Cucsa.306820.2 (mRNA) Cucumber (Gy14) v1

NameCucsa.306820.2
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPentatricopeptide repeat-containing protein family
Locationscaffold02977 : 235870 .. 237775 (+)
Sequence length1311
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGAAGGGGAAGGCACCTAGTGTTGTTACGTACAATGCTTTCATTGAAAGATATTGCAAGAAGGGAAAGATGGAGGAGGCATACAAACTGATAAATGAGATGCAAGAAAGGGGGTTAATGCCGGACACATATACATATACATCACTTATAGATGGGGAACGTGCTAGTGGGAATGTGGATAGAGCGCTTGAACTATTCAATGAAATGCCACAACTAGGACTAAATCGAAATGTAGTAACTTACACGGTAATAATCTCTGGGTTGTCCAAGGATGGTAGAGCTGATGAAGCTTTTAAATTATATGATGAAATGAACAAAGAAGGCATTGTACCTGATGACGGAATATATTCTTCCTTGATAGCGAGCCTTCATAAGGTAGGACCTTTAGTTTCGGGCTTAGAAAATGTCGTCGACCGCATTGAAACTTGACCAGACTGGGTGTAACAAGCTACAATTTTTTAAAGTTTGAATGTCTTTGAACGAAGATTTGCGGACGAAAAGTTGTGACCCTATTTTAAGGGAGTGCAAAAACTTCCAAGGATAGCACGTAGGAAGTTTGATAAAGCCCATAATGCATTATATTTGCCTCTTCAAGGGTCCAATATGGCGATTTCTCTCGGGAAGACCGGCGTTTATGAGCTATTCAAATGCATTGAGACAATCCCAAGGCATTCAAAGACGCCCTACAGAAAACCGAGGTGGGGCACTATACCCATTTCATGTATAGTGTGTAGGGGTGGGCGAAAGAAaCCATTCATTCAAACAAAGACCACCCTACTACCAAAACGACTCGGACTGATGAACAAAACAAGATCATTTTGTTTCATTTTGTTTCATTTTTAGTACAATTGAGTGGAAAGTGATGTCAATTTGTTCAATACTATCAGCTAGGGAAAACGTCAACATGGGAATGCCATATTAGTCTGCTGTAAAACTGTAATTATGGGAAACGGTTTCAGATGATTATAAGACAAATCCTTTGTTTCCATATCTGATATCAAGAAGAAAACTTGATACTTTCAGAGAAATACATTTGCATTTCTGTCAAAAGAATCATTACAATCTGTTTCTCCCTCAAGATTCCAACCAATTAGGCCAAACCGTATACAACTACAAAAATAAGCAAATACAGACAACCCTGCTTAAGCATTATGGGGAACAACAGCAGAAAAAGAAGGAAAAGATCAATGTGCTTTGTCTTTATCATAAAAGACTTCAAGGCATGTGAATGTAAATCCTAACAAAGGATCGCACTTGAAAAATAGATGTGTAGCAATAACAGGACCAAAAAaGTTAGGAGTCTGAGGCAGTAGGGGGgCTTATCAATGCTTGTTTCCAAACTTCCACCATCTTTTCTTCTTTCTCAACTCACTGGGTGGGGAATCTCCAGTTGATGGCATACCTGAATGTATATATCAAAACAACAAAAATGGATCAAGCTAGAACAAAGTAAAAGCTCAAACATACAAAGTTATGAATATGTCCCTTATCGCTGAGGCCTTGAAGGTGGTAAACATATTATATATCAAATATTCCCAAAAAGTTCTGAAAGCATCAATGGTTGACGTCCTAAATCGGCCATCAAAGTCTTGTCGGCACGTTTGGGAAaAAAAaTGAAAGTAACTTCTCACAGAAGACACTTTATTTCTTTCTATTTAACTAAATATACATTATGTATTCTCGTAATGAAGAATAACCTAGAAGGGTCAAGGGTTACCTAAAACTTAGTTTTGGCTAAAGCAAACAAGTCTCTTTTCTTTTCCTTCTTTTTCTCCCATTTTGTAGGCTTAGCTCTTGGCTATTTTATGAGACTACCAATTCAAGCAACGGAAACACAATAGAGAACAGAGAACAGTATTTACCTCCCTGAGGATGAATTGAGTTGTAATGCACCTCTGCC

mRNA sequence

ATGGAGAAGAAGGGGAAGGCACCTAGTGTTGTTACGTACAATGCTTTCATTGAAAGATATTGCAAGAAGGGAAAGATGGAGGAGGCATACAAACTGATAAATGAGATGCAAGAAAGGGGGTTAATGCCGGACACATATACATATACATCACTTATAGATGGGGAACGTGCTAGTGGGAATGTGGATAGAGCGCTTGAACTATTCAATGAAATGCCACAACTAGGACTAAATCGAAATGTAGTAACTTACACGGTAATAATCTCTGGGTTGTCCAAGGATGGTAGAGCTGATGAAGCTTTTAAATTATATGATGAAATGAACAAAGAAGGCATTGTACCTGATGACGGAATATATTCTTCCTTGATAGCGAGCCTTCATAAGACAAATCCTTTGTTTCCATATCTGATATCAAGAAGAAAACTTGATACTTTCAGAGAAATACATTTGCATTTCTGTCAAAAGAATCATTACAATCTGTTTCTCCCTCAAGATTCCAACCAATTAGGCCAAACCGTATACAACTACAAAAATAAGCAAATACAGACAACCCTGCTTAAGCATTATGGGGAACAACAGCAGAAAAAGAAGGAAAAGATCAATGTGCTTTGTCTTTATCATAAAAGACTTCAAGGCATGTGAATGTAAATCCTAACAAAGGATCGCACTTGAAAAATAGATGTGTAGCAATAACAGGACCAAAAAAGTTAGGAGTCTGAGGCAGTAGGGGGGCTTATCAATGCTTGTTTCCAAACTTCCACCATCTTTTCTTCTTTCTCAACTCACTGGGTGGGGAATCTCCAGTTGATGGCATACCTGAATGTATATATCAAAACAACAAAAATGGATCAAGCTAGAACAAAGTAAAAGCTCAAACATACAAAGTTATGAATATGTCCCTTATCGCTGAGGCCTTGAAGGTGGTAAACATATTATATATCAAATATTCCCAAAAAGTTCTGAAAGCATCAATGGTTGACGTCCTAAATCGGCCATCAAAGTCTTGTCGGCACGTTTGGGAAAAAAAATGAAAGTAACTTCTCACAGAAGACACTTTATTTCTTTCTATTTAACTAAATATACATTATGTATTCTCGTAATGAAGAATAACCTAGAAGGGTCAAGGGTTACCTAAAACTTAGTTTTGGCTAAAGCAAACAAGTCTCTTTTCTTTTCCTTCTTTTTCTCCCATTTTGTAGGCTTAGCTCTTGGCTATTTTATGAGACTACCAATTCAAGCAACGGAAACACAATAGAGAACAGAGAACAGTATTTACCTCCCTGAGGATGAATTGAGTTGTAATGCACCTCTGCC

Coding sequence (CDS)

ATGGAGAAGAAGGGGAAGGCACCTAGTGTTGTTACGTACAATGCTTTCATTGAAAGATATTGCAAGAAGGGAAAGATGGAGGAGGCATACAAACTGATAAATGAGATGCAAGAAAGGGGGTTAATGCCGGACACATATACATATACATCACTTATAGATGGGGAACGTGCTAGTGGGAATGTGGATAGAGCGCTTGAACTATTCAATGAAATGCCACAACTAGGACTAAATCGAAATGTAGTAACTTACACGGTAATAATCTCTGGGTTGTCCAAGGATGGTAGAGCTGATGAAGCTTTTAAATTATATGATGAAATGAACAAAGAAGGCATTGTACCTGATGACGGAATATATTCTTCCTTGATAGCGAGCCTTCATAAGACAAATCCTTTGTTTCCATATCTGATATCAAGAAGAAAACTTGATACTTTCAGAGAAATACATTTGCATTTCTGTCAAAAGAATCATTACAATCTGTTTCTCCCTCAAGATTCCAACCAATTAGGCCAAACCGTATACAACTACAAAAATAAGCAAATACAGACAACCCTGCTTAAGCATTATGGGGAACAACAGCAGAAAAAGAAGGAAAAGATCAATGTGCTTTGTCTTTATCATAAAAGACTTCAAGGCATGTGA

Protein sequence

MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHKTNPLFPYLISRRKLDTFREIHLHFCQKNHYNLFLPQDSNQLGQTVYNYKNKQIQTTLLKHYGEQQQKKKEKINVLCLYHKRLQGM*
BLAST of Cucsa.306820.2 vs. Swiss-Prot
Match: PP180_ARATH (Pentatricopeptide repeat-containing protein At2g32630 OS=Arabidopsis thaliana GN=At2g32630 PE=3 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 4.0e-33
Identity = 69/126 (54.76%), Postives = 92/126 (73.02%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  KG  P+ +TYN  I  YCK+GK++EA KL   M+  G+ PD+YTYTSLI GE  + N
Sbjct: 495 MSSKGVQPNAITYNVMIYAYCKQGKIKEARKLRANMEANGMDPDSYTYTSLIHGECIADN 554

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD A+ LF+EM   GL++N VTYTV+ISGLSK G++DEAF LYDEM ++G   D+ +Y++
Sbjct: 555 VDEAMRLFSEMGLKGLDQNSVTYTVMISGLSKAGKSDEAFGLYDEMKRKGYTIDNKVYTA 614

Query: 121 LIASLH 127
           LI S+H
Sbjct: 615 LIGSMH 620


HSP 2 Score: 85.5 bits (210), Expect = 7.7e-16
Identity = 47/122 (38.52%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+K G   + VTY   +E   K GKM +A KL +EM+ERG+  D + YTSLI      GN
Sbjct: 285 MKKDGVVYNKVTYTLLMELSVKNGKMSDAEKLFDEMRERGIESDVHVYTSLISWNCRKGN 344

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + RA  LF+E+ + GL+ +  TY  +I G+ K G    A  L +EM  +G+     ++++
Sbjct: 345 MKRAFLLFDELTEKGLSPSSYTYGALIDGVCKVGEMGAAEILMNEMQSKGVNITQVVFNT 404

Query: 121 LI 123
           LI
Sbjct: 405 LI 406


HSP 3 Score: 84.3 bits (207), Expect = 1.7e-15
Identity = 43/114 (37.72%), Postives = 62/114 (54.39%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++G    V  Y + I   C+KG M+ A+ L +E+ E+GL P +YTY +LIDG    G 
Sbjct: 320 MRERGIESDVHVYTSLISWNCRKGNMKRAFLLFDELTEKGLSPSSYTYGALIDGVCKVGE 379

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPD 115
           +  A  L NEM   G+N   V +  +I G  + G  DEA  +YD M ++G   D
Sbjct: 380 MGAAEILMNEMQSKGVNITQVVFNTLIDGYCRKGMVDEASMIYDVMEQKGFQAD 433


HSP 4 Score: 77.4 bits (189), Expect = 2.1e-13
Identity = 46/122 (37.70%), Postives = 63/122 (51.64%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME+KG    V T N     + +  + +EA + +  M E G+   T +YT+LID     GN
Sbjct: 425 MEQKGFQADVFTCNTIASCFNRLKRYDEAKQWLFRMMEGGVKLSTVSYTNLIDVYCKEGN 484

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           V+ A  LF EM   G+  N +TY V+I    K G+  EA KL   M   G+ PD   Y+S
Sbjct: 485 VEEAKRLFVEMSSKGVQPNAITYNVMIYAYCKQGKIKEARKLRANMEANGMDPDSYTYTS 544

Query: 121 LI 123
           LI
Sbjct: 545 LI 546


HSP 5 Score: 74.7 bits (182), Expect = 1.4e-12
Identity = 42/127 (33.07%), Postives = 66/127 (51.97%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G   S V+Y   I+ YCK+G +EEA +L  EM  +G+ P+  TY  +I      G 
Sbjct: 460 MMEGGVKLSTVSYTNLIDVYCKEGNVEEAKRLFVEMSSKGVQPNAITYNVMIYAYCKQGK 519

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A +L   M   G++ +  TYT +I G       DEA +L+ EM  +G+  +   Y+ 
Sbjct: 520 IKEARKLRANMEANGMDPDSYTYTSLIHGECIADNVDEAMRLFSEMGLKGLDQNSVTYTV 579

Query: 121 LIASLHK 128
           +I+ L K
Sbjct: 580 MISGLSK 586


HSP 6 Score: 74.7 bits (182), Expect = 1.4e-12
Identity = 42/126 (33.33%), Postives = 74/126 (58.73%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDG---ERA 60
           M   G   +V +    +E  C++G++E++ KLI E   +G+ P+ YTY ++I+    +R 
Sbjct: 215 MVDSGVKITVYSLTIVVEGLCRRGEVEKSKKLIKEFSVKGIKPEAYTYNTIINAYVKQRD 274

Query: 61  SGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGI 120
              V+  L++   M + G+  N VTYT+++    K+G+  +A KL+DEM + GI  D  +
Sbjct: 275 FSGVEGVLKV---MKKDGVVYNKVTYTLLMELSVKNGKMSDAEKLFDEMRERGIESDVHV 334

Query: 121 YSSLIA 124
           Y+SLI+
Sbjct: 335 YTSLIS 337


HSP 7 Score: 71.2 bits (173), Expect = 1.5e-11
Identity = 39/128 (30.47%), Postives = 66/128 (51.56%), Query Frame = 1

Query: 4   KGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 63
           KG  P   TYN  I  Y K+        ++  M++ G++ +  TYT L++    +G +  
Sbjct: 253 KGIKPEAYTYNTIINAYVKQRDFSGVEGVLKVMKKDGVVYNKVTYTLLMELSVKNGKMSD 312

Query: 64  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 123
           A +LF+EM + G+  +V  YT +IS   + G    AF L+DE+ ++G+ P    Y +LI 
Sbjct: 313 AEKLFDEMRERGIESDVHVYTSLISWNCRKGNMKRAFLLFDELTEKGLSPSSYTYGALID 372

Query: 124 SLHKTNPL 132
            + K   +
Sbjct: 373 GVCKVGEM 380


HSP 8 Score: 69.7 bits (169), Expect = 4.4e-11
Identity = 43/122 (35.25%), Postives = 64/122 (52.46%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           + +KG +PS  TY A I+  CK G+M  A  L+NEMQ +G+      + +LIDG    G 
Sbjct: 355 LTEKGLSPSSYTYGALIDGVCKVGEMGAAEILMNEMQSKGVNITQVVFNTLIDGYCRKGM 414

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD A  +++ M Q G   +V T   I S  ++  R DEA +    M + G+      Y++
Sbjct: 415 VDEASMIYDVMEQKGFQADVFTCNTIASCFNRLKRYDEAKQWLFRMMEGGVKLSTVSYTN 474

Query: 121 LI 123
           LI
Sbjct: 475 LI 476


HSP 9 Score: 69.3 bits (168), Expect = 5.7e-11
Identity = 35/122 (28.69%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ KG   + V +N  I+ YC+KG ++EA  + + M+++G   D +T  ++         
Sbjct: 390 MQSKGVNITQVVFNTLIDGYCRKGMVDEASMIYDVMEQKGFQADVFTCNTIASCFNRLKR 449

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            D A +    M + G+  + V+YT +I    K+G  +EA +L+ EM+ +G+ P+   Y+ 
Sbjct: 450 YDEAKQWLFRMMEGGVKLSTVSYTNLIDVYCKEGNVEEAKRLFVEMSSKGVQPNAITYNV 509

Query: 121 LI 123
           +I
Sbjct: 510 MI 511

BLAST of Cucsa.306820.2 vs. Swiss-Prot
Match: PP143_ARATH (Putative pentatricopeptide repeat-containing protein At2g02150 OS=Arabidopsis thaliana GN=At2g02150 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.5e-22
Identity = 52/122 (42.62%), Postives = 79/122 (64.75%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+  G  P+VV+Y+  ++ +CK+G M++A K   +M+  GL+P+ YTYTSLID     GN
Sbjct: 358 MKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKFYVDMRRVGLVPNEYTYTSLIDANCKIGN 417

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  L NEM Q+G+  NVVTYT +I GL    R  EA +L+ +M+  G++P+   Y++
Sbjct: 418 LSDAFRLGNEMLQVGVEWNVVTYTALIDGLCDAERMKEAEELFGKMDTAGVIPNLASYNA 477

Query: 121 LI 123
           LI
Sbjct: 478 LI 479


HSP 2 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 47/123 (38.21%), Postives = 68/123 (55.28%), Query Frame = 1

Query: 5   GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 64
           G  P+V TYN  I+  CK+G +E A  L  EM+ RGL+PDT TY S+IDG    G +D  
Sbjct: 257 GARPTVFTYNIMIDCMCKEGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGKVGRLDDT 316

Query: 65  LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIAS 124
           +  F EM  +    +V+TY  +I+   K G+     + Y EM   G+ P+   YS+L+ +
Sbjct: 317 VCFFEEMKDMCCEPDVITYNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYSTLVDA 376

Query: 125 LHK 128
             K
Sbjct: 377 FCK 379


HSP 3 Score: 85.1 bits (209), Expect = 1.0e-15
Identity = 42/122 (34.43%), Postives = 69/122 (56.56%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ +G  P  VTYN+ I+ + K G++++      EM++    PD  TY +LI+     G 
Sbjct: 288 MKFRGLVPDTVTYNSMIDGFGKVGRLDDTVCFFEEMKDMCCEPDVITYNALINCFCKFGK 347

