Cucsa.252480 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.252480
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionCa2+-binding protein 1
Locationscaffold02229 : 1411850 .. 1412317 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCCCTTCCGGTACCGCCTCCGACGATCGTTCTGACGTTGCGTCCGTCTGTTTCCGTCAGGCATTCGAGGTACTCGACGCCGACCACGACGGAAAGATTAGCCGCGACGATCTTCGTAAATTCTACAGCGGCGGCGGCGATGCCGACGAAGATGCAATTGGGTCAATGATAGCCGCTGCGGACTTGAATCGGAACGGAGTTGTAGAGTATGAGGAGTTCGAGCGCGTATTGTCCGGGGGACGGAGGAGATCGACGGGGATTATGGAGGAGGTGTTTAAGACGATGGATAAAGATGGAGATGGACGGTTGAGCCATGGCGACTTGAAGAGCTATATGCATTTGGCTGGATTTTCGATATCTGATGAAGAGGTAACGGCGATGATTAGGTTTGGCGGTGGAGATGAATCGGACGGCGTTTGTTATGAAGGATTGCTCAAGATTTTGGCCGTTGATAATATGTATTAA

mRNA sequence

ATGTGCCCTTCCGGTACCGCCTCCGACGATCGTTCTGACGTTGCGTCCGTCTGTTTCCGTCAGGCATTCGAGGTACTCGACGCCGACCACGACGGAAAGATTAGCCGCGACGATCTTCGTAAATTCTACAGCGGCGGCGGCGATGCCGACGAAGATGCAATTGGGTCAATGATAGCCGCTGCGGACTTGAATCGGAACGGAGTTGTAGAGTATGAGGAGTTCGAGCGCGTATTGTCCGGGGGACGGAGGAGATCGACGGGGATTATGGAGGAGGTGTTTAAGACGATGGATAAAGATGGAGATGGACGGTTGAGCCATGGCGACTTGAAGAGCTATATGCATTTGGCTGGATTTTCGATATCTGATGAAGAGGTAACGGCGATGATTAGGTTTGGCGGTGGAGATGAATCGGACGGCGTTTGTTATGAAGGATTGCTCAAGATTTTGGCCGTTGATAATATGTATTAA

Coding sequence (CDS)

ATGTGCCCTTCCGGTACCGCCTCCGACGATCGTTCTGACGTTGCGTCCGTCTGTTTCCGTCAGGCATTCGAGGTACTCGACGCCGACCACGACGGAAAGATTAGCCGCGACGATCTTCGTAAATTCTACAGCGGCGGCGGCGATGCCGACGAAGATGCAATTGGGTCAATGATAGCCGCTGCGGACTTGAATCGGAACGGAGTTGTAGAGTATGAGGAGTTCGAGCGCGTATTGTCCGGGGGACGGAGGAGATCGACGGGGATTATGGAGGAGGTGTTTAAGACGATGGATAAAGATGGAGATGGACGGTTGAGCCATGGCGACTTGAAGAGCTATATGCATTTGGCTGGATTTTCGATATCTGATGAAGAGGTAACGGCGATGATTAGGTTTGGCGGTGGAGATGAATCGGACGGCGTTTGTTATGAAGGATTGCTCAAGATTTTGGCCGTTGATAATATGTATTAA

Protein sequence

MCPSGTASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAAADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSISDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY*
Homology
[Close]
BLAST of Cucsa.252480 vs. Swiss-Prot
Match: CABO_DORPE (Squidulin OS=Doryteuthis pealeii PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-10
Identity = 45/136 (33.09%), Postives = 70/136 (51.47%), Query Frame = 1 

Query: 20  RQAFEVLDADHDGKISRDDLRKFYSGGGDADEDA-IGSMIAAADLNRNGVVEYEEFERVL 79
           + AF++ D D DG+I+  +LR      G    DA +  MI   D + NG +EY EF  ++
Sbjct: 13  KDAFDMFDIDGDGQITSKELRSVMKSLGRTPSDAELEEMIREVDTDGNGTIEYAEFVEMM 72

Query: 80  SG--GRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFS---ISDEEVTAMIRFGG 139
           +   G       M E F+  DKDG+G ++  +L+  M  A FS   ++ EE++ MIR   
Sbjct: 73  AKQMGPTDPEKEMREAFRVFDKDGNGLITAAELRQVM--ANFSDEKLTSEEISEMIREAD 132

