V S T V+DGLCKKG V RAK LM E+  KG KP+V+TYNTL+N YI+I
Sbjct: 204 KKMVEFKVDVTVYSMTIVIDGLCKKGRVERAKDLMLEMTGKGIKPNVVTYNTLVNAYIKI 263

Query: 244 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 303
            D  GVNE+L LME + V YN  TYT+LIEWY  S KI EAEK+F++ML++G+E D++++
Sbjct: 264 MDFEGVNEMLRLMEMDKVVYNAATYTLLIEWYGSSGKIAEAEKVFEKMLERGVEADIHVF 323

Query: 304 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 363
           TSII+W CK GNMKRAF LFDE+ ER LV NA+TYGALI+G C +G++ AAEM+VN+MQS
Sbjct: 324 TSIISWQCKLGNMKRAFALFDELNERGLVANAHTYGALIHGTCNSGQLDAAEMLVNEMQS 383

Query: 364 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 423
           +G+D+N VIFNTL++GYCKKGMIDEALR+Q++M++KGFE D FT N IA G C+ NR EE
Sbjct: 384 QGLDMNLVIFNTLINGYCKKGMIDEALRMQDVMEKKGFENDIFTYNTIAGGLCKLNRHEE 443

Query: 424 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 483
           AKR L TM E+GV PN VSF+I+IDI+CKE N  EA R+F+ M+KKG+ P+VVTYN  I+
Sbjct: 444 AKRWLFTMVEKGVDPNAVSFTIMIDIHCKEGNLVEAERVFQDMKKKGEKPNVVTYNTLID 503

Query: 484 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR 543
            Y KKGK++EAY+L +EM+  G+  D YTYT+L+ GE   G V+ AL L NE+ + GL  
Sbjct: 504 GYSKKGKLKEAYRLKDEMESIGMTSDIYTYTTLVHGECVFGKVEEALTLLNEVCRKGLAI 563

Query: 544 NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           ++VTYT IISGLSK+GR++EAF+LYDEM   G+ PDD +Y+SL+A+LHK
Sbjct: 564 SIVTYTAIISGLSKEGRSEEAFRLYDEMMAAGLTPDDRVYTSLVANLHK 612

BLAST of Cucsa.306820 vs. TrEMBL
Match: V4S3K8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011284mg PE=4 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.7e-195
Identity = 333/588 (56.63%), Postives = 440/588 (74.83%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           +S + I+  IAK +  SG++PFKTTPSLLSN +S +  LVLSD NLP  +CL FF+FLRQ
Sbjct: 21  ISYKDISTQIAKTLFNSGIEPFKTTPSLLSNLNSHITHLVLSDTNLPPHACLHFFNFLRQ 80

Query: 61  NPS---RKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNE 120
           NPS    +PDL AH+ L  RL+ A+KFAEMK V  +IV D NL  ++  ++S I     +
Sbjct: 81  NPSLYSHRPDLQAHVTLICRLHEAQKFAEMKKVFNYIVTDANLRCSIVNLISLIESGNQQ 140

Query: 121 PSIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCV 180
           P  VEKFCD+LFRVY DNR+F+ +L VFDY  + G +IDERSC  FLLALKR  ++E+C 
Sbjct: 141 PKFVEKFCDLLFRVYADNRLFEDALGVFDYMERSGLKIDERSCIAFLLALKRCDHIEMCF 200

Query: 181 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 240
           +F R+MVD+ +EI V S + ++DGLCK+G+V  A+ LMDE+V KG KP++ TYNTLLNG+
Sbjct: 201 KFFRKMVDANVEITVYSISIMIDGLCKRGDVEMARDLMDEMVVKGIKPNLYTYNTLLNGF 260

Query: 241 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 300
           ++ KD  GVNE+LSLM+++ V YNV TYT+L +WY  S K+ E EK+FDEM ++G+E DV
Sbjct: 261 VKRKDAVGVNEMLSLMQRDQVAYNVVTYTLLTDWYGNSGKMVEVEKMFDEMRERGVEADV 320

Query: 301 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 360
           ++YT++I+WNCK GNM+RAF LFD++TER +VPN++ YGA+I+G CK G+++AAEM++ D
Sbjct: 321 HVYTTVISWNCKSGNMRRAFTLFDDLTERGIVPNSHIYGAMIDGVCKVGQVEAAEMLLKD 380

Query: 361 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 420
           MQ +G+DVN VIFNTLM+GYCKKGMIDEAL++  IM++KGFE   F  N IASG C+  R
Sbjct: 381 MQRQGLDVNEVIFNTLMNGYCKKGMIDEALKVMAIMEKKGFEAGPFAYNTIASGLCKVKR 440

Query: 421 REEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNA 480
             EAK+ L TM ERG+A N V+FS LIDI CKE N  EA+ LF+ M+ KG  P+  TYN 
Sbjct: 441 YNEAKKWLFTMMERGLALNTVNFSTLIDICCKEGNLEEAKWLFEDMKMKGVKPNSFTYNT 500

Query: 481 FIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLG 540
            I  Y KKG +++AY+L  +M+E G+ PD YTYTSLI GER  G +D A+ LFNEM + G
Sbjct: 501 LIHGYSKKGNIKQAYRLKCKMEEMGMTPDVYTYTSLIHGERVFGKLDEAMILFNEMIEKG 560

Query: 541 LNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 586
              N VTYT +I+GLSK+GR+DEAF+LYDEM   G+ PDD + SSL+A
Sbjct: 561 FIPNAVTYTTLITGLSKEGRSDEAFRLYDEMIASGLTPDDKLCSSLVA 608

BLAST of Cucsa.306820 vs. TrEMBL
Match: A0A067DGQ8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g047794mg PE=4 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 6.1e-195
Identity = 333/588 (56.63%), Postives = 439/588 (74.66%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           +S + I+  IAK +  SG++PFKTTPSLLSN +S +  LVLSD NLP  +CL FF+FLRQ
Sbjct: 21  ISYKDISTQIAKTLFNSGIEPFKTTPSLLSNLNSHITHLVLSDTNLPPHACLHFFNFLRQ 80

Query: 61  NPS---RKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNE 120
           NPS    +PDL AH+ L  RLY A+KFAEMK V  +IV D NL  ++  ++S I     +
Sbjct: 81  NPSLYSHRPDLQAHVTLICRLYEAQKFAEMKKVFNYIVTDANLRCSIVNLISLIESGNQQ 140

Query: 121 PSIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCV 180
           P  VEKFCD+LFRVY DNR+F+ +L VFDY  + G +IDERSC  FLLALKR  ++E+C 
Sbjct: 141 PKFVEKFCDLLFRVYADNRLFEDALGVFDYMERSGLKIDERSCIAFLLALKRCDHIEMCF 200