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +   LE + EM   GL  NVV+Y+ ++    K+G   +A K Y +M + G+VP++  Y+S
Sbjct: 348 LPIGLEFYREMKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKFYVDMRRVGLVPNEYTYTS 407

Query: 121 LI 123
           LI
Sbjct: 408 LI 409


HSP 4 Score: 80.1 bits (196), Expect = 3.2e-14
Identity = 44/125 (35.20%), Postives = 68/125 (54.40%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  P+  TY + I+  CK G + +A++L NEM + G+  +  TYT+LIDG   +  
Sbjct: 393 MRRVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQVGVEWNVVTYTALIDGLCDAER 452

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A ELF +M   G+  N+ +Y  +I G  K    D A +L +E+   GI PD  +Y +
Sbjct: 453 MKEAEELFGKMDTAGVIPNLASYNALIHGFVKAKNMDRALELLNELKGRGIKPDLLLYGT 512

Query: 121 LIASL 126
            I  L
Sbjct: 513 FIWGL 517


HSP 5 Score: 79.0 bits (193), Expect = 7.2e-14
Identity = 41/118 (34.75%), Postives = 68/118 (57.63%), Query Frame = 1

Query: 5   GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 64
           G   +   + A I+  CK  ++E A  L  +M ++GL+PD   YTSL+DG    GNV  A
Sbjct: 608 GLQANAAIFTAMIDGLCKDNQVEAATTLFEQMVQKGLVPDRTAYTSLMDGNFKQGNVLEA 667

Query: 65  LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
           L L ++M ++G+  +++ YT ++ GLS   +  +A    +EM  EGI PD+ +  S++
Sbjct: 668 LALRDKMAEIGMKLDLLAYTSLVWGLSHCNQLQKARSFLEEMIGEGIHPDEVLCISVL 725


HSP 6 Score: 77.8 bits (190), Expect = 1.6e-13
Identity = 41/118 (34.75%), Postives = 68/118 (57.63%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P V+TYNA I  +CK GK+    +   EM+  GL P+  +Y++L+D     G + +A++ 
Sbjct: 330 PDVITYNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKF 389

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 126
           + +M ++GL  N  TYT +I    K G   +AF+L +EM + G+  +   Y++LI  L
Sbjct: 390 YVDMRRVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQVGVEWNVVTYTALIDGL 447


HSP 7 Score: 74.3 bits (181), Expect = 1.8e-12
Identity = 41/130 (31.54%), Postives = 73/130 (56.15%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ++ +G  P ++ Y  FI   C   K+E A  ++NEM+E G+  ++  YT+L+D    SGN
Sbjct: 498 LKGRGIKPDLLLYGTFIWGLCSLEKIEAAKVVMNEMKECGIKANSLIYTTLMDAYFKSGN 557

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKE-GIVPDDGIYS 120
               L L +EM +L +   VVT+ V+I GL K+    +A   ++ ++ + G+  +  I++
Sbjct: 558 PTEGLHLLDEMKELDIEVTVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQANAAIFT 617

Query: 121 SLIASLHKTN 130
           ++I  L K N
Sbjct: 618 AMIDGLCKDN 627


HSP 8 Score: 73.2 bits (178), Expect = 3.9e-12
Identity = 43/142 (30.28%), Postives = 73/142 (51.41%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M++ G   + + Y   ++ Y K G   E   L++EM+E  +     T+  LIDG   +  
Sbjct: 533 MKECGIKANSLIYTTLMDAYFKSGNPTEGLHLLDEMKELDIEVTVVTFCVLIDGLCKNKL 592

Query: 61  VDRALELFNEMPQ-LGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYS 120
           V +A++ FN +    GL  N   +T +I GL KD + + A  L+++M ++G+VPD   Y+
Sbjct: 593 VSKAVDYFNRISNDFGLQANAAIFTAMIDGLCKDNQVEAATTLFEQMVQKGLVPDRTAYT 652

Query: 121 SLIASLHKTNPLFPYLISRRKL 142
           SL+    K   +   L  R K+
Sbjct: 653 SLMDGNFKQGNVLEALALRDKM 674


HSP 9 Score: 70.9 bits (172), Expect = 2.0e-11
Identity = 35/108 (32.41%), Postives = 57/108 (52.78%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+  G  P++ +YNA I  + K   M+ A +L+NE++ RG+ PD   Y + I G  +   
Sbjct: 463 MDTAGVIPNLASYNALIHGFVKAKNMDRALELLNELKGRGIKPDLLLYGTFIWGLCSLEK 522

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNK 109
           ++ A  + NEM + G+  N + YT ++    K G   E   L DEM +
Sbjct: 523 IEAAKVVMNEMKECGIKANSLIYTTLMDAYFKSGNPTEGLHLLDEMKE 570


HSP 10 Score: 67.8 bits (164), Expect = 1.7e-10
Identity = 38/115 (33.04%), Postives = 56/115 (48.70%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P   + N  + R+ K GK ++  +   +M   G  P  +TY  +ID     G+V+ A  L
Sbjct: 225 PKTRSCNGLLHRFAKLGKTDDVKRFFKDMIGAGARPTVFTYNIMIDCMCKEGDVEAARGL 284

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
           F EM   GL  + VTY  +I G  K GR D+    ++EM      PD   Y++LI
Sbjct: 285 FEEMKFRGLVPDTVTYNSMIDGFGKVGRLDDTVCFFEEMKDMCCEPDVITYNALI 339


HSP 11 Score: 59.7 bits (143), Expect = 4.5e-08
Identity = 40/124 (32.26%), Postives = 61/124 (49.19%), Query Frame = 1

Query: 9   SVVTYNAFIERYCKKGKMEEAYKLINEMQ-ERGLMPDTYTYTSLIDGERASGNVDRALEL 68
           +VVT+   I+  CK   + +A    N +  + GL  +   +T++IDG      V+ A  L
Sbjct: 576 TVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQANAAIFTAMIDGLCKDNQVEAATTL 635

Query: 69  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 128
           F +M Q GL  +   YT ++ G  K G   EA  L D+M + G+  D   Y+SL+  L  
Sbjct: 636 FEQMVQKGLVPDRTAYTSLMDGNFKQGNVLEALALRDKMAEIGMKLDLLAYTSLVWGLSH 695

Query: 129 TNPL 132
            N L
Sbjct: 696 CNQL 699


HSP 12 Score: 57.8 bits (138), Expect = 1.7e-07
Identity = 34/124 (27.42%), Postives = 55/124 (44.35%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P    ++A        G +EEA +  ++M+   + P T +   L+      G  D     
Sbjct: 190 PGFGVFDALFSVLIDLGMLEEAIQCFSKMKRFRVFPKTRSCNGLLHRFAKLGKTDDVKRF 249

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 127
           F +M   G    V TY ++I  + K+G  + A  L++EM   G+VPD   Y+S+I    K
Sbjct: 250 FKDMIGAGARPTVFTYNIMIDCMCKEGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGK 309

Query: 128 TNPL 132
              L
Sbjct: 310 VGRL 313

BLAST of Cucsa.306820.2 vs. Swiss-Prot
Match: PP442_ARATH (Pentatricopeptide repeat-containing protein At5g61990, mitochondrial OS=Arabidopsis thaliana GN=At5g61990 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.2e-21
Identity = 53/129 (41.09%), Postives = 75/129 (58.14%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  KG AP V +Y   I  + K G M++A  + +EM E GL P+   Y  L+ G   SG 
Sbjct: 618 MRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTPNVIIYNMLLGGFCRSGE 677

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +++A EL +EM   GL+ N VTY  II G  K G   EAF+L+DEM  +G+VPD  +Y++
Sbjct: 678 IEKAKELLDEMSVKGLHPNAVTYCTIIDGYCKSGDLAEAFRLFDEMKLKGLVPDSFVYTT 737

Query: 121 LIASLHKTN 130
           L+    + N
Sbjct: 738 LVDGCCRLN 746


HSP 2 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 47/131 (35.88%), Postives = 74/131 (56.49%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  P+ V     I  YCKKGK+ EA      M ++G++ D  TYT L++G   +  
Sbjct: 548 MRECGVLPNKVLCTGLINEYCKKGKVIEACSAYRSMVDQGILGDAKTYTVLMNGLFKNDK 607

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD A E+F EM   G+  +V +Y V+I+G SK G   +A  ++DEM +EG+ P+  IY+ 
Sbjct: 608 VDDAEEIFREMRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTPNVIIYNM 667

Query: 121 LIASLHKTNPL 132
           L+    ++  +
Sbjct: 668 LLGGFCRSGEI 678


HSP 3 Score: 86.7 bits (213), Expect = 3.4e-16
Identity = 44/127 (34.65%), Postives = 71/127 (55.91%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M   G  P    Y + IE YC++  + + Y+L+ EM++R ++   YTY +++ G  +SG+
Sbjct: 373 MIASGLIPQAQAYASLIEGYCREKNVRQGYELLVEMKKRNIVISPYTYGTVVKGMCSSGD 432

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +D A  +  EM   G   NVV YT +I    ++ R  +A ++  EM ++GI PD   Y+S
Sbjct: 433 LDGAYNIVKEMIASGCRPNVVIYTTLIKTFLQNSRFGDAMRVLKEMKEQGIAPDIFCYNS 492

Query: 121 LIASLHK 128
           LI  L K
Sbjct: 493 LIIGLSK 499


HSP 4 Score: 80.9 bits (198), Expect = 1.9e-14
Identity = 40/131 (30.53%), Postives = 73/131 (55.73%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  +G      TY   +    K  K+++A ++  EM+ +G+ PD ++Y  LI+G    GN
Sbjct: 583 MVDQGILGDAKTYTVLMNGLFKNDKVDDAEEIFREMRGKGIAPDVFSYGVLINGFSKLGN 642

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + +A  +F+EM + GL  NV+ Y +++ G  + G  ++A +L DEM+ +G+ P+   Y +
Sbjct: 643 MQKASSIFDEMVEEGLTPNVIIYNMLLGGFCRSGEIEKAKELLDEMSVKGLHPNAVTYCT 702

Query: 121 LIASLHKTNPL 132
           +I    K+  L
Sbjct: 703 IIDGYCKSGDL 713


HSP 5 Score: 78.2 bits (191), Expect = 1.2e-13
Identity = 39/122 (31.97%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++G  P+V+ YN  +  +C+ G++E+A +L++EM  +GL P+  TY ++IDG   SG+
Sbjct: 653 MVEEGLTPNVIIYNMLLGGFCRSGEIEKAKELLDEMSVKGLHPNAVTYCTIIDGYCKSGD 712

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  LF+EM   GL  +   YT ++ G  +    + A  ++   NK+G       +++
Sbjct: 713 LAEAFRLFDEMKLKGLVPDSFVYTTLVDGCCRLNDVERAITIFG-TNKKGCASSTAPFNA 772

Query: 121 LI 123
           LI
Sbjct: 773 LI 773


HSP 6 Score: 77.8 bits (190), Expect = 1.6e-13
Identity = 43/127 (33.86%), Postives = 66/127 (51.97%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  P+  TY AFI  Y +  +   A K + EM+E G++P+    T LI+     G 
Sbjct: 513 MVENGLKPNAFTYGAFISGYIEASEFASADKYVKEMRECGVLPNKVLCTGLINEYCKKGK 572

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           V  A   +  M   G+  +  TYTV+++GL K+ + D+A +++ EM  +GI PD   Y  
Sbjct: 573 VIEACSAYRSMVDQGILGDAKTYTVLMNGLFKNDKVDDAEEIFREMRGKGIAPDVFSYGV 632

Query: 121 LIASLHK 128
           LI    K
Sbjct: 633 LINGFSK 639


HSP 7 Score: 75.9 bits (185), Expect = 6.1e-13
Identity = 42/114 (36.84%), Postives = 63/114 (55.26%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P+ VTYN  I+  CK+G +E A +L ++MQ   LMP   TYTSL++G    G       +
Sbjct: 803 PNDVTYNIMIDYLCKEGNLEAAKELFHQMQNANLMPTVITYTSLLNGYDKMGRRAEMFPV 862

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSL 122
           F+E    G+  + + Y+VII+   K+G   +A  L D+M  +  V DDG   S+
Sbjct: 863 FDEAIAAGIEPDHIMYSVIINAFLKEGMTTKALVLVDQMFAKNAV-DDGCKLSI 915


HSP 8 Score: 70.5 bits (171), Expect = 2.6e-11
Identity = 39/122 (31.97%), Postives = 62/122 (50.82%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M   G  P+VV Y   I+ + +  +  +A +++ EM+E+G+ PD + Y SLI G   +  
Sbjct: 443 MIASGCRPNVVIYTTLIKTFLQNSRFGDAMRVLKEMKEQGIAPDIFCYNSLIIGLSKAKR 502

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +D A     EM + GL  N  TY   ISG  +      A K   EM + G++P+  + + 
Sbjct: 503 MDEARSFLVEMVENGLKPNAFTYGAFISGYIEASEFASADKYVKEMRECGVLPNKVLCTG 562

Query: 121 LI 123
           LI
Sbjct: 563 LI 564


HSP 9 Score: 68.9 bits (167), Expect = 7.4e-11
Identity = 42/129 (32.56%), Postives = 65/129 (50.39%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+++G AP +  YN+ I    K  +M+EA   + EM E GL P+ +TY + I G   +  
Sbjct: 478 MKEQGIAPDIFCYNSLIIGLSKAKRMDEARSFLVEMVENGLKPNAFTYGAFISGYIEASE 537

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A +   EM + G+  N V  T +I+   K G+  EA   Y  M  +GI+ D   Y+ 
Sbjct: 538 FASADKYVKEMRECGVLPNKVLCTGLINEYCKKGKVIEACSAYRSMVDQGILGDAKTYTV 597

Query: 121 LIASLHKTN 130
           L+  L K +
Sbjct: 598 LMNGLFKND 606


HSP 10 Score: 67.4 bits (163), Expect = 2.2e-10
Identity = 37/123 (30.08%), Postives = 60/123 (48.78%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+K+    S  TY   ++  C  G ++ AY ++ EM   G  P+   YT+LI     +  
Sbjct: 408 MKKRNIVISPYTYGTVVKGMCSSGDLDGAYNIVKEMIASGCRPNVVIYTTLIKTFLQNSR 467

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A+ +  EM + G+  ++  Y  +I GLSK  R DEA     EM + G+ P+   Y +
Sbjct: 468 FGDAMRVLKEMKEQGIAPDIFCYNSLIIGLSKAKRMDEARSFLVEMVENGLKPNAFTYGA 527

Query: 121 LIA 124
            I+
Sbjct: 528 FIS 530


HSP 11 Score: 60.8 bits (146), Expect = 2.0e-08
Identity = 37/110 (33.64%), Postives = 55/110 (50.00%), Query Frame = 1

Query: 13  YNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMP 72
           Y+  I    K+G ME+A  L + M   GL+P    Y SLI+G     NV +  EL  EM 
Sbjct: 350 YDCCICVMSKEGVMEKAKALFDGMIASGLIPQAQAYASLIEGYCREKNVRQGYELLVEMK 409

Query: 73  QLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
           +  +  +  TY  ++ G+   G  D A+ +  EM   G  P+  IY++LI
Sbjct: 410 KRNIVISPYTYGTVVKGMCSSGDLDGAYNIVKEMIASGCRPNVVIYTTLI 459


HSP 12 Score: 54.3 bits (129), Expect = 1.9e-06
Identity = 33/127 (25.98%), Postives = 59/127 (46.46%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+     P+V+TY + +  Y K G+  E + + +E    G+ PD   Y+ +I+     G 
Sbjct: 831 MQNANLMPTVITYTSLLNGYDKMGRRAEMFPVFDEAIAAGIEPDHIMYSVIINAFLKEGM 890

Query: 61  VDRALELFNEM-----PQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDD 120
             +AL L ++M        G   ++ T   ++SG +K G  + A K+ + M +   +PD 
Sbjct: 891 TTKALVLVDQMFAKNAVDDGCKLSISTCRALLSGFAKVGEMEVAEKVMENMVRLQYIPDS 950

Query: 121 GIYSSLI 123
                LI
Sbjct: 951 ATVIELI 957


HSP 13 Score: 53.1 bits (126), Expect = 4.2e-06
Identity = 37/102 (36.27%), Postives = 51/102 (50.00%), Query Frame = 1

Query: 26  MEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTV 85
           ++ A KL   M  +GL+P  YTY  LIDG      ++ A  L  EM  LG++ +  TY++
Sbjct: 258 VDGALKLKESMICKGLVPLKYTYDVLIDGLCKIKRLEDAKSLLVEMDSLGVSLDNHTYSL 317

Query: 86  IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 128
           +I GL K   AD A  L  EM   GI     +Y   I  + K
Sbjct: 318 LIDGLLKGRNADAAKGLVHEMVSHGINIKPYMYDCCICVMSK 359


HSP 14 Score: 50.4 bits (119), Expect = 2.7e-05
Identity = 39/165 (23.64%), Postives = 68/165 (41.21%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYK-------------------------LINE 60
           M  KG  P+ VTY   I+ YCK G + EA++                          +N+
Sbjct: 688 MSVKGLHPNAVTYCTIIDGYCKSGDLAEAFRLFDEMKLKGLVPDSFVYTTLVDGCCRLND 747

Query: 61  MQ---------ERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR----NVVT 120
           ++         ++G    T  + +LI+     G  +   E+ N +     +R    N VT
Sbjct: 748 VERAITIFGTNKKGCASSTAPFNALINWVFKFGKTELKTEVLNRLMDGSFDRFGKPNDVT 807