Query: 140 GDESDGVCYEGLLKIL 150
            D    V YE  +K++
Sbjct: 133 IDGDGMVNYEEFVKMM 146

[Close]
BLAST of Cucsa.252480 vs. Swiss-Prot
Match: CML1_ORYSI (Calmodulin-like protein 1 OS=Oryza sativa subsp. indica GN=CML1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.5e-10
Identity = 46/145 (31.72%), Postives = 74/145 (51.03%), Query Frame = 1 

Query: 8   SDDRSDVASVCFRQAFEVLDADHDGKISRDDL-RKFYSGGGDADEDAIGSMIAAADLNRN 67
           +D  S+   V FR+AF + D D DG I+  +L     S G +  E  +  MI+  D + N
Sbjct: 2   ADQLSEEQIVEFREAFSLFDKDGDGSITTKELGTVMRSLGQNPTEAELQDMISEVDADSN 61

Query: 68  GVVEYEEFERVLSGGRR--RSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSISDEE 127
           G +E++EF  +++   R   S   ++E F+  DKD +G +S  +L+  M   G  ++DEE
Sbjct: 62  GNIEFKEFLGLMARKLRDKDSEEELKEAFRVFDKDQNGFISAAELRHVMANIGERLTDEE 121

Query: 128 VTAMIRFGGGDESDGVCYEGLLKIL 150
           V  MI     D    + YE  +K +
Sbjct: 122 VGEMISEADVDGDGQINYEEFVKCM 146


HSP 2 Score: 47.0 bits (110), Expect = 2.2e-04
Identity = 26/85 (30.59%), Postives = 47/85 (55.29%), Query Frame = 1 

Query: 20  RQAFEVLDADHDGKISRDDLRKFYSGGGDA-DEDAIGSMIAAADLNRNGVVEYEEFERVL 79
           ++AF V D D +G IS  +LR   +  G+   ++ +G MI+ AD++ +G + YEEF + +
Sbjct: 87  KEAFRVFDKDQNGFISAAELRHVMANIGERLTDEEVGEMISEADVDGDGQINYEEFVKCM 146

Query: 80  SGGRRRSTGIMEEVFKTMDKDGDGR 104
              +RR     + + +  + DG  R
Sbjct: 147 MAKKRR-----KRIEEKREHDGGSR 166

[Close]
BLAST of Cucsa.252480 vs. Swiss-Prot
Match: CALM_CANAX (Calmodulin OS=Candida albicans GN=CMD1 PE=3 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 2.3e-09
Identity = 36/115 (31.30%), Postives = 63/115 (54.78%), Query Frame = 1 

Query: 19  FRQAFEVLDADHDGKISRDDL-RKFYSGGGDADEDAIGSMIAAADLNRNGVVEYEEFERV 78
           F++AF + D D DGKI+  +L     S G +  E  +  MI   D+N +G +++ EF  +
Sbjct: 13  FKEAFSLFDKDSDGKITTKELGTVMRSLGQNPSESELTDMINEVDVNSDGSIDFPEFLTM 72

Query: 79  LSGGRRRSTGIME--EVFKTMDKDGDGRLSHGDLKSYMHLAGFSISDEEVTAMIR 131
           ++   + +    E  E FK  D++GDG++S  +L+  +   G  +SD +V  MI+
Sbjct: 73  MARKMKDTDSEAEIAEAFKVFDRNGDGKISAAELRHLLTSIGEKLSDADVDQMIK 127


HSP 2 Score: 43.5 bits (101), Expect = 2.4e-03
Identity = 25/73 (34.25%), Postives = 42/73 (57.53%), Query Frame = 1 

Query: 8   SDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDA-IGSMIAAADLNRN 67
           +D  +++A     +AF+V D + DGKIS  +LR   +  G+   DA +  MI  AD N +
Sbjct: 80  TDSEAEIA-----EAFKVFDRNGDGKISAAELRHLLTSIGEKLSDADVDQMIKEADTNND 139

Query: 68  GVVEYEEFERVLS 80
           G ++ +EF  +L+
Sbjct: 140 GEIDIQEFTSLLA 147

[Close]
BLAST of Cucsa.252480 vs. Swiss-Prot
Match: CALM_SCHPO (Calmodulin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=cam1 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.9e-09
Identity = 42/136 (30.88%), Postives = 69/136 (50.74%), Query Frame = 1 

