Cucsa.306820.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.306820.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPentatricopeptide repeat-containing protein family
Locationscaffold02977 : 234484 .. 236250 (+)
Sequence length1767
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAAGCAGGCGATTGCCGCGAACATTGCGAAGCTAATTCTGAAATCTGGCCTTCAACCCTTCAAAACCACCCCATCGCTGCTTTCAAATTTTGATTCGCGGGTAATGCAATTGGTTTTATCTGATCCAAATCTTCCTACTCGGTCCTGTTTGCGGTTTTTCGACTTTCTCCGACAAAACCCATCTCGTAAACCCGATCTTCCGGCGCATTTAATCCTCTTCTCTAGGTTGTATCGAGCTCGGAAGTTCGCGGAAATGAAAAATGTGTTGAAATTCATCGTTAATGATGGAAATCTTTGGAGTAATGTTGAGCGGATTGTTtCTTCGATTGGAGGTGAGTTTAATGAGCCGAGCATCGTTGAAAAATTTTGTGATATGTTGTTTAGAGTATACATGGATAACAGAATGTTTGATTCGTCTTTGGAGGTTTTTGATTATGCGAGAAAGAAAGGGTTTGAGATTGACGAGAGATCATGTTTTGAGTTTCTACTTGCTTTGAAGAGATCTGGTAATATGGAATTATGTGTAGAATTCTTGCGTCAAATGGTCGATTCGGGCATAGAAATACGTGTTTGTTCGTGGACGGCTGTGGTTGATGGGTTGTGTAAGAAAGGGGAGGTTGTAAGGGCTAAAGCTTTGATGGATGAACTTGTCTGTAAAGGATTTAAGCCCAGTGTTATAACATATAATACTCTTTTGAATGGTTATATTGAAATTAAGGATGTGGGAGGTGTTAATGAGATTCTTAGTTTGATGGAGAAGAATGTTGTGGATTATAATGTAACAACATATACAATGTTGATTGAATGGTATTCAAGAAGTTCGAAAATTGAGGAAGCAGAGAAGCTGTTTGATGAAATGCTTAAGAAAGGAATAGAGCCTGATGTGTATATTTACACCTCCATTATCAATTGGAATTGTAAATTTGGGAACATGAAGAGGGCCTTTGTTCTGTTTGATGAAATGACTGAGAGAAGGCTTGTTCCAAATGCATACACTTATGGTGCCCTTATAAATGGTGCCTGCAAGGCAGGGGAGATGAAGGCAGCTGAGATGATGGTAAATGACATGCAAAGCAAAGGGGTTGATGTAAATCGAGTGATATTCAATACATTGATGGATGGGTACTGCAAAAAaGGAaTGATTGACGAAGCTCTAAGGCTGCAGAATATCATGCAGCAAAAAGGATTTGAGATTGATGCATTTACTTGTAACATAATTGCCAGTGGATTTTGTAGATCGAATCGGCGAGAGGAAGCAAAGAGACTATTGCTTACAATGGAAGAGAGAGGAGTGGCTCCAAATGTAGTGAGCTTCAGTATTTTGATTGACATTTACTGCAAAGAACAAAATTTTGCAGAAGCAAGAAGGCTGTTCAAGGTAATGGAGAAGAAGGGGAAGGCACCTAGTGTTGTTACGTACAATGCTTTCATTGAAAGATATTGCAAGAAGGGAAAGATGGAGGAGGCATACAAACTGATAAATGAGATGCAAGAAAGGGGGTTAATGCCGGACACATATACATATACATCACTTATAGATGGGGAACGTGCTAGTGGGAATGTGGATAGAGCGCTTGAACTATTCAATGAAATGCCACAACTAGGACTAAATCGAAATGTAGTAACTTACACGGTAATAATCTCTGGGTTGTCCAAGGATGGTAGAGCTGATGAAGCTTTTAAATTATATGATGAAATGAACAAAGAAGGCATTGTACCTGATGACGGAATATATTCTTCCTTGATAGCGAGCCTTCATAAG

mRNA sequence

ATGTCGAAGCAGGCGATTGCCGCGAACATTGCGAAGCTAATTCTGAAATCTGGCCTTCAACCCTTCAAAACCACCCCATCGCTGCTTTCAAATTTTGATTCGCGGGTAATGCAATTGGTTTTATCTGATCCAAATCTTCCTACTCGGTCCTGTTTGCGGTTTTTCGACTTTCTCCGACAAAACCCATCTCGTAAACCCGATCTTCCGGCGCATTTAATCCTCTTCTCTAGGTTGTATCGAGCTCGGAAGTTCGCGGAAATGAAAAATGTGTTGAAATTCATCGTTAATGATGGAAATCTTTGGAGTAATGTTGAGCGGATTGTTTCTTCGATTGGAGGTGAGTTTAATGAGCCGAGCATCGTTGAAAAATTTTGTGATATGTTGTTTAGAGTATACATGGATAACAGAATGTTTGATTCGTCTTTGGAGGTTTTTGATTATGCGAGAAAGAAAGGGTTTGAGATTGACGAGAGATCATGTTTTGAGTTTCTACTTGCTTTGAAGAGATCTGGTAATATGGAATTATGTGTAGAATTCTTGCGTCAAATGGTCGATTCGGGCATAGAAATACGTGTTTGTTCGTGGACGGCTGTGGTTGATGGGTTGTGTAAGAAAGGGGAGGTTGTAAGGGCTAAAGCTTTGATGGATGAACTTGTCTGTAAAGGATTTAAGCCCAGTGTTATAACATATAATACTCTTTTGAATGGTTATATTGAAATTAAGGATGTGGGAGGTGTTAATGAGATTCTTAGTTTGATGGAGAAGAATGTTGTGGATTATAATGTAACAACATATACAATGTTGATTGAATGGTATTCAAGAAGTTCGAAAATTGAGGAAGCAGAGAAGCTGTTTGATGAAATGCTTAAGAAAGGAATAGAGCCTGATGTGTATATTTACACCTCCATTATCAATTGGAATTGTAAATTTGGGAACATGAAGAGGGCCTTTGTTCTGTTTGATGAAATGACTGAGAGAAGGCTTGTTCCAAATGCATACACTTATGGTGCCCTTATAAATGGTGCCTGCAAGGCAGGGGAGATGAAGGCAGCTGAGATGATGGTAAATGACATGCAAAGCAAAGGGGTTGATGTAAATCGAGTGATATTCAATACATTGATGGATGGGTACTGCAAAAAAGGAATGATTGACGAAGCTCTAAGGCTGCAGAATATCATGCAGCAAAAAGGATTTGAGATTGATGCATTTACTTGTAACATAATTGCCAGTGGATTTTGTAGATCGAATCGGCGAGAGGAAGCAAAGAGACTATTGCTTACAATGGAAGAGAGAGGAGTGGCTCCAAATGTAGTGAGCTTCAGTATTTTGATTGACATTTACTGCAAAGAACAAAATTTTGCAGAAGCAAGAAGGCTGTTCAAGGTAATGGAGAAGAAGGGGAAGGCACCTAGTGTTGTTACGTACAATGCTTTCATTGAAAGATATTGCAAGAAGGGAAAGATGGAGGAGGCATACAAACTGATAAATGAGATGCAAGAAAGGGGGTTAATGCCGGACACATATACATATACATCACTTATAGATGGGGAACGTGCTAGTGGGAATGTGGATAGAGCGCTTGAACTATTCAATGAAATGCCACAACTAGGACTAAATCGAAATGTAGTAACTTACACGGTAATAATCTCTGGGTTGTCCAAGGATGGTAGAGCTGATGAAGCTTTTAAATTATATGATGAAATGAACAAAGAAGGCATTGTACCTGATGACGGAATATATTCTTCCTTGATAGCGAGCCTTCATAAG

Coding sequence (CDS)

ATGTCGAAGCAGGCGATTGCCGCGAACATTGCGAAGCTAATTCTGAAATCTGGCCTTCAACCCTTCAAAACCACCCCATCGCTGCTTTCAAATTTTGATTCGCGGGTAATGCAATTGGTTTTATCTGATCCAAATCTTCCTACTCGGTCCTGTTTGCGGTTTTTCGACTTTCTCCGACAAAACCCATCTCGTAAACCCGATCTTCCGGCGCATTTAATCCTCTTCTCTAGGTTGTATCGAGCTCGGAAGTTCGCGGAAATGAAAAATGTGTTGAAATTCATCGTTAATGATGGAAATCTTTGGAGTAATGTTGAGCGGATTGTTtCTTCGATTGGAGGTGAGTTTAATGAGCCGAGCATCGTTGAAAAATTTTGTGATATGTTGTTTAGAGTATACATGGATAACAGAATGTTTGATTCGTCTTTGGAGGTTTTTGATTATGCGAGAAAGAAAGGGTTTGAGATTGACGAGAGATCATGTTTTGAGTTTCTACTTGCTTTGAAGAGATCTGGTAATATGGAATTATGTGTAGAATTCTTGCGTCAAATGGTCGATTCGGGCATAGAAATACGTGTTTGTTCGTGGACGGCTGTGGTTGATGGGTTGTGTAAGAAAGGGGAGGTTGTAAGGGCTAAAGCTTTGATGGATGAACTTGTCTGTAAAGGATTTAAGCCCAGTGTTATAACATATAATACTCTTTTGAATGGTTATATTGAAATTAAGGATGTGGGAGGTGTTAATGAGATTCTTAGTTTGATGGAGAAGAATGTTGTGGATTATAATGTAACAACATATACAATGTTGATTGAATGGTATTCAAGAAGTTCGAAAATTGAGGAAGCAGAGAAGCTGTTTGATGAAATGCTTAAGAAAGGAATAGAGCCTGATGTGTATATTTACACCTCCATTATCAATTGGAATTGTAAATTTGGGAACATGAAGAGGGCCTTTGTTCTGTTTGATGAAATGACTGAGAGAAGGCTTGTTCCAAATGCATACACTTATGGTGCCCTTATAAATGGTGCCTGCAAGGCAGGGGAGATGAAGGCAGCTGAGATGATGGTAAATGACATGCAAAGCAAAGGGGTTGATGTAAATCGAGTGATATTCAATACATTGATGGATGGGTACTGCAAAAAaGGAaTGATTGACGAAGCTCTAAGGCTGCAGAATATCATGCAGCAAAAAGGATTTGAGATTGATGCATTTACTTGTAACATAATTGCCAGTGGATTTTGTAGATCGAATCGGCGAGAGGAAGCAAAGAGACTATTGCTTACAATGGAAGAGAGAGGAGTGGCTCCAAATGTAGTGAGCTTCAGTATTTTGATTGACATTTACTGCAAAGAACAAAATTTTGCAGAAGCAAGAAGGCTGTTCAAGGTAATGGAGAAGAAGGGGAAGGCACCTAGTGTTGTTACGTACAATGCTTTCATTGAAAGATATTGCAAGAAGGGAAAGATGGAGGAGGCATACAAACTGATAAATGAGATGCAAGAAAGGGGGTTAATGCCGGACACATATACATATACATCACTTATAGATGGGGAACGTGCTAGTGGGAATGTGGATAGAGCGCTTGAACTATTCAATGAAATGCCACAACTAGGACTAAATCGAAATGTAGTAACTTACACGGTAATAATCTCTGGGTTGTCCAAGGATGGTAGAGCTGATGAAGCTTTTAAATTATATGATGAAATGAACAAAGAAGGCATTGTACCTGATGACGGAATATATTCTTCCTTGATAGCGAGCCTTCATAAG