Query: 121 YTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 128
           Y ++I  L K+G  + A +L+ +M    ++P    Y+SL+    K
Sbjct: 808 YNIMIDYLCKEGNLEAAKELFHQMQNANLMPTVITYTSLLNGYDK 852

BLAST of Cucsa.306820.2 vs. Swiss-Prot
Match: PP445_ARATH (Pentatricopeptide repeat-containing protein At5g65560 OS=Arabidopsis thaliana GN=At5g65560 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.1e-21
Identity = 49/118 (41.53%), Postives = 76/118 (64.41%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P VVTYN+ I+  C+ G  + AY+L++ M +RGL+PD +TYTS+ID    S  V+ A +L
Sbjct: 460 PDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDL 519

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 126
           F+ + Q G+N NVV YT +I G  K G+ DEA  + ++M  +  +P+   +++LI  L
Sbjct: 520 FDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGL 577


HSP 2 Score: 101.3 bits (251), Expect = 1.4e-20
Identity = 54/128 (42.19%), Postives = 79/128 (61.72%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M +KG  P+V+TYNA I  YCK+G +E+A  ++  M+ R L P+T TY  LI G   S N
Sbjct: 384 MLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKS-N 443

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           V +A+ + N+M +  +  +VVTY  +I G  + G  D A++L   MN  G+VPD   Y+S
Sbjct: 444 VHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTS 503

Query: 121 LIASLHKT 129
           +I SL K+
Sbjct: 504 MIDSLCKS 510


HSP 3 Score: 95.9 bits (237), Expect = 5.7e-19
Identity = 51/118 (43.22%), Postives = 70/118 (59.32%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P++ TYN  +  YCK G +EEA + ++++ E GL PD +TYTSLI G     ++D A ++
Sbjct: 216 PNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKV 275

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 126
           FNEMP  G  RN V YT +I GL    R DEA  L+ +M  +   P    Y+ LI SL
Sbjct: 276 FNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSL 333


HSP 4 Score: 91.3 bits (225), Expect = 1.4e-17
Identity = 49/122 (40.16%), Postives = 74/122 (60.66%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME +  +P+  TYN  I+ YCK   + +A  ++N+M ER ++PD  TY SLIDG+  SGN
Sbjct: 419 MESRKLSPNTRTYNELIKGYCKSN-VHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGN 478

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            D A  L + M   GL  +  TYT +I  L K  R +EA  L+D + ++G+ P+  +Y++
Sbjct: 479 FDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTA 538

Query: 121 LI 123
           LI
Sbjct: 539 LI 539


HSP 5 Score: 89.7 bits (221), Expect = 4.1e-17
Identity = 45/122 (36.89%), Postives = 72/122 (59.02%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           +E+KG  P+VV Y A I+ YCK GK++EA+ ++ +M  +  +P++ T+ +LI G  A G 
Sbjct: 523 LEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGK 582

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  L  +M ++GL   V T T++I  L KDG  D A+  + +M   G  PD   Y++
Sbjct: 583 LKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTT 642

Query: 121 LI 123
            I
Sbjct: 643 FI 644


HSP 6 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 42/122 (34.43%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P+V TY   I+  C   +  EA  L+ EM+E G+ P+ +TYT LID   +    ++A EL
Sbjct: 321 PTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKAREL 380

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 127
             +M + GL  NV+TY  +I+G  K G  ++A  + + M    + P+   Y+ LI    K
Sbjct: 381 LGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCK 440

Query: 128 TN 130
           +N
Sbjct: 441 SN 442


HSP 7 Score: 75.9 bits (185), Expect = 6.1e-13
Identity = 43/127 (33.86%), Postives = 67/127 (52.76%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  +G  P   TY + I+  CK  ++EEA  L + ++++G+ P+   YT+LIDG   +G 
Sbjct: 488 MNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGK 547

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD A  +  +M       N +T+  +I GL  DG+  EA  L ++M K G+ P     + 
Sbjct: 548 VDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTI 607

Query: 121 LIASLHK 128
           LI  L K
Sbjct: 608 LIHRLLK 614


HSP 8 Score: 75.5 bits (184), Expect = 7.9e-13
Identity = 44/121 (36.36%), Postives = 62/121 (51.24%), Query Frame = 1

Query: 5   GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 64
           G  P   TY + I  YC++  ++ A+K+ NEM  +G   +   YT LI G   +  +D A
Sbjct: 248 GLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEA 307

Query: 65  LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIAS 124
           ++LF +M        V TYTV+I  L    R  EA  L  EM + GI P+   Y+ LI S
Sbjct: 308 MDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDS 367

Query: 125 L 126
           L
Sbjct: 368 L 368


HSP 9 Score: 75.1 bits (183), Expect = 1.0e-12
Identity = 41/122 (33.61%), Postives = 69/122 (56.56%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME+ G  P++ TY   I+  C + K E+A +L+ +M E+GLMP+  TY +LI+G    G 
Sbjct: 349 MEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGM 408

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A+++   M    L+ N  TY  +I G  K     +A  + ++M +  ++PD   Y+S
Sbjct: 409 IEDAVDVVELMESRKLSPNTRTYNELIKGYCK-SNVHKAMGVLNKMLERKVLPDVVTYNS 468

Query: 121 LI 123
           LI
Sbjct: 469 LI 469


HSP 10 Score: 72.4 bits (176), Expect = 6.7e-12
Identity = 52/153 (33.99%), Postives = 73/153 (47.71%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  K   P+ +T+NA I   C  GK++EA  L  +M + GL P   T T LI      G+
Sbjct: 558 MLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGD 617

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            D A   F +M   G   +  TYT  I    ++GR  +A  +  +M + G+ PD   YSS
Sbjct: 618 FDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAKMRENGVSPDLFTYSS 677

Query: 121 LI---ASLHKTNPLFPYLISRRKLDTFREIHLH 151
           LI     L +TN  F + + +R  DT  E   H
Sbjct: 678 LIKGYGDLGQTN--FAFDVLKRMRDTGCEPSQH 708


HSP 11 Score: 67.4 bits (163), Expect = 2.2e-10
Identity = 48/164 (29.27%), Postives = 77/164 (46.95%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  KG   + V Y   I   C   +++EA  L  +M++    P   TYT LI     S  
Sbjct: 279 MPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSER 338

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              AL L  EM + G+  N+ TYTV+I  L    + ++A +L  +M ++G++P+   Y++
Sbjct: 339 KSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNA 398

Query: 121 LIASLHKTNPL-----FPYLISRRKLD----TFREIHLHFCQKN 156
           LI    K   +        L+  RKL     T+ E+   +C+ N
Sbjct: 399 LINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSN 442


HSP 12 Score: 64.3 bits (155), Expect = 1.8e-09
Identity = 34/113 (30.09%), Postives = 62/113 (54.87%), Query Frame = 1

Query: 13  YNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMP 72
           YN  +    + G ++E  ++  EM E  + P+ YTY  +++G    GNV+ A +  +++ 
Sbjct: 186 YNTLLNSLARFGLVDEMKQVYMEMLEDKVCPNIYTYNKMVNGYCKLGNVEEANQYVSKIV 245

Query: 73  QLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 126
           + GL+ +  TYT +I G  +    D AFK+++EM  +G   ++  Y+ LI  L
Sbjct: 246 EAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGL 298


HSP 13 Score: 60.5 bits (145), Expect = 2.6e-08
Identity = 35/119 (29.41%), Postives = 59/119 (49.58%), Query Frame = 1

Query: 4   KGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 63
           +G +PS + +NA +   CK  K  EA K++++M   G +P   +   LI G    G  +R
Sbjct: 790 EGISPSELVFNALLSCCCKLKKHNEAAKVVDDMICVGHLPQLESCKVLICGLYKKGEKER 849

Query: 64  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
              +F  + Q G   + + + +II G+ K G  +  ++L++ M K G       YS LI
Sbjct: 850 GTSVFQNLLQCGYYEDELAWKIIIDGVGKQGLVEAFYELFNVMEKNGCKFSSQTYSLLI 908

BLAST of Cucsa.306820.2 vs. Swiss-Prot
Match: PP407_ARATH (Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana GN=EMB2745 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.6e-21
Identity = 50/122 (40.98%), Postives = 78/122 (63.93%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M   G +PSVVTYNA I  +C  GKME+A  ++ +M+E+GL PD  +Y++++ G   S +
Sbjct: 406 MNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYD 465

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD AL +  EM + G+  + +TY+ +I G  +  R  EA  LY+EM + G+ PD+  Y++
Sbjct: 466 VDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTA 525

Query: 121 LI 123
           LI
Sbjct: 526 LI 527


HSP 2 Score: 96.3 bits (238), Expect = 4.3e-19
Identity = 50/122 (40.98%), Postives = 79/122 (64.75%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M +KG  P  +TY++ I+ +C++ + +EA  L  EM   GL PD +TYT+LI+     G+
Sbjct: 476 MVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGD 535

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +++AL+L NEM + G+  +VVTY+V+I+GL+K  R  EA +L  ++  E  VP D  Y +
Sbjct: 536 LEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHT 595

Query: 121 LI 123
           LI
Sbjct: 596 LI 597


HSP 3 Score: 95.9 bits (237), Expect = 5.7e-19
Identity = 47/127 (37.01%), Postives = 80/127 (62.99%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M++KG +P VV+Y+  +  +C+   ++EA ++  EM E+G+ PDT TY+SLI G      
Sbjct: 441 MKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRR 500

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A +L+ EM ++GL  +  TYT +I+    +G  ++A +L++EM ++G++PD   YS 
Sbjct: 501 TKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSV 560

Query: 121 LIASLHK 128
           LI  L+K
Sbjct: 561 LINGLNK 567


HSP 4 Score: 94.0 bits (232), Expect = 2.2e-18
Identity = 44/122 (36.07%), Postives = 74/122 (60.66%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  +G  P+  TY   ++ + +KG M EAY+++ EM + G  P   TY +LI+G   +G 
Sbjct: 371 MRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGK 430

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A+ +  +M + GL+ +VV+Y+ ++SG  +    DEA ++  EM ++GI PD   YSS
Sbjct: 431 MEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSS 490

Query: 121 LI 123
           LI
Sbjct: 491 LI 492


HSP 5 Score: 93.2 bits (230), Expect = 3.7e-18
Identity = 48/122 (39.34%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++G +   VTYN  I+ YCK+G   +A  +  EM   GL P   TYTSLI     +GN
Sbjct: 301 MNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGN 360

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++RA+E  ++M   GL  N  TYT ++ G S+ G  +EA+++  EMN  G  P    Y++
Sbjct: 361 MNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNA 420

Query: 121 LI 123
           LI
Sbjct: 421 LI 422


HSP 6 Score: 92.4 bits (228), Expect = 6.3e-18
Identity = 45/141 (31.91%), Postives = 82/141 (58.16%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  PSV+TY + I   CK G M  A + +++M+ RGL P+  TYT+L+DG    G 
Sbjct: 336 MLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGY 395

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A  +  EM   G + +VVTY  +I+G    G+ ++A  + ++M ++G+ PD   YS+
Sbjct: 396 MNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYST 455

Query: 121 LIASLHKTNPLFPYLISRRKL 142
           +++   ++  +   L  +R++
Sbjct: 456 VLSGFCRSYDVDEALRVKREM 476


HSP 7 Score: 89.0 bits (219), Expect = 6.9e-17
Identity = 46/127 (36.22%), Postives = 72/127 (56.69%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME KG  P+VVTYN  I+ YCK  K+++ +KL+  M  +GL P+  +Y  +I+G    G 
Sbjct: 231 METKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLISYNVVINGLCREGR 290

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +     +  EM + G + + VTY  +I G  K+G   +A  ++ EM + G+ P    Y+S
Sbjct: 291 MKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTS 350

Query: 121 LIASLHK 128
           LI S+ K
Sbjct: 351 LIHSMCK 357


HSP 8 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 43/122 (35.25%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  KG  P++++YN  I   C++G+M+E   ++ EM  RG   D  TY +LI G    GN
Sbjct: 266 MALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGN 325

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
             +AL +  EM + GL  +V+TYT +I  + K G  + A +  D+M   G+ P++  Y++
Sbjct: 326 FHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTT 385

Query: 121 LI 123
           L+
Sbjct: 386 LV 387


HSP 9 Score: 85.5 bits (210), Expect = 7.7e-16
Identity = 43/135 (31.85%), Postives = 72/135 (53.33%), Query Frame = 1

Query: 7   APSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALE 66
           +P+V TYN  I  +C  G ++ A  L ++M+ +G +P+  TY +LIDG      +D   +
Sbjct: 202 SPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFK 261

Query: 67  LFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 126
           L   M   GL  N+++Y V+I+GL ++GR  E   +  EMN+ G   D+  Y++LI    
Sbjct: 262 LLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYC 321

Query: 127 KTNPLFPYLISRRKL 142
           K       L+   ++
Sbjct: 322 KEGNFHQALVMHAEM 336


HSP 10 Score: 71.6 bits (174), Expect = 1.1e-11
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 5   GKAPSVVTYNAFIERYCK-KGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 64
           G  P V++YNA ++   + K  +  A  +  EM E  + P+ +TY  LI G   +GN+D 
Sbjct: 164 GFMPGVLSYNAVLDATIRSKRNISFAENVFKEMLESQVSPNVFTYNILIRGFCFAGNIDV 223

Query: 65  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 124
           AL LF++M   G   NVVTY  +I G  K  + D+ FKL   M  +G+ P+   Y+ +I 
Sbjct: 224 ALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLISYNVVIN 283

Query: 125 SL 126
            L
Sbjct: 284 GL 285


HSP 11 Score: 62.4 bits (150), Expect = 7.0e-09
Identity = 31/111 (27.93%), Postives = 61/111 (54.95%), Query Frame = 1

Query: 13  YNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGE-RASGNVDRALELFNEM 72
           ++  ++ Y +   +++A  +++  Q  G MP   +Y +++D   R+  N+  A  +F EM
Sbjct: 137 FDLVVKSYSRLSLIDKALSIVHLAQAHGFMPGVLSYNAVLDATIRSKRNISFAENVFKEM 196

Query: 73  PQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
            +  ++ NV TY ++I G    G  D A  L+D+M  +G +P+   Y++LI
Sbjct: 197 LESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLI 247


HSP 12 Score: 59.7 bits (143), Expect = 4.5e-08
Identity = 43/140 (30.71%), Postives = 65/140 (46.43%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  P   TY A I  YC +G +E+A +L NEM E+G++PD  TY+ LI+G      
Sbjct: 511 MLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSR 570

Query: 61  VDRA----LELFNEMPQLGLNRNVVTYTVI--------------ISGLSKDGRADEAFKL 120
              A    L+LF E     +  +V  +T+I              I G    G   EA ++
Sbjct: 571 TREAKRLLLKLFYEE---SVPSDVTYHTLIENCSNIEFKSVVSLIKGFCMKGMMTEADQV 630

Query: 121 YDEMNKEGIVPDDGIYSSLI 123
           ++ M  +   PD   Y+ +I
Sbjct: 631 FESMLGKNHKPDGTAYNIMI 647

BLAST of Cucsa.306820.2 vs. TrEMBL
Match: A0A0A0LDI0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 7.4e-42
Identity = 91/131 (69.47%), Postives = 104/131 (79.39%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M++KG      T N     +C+  + EEA +L+  M+ERG+ P+  ++    +GERASGN
Sbjct: 393 MQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSF----NGERASGN 452

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS
Sbjct: 453 VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 512

Query: 121 LIASLHKTNPL 132
           LIASLHK  PL
Sbjct: 513 LIASLHKVGPL 519

BLAST of Cucsa.306820.2 vs. TrEMBL
Match: A0A0A0LDI0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.5e-13
Identity = 46/122 (37.70%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEK     +V TY   IE Y +  K+EEA KL +EM ++G+ PD Y YTS+I+     GN
Sbjct: 253 MEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGN 312

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + RA  LF+EM +  L  N  TY  +I+G  K G    A  + ++M  +G+  +  I+++
Sbjct: 313 MKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNT 372

Query: 121 LI 123
           L+
Sbjct: 373 LM 374


HSP 2 Score: 81.3 bits (199), Expect = 1.6e-12
Identity = 46/124 (37.10%), Postives = 69/124 (55.65%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M KKG  P V  Y + I   CK G M+ A+ L +EM ER L+P+ YTY +LI+G   +G 
Sbjct: 288 MLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGE 347

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  + N+M   G++ N V +  ++ G  K G  DEA +L + M ++G    D    +
Sbjct: 348 MKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGF-EIDAFTCN 407

Query: 121 LIAS 125
           +IAS
Sbjct: 408 IIAS 410


HSP 3 Score: 77.8 bits (190), Expect = 1.8e-11
Identity = 41/113 (36.28%), Postives = 69/113 (61.06%), Query Frame = 1

Query: 10  VVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFN 69
           V ++ A ++  CKKG++  A  L++E+  +G  P   TY +L++G     +V    E+ +
Sbjct: 192 VCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILS 251

Query: 70  EMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
            M +  ++ NV TYT++I   S+  + +EA KL+DEM K+GI PD  IY+S+I
Sbjct: 252 LMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSII 304


HSP 4 Score: 74.7 bits (182), Expect = 1.5e-10
Identity = 38/114 (33.33%), Postives = 61/114 (53.51%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++   P+  TY A I   CK G+M+ A  ++N+MQ +G+  +   + +L+DG    G 
Sbjct: 323 MTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGM 382

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPD 115
           +D AL L N M Q G   +  T  +I SG  +  R +EA +L   M + G+ P+
Sbjct: 383 IDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPN 436


HSP 5 Score: 70.5 bits (171), Expect = 2.8e-09
Identity = 40/119 (33.61%), Postives = 66/119 (55.46%), Query Frame = 1