Query: 181 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 240
           +F R+MVD+ +EI V S + ++DGLCK+G+V  A+ LMDE+V KG KP++ TYNTLLNG+
Sbjct: 201 KFFRKMVDANVEITVYSISIMIDGLCKRGDVEMARDLMDEMVVKGIKPNLYTYNTLLNGF 260

Query: 241 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 300
           ++ KD  GVNE+LSLM+++ V YNV TYT+L +WY  S K+ E EK+FDEM ++G+E DV
Sbjct: 261 VKRKDAVGVNEMLSLMQRDQVAYNVVTYTLLTDWYGNSGKMVEVEKMFDEMHERGVEADV 320

Query: 301 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 360
           ++YT++I+WNCK GNM+RAF LFD++TER +VPN++ YGA+I+G CK G+++AAEM++ D
Sbjct: 321 HVYTTVISWNCKSGNMRRAFTLFDDLTERGIVPNSHIYGAMIDGVCKVGQVEAAEMLLKD 380

Query: 361 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 420
           MQ +G+DVN VIFNTLM+GYCKKGMIDEAL++  IM++KGFE   F  N IASG C+  R
Sbjct: 381 MQRQGLDVNEVIFNTLMNGYCKKGMIDEALKVMAIMEKKGFEAGPFAYNTIASGLCKVKR 440

Query: 421 REEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNA 480
             EAK+ L TM ERG+A N V+FS LI I CKE N  EA+ LF+ M+ KG  P+  TYN 
Sbjct: 441 YNEAKKWLFTMMERGLALNTVNFSTLIGICCKEGNLEEAKWLFEDMKMKGVKPNSFTYNT 500

Query: 481 FIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLG 540
            I  Y KKG +++AY+L  +M+E G+ PD YTYTSLI GER  G +D A+ LFNEM + G
Sbjct: 501 LIHGYSKKGNIKQAYRLKCKMEEMGMTPDVYTYTSLIHGERVFGKLDEAMILFNEMIEKG 560

Query: 541 LNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 586
              N VTYT +I+GLSK+GR+DEAF+LYDEM   G+ PDD + SSL+A
Sbjct: 561 FIPNAVTYTTLITGLSKEGRSDEAFRLYDEMIASGLTPDDKLCSSLVA 608

BLAST of Cucsa.306820 vs. TrEMBL
Match: A0A165WT71_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_014992 PE=4 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 2.0e-190
Identity = 327/590 (55.42%), Postives = 441/590 (74.75%), Query Frame = 1

Query: 2   SKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQN 61
           S + +A  IAK ++ S  +P +  PSL SN +  ++ LVLS+PN+   SCL FF FL+ N
Sbjct: 22  SDERLAKLIAKTLINSTSKPLQIDPSLTSNLNPNIIHLVLSNPNVKIPSCLHFFRFLKSN 81

Query: 62  P---SRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEP 121
           P   S+KP+L AH+ L  RL++ARKFAE K +L  +V++ NL      IVS +   + EP
Sbjct: 82  PYPFSQKPNLQAHITLVCRLFQARKFAEAKIILNQVVSNENLVCPTSDIVSMMIESYEEP 141

Query: 122 SIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVE 181
            IV K  DMLFRVY DN+ F+  LE+F+Y +  GFEID+RSC  +LLA+KR   ++L  +
Sbjct: 142 KIVHKLFDMLFRVYADNKRFEECLEIFEYMKSNGFEIDDRSCMVYLLAVKRCDQLDLLYD 201

Query: 182 FLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYI 241
           F R+MVDSG++I V S T V+DGLCK GEV +A+ L+DE+V +G +P+  TYNTL++ Y+
Sbjct: 202 FFRRMVDSGVKITVYSMTLVIDGLCKIGEVGKARNLLDEMVDRGIEPNDYTYNTLIDAYM 261

Query: 242 EIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVY 301
           +  D GGV E+L+ MEKN + YNV TYT+LI W SRS ++EEAE+LF+EM ++GI  DV+
Sbjct: 262 KKLDSGGVKEMLNAMEKNGIPYNVATYTLLINWNSRSEEMEEAERLFEEMNQRGILGDVH 321

Query: 302 IYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDM 361
           +YT++I+WNCK GN K+AF LFDEM ER LVPN +TY AL+NG CKAG M+  E+++++M
Sbjct: 322 VYTTMISWNCKLGNTKKAFRLFDEMAERGLVPNCHTYAALVNGLCKAGHMEGVEVLLHEM 381

Query: 362 QSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRR 421
           Q+KG ++NRVIFNTL+DGYCK+GMIDEA R Q+IM+QKGFE D F  + IA G C+ NR 
Sbjct: 382 QTKGYNLNRVIFNTLIDGYCKRGMIDEAWRCQSIMEQKGFEADVFAYSTIAHGLCKLNRH 441

Query: 422 EEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAF 481
           EEAKRLLL+M +RGVAPN+V F+ LIDIYCK+ +F EA+R  + ME KG+ P+VVTYNA 
Sbjct: 442 EEAKRLLLSMVDRGVAPNIVVFTTLIDIYCKQGDFVEAKRTIREMESKGEKPNVVTYNAL 501

Query: 482 IERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGL 541
           I+ YCKK  M+EA+K + EM+ +GL+ D YTYTSL+ GE  SGN+D A  LF+EMP   L
Sbjct: 502 IDGYCKKSMMKEAHKTMREMEVKGLVADVYTYTSLVHGECISGNIDEAARLFSEMPDKRL 561

Query: 542 NRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
             N++TY+V+ISGLSK GR+DEAFKLYD+M + G+ PDD +Y++L+ SLH
Sbjct: 562 VPNIITYSVMISGLSKVGRSDEAFKLYDQMKEAGLTPDDKVYAALVGSLH 611

BLAST of Cucsa.306820 vs. TAIR10
Match: AT2G32630.1 (AT2G32630.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 612.5 bits (1578), Expect = 2.8e-175
Identity = 312/592 (52.70%), Postives = 425/592 (71.79%), Query Frame = 1

Query: 2   SKQAIAANI-AKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 61
           S Q  A  I A L+ KS +   ++ PSLL N +S V +LVLS+P LPT+SC+ FF  LR+
Sbjct: 29  SDQEAARRITACLVEKSTIGKLQSNPSLLFNLNSNVTRLVLSEPTLPTQSCIDFFKLLRE 88

Query: 62  NPSR-KPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSS-IGGEFNEP 121
             S  KPDL A + L  RLY  R+F EM+++L  +VNDG     VE + S+ +  + +E 
Sbjct: 89  FESNLKPDLTAVVTLSHRLYSNRRFNEMRSLLNSVVNDGFYKRPVEELGSAMVDCDISEE 148

Query: 122 SI--VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELC 181
                EKF D++FRVY+DN MF+  L VFDY  KKG  IDERSC  FL+A K+   ++LC
Sbjct: 149 KFEFFEKFFDLVFRVYVDNGMFEEGLRVFDYMVKKGLSIDERSCIVFLVAAKKRRRIDLC 208