Protein sequence

MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK
BLAST of Cucsa.306820.1 vs. Swiss-Prot
Match: PP180_ARATH (Pentatricopeptide repeat-containing protein At2g32630 OS=Arabidopsis thaliana GN=At2g32630 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 5.0e-174
Identity = 312/592 (52.70%), Postives = 425/592 (71.79%), Query Frame = 1

Query: 2   SKQAIAANI-AKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 61
           S Q  A  I A L+ KS +   ++ PSLL N +S V +LVLS+P LPT+SC+ FF  LR+
Sbjct: 29  SDQEAARRITACLVEKSTIGKLQSNPSLLFNLNSNVTRLVLSEPTLPTQSCIDFFKLLRE 88

Query: 62  NPSR-KPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSS-IGGEFNEP 121
             S  KPDL A + L  RLY  R+F EM+++L  +VNDG     VE + S+ +  + +E 
Sbjct: 89  FESNLKPDLTAVVTLSHRLYSNRRFNEMRSLLNSVVNDGFYKRPVEELGSAMVDCDISEE 148

Query: 122 SI--VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELC 181
                EKF D++FRVY+DN MF+  L VFDY  KKG  IDERSC  FL+A K+   ++LC
Sbjct: 149 KFEFFEKFFDLVFRVYVDNGMFEEGLRVFDYMVKKGLSIDERSCIVFLVAAKKRRRIDLC 208

Query: 182 VEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNG 241
           +E  R+MVDSG++I V S T VV+GLC++GEV ++K L+ E   KG KP   TYNT++N 
Sbjct: 209 LEIFRRMVDSGVKITVYSLTIVVEGLCRRGEVEKSKKLIKEFSVKGIKPEAYTYNTIINA 268

Query: 242 YIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPD 301
           Y++ +D  GV  +L +M+K+ V YN  TYT+L+E   ++ K+ +AEKLFDEM ++GIE D
Sbjct: 269 YVKQRDFSGVEGVLKVMKKDGVVYNKVTYTLLMELSVKNGKMSDAEKLFDEMRERGIESD 328

Query: 302 VYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVN 361
           V++YTS+I+WNC+ GNMKRAF+LFDE+TE+ L P++YTYGALI+G CK GEM AAE+++N
Sbjct: 329 VHVYTSLISWNCRKGNMKRAFLLFDELTEKGLSPSSYTYGALIDGVCKVGEMGAAEILMN 388

Query: 362 DMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSN 421
           +MQSKGV++ +V+FNTL+DGYC+KGM+DEA  + ++M+QKGF+ D FTCN IAS F R  
Sbjct: 389 EMQSKGVNITQVVFNTLIDGYCRKGMVDEASMIYDVMEQKGFQADVFTCNTIASCFNRLK 448

Query: 422 RREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYN 481
           R +EAK+ L  M E GV  + VS++ LID+YCKE N  EA+RLF  M  KG  P+ +TYN
Sbjct: 449 RYDEAKQWLFRMMEGGVKLSTVSYTNLIDVYCKEGNVEEAKRLFVEMSSKGVQPNAITYN 508

Query: 482 AFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQL 541
             I  YCK+GK++EA KL   M+  G+ PD+YTYTSLI GE  + NVD A+ LF+EM   
Sbjct: 509 VMIYAYCKQGKIKEARKLRANMEANGMDPDSYTYTSLIHGECIADNVDEAMRLFSEMGLK 568

Query: 542 GLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
           GL++N VTYTV+ISGLSK G++DEAF LYDEM ++G   D+ +Y++LI S+H
Sbjct: 569 GLDQNSVTYTVMISGLSKAGKSDEAFGLYDEMKRKGYTIDNKVYTALIGSMH 620

BLAST of Cucsa.306820.1 vs. Swiss-Prot
Match: PP407_ARATH (Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana GN=EMB2745 PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.6e-71
Identity = 150/460 (32.61%), Postives = 259/460 (56.30%), Query Frame = 1

Query: 126 DMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSG-NMELCVEFLRQMV 185
           D++ + Y    + D +L +   A+  GF     S    L A  RS  N+       ++M+
Sbjct: 138 DLVVKSYSRLSLIDKALSIVHLAQAHGFMPGVLSYNAVLDATIRSKRNISFAENVFKEML 197

Query: 186 DSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVG 245
           +S +   V ++  ++ G C  G +  A  L D++  KG  P+V+TYNTL++GY +++ + 
Sbjct: 198 ESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKID 257

Query: 246 GVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSII 305
              ++L  M    ++ N+ +Y ++I    R  +++E   +  EM ++G   D   Y ++I
Sbjct: 258 DGFKLLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLI 317

Query: 306 NWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVD 365
              CK GN  +A V+  EM    L P+  TY +LI+  CKAG M  A   ++ M+ +G+ 
Sbjct: 318 KGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLC 377

Query: 366 VNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRL 425
            N   + TL+DG+ +KG ++EA R+   M   GF     T N + +G C + + E+A  +
Sbjct: 378 PNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAV 437

Query: 426 LLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCK 485
           L  M+E+G++P+VVS+S ++  +C+  +  EA R+ + M +KG  P  +TY++ I+ +C+
Sbjct: 438 LEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCE 497

Query: 486 KGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVT 545
           + + +EA  L  EM   GL PD +TYT+LI+     G++++AL+L NEM + G+  +VVT
Sbjct: 498 QRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVT 557

Query: 546 YTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 585
           Y+V+I+GL+K  R  EA +L  ++  E  VP D  Y +LI
Sbjct: 558 YSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLI 597


HSP 2 Score: 228.8 bits (582), Expect = 1.6e-58
Identity = 133/433 (30.72%), Postives = 225/433 (51.96%), Query Frame = 1

Query: 170 SGNMELCVEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVIT 229
           +GN+++ +    +M   G    V ++  ++DG CK  ++     L+  +  KG +P++I+
Sbjct: 218 AGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLIS 277

Query: 230 YNTLLNGYIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEML 289
           YN ++NG      +  V+ +L+ M +     +  TY  LI+ Y +     +A  +  EML
Sbjct: 278 YNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEML 337

Query: 290 KKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMK 349
           + G+ P V  YTS+I+  CK GNM RA    D+M  R L PN  TY  L++G  + G M 
Sbjct: 338 RHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMN 397

Query: 350 AAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIA 409
            A  ++ +M   G   + V +N L++G+C  G +++A+ +   M++KG   D  + + + 
Sbjct: 398 EAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVL 457

Query: 410 SGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKA 469
           SGFCRS   +EA R+   M E+G+ P+ +++S LI  +C+++   EA  L++ M + G  
Sbjct: 458 SGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLP 517

Query: 470 PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA--- 529
           P   TY A I  YC +G +E+A +L NEM E+G++PD  TY+ LI+G         A   
Sbjct: 518 PDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRL 577

Query: 530 -LELFNEMPQLGLNRNVVTYTVI--------------ISGLSKDGRADEAFKLYDEMNKE 585
            L+LF E     +  +V  +T+I              I G    G   EA ++++ M  +
Sbjct: 578 LLKLFYEE---SVPSDVTYHTLIENCSNIEFKSVVSLIKGFCMKGMMTEADQVFESMLGK 637


HSP 3 Score: 202.6 bits (514), Expect = 1.2e-50
Identity = 123/444 (27.70%), Postives = 216/444 (48.65%), Query Frame = 1

Query: 132 YMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIR 191
           Y   R  D   ++      KG E +  S    +  L R G M+     L +M   G  + 
Sbjct: 250 YCKLRKIDDGFKLLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLD 309

Query: 192 VCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILS 251
             ++  ++ G CK+G   +A  +  E++  G  PSVITY +L++   +  ++    E L 
Sbjct: 310 EVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLD 369

Query: 252 LMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFG 311
            M    +  N  TYT L++ +S+   + EA ++  EM   G  P V  Y ++IN +C  G
Sbjct: 370 QMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTG 429

Query: 312 NMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFN 371
            M+ A  + ++M E+ L P+  +Y  +++G C++ ++  A  +  +M  KG+  + + ++
Sbjct: 430 KMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYS 489

Query: 372 TLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEER 431
           +L+ G+C++    EA  L   M + G   D FT   + + +C     E+A +L   M E+
Sbjct: 490 SLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEK 549

Query: 432 GVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIER---------- 491
           GV P+VV++S+LI+   K+    EA+RL   +  +   PS VTY+  IE           
Sbjct: 550 GVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFKSVV 609

Query: 492 -----YCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQL 551
                +C KG M EA ++   M  +   PD   Y  +I G   +G++ +A  L+ EM + 
Sbjct: 610 SLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEMVKS 669

Query: 552 GLNRNVVTYTVIISGLSKDGRADE 561
           G   + VT   ++  L K+G+ +E
Sbjct: 670 GFLLHTVTVIALVKALHKEGKVNE 693


HSP 4 Score: 168.7 bits (426), Expect = 1.9e-40
Identity = 118/441 (26.76%), Postives = 214/441 (48.53%), Query Frame = 1

Query: 119 SIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVE 178
           S+ E   + L + Y     F  +L +     + G      +    + ++ ++GNM   +E
Sbjct: 307 SLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAME 366

Query: 179 FLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYI 238
           FL QM   G+     ++T +VDG  +KG +  A  ++ E+   GF PSV+TYN L+NG+ 
Sbjct: 367 FLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHC 426

Query: 239 EIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVY 298
               +     +L  M++  +  +V +Y+ ++  + RS  ++EA ++  EM++KGI+PD  
Sbjct: 427 VTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTI 486

Query: 299 IYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDM 358
            Y+S+I   C+    K A  L++EM    L P+ +TY ALIN  C  G+++ A  + N+M
Sbjct: 487 TYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEM 546

Query: 359 QSKGVDVNRVIFNTLMDGYCKKGMIDEALRL-------QNIMQQKGFEIDAFTCNII--- 418
             KGV  + V ++ L++G  K+    EA RL       +++     +      C+ I   
Sbjct: 547 VEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFK 606

Query: 419 -----ASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVM 478
                  GFC      EA ++  +M  +   P+  +++I+I  +C+  +  +A  L+K M
Sbjct: 607 SVVSLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEM 666

Query: 479 EKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNV 538
            K G     VT  A ++   K+GK+ E   +I  +     + +      L++     GN+
Sbjct: 667 VKSGFLLHTVTVIALVKALHKEGKVNELNSVIVHVLRSCELSEAEQAKVLVEINHREGNM 726

Query: 539 DRALELFNEMPQLGLNRNVVT 545
           D  L++  EM + G   N ++
Sbjct: 727 DVVLDVLAEMAKDGFLPNGIS 747

BLAST of Cucsa.306820.1 vs. Swiss-Prot
Match: PPR27_ARATH (Pentatricopeptide repeat-containing protein At1g09820 OS=Arabidopsis thaliana GN=At1g09820 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 7.4e-69
Identity = 169/552 (30.62%), Postives = 292/552 (52.90%), Query Frame = 1

Query: 38  QLVLS--DPNLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIV 97
           QL+ S  DP+L    CLR++ +L +N      L     L   L  A++++++++ L   V
Sbjct: 73  QLISSELDPDL----CLRYYSWLVKNSDISVSLELTFKLLHSLANAKRYSKIRSFLDGFV 132

Query: 98  NDGNLWSNVERIVSSIGGEFNEPSIVEKFC------DMLFRVYMDNRMFDSSLEVFDYAR 157
            +G+     +  V SI   F+  S+ +  C      DML   Y +N  F+   E F  + 
Sbjct: 133 RNGS-----DHQVHSI---FHAISMCDNVCVNSIIADMLVLAYANNSRFELGFEAFKRSG 192