Query: 4   KGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 63
           KG  PSV+TYN  +  Y +   +    ++++ M++  +  +  TYT LI+    S  ++ 
Sbjct: 221 KGFKPSVITYNTLLNGYIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEE 280

Query: 64  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
           A +LF+EM + G+  +V  YT II+   K G    AF L+DEM +  +VP+   Y +LI
Sbjct: 281 AEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALI 339


HSP 6 Score: 67.0 bits (162), Expect = 3.1e-08
Identity = 40/127 (31.50%), Postives = 67/127 (52.76%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ KG   + V +N  ++ YCKKG ++EA +L N MQ++G   D +T   +  G   S  
Sbjct: 358 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 417

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            + A  L   M + G+  NVV++    +G    G  D A +L++EM + G+  +   Y+ 
Sbjct: 418 REEAKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTV 477

Query: 121 LIASLHK 128
           +I+ L K
Sbjct: 478 IISGLSK 480


HSP 7 Score: 177.9 bits (450), Expect = 1.3e-41
Identity = 85/126 (67.46%), Postives = 102/126 (80.95%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME+KG+ P+ VTYNA I+ Y KKGKM+EA+KL  EM+++GLMPDTYTY+SLI GE   G 
Sbjct: 377 MERKGERPNTVTYNALIDGYIKKGKMKEAHKLKKEMEDKGLMPDTYTYSSLIHGECIDGK 436

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD AL+LF+EM Q  L RNVVTYT +ISGLSKDGR DEAFKLYDEM +EG+ PDD +Y S
Sbjct: 437 VDEALKLFHEMYQRNLTRNVVTYTAMISGLSKDGRTDEAFKLYDEMKREGLTPDDRVYHS 496

Query: 121 LIASLH 127
           L+ SLH
Sbjct: 497 LVGSLH 502

BLAST of Cucsa.306820.2 vs. TrEMBL
Match: M5W590_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024892mg PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.4e-18
Identity = 57/161 (35.40%), Postives = 81/161 (50.31%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME++G AP+VV +   I+ +CK+G   EA ++I EM+ +G  P+T TY +LIDG    G 
Sbjct: 342 MEERGVAPNVVCFTTLIDIFCKEGNFVEAKQVIQEMERKGERPNTVTYNALIDGYIKKGK 401

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLY----------------- 120
           +  A +L  EM   GL  +  TY+ +I G   DG+ DEA KL+                 
Sbjct: 402 MKEAHKLKKEMEDKGLMPDTYTYSSLIHGECIDGKVDEALKLFHEMYQRNLTRNVVTYTA 461

Query: 121 ------------------DEMNKEGIVPDDGIYSSLIASLH 127
                             DEM +EG+ PDD +Y SL+ SLH
Sbjct: 462 MISGLSKDGRTDEAFKLYDEMKREGLTPDDRVYHSLVGSLH 502


HSP 2 Score: 89.4 bits (220), Expect = 5.9e-15
Identity = 50/122 (40.98%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME KG    V TY+      CK  + EEA  L+  M+ERG+ P+   +T+LID     GN
Sbjct: 307 MEGKGFQTDVFTYSTIASGLCKLNRNEEAKSLLFTMEERGVAPNVVCFTTLIDIFCKEGN 366

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A ++  EM + G   N VTY  +I G  K G+  EA KL  EM  +G++PD   YSS
Sbjct: 367 FVEAKQVIQEMERKGERPNTVTYNALIDGYIKKGKMKEAHKLKKEMEDKGLMPDTYTYSS 426

Query: 121 LI 123
           LI
Sbjct: 427 LI 428


HSP 3 Score: 82.4 bits (202), Expect = 7.2e-13
Identity = 47/122 (38.52%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEK     +  T    I+ +    K+E+A K+  E+ ERG+ PD Y YTS+I+     GN
Sbjct: 167 MEKDSVGYNAATCTLLIDWFGSCEKIEDAEKVFEEIHERGIEPDVYLYTSIINWNGRVGN 226

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + RAL LF+E+ Q GL  NV TY  +I+G  K G+   A  L +EM  +GI  +  ++++
Sbjct: 227 MKRALFLFDELTQRGLVPNVHTYGALINGACKGGQMKMADILVNEMQSKGIDVNQVVFNT 286

Query: 121 LI 123
           LI
Sbjct: 287 LI 288


HSP 4 Score: 81.6 bits (200), Expect = 1.2e-12
Identity = 46/122 (37.70%), Postives = 69/122 (56.56%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           + ++G  P+V TY A I   CK G+M+ A  L+NEMQ +G+  +   + +LIDG    G 
Sbjct: 237 LTQRGLVPNVHTYGALINGACKGGQMKMADILVNEMQSKGIDVNQVVFNTLIDGYCRKGM 296

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +D AL L + M   G   +V TY+ I SGL K  R +EA  L   M + G+ P+   +++
Sbjct: 297 MDEALRLQDFMEGKGFQTDVFTYSTIASGLCKLNRNEEAKSLLFTMEERGVAPNVVCFTT 356

Query: 121 LI 123
           LI
Sbjct: 357 LI 358


HSP 5 Score: 79.7 bits (195), Expect = 4.7e-12
Identity = 44/129 (34.11%), Postives = 69/129 (53.49%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           + ++G  P V  Y + I    + G M+ A  L +E+ +RGL+P+ +TY +LI+G    G 
Sbjct: 202 IHERGIEPDVYLYTSIINWNGRVGNMKRALFLFDELTQRGLVPNVHTYGALINGACKGGQ 261

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  L NEM   G++ N V +  +I G  + G  DEA +L D M  +G   D   YS+
Sbjct: 262 MKMADILVNEMQSKGIDVNQVVFNTLIDGYCRKGMMDEALRLQDFMEGKGFQTDVFTYST 321

Query: 121 LIASLHKTN 130
           + + L K N
Sbjct: 322 IASGLCKLN 330


HSP 6 Score: 74.7 bits (182), Expect = 1.5e-10
Identity = 40/122 (32.79%), Postives = 67/122 (54.92%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ KG   + V +N  I+ YC+KG M+EA +L + M+ +G   D +TY+++  G      
Sbjct: 272 MQSKGIDVNQVVFNTLIDGYCRKGMMDEALRLQDFMEGKGFQTDVFTYSTIASGLCKLNR 331

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            + A  L   M + G+  NVV +T +I    K+G   EA ++  EM ++G  P+   Y++
Sbjct: 332 NEEAKSLLFTMEERGVAPNVVCFTTLIDIFCKEGNFVEAKQVIQEMERKGERPNTVTYNA 391

Query: 121 LI 123
           LI
Sbjct: 392 LI 393


HSP 7 Score: 58.5 bits (140), Expect = 1.1e-05
Identity = 32/122 (26.23%), Postives = 65/122 (53.28%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M +KG   +V +    +   CK+G++++A  L+ EM  R + P+  TY +L+       +
Sbjct: 97  MVEKGVQITVHSLTLVMNELCKRGEVKKAKALMGEMAGRQVKPNVVTYNTLLKAYIERKD 156

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            +   E  + M +  +  N  T T++I       + ++A K+++E+++ GI PD  +Y+S
Sbjct: 157 FEGVNEALSLMEKDSVGYNAATCTLLIDWFGSCEKIEDAEKVFEEIHERGIEPDVYLYTS 216

Query: 121 LI 123
           +I
Sbjct: 217 II 218


HSP 8 Score: 167.9 bits (424), Expect = 1.3e-38
Identity = 79/126 (62.70%), Postives = 100/126 (79.37%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME+KG  P+++TYN  I+ Y K+G M+EA+KL +E++ RGL+PD YT TSLI GE   G 
Sbjct: 491 MEEKGNVPNIITYNVLIDGYSKRGNMKEAHKLKDELENRGLIPDVYTCTSLIHGECIDGK 550

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD AL+LF+EMPQ GL  NVVTYT +ISGLSKDGR++EAFKLYDEM + G+ PDD +YSS
Sbjct: 551 VDMALKLFDEMPQRGLVPNVVTYTAMISGLSKDGRSEEAFKLYDEMKETGLTPDDTVYSS 610

Query: 121 LIASLH 127
           L+ SLH
Sbjct: 611 LVGSLH 616

BLAST of Cucsa.306820.2 vs. TrEMBL
Match: F6HR46_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0040g01530 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.7e-17
Identity = 49/127 (38.58%), Postives = 79/127 (62.20%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++G +P+ +++   I+ YCK+G   EA ++  EM+E+G +P+  TY  LIDG    GN
Sbjct: 456 MVERGVSPNTMSFTTLIDIYCKEGNFVEAKRVFREMEEKGNVPNIITYNVLIDGYSKRGN 515

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A +L +E+   GL  +V T T +I G   DG+ D A KL+DEM + G+VP+   Y++
Sbjct: 516 MKEAHKLKDELENRGLIPDVYTCTSLIHGECIDGKVDMALKLFDEMPQRGLVPNVVTYTA 575

Query: 121 LIASLHK 128
           +I+ L K
Sbjct: 576 MISGLSK 582


HSP 2 Score: 95.9 bits (237), Expect = 6.3e-17
Identity = 54/122 (44.26%), Postives = 75/122 (61.48%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEK+G A +VVTY   I  +   GK+EEA +L  EM+E+G+  D Y YTS+I     SGN
Sbjct: 281 MEKEGVACNVVTYTLLIHGFSNIGKIEEAQRLFEEMREKGIEADVYVYTSIISCNCRSGN 340

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           V RAL LF+EM   GL  +  TY  +I G+ K G+ + A  L +EM  +GI  +  I+++
Sbjct: 341 VKRALVLFDEMTDKGLIPSAHTYGALIHGVCKAGQMEAAQMLVNEMQGKGIDLNPVIFNT 400

Query: 121 LI 123
           LI
Sbjct: 401 LI 402


HSP 3 Score: 94.4 bits (233), Expect = 1.8e-16
Identity = 51/122 (41.80%), Postives = 69/122 (56.56%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEKKG    V  YN+     CK  + +EA  L+  M ERG+ P+T ++T+LID     GN
Sbjct: 421 MEKKGLESDVFAYNSIASGLCKLNRKDEAKGLLFSMVERGVSPNTMSFTTLIDIYCKEGN 480

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A  +F EM + G   N++TY V+I G SK G   EA KL DE+   G++PD    +S
Sbjct: 481 FVEAKRVFREMEEKGNVPNIITYNVLIDGYSKRGNMKEAHKLKDELENRGLIPDVYTCTS 540

Query: 121 LI 123
           LI
Sbjct: 541 LI 542


HSP 4 Score: 86.3 bits (212), Expect = 5.0e-14
Identity = 52/122 (42.62%), Postives = 67/122 (54.92%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  KG  PS  TY A I   CK G+ME A  L+NEMQ +G+  +   + +LIDG   SG 
Sbjct: 351 MTDKGLIPSAHTYGALIHGVCKAGQMEAAQMLVNEMQGKGIDLNPVIFNTLIDGYCESGM 410

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD AL L   M + GL  +V  Y  I SGL K  R DEA  L   M + G+ P+   +++
Sbjct: 411 VDEALRLQVVMEKKGLESDVFAYNSIASGLCKLNRKDEAKGLLFSMVERGVSPNTMSFTT 470

Query: 121 LI 123
           LI
Sbjct: 471 LI 472


HSP 5 Score: 85.1 bits (209), Expect = 1.1e-13
Identity = 45/129 (34.88%), Postives = 70/129 (54.26%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M +KG    V  Y + I   C+ G ++ A  L +EM ++GL+P  +TY +LI G   +G 
Sbjct: 316 MREKGIEADVYVYTSIISCNCRSGNVKRALVLFDEMTDKGLIPSAHTYGALIHGVCKAGQ 375

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A  L NEM   G++ N V +  +I G  + G  DEA +L   M K+G+  D   Y+S
Sbjct: 376 MEAAQMLVNEMQGKGIDLNPVIFNTLIDGYCESGMVDEALRLQVVMEKKGLESDVFAYNS 435

Query: 121 LIASLHKTN 130
           + + L K N
Sbjct: 436 IASGLCKLN 444


HSP 6 Score: 84.3 bits (207), Expect = 1.9e-13
Identity = 46/128 (35.94%), Postives = 69/128 (53.91%), Query Frame = 1

Query: 4   KGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 63
           KG   +VVTYNAFIE Y K+  +    +++  M++ G+  +  TYT LI G    G ++ 
Sbjct: 249 KGVKANVVTYNAFIEGYFKRLDLGGVAEILTLMEKEGVACNVVTYTLLIHGFSNIGKIEE 308

Query: 64  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 123
           A  LF EM + G+  +V  YT IIS   + G    A  L+DEM  +G++P    Y +LI 
Sbjct: 309 AQRLFEEMREKGIEADVYVYTSIISCNCRSGNVKRALVLFDEMTDKGLIPSAHTYGALIH 368

Query: 124 SLHKTNPL 132
            + K   +
Sbjct: 369 GVCKAGQM 376


HSP 7 Score: 73.6 bits (179), Expect = 3.4e-10
Identity = 37/115 (32.17%), Postives = 69/115 (60.00%), Query Frame = 1

Query: 9   SVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELF 68
           +V +    ++  CK+G +E   KL++E+  +G+  +  TY + I+G     ++    E+ 
Sbjct: 219 TVYSLTIVLDGLCKRGDVEMGRKLMDEVAAKGVKANVVTYNAFIEGYFKRLDLGGVAEIL 278

Query: 69  NEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 124
             M + G+  NVVTYT++I G S  G+ +EA +L++EM ++GI  D  +Y+S+I+
Sbjct: 279 TLMEKEGVACNVVTYTLLIHGFSNIGKIEEAQRLFEEMREKGIEADVYVYTSIIS 333


HSP 8 Score: 70.5 bits (171), Expect = 2.8e-09
Identity = 40/127 (31.50%), Postives = 68/127 (53.54%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ KG   + V +N  I+ YC+ G ++EA +L   M+++GL  D + Y S+  G      
Sbjct: 386 MQGKGIDLNPVIFNTLIDGYCESGMVDEALRLQVVMEKKGLESDVFAYNSIASGLCKLNR 445

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            D A  L   M + G++ N +++T +I    K+G   EA +++ EM ++G VP+   Y+ 
Sbjct: 446 KDEAKGLLFSMVERGVSPNTMSFTTLIDIYCKEGNFVEAKRVFREMEEKGNVPNIITYNV 505

Query: 121 LIASLHK 128
           LI    K
Sbjct: 506 LIDGYSK 512


HSP 9 Score: 46.2 bits (108), Expect = 5.7e-02
Identity = 28/108 (25.93%), Postives = 46/108 (42.59%), Query Frame = 1

Query: 20  YCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRN 79
           Y       EA +    M+++G   D  +    +   R  G VD  L  F  M  L +   
Sbjct: 160 YADNKMFGEALEAFEHMEKKGFHIDERSCLVYLLALRRGGQVDSCLRFFRRMVNLDVEVT 219

Query: 80  VVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 128
           V + T+++ GL K G  +   KL DE+  +G+  +   Y++ I    K
Sbjct: 220 VYSLTIVLDGLCKRGDVEMGRKLMDEVAAKGVKANVVTYNAFIEGYFK 267


HSP 10 Score: 45.1 bits (105), Expect = 1.3e-01
Identity = 31/122 (25.41%), Postives = 54/122 (44.26%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEKKG      +   ++    + G+++   +    M    +    Y+ T ++DG    G+
Sbjct: 176 MEKKGFHIDERSCLVYLLALRRGGQVDSCLRFFRRMVNLDVEVTVYSLTIVLDGLCKRGD 235

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           V+   +L +E+   G+  NVVTY   I G  K        ++   M KEG+  +   Y+ 
Sbjct: 236 VEMGRKLMDEVAAKGVKANVVTYNAFIEGYFKRLDLGGVAEILTLMEKEGVACNVVTYTL 295

Query: 121 LI 123
           LI
Sbjct: 296 LI 297


HSP 11 Score: 166.8 bits (421), Expect = 2.9e-38
Identity = 77/127 (60.63%), Postives = 101/127 (79.53%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEKKG+ P+++TYN  I+ YCKK K+++A+K+ +EM  +GL+PD YTY+SLI GE   G 
Sbjct: 488 MEKKGEVPNIITYNTLIDAYCKKEKVKQAHKIKSEMINKGLLPDLYTYSSLIHGECIVGR 547

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD AL+LFNEM   G+ RNV TYT +ISGLSK+GRADEAFKLYDEM K G++PDD +++S
Sbjct: 548 VDEALKLFNEMRLKGITRNVATYTSMISGLSKEGRADEAFKLYDEMMKIGLIPDDRVFTS 607

Query: 121 LIASLHK 128
           L+ S HK
Sbjct: 608 LVGSFHK 614

BLAST of Cucsa.306820.2 vs. TrEMBL
Match: G7I783_MEDTR (PPR containing plant-like protein OS=Medicago truncatula GN=MTR_1g045880 PE=4 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 3.7e-17
Identity = 51/127 (40.16%), Postives = 79/127 (62.20%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M +KG  P+VVT+  FIE YCK+G + EA +L  +M+++G +P+  TY +LID       
Sbjct: 453 MVEKGVKPNVVTFTMFIEIYCKEGNLAEAERLFRDMEKKGEVPNIITYNTLIDAYCKKEK 512

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           V +A ++ +EM   GL  ++ TY+ +I G    GR DEA KL++EM  +GI  +   Y+S
Sbjct: 513 VKQAHKIKSEMINKGLLPDLYTYSSLIHGECIVGRVDEALKLFNEMRLKGITRNVATYTS 572