Query: 182 VEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNG 241
           +E  R+MVDSG++I V S T VV+GLC++GEV ++K L+ E   KG KP   TYNT++N 
Sbjct: 209 LEIFRRMVDSGVKITVYSLTIVVEGLCRRGEVEKSKKLIKEFSVKGIKPEAYTYNTIINA 268

Query: 242 YIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPD 301
           Y++ +D  GV  +L +M+K+ V YN  TYT+L+E   ++ K+ +AEKLFDEM ++GIE D
Sbjct: 269 YVKQRDFSGVEGVLKVMKKDGVVYNKVTYTLLMELSVKNGKMSDAEKLFDEMRERGIESD 328

Query: 302 VYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVN 361
           V++YTS+I+WNC+ GNMKRAF+LFDE+TE+ L P++YTYGALI+G CK GEM AAE+++N
Sbjct: 329 VHVYTSLISWNCRKGNMKRAFLLFDELTEKGLSPSSYTYGALIDGVCKVGEMGAAEILMN 388

Query: 362 DMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSN 421
           +MQSKGV++ +V+FNTL+DGYC+KGM+DEA  + ++M+QKGF+ D FTCN IAS F R  
Sbjct: 389 EMQSKGVNITQVVFNTLIDGYCRKGMVDEASMIYDVMEQKGFQADVFTCNTIASCFNRLK 448

Query: 422 RREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYN 481
           R +EAK+ L  M E GV  + VS++ LID+YCKE N  EA+RLF  M  KG  P+ +TYN
Sbjct: 449 RYDEAKQWLFRMMEGGVKLSTVSYTNLIDVYCKEGNVEEAKRLFVEMSSKGVQPNAITYN 508

Query: 482 AFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQL 541
             I  YCK+GK++EA KL   M+  G+ PD+YTYTSLI GE  + NVD A+ LF+EM   
Sbjct: 509 VMIYAYCKQGKIKEARKLRANMEANGMDPDSYTYTSLIHGECIADNVDEAMRLFSEMGLK 568

Query: 542 GLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
           GL++N VTYTV+ISGLSK G++DEAF LYDEM ++G   D+ +Y++LI S+H
Sbjct: 569 GLDQNSVTYTVMISGLSKAGKSDEAFGLYDEMKRKGYTIDNKVYTALIGSMH 620

BLAST of Cucsa.306820 vs. TAIR10
Match: AT5G39710.1 (AT5G39710.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 270.8 bits (691), Expect = 2.0e-72
Identity = 150/460 (32.61%), Postives = 259/460 (56.30%), Query Frame = 1

Query: 126 DMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSG-NMELCVEFLRQMV 185
           D++ + Y    + D +L +   A+  GF     S    L A  RS  N+       ++M+
Sbjct: 138 DLVVKSYSRLSLIDKALSIVHLAQAHGFMPGVLSYNAVLDATIRSKRNISFAENVFKEML 197

Query: 186 DSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVG 245
           +S +   V ++  ++ G C  G +  A  L D++  KG  P+V+TYNTL++GY +++ + 
Sbjct: 198 ESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKID 257

Query: 246 GVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSII 305
              ++L  M    ++ N+ +Y ++I    R  +++E   +  EM ++G   D   Y ++I
Sbjct: 258 DGFKLLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLI 317

Query: 306 NWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVD 365
              CK GN  +A V+  EM    L P+  TY +LI+  CKAG M  A   ++ M+ +G+ 
Sbjct: 318 KGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLC 377

Query: 366 VNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRL 425
            N   + TL+DG+ +KG ++EA R+   M   GF     T N + +G C + + E+A  +
Sbjct: 378 PNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAV 437

Query: 426 LLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCK 485
           L  M+E+G++P+VVS+S ++  +C+  +  EA R+ + M +KG  P  +TY++ I+ +C+
Sbjct: 438 LEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCE 497

Query: 486 KGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVT 545
           + + +EA  L  EM   GL PD +TYT+LI+     G++++AL+L NEM + G+  +VVT
Sbjct: 498 QRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVT 557

Query: 546 YTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 585
           Y+V+I+GL+K  R  EA +L  ++  E  VP D  Y +LI
Sbjct: 558 YSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLI 597


HSP 2 Score: 228.8 bits (582), Expect = 8.7e-60
Identity = 133/433 (30.72%), Postives = 225/433 (51.96%), Query Frame = 1

Query: 170 SGNMELCVEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVIT 229
           +GN+++ +    +M   G    V ++  ++DG CK  ++     L+  +  KG +P++I+
Sbjct: 218 AGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLIS 277

Query: 230 YNTLLNGYIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEML 289
           YN ++NG      +  V+ +L+ M +     +  TY  LI+ Y +     +A  +  EML
Sbjct: 278 YNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEML 337

Query: 290 KKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMK 349
           + G+ P V  YTS+I+  CK GNM RA    D+M  R L PN  TY  L++G  + G M 
Sbjct: 338 RHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMN 397

Query: 350 AAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIA 409
            A  ++ +M   G   + V +N L++G+C  G +++A+ +   M++KG   D  + + + 
Sbjct: 398 EAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVL 457

Query: 410 SGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKA 469
           SGFCRS   +EA R+   M E+G+ P+ +++S LI  +C+++   EA  L++ M + G  
Sbjct: 458 SGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLP 517

Query: 470 PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA--- 529
           P   TY A I  YC +G +E+A +L NEM E+G++PD  TY+ LI+G         A   
Sbjct: 518 PDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRL 577

Query: 530 -LELFNEMPQLGLNRNVVTYTVI--------------ISGLSKDGRADEAFKLYDEMNKE 585
            L+LF E     +  +V  +T+I              I G    G   EA ++++ M  +
Sbjct: 578 LLKLFYEE---SVPSDVTYHTLIENCSNIEFKSVVSLIKGFCMKGMMTEADQVFESMLGK 637


HSP 3 Score: 202.6 bits (514), Expect = 6.7e-52
Identity = 123/444 (27.70%), Postives = 216/444 (48.65%), Query Frame = 1

Query: 132 YMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIR 191
           Y   R  D   ++      KG E +  S    +  L R G M+     L +M   G  + 
Sbjct: 250 YCKLRKIDDGFKLLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLD 309

Query: 192 VCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILS 251
             ++  ++ G CK+G   +A  +  E++  G  PSVITY +L++   +  ++    E L 
Sbjct: 310 EVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLD 369

Query: 252 LMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFG 311
            M    +  N  TYT L++ +S+   + EA ++  EM   G  P V  Y ++IN +C  G
Sbjct: 370 QMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTG 429

Query: 312 NMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFN 371
            M+ A  + ++M E+ L P+  +Y  +++G C++ ++  A  +  +M  KG+  + + ++
Sbjct: 430 KMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYS 489