Query: 19  FRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDA-IGSMIAAADLNRNGVVEYEEFERV 78
           FR+AF + D D DG I+ ++L       G +   A +  MI   D + NG +++ EF  +
Sbjct: 14  FREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTM 73

Query: 79  LSGGRRRSTGIMEEV---FKTMDKDGDGRLSHGDLKSYMHLAGFSISDEEVTAMIRFGGG 138
           ++  + + T   EEV   FK  DKDG+G ++  +L   +   G  +S EEV  MIR    
Sbjct: 74  MA-RKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADT 133

Query: 139 DESDGVCYEGLLKILA 151
           D    + YE   ++++
Sbjct: 134 DGDGVINYEEFSRVIS 148

[Close]
BLAST of Cucsa.252480 vs. Swiss-Prot
Match: CML6_ORYSJ (Putative calmodulin-like protein 6 OS=Oryza sativa subsp. japonica GN=CML6 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 6.7e-09
Identity = 36/137 (26.28%), Postives = 70/137 (51.09%), Query Frame = 1 

Query: 19  FRQAFEVLDADHDGKISRDDLRKFYSGGGDA-DEDAIGSMIAAADLNRNGVVEYEEF--- 78
           FR AF + D ++DG ISR++L    +  G A  ++ +  MI A D + NG +E++EF   
Sbjct: 13  FRDAFSLFDKNNDGCISREELATVLTRLGMAPSQEDLQDMIVAVDEDGNGTIEFDEFLAI 72

Query: 79  --ERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSISDEEVTAMIRFG 138
             +++    +      + + F+  DKD +G +S  +L   M   G  ++++E+  M++  
Sbjct: 73  MKKKLYENDKGDDEEELRKAFRIFDKDDNGFISRNELSMVMASLGEEMTEDEIDDMMKAA 132

Query: 139 GGDESDGVCYEGLLKIL 150
             +    V YE   +++
Sbjct: 133 DSNNDGQVDYEEFKRVM 149


HSP 2 Score: 54.7 bits (130), Expect = 1.1e-06
Identity = 32/76 (42.11%), Postives = 46/76 (60.53%), Query Frame = 1 

Query: 20  RQAFEVLDADHDGKISRDDLRKFYSG-GGDADEDAIGSMIAAADLNRNGVVEYEEFERVL 79
           R+AF + D D +G ISR++L    +  G +  ED I  M+ AAD N +G V+YEEF+RV+
Sbjct: 90  RKAFRIFDKDDNGFISRNELSMVMASLGEEMTEDEIDDMMKAADSNNDGQVDYEEFKRVM 149

Query: 80  SGGRRRSTGIMEEVFK 95
                 ST  + E+FK
Sbjct: 150 -----MSTWNITEIFK 160

[Close]
BLAST of Cucsa.252480 vs. TrEMBL
Match: A0A0A0L828_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G167380 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 2.0e-81
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 1 

Query: 1   MCPSGTASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA 60
           MCPSGTASDDRSDVASV FRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA
Sbjct: 22  MCPSGTASDDRSDVASVGFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA 81

Query: 61  ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI 120
           ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI
Sbjct: 82  ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI 141

Query: 121 SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY 156
           SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY
Sbjct: 142 SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY 176

[Close]
BLAST of Cucsa.252480 vs. TrEMBL
Match: A0A067K6N7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19015 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.6e-46
Identity = 100/159 (62.89%), Postives = 125/159 (78.62%), Query Frame = 1 

Query: 1   MCPSGT--ASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSG--GGDADEDAIGS 60
           MCPSG   AS+ ++  A + FR AF+VLDAD DGKISRDDLR FY+G   G AD+D IGS
Sbjct: 1   MCPSGRSLASNGKTTSARLDFRTAFDVLDADRDGKISRDDLRSFYTGFLSGGADDDVIGS 60

Query: 61  MIAAADLNRNGVVEYEEFERVLSG-GRRRST--GIMEEVFKTMDKDGDGRLSHGDLKSYM 120
           M++ AD N++G VEYEEFERVL+G G+R+ T  G+ME+VFK MDKDGDGRLSH DLKSYM
Sbjct: 61  MMSLADFNKDGFVEYEEFERVLNGNGKRKKTYNGVMEDVFKVMDKDGDGRLSHEDLKSYM 120