Query: 121 LIASLHK 128
           +I+ L K
Sbjct: 573 MISGLSK 579


HSP 2 Score: 84.0 bits (206), Expect = 2.5e-13
Identity = 44/122 (36.07%), Postives = 73/122 (59.84%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ KG   ++V +N  ++ YC++GKM+EA +L   M+++G+  D +TY  L +G      
Sbjct: 383 MQSKGVDLNLVIFNTTMDGYCRRGKMDEALRLQAIMEKKGINADVFTYNILANGLCKLHR 442

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            D A  + N M + G+  NVVT+T+ I    K+G   EA +L+ +M K+G VP+   Y++
Sbjct: 443 YDEAKCILNSMVEKGVKPNVVTFTMFIEIYCKEGNLAEAERLFRDMEKKGEVPNIITYNT 502

Query: 121 LI 123
           LI
Sbjct: 503 LI 504


HSP 3 Score: 83.6 bits (205), Expect = 3.2e-13
Identity = 45/122 (36.89%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEKKG    V TYN      CK  + +EA  ++N M E+G+ P+  T+T  I+     GN
Sbjct: 418 MEKKGINADVFTYNILANGLCKLHRYDEAKCILNSMVEKGVKPNVVTFTMFIEIYCKEGN 477

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  LF +M + G   N++TY  +I    K  +  +A K+  EM  +G++PD   YSS
Sbjct: 478 LAEAERLFRDMEKKGEVPNIITYNTLIDAYCKKEKVKQAHKIKSEMINKGLLPDLYTYSS 537

Query: 121 LI 123
           LI
Sbjct: 538 LI 539


HSP 4 Score: 72.0 bits (175), Expect = 9.8e-10
Identity = 40/114 (35.09%), Postives = 64/114 (56.14%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++   P+  TY A I   CK G+ME A  L+ EMQ +G+  +   + + +DG    G 
Sbjct: 348 MSQRDIVPNAHTYGALIGGVCKAGQMEAAEILLLEMQSKGVDLNLVIFNTTMDGYCRRGK 407

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPD 115
           +D AL L   M + G+N +V TY ++ +GL K  R DEA  + + M ++G+ P+
Sbjct: 408 MDEALRLQAIMEKKGINADVFTYNILANGLCKLHRYDEAKCILNSMVEKGVKPN 461


HSP 5 Score: 70.9 bits (172), Expect = 2.2e-09
Identity = 41/127 (32.28%), Postives = 67/127 (52.76%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M +K     V  Y++ I    + G M+ A+ L +EM +R ++P+ +TY +LI G   +G 
Sbjct: 313 MREKNIEMDVYVYSSMISWSRRLGNMKRAFALFDEMSQRDIVPNAHTYGALIGGVCKAGQ 372

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A  L  EM   G++ N+V +   + G  + G+ DEA +L   M K+GI  D   Y+ 
Sbjct: 373 MEAAEILLLEMQSKGVDLNLVIFNTTMDGYCRRGKMDEALRLQAIMEKKGINADVFTYNI 432

Query: 121 LIASLHK 128
           L   L K
Sbjct: 433 LANGLCK 439


HSP 6 Score: 67.0 bits (162), Expect = 3.1e-08
Identity = 35/124 (28.23%), Postives = 66/124 (53.23%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P+V TYN  +  Y  +   +   +++  M++  ++    TY+ LI    +SG+++ A ++
Sbjct: 250 PNVFTYNTLLNAYVGRKDRKGVDEILKLMEKEQVVFSVATYSILIQWYSSSGDIEEAEKI 309

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 127
           F EM +  +  +V  Y+ +IS   + G    AF L+DEM++  IVP+   Y +LI  + K
Sbjct: 310 FEEMREKNIEMDVYVYSSMISWSRRLGNMKRAFALFDEMSQRDIVPNAHTYGALIGGVCK 369

Query: 128 TNPL 132
              +
Sbjct: 370 AGQM 373


HSP 7 Score: 166.8 bits (421), Expect = 2.9e-38
Identity = 75/126 (59.52%), Postives = 104/126 (82.54%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME KG+ P+VVTYNAFI+ YCKKG M+EA+K+ +EM+++ +MPD YT+T L+ GE  +G 
Sbjct: 481 MESKGERPNVVTYNAFIDGYCKKGLMKEAHKVRSEMEDKEVMPDVYTFTCLVHGECMAGR 540

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD A++LF+EMPQ G  RNV++YT +I+GLSK+GR++EAF+LYDEM   GI+PDD +YS+
Sbjct: 541 VDDAMKLFDEMPQRGFVRNVISYTAMIAGLSKEGRSEEAFRLYDEMKNAGILPDDTLYST 600

Query: 121 LIASLH 127
           L+ SLH
Sbjct: 601 LVGSLH 606

BLAST of Cucsa.306820.2 vs. TAIR10
Match: AT2G32630.1 (AT2G32630.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 142.9 bits (359), Expect = 2.3e-34
Identity = 69/126 (54.76%), Postives = 92/126 (73.02%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  KG  P+ +TYN  I  YCK+GK++EA KL   M+  G+ PD+YTYTSLI GE  + N
Sbjct: 495 MSSKGVQPNAITYNVMIYAYCKQGKIKEARKLRANMEANGMDPDSYTYTSLIHGECIADN 554

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD A+ LF+EM   GL++N VTYTV+ISGLSK G++DEAF LYDEM ++G   D+ +Y++
Sbjct: 555 VDEAMRLFSEMGLKGLDQNSVTYTVMISGLSKAGKSDEAFGLYDEMKRKGYTIDNKVYTA 614

Query: 121 LIASLH 127
           LI S+H
Sbjct: 615 LIGSMH 620


HSP 2 Score: 85.5 bits (210), Expect = 4.3e-17
Identity = 47/122 (38.52%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+K G   + VTY   +E   K GKM +A KL +EM+ERG+  D + YTSLI      GN
Sbjct: 285 MKKDGVVYNKVTYTLLMELSVKNGKMSDAEKLFDEMRERGIESDVHVYTSLISWNCRKGN 344

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + RA  LF+E+ + GL+ +  TY  +I G+ K G    A  L +EM  +G+     ++++
Sbjct: 345 MKRAFLLFDELTEKGLSPSSYTYGALIDGVCKVGEMGAAEILMNEMQSKGVNITQVVFNT 404

Query: 121 LI 123
           LI
Sbjct: 405 LI 406


HSP 3 Score: 84.3 bits (207), Expect = 9.6e-17
Identity = 43/114 (37.72%), Postives = 62/114 (54.39%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++G    V  Y + I   C+KG M+ A+ L +E+ E+GL P +YTY +LIDG    G 
Sbjct: 320 MRERGIESDVHVYTSLISWNCRKGNMKRAFLLFDELTEKGLSPSSYTYGALIDGVCKVGE 379

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPD 115
           +  A  L NEM   G+N   V +  +I G  + G  DEA  +YD M ++G   D
Sbjct: 380 MGAAEILMNEMQSKGVNITQVVFNTLIDGYCRKGMVDEASMIYDVMEQKGFQAD 433


HSP 4 Score: 77.4 bits (189), Expect = 1.2e-14
Identity = 46/122 (37.70%), Postives = 63/122 (51.64%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME+KG    V T N     + +  + +EA + +  M E G+   T +YT+LID     GN
Sbjct: 425 MEQKGFQADVFTCNTIASCFNRLKRYDEAKQWLFRMMEGGVKLSTVSYTNLIDVYCKEGN 484

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           V+ A  LF EM   G+  N +TY V+I    K G+  EA KL   M   G+ PD   Y+S
Sbjct: 485 VEEAKRLFVEMSSKGVQPNAITYNVMIYAYCKQGKIKEARKLRANMEANGMDPDSYTYTS 544

Query: 121 LI 123
           LI
Sbjct: 545 LI 546


HSP 5 Score: 74.7 bits (182), Expect = 7.6e-14
Identity = 42/127 (33.07%), Postives = 66/127 (51.97%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G   S V+Y   I+ YCK+G +EEA +L  EM  +G+ P+  TY  +I      G 
Sbjct: 460 MMEGGVKLSTVSYTNLIDVYCKEGNVEEAKRLFVEMSSKGVQPNAITYNVMIYAYCKQGK 519

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A +L   M   G++ +  TYT +I G       DEA +L+ EM  +G+  +   Y+ 
Sbjct: 520 IKEARKLRANMEANGMDPDSYTYTSLIHGECIADNVDEAMRLFSEMGLKGLDQNSVTYTV 579

Query: 121 LIASLHK 128
           +I+ L K
Sbjct: 580 MISGLSK 586


HSP 6 Score: 74.7 bits (182), Expect = 7.6e-14
Identity = 42/126 (33.33%), Postives = 74/126 (58.73%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDG---ERA 60
           M   G   +V +    +E  C++G++E++ KLI E   +G+ P+ YTY ++I+    +R 
Sbjct: 215 MVDSGVKITVYSLTIVVEGLCRRGEVEKSKKLIKEFSVKGIKPEAYTYNTIINAYVKQRD 274

Query: 61  SGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGI 120
              V+  L++   M + G+  N VTYT+++    K+G+  +A KL+DEM + GI  D  +
Sbjct: 275 FSGVEGVLKV---MKKDGVVYNKVTYTLLMELSVKNGKMSDAEKLFDEMRERGIESDVHV 334

Query: 121 YSSLIA 124
           Y+SLI+
Sbjct: 335 YTSLIS 337


HSP 7 Score: 71.2 bits (173), Expect = 8.4e-13
Identity = 39/128 (30.47%), Postives = 66/128 (51.56%), Query Frame = 1

Query: 4   KGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 63
           KG  P   TYN  I  Y K+        ++  M++ G++ +  TYT L++    +G +  
Sbjct: 253 KGIKPEAYTYNTIINAYVKQRDFSGVEGVLKVMKKDGVVYNKVTYTLLMELSVKNGKMSD 312

Query: 64  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 123
           A +LF+EM + G+  +V  YT +IS   + G    AF L+DE+ ++G+ P    Y +LI 
Sbjct: 313 AEKLFDEMRERGIESDVHVYTSLISWNCRKGNMKRAFLLFDELTEKGLSPSSYTYGALID 372

Query: 124 SLHKTNPL 132
            + K   +
Sbjct: 373 GVCKVGEM 380


HSP 8 Score: 69.7 bits (169), Expect = 2.5e-12
Identity = 43/122 (35.25%), Postives = 64/122 (52.46%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           + +KG +PS  TY A I+  CK G+M  A  L+NEMQ +G+      + +LIDG    G 
Sbjct: 355 LTEKGLSPSSYTYGALIDGVCKVGEMGAAEILMNEMQSKGVNITQVVFNTLIDGYCRKGM 414

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD A  +++ M Q G   +V T   I S  ++  R DEA +    M + G+      Y++
Sbjct: 415 VDEASMIYDVMEQKGFQADVFTCNTIASCFNRLKRYDEAKQWLFRMMEGGVKLSTVSYTN 474

Query: 121 LI 123
           LI
Sbjct: 475 LI 476


HSP 9 Score: 69.3 bits (168), Expect = 3.2e-12
Identity = 35/122 (28.69%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ KG   + V +N  I+ YC+KG ++EA  + + M+++G   D +T  ++         
Sbjct: 390 MQSKGVNITQVVFNTLIDGYCRKGMVDEASMIYDVMEQKGFQADVFTCNTIASCFNRLKR 449

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            D A +    M + G+  + V+YT +I    K+G  +EA +L+ EM+ +G+ P+   Y+ 
Sbjct: 450 YDEAKQWLFRMMEGGVKLSTVSYTNLIDVYCKEGNVEEAKRLFVEMSSKGVQPNAITYNV 509

Query: 121 LI 123
           +I
Sbjct: 510 MI 511

BLAST of Cucsa.306820.2 vs. TAIR10
Match: AT2G02150.1 (AT2G02150.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 105.9 bits (263), Expect = 3.1e-23
Identity = 52/122 (42.62%), Postives = 79/122 (64.75%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+  G  P+VV+Y+  ++ +CK+G M++A K   +M+  GL+P+ YTYTSLID     GN
Sbjct: 358 MKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKFYVDMRRVGLVPNEYTYTSLIDANCKIGN 417

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  L NEM Q+G+  NVVTYT +I GL    R  EA +L+ +M+  G++P+   Y++
Sbjct: 418 LSDAFRLGNEMLQVGVEWNVVTYTALIDGLCDAERMKEAEELFGKMDTAGVIPNLASYNA 477

Query: 121 LI 123
           LI
Sbjct: 478 LI 479


HSP 2 Score: 88.2 bits (217), Expect = 6.7e-18
Identity = 47/123 (38.21%), Postives = 68/123 (55.28%), Query Frame = 1

Query: 5   GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 64
           G  P+V TYN  I+  CK+G +E A  L  EM+ RGL+PDT TY S+IDG    G +D  
Sbjct: 257 GARPTVFTYNIMIDCMCKEGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGKVGRLDDT 316

Query: 65  LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIAS 124
           +  F EM  +    +V+TY  +I+   K G+     + Y EM   G+ P+   YS+L+ +
Sbjct: 317 VCFFEEMKDMCCEPDVITYNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYSTLVDA 376

Query: 125 LHK 128
             K
Sbjct: 377 FCK 379


HSP 3 Score: 85.1 bits (209), Expect = 5.6e-17
Identity = 42/122 (34.43%), Postives = 69/122 (56.56%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ +G  P  VTYN+ I+ + K G++++      EM++    PD  TY +LI+     G 
Sbjct: 288 MKFRGLVPDTVTYNSMIDGFGKVGRLDDTVCFFEEMKDMCCEPDVITYNALINCFCKFGK 347

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +   LE + EM   GL  NVV+Y+ ++    K+G   +A K Y +M + G+VP++  Y+S
Sbjct: 348 LPIGLEFYREMKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKFYVDMRRVGLVPNEYTYTS 407

Query: 121 LI 123
           LI
Sbjct: 408 LI 409


HSP 4 Score: 80.1 bits (196), Expect = 1.8e-15
Identity = 44/125 (35.20%), Postives = 68/125 (54.40%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  P+  TY + I+  CK G + +A++L NEM + G+  +  TYT+LIDG   +  
Sbjct: 393 MRRVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQVGVEWNVVTYTALIDGLCDAER 452

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A ELF +M   G+  N+ +Y  +I G  K    D A +L +E+   GI PD  +Y +
Sbjct: 453 MKEAEELFGKMDTAGVIPNLASYNALIHGFVKAKNMDRALELLNELKGRGIKPDLLLYGT 512

Query: 121 LIASL 126
            I  L
Sbjct: 513 FIWGL 517


HSP 5 Score: 79.0 bits (193), Expect = 4.0e-15
Identity = 41/118 (34.75%), Postives = 68/118 (57.63%), Query Frame = 1

Query: 5   GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 64
           G   +   + A I+  CK  ++E A  L  +M ++GL+PD   YTSL+DG    GNV  A
Sbjct: 608 GLQANAAIFTAMIDGLCKDNQVEAATTLFEQMVQKGLVPDRTAYTSLMDGNFKQGNVLEA 667

Query: 65  LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
           L L ++M ++G+  +++ YT ++ GLS   +  +A    +EM  EGI PD+ +  S++
Sbjct: 668 LALRDKMAEIGMKLDLLAYTSLVWGLSHCNQLQKARSFLEEMIGEGIHPDEVLCISVL 725


HSP 6 Score: 77.8 bits (190), Expect = 9.0e-15
Identity = 41/118 (34.75%), Postives = 68/118 (57.63%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P V+TYNA I  +CK GK+    +   EM+  GL P+  +Y++L+D     G + +A++ 
Sbjct: 330 PDVITYNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKF 389

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 126
           + +M ++GL  N  TYT +I    K G   +AF+L +EM + G+  +   Y++LI  L
Sbjct: 390 YVDMRRVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQVGVEWNVVTYTALIDGL 447


HSP 7 Score: 74.3 bits (181), Expect = 1.0e-13
Identity = 41/130 (31.54%), Postives = 73/130 (56.15%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ++ +G  P ++ Y  FI   C   K+E A  ++NEM+E G+  ++  YT+L+D    SGN
Sbjct: 498 LKGRGIKPDLLLYGTFIWGLCSLEKIEAAKVVMNEMKECGIKANSLIYTTLMDAYFKSGN 557

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKE-GIVPDDGIYS 120
               L L +EM +L +   VVT+ V+I GL K+    +A   ++ ++ + G+  +  I++
Sbjct: 558 PTEGLHLLDEMKELDIEVTVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQANAAIFT 617

Query: 121 SLIASLHKTN 130
           ++I  L K N
Sbjct: 618 AMIDGLCKDN 627


HSP 8 Score: 73.2 bits (178), Expect = 2.2e-13
Identity = 43/142 (30.28%), Postives = 73/142 (51.41%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M++ G   + + Y   ++ Y K G   E   L++EM+E  +     T+  LIDG   +  
Sbjct: 533 MKECGIKANSLIYTTLMDAYFKSGNPTEGLHLLDEMKELDIEVTVVTFCVLIDGLCKNKL 592

Query: 61  VDRALELFNEMPQ-LGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYS 120
           V +A++ FN +    GL  N   +T +I GL KD + + A  L+++M ++G+VPD   Y+
Sbjct: 593 VSKAVDYFNRISNDFGLQANAAIFTAMIDGLCKDNQVEAATTLFEQMVQKGLVPDRTAYT 652