Query: 158 KKGFEIDERSCFEFLLALKRSGNMELCVEFL-RQMVDSGIEIRVCSWTAVVDGLCKKGEV 217
             G+++   SC   ++AL +  N    VE++ ++M+   I+  V ++  V++ LCK G++
Sbjct: 193 YYGYKLSALSCKPLMIALLKE-NRSADVEYVYKEMIRRKIQPNVFTFNVVINALCKTGKM 252

Query: 218 VRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGV---NEILSLMEKNVVDYNVTTY 277
            +A+ +M+++   G  P+V++YNTL++GY ++   G +   + +L  M +N V  N+TT+
Sbjct: 253 NKARDVMEDMKVYGCSPNVVSYNTLIDGYCKLGGNGKMYKADAVLKEMVENDVSPNLTTF 312

Query: 278 TMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTE 337
            +LI+ + +   +  + K+F EML + ++P+V  Y S+IN  C  G +  A  + D+M  
Sbjct: 313 NILIDGFWKDDNLPGSMKVFKEMLDQDVKPNVISYNSLINGLCNGGKISEAISMRDKMVS 372

Query: 338 RRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDE 397
             + PN  TY ALING CK   +K A  M   ++ +G      ++N L+D YCK G ID+
Sbjct: 373 AGVQPNLITYNALINGFCKNDMLKEALDMFGSVKGQGAVPTTRMYNMLIDAYCKLGKIDD 432

Query: 398 ALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILID 457
              L+  M+++G   D  T N + +G CR+   E AK+L   +  +G+ P++V+F IL++
Sbjct: 433 GFALKEEMEREGIVPDVGTYNCLIAGLCRNGNIEAAKKLFDQLTSKGL-PDLVTFHILME 492

Query: 458 IYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQ-ERGLM 517
            YC++    +A  L K M K G  P  +TYN  ++ YCK+G ++ A  +  +M+ ER L 
Sbjct: 493 GYCRKGESRKAAMLLKEMSKMGLKPRHLTYNIVMKGYCKEGNLKAATNMRTQMEKERRLR 552

Query: 518 PDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKL 577
            +  +Y  L+ G    G ++ A  L NEM + GL  N +TY ++                
Sbjct: 553 MNVASYNVLLQGYSQKGKLEDANMLLNEMLEKGLVPNRITYEIV---------------- 594

BLAST of Cucsa.306820.1 vs. Swiss-Prot
Match: PP152_ARATH (Pentatricopeptide repeat-containing protein At2g15630, mitochondrial OS=Arabidopsis thaliana GN=At2g15630 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.8e-68
Identity = 146/462 (31.60%), Postives = 243/462 (52.60%), Query Frame = 1

Query: 126 DMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVD 185
           D+L R     RM D ++E F   ++KGF     +C   L  L R   +E    F   M  
Sbjct: 159 DLLVRCCCQLRMVDEAIECFYLMKEKGFYPKTETCNHILTLLSRLNRIENAWVFYADMYR 218

Query: 186 SGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGG 245
             I+  V ++  +++ LCK+G++ +AK  +  +   G KP+++TYNTL+ G+     + G
Sbjct: 219 MEIKSNVYTFNIMINVLCKEGKLKKAKGFLGIMEVFGIKPTIVTYNTLVQGFSLRGRIEG 278

Query: 246 VNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIIN 305
              I+S M+      ++ TY  ++ W     +   A ++  EM + G+ PD   Y  +I 
Sbjct: 279 ARLIISEMKSKGFQPDMQTYNPILSWMCNEGR---ASEVLREMKEIGLVPDSVSYNILIR 338

Query: 306 WNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDV 365
                G+++ AF   DEM ++ +VP  YTY  LI+G     +++AAE+++ +++ KG+ +
Sbjct: 339 GCSNNGDLEMAFAYRDEMVKQGMVPTFYTYNTLIHGLFMENKIEAAEILIREIREKGIVL 398

Query: 366 NRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLL 425
           + V +N L++GYC+ G   +A  L + M   G +   FT   +    CR N+  EA  L 
Sbjct: 399 DSVTYNILINGYCQHGDAKKAFALHDEMMTDGIQPTQFTYTSLIYVLCRKNKTREADELF 458

Query: 426 LTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKK 485
             +  +G+ P++V  + L+D +C   N   A  L K M+     P  VTYN  +   C +
Sbjct: 459 EKVVGKGMKPDLVMMNTLMDGHCAIGNMDRAFSLLKEMDMMSINPDDVTYNCLMRGLCGE 518

Query: 486 GKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTY 545
           GK EEA +L+ EM+ RG+ PD  +Y +LI G    G+   A  + +EM  LG N  ++TY
Sbjct: 519 GKFEEARELMGEMKRRGIKPDHISYNTLISGYSKKGDTKHAFMVRDEMLSLGFNPTLLTY 578

Query: 546 TVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 588
             ++ GLSK+   + A +L  EM  EGIVP+D  + S+I ++
Sbjct: 579 NALLKGLSKNQEGELAEELLREMKSEGIVPNDSSFCSVIEAM 617

BLAST of Cucsa.306820.1 vs. Swiss-Prot
Match: PPR26_ARATH (Putative pentatricopeptide repeat-containing protein At1g09680 OS=Arabidopsis thaliana GN=At1g09680 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-67
Identity = 150/539 (27.83%), Postives = 283/539 (52.50%), Query Frame = 1

Query: 23  KTTPSLLSNFDSRVMQLVLSDP-NLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRA 82
           K  PSL  +    V+ L+  +P +LP RS   FF F+   P  +  +  + +L   L   
Sbjct: 75  KVLPSLSVH---HVVDLINHNPLSLPQRSIFAFFKFISSQPGFRFTVETYFVLARFLAVH 134

Query: 83  RKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSIVEKFCDMLFRVYMDNRMFDSS 142
             F E +++++ +V+     S     +S +  E     +     D L   Y D      +
Sbjct: 135 EMFTEAQSLIELVVSRKGKNSASSVFISLV--EMRVTPMCGFLVDALMITYTDLGFIPDA 194

Query: 143 LEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIRVCSWTAVVDG 202
           ++ F  +RK  F++  R C   L  + +         F  +++D+G  + V  +  +++ 
Sbjct: 195 IQCFRLSRKHRFDVPIRGCGNLLDRMMKLNPTGTIWGFYMEILDAGFPLNVYVFNILMNK 254

Query: 203 LCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILSLMEKNVVDYN 262
            CK+G +  A+ + DE+  +  +P+V+++NTL+NGY ++ ++     +   MEK+    +
Sbjct: 255 FCKEGNISDAQKVFDEITKRSLQPTVVSFNTLINGYCKVGNLDEGFRLKHQMEKSRTRPD 314

Query: 263 VTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFD 322
           V TY+ LI    + +K++ A  LFDEM K+G+ P+  I+T++I+ + + G +      + 
Sbjct: 315 VFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFTTLIHGHSRNGEIDLMKESYQ 374

Query: 323 EMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKG 382
           +M  + L P+   Y  L+NG CK G++ AA  +V+ M  +G+  +++ + TL+DG+C+ G
Sbjct: 375 KMLSKGLQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRRGLRPDKITYTTLIDGFCRGG 434

Query: 383 MIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFS 442
            ++ AL ++  M Q G E+D    + +  G C+  R  +A+R L  M   G+ P+ V+++
Sbjct: 435 DVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDAERALREMLRAGIKPDDVTYT 494

Query: 443 ILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQER 502
           +++D +CK+ +     +L K M+  G  PSVVTYN  +   CK G+M+ A  L++ M   
Sbjct: 495 MMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVTYNVLLNGLCKLGQMKNADMLLDAMLNI 554

Query: 503 GLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGL---SKDGR 558
           G++PD  TY +L++G     N  +    + + P++G+  ++ +Y  I++ L   SKD R
Sbjct: 555 GVVPDDITYNTLLEGHHRHANSSKR---YIQKPEIGIVADLASYKSIVNELDRASKDHR 605


HSP 2 Score: 152.9 bits (385), Expect = 1.1e-35
Identity = 87/303 (28.71%), Postives = 152/303 (50.17%), Query Frame = 1

Query: 287 EMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAG 346
           E+L  G   +VY++  ++N  CK GN+  A  +FDE+T+R L P   ++  LING CK G
Sbjct: 230 EILDAGFPLNVYVFNILMNKFCKEGNISDAQKVFDEITKRSLQPTVVSFNTLINGYCKVG 289

Query: 347 EMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCN 406
            +     + + M+      +   ++ L++  CK+  +D A  L + M ++G   +     
Sbjct: 290 NLDEGFRLKHQMEKSRTRPDVFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFT 349

Query: 407 IIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKK 466
            +  G  R+   +  K     M  +G+ P++V ++ L++ +CK  +   AR +   M ++
Sbjct: 350 TLIHGHSRNGEIDLMKESYQKMLSKGLQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRR 409

Query: 467 GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 526
           G  P  +TY   I+ +C+ G +E A ++  EM + G+  D   +++L+ G    G V  A
Sbjct: 410 GLRPDKITYTTLIDGFCRGGDVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDA 469

Query: 527 LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIAS 586
                EM + G+  + VTYT+++    K G A   FKL  EM  +G VP    Y+ L+  
Sbjct: 470 ERALREMLRAGIKPDDVTYTMMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVTYNVLLNG 529

Query: 587 LHK 590
           L K
Sbjct: 530 LCK 532


HSP 3 Score: 121.3 bits (303), Expect = 3.5e-26
Identity = 65/241 (26.97%), Postives = 131/241 (54.36%), Query Frame = 1

Query: 344 KAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAF 403
           + G+  A+ + ++ ++ +   +   + + LM  Y   G I +A++   + ++  F++   
Sbjct: 147 RKGKNSASSVFISLVEMRVTPMCGFLVDALMITYTDLGFIPDAIQCFRLSRKHRFDVPIR 206

Query: 404 TCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVM 463
            C  +     + N         + + + G   NV  F+IL++ +CKE N ++A+++F  +
Sbjct: 207 GCGNLLDRMMKLNPTGTIWGFYMEILDAGFPLNVYVFNILMNKFCKEGNISDAQKVFDEI 266

Query: 464 EKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNV 523
            K+   P+VV++N  I  YCK G ++E ++L ++M++    PD +TY++LI+       +
Sbjct: 267 TKRSLQPTVVSFNTLINGYCKVGNLDEGFRLKHQMEKSRTRPDVFTYSALINALCKENKM 326

Query: 524 DRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSL 583
           D A  LF+EM + GL  N V +T +I G S++G  D   + Y +M  +G+ PD  +Y++L
Sbjct: 327 DGAHGLFDEMCKRGLIPNDVIFTTLIHGHSRNGEIDLMKESYQKMLSKGLQPDIVLYNTL 386

Query: 584 I 585
           +
Sbjct: 387 V 387

BLAST of Cucsa.306820.1 vs. TrEMBL
Match: A0A0A0LDI0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 3.1e-263
Identity = 469/528 (88.83%), Postives = 481/528 (91.10%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ
Sbjct: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60

Query: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120
           NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI
Sbjct: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120

Query: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180
           VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL
Sbjct: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180

Query: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240
           RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI
Sbjct: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240

Query: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300
           KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY
Sbjct: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300

Query: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360
           TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS
Sbjct: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360

Query: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420
           KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE
Sbjct: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420

Query: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480
           AKRLLLTMEERGVAPNVVSF    +      N   A  LF  M + G   +VVTY   I 
Sbjct: 421 AKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTVIIS 480

Query: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALE 529
              K G+ +EA+KL +EM + G++PD   Y+SLI      G +   LE
Sbjct: 481 GLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHKVGPLVSGLE 524

BLAST of Cucsa.306820.1 vs. TrEMBL
Match: A0A0A0LDI0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.7e-67
Identity = 142/342 (41.52%), Postives = 215/342 (62.87%), Query Frame = 1