Query: 121 HLAGFSISDEEVTAMIRFGGGDESDGVCYEGLLKILAVD 153
             AGF ++DE+V AMI+ GGGD+ D V ++ LLKIL++D
Sbjct: 121 QWAGFDLADEDVKAMIKLGGGDDKDSVSFDDLLKILSLD 159

[Close]
BLAST of Cucsa.252480 vs. TrEMBL
Match: A0A061EP14_THECC (Ca2+-binding protein 1 OS=Theobroma cacao GN=TCM_021387 PE=4 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 5.2e-45
Identity = 101/165 (61.21%), Postives = 123/165 (74.55%), Query Frame = 1 

Query: 1   MCPSGTASDDRSDVASVC-FRQAFEVLDADHDGKISRDDLRKFYSG----GGDADEDAIG 60
           MCPS      +   +SV  FR AFEVLDAD DGKISR+DLR+FY+G     G  D++ IG
Sbjct: 1   MCPSERNLSRQEKTSSVSDFRSAFEVLDADRDGKISREDLRRFYAGFSNGTGFDDDEIIG 60

Query: 61  SMIAAADLNRNGVVEYEEFERVLSGGRRRST--------GIMEEVFKTMDKDGDGRLSHG 120
           +MI+ AD N++G VEYEEFERVL G  +RS+        G+ME+VFK MDKDGDGRLSH 
Sbjct: 61  TMISLADSNKDGFVEYEEFERVLGGCGKRSSSASAAGGFGVMEDVFKVMDKDGDGRLSHE 120

Query: 121 DLKSYMHLAGFSISDEEVTAMIRFGGGDESDGVCYEGLLKILAVD 153
           DLKSYM+ AGFS SDE++ AMIR GGGDE++GV ++GLLKILAVD
Sbjct: 121 DLKSYMNWAGFSASDEDIKAMIRLGGGDENEGVSFDGLLKILAVD 165

[Close]
BLAST of Cucsa.252480 vs. TrEMBL
Match: A0A067G974_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031051mg PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.6e-44
Identity = 95/165 (57.58%), Postives = 121/165 (73.33%), Query Frame = 1 

Query: 1   MCPSGTASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSG--GGDADEDAIGSMI 60
           MCP+GTA   R        + AF+V+DAD DGKISRDDLR FY+G    + D D IG+MI
Sbjct: 1   MCPTGTALRPRDAAKPSRLKPAFDVIDADRDGKISRDDLRAFYAGRRASEEDGDVIGTMI 60

Query: 61  AAADLNRNGVVEYEEFERVL------SGGRRRSTG---IMEEVFKTMDKDGDGRLSHGDL 120
           + AD N++G VEY+EFERVL      +  + +S G   +ME+VFK MDKDGDGRLSHGDL
Sbjct: 61  SVADFNKDGYVEYDEFERVLDLEYGKANSKSKSNGGGGLMEDVFKVMDKDGDGRLSHGDL 120

Query: 121 KSYMHLAGFSISDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNM 155
           KSYM+ AGF+ +D+++ AMIR GGGDE+DGV ++GLLKILAVD +
Sbjct: 121 KSYMNCAGFAATDDDIKAMIRLGGGDENDGVSFDGLLKILAVDTV 165

[Close]
BLAST of Cucsa.252480 vs. TrEMBL
Match: A0A0D2T0I1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G257800 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.6e-44
Identity = 102/162 (62.96%), Postives = 118/162 (72.84%), Query Frame = 1 

Query: 1   MCPSGT---ASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDA---I 60
           MCPS       ++R       FR AFEVLDAD DGKISRDDLR+FY+G     EDA   I
Sbjct: 1   MCPSERNLRRPENRVPTPETDFRSAFEVLDADRDGKISRDDLRRFYAGFSYGSEDADEMI 60

Query: 61  GSMIAAADLNRNGVVEYEEFERVLSGGRRR----STGIMEEVFKTMDKDGDGRLSHGDLK 120
           G+MI+ ADLN +G VEY+EFERVL G   R    S G+ME+VFK MDKDGDGRLSH DLK
Sbjct: 61  GTMISLADLNSDGFVEYDEFERVLGGYNNRKKVSSYGVMEDVFKVMDKDGDGRLSHEDLK 120

Query: 121 SYMHLAGFSISDEEVTAMIRFGGGDESDGVCYEGLLKILAVD 153
           SYM+ AGFS SDE++ AMIR GGGD+  GVC++GLLKILAVD
Sbjct: 121 SYMNWAGFSASDEDIKAMIRLGGGDDK-GVCFDGLLKILAVD 161