Query: 121 SLIASLHKTNPLFPYLISRRKL 142
           SL+    K   +   L  R K+
Sbjct: 653 SLMDGNFKQGNVLEALALRDKM 674


HSP 9 Score: 70.9 bits (172), Expect = 1.1e-12
Identity = 35/108 (32.41%), Postives = 57/108 (52.78%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+  G  P++ +YNA I  + K   M+ A +L+NE++ RG+ PD   Y + I G  +   
Sbjct: 463 MDTAGVIPNLASYNALIHGFVKAKNMDRALELLNELKGRGIKPDLLLYGTFIWGLCSLEK 522

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNK 109
           ++ A  + NEM + G+  N + YT ++    K G   E   L DEM +
Sbjct: 523 IEAAKVVMNEMKECGIKANSLIYTTLMDAYFKSGNPTEGLHLLDEMKE 570


HSP 10 Score: 67.8 bits (164), Expect = 9.3e-12
Identity = 38/115 (33.04%), Postives = 56/115 (48.70%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P   + N  + R+ K GK ++  +   +M   G  P  +TY  +ID     G+V+ A  L
Sbjct: 225 PKTRSCNGLLHRFAKLGKTDDVKRFFKDMIGAGARPTVFTYNIMIDCMCKEGDVEAARGL 284

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
           F EM   GL  + VTY  +I G  K GR D+    ++EM      PD   Y++LI
Sbjct: 285 FEEMKFRGLVPDTVTYNSMIDGFGKVGRLDDTVCFFEEMKDMCCEPDVITYNALI 339


HSP 11 Score: 59.7 bits (143), Expect = 2.5e-09
Identity = 40/124 (32.26%), Postives = 61/124 (49.19%), Query Frame = 1

Query: 9   SVVTYNAFIERYCKKGKMEEAYKLINEMQ-ERGLMPDTYTYTSLIDGERASGNVDRALEL 68
           +VVT+   I+  CK   + +A    N +  + GL  +   +T++IDG      V+ A  L
Sbjct: 576 TVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQANAAIFTAMIDGLCKDNQVEAATTL 635

Query: 69  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 128
           F +M Q GL  +   YT ++ G  K G   EA  L D+M + G+  D   Y+SL+  L  
Sbjct: 636 FEQMVQKGLVPDRTAYTSLMDGNFKQGNVLEALALRDKMAEIGMKLDLLAYTSLVWGLSH 695

Query: 129 TNPL 132
            N L
Sbjct: 696 CNQL 699


HSP 12 Score: 57.8 bits (138), Expect = 9.7e-09
Identity = 34/124 (27.42%), Postives = 55/124 (44.35%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P    ++A        G +EEA +  ++M+   + P T +   L+      G  D     
Sbjct: 190 PGFGVFDALFSVLIDLGMLEEAIQCFSKMKRFRVFPKTRSCNGLLHRFAKLGKTDDVKRF 249

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 127
           F +M   G    V TY ++I  + K+G  + A  L++EM   G+VPD   Y+S+I    K
Sbjct: 250 FKDMIGAGARPTVFTYNIMIDCMCKEGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGK 309

Query: 128 TNPL 132
              L
Sbjct: 310 VGRL 313

BLAST of Cucsa.306820.2 vs. TAIR10
Match: AT5G61990.1 (AT5G61990.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 104.8 bits (260), Expect = 6.9e-23
Identity = 53/129 (41.09%), Postives = 75/129 (58.14%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  KG AP V +Y   I  + K G M++A  + +EM E GL P+   Y  L+ G   SG 
Sbjct: 618 MRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTPNVIIYNMLLGGFCRSGE 677

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +++A EL +EM   GL+ N VTY  II G  K G   EAF+L+DEM  +G+VPD  +Y++
Sbjct: 678 IEKAKELLDEMSVKGLHPNAVTYCTIIDGYCKSGDLAEAFRLFDEMKLKGLVPDSFVYTT 737

Query: 121 LIASLHKTN 130
           L+    + N
Sbjct: 738 LVDGCCRLN 746


HSP 2 Score: 87.8 bits (216), Expect = 8.7e-18
Identity = 47/131 (35.88%), Postives = 74/131 (56.49%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  P+ V     I  YCKKGK+ EA      M ++G++ D  TYT L++G   +  
Sbjct: 548 MRECGVLPNKVLCTGLINEYCKKGKVIEACSAYRSMVDQGILGDAKTYTVLMNGLFKNDK 607

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD A E+F EM   G+  +V +Y V+I+G SK G   +A  ++DEM +EG+ P+  IY+ 
Sbjct: 608 VDDAEEIFREMRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTPNVIIYNM 667

Query: 121 LIASLHKTNPL 132
           L+    ++  +
Sbjct: 668 LLGGFCRSGEI 678


HSP 3 Score: 86.7 bits (213), Expect = 1.9e-17
Identity = 44/127 (34.65%), Postives = 71/127 (55.91%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M   G  P    Y + IE YC++  + + Y+L+ EM++R ++   YTY +++ G  +SG+
Sbjct: 373 MIASGLIPQAQAYASLIEGYCREKNVRQGYELLVEMKKRNIVISPYTYGTVVKGMCSSGD 432

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +D A  +  EM   G   NVV YT +I    ++ R  +A ++  EM ++GI PD   Y+S
Sbjct: 433 LDGAYNIVKEMIASGCRPNVVIYTTLIKTFLQNSRFGDAMRVLKEMKEQGIAPDIFCYNS 492

Query: 121 LIASLHK 128
           LI  L K
Sbjct: 493 LIIGLSK 499


HSP 4 Score: 80.9 bits (198), Expect = 1.1e-15
Identity = 40/131 (30.53%), Postives = 73/131 (55.73%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  +G      TY   +    K  K+++A ++  EM+ +G+ PD ++Y  LI+G    GN
Sbjct: 583 MVDQGILGDAKTYTVLMNGLFKNDKVDDAEEIFREMRGKGIAPDVFSYGVLINGFSKLGN 642

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + +A  +F+EM + GL  NV+ Y +++ G  + G  ++A +L DEM+ +G+ P+   Y +
Sbjct: 643 MQKASSIFDEMVEEGLTPNVIIYNMLLGGFCRSGEIEKAKELLDEMSVKGLHPNAVTYCT 702

Query: 121 LIASLHKTNPL 132
           +I    K+  L
Sbjct: 703 IIDGYCKSGDL 713


HSP 5 Score: 78.2 bits (191), Expect = 6.9e-15
Identity = 39/122 (31.97%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++G  P+V+ YN  +  +C+ G++E+A +L++EM  +GL P+  TY ++IDG   SG+
Sbjct: 653 MVEEGLTPNVIIYNMLLGGFCRSGEIEKAKELLDEMSVKGLHPNAVTYCTIIDGYCKSGD 712

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  LF+EM   GL  +   YT ++ G  +    + A  ++   NK+G       +++
Sbjct: 713 LAEAFRLFDEMKLKGLVPDSFVYTTLVDGCCRLNDVERAITIFG-TNKKGCASSTAPFNA 772

Query: 121 LI 123
           LI
Sbjct: 773 LI 773


HSP 6 Score: 77.8 bits (190), Expect = 9.0e-15
Identity = 43/127 (33.86%), Postives = 66/127 (51.97%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  P+  TY AFI  Y +  +   A K + EM+E G++P+    T LI+     G 
Sbjct: 513 MVENGLKPNAFTYGAFISGYIEASEFASADKYVKEMRECGVLPNKVLCTGLINEYCKKGK 572

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           V  A   +  M   G+  +  TYTV+++GL K+ + D+A +++ EM  +GI PD   Y  
Sbjct: 573 VIEACSAYRSMVDQGILGDAKTYTVLMNGLFKNDKVDDAEEIFREMRGKGIAPDVFSYGV 632

Query: 121 LIASLHK 128
           LI    K
Sbjct: 633 LINGFSK 639


HSP 7 Score: 75.9 bits (185), Expect = 3.4e-14
Identity = 42/114 (36.84%), Postives = 63/114 (55.26%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P+ VTYN  I+  CK+G +E A +L ++MQ   LMP   TYTSL++G    G       +
Sbjct: 803 PNDVTYNIMIDYLCKEGNLEAAKELFHQMQNANLMPTVITYTSLLNGYDKMGRRAEMFPV 862

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSL 122
           F+E    G+  + + Y+VII+   K+G   +A  L D+M  +  V DDG   S+
Sbjct: 863 FDEAIAAGIEPDHIMYSVIINAFLKEGMTTKALVLVDQMFAKNAV-DDGCKLSI 915


HSP 8 Score: 70.5 bits (171), Expect = 1.4e-12
Identity = 39/122 (31.97%), Postives = 62/122 (50.82%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M   G  P+VV Y   I+ + +  +  +A +++ EM+E+G+ PD + Y SLI G   +  
Sbjct: 443 MIASGCRPNVVIYTTLIKTFLQNSRFGDAMRVLKEMKEQGIAPDIFCYNSLIIGLSKAKR 502

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +D A     EM + GL  N  TY   ISG  +      A K   EM + G++P+  + + 
Sbjct: 503 MDEARSFLVEMVENGLKPNAFTYGAFISGYIEASEFASADKYVKEMRECGVLPNKVLCTG 562

Query: 121 LI 123
           LI
Sbjct: 563 LI 564


HSP 9 Score: 68.9 bits (167), Expect = 4.2e-12
Identity = 42/129 (32.56%), Postives = 65/129 (50.39%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+++G AP +  YN+ I    K  +M+EA   + EM E GL P+ +TY + I G   +  
Sbjct: 478 MKEQGIAPDIFCYNSLIIGLSKAKRMDEARSFLVEMVENGLKPNAFTYGAFISGYIEASE 537

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A +   EM + G+  N V  T +I+   K G+  EA   Y  M  +GI+ D   Y+ 
Sbjct: 538 FASADKYVKEMRECGVLPNKVLCTGLINEYCKKGKVIEACSAYRSMVDQGILGDAKTYTV 597

Query: 121 LIASLHKTN 130
           L+  L K +
Sbjct: 598 LMNGLFKND 606


HSP 10 Score: 67.4 bits (163), Expect = 1.2e-11
Identity = 37/123 (30.08%), Postives = 60/123 (48.78%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+K+    S  TY   ++  C  G ++ AY ++ EM   G  P+   YT+LI     +  
Sbjct: 408 MKKRNIVISPYTYGTVVKGMCSSGDLDGAYNIVKEMIASGCRPNVVIYTTLIKTFLQNSR 467

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A+ +  EM + G+  ++  Y  +I GLSK  R DEA     EM + G+ P+   Y +
Sbjct: 468 FGDAMRVLKEMKEQGIAPDIFCYNSLIIGLSKAKRMDEARSFLVEMVENGLKPNAFTYGA 527

Query: 121 LIA 124
            I+
Sbjct: 528 FIS 530


HSP 11 Score: 60.8 bits (146), Expect = 1.1e-09
Identity = 37/110 (33.64%), Postives = 55/110 (50.00%), Query Frame = 1

Query: 13  YNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMP 72
           Y+  I    K+G ME+A  L + M   GL+P    Y SLI+G     NV +  EL  EM 
Sbjct: 350 YDCCICVMSKEGVMEKAKALFDGMIASGLIPQAQAYASLIEGYCREKNVRQGYELLVEMK 409

Query: 73  QLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
           +  +  +  TY  ++ G+   G  D A+ +  EM   G  P+  IY++LI
Sbjct: 410 KRNIVISPYTYGTVVKGMCSSGDLDGAYNIVKEMIASGCRPNVVIYTTLI 459


HSP 12 Score: 54.3 bits (129), Expect = 1.1e-07
Identity = 33/127 (25.98%), Postives = 59/127 (46.46%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+     P+V+TY + +  Y K G+  E + + +E    G+ PD   Y+ +I+     G 
Sbjct: 831 MQNANLMPTVITYTSLLNGYDKMGRRAEMFPVFDEAIAAGIEPDHIMYSVIINAFLKEGM 890

Query: 61  VDRALELFNEM-----PQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDD 120
             +AL L ++M        G   ++ T   ++SG +K G  + A K+ + M +   +PD 
Sbjct: 891 TTKALVLVDQMFAKNAVDDGCKLSISTCRALLSGFAKVGEMEVAEKVMENMVRLQYIPDS 950

Query: 121 GIYSSLI 123
                LI
Sbjct: 951 ATVIELI 957


HSP 13 Score: 53.1 bits (126), Expect = 2.4e-07
Identity = 37/102 (36.27%), Postives = 51/102 (50.00%), Query Frame = 1

Query: 26  MEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTV 85
           ++ A KL   M  +GL+P  YTY  LIDG      ++ A  L  EM  LG++ +  TY++
Sbjct: 258 VDGALKLKESMICKGLVPLKYTYDVLIDGLCKIKRLEDAKSLLVEMDSLGVSLDNHTYSL 317

Query: 86  IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 128
           +I GL K   AD A  L  EM   GI     +Y   I  + K
Sbjct: 318 LIDGLLKGRNADAAKGLVHEMVSHGINIKPYMYDCCICVMSK 359


HSP 14 Score: 50.4 bits (119), Expect = 1.5e-06
Identity = 39/165 (23.64%), Postives = 68/165 (41.21%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYK-------------------------LINE 60
           M  KG  P+ VTY   I+ YCK G + EA++                          +N+
Sbjct: 688 MSVKGLHPNAVTYCTIIDGYCKSGDLAEAFRLFDEMKLKGLVPDSFVYTTLVDGCCRLND 747

Query: 61  MQ---------ERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR----NVVT 120
           ++         ++G    T  + +LI+     G  +   E+ N +     +R    N VT
Sbjct: 748 VERAITIFGTNKKGCASSTAPFNALINWVFKFGKTELKTEVLNRLMDGSFDRFGKPNDVT 807

Query: 121 YTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 128
           Y ++I  L K+G  + A +L+ +M    ++P    Y+SL+    K
Sbjct: 808 YNIMIDYLCKEGNLEAAKELFHQMQNANLMPTVITYTSLLNGYDK 852

BLAST of Cucsa.306820.2 vs. TAIR10
Match: AT5G65560.1 (AT5G65560.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 104.0 bits (258), Expect = 1.2e-22
Identity = 49/118 (41.53%), Postives = 76/118 (64.41%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P VVTYN+ I+  C+ G  + AY+L++ M +RGL+PD +TYTS+ID    S  V+ A +L
Sbjct: 460 PDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDL 519

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 126
           F+ + Q G+N NVV YT +I G  K G+ DEA  + ++M  +  +P+   +++LI  L
Sbjct: 520 FDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGL 577


HSP 2 Score: 101.3 bits (251), Expect = 7.6e-22
Identity = 54/128 (42.19%), Postives = 79/128 (61.72%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M +KG  P+V+TYNA I  YCK+G +E+A  ++  M+ R L P+T TY  LI G   S N
Sbjct: 384 MLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKS-N 443

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           V +A+ + N+M +  +  +VVTY  +I G  + G  D A++L   MN  G+VPD   Y+S
Sbjct: 444 VHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTS 503

Query: 121 LIASLHKT 129
           +I SL K+
Sbjct: 504 MIDSLCKS 510


HSP 3 Score: 95.9 bits (237), Expect = 3.2e-20
Identity = 51/118 (43.22%), Postives = 70/118 (59.32%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P++ TYN  +  YCK G +EEA + ++++ E GL PD +TYTSLI G     ++D A ++
Sbjct: 216 PNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKV 275

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 126
           FNEMP  G  RN V YT +I GL    R DEA  L+ +M  +   P    Y+ LI SL
Sbjct: 276 FNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSL 333


HSP 4 Score: 91.3 bits (225), Expect = 7.9e-19
Identity = 49/122 (40.16%), Postives = 74/122 (60.66%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME +  +P+  TYN  I+ YCK   + +A  ++N+M ER ++PD  TY SLIDG+  SGN
Sbjct: 419 MESRKLSPNTRTYNELIKGYCKSN-VHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGN 478

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            D A  L + M   GL  +  TYT +I  L K  R +EA  L+D + ++G+ P+  +Y++
Sbjct: 479 FDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTA 538

Query: 121 LI 123
           LI
Sbjct: 539 LI 539


HSP 5 Score: 89.7 bits (221), Expect = 2.3e-18
Identity = 45/122 (36.89%), Postives = 72/122 (59.02%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           +E+KG  P+VV Y A I+ YCK GK++EA+ ++ +M  +  +P++ T+ +LI G  A G 
Sbjct: 523 LEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGK 582

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  L  +M ++GL   V T T++I  L KDG  D A+  + +M   G  PD   Y++
Sbjct: 583 LKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTT 642

Query: 121 LI 123
            I
Sbjct: 643 FI 644


HSP 6 Score: 81.6 bits (200), Expect = 6.2e-16
Identity = 42/122 (34.43%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 8   PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALEL 67
           P+V TY   I+  C   +  EA  L+ EM+E G+ P+ +TYT LID   +    ++A EL
Sbjct: 321 PTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKAREL 380

Query: 68  FNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 127
             +M + GL  NV+TY  +I+G  K G  ++A  + + M    + P+   Y+ LI    K
Sbjct: 381 LGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCK 440

Query: 128 TN 130
           +N
Sbjct: 441 SN 442


HSP 7 Score: 75.9 bits (185), Expect = 3.4e-14
Identity = 43/127 (33.86%), Postives = 67/127 (52.76%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  +G  P   TY + I+  CK  ++EEA  L + ++++G+ P+   YT+LIDG   +G 
Sbjct: 488 MNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGK 547

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD A  +  +M       N +T+  +I GL  DG+  EA  L ++M K G+ P     + 
Sbjct: 548 VDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTI 607

Query: 121 LIASLHK 128
           LI  L K
Sbjct: 608 LIHRLLK 614


HSP 8 Score: 75.5 bits (184), Expect = 4.5e-14
Identity = 44/121 (36.36%), Postives = 62/121 (51.24%), Query Frame = 1