Query: 372 TLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEER 431
           +L+ G+C++    EA  L   M + G   D FT   + + +C     E+A +L   M E+
Sbjct: 490 SLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEK 549

Query: 432 GVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIER---------- 491
           GV P+VV++S+LI+   K+    EA+RL   +  +   PS VTY+  IE           
Sbjct: 550 GVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFKSVV 609

Query: 492 -----YCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQL 551
                +C KG M EA ++   M  +   PD   Y  +I G   +G++ +A  L+ EM + 
Sbjct: 610 SLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEMVKS 669

Query: 552 GLNRNVVTYTVIISGLSKDGRADE 561
           G   + VT   ++  L K+G+ +E
Sbjct: 670 GFLLHTVTVIALVKALHKEGKVNE 693


HSP 4 Score: 168.7 bits (426), Expect = 1.1e-41
Identity = 118/441 (26.76%), Postives = 214/441 (48.53%), Query Frame = 1

Query: 119 SIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVE 178
           S+ E   + L + Y     F  +L +     + G      +    + ++ ++GNM   +E
Sbjct: 307 SLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAME 366

Query: 179 FLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYI 238
           FL QM   G+     ++T +VDG  +KG +  A  ++ E+   GF PSV+TYN L+NG+ 
Sbjct: 367 FLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHC 426

Query: 239 EIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVY 298
               +     +L  M++  +  +V +Y+ ++  + RS  ++EA ++  EM++KGI+PD  
Sbjct: 427 VTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTI 486

Query: 299 IYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDM 358
            Y+S+I   C+    K A  L++EM    L P+ +TY ALIN  C  G+++ A  + N+M
Sbjct: 487 TYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEM 546

Query: 359 QSKGVDVNRVIFNTLMDGYCKKGMIDEALRL-------QNIMQQKGFEIDAFTCNII--- 418
             KGV  + V ++ L++G  K+    EA RL       +++     +      C+ I   
Sbjct: 547 VEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFK 606

Query: 419 -----ASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVM 478
                  GFC      EA ++  +M  +   P+  +++I+I  +C+  +  +A  L+K M
Sbjct: 607 SVVSLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEM 666

Query: 479 EKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNV 538
            K G     VT  A ++   K+GK+ E   +I  +     + +      L++     GN+
Sbjct: 667 VKSGFLLHTVTVIALVKALHKEGKVNELNSVIVHVLRSCELSEAEQAKVLVEINHREGNM 726

Query: 539 DRALELFNEMPQLGLNRNVVT 545
           D  L++  EM + G   N ++
Sbjct: 727 DVVLDVLAEMAKDGFLPNGIS 747

BLAST of Cucsa.306820 vs. TAIR10
Match: AT1G09820.1 (AT1G09820.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 263.1 bits (671), Expect = 4.2e-70
Identity = 169/552 (30.62%), Postives = 292/552 (52.90%), Query Frame = 1

Query: 38  QLVLS--DPNLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIV 97
           QL+ S  DP+L    CLR++ +L +N      L     L   L  A++++++++ L   V
Sbjct: 73  QLISSELDPDL----CLRYYSWLVKNSDISVSLELTFKLLHSLANAKRYSKIRSFLDGFV 132

Query: 98  NDGNLWSNVERIVSSIGGEFNEPSIVEKFC------DMLFRVYMDNRMFDSSLEVFDYAR 157
            +G+     +  V SI   F+  S+ +  C      DML   Y +N  F+   E F  + 
Sbjct: 133 RNGS-----DHQVHSI---FHAISMCDNVCVNSIIADMLVLAYANNSRFELGFEAFKRSG 192

Query: 158 KKGFEIDERSCFEFLLALKRSGNMELCVEFL-RQMVDSGIEIRVCSWTAVVDGLCKKGEV 217
             G+++   SC   ++AL +  N    VE++ ++M+   I+  V ++  V++ LCK G++
Sbjct: 193 YYGYKLSALSCKPLMIALLKE-NRSADVEYVYKEMIRRKIQPNVFTFNVVINALCKTGKM 252

Query: 218 VRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGV---NEILSLMEKNVVDYNVTTY 277
            +A+ +M+++   G  P+V++YNTL++GY ++   G +   + +L  M +N V  N+TT+
Sbjct: 253 NKARDVMEDMKVYGCSPNVVSYNTLIDGYCKLGGNGKMYKADAVLKEMVENDVSPNLTTF 312

Query: 278 TMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTE 337
            +LI+ + +   +  + K+F EML + ++P+V  Y S+IN  C  G +  A  + D+M  
Sbjct: 313 NILIDGFWKDDNLPGSMKVFKEMLDQDVKPNVISYNSLINGLCNGGKISEAISMRDKMVS 372

Query: 338 RRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDE 397
             + PN  TY ALING CK   +K A  M   ++ +G      ++N L+D YCK G ID+
Sbjct: 373 AGVQPNLITYNALINGFCKNDMLKEALDMFGSVKGQGAVPTTRMYNMLIDAYCKLGKIDD 432

Query: 398 ALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILID 457
              L+  M+++G   D  T N + +G CR+   E AK+L   +  +G+ P++V+F IL++
Sbjct: 433 GFALKEEMEREGIVPDVGTYNCLIAGLCRNGNIEAAKKLFDQLTSKGL-PDLVTFHILME 492

Query: 458 IYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQ-ERGLM 517
            YC++    +A  L K M K G  P  +TYN  ++ YCK+G ++ A  +  +M+ ER L 
Sbjct: 493 GYCRKGESRKAAMLLKEMSKMGLKPRHLTYNIVMKGYCKEGNLKAATNMRTQMEKERRLR 552

Query: 518 PDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKL 577
            +  +Y  L+ G    G ++ A  L NEM + GL  N +TY ++                
Sbjct: 553 MNVASYNVLLQGYSQKGKLEDANMLLNEMLEKGLVPNRITYEIV---------------- 594

BLAST of Cucsa.306820 vs. TAIR10
Match: AT2G15630.1 (AT2G15630.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 260.4 bits (664), Expect = 2.7e-69
Identity = 146/462 (31.60%), Postives = 243/462 (52.60%), Query Frame = 1

Query: 126 DMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVD 185
           D+L R     RM D ++E F   ++KGF     +C   L  L R   +E    F   M  
Sbjct: 159 DLLVRCCCQLRMVDEAIECFYLMKEKGFYPKTETCNHILTLLSRLNRIENAWVFYADMYR 218

Query: 186 SGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGG 245
             I+  V ++  +++ LCK+G++ +AK  +  +   G KP+++TYNTL+ G+     + G
Sbjct: 219 MEIKSNVYTFNIMINVLCKEGKLKKAKGFLGIMEVFGIKPTIVTYNTLVQGFSLRGRIEG 278