Query: 248 EILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWN 307
           E L  M  + ++  V ++T +++   +  ++  A+ L DE++ KG +P V  Y +++N  
Sbjct: 178 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 237

Query: 308 CKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNR 367
            +  ++     +   M +  +  N  TY  LI    ++ +++ AE + ++M  KG++ + 
Sbjct: 238 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 297

Query: 368 VIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLT 427
            I+ ++++  CK G +  A  L + M ++    +A+T   + +G C++   + A+ ++  
Sbjct: 298 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 357

Query: 428 MEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGK 487
           M+ +GV  N V F+ L+D YCK+    EA RL  +M++KG      T N     +C+  +
Sbjct: 358 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 417

Query: 488 MEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTV 547
            EEA +L+  M+ERG+ P+  ++    +GERASGNVDRALELFNEMPQLGLNRNVVTYTV
Sbjct: 418 REEAKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTV 477

Query: 548 IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK
Sbjct: 478 IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 515


HSP 2 Score: 148.7 bits (374), Expect = 2.3e-32
Identity = 85/310 (27.42%), Postives = 160/310 (51.61%), Query Frame = 1

Query: 267 MLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTER 326
           ML   Y  +   + + ++FD   KKG E D       +    + GNM+       +M + 
Sbjct: 127 MLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDS 186

Query: 327 RLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEA 386
            +     ++ A+++G CK GE+  A+ +++++  KG   + + +NTL++GY +   +   
Sbjct: 187 GIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGV 246

Query: 387 LRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDI 446
             + ++M++   + +  T  ++   + RS++ EEA++L   M ++G+ P+V  ++ +I+ 
Sbjct: 247 NEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINW 306

Query: 447 YCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPD 506
            CK  N   A  LF  M ++   P+  TY A I   CK G+M+ A  ++N+MQ +G+  +
Sbjct: 307 NCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVN 366

Query: 507 TYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYD 566
              + +L+DG    G +D AL L N M Q G   +  T  +I SG  +  R +EA +L  
Sbjct: 367 RVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLL 426

Query: 567 EMNKEGIVPD 577
            M + G+ P+
Sbjct: 427 TMEERGVAPN 436


HSP 3 Score: 700.7 bits (1807), Expect = 1.6e-198
Identity = 347/589 (58.91%), Postives = 444/589 (75.38%), Query Frame = 1

Query: 4   QAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQNPS 63
           Q IA  I K+++KSGL+P +T  SLLSN DS    LVLS+PNLPTRSCL FF  L++N S
Sbjct: 24  QEIAEKITKILIKSGLKPLETKSSLLSNLDSYTTNLVLSNPNLPTRSCLNFFKCLQKNQS 83

Query: 64  ---RKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 123
               KPDL AH+IL SRL++ARKF  MKNVL     D NL  +V   VS I   F+EP  
Sbjct: 84  LICHKPDLRAHVILISRLFKARKFVVMKNVLTCYAMDKNLRCSVSDFVSLIDNRFHEPKF 143

Query: 124 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 183
           VEK CDMLFRVY+DN MF+   +VFDY    G +ID+RSC   LLALKRS  M + + F 
Sbjct: 144 VEKLCDMLFRVYVDNSMFEEGFKVFDYMVHNGLKIDDRSCIVCLLALKRSDQMVMSLGFF 203

Query: 184 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 243
           ++MV+  +++ V S T V+DGLCKKG V RAK LM E+  KG KP+V+TYNTL+N YI+I
Sbjct: 204 KKMVEFKVDVTVYSMTIVIDGLCKKGRVERAKDLMLEMTGKGIKPNVVTYNTLVNAYIKI 263

Query: 244 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 303
            D  GVNE+L LME + V YN  TYT+LIEWY  S KI EAEK+F++ML++G+E D++++
Sbjct: 264 MDFEGVNEMLRLMEMDKVVYNAATYTLLIEWYGSSGKIAEAEKVFEKMLERGVEADIHVF 323

Query: 304 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 363
           TSII+W CK GNMKRAF LFDE+ ER LV NA+TYGALI+G C +G++ AAEM+VN+MQS
Sbjct: 324 TSIISWQCKLGNMKRAFALFDELNERGLVANAHTYGALIHGTCNSGQLDAAEMLVNEMQS 383

Query: 364 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 423
           +G+D+N VIFNTL++GYCKKGMIDEALR+Q++M++KGFE D FT N IA G C+ NR EE
Sbjct: 384 QGLDMNLVIFNTLINGYCKKGMIDEALRMQDVMEKKGFENDIFTYNTIAGGLCKLNRHEE 443

Query: 424 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 483
           AKR L TM E+GV PN VSF+I+IDI+CKE N  EA R+F+ M+KKG+ P+VVTYN  I+
Sbjct: 444 AKRWLFTMVEKGVDPNAVSFTIMIDIHCKEGNLVEAERVFQDMKKKGEKPNVVTYNTLID 503

Query: 484 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR 543
            Y KKGK++EAY+L +EM+  G+  D YTYT+L+ GE   G V+ AL L NE+ + GL  
Sbjct: 504 GYSKKGKLKEAYRLKDEMESIGMTSDIYTYTTLVHGECVFGKVEEALTLLNEVCRKGLAI 563

Query: 544 NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           ++VTYT IISGLSK+GR++EAF+LYDEM   G+ PDD +Y+SL+A+LHK
Sbjct: 564 SIVTYTAIISGLSKEGRSEEAFRLYDEMMAAGLTPDDRVYTSLVANLHK 612

BLAST of Cucsa.306820.1 vs. TrEMBL
Match: V4S3K8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011284mg PE=4 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.7e-195
Identity = 333/588 (56.63%), Postives = 440/588 (74.83%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           +S + I+  IAK +  SG++PFKTTPSLLSN +S +  LVLSD NLP  +CL FF+FLRQ
Sbjct: 21  ISYKDISTQIAKTLFNSGIEPFKTTPSLLSNLNSHITHLVLSDTNLPPHACLHFFNFLRQ 80

Query: 61  NPS---RKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNE 120
           NPS    +PDL AH+ L  RL+ A+KFAEMK V  +IV D NL  ++  ++S I     +
Sbjct: 81  NPSLYSHRPDLQAHVTLICRLHEAQKFAEMKKVFNYIVTDANLRCSIVNLISLIESGNQQ 140

Query: 121 PSIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCV 180
           P  VEKFCD+LFRVY DNR+F+ +L VFDY  + G +IDERSC  FLLALKR  ++E+C 
Sbjct: 141 PKFVEKFCDLLFRVYADNRLFEDALGVFDYMERSGLKIDERSCIAFLLALKRCDHIEMCF 200

Query: 181 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 240
           +F R+MVD+ +EI V S + ++DGLCK+G+V  A+ LMDE+V KG KP++ TYNTLLNG+
Sbjct: 201 KFFRKMVDANVEITVYSISIMIDGLCKRGDVEMARDLMDEMVVKGIKPNLYTYNTLLNGF 260

Query: 241 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 300
           ++ KD  GVNE+LSLM+++ V YNV TYT+L +WY  S K+ E EK+FDEM ++G+E DV
Sbjct: 261 VKRKDAVGVNEMLSLMQRDQVAYNVVTYTLLTDWYGNSGKMVEVEKMFDEMRERGVEADV 320

Query: 301 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 360
           ++YT++I+WNCK GNM+RAF LFD++TER +VPN++ YGA+I+G CK G+++AAEM++ D
Sbjct: 321 HVYTTVISWNCKSGNMRRAFTLFDDLTERGIVPNSHIYGAMIDGVCKVGQVEAAEMLLKD 380

Query: 361 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 420
           MQ +G+DVN VIFNTLM+GYCKKGMIDEAL++  IM++KGFE   F  N IASG C+  R
Sbjct: 381 MQRQGLDVNEVIFNTLMNGYCKKGMIDEALKVMAIMEKKGFEAGPFAYNTIASGLCKVKR 440

Query: 421 REEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNA 480
             EAK+ L TM ERG+A N V+FS LIDI CKE N  EA+ LF+ M+ KG  P+  TYN 
Sbjct: 441 YNEAKKWLFTMMERGLALNTVNFSTLIDICCKEGNLEEAKWLFEDMKMKGVKPNSFTYNT 500

Query: 481 FIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLG 540
            I  Y KKG +++AY+L  +M+E G+ PD YTYTSLI GER  G +D A+ LFNEM + G
Sbjct: 501 LIHGYSKKGNIKQAYRLKCKMEEMGMTPDVYTYTSLIHGERVFGKLDEAMILFNEMIEKG 560

Query: 541 LNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 586
              N VTYT +I+GLSK+GR+DEAF+LYDEM   G+ PDD + SSL+A
Sbjct: 561 FIPNAVTYTTLITGLSKEGRSDEAFRLYDEMIASGLTPDDKLCSSLVA 608

BLAST of Cucsa.306820.1 vs. TrEMBL
Match: A0A067DGQ8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g047794mg PE=4 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 6.1e-195
Identity = 333/588 (56.63%), Postives = 439/588 (74.66%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           +S + I+  IAK +  SG++PFKTTPSLLSN +S +  LVLSD NLP  +CL FF+FLRQ
Sbjct: 21  ISYKDISTQIAKTLFNSGIEPFKTTPSLLSNLNSHITHLVLSDTNLPPHACLHFFNFLRQ 80

Query: 61  NPS---RKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNE 120
           NPS    +PDL AH+ L  RLY A+KFAEMK V  +IV D NL  ++  ++S I     +
Sbjct: 81  NPSLYSHRPDLQAHVTLICRLYEAQKFAEMKKVFNYIVTDANLRCSIVNLISLIESGNQQ 140

Query: 121 PSIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCV 180
           P  VEKFCD+LFRVY DNR+F+ +L VFDY  + G +IDERSC  FLLALKR  ++E+C 
Sbjct: 141 PKFVEKFCDLLFRVYADNRLFEDALGVFDYMERSGLKIDERSCIAFLLALKRCDHIEMCF 200

Query: 181 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 240
           +F R+MVD+ +EI V S + ++DGLCK+G+V  A+ LMDE+V KG KP++ TYNTLLNG+
Sbjct: 201 KFFRKMVDANVEITVYSISIMIDGLCKRGDVEMARDLMDEMVVKGIKPNLYTYNTLLNGF 260

Query: 241 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 300
           ++ KD  GVNE+LSLM+++ V YNV TYT+L +WY  S K+ E EK+FDEM ++G+E DV
Sbjct: 261 VKRKDAVGVNEMLSLMQRDQVAYNVVTYTLLTDWYGNSGKMVEVEKMFDEMHERGVEADV 320

Query: 301 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 360
           ++YT++I+WNCK GNM+RAF LFD++TER +VPN++ YGA+I+G CK G+++AAEM++ D
Sbjct: 321 HVYTTVISWNCKSGNMRRAFTLFDDLTERGIVPNSHIYGAMIDGVCKVGQVEAAEMLLKD 380

Query: 361 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 420
           MQ +G+DVN VIFNTLM+GYCKKGMIDEAL++  IM++KGFE   F  N IASG C+  R
Sbjct: 381 MQRQGLDVNEVIFNTLMNGYCKKGMIDEALKVMAIMEKKGFEAGPFAYNTIASGLCKVKR 440

Query: 421 REEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNA 480
             EAK+ L TM ERG+A N V+FS LI I CKE N  EA+ LF+ M+ KG  P+  TYN 
Sbjct: 441 YNEAKKWLFTMMERGLALNTVNFSTLIGICCKEGNLEEAKWLFEDMKMKGVKPNSFTYNT 500