Query: 5   GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 64
           G  P   TY + I  YC++  ++ A+K+ NEM  +G   +   YT LI G   +  +D A
Sbjct: 248 GLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEA 307

Query: 65  LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIAS 124
           ++LF +M        V TYTV+I  L    R  EA  L  EM + GI P+   Y+ LI S
Sbjct: 308 MDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDS 367

Query: 125 L 126
           L
Sbjct: 368 L 368


HSP 9 Score: 75.1 bits (183), Expect = 5.8e-14
Identity = 41/122 (33.61%), Postives = 69/122 (56.56%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME+ G  P++ TY   I+  C + K E+A +L+ +M E+GLMP+  TY +LI+G    G 
Sbjct: 349 MEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGM 408

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A+++   M    L+ N  TY  +I G  K     +A  + ++M +  ++PD   Y+S
Sbjct: 409 IEDAVDVVELMESRKLSPNTRTYNELIKGYCK-SNVHKAMGVLNKMLERKVLPDVVTYNS 468

Query: 121 LI 123
           LI
Sbjct: 469 LI 469


HSP 10 Score: 72.4 bits (176), Expect = 3.8e-13
Identity = 52/153 (33.99%), Postives = 73/153 (47.71%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  K   P+ +T+NA I   C  GK++EA  L  +M + GL P   T T LI      G+
Sbjct: 558 MLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGD 617

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            D A   F +M   G   +  TYT  I    ++GR  +A  +  +M + G+ PD   YSS
Sbjct: 618 FDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAKMRENGVSPDLFTYSS 677

Query: 121 LI---ASLHKTNPLFPYLISRRKLDTFREIHLH 151
           LI     L +TN  F + + +R  DT  E   H
Sbjct: 678 LIKGYGDLGQTN--FAFDVLKRMRDTGCEPSQH 708


HSP 11 Score: 67.4 bits (163), Expect = 1.2e-11
Identity = 48/164 (29.27%), Postives = 77/164 (46.95%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  KG   + V Y   I   C   +++EA  L  +M++    P   TYT LI     S  
Sbjct: 279 MPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSER 338

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              AL L  EM + G+  N+ TYTV+I  L    + ++A +L  +M ++G++P+   Y++
Sbjct: 339 KSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNA 398

Query: 121 LIASLHKTNPL-----FPYLISRRKLD----TFREIHLHFCQKN 156
           LI    K   +        L+  RKL     T+ E+   +C+ N
Sbjct: 399 LINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSN 442


HSP 12 Score: 64.3 bits (155), Expect = 1.0e-10
Identity = 34/113 (30.09%), Postives = 62/113 (54.87%), Query Frame = 1

Query: 13  YNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMP 72
           YN  +    + G ++E  ++  EM E  + P+ YTY  +++G    GNV+ A +  +++ 
Sbjct: 186 YNTLLNSLARFGLVDEMKQVYMEMLEDKVCPNIYTYNKMVNGYCKLGNVEEANQYVSKIV 245

Query: 73  QLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 126
           + GL+ +  TYT +I G  +    D AFK+++EM  +G   ++  Y+ LI  L
Sbjct: 246 EAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGL 298


HSP 13 Score: 60.5 bits (145), Expect = 1.5e-09
Identity = 35/119 (29.41%), Postives = 59/119 (49.58%), Query Frame = 1

Query: 4   KGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 63
           +G +PS + +NA +   CK  K  EA K++++M   G +P   +   LI G    G  +R
Sbjct: 790 EGISPSELVFNALLSCCCKLKKHNEAAKVVDDMICVGHLPQLESCKVLICGLYKKGEKER 849

Query: 64  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
              +F  + Q G   + + + +II G+ K G  +  ++L++ M K G       YS LI
Sbjct: 850 GTSVFQNLLQCGYYEDELAWKIIIDGVGKQGLVEAFYELFNVMEKNGCKFSSQTYSLLI 908

BLAST of Cucsa.306820.2 vs. TAIR10
Match: AT5G39710.1 (AT5G39710.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 103.2 bits (256), Expect = 2.0e-22
Identity = 50/122 (40.98%), Postives = 78/122 (63.93%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M   G +PSVVTYNA I  +C  GKME+A  ++ +M+E+GL PD  +Y++++ G   S +
Sbjct: 406 MNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYD 465

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD AL +  EM + G+  + +TY+ +I G  +  R  EA  LY+EM + G+ PD+  Y++
Sbjct: 466 VDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTA 525

Query: 121 LI 123
           LI
Sbjct: 526 LI 527


HSP 2 Score: 96.3 bits (238), Expect = 2.4e-20
Identity = 50/122 (40.98%), Postives = 79/122 (64.75%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M +KG  P  +TY++ I+ +C++ + +EA  L  EM   GL PD +TYT+LI+     G+
Sbjct: 476 MVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGD 535

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +++AL+L NEM + G+  +VVTY+V+I+GL+K  R  EA +L  ++  E  VP D  Y +
Sbjct: 536 LEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHT 595

Query: 121 LI 123
           LI
Sbjct: 596 LI 597


HSP 3 Score: 95.9 bits (237), Expect = 3.2e-20
Identity = 47/127 (37.01%), Postives = 80/127 (62.99%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M++KG +P VV+Y+  +  +C+   ++EA ++  EM E+G+ PDT TY+SLI G      
Sbjct: 441 MKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRR 500

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A +L+ EM ++GL  +  TYT +I+    +G  ++A +L++EM ++G++PD   YS 
Sbjct: 501 TKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSV 560

Query: 121 LIASLHK 128
           LI  L+K
Sbjct: 561 LINGLNK 567


HSP 4 Score: 94.0 bits (232), Expect = 1.2e-19
Identity = 44/122 (36.07%), Postives = 74/122 (60.66%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  +G  P+  TY   ++ + +KG M EAY+++ EM + G  P   TY +LI+G   +G 
Sbjct: 371 MRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGK 430

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A+ +  +M + GL+ +VV+Y+ ++SG  +    DEA ++  EM ++GI PD   YSS
Sbjct: 431 MEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSS 490

Query: 121 LI 123
           LI
Sbjct: 491 LI 492


HSP 5 Score: 93.2 bits (230), Expect = 2.1e-19
Identity = 48/122 (39.34%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++G +   VTYN  I+ YCK+G   +A  +  EM   GL P   TYTSLI     +GN
Sbjct: 301 MNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGN 360

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++RA+E  ++M   GL  N  TYT ++ G S+ G  +EA+++  EMN  G  P    Y++
Sbjct: 361 MNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNA 420

Query: 121 LI 123
           LI
Sbjct: 421 LI 422


HSP 6 Score: 92.4 bits (228), Expect = 3.5e-19
Identity = 45/141 (31.91%), Postives = 82/141 (58.16%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  PSV+TY + I   CK G M  A + +++M+ RGL P+  TYT+L+DG    G 
Sbjct: 336 MLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGY 395

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A  +  EM   G + +VVTY  +I+G    G+ ++A  + ++M ++G+ PD   YS+
Sbjct: 396 MNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYST 455

Query: 121 LIASLHKTNPLFPYLISRRKL 142
           +++   ++  +   L  +R++
Sbjct: 456 VLSGFCRSYDVDEALRVKREM 476


HSP 7 Score: 89.0 bits (219), Expect = 3.9e-18
Identity = 46/127 (36.22%), Postives = 72/127 (56.69%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME KG  P+VVTYN  I+ YCK  K+++ +KL+  M  +GL P+  +Y  +I+G    G 
Sbjct: 231 METKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLISYNVVINGLCREGR 290

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +     +  EM + G + + VTY  +I G  K+G   +A  ++ EM + G+ P    Y+S
Sbjct: 291 MKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTS 350

Query: 121 LIASLHK 128
           LI S+ K
Sbjct: 351 LIHSMCK 357


HSP 8 Score: 88.2 bits (217), Expect = 6.7e-18
Identity = 43/122 (35.25%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M  KG  P++++YN  I   C++G+M+E   ++ EM  RG   D  TY +LI G    GN
Sbjct: 266 MALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGN 325

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
             +AL +  EM + GL  +V+TYT +I  + K G  + A +  D+M   G+ P++  Y++
Sbjct: 326 FHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTT 385

Query: 121 LI 123
           L+
Sbjct: 386 LV 387


HSP 9 Score: 85.5 bits (210), Expect = 4.3e-17
Identity = 43/135 (31.85%), Postives = 72/135 (53.33%), Query Frame = 1

Query: 7   APSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALE 66
           +P+V TYN  I  +C  G ++ A  L ++M+ +G +P+  TY +LIDG      +D   +
Sbjct: 202 SPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFK 261

Query: 67  LFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 126
           L   M   GL  N+++Y V+I+GL ++GR  E   +  EMN+ G   D+  Y++LI    
Sbjct: 262 LLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYC 321

Query: 127 KTNPLFPYLISRRKL 142
           K       L+   ++
Sbjct: 322 KEGNFHQALVMHAEM 336


HSP 10 Score: 71.6 bits (174), Expect = 6.5e-13
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 5   GKAPSVVTYNAFIERYCK-KGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 64
           G  P V++YNA ++   + K  +  A  +  EM E  + P+ +TY  LI G   +GN+D 
Sbjct: 164 GFMPGVLSYNAVLDATIRSKRNISFAENVFKEMLESQVSPNVFTYNILIRGFCFAGNIDV 223

Query: 65  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 124
           AL LF++M   G   NVVTY  +I G  K  + D+ FKL   M  +G+ P+   Y+ +I 
Sbjct: 224 ALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLISYNVVIN 283

Query: 125 SL 126
            L
Sbjct: 284 GL 285


HSP 11 Score: 62.4 bits (150), Expect = 3.9e-10
Identity = 31/111 (27.93%), Postives = 61/111 (54.95%), Query Frame = 1

Query: 13  YNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGE-RASGNVDRALELFNEM 72
           ++  ++ Y +   +++A  +++  Q  G MP   +Y +++D   R+  N+  A  +F EM
Sbjct: 137 FDLVVKSYSRLSLIDKALSIVHLAQAHGFMPGVLSYNAVLDATIRSKRNISFAENVFKEM 196

Query: 73  PQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
            +  ++ NV TY ++I G    G  D A  L+D+M  +G +P+   Y++LI
Sbjct: 197 LESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLI 247


HSP 12 Score: 59.7 bits (143), Expect = 2.5e-09
Identity = 43/140 (30.71%), Postives = 65/140 (46.43%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M + G  P   TY A I  YC +G +E+A +L NEM E+G++PD  TY+ LI+G      
Sbjct: 511 MLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSR 570

Query: 61  VDRA----LELFNEMPQLGLNRNVVTYTVI--------------ISGLSKDGRADEAFKL 120
              A    L+LF E     +  +V  +T+I              I G    G   EA ++
Sbjct: 571 TREAKRLLLKLFYEE---SVPSDVTYHTLIENCSNIEFKSVVSLIKGFCMKGMMTEADQV 630

Query: 121 YDEMNKEGIVPDDGIYSSLI 123
           ++ M  +   PD   Y+ +I
Sbjct: 631 FESMLGKNHKPDGTAYNIMI 647

BLAST of Cucsa.306820.2 vs. NCBI nr
Match: gi|449440401|ref|XP_004137973.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Cucumis sativus])

HSP 1 Score: 260.0 bits (663), Expect = 3.6e-66
Identity = 129/131 (98.47%), Postives = 129/131 (98.47%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN
Sbjct: 463 MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 522

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS
Sbjct: 523 VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 582

Query: 121 LIASLHKTNPL 132
           LIASLHK  PL
Sbjct: 583 LIASLHKVGPL 593

BLAST of Cucsa.306820.2 vs. NCBI nr
Match: gi|449440401|ref|XP_004137973.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Cucumis sativus])

HSP 1 Score: 86.7 bits (213), Expect = 5.5e-14
Identity = 44/127 (34.65%), Postives = 73/127 (57.48%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME++G AP+VV+++  I+ YCK+    EA +L   M+++G  P   TY + I+     G 
Sbjct: 428 MEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGK 487

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A +L NEM + GL  +  TYT +I G    G  D A +L++EM + G+  +   Y+ 
Sbjct: 488 MEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTV 547

Query: 121 LIASLHK 128
           +I+ L K
Sbjct: 548 IISGLSK 554


HSP 2 Score: 84.0 bits (206), Expect = 3.6e-13
Identity = 46/122 (37.70%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEK     +V TY   IE Y +  K+EEA KL +EM ++G+ PD Y YTS+I+     GN
Sbjct: 253 MEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGN 312

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + RA  LF+EM +  L  N  TY  +I+G  K G    A  + ++M  +G+  +  I+++
Sbjct: 313 MKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNT 372

Query: 121 LI 123
           L+
Sbjct: 373 LM 374


HSP 3 Score: 82.0 bits (201), Expect = 1.4e-12
Identity = 44/122 (36.07%), Postives = 67/122 (54.92%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M++KG      T N     +C+  + EEA +L+  M+ERG+ P+  +++ LID      N
Sbjct: 393 MQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQN 452

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A  LF  M + G   +VVTY   I    K G+ +EA+KL +EM + G++PD   Y+S
Sbjct: 453 FAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTS 512

Query: 121 LI 123
           LI
Sbjct: 513 LI 514


HSP 4 Score: 81.3 bits (199), Expect = 2.3e-12
Identity = 46/124 (37.10%), Postives = 69/124 (55.65%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M KKG  P V  Y + I   CK G M+ A+ L +EM ER L+P+ YTY +LI+G   +G 
Sbjct: 288 MLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGE 347

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  + N+M   G++ N V +  ++ G  K G  DEA +L + M ++G    D    +
Sbjct: 348 MKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGF-EIDAFTCN 407

Query: 121 LIAS 125
           +IAS
Sbjct: 408 IIAS 410


HSP 5 Score: 77.8 bits (190), Expect = 2.6e-11
Identity = 41/113 (36.28%), Postives = 69/113 (61.06%), Query Frame = 1

Query: 10  VVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFN 69
           V ++ A ++  CKKG++  A  L++E+  +G  P   TY +L++G     +V    E+ +
Sbjct: 192 VCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILS 251

Query: 70  EMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
            M +  ++ NV TYT++I   S+  + +EA KL+DEM K+GI PD  IY+S+I
Sbjct: 252 LMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSII 304


HSP 6 Score: 76.6 bits (187), Expect = 5.7e-11
Identity = 41/122 (33.61%), Postives = 65/122 (53.28%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++   P+  TY A I   CK G+M+ A  ++N+MQ +G+  +   + +L+DG    G 
Sbjct: 323 MTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGM 382

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +D AL L N M Q G   +  T  +I SG  +  R +EA +L   M + G+ P+   +S 
Sbjct: 383 IDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSI 442

Query: 121 LI 123
           LI
Sbjct: 443 LI 444


HSP 7 Score: 70.5 bits (171), Expect = 4.1e-09
Identity = 40/119 (33.61%), Postives = 66/119 (55.46%), Query Frame = 1

Query: 4   KGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 63
           KG  PSV+TYN  +  Y +   +    ++++ M++  +  +  TYT LI+    S  ++ 
Sbjct: 221 KGFKPSVITYNTLLNGYIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEE 280

Query: 64  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
           A +LF+EM + G+  +V  YT II+   K G    AF L+DEM +  +VP+   Y +LI
Sbjct: 281 AEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALI 339


HSP 8 Score: 70.1 bits (170), Expect = 5.3e-09
Identity = 39/122 (31.97%), Postives = 65/122 (53.28%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ KG   + V +N  ++ YCKKG ++EA +L N MQ++G   D +T   +  G   S  
Sbjct: 358 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 417

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            + A  L   M + G+  NVV+++++I    K+    EA +L+  M K+G  P    Y++
Sbjct: 418 REEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNA 477

Query: 121 LI 123
            I
Sbjct: 478 FI 479


HSP 9 Score: 218.4 bits (555), Expect = 1.2e-53
Identity = 107/127 (84.25%), Postives = 118/127 (92.91%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEKKG+ PSVVTYN FI+RYCKKGKMEEAYKLI+EM+ERGLMPDTYTYTSL++ E ASGN
Sbjct: 463 MEKKGEVPSVVTYNTFIQRYCKKGKMEEAYKLIDEMKERGLMPDTYTYTSLMNWECASGN 522

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VDRALELFNEMPQ GLNRN VTYTV+ISGLSK GRADEAFKLYDEMN +GI+PDD I+SS
Sbjct: 523 VDRALELFNEMPQRGLNRNEVTYTVMISGLSKAGRADEAFKLYDEMNTQGIIPDDRIFSS 582

Query: 121 LIASLHK 128
           LIASLH+
Sbjct: 583 LIASLHE 589

BLAST of Cucsa.306820.2 vs. NCBI nr
Match: gi|659084068|ref|XP_008442691.1| (PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At2g32630-like [Cucumis melo])

HSP 1 Score: 85.5 bits (210), Expect = 1.2e-13
Identity = 46/122 (37.70%), Postives = 72/122 (59.02%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEK     +V TY   IE Y +  K+EE+ KL +EM ++G+ PD Y YTSLI+     GN
Sbjct: 253 MEKDVVDYNVATYTMLIEWYSRISKIEESEKLFDEMLKKGIEPDVYVYTSLINWNCKFGN 312

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + RA  LF+EM +  L  N  TY  +++G  K G+ + A  + ++M  +G+  +  I+++
Sbjct: 313 MKRAFVLFDEMTERRLVPNAFTYGALVNGACKAGQMEAAEMMVNDMQSKGVDVNQVIFNT 372