Query: 246 VNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIIN 305
              I+S M+      ++ TY  ++ W     +   A ++  EM + G+ PD   Y  +I 
Sbjct: 279 ARLIISEMKSKGFQPDMQTYNPILSWMCNEGR---ASEVLREMKEIGLVPDSVSYNILIR 338

Query: 306 WNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDV 365
                G+++ AF   DEM ++ +VP  YTY  LI+G     +++AAE+++ +++ KG+ +
Sbjct: 339 GCSNNGDLEMAFAYRDEMVKQGMVPTFYTYNTLIHGLFMENKIEAAEILIREIREKGIVL 398

Query: 366 NRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLL 425
           + V +N L++GYC+ G   +A  L + M   G +   FT   +    CR N+  EA  L 
Sbjct: 399 DSVTYNILINGYCQHGDAKKAFALHDEMMTDGIQPTQFTYTSLIYVLCRKNKTREADELF 458

Query: 426 LTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKK 485
             +  +G+ P++V  + L+D +C   N   A  L K M+     P  VTYN  +   C +
Sbjct: 459 EKVVGKGMKPDLVMMNTLMDGHCAIGNMDRAFSLLKEMDMMSINPDDVTYNCLMRGLCGE 518

Query: 486 GKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTY 545
           GK EEA +L+ EM+ RG+ PD  +Y +LI G    G+   A  + +EM  LG N  ++TY
Sbjct: 519 GKFEEARELMGEMKRRGIKPDHISYNTLISGYSKKGDTKHAFMVRDEMLSLGFNPTLLTY 578

Query: 546 TVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 588
             ++ GLSK+   + A +L  EM  EGIVP+D  + S+I ++
Sbjct: 579 NALLKGLSKNQEGELAEELLREMKSEGIVPNDSSFCSVIEAM 617

BLAST of Cucsa.306820 vs. TAIR10
Match: AT1G09680.1 (AT1G09680.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 258.8 bits (660), Expect = 7.9e-69
Identity = 150/539 (27.83%), Postives = 283/539 (52.50%), Query Frame = 1

Query: 23  KTTPSLLSNFDSRVMQLVLSDP-NLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRA 82
           K  PSL  +    V+ L+  +P +LP RS   FF F+   P  +  +  + +L   L   
Sbjct: 75  KVLPSLSVH---HVVDLINHNPLSLPQRSIFAFFKFISSQPGFRFTVETYFVLARFLAVH 134

Query: 83  RKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSIVEKFCDMLFRVYMDNRMFDSS 142
             F E +++++ +V+     S     +S +  E     +     D L   Y D      +
Sbjct: 135 EMFTEAQSLIELVVSRKGKNSASSVFISLV--EMRVTPMCGFLVDALMITYTDLGFIPDA 194

Query: 143 LEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIRVCSWTAVVDG 202
           ++ F  +RK  F++  R C   L  + +         F  +++D+G  + V  +  +++ 
Sbjct: 195 IQCFRLSRKHRFDVPIRGCGNLLDRMMKLNPTGTIWGFYMEILDAGFPLNVYVFNILMNK 254

Query: 203 LCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILSLMEKNVVDYN 262
            CK+G +  A+ + DE+  +  +P+V+++NTL+NGY ++ ++     +   MEK+    +
Sbjct: 255 FCKEGNISDAQKVFDEITKRSLQPTVVSFNTLINGYCKVGNLDEGFRLKHQMEKSRTRPD 314

Query: 263 VTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFD 322
           V TY+ LI    + +K++ A  LFDEM K+G+ P+  I+T++I+ + + G +      + 
Sbjct: 315 VFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFTTLIHGHSRNGEIDLMKESYQ 374

Query: 323 EMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKG 382
           +M  + L P+   Y  L+NG CK G++ AA  +V+ M  +G+  +++ + TL+DG+C+ G
Sbjct: 375 KMLSKGLQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRRGLRPDKITYTTLIDGFCRGG 434

Query: 383 MIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFS 442
            ++ AL ++  M Q G E+D    + +  G C+  R  +A+R L  M   G+ P+ V+++
Sbjct: 435 DVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDAERALREMLRAGIKPDDVTYT 494

Query: 443 ILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQER 502
           +++D +CK+ +     +L K M+  G  PSVVTYN  +   CK G+M+ A  L++ M   
Sbjct: 495 MMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVTYNVLLNGLCKLGQMKNADMLLDAMLNI 554

Query: 503 GLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGL---SKDGR 558
           G++PD  TY +L++G     N  +    + + P++G+  ++ +Y  I++ L   SKD R
Sbjct: 555 GVVPDDITYNTLLEGHHRHANSSKR---YIQKPEIGIVADLASYKSIVNELDRASKDHR 605


HSP 2 Score: 152.9 bits (385), Expect = 6.1e-37
Identity = 87/303 (28.71%), Postives = 152/303 (50.17%), Query Frame = 1

Query: 287 EMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAG 346
           E+L  G   +VY++  ++N  CK GN+  A  +FDE+T+R L P   ++  LING CK G
Sbjct: 230 EILDAGFPLNVYVFNILMNKFCKEGNISDAQKVFDEITKRSLQPTVVSFNTLINGYCKVG 289

Query: 347 EMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCN 406
            +     + + M+      +   ++ L++  CK+  +D A  L + M ++G   +     
Sbjct: 290 NLDEGFRLKHQMEKSRTRPDVFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFT 349

Query: 407 IIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKK 466
            +  G  R+   +  K     M  +G+ P++V ++ L++ +CK  +   AR +   M ++
Sbjct: 350 TLIHGHSRNGEIDLMKESYQKMLSKGLQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRR 409

Query: 467 GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 526
           G  P  +TY   I+ +C+ G +E A ++  EM + G+  D   +++L+ G    G V  A
Sbjct: 410 GLRPDKITYTTLIDGFCRGGDVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDA 469

Query: 527 LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIAS 586
                EM + G+  + VTYT+++    K G A   FKL  EM  +G VP    Y+ L+  
Sbjct: 470 ERALREMLRAGIKPDDVTYTMMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVTYNVLLNG 529

Query: 587 LHK 590
           L K
Sbjct: 530 LCK 532


HSP 3 Score: 121.3 bits (303), Expect = 2.0e-27
Identity = 65/241 (26.97%), Postives = 131/241 (54.36%), Query Frame = 1

Query: 344 KAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAF 403
           + G+  A+ + ++ ++ +   +   + + LM  Y   G I +A++   + ++  F++   
Sbjct: 147 RKGKNSASSVFISLVEMRVTPMCGFLVDALMITYTDLGFIPDAIQCFRLSRKHRFDVPIR 206

Query: 404 TCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVM 463
            C  +     + N         + + + G   NV  F+IL++ +CKE N ++A+++F  +
Sbjct: 207 GCGNLLDRMMKLNPTGTIWGFYMEILDAGFPLNVYVFNILMNKFCKEGNISDAQKVFDEI 266