Query: 481 FIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLG 540
            I  Y KKG +++AY+L  +M+E G+ PD YTYTSLI GER  G +D A+ LFNEM + G
Sbjct: 501 LIHGYSKKGNIKQAYRLKCKMEEMGMTPDVYTYTSLIHGERVFGKLDEAMILFNEMIEKG 560

Query: 541 LNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIA 586
              N VTYT +I+GLSK+GR+DEAF+LYDEM   G+ PDD + SSL+A
Sbjct: 561 FIPNAVTYTTLITGLSKEGRSDEAFRLYDEMIASGLTPDDKLCSSLVA 608

BLAST of Cucsa.306820.1 vs. TrEMBL
Match: A0A165WT71_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_014992 PE=4 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 2.0e-190
Identity = 327/590 (55.42%), Postives = 441/590 (74.75%), Query Frame = 1

Query: 2   SKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQN 61
           S + +A  IAK ++ S  +P +  PSL SN +  ++ LVLS+PN+   SCL FF FL+ N
Sbjct: 22  SDERLAKLIAKTLINSTSKPLQIDPSLTSNLNPNIIHLVLSNPNVKIPSCLHFFRFLKSN 81

Query: 62  P---SRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEP 121
           P   S+KP+L AH+ L  RL++ARKFAE K +L  +V++ NL      IVS +   + EP
Sbjct: 82  PYPFSQKPNLQAHITLVCRLFQARKFAEAKIILNQVVSNENLVCPTSDIVSMMIESYEEP 141

Query: 122 SIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVE 181
            IV K  DMLFRVY DN+ F+  LE+F+Y +  GFEID+RSC  +LLA+KR   ++L  +
Sbjct: 142 KIVHKLFDMLFRVYADNKRFEECLEIFEYMKSNGFEIDDRSCMVYLLAVKRCDQLDLLYD 201

Query: 182 FLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYI 241
           F R+MVDSG++I V S T V+DGLCK GEV +A+ L+DE+V +G +P+  TYNTL++ Y+
Sbjct: 202 FFRRMVDSGVKITVYSMTLVIDGLCKIGEVGKARNLLDEMVDRGIEPNDYTYNTLIDAYM 261

Query: 242 EIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVY 301
           +  D GGV E+L+ MEKN + YNV TYT+LI W SRS ++EEAE+LF+EM ++GI  DV+
Sbjct: 262 KKLDSGGVKEMLNAMEKNGIPYNVATYTLLINWNSRSEEMEEAERLFEEMNQRGILGDVH 321

Query: 302 IYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDM 361
           +YT++I+WNCK GN K+AF LFDEM ER LVPN +TY AL+NG CKAG M+  E+++++M
Sbjct: 322 VYTTMISWNCKLGNTKKAFRLFDEMAERGLVPNCHTYAALVNGLCKAGHMEGVEVLLHEM 381

Query: 362 QSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRR 421
           Q+KG ++NRVIFNTL+DGYCK+GMIDEA R Q+IM+QKGFE D F  + IA G C+ NR 
Sbjct: 382 QTKGYNLNRVIFNTLIDGYCKRGMIDEAWRCQSIMEQKGFEADVFAYSTIAHGLCKLNRH 441

Query: 422 EEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAF 481
           EEAKRLLL+M +RGVAPN+V F+ LIDIYCK+ +F EA+R  + ME KG+ P+VVTYNA 
Sbjct: 442 EEAKRLLLSMVDRGVAPNIVVFTTLIDIYCKQGDFVEAKRTIREMESKGEKPNVVTYNAL 501

Query: 482 IERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGL 541
           I+ YCKK  M+EA+K + EM+ +GL+ D YTYTSL+ GE  SGN+D A  LF+EMP   L
Sbjct: 502 IDGYCKKSMMKEAHKTMREMEVKGLVADVYTYTSLVHGECISGNIDEAARLFSEMPDKRL 561

Query: 542 NRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
             N++TY+V+ISGLSK GR+DEAFKLYD+M + G+ PDD +Y++L+ SLH
Sbjct: 562 VPNIITYSVMISGLSKVGRSDEAFKLYDQMKEAGLTPDDKVYAALVGSLH 611

BLAST of Cucsa.306820.1 vs. TAIR10
Match: AT2G32630.1 (AT2G32630.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 612.5 bits (1578), Expect = 2.8e-175
Identity = 312/592 (52.70%), Postives = 425/592 (71.79%), Query Frame = 1

Query: 2   SKQAIAANI-AKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 61
           S Q  A  I A L+ KS +   ++ PSLL N +S V +LVLS+P LPT+SC+ FF  LR+
Sbjct: 29  SDQEAARRITACLVEKSTIGKLQSNPSLLFNLNSNVTRLVLSEPTLPTQSCIDFFKLLRE 88

Query: 62  NPSR-KPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSS-IGGEFNEP 121
             S  KPDL A + L  RLY  R+F EM+++L  +VNDG     VE + S+ +  + +E 
Sbjct: 89  FESNLKPDLTAVVTLSHRLYSNRRFNEMRSLLNSVVNDGFYKRPVEELGSAMVDCDISEE 148

Query: 122 SI--VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELC 181
                EKF D++FRVY+DN MF+  L VFDY  KKG  IDERSC  FL+A K+   ++LC
Sbjct: 149 KFEFFEKFFDLVFRVYVDNGMFEEGLRVFDYMVKKGLSIDERSCIVFLVAAKKRRRIDLC 208

Query: 182 VEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNG 241
           +E  R+MVDSG++I V S T VV+GLC++GEV ++K L+ E   KG KP   TYNT++N 
Sbjct: 209 LEIFRRMVDSGVKITVYSLTIVVEGLCRRGEVEKSKKLIKEFSVKGIKPEAYTYNTIINA 268

Query: 242 YIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPD 301
           Y++ +D  GV  +L +M+K+ V YN  TYT+L+E   ++ K+ +AEKLFDEM ++GIE D
Sbjct: 269 YVKQRDFSGVEGVLKVMKKDGVVYNKVTYTLLMELSVKNGKMSDAEKLFDEMRERGIESD 328

Query: 302 VYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVN 361
           V++YTS+I+WNC+ GNMKRAF+LFDE+TE+ L P++YTYGALI+G CK GEM AAE+++N
Sbjct: 329 VHVYTSLISWNCRKGNMKRAFLLFDELTEKGLSPSSYTYGALIDGVCKVGEMGAAEILMN 388

Query: 362 DMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSN 421
           +MQSKGV++ +V+FNTL+DGYC+KGM+DEA  + ++M+QKGF+ D FTCN IAS F R  
Sbjct: 389 EMQSKGVNITQVVFNTLIDGYCRKGMVDEASMIYDVMEQKGFQADVFTCNTIASCFNRLK 448

Query: 422 RREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYN 481
           R +EAK+ L  M E GV  + VS++ LID+YCKE N  EA+RLF  M  KG  P+ +TYN
Sbjct: 449 RYDEAKQWLFRMMEGGVKLSTVSYTNLIDVYCKEGNVEEAKRLFVEMSSKGVQPNAITYN 508

Query: 482 AFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQL 541
             I  YCK+GK++EA KL   M+  G+ PD+YTYTSLI GE  + NVD A+ LF+EM   
Sbjct: 509 VMIYAYCKQGKIKEARKLRANMEANGMDPDSYTYTSLIHGECIADNVDEAMRLFSEMGLK 568

Query: 542 GLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
           GL++N VTYTV+ISGLSK G++DEAF LYDEM ++G   D+ +Y++LI S+H
Sbjct: 569 GLDQNSVTYTVMISGLSKAGKSDEAFGLYDEMKRKGYTIDNKVYTALIGSMH 620

BLAST of Cucsa.306820.1 vs. TAIR10
Match: AT5G39710.1 (AT5G39710.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 270.8 bits (691), Expect = 2.0e-72
Identity = 150/460 (32.61%), Postives = 259/460 (56.30%), Query Frame = 1

Query: 126 DMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSG-NMELCVEFLRQMV 185
           D++ + Y    + D +L +   A+  GF     S    L A  RS  N+       ++M+
Sbjct: 138 DLVVKSYSRLSLIDKALSIVHLAQAHGFMPGVLSYNAVLDATIRSKRNISFAENVFKEML 197

Query: 186 DSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVG 245
           +S +   V ++  ++ G C  G +  A  L D++  KG  P+V+TYNTL++GY +++ + 
Sbjct: 198 ESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKID 257

Query: 246 GVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSII 305
              ++L  M    ++ N+ +Y ++I    R  +++E   +  EM ++G   D   Y ++I
Sbjct: 258 DGFKLLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLI 317

Query: 306 NWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVD 365
              CK GN  +A V+  EM    L P+  TY +LI+  CKAG M  A   ++ M+ +G+ 
Sbjct: 318 KGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLC 377

Query: 366 VNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRL 425
            N   + TL+DG+ +KG ++EA R+   M   GF     T N + +G C + + E+A  +
Sbjct: 378 PNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAV 437

Query: 426 LLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCK 485
           L  M+E+G++P+VVS+S ++  +C+  +  EA R+ + M +KG  P  +TY++ I+ +C+
Sbjct: 438 LEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCE 497

Query: 486 KGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVT 545
           + + +EA  L  EM   GL PD +TYT+LI+     G++++AL+L NEM + G+  +VVT
Sbjct: 498 QRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVT 557

Query: 546 YTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLI 585
           Y+V+I+GL+K  R  EA +L  ++  E  VP D  Y +LI
Sbjct: 558 YSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLI 597


HSP 2 Score: 228.8 bits (582), Expect = 8.7e-60
Identity = 133/433 (30.72%), Postives = 225/433 (51.96%), Query Frame = 1

Query: 170 SGNMELCVEFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVIT 229
           +GN+++ +    +M   G    V ++  ++DG CK  ++     L+  +  KG +P++I+
Sbjct: 218 AGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLIS 277

Query: 230 YNTLLNGYIEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEML 289
           YN ++NG      +  V+ +L+ M +     +  TY  LI+ Y +     +A  +  EML
Sbjct: 278 YNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEML 337

Query: 290 KKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMK 349
           + G+ P V  YTS+I+  CK GNM RA    D+M  R L PN  TY  L++G  + G M 
Sbjct: 338 RHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMN 397

Query: 350 AAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIA 409
            A  ++ +M   G   + V +N L++G+C  G +++A+ +   M++KG   D  + + + 
Sbjct: 398 EAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVL 457

Query: 410 SGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKA 469
           SGFCRS   +EA R+   M E+G+ P+ +++S LI  +C+++   EA  L++ M + G  
Sbjct: 458 SGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLP 517

Query: 470 PSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA--- 529
           P   TY A I  YC +G +E+A +L NEM E+G++PD  TY+ LI+G         A   
Sbjct: 518 PDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRL 577

Query: 530 -LELFNEMPQLGLNRNVVTYTVI--------------ISGLSKDGRADEAFKLYDEMNKE 585
            L+LF E     +  +V  +T+I              I G    G   EA ++++ M  +
Sbjct: 578 LLKLFYEE---SVPSDVTYHTLIENCSNIEFKSVVSLIKGFCMKGMMTEADQVFESMLGK 637


HSP 3 Score: 202.6 bits (514), Expect = 6.7e-52
Identity = 123/444 (27.70%), Postives = 216/444 (48.65%), Query Frame = 1

Query: 132 YMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIR 191
           Y   R  D   ++      KG E +  S    +  L R G M+     L +M   G  + 
Sbjct: 250 YCKLRKIDDGFKLLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLD 309

Query: 192 VCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILS 251
             ++  ++ G CK+G   +A  +  E++  G  PSVITY +L++   +  ++    E L 
Sbjct: 310 EVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLD 369

Query: 252 LMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFG 311
            M    +  N  TYT L++ +S+   + EA ++  EM   G  P V  Y ++IN +C  G
Sbjct: 370 QMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTG 429