Query: 121 LI 123
           LI
Sbjct: 373 LI 374


HSP 2 Score: 85.1 bits (209), Expect = 1.6e-13
Identity = 46/122 (37.70%), Postives = 68/122 (55.74%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M++KG    V T N     +C+  + EEA +L+  M+ERG+ PD  +++ LID      N
Sbjct: 393 MQQKGFEIDVFTCNIIASGFCRLDRREEARRLLLTMEERGVAPDVVSFSILIDIYGKEEN 452

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A  L  EM + G   +VVTY   I    K G+ +EA+KL DEM + G++PD   Y+S
Sbjct: 453 FAEAKRLLKEMEKKGEVPSVVTYNTFIQRYCKKGKMEEAYKLIDEMKERGLMPDTYTYTS 512

Query: 121 LI 123
           L+
Sbjct: 513 LM 514


HSP 3 Score: 82.0 bits (201), Expect = 1.4e-12
Identity = 41/127 (32.28%), Postives = 74/127 (58.27%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME++G AP VV+++  I+ Y K+    EA +L+ EM+++G +P   TY + I      G 
Sbjct: 428 MEERGVAPDVVSFSILIDIYGKEENFAEAKRLLKEMEKKGEVPSVVTYNTFIQRYCKKGK 487

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           ++ A +L +EM + GL  +  TYT +++     G  D A +L++EM + G+  ++  Y+ 
Sbjct: 488 MEEAYKLIDEMKERGLMPDTYTYTSLMNWECASGNVDRALELFNEMPQRGLNRNEVTYTV 547

Query: 121 LIASLHK 128
           +I+ L K
Sbjct: 548 MISGLSK 554


HSP 4 Score: 80.5 bits (197), Expect = 3.9e-12
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++   P+  TY A +   CK G+ME A  ++N+MQ +G+  +   + +LIDG    G 
Sbjct: 323 MTERRLVPNAFTYGALVNGACKAGQMEAAEMMVNDMQSKGVDVNQVIFNTLIDGYCXKGM 382

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +D AL L N M Q G   +V T  +I SG  +  R +EA +L   M + G+ PD   +S 
Sbjct: 383 IDEALRLQNIMQQKGFEIDVFTCNIIASGFCRLDRREEARRLLLTMEERGVAPDVVSFSI 442

Query: 121 LI 123
           LI
Sbjct: 443 LI 444


HSP 5 Score: 79.7 bits (195), Expect = 6.7e-12
Identity = 41/114 (35.96%), Postives = 64/114 (56.14%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M KKG  P V  Y + I   CK G M+ A+ L +EM ER L+P+ +TY +L++G   +G 
Sbjct: 288 MLKKGIEPDVYVYTSLINWNCKFGNMKRAFVLFDEMTERRLVPNAFTYGALVNGACKAGQ 347

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPD 115
           ++ A  + N+M   G++ N V +  +I G    G  DEA +L + M ++G   D
Sbjct: 348 MEAAEMMVNDMQSKGVDVNQVIFNTLIDGYCXKGMIDEALRLQNIMQQKGFEID 401


HSP 6 Score: 73.9 bits (180), Expect = 3.7e-10
Identity = 39/113 (34.51%), Postives = 69/113 (61.06%), Query Frame = 1

Query: 10  VVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFN 69
           V ++ A ++  CKKG++  A  L++E+  +G  P+  TY +L++G     +     E+ +
Sbjct: 192 VSSWTAVVDGLCKKGEVVRAKALVDELVCKGFKPNVITYNTLLNGYIEIKDEGGVNEILS 251

Query: 70  EMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
            M +  ++ NV TYT++I   S+  + +E+ KL+DEM K+GI PD  +Y+SLI
Sbjct: 252 LMEKDVVDYNVATYTMLIEWYSRISKIEESEKLFDEMLKKGIEPDVYVYTSLI 304


HSP 7 Score: 178.7 bits (452), Expect = 1.1e-41
Identity = 91/131 (69.47%), Postives = 104/131 (79.39%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M++KG      T N     +C+  + EEA +L+  M+ERG+ P+  ++    +GERASGN
Sbjct: 393 MQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSF----NGERASGN 452

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS
Sbjct: 453 VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 512

Query: 121 LIASLHKTNPL 132
           LIASLHK  PL
Sbjct: 513 LIASLHKVGPL 519

BLAST of Cucsa.306820.2 vs. NCBI nr
Match: gi|700203836|gb|KGN58969.1| (hypothetical protein Csa_3G740130 [Cucumis sativus])

HSP 1 Score: 84.0 bits (206), Expect = 3.6e-13
Identity = 46/122 (37.70%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEK     +V TY   IE Y +  K+EEA KL +EM ++G+ PD Y YTS+I+     GN
Sbjct: 253 MEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGN 312

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + RA  LF+EM +  L  N  TY  +I+G  K G    A  + ++M  +G+  +  I+++
Sbjct: 313 MKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNT 372

Query: 121 LI 123
           L+
Sbjct: 373 LM 374


HSP 2 Score: 81.3 bits (199), Expect = 2.3e-12
Identity = 46/124 (37.10%), Postives = 69/124 (55.65%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M KKG  P V  Y + I   CK G M+ A+ L +EM ER L+P+ YTY +LI+G   +G 
Sbjct: 288 MLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGE 347

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  + N+M   G++ N V +  ++ G  K G  DEA +L + M ++G    D    +
Sbjct: 348 MKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGF-EIDAFTCN 407

Query: 121 LIAS 125
           +IAS
Sbjct: 408 IIAS 410


HSP 3 Score: 77.8 bits (190), Expect = 2.6e-11
Identity = 41/113 (36.28%), Postives = 69/113 (61.06%), Query Frame = 1

Query: 10  VVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFN 69
           V ++ A ++  CKKG++  A  L++E+  +G  P   TY +L++G     +V    E+ +
Sbjct: 192 VCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILS 251

Query: 70  EMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
            M +  ++ NV TYT++I   S+  + +EA KL+DEM K+GI PD  IY+S+I
Sbjct: 252 LMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSII 304


HSP 4 Score: 74.7 bits (182), Expect = 2.2e-10
Identity = 38/114 (33.33%), Postives = 61/114 (53.51%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M ++   P+  TY A I   CK G+M+ A  ++N+MQ +G+  +   + +L+DG    G 
Sbjct: 323 MTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGM 382

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPD 115
           +D AL L N M Q G   +  T  +I SG  +  R +EA +L   M + G+ P+
Sbjct: 383 IDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPN 436


HSP 5 Score: 70.5 bits (171), Expect = 4.1e-09
Identity = 40/119 (33.61%), Postives = 66/119 (55.46%), Query Frame = 1

Query: 4   KGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDR 63
           KG  PSV+TYN  +  Y +   +    ++++ M++  +  +  TYT LI+    S  ++ 
Sbjct: 221 KGFKPSVITYNTLLNGYIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEE 280

Query: 64  ALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 123
           A +LF+EM + G+  +V  YT II+   K G    AF L+DEM +  +VP+   Y +LI
Sbjct: 281 AEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALI 339


HSP 6 Score: 67.0 bits (162), Expect = 4.5e-08
Identity = 40/127 (31.50%), Postives = 67/127 (52.76%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ KG   + V +N  ++ YCKKG ++EA +L N MQ++G   D +T   +  G   S  
Sbjct: 358 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 417

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            + A  L   M + G+  NVV++    +G    G  D A +L++EM + G+  +   Y+ 
Sbjct: 418 REEAKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTV 477

Query: 121 LIASLHK 128
           +I+ L K
Sbjct: 478 IISGLSK 480


HSP 7 Score: 177.9 bits (450), Expect = 1.8e-41
Identity = 85/126 (67.46%), Postives = 102/126 (80.95%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME+KG+ P+ VTYNA I+ Y KKGKM+EA+KL  EM+++GLMPDTYTY+SLI GE   G 
Sbjct: 377 MERKGERPNTVTYNALIDGYIKKGKMKEAHKLKKEMEDKGLMPDTYTYSSLIHGECIDGK 436

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD AL+LF+EM Q  L RNVVTYT +ISGLSKDGR DEAFKLYDEM +EG+ PDD +Y S
Sbjct: 437 VDEALKLFHEMYQRNLTRNVVTYTAMISGLSKDGRTDEAFKLYDEMKREGLTPDDRVYHS 496

Query: 121 LIASLH 127
           L+ SLH
Sbjct: 497 LVGSLH 502

BLAST of Cucsa.306820.2 vs. NCBI nr
Match: gi|595838612|ref|XP_007207685.1| (hypothetical protein PRUPE_ppa024892mg [Prunus persica])

HSP 1 Score: 100.1 bits (248), Expect = 4.8e-18
Identity = 57/161 (35.40%), Postives = 81/161 (50.31%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME++G AP+VV +   I+ +CK+G   EA ++I EM+ +G  P+T TY +LIDG    G 
Sbjct: 342 MEERGVAPNVVCFTTLIDIFCKEGNFVEAKQVIQEMERKGERPNTVTYNALIDGYIKKGK 401

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLY----------------- 120
           +  A +L  EM   GL  +  TY+ +I G   DG+ DEA KL+                 
Sbjct: 402 MKEAHKLKKEMEDKGLMPDTYTYSSLIHGECIDGKVDEALKLFHEMYQRNLTRNVVTYTA 461

Query: 121 ------------------DEMNKEGIVPDDGIYSSLIASLH 127
                             DEM +EG+ PDD +Y SL+ SLH
Sbjct: 462 MISGLSKDGRTDEAFKLYDEMKREGLTPDDRVYHSLVGSLH 502


HSP 2 Score: 89.4 bits (220), Expect = 8.5e-15
Identity = 50/122 (40.98%), Postives = 66/122 (54.10%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           ME KG    V TY+      CK  + EEA  L+  M+ERG+ P+   +T+LID     GN
Sbjct: 307 MEGKGFQTDVFTYSTIASGLCKLNRNEEAKSLLFTMEERGVAPNVVCFTTLIDIFCKEGN 366

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
              A ++  EM + G   N VTY  +I G  K G+  EA KL  EM  +G++PD   YSS
Sbjct: 367 FVEAKQVIQEMERKGERPNTVTYNALIDGYIKKGKMKEAHKLKKEMEDKGLMPDTYTYSS 426

Query: 121 LI 123
           LI
Sbjct: 427 LI 428


HSP 3 Score: 82.4 bits (202), Expect = 1.0e-12
Identity = 47/122 (38.52%), Postives = 70/122 (57.38%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEK     +  T    I+ +    K+E+A K+  E+ ERG+ PD Y YTS+I+     GN
Sbjct: 167 MEKDSVGYNAATCTLLIDWFGSCEKIEDAEKVFEEIHERGIEPDVYLYTSIINWNGRVGN 226

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           + RAL LF+E+ Q GL  NV TY  +I+G  K G+   A  L +EM  +GI  +  ++++
Sbjct: 227 MKRALFLFDELTQRGLVPNVHTYGALINGACKGGQMKMADILVNEMQSKGIDVNQVVFNT 286

Query: 121 LI 123
           LI
Sbjct: 287 LI 288


HSP 4 Score: 81.6 bits (200), Expect = 1.8e-12
Identity = 46/122 (37.70%), Postives = 69/122 (56.56%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           + ++G  P+V TY A I   CK G+M+ A  L+NEMQ +G+  +   + +LIDG    G 
Sbjct: 237 LTQRGLVPNVHTYGALINGACKGGQMKMADILVNEMQSKGIDVNQVVFNTLIDGYCRKGM 296

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +D AL L + M   G   +V TY+ I SGL K  R +EA  L   M + G+ P+   +++
Sbjct: 297 MDEALRLQDFMEGKGFQTDVFTYSTIASGLCKLNRNEEAKSLLFTMEERGVAPNVVCFTT 356

Query: 121 LI 123
           LI
Sbjct: 357 LI 358


HSP 5 Score: 79.7 bits (195), Expect = 6.7e-12
Identity = 44/129 (34.11%), Postives = 69/129 (53.49%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           + ++G  P V  Y + I    + G M+ A  L +E+ +RGL+P+ +TY +LI+G    G 
Sbjct: 202 IHERGIEPDVYLYTSIINWNGRVGNMKRALFLFDELTQRGLVPNVHTYGALINGACKGGQ 261

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           +  A  L NEM   G++ N V +  +I G  + G  DEA +L D M  +G   D   YS+
Sbjct: 262 MKMADILVNEMQSKGIDVNQVVFNTLIDGYCRKGMMDEALRLQDFMEGKGFQTDVFTYST 321

Query: 121 LIASLHKTN 130
           + + L K N
Sbjct: 322 IASGLCKLN 330


HSP 6 Score: 74.7 bits (182), Expect = 2.2e-10
Identity = 40/122 (32.79%), Postives = 67/122 (54.92%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M+ KG   + V +N  I+ YC+KG M+EA +L + M+ +G   D +TY+++  G      
Sbjct: 272 MQSKGIDVNQVVFNTLIDGYCRKGMMDEALRLQDFMEGKGFQTDVFTYSTIASGLCKLNR 331

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            + A  L   M + G+  NVV +T +I    K+G   EA ++  EM ++G  P+   Y++
Sbjct: 332 NEEAKSLLFTMEERGVAPNVVCFTTLIDIFCKEGNFVEAKQVIQEMERKGERPNTVTYNA 391

Query: 121 LI 123
           LI
Sbjct: 392 LI 393


HSP 7 Score: 58.5 bits (140), Expect = 1.6e-05
Identity = 32/122 (26.23%), Postives = 65/122 (53.28%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           M +KG   +V +    +   CK+G++++A  L+ EM  R + P+  TY +L+       +
Sbjct: 97  MVEKGVQITVHSLTLVMNELCKRGEVKKAKALMGEMAGRQVKPNVVTYNTLLKAYIERKD 156

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
            +   E  + M +  +  N  T T++I       + ++A K+++E+++ GI PD  +Y+S
Sbjct: 157 FEGVNEALSLMEKDSVGYNAATCTLLIDWFGSCEKIEDAEKVFEEIHERGIEPDVYLYTS 216

Query: 121 LI 123
           +I
Sbjct: 217 II 218


HSP 8 Score: 177.6 bits (449), Expect = 2.4e-41
Identity = 82/130 (63.08%), Postives = 106/130 (81.54%), Query Frame = 1

Query: 1   MEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGN 60
           MEKKG+ P++VTYN  I+ Y K+ KM+EAYKL +EM+++G+MPD+YTYTSL+ GE  +  
Sbjct: 490 MEKKGEKPNIVTYNTLIDGYIKQRKMKEAYKLKDEMEDKGIMPDSYTYTSLVHGECVAEK 549

Query: 61  VDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSS 120
           VD AL++FNEMP+ GL RNVVTYT +I GLSK+GRADEAF LYD+M K G+ PDD +YSS
Sbjct: 550 VDEALKMFNEMPRRGLTRNVVTYTAMILGLSKEGRADEAFNLYDDMIKAGLTPDDRVYSS 609

Query: 121 LIASLHKTNP 131
           L++SLH T P
Sbjct: 610 LVSSLHTTKP 619

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP180_ARATH4.0e-3354.76Pentatricopeptide repeat-containing protein At2g32630 OS=Arabidopsis thaliana GN... [more]
PP143_ARATH5.5e-2242.62Putative pentatricopeptide repeat-containing protein At2g02150 OS=Arabidopsis th... [more]
PP442_ARATH1.2e-2141.09Pentatricopeptide repeat-containing protein At5g61990, mitochondrial OS=Arabidop... [more]
PP445_ARATH2.1e-2141.53Pentatricopeptide repeat-containing protein At5g65560 OS=Arabidopsis thaliana GN... [more]
PP407_ARATH3.6e-2140.98Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana GN... [more]
Match NameE-valueIdentityDescription
A0A0A0LDI0_CUCSA7.4e-4269.47Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1[more]
A0A0A0LDI0_CUCSA2.5e-1337.70Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1[more]
M5W590_PRUPE3.4e-1835.40Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024892mg PE=4 SV=1[more]
F6HR46_VITVI1.7e-1738.58Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0040g01530 PE=4 SV=... [more]
G7I783_MEDTR3.7e-1740.16PPR containing plant-like protein OS=Medicago truncatula GN=MTR_1g045880 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT2G32630.12.3e-3454.76 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT2G02150.13.1e-2342.62 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT5G61990.16.9e-2341.09 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT5G65560.11.2e-2241.53 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT5G39710.12.0e-2240.98 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449440401|ref|XP_004137973.1|3.6e-6698.47PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Cucumis sativu... [more]
gi|449440401|ref|XP_004137973.1|5.5e-1434.65PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Cucumis sativu... [more]
gi|659084068|ref|XP_008442691.1|1.2e-1337.70PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At2g... [more]
gi|700203836|gb|KGN58969.1|3.6e-1337.70hypothetical protein Csa_3G740130 [Cucumis sativus][more]
gi|595838612|ref|XP_007207685.1|4.8e-1835.40hypothetical protein PRUPE_ppa024892mg [Prunus persica][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.306820Cucsa.306820gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.306820.2Cucsa.306820.2-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.306820.2.CDS.1Cucsa.306820.2.CDS.1CDS
Cucsa.306820.2.CDS.2Cucsa.306820.2.CDS.2CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.306820.2.three_prime_UTR.1Cucsa.306820.2.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 79..126
score: 5.2E-15coord: 8..54
score: 3.3
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 81..114
score: 5.8E-11coord: 46..79
score: 1.1E-4coord: 11..45
score: 6.4
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 9..43
score: 14.063coord: 44..78
score: 10.83coord: 79..113
score: 13
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 1..125
score: 4.4