Query: 464 EKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNV 523
            K+   P+VV++N  I  YCK G ++E ++L ++M++    PD +TY++LI+       +
Sbjct: 267 TKRSLQPTVVSFNTLINGYCKVGNLDEGFRLKHQMEKSRTRPDVFTYSALINALCKENKM 326

Query: 524 DRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSL 583
           D A  LF+EM + GL  N V +T +I G S++G  D   + Y +M  +G+ PD  +Y++L
Sbjct: 327 DGAHGLFDEMCKRGLIPNDVIFTTLIHGHSRNGEIDLMKESYQKMLSKGLQPDIVLYNTL 386

Query: 584 I 585
           +
Sbjct: 387 V 387

BLAST of Cucsa.306820 vs. NCBI nr
Match: gi|449440401|ref|XP_004137973.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Cucumis sativus])

HSP 1 Score: 1172.9 bits (3033), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ
Sbjct: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60

Query: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120
           NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI
Sbjct: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120

Query: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180
           VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL
Sbjct: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180

Query: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240
           RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI
Sbjct: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240

Query: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300
           KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY
Sbjct: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300

Query: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360
           TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS
Sbjct: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360

Query: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420
           KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE
Sbjct: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420

Query: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480
           AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE
Sbjct: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480

Query: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR 540
           RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR
Sbjct: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR 540

Query: 541 NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK
Sbjct: 541 NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 589

BLAST of Cucsa.306820 vs. NCBI nr
Match: gi|659084068|ref|XP_008442691.1| (PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At2g32630-like [Cucumis melo])

HSP 1 Score: 1046.2 bits (2704), Expect = 2.2e-302
Identity = 520/589 (88.29%), Postives = 555/589 (94.23%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           MS QAIA NIAKLILKSGLQPFKTTPSLLS  DSRV Q +LSDPN+PT+SCLRFF+FLR+
Sbjct: 1   MSNQAIATNIAKLILKSGLQPFKTTPSLLSKLDSRVTQSILSDPNVPTKSCLRFFNFLRR 60

Query: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120
           NPS KPDLPAHLIL  RLYRARKFAEMKNVLKFIVN GNLWSNVERIVSSIGGEFNEP  
Sbjct: 61  NPSCKPDLPAHLILVCRLYRARKFAEMKNVLKFIVNGGNLWSNVERIVSSIGGEFNEPKF 120

Query: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180
           VE FCDMLFRVYMD RMFDSSLEVFDYARK GFEI+ERSCFEFLLALKRSGNMELCVEFL
Sbjct: 121 VENFCDMLFRVYMDKRMFDSSLEVFDYARKNGFEIEERSCFEFLLALKRSGNMELCVEFL 180

Query: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240
           RQ+VDSGIEIRV SWTAVVDGLCKKGEVVRAKAL+DELVCKGFKP+VITYNTLLNGYIEI
Sbjct: 181 RQIVDSGIEIRVSSWTAVVDGLCKKGEVVRAKALVDELVCKGFKPNVITYNTLLNGYIEI 240

Query: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300
           KD GGVNEILSLMEK+VVDYNV TYTMLIEWYSR SKIEE+EKLFDEMLKKGIEPDVY+Y
Sbjct: 241 KDEGGVNEILSLMEKDVVDYNVATYTMLIEWYSRISKIEESEKLFDEMLKKGIEPDVYVY 300

Query: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360
           TS+INWNCKFGNMKRAFVLFDEMTERRLVPNA+TYGAL+NGACKAG+M+AAEMMVNDMQS
Sbjct: 301 TSLINWNCKFGNMKRAFVLFDEMTERRLVPNAFTYGALVNGACKAGQMEAAEMMVNDMQS 360

Query: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420
           KGVDVN+VIFNTL+DGYC KGMIDEALRLQNIMQQKGFEID FTCNIIASGFCR +RREE
Sbjct: 361 KGVDVNQVIFNTLIDGYCXKGMIDEALRLQNIMQQKGFEIDVFTCNIIASGFCRLDRREE 420

Query: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480
           A+RLLLTMEERGVAP+VVSFSILIDIY KE+NFAEA+RL K MEKKG+ PSVVTYN FI+
Sbjct: 421 ARRLLLTMEERGVAPDVVSFSILIDIYGKEENFAEAKRLLKEMEKKGEVPSVVTYNTFIQ 480

Query: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR 540
           RYCKKGKMEEAYKLI+EM+ERGLMPDTYTYTSL++ E ASGNVDRALELFNEMPQ GLNR
Sbjct: 481 RYCKKGKMEEAYKLIDEMKERGLMPDTYTYTSLMNWECASGNVDRALELFNEMPQRGLNR 540

Query: 541 NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           N VTYTV+ISGLSK GRADEAFKLYDEMN +GI+PDD I+SSLIASLH+
Sbjct: 541 NEVTYTVMISGLSKAGRADEAFKLYDEMNTQGIIPDDRIFSSLIASLHE 589

BLAST of Cucsa.306820 vs. NCBI nr
Match: gi|700203836|gb|KGN58969.1| (hypothetical protein Csa_3G740130 [Cucumis sativus])

HSP 1 Score: 915.6 bits (2365), Expect = 4.4e-263
Identity = 469/528 (88.83%), Postives = 481/528 (91.10%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ
Sbjct: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60

Query: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120
           NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI
Sbjct: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120

Query: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180
           VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL
Sbjct: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180

Query: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240
           RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI
Sbjct: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240

Query: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300
           KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY
Sbjct: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300

Query: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360
           TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS
Sbjct: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360

Query: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420
           KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE
Sbjct: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420

Query: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480
           AKRLLLTMEERGVAPNVVSF    +      N   A  LF  M + G   +VVTY   I 
Sbjct: 421 AKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTVIIS 480

Query: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALE 529
              K G+ +EA+KL +EM + G++PD   Y+SLI      G +   LE
Sbjct: 481 GLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHKVGPLVSGLE 524

BLAST of Cucsa.306820 vs. NCBI nr
Match: gi|700203836|gb|KGN58969.1| (hypothetical protein Csa_3G740130 [Cucumis sativus])

HSP 1 Score: 264.2 bits (674), Expect = 5.3e-67
Identity = 142/342 (41.52%), Postives = 215/342 (62.87%), Query Frame = 1

Query: 248 EILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWN 307
           E L  M  + ++  V ++T +++   +  ++  A+ L DE++ KG +P V  Y +++N  
Sbjct: 178 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 237

Query: 308 CKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNR 367
            +  ++     +   M +  +  N  TY  LI    ++ +++ AE + ++M  KG++ + 
Sbjct: 238 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 297

Query: 368 VIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLT 427
            I+ ++++  CK G +  A  L + M ++    +A+T   + +G C++   + A+ ++  
Sbjct: 298 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 357