[Close]
BLAST of Cucsa.252480 vs. TAIR10
Match: AT5G49480.1 (AT5G49480.1 Ca2+-binding protein 1)

HSP 1 Score: 169.1 bits (427), Expect = 2.2e-42
Identity = 85/157 (54.14%), Postives = 112/157 (71.34%), Query Frame = 1 

Query: 1   MCPSGTASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSG---GGDADEDAIGSM 60
           MCPSG  +   +  A+  FR AFE++D DHDGKIS DDLR FY+G   G + DE  IG+M
Sbjct: 1   MCPSGRIAIPITTTANPNFRPAFEIIDTDHDGKISSDDLRAFYAGIPSGENNDETMIGTM 60

Query: 61  IAAADLNRNGVVEYEEFERVLS------GGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKS 120
           I+ AD N++G VE++EFE+VL        G     G+M++VFK MDKDGDGRLS+GDLKS
Sbjct: 61  ISVADANKDGFVEFDEFEKVLETTPFSRSGNGGDDGLMKDVFKVMDKDGDGRLSYGDLKS 120

Query: 121 YMHLAGFSISDEEVTAMIRFGGGDESDGVCYEGLLKI 149
           YM  AG +++D+E+ +MIR  GGD +DGV ++GLLKI
Sbjct: 121 YMDSAGLAVTDDEIKSMIRLAGGDLNDGVSFDGLLKI 157

[Close]
BLAST of Cucsa.252480 vs. TAIR10
Match: AT3G51920.1 (AT3G51920.1 calmodulin 9)

HSP 1 Score: 57.0 bits (136), Expect = 1.2e-08
Identity = 35/115 (30.43%), Postives = 62/115 (53.91%), Query Frame = 1 

Query: 19  FRQAFEVLDADHDGKISRDDLRK-FYSGGGDADEDAIGSMIAAADLNRNGVVEYEEFERV 78
           F +AF ++D D DG I+++ L K   S G +   + +  M++  D+  NG + +++F  +
Sbjct: 13  FYEAFCLIDKDSDGFITKEKLTKVMKSMGKNPKAEQLQQMMSDVDIFGNGGITFDDFLYI 72

Query: 79  LSGGRRRSTGIME--EVFKTMDKDGDGRLSHGDLKSYMHLAGFSISDEEVTAMIR 131
           ++    + +   E  EVF+  D+DGDG +S  +L   M   G  I+ EE   M+R
Sbjct: 73  MAQNTSQESASDELIEVFRVFDRDGDGLISQLELGEGMKDMGMKITAEEAEHMVR 127

[Close]
BLAST of Cucsa.252480 vs. TAIR10
Match: AT3G10190.1 (AT3G10190.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-07
Identity = 42/128 (32.81%), Postives = 62/128 (48.44%), Query Frame = 1 

Query: 3   PSGTASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYS--GGGDADEDAIGSMIAA 62
           P+    +  S  + V   QAF+++D D+DG +SR DL    S  G     E+ I  M+  
Sbjct: 55  PTSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKE 114

Query: 63  ADLNRNGVVEYEEF-ERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFS 122
            D + +G +  EE   RV+S    R +  ++E F+  D D DG +S  +L     L  FS
Sbjct: 115 VDCDGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADEL-----LRVFS 174

Query: 123 -ISDEEVT 127
            I DE  T
Sbjct: 175 TIGDERCT 177


HSP 2 Score: 40.8 bits (94), Expect = 8.9e-04
Identity = 24/61 (39.34%), Postives = 34/61 (55.74%), Query Frame = 1 

Query: 20  RQAFEVLDADHDGKISRDDLRKFYSGGGD--ADEDAIGSMIAAADLNRNGVVEYEEFERV 79
           ++ FE  DAD DG IS D+L + +S  GD     D    MIA  D + +G V + EF R+
Sbjct: 145 KETFEFFDADRDGLISADELLRVFSTIGDERCTLDDCKRMIADVDEDGDGFVCFTEFSRM 204

[Close]
BLAST of Cucsa.252480 vs. TAIR10
Match: AT4G12860.1 (AT4G12860.1 EF hand calcium-binding protein family)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-07
Identity = 32/111 (28.83%), Postives = 60/111 (54.05%), Query Frame = 1 