Query: 312 NMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFN 371
            M+ A  + ++M E+ L P+  +Y  +++G C++ ++  A  +  +M  KG+  + + ++
Sbjct: 430 KMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYS 489

Query: 372 TLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEER 431
           +L+ G+C++    EA  L   M + G   D FT   + + +C     E+A +L   M E+
Sbjct: 490 SLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEK 549

Query: 432 GVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIER---------- 491
           GV P+VV++S+LI+   K+    EA+RL   +  +   PS VTY+  IE           
Sbjct: 550 GVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFKSVV 609

Query: 492 -----YCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQL 551
                +C KG M EA ++   M  +   PD   Y  +I G   +G++ +A  L+ EM + 
Sbjct: 610 SLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEMVKS 669

Query: 552 GLNRNVVTYTVIISGLSKDGRADE 561
           G   + VT   ++  L K+G+ +E
Sbjct: 670 GFLLHTVTVIALVKALHKEGKVNE 693


HSP 4 Score: 168.7 bits (426), Expect = 1.1e-41
Identity = 118/441 (26.76%), Postives = 214/441 (48.53%), Query Frame = 1

Query: 119 SIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVE 178
           S+ E   + L + Y     F  +L +     + G      +    + ++ ++GNM   +E
Sbjct: 307 SLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAME 366

Query: 179 FLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYI 238
           FL QM   G+     ++T +VDG  +KG +  A  ++ E+   GF PSV+TYN L+NG+ 
Sbjct: 367 FLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHC 426

Query: 239 EIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVY 298
               +     +L  M++  +  +V +Y+ ++  + RS  ++EA ++  EM++KGI+PD  
Sbjct: 427 VTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTI 486

Query: 299 IYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDM 358
            Y+S+I   C+    K A  L++EM    L P+ +TY ALIN  C  G+++ A  + N+M
Sbjct: 487 TYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEM 546

Query: 359 QSKGVDVNRVIFNTLMDGYCKKGMIDEALRL-------QNIMQQKGFEIDAFTCNII--- 418
             KGV  + V ++ L++G  K+    EA RL       +++     +      C+ I   
Sbjct: 547 VEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFK 606

Query: 419 -----ASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVM 478
                  GFC      EA ++  +M  +   P+  +++I+I  +C+  +  +A  L+K M
Sbjct: 607 SVVSLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEM 666

Query: 479 EKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNV 538
            K G     VT  A ++   K+GK+ E   +I  +     + +      L++     GN+
Sbjct: 667 VKSGFLLHTVTVIALVKALHKEGKVNELNSVIVHVLRSCELSEAEQAKVLVEINHREGNM 726

Query: 539 DRALELFNEMPQLGLNRNVVT 545
           D  L++  EM + G   N ++
Sbjct: 727 DVVLDVLAEMAKDGFLPNGIS 747

BLAST of Cucsa.306820.1 vs. TAIR10
Match: AT1G09820.1 (AT1G09820.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 263.1 bits (671), Expect = 4.2e-70
Identity = 169/552 (30.62%), Postives = 292/552 (52.90%), Query Frame = 1

Query: 38  QLVLS--DPNLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIV 97
           QL+ S  DP+L    CLR++ +L +N      L     L   L  A++++++++ L   V
Sbjct: 73  QLISSELDPDL----CLRYYSWLVKNSDISVSLELTFKLLHSLANAKRYSKIRSFLDGFV 132

Query: 98  NDGNLWSNVERIVSSIGGEFNEPSIVEKFC------DMLFRVYMDNRMFDSSLEVFDYAR 157
            +G+     +  V SI   F+  S+ +  C      DML   Y +N  F+   E F  + 
Sbjct: 133 RNGS-----DHQVHSI---FHAISMCDNVCVNSIIADMLVLAYANNSRFELGFEAFKRSG 192

Query: 158 KKGFEIDERSCFEFLLALKRSGNMELCVEFL-RQMVDSGIEIRVCSWTAVVDGLCKKGEV 217
             G+++   SC   ++AL +  N    VE++ ++M+   I+  V ++  V++ LCK G++
Sbjct: 193 YYGYKLSALSCKPLMIALLKE-NRSADVEYVYKEMIRRKIQPNVFTFNVVINALCKTGKM 252

Query: 218 VRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGV---NEILSLMEKNVVDYNVTTY 277
            +A+ +M+++   G  P+V++YNTL++GY ++   G +   + +L  M +N V  N+TT+
Sbjct: 253 NKARDVMEDMKVYGCSPNVVSYNTLIDGYCKLGGNGKMYKADAVLKEMVENDVSPNLTTF 312

Query: 278 TMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTE 337
            +LI+ + +   +  + K+F EML + ++P+V  Y S+IN  C  G +  A  + D+M  
Sbjct: 313 NILIDGFWKDDNLPGSMKVFKEMLDQDVKPNVISYNSLINGLCNGGKISEAISMRDKMVS 372

Query: 338 RRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDE 397
             + PN  TY ALING CK   +K A  M   ++ +G      ++N L+D YCK G ID+
Sbjct: 373 AGVQPNLITYNALINGFCKNDMLKEALDMFGSVKGQGAVPTTRMYNMLIDAYCKLGKIDD 432

Query: 398 ALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILID 457
              L+  M+++G   D  T N + +G CR+   E AK+L   +  +G+ P++V+F IL++
Sbjct: 433 GFALKEEMEREGIVPDVGTYNCLIAGLCRNGNIEAAKKLFDQLTSKGL-PDLVTFHILME 492

Query: 458 IYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQ-ERGLM 517
            YC++    +A  L K M K G  P  +TYN  ++ YCK+G ++ A  +  +M+ ER L 
Sbjct: 493 GYCRKGESRKAAMLLKEMSKMGLKPRHLTYNIVMKGYCKEGNLKAATNMRTQMEKERRLR 552

Query: 518 PDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKL 577
            +  +Y  L+ G    G ++ A  L NEM + GL  N +TY ++                
Sbjct: 553 MNVASYNVLLQGYSQKGKLEDANMLLNEMLEKGLVPNRITYEIV---------------- 594

BLAST of Cucsa.306820.1 vs. TAIR10
Match: AT2G15630.1 (AT2G15630.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 260.4 bits (664), Expect = 2.7e-69
Identity = 146/462 (31.60%), Postives = 243/462 (52.60%), Query Frame = 1

Query: 126 DMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVD 185
           D+L R     RM D ++E F   ++KGF     +C   L  L R   +E    F   M  
Sbjct: 159 DLLVRCCCQLRMVDEAIECFYLMKEKGFYPKTETCNHILTLLSRLNRIENAWVFYADMYR 218

Query: 186 SGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGG 245
             I+  V ++  +++ LCK+G++ +AK  +  +   G KP+++TYNTL+ G+     + G
Sbjct: 219 MEIKSNVYTFNIMINVLCKEGKLKKAKGFLGIMEVFGIKPTIVTYNTLVQGFSLRGRIEG 278

Query: 246 VNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIIN 305
              I+S M+      ++ TY  ++ W     +   A ++  EM + G+ PD   Y  +I 
Sbjct: 279 ARLIISEMKSKGFQPDMQTYNPILSWMCNEGR---ASEVLREMKEIGLVPDSVSYNILIR 338

Query: 306 WNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDV 365
                G+++ AF   DEM ++ +VP  YTY  LI+G     +++AAE+++ +++ KG+ +
Sbjct: 339 GCSNNGDLEMAFAYRDEMVKQGMVPTFYTYNTLIHGLFMENKIEAAEILIREIREKGIVL 398

Query: 366 NRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLL 425
           + V +N L++GYC+ G   +A  L + M   G +   FT   +    CR N+  EA  L 
Sbjct: 399 DSVTYNILINGYCQHGDAKKAFALHDEMMTDGIQPTQFTYTSLIYVLCRKNKTREADELF 458

Query: 426 LTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKK 485
             +  +G+ P++V  + L+D +C   N   A  L K M+     P  VTYN  +   C +
Sbjct: 459 EKVVGKGMKPDLVMMNTLMDGHCAIGNMDRAFSLLKEMDMMSINPDDVTYNCLMRGLCGE 518

Query: 486 GKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTY 545
           GK EEA +L+ EM+ RG+ PD  +Y +LI G    G+   A  + +EM  LG N  ++TY
Sbjct: 519 GKFEEARELMGEMKRRGIKPDHISYNTLISGYSKKGDTKHAFMVRDEMLSLGFNPTLLTY 578

Query: 546 TVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASL 588
             ++ GLSK+   + A +L  EM  EGIVP+D  + S+I ++
Sbjct: 579 NALLKGLSKNQEGELAEELLREMKSEGIVPNDSSFCSVIEAM 617

BLAST of Cucsa.306820.1 vs. TAIR10
Match: AT1G09680.1 (AT1G09680.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 258.8 bits (660), Expect = 7.9e-69
Identity = 150/539 (27.83%), Postives = 283/539 (52.50%), Query Frame = 1

Query: 23  KTTPSLLSNFDSRVMQLVLSDP-NLPTRSCLRFFDFLRQNPSRKPDLPAHLILFSRLYRA 82
           K  PSL  +    V+ L+  +P +LP RS   FF F+   P  +  +  + +L   L   
Sbjct: 75  KVLPSLSVH---HVVDLINHNPLSLPQRSIFAFFKFISSQPGFRFTVETYFVLARFLAVH 134

Query: 83  RKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSIVEKFCDMLFRVYMDNRMFDSS 142
             F E +++++ +V+     S     +S +  E     +     D L   Y D      +
Sbjct: 135 EMFTEAQSLIELVVSRKGKNSASSVFISLV--EMRVTPMCGFLVDALMITYTDLGFIPDA 194

Query: 143 LEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDSGIEIRVCSWTAVVDG 202
           ++ F  +RK  F++  R C   L  + +         F  +++D+G  + V  +  +++ 
Sbjct: 195 IQCFRLSRKHRFDVPIRGCGNLLDRMMKLNPTGTIWGFYMEILDAGFPLNVYVFNILMNK 254

Query: 203 LCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGVNEILSLMEKNVVDYN 262
            CK+G +  A+ + DE+  +  +P+V+++NTL+NGY ++ ++     +   MEK+    +
Sbjct: 255 FCKEGNISDAQKVFDEITKRSLQPTVVSFNTLINGYCKVGNLDEGFRLKHQMEKSRTRPD 314

Query: 263 VTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFD 322
           V TY+ LI    + +K++ A  LFDEM K+G+ P+  I+T++I+ + + G +      + 
Sbjct: 315 VFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFTTLIHGHSRNGEIDLMKESYQ 374

Query: 323 EMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKG 382
           +M  + L P+   Y  L+NG CK G++ AA  +V+ M  +G+  +++ + TL+DG+C+ G
Sbjct: 375 KMLSKGLQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRRGLRPDKITYTTLIDGFCRGG 434

Query: 383 MIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFS 442
            ++ AL ++  M Q G E+D    + +  G C+  R  +A+R L  M   G+ P+ V+++
Sbjct: 435 DVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDAERALREMLRAGIKPDDVTYT 494

Query: 443 ILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQER 502
           +++D +CK+ +     +L K M+  G  PSVVTYN  +   CK G+M+ A  L++ M   
Sbjct: 495 MMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVTYNVLLNGLCKLGQMKNADMLLDAMLNI 554

Query: 503 GLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGL---SKDGR 558
           G++PD  TY +L++G     N  +    + + P++G+  ++ +Y  I++ L   SKD R
Sbjct: 555 GVVPDDITYNTLLEGHHRHANSSKR---YIQKPEIGIVADLASYKSIVNELDRASKDHR 605