Query: 428 MEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGK 487
           M+ +GV  N V F+ L+D YCK+    EA RL  +M++KG      T N     +C+  +
Sbjct: 358 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 417

Query: 488 MEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTV 547
            EEA +L+  M+ERG+ P+  ++    +GERASGNVDRALELFNEMPQLGLNRNVVTYTV
Sbjct: 418 REEAKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTV 477

Query: 548 IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK
Sbjct: 478 IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 515


HSP 2 Score: 148.7 bits (374), Expect = 3.3e-32
Identity = 85/310 (27.42%), Postives = 160/310 (51.61%), Query Frame = 1

Query: 267 MLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTER 326
           ML   Y  +   + + ++FD   KKG E D       +    + GNM+       +M + 
Sbjct: 127 MLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDS 186

Query: 327 RLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEA 386
            +     ++ A+++G CK GE+  A+ +++++  KG   + + +NTL++GY +   +   
Sbjct: 187 GIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGV 246

Query: 387 LRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDI 446
             + ++M++   + +  T  ++   + RS++ EEA++L   M ++G+ P+V  ++ +I+ 
Sbjct: 247 NEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINW 306

Query: 447 YCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPD 506
            CK  N   A  LF  M ++   P+  TY A I   CK G+M+ A  ++N+MQ +G+  +
Sbjct: 307 NCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVN 366

Query: 507 TYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYD 566
              + +L+DG    G +D AL L N M Q G   +  T  +I SG  +  R +EA +L  
Sbjct: 367 RVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLL 426

Query: 567 EMNKEGIVPD 577
            M + G+ P+
Sbjct: 427 TMEERGVAPN 436


HSP 3 Score: 719.5 bits (1856), Expect = 4.6e-204
Identity = 359/591 (60.74%), Postives = 452/591 (76.48%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           +S Q IA  I+K ++ SGLQP K+TPSLL N +  +  LVLS+P +P  SCL  F+FL+ 
Sbjct: 21  ISNQEIAQKISKTLINSGLQPLKSTPSLLFNLNPHITNLVLSNPLVPPLSCLSLFNFLQT 80

Query: 61  NPS---RKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNE 120
           NPS    KP+L AH+ L  RLY+A+KFA+MK VL  IV++  L   V  IVS    E   
Sbjct: 81  NPSLTSHKPNLQAHVTLICRLYQAKKFAQMKIVLNGIVSNDILRCPVSEIVSLAEDERKG 140

Query: 121 PSIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCV 180
              VE  CDMLFRVY DN+MF+ ++ VFDYA KKGFEI+ERSCF  LLALK+ G ++LC+
Sbjct: 141 VKFVETLCDMLFRVYADNKMFEEAVGVFDYAEKKGFEIEERSCFVLLLALKKCGQVDLCL 200

Query: 181 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 240
            F  QMV+ G++I V S T V++ LCK+GEV +AKALM E+  +  KP+V+TYNTLL  Y
Sbjct: 201 RFFDQMVEKGVQITVHSLTLVMNELCKRGEVKKAKALMGEMAGRQIKPNVVTYNTLLKAY 260

Query: 241 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 300
           IE KD  GVNE LSLMEK+ V YNV T T+LI+W+    KIE+AEK+F+EM ++GI+PDV
Sbjct: 261 IERKDYEGVNEALSLMEKDGVGYNVATCTLLIDWFGSCEKIEDAEKVFEEMHERGIDPDV 320

Query: 301 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 360
           YIYTSIINWN + GNMKRA  LFDE+T+R LVPN +TYGALINGA K G+MK A+++VN+
Sbjct: 321 YIYTSIINWNGRVGNMKRALFLFDELTQRGLVPNVHTYGALINGAYKGGQMKMADILVNE 380

Query: 361 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 420
           MQSKG+DVN+V+FNTL+DGYC+KGM+DE LRLQ+ M+ KGF+ D FT + IASG C+ NR
Sbjct: 381 MQSKGIDVNQVVFNTLIDGYCRKGMMDEVLRLQDFMEGKGFQTDVFTYSTIASGLCKLNR 440

Query: 421 REEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNA 480
            EEAK LL TMEERGVAPN+V F+ LIDI+CKE NF EA+++ + ME+KG+ P+ VTYNA
Sbjct: 441 NEEAKSLLFTMEERGVAPNLVCFTTLIDIFCKEGNFVEAKQVIQEMERKGERPNTVTYNA 500

Query: 481 FIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLG 540
            I+ + KKGKM+EA+KL  EM+++GLMPDTYTY+SLI GE     VD AL+LF+EM Q  
Sbjct: 501 LIDGHIKKGKMKEAHKLKKEMEDKGLMPDTYTYSSLIHGECIDAKVDEALKLFHEMYQRD 560

Query: 541 LNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
           L RNVVTYT +ISGLSKDGR DEAFKLYDEM +EG+ PDD +Y SL+ SLH
Sbjct: 561 LTRNVVTYTAMISGLSKDGRTDEAFKLYDEMKREGLTPDDRVYHSLVGSLH 611

BLAST of Cucsa.306820 vs. NCBI nr
Match: gi|694403797|ref|XP_009376813.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Pyrus x bretschneideri])

HSP 1 Score: 713.4 bits (1840), Expect = 3.3e-202
Identity = 352/589 (59.76%), Postives = 446/589 (75.72%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTT-PSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLR 60
           +S Q  A  IAK ++KSGLQP K+T PSLLSN +  V  LVLS+P +P  SCL  F+FL+
Sbjct: 20  ISNQETAEKIAKTLIKSGLQPLKSTSPSLLSNLNPHVTNLVLSNPLVPPHSCLTLFNFLQ 79

Query: 61  QNPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPS 120
            +PS KP+L AH IL  RLY AR+FA+MK VL  IV + NL   V +I   I  E +   
Sbjct: 80  TDPSHKPNLQAHAILIRRLYNARRFAQMKIVLNGIVTNDNLRCPVSKITCLIEDEPDGVK 139

Query: 121 IVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEF 180
            V   CDMLFRVY DN+MF+ ++ VF+Y  K G EI+ERSCF  LLALK+ G ++LC+ F
Sbjct: 140 FVGTLCDMLFRVYADNKMFEEAIGVFEYVGKNGLEIEERSCFVLLLALKKCGRVDLCLRF 199

Query: 181 LRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIE 240
             QMV+ G+ I V   T V++ LCKKGEV +AKALM E   +G KP+V T+NTLL  YIE
Sbjct: 200 FDQMVEKGVRITVYPLTLVMNELCKKGEVEKAKALMRETADRGTKPNVFTFNTLLKAYIE 259

Query: 241 IKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYI 300
            KD  GVNE+L LMEK+ V YNV T T+LI+W+  S KIE+AEK+F+EM ++GI+PDVY+
Sbjct: 260 RKDFEGVNEVLRLMEKDGVGYNVATCTLLIDWFGSSEKIEDAEKVFEEMRERGIQPDVYV 319