Query: 10  DRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSGGGD-ADEDAIGSMIAAADLNRNGV 69
           DR +++ V     F++ D + DGKI++++L+ F+   G    E+ I  MIA  D+N +G 
Sbjct: 2   DRGELSRV-----FQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGA 61

Query: 70  VEYEEFERVLSG--GRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAG 118
           ++ +EF  +       +     M E F+  D++GDG ++  +L+S +   G
Sbjct: 62  MDIDEFGSLYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMG 107


HSP 2 Score: 37.4 bits (85), Expect = 9.9e-03
Identity = 14/43 (32.56%), Postives = 25/43 (58.14%), Query Frame = 1 

Query: 87  GIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSISDEEVTAMI 130
           G +  VF+  DK+GDG+++  +LK +    G  + + E+  MI
Sbjct: 4   GELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMI 46

[Close]
BLAST of Cucsa.252480 vs. TAIR10
Match: AT1G76040.2 (AT1G76040.2 calcium-dependent protein kinase 29)

HSP 1 Score: 50.8 bits (120), Expect = 8.6e-07
Identity = 36/132 (27.27%), Postives = 61/132 (46.21%), Query Frame = 1 

Query: 20  RQAFEVLDADHDGKISRDDLRK-FYSGGGDADEDAIGSMIAAADLNRNGVVEYEEF-ERV 79
           +Q F+ +D D  G I+ D+LR   +  G    E  I  ++ AAD++++G ++Y EF    
Sbjct: 418 KQTFKNMDTDESGTITFDELRNGLHRLGSKLTESEIKQLMEAADVDKSGTIDYIEFVTAT 477

Query: 80  LSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSISDEEVTAMIRFGGGDES 139
           +   R      + E FK  DKD  G ++  +LK  M   G    D  +  +I     D  
Sbjct: 478 MHRHRLEKEENLIEAFKYFDKDRSGFITRDELKHSMTEYGMG-DDATIDEVINDVDTDND 537

Query: 140 DGVCYEGLLKIL 150
             + YE  + ++
Sbjct: 538 GRINYEEFVAMM 548

[Close]
BLAST of Cucsa.252480 vs. NCBI nr
Match: gi|449432924|ref|XP_004134248.1| (PREDICTED: calmodulin-A [Cucumis sativus])

HSP 1 Score: 309.7 bits (792), Expect = 2.9e-81
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 1 

Query: 1   MCPSGTASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA 60
           MCPSGTASDDRSDVASV FRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA
Sbjct: 1   MCPSGTASDDRSDVASVGFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA 60

Query: 61  ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI 120
           ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI
Sbjct: 61  ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI 120

Query: 121 SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY 156
           SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY
Sbjct: 121 SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY 155

[Close]
BLAST of Cucsa.252480 vs. NCBI nr
Match: gi|700202041|gb|KGN57174.1| (hypothetical protein Csa_3G167380 [Cucumis sativus])

HSP 1 Score: 309.7 bits (792), Expect = 2.9e-81
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 1 

Query: 1   MCPSGTASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA 60
           MCPSGTASDDRSDVASV FRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA
Sbjct: 22  MCPSGTASDDRSDVASVGFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA 81

Query: 61  ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI 120
           ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI
Sbjct: 82  ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI 141

Query: 121 SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY 156
           SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY
Sbjct: 142 SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY 176

[Close]
BLAST of Cucsa.252480 vs. NCBI nr
Match: gi|659076972|ref|XP_008438964.1| (PREDICTED: calmodulin-related protein [Cucumis melo])

HSP 1 Score: 300.1 bits (767), Expect = 2.3e-78
Identity = 151/155 (97.42%), Postives = 152/155 (98.06%), Query Frame = 1 

Query: 1   MCPSGTASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA 60
           MCPSGTASD RSDVASV FRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA
Sbjct: 1   MCPSGTASD-RSDVASVGFRQAFEVLDADHDGKISRDDLRKFYSGGGDADEDAIGSMIAA 60

Query: 61  ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHGDLKSYMHLAGFSI 120
           ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSH DLKSYMHLAGFSI
Sbjct: 61  ADLNRNGVVEYEEFERVLSGGRRRSTGIMEEVFKTMDKDGDGRLSHADLKSYMHLAGFSI 120