HSP 2 Score: 152.9 bits (385), Expect = 6.1e-37
Identity = 87/303 (28.71%), Postives = 152/303 (50.17%), Query Frame = 1

Query: 287 EMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAG 346
           E+L  G   +VY++  ++N  CK GN+  A  +FDE+T+R L P   ++  LING CK G
Sbjct: 230 EILDAGFPLNVYVFNILMNKFCKEGNISDAQKVFDEITKRSLQPTVVSFNTLINGYCKVG 289

Query: 347 EMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCN 406
            +     + + M+      +   ++ L++  CK+  +D A  L + M ++G   +     
Sbjct: 290 NLDEGFRLKHQMEKSRTRPDVFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFT 349

Query: 407 IIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKK 466
            +  G  R+   +  K     M  +G+ P++V ++ L++ +CK  +   AR +   M ++
Sbjct: 350 TLIHGHSRNGEIDLMKESYQKMLSKGLQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRR 409

Query: 467 GKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRA 526
           G  P  +TY   I+ +C+ G +E A ++  EM + G+  D   +++L+ G    G V  A
Sbjct: 410 GLRPDKITYTTLIDGFCRGGDVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDA 469

Query: 527 LELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIAS 586
                EM + G+  + VTYT+++    K G A   FKL  EM  +G VP    Y+ L+  
Sbjct: 470 ERALREMLRAGIKPDDVTYTMMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVTYNVLLNG 529

Query: 587 LHK 590
           L K
Sbjct: 530 LCK 532


HSP 3 Score: 121.3 bits (303), Expect = 2.0e-27
Identity = 65/241 (26.97%), Postives = 131/241 (54.36%), Query Frame = 1

Query: 344 KAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAF 403
           + G+  A+ + ++ ++ +   +   + + LM  Y   G I +A++   + ++  F++   
Sbjct: 147 RKGKNSASSVFISLVEMRVTPMCGFLVDALMITYTDLGFIPDAIQCFRLSRKHRFDVPIR 206

Query: 404 TCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVM 463
            C  +     + N         + + + G   NV  F+IL++ +CKE N ++A+++F  +
Sbjct: 207 GCGNLLDRMMKLNPTGTIWGFYMEILDAGFPLNVYVFNILMNKFCKEGNISDAQKVFDEI 266

Query: 464 EKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNV 523
            K+   P+VV++N  I  YCK G ++E ++L ++M++    PD +TY++LI+       +
Sbjct: 267 TKRSLQPTVVSFNTLINGYCKVGNLDEGFRLKHQMEKSRTRPDVFTYSALINALCKENKM 326

Query: 524 DRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSL 583
           D A  LF+EM + GL  N V +T +I G S++G  D   + Y +M  +G+ PD  +Y++L
Sbjct: 327 DGAHGLFDEMCKRGLIPNDVIFTTLIHGHSRNGEIDLMKESYQKMLSKGLQPDIVLYNTL 386

Query: 584 I 585
           +
Sbjct: 387 V 387

BLAST of Cucsa.306820.1 vs. NCBI nr
Match: gi|449440401|ref|XP_004137973.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Cucumis sativus])

HSP 1 Score: 1172.9 bits (3033), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ
Sbjct: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60

Query: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120
           NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI
Sbjct: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120

Query: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180
           VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL
Sbjct: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180

Query: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240
           RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI
Sbjct: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240

Query: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300
           KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY
Sbjct: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300

Query: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360
           TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS
Sbjct: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360

Query: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420
           KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE
Sbjct: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420

Query: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480
           AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE
Sbjct: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480

Query: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR 540
           RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR
Sbjct: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR 540

Query: 541 NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK
Sbjct: 541 NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 589

BLAST of Cucsa.306820.1 vs. NCBI nr
Match: gi|659084068|ref|XP_008442691.1| (PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At2g32630-like [Cucumis melo])

HSP 1 Score: 1046.2 bits (2704), Expect = 2.2e-302
Identity = 520/589 (88.29%), Postives = 555/589 (94.23%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           MS QAIA NIAKLILKSGLQPFKTTPSLLS  DSRV Q +LSDPN+PT+SCLRFF+FLR+
Sbjct: 1   MSNQAIATNIAKLILKSGLQPFKTTPSLLSKLDSRVTQSILSDPNVPTKSCLRFFNFLRR 60

Query: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120
           NPS KPDLPAHLIL  RLYRARKFAEMKNVLKFIVN GNLWSNVERIVSSIGGEFNEP  
Sbjct: 61  NPSCKPDLPAHLILVCRLYRARKFAEMKNVLKFIVNGGNLWSNVERIVSSIGGEFNEPKF 120

Query: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180
           VE FCDMLFRVYMD RMFDSSLEVFDYARK GFEI+ERSCFEFLLALKRSGNMELCVEFL
Sbjct: 121 VENFCDMLFRVYMDKRMFDSSLEVFDYARKNGFEIEERSCFEFLLALKRSGNMELCVEFL 180

Query: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240
           RQ+VDSGIEIRV SWTAVVDGLCKKGEVVRAKAL+DELVCKGFKP+VITYNTLLNGYIEI
Sbjct: 181 RQIVDSGIEIRVSSWTAVVDGLCKKGEVVRAKALVDELVCKGFKPNVITYNTLLNGYIEI 240

Query: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300
           KD GGVNEILSLMEK+VVDYNV TYTMLIEWYSR SKIEE+EKLFDEMLKKGIEPDVY+Y
Sbjct: 241 KDEGGVNEILSLMEKDVVDYNVATYTMLIEWYSRISKIEESEKLFDEMLKKGIEPDVYVY 300

Query: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360
           TS+INWNCKFGNMKRAFVLFDEMTERRLVPNA+TYGAL+NGACKAG+M+AAEMMVNDMQS
Sbjct: 301 TSLINWNCKFGNMKRAFVLFDEMTERRLVPNAFTYGALVNGACKAGQMEAAEMMVNDMQS 360

Query: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420
           KGVDVN+VIFNTL+DGYC KGMIDEALRLQNIMQQKGFEID FTCNIIASGFCR +RREE
Sbjct: 361 KGVDVNQVIFNTLIDGYCXKGMIDEALRLQNIMQQKGFEIDVFTCNIIASGFCRLDRREE 420

Query: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480
           A+RLLLTMEERGVAP+VVSFSILIDIY KE+NFAEA+RL K MEKKG+ PSVVTYN FI+
Sbjct: 421 ARRLLLTMEERGVAPDVVSFSILIDIYGKEENFAEAKRLLKEMEKKGEVPSVVTYNTFIQ 480

Query: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNR 540
           RYCKKGKMEEAYKLI+EM+ERGLMPDTYTYTSL++ E ASGNVDRALELFNEMPQ GLNR
Sbjct: 481 RYCKKGKMEEAYKLIDEMKERGLMPDTYTYTSLMNWECASGNVDRALELFNEMPQRGLNR 540

Query: 541 NVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           N VTYTV+ISGLSK GRADEAFKLYDEMN +GI+PDD I+SSLIASLH+
Sbjct: 541 NEVTYTVMISGLSKAGRADEAFKLYDEMNTQGIIPDDRIFSSLIASLHE 589

BLAST of Cucsa.306820.1 vs. NCBI nr
Match: gi|700203836|gb|KGN58969.1| (hypothetical protein Csa_3G740130 [Cucumis sativus])

HSP 1 Score: 915.6 bits (2365), Expect = 4.4e-263
Identity = 469/528 (88.83%), Postives = 481/528 (91.10%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ
Sbjct: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60

Query: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120
           NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI
Sbjct: 61  NPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPSI 120

Query: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180
           VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL
Sbjct: 121 VEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFL 180

Query: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240
           RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI
Sbjct: 181 RQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEI 240

Query: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300
           KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY
Sbjct: 241 KDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIY 300

Query: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360
           TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS
Sbjct: 301 TSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQS 360

Query: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420
           KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE
Sbjct: 361 KGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREE 420

Query: 421 AKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIE 480
           AKRLLLTMEERGVAPNVVSF    +      N   A  LF  M + G   +VVTY   I 
Sbjct: 421 AKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTVIIS 480

Query: 481 RYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALE 529
              K G+ +EA+KL +EM + G++PD   Y+SLI      G +   LE
Sbjct: 481 GLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHKVGPLVSGLE 524

BLAST of Cucsa.306820.1 vs. NCBI nr
Match: gi|700203836|gb|KGN58969.1| (hypothetical protein Csa_3G740130 [Cucumis sativus])

HSP 1 Score: 264.2 bits (674), Expect = 5.3e-67
Identity = 142/342 (41.52%), Postives = 215/342 (62.87%), Query Frame = 1

Query: 248 EILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWN 307
           E L  M  + ++  V ++T +++   +  ++  A+ L DE++ KG +P V  Y +++N  
Sbjct: 178 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 237

Query: 308 CKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNR 367
            +  ++     +   M +  +  N  TY  LI    ++ +++ AE + ++M  KG++ + 
Sbjct: 238 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 297

Query: 368 VIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLT 427
            I+ ++++  CK G +  A  L + M ++    +A+T   + +G C++   + A+ ++  
Sbjct: 298 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 357

Query: 428 MEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGK 487
           M+ +GV  N V F+ L+D YCK+    EA RL  +M++KG      T N     +C+  +
Sbjct: 358 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 417

Query: 488 MEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTV 547
            EEA +L+  M+ERG+ P+  ++    +GERASGNVDRALELFNEMPQLGLNRNVVTYTV
Sbjct: 418 REEAKRLLLTMEERGVAPNVVSF----NGERASGNVDRALELFNEMPQLGLNRNVVTYTV 477

Query: 548 IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 590
           IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK
Sbjct: 478 IISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLHK 515


HSP 2 Score: 148.7 bits (374), Expect = 3.3e-32
Identity = 85/310 (27.42%), Postives = 160/310 (51.61%), Query Frame = 1

Query: 267 MLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINWNCKFGNMKRAFVLFDEMTER 326
           ML   Y  +   + + ++FD   KKG E D       +    + GNM+       +M + 
Sbjct: 127 MLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEFLRQMVDS 186

Query: 327 RLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVNRVIFNTLMDGYCKKGMIDEA 386
            +     ++ A+++G CK GE+  A+ +++++  KG   + + +NTL++GY +   +   
Sbjct: 187 GIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIEIKDVGGV 246

Query: 387 LRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLLTMEERGVAPNVVSFSILIDI 446
             + ++M++   + +  T  ++   + RS++ EEA++L   M ++G+ P+V  ++ +I+ 
Sbjct: 247 NEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYIYTSIINW 306

Query: 447 YCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFIERYCKKGKMEEAYKLINEMQERGLMPD 506
            CK  N   A  LF  M ++   P+  TY A I   CK G+M+ A  ++N+MQ +G+  +
Sbjct: 307 NCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQSKGVDVN 366

Query: 507 TYTYTSLIDGERASGNVDRALELFNEMPQLGLNRNVVTYTVIISGLSKDGRADEAFKLYD 566
              + +L+DG    G +D AL L N M Q G   +  T  +I SG  +  R +EA +L  
Sbjct: 367 RVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRREEAKRLLL 426

Query: 567 EMNKEGIVPD 577
            M + G+ P+
Sbjct: 427 TMEERGVAPN 436


HSP 3 Score: 719.5 bits (1856), Expect = 4.6e-204
Identity = 359/591 (60.74%), Postives = 452/591 (76.48%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTTPSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLRQ 60
           +S Q IA  I+K ++ SGLQP K+TPSLL N +  +  LVLS+P +P  SCL  F+FL+ 
Sbjct: 21  ISNQEIAQKISKTLINSGLQPLKSTPSLLFNLNPHITNLVLSNPLVPPLSCLSLFNFLQT 80