Query: 301 YTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQ 360
           YTSIINWNC+ GNMKRA  LFDE T+R  VPN +TYGALINGACKAG+++ A+++VN+MQ
Sbjct: 320 YTSIINWNCRAGNMKRALFLFDECTQRGFVPNDHTYGALINGACKAGQLEMADILVNEMQ 379

Query: 361 SKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRRE 420
            KG+DVN+V+FNTLMDGYC+ GM+D+ALRLQ++M+ KGF++D FT + IASG C+ NR E
Sbjct: 380 GKGIDVNQVVFNTLMDGYCRSGMMDQALRLQDVMENKGFQLDVFTYSTIASGLCKLNRNE 439

Query: 421 EAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFI 480
           EAK LL TM ERGVAPNVV F+ LIDI+CKE NF EA R+F+ ME+KG+ P+ VTYNA I
Sbjct: 440 EAKSLLFTMAERGVAPNVVCFTTLIDIFCKEGNFVEANRVFQDMERKGERPNTVTYNALI 499

Query: 481 ERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLN 540
           + Y KKGKM+EA+KL  EM+++GLMPD YTY+SLI+G+   G VD AL+LF+EM +  + 
Sbjct: 500 DGYIKKGKMKEAHKLKEEMEDKGLMPDMYTYSSLINGKCIDGKVDEALKLFDEMSRRDIP 559

Query: 541 RNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
            NVVTYT +ISGLSKDGR +EAFKLYDEM   G+ PDD +Y SL+ SLH
Sbjct: 560 HNVVTYTAMISGLSKDGRTEEAFKLYDEMKNAGLTPDDRVYHSLVGSLH 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP180_ARATH5.0e-17452.70Pentatricopeptide repeat-containing protein At2g32630 OS=Arabidopsis thaliana GN... [more]
PP407_ARATH3.6e-7132.61Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana GN... [more]
PPR27_ARATH7.4e-6930.62Pentatricopeptide repeat-containing protein At1g09820 OS=Arabidopsis thaliana GN... [more]
PP152_ARATH4.8e-6831.60Pentatricopeptide repeat-containing protein At2g15630, mitochondrial OS=Arabidop... [more]
PPR26_ARATH1.4e-6727.83Putative pentatricopeptide repeat-containing protein At1g09680 OS=Arabidopsis th... [more]
Match NameE-valueIdentityDescription
A0A0A0LDI0_CUCSA3.1e-26388.83Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1[more]
A0A0A0LDI0_CUCSA3.7e-6741.52Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1[more]
V4S3K8_9ROSI2.7e-19556.63Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011284mg PE=4 SV=1[more]
A0A067DGQ8_CITSI6.1e-19556.63Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g047794mg PE=4 SV=1[more]
A0A165WT71_DAUCA2.0e-19055.42Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_014992 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G32630.12.8e-17552.70 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT5G39710.12.0e-7232.61 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT1G09820.14.2e-7030.62 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT2G15630.12.7e-6931.60 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT1G09680.17.9e-6927.83 Pentatricopeptide repeat (PPR) superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449440401|ref|XP_004137973.1|0.0e+00100.00PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Cucumis sativu... [more]
gi|659084068|ref|XP_008442691.1|2.2e-30288.29PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At2g... [more]
gi|700203836|gb|KGN58969.1|4.4e-26388.83hypothetical protein Csa_3G740130 [Cucumis sativus][more]
gi|700203836|gb|KGN58969.1|5.3e-6741.52hypothetical protein Csa_3G740130 [Cucumis sativus][more]
gi|694403797|ref|XP_009376813.1|3.3e-20259.76PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Pyrus x bretsc... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom_sf
IPR002885Pentatricopeptide_repeat
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.306820.1Cucsa.306820.1mRNA
Cucsa.306820.2Cucsa.306820.2mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 79..126
score: 5.2E-15coord: 8..54
score: 3.3
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 81..114
score: 5.8E-11coord: 46..79
score: 1.1E-4coord: 11..45
score: 6.4
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 9..43
score: 14.063coord: 44..78
score: 10.83coord: 79..113
score: 13
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 1..125
score: 4.4

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.306820Cucumber (Chinese Long) v3cgycucB475
Cucsa.306820Cucumber (Chinese Long) v3cgycucB476
Cucsa.306820Cucumber (Chinese Long) v3cgycucB477
Cucsa.306820Watermelon (97103) v2cgywmbB530
Cucsa.306820Watermelon (97103) v2cgywmbB532
Cucsa.306820Wax gourdcgywgoB612
Cucsa.306820Wax gourdcgywgoB614
Cucsa.306820Cucumber (Gy14) v1cgycgyB074
Cucsa.306820Cucumber (Gy14) v1cgycgyB143
Cucsa.306820Cucumber (Gy14) v1cgycgyB144
Cucsa.306820Cucurbita maxima (Rimu)cgycmaB0844
Cucsa.306820Cucurbita maxima (Rimu)cgycmaB0846
Cucsa.306820Cucurbita maxima (Rimu)cgycmaB0847
Cucsa.306820Cucurbita moschata (Rifu)cgycmoB0838
Cucsa.306820Cucurbita moschata (Rifu)cgycmoB0839
Cucsa.306820Cucurbita moschata (Rifu)cgycmoB0841
Cucsa.306820Cucurbita moschata (Rifu)cgycmoB0844
Cucsa.306820Cucurbita moschata (Rifu)cgycmoB0845
Cucsa.306820Wild cucumber (PI 183967)cgycpiB462
Cucsa.306820Wild cucumber (PI 183967)cgycpiB463
Cucsa.306820Cucumber (Chinese Long) v2cgycuB439
Cucsa.306820Cucumber (Chinese Long) v2cgycuB440
Cucsa.306820Melon (DHL92) v3.5.1cgymeB503
Cucsa.306820Melon (DHL92) v3.5.1cgymeB507
Cucsa.306820Watermelon (Charleston Gray)cgywcgB528
Cucsa.306820Watermelon (97103) v1cgywmB554
Cucsa.306820Cucurbita pepo (Zucchini)cgycpeB0798
Cucsa.306820Cucurbita pepo (Zucchini)cgycpeB0799
Cucsa.306820Cucurbita pepo (Zucchini)cgycpeB0800
Cucsa.306820Cucurbita pepo (Zucchini)cgycpeB0802
Cucsa.306820Bottle gourd (USVL1VR-Ls)cgylsiB494
Cucsa.306820Melon (DHL92) v3.6.1cgymedB502
Cucsa.306820Melon (DHL92) v3.6.1cgymedB503
Cucsa.306820Melon (DHL92) v3.6.1cgymedB507
Cucsa.306820Silver-seed gourdcarcgyB0196