Query: 121 SDEEVTAMIRFGGGDESDGVCYEGLLKILAVDNMY 156
           SDEEVTAMIRFGGGDESDGVCYEGLLKILA+DNMY
Sbjct: 121 SDEEVTAMIRFGGGDESDGVCYEGLLKILAIDNMY 154

[Close]
BLAST of Cucsa.252480 vs. NCBI nr
Match: gi|802704833|ref|XP_012084098.1| (PREDICTED: calmodulin [Jatropha curcas])

HSP 1 Score: 193.7 bits (491), Expect = 2.3e-46
Identity = 100/159 (62.89%), Postives = 125/159 (78.62%), Query Frame = 1 

Query: 1   MCPSGT--ASDDRSDVASVCFRQAFEVLDADHDGKISRDDLRKFYSG--GGDADEDAIGS 60
           MCPSG   AS+ ++  A + FR AF+VLDAD DGKISRDDLR FY+G   G AD+D IGS
Sbjct: 1   MCPSGRSLASNGKTTSARLDFRTAFDVLDADRDGKISRDDLRSFYTGFLSGGADDDVIGS 60

Query: 61  MIAAADLNRNGVVEYEEFERVLSG-GRRRST--GIMEEVFKTMDKDGDGRLSHGDLKSYM 120
           M++ AD N++G VEYEEFERVL+G G+R+ T  G+ME+VFK MDKDGDGRLSH DLKSYM
Sbjct: 61  MMSLADFNKDGFVEYEEFERVLNGNGKRKKTYNGVMEDVFKVMDKDGDGRLSHEDLKSYM 120

Query: 121 HLAGFSISDEEVTAMIRFGGGDESDGVCYEGLLKILAVD 153
             AGF ++DE+V AMI+ GGGD+ D V ++ LLKIL++D
Sbjct: 121 QWAGFDLADEDVKAMIKLGGGDDKDSVSFDDLLKILSLD 159

[Close]
BLAST of Cucsa.252480 vs. NCBI nr
Match: gi|590662006|ref|XP_007035829.1| (Ca2+-binding protein 1 [Theobroma cacao])

HSP 1 Score: 188.7 bits (478), Expect = 7.5e-45
Identity = 101/165 (61.21%), Postives = 123/165 (74.55%), Query Frame = 1 

Query: 1   MCPSGTASDDRSDVASVC-FRQAFEVLDADHDGKISRDDLRKFYSG----GGDADEDAIG 60
           MCPS      +   +SV  FR AFEVLDAD DGKISR+DLR+FY+G     G  D++ IG
Sbjct: 1   MCPSERNLSRQEKTSSVSDFRSAFEVLDADRDGKISREDLRRFYAGFSNGTGFDDDEIIG 60

Query: 61  SMIAAADLNRNGVVEYEEFERVLSGGRRRST--------GIMEEVFKTMDKDGDGRLSHG 120
           +MI+ AD N++G VEYEEFERVL G  +RS+        G+ME+VFK MDKDGDGRLSH 
Sbjct: 61  TMISLADSNKDGFVEYEEFERVLGGCGKRSSSASAAGGFGVMEDVFKVMDKDGDGRLSHE 120

Query: 121 DLKSYMHLAGFSISDEEVTAMIRFGGGDESDGVCYEGLLKILAVD 153
           DLKSYM+ AGFS SDE++ AMIR GGGDE++GV ++GLLKILAVD
Sbjct: 121 DLKSYMNWAGFSASDEDIKAMIRLGGGDENEGVSFDGLLKILAVD 165