Query: 61  NPS---RKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNE 120
           NPS    KP+L AH+ L  RLY+A+KFA+MK VL  IV++  L   V  IVS    E   
Sbjct: 81  NPSLTSHKPNLQAHVTLICRLYQAKKFAQMKIVLNGIVSNDILRCPVSEIVSLAEDERKG 140

Query: 121 PSIVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCV 180
              VE  CDMLFRVY DN+MF+ ++ VFDYA KKGFEI+ERSCF  LLALK+ G ++LC+
Sbjct: 141 VKFVETLCDMLFRVYADNKMFEEAVGVFDYAEKKGFEIEERSCFVLLLALKKCGQVDLCL 200

Query: 181 EFLRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGY 240
            F  QMV+ G++I V S T V++ LCK+GEV +AKALM E+  +  KP+V+TYNTLL  Y
Sbjct: 201 RFFDQMVEKGVQITVHSLTLVMNELCKRGEVKKAKALMGEMAGRQIKPNVVTYNTLLKAY 260

Query: 241 IEIKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDV 300
           IE KD  GVNE LSLMEK+ V YNV T T+LI+W+    KIE+AEK+F+EM ++GI+PDV
Sbjct: 261 IERKDYEGVNEALSLMEKDGVGYNVATCTLLIDWFGSCEKIEDAEKVFEEMHERGIDPDV 320

Query: 301 YIYTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVND 360
           YIYTSIINWN + GNMKRA  LFDE+T+R LVPN +TYGALINGA K G+MK A+++VN+
Sbjct: 321 YIYTSIINWNGRVGNMKRALFLFDELTQRGLVPNVHTYGALINGAYKGGQMKMADILVNE 380

Query: 361 MQSKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNR 420
           MQSKG+DVN+V+FNTL+DGYC+KGM+DE LRLQ+ M+ KGF+ D FT + IASG C+ NR
Sbjct: 381 MQSKGIDVNQVVFNTLIDGYCRKGMMDEVLRLQDFMEGKGFQTDVFTYSTIASGLCKLNR 440

Query: 421 REEAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNA 480
            EEAK LL TMEERGVAPN+V F+ LIDI+CKE NF EA+++ + ME+KG+ P+ VTYNA
Sbjct: 441 NEEAKSLLFTMEERGVAPNLVCFTTLIDIFCKEGNFVEAKQVIQEMERKGERPNTVTYNA 500

Query: 481 FIERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLG 540
            I+ + KKGKM+EA+KL  EM+++GLMPDTYTY+SLI GE     VD AL+LF+EM Q  
Sbjct: 501 LIDGHIKKGKMKEAHKLKKEMEDKGLMPDTYTYSSLIHGECIDAKVDEALKLFHEMYQRD 560

Query: 541 LNRNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
           L RNVVTYT +ISGLSKDGR DEAFKLYDEM +EG+ PDD +Y SL+ SLH
Sbjct: 561 LTRNVVTYTAMISGLSKDGRTDEAFKLYDEMKREGLTPDDRVYHSLVGSLH 611

BLAST of Cucsa.306820.1 vs. NCBI nr
Match: gi|694403797|ref|XP_009376813.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Pyrus x bretschneideri])

HSP 1 Score: 713.4 bits (1840), Expect = 3.3e-202
Identity = 352/589 (59.76%), Postives = 446/589 (75.72%), Query Frame = 1

Query: 1   MSKQAIAANIAKLILKSGLQPFKTT-PSLLSNFDSRVMQLVLSDPNLPTRSCLRFFDFLR 60
           +S Q  A  IAK ++KSGLQP K+T PSLLSN +  V  LVLS+P +P  SCL  F+FL+
Sbjct: 20  ISNQETAEKIAKTLIKSGLQPLKSTSPSLLSNLNPHVTNLVLSNPLVPPHSCLTLFNFLQ 79

Query: 61  QNPSRKPDLPAHLILFSRLYRARKFAEMKNVLKFIVNDGNLWSNVERIVSSIGGEFNEPS 120
            +PS KP+L AH IL  RLY AR+FA+MK VL  IV + NL   V +I   I  E +   
Sbjct: 80  TDPSHKPNLQAHAILIRRLYNARRFAQMKIVLNGIVTNDNLRCPVSKITCLIEDEPDGVK 139

Query: 121 IVEKFCDMLFRVYMDNRMFDSSLEVFDYARKKGFEIDERSCFEFLLALKRSGNMELCVEF 180
            V   CDMLFRVY DN+MF+ ++ VF+Y  K G EI+ERSCF  LLALK+ G ++LC+ F
Sbjct: 140 FVGTLCDMLFRVYADNKMFEEAIGVFEYVGKNGLEIEERSCFVLLLALKKCGRVDLCLRF 199

Query: 181 LRQMVDSGIEIRVCSWTAVVDGLCKKGEVVRAKALMDELVCKGFKPSVITYNTLLNGYIE 240
             QMV+ G+ I V   T V++ LCKKGEV +AKALM E   +G KP+V T+NTLL  YIE
Sbjct: 200 FDQMVEKGVRITVYPLTLVMNELCKKGEVEKAKALMRETADRGTKPNVFTFNTLLKAYIE 259

Query: 241 IKDVGGVNEILSLMEKNVVDYNVTTYTMLIEWYSRSSKIEEAEKLFDEMLKKGIEPDVYI 300
            KD  GVNE+L LMEK+ V YNV T T+LI+W+  S KIE+AEK+F+EM ++GI+PDVY+
Sbjct: 260 RKDFEGVNEVLRLMEKDGVGYNVATCTLLIDWFGSSEKIEDAEKVFEEMRERGIQPDVYV 319

Query: 301 YTSIINWNCKFGNMKRAFVLFDEMTERRLVPNAYTYGALINGACKAGEMKAAEMMVNDMQ 360
           YTSIINWNC+ GNMKRA  LFDE T+R  VPN +TYGALINGACKAG+++ A+++VN+MQ
Sbjct: 320 YTSIINWNCRAGNMKRALFLFDECTQRGFVPNDHTYGALINGACKAGQLEMADILVNEMQ 379

Query: 361 SKGVDVNRVIFNTLMDGYCKKGMIDEALRLQNIMQQKGFEIDAFTCNIIASGFCRSNRRE 420
            KG+DVN+V+FNTLMDGYC+ GM+D+ALRLQ++M+ KGF++D FT + IASG C+ NR E
Sbjct: 380 GKGIDVNQVVFNTLMDGYCRSGMMDQALRLQDVMENKGFQLDVFTYSTIASGLCKLNRNE 439

Query: 421 EAKRLLLTMEERGVAPNVVSFSILIDIYCKEQNFAEARRLFKVMEKKGKAPSVVTYNAFI 480
           EAK LL TM ERGVAPNVV F+ LIDI+CKE NF EA R+F+ ME+KG+ P+ VTYNA I
Sbjct: 440 EAKSLLFTMAERGVAPNVVCFTTLIDIFCKEGNFVEANRVFQDMERKGERPNTVTYNALI 499

Query: 481 ERYCKKGKMEEAYKLINEMQERGLMPDTYTYTSLIDGERASGNVDRALELFNEMPQLGLN 540
           + Y KKGKM+EA+KL  EM+++GLMPD YTY+SLI+G+   G VD AL+LF+EM +  + 
Sbjct: 500 DGYIKKGKMKEAHKLKEEMEDKGLMPDMYTYSSLINGKCIDGKVDEALKLFDEMSRRDIP 559

Query: 541 RNVVTYTVIISGLSKDGRADEAFKLYDEMNKEGIVPDDGIYSSLIASLH 589
            NVVTYT +ISGLSKDGR +EAFKLYDEM   G+ PDD +Y SL+ SLH
Sbjct: 560 HNVVTYTAMISGLSKDGRTEEAFKLYDEMKNAGLTPDDRVYHSLVGSLH 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP180_ARATH5.0e-17452.70Pentatricopeptide repeat-containing protein At2g32630 OS=Arabidopsis thaliana GN... [more]
PP407_ARATH3.6e-7132.61Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana GN... [more]
PPR27_ARATH7.4e-6930.62Pentatricopeptide repeat-containing protein At1g09820 OS=Arabidopsis thaliana GN... [more]
PP152_ARATH4.8e-6831.60Pentatricopeptide repeat-containing protein At2g15630, mitochondrial OS=Arabidop... [more]
PPR26_ARATH1.4e-6727.83Putative pentatricopeptide repeat-containing protein At1g09680 OS=Arabidopsis th... [more]
Match NameE-valueIdentityDescription
A0A0A0LDI0_CUCSA3.1e-26388.83Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1[more]
A0A0A0LDI0_CUCSA3.7e-6741.52Uncharacterized protein OS=Cucumis sativus GN=Csa_3G740130 PE=4 SV=1[more]
V4S3K8_9ROSI2.7e-19556.63Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011284mg PE=4 SV=1[more]
A0A067DGQ8_CITSI6.1e-19556.63Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g047794mg PE=4 SV=1[more]
A0A165WT71_DAUCA2.0e-19055.42Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_014992 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G32630.12.8e-17552.70 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT5G39710.12.0e-7232.61 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT1G09820.14.2e-7030.62 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT2G15630.12.7e-6931.60 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT1G09680.17.9e-6927.83 Pentatricopeptide repeat (PPR) superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449440401|ref|XP_004137973.1|0.0e+00100.00PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Cucumis sativu... [more]
gi|659084068|ref|XP_008442691.1|2.2e-30288.29PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At2g... [more]
gi|700203836|gb|KGN58969.1|4.4e-26388.83hypothetical protein Csa_3G740130 [Cucumis sativus][more]
gi|700203836|gb|KGN58969.1|5.3e-6741.52hypothetical protein Csa_3G740130 [Cucumis sativus][more]
gi|694403797|ref|XP_009376813.1|3.3e-20259.76PREDICTED: pentatricopeptide repeat-containing protein At2g32630 [Pyrus x bretsc... [more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.306820Cucsa.306820gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.306820.1Cucsa.306820.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.306820.1.CDS.1Cucsa.306820.1.CDS.1CDS


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 264..293
score: 4.5E-8coord: 166..188
score:
IPR002885Pentatricopeptide repeatPFAMPF12854PPR_1coord: 431..464
score: 6.
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 192..237
score: 9.8E-12coord: 541..588
score: 2.4E-14coord: 470..516
score: 1.5E-20coord: 295..344
score: 2.3E-15coord: 366..414
score: 9.5
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 438..471
score: 1.1E-6coord: 298..332
score: 1.6E-5coord: 543..576
score: 2.4E-10coord: 264..297
score: 1.3E-10coord: 473..507
score: 2.6E-10coord: 333..366
score: 1.7E-7coord: 194..227
score: 1.8E-6coord: 508..541
score: 4.5E-4coord: 368..402
score: 9.4E-9coord: 403..437
score: 1.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 471..505
score: 14.063coord: 506..540
score: 10.83coord: 261..295
score: 13.68coord: 296..330
score: 10.885coord: 366..400
score: 12.31coord: 191..225
score: 10.337coord: 331..365
score: 10.961coord: 121..155
score: 6.741coord: 436..470
score: 12.003coord: 156..190
score: 7.783coord: 401..435
score: 12.003coord: 226..256
score: 7.805coord: 541..575
score: 13
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 474..566
score: 7.6E-6coord: 261..313
score: 7.
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 41..71
score: 9.7E-221coord: 126..584
score: 9.7E
NoneNo IPR availablePANTHERPTHR24015:SF418SUBFAMILY NOT NAMEDcoord: 41..71
score: 9.7E-221coord: 126..584
score: 9.7E
NoneNo IPR availableunknownSSF81901HCP-likecoord: 376..567
score: 6.2