The following BLAST results are available for this feature:
BLAST of Cucsa.252480 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CABO_DORPE1.6e-1033.09Squidulin OS=Doryteuthis pealeii PE=1 SV=1[more]
CML1_ORYSI3.5e-1031.72Calmodulin-like protein 1 OS=Oryza sativa subsp. indica GN=CML1 PE=1 SV=1[more]
CALM_CANAX2.3e-0931.30Calmodulin OS=Candida albicans GN=CMD1 PE=3 SV=2[more]
CALM_SCHPO3.9e-0930.88Calmodulin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=cam1 PE=1 S... [more]
CML6_ORYSJ6.7e-0926.28Putative calmodulin-like protein 6 OS=Oryza sativa subsp. japonica GN=CML6 PE=3 ... [more]
BLAST of Cucsa.252480 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L828_CUCSA2.0e-8199.35Uncharacterized protein OS=Cucumis sativus GN=Csa_3G167380 PE=4 SV=1[more]
A0A067K6N7_JATCU1.6e-4662.89Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19015 PE=4 SV=1[more]
A0A061EP14_THECC5.2e-4561.21Ca2+-binding protein 1 OS=Theobroma cacao GN=TCM_021387 PE=4 SV=1[more]
A0A067G974_CITSI2.6e-4457.58Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031051mg PE=4 SV=1[more]
A0A0D2T0I1_GOSRA2.6e-4462.96Uncharacterized protein OS=Gossypium raimondii GN=B456_006G257800 PE=4 SV=1[more]
BLAST of Cucsa.252480 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G49480.12.2e-4254.14 Ca2+-binding protein 1[more]
AT3G51920.11.2e-0830.43 calmodulin 9[more]
AT3G10190.11.3e-0732.81 Calcium-binding EF-hand family protein[more]
AT4G12860.11.3e-0728.83 EF hand calcium-binding protein family[more]
AT1G76040.28.6e-0727.27 calcium-dependent protein kinase 29[more]
BLAST of Cucsa.252480 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449432924|ref|XP_004134248.1|2.9e-8199.35PREDICTED: calmodulin-A [Cucumis sativus][more]
gi|700202041|gb|KGN57174.1|2.9e-8199.35hypothetical protein Csa_3G167380 [Cucumis sativus][more]
gi|659076972|ref|XP_008438964.1|2.3e-7897.42PREDICTED: calmodulin-related protein [Cucumis melo][more]
gi|802704833|ref|XP_012084098.1|2.3e-4662.89PREDICTED: calmodulin [Jatropha curcas][more]
gi|590662006|ref|XP_007035829.1|7.5e-4561.21Ca2+-binding protein 1 [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.252480.1Cucsa.252480.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 90..111
score: 3.
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 20..76
score: 1.0
IPR002048EF-hand domainSMARTSM00054efh_1coord: 88..116
score: 0.038coord: 53..81
score: 1.1coord: 18..46
score: 0
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 50..83
score: 6.647coord: 84..119
score: 12.756coord: 14..49
score: 12
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 19..150
score: 8.5
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 18..150
score: 4.7
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 97..109
score: -coord: 62..74
score: -coord: 27..39
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 19..155
score: 3.1
NoneNo IPR availablePANTHERPTHR10891:SF629SUBFAMILY NOT NAMEDcoord: 19..155
score: 3.1

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.252480Cla021803Watermelon (97103) v1cgywmB479
Cucsa.252480Csa3G167380Cucumber (Chinese Long) v2cgycuB366
Cucsa.252480MELO3C006721Melon (DHL92) v3.5.1cgymeB439
Cucsa.252480ClCG05G006630Watermelon (Charleston Gray)cgywcgB451
Cucsa.252480CSPI03G15050Wild cucumber (PI 183967)cgycpiB383
Cucsa.252480CmaCh14G015850Cucurbita maxima (Rimu)cgycmaB0709
Cucsa.252480CmaCh06G015660Cucurbita maxima (Rimu)cgycmaB0727
Cucsa.252480CmoCh06G015620Cucurbita moschata (Rifu)cgycmoB0718
Cucsa.252480Lsi05G014160Bottle gourd (USVL1VR-Ls)cgylsiB416
Cucsa.252480Cp4.1LG03g11850Cucurbita pepo (Zucchini)cgycpeB0682
Cucsa.252480Cp4.1LG08g05340Cucurbita pepo (Zucchini)cgycpeB0687
Cucsa.252480MELO3C006721.2Melon (DHL92) v3.6.1cgymedB432
Cucsa.252480CsaV3_3G015240Cucumber (Chinese Long) v3cgycucB387
Cucsa.252480Cla97C05G088220Watermelon (97103) v2cgywmbB452
Cucsa.252480Bhi01G000788Wax gourdcgywgoB522
Cucsa.252480Carg19616Silver-seed gourdcarcgyB0329
Cucsa.252480Carg23731Silver-seed gourdcarcgyB1072
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.252480Cucurbita pepo (Zucchini)cgycpeB0671
Cucsa.252480Cucurbita pepo (Zucchini)cgycpeB0678
Cucsa.252480Bottle gourd (USVL1VR-Ls)cgylsiB412
Cucsa.252480Melon (DHL92) v3.6.1cgymedB425
Cucsa.252480Melon (DHL92) v3.6.1cgymedB427
Cucsa.252480Silver-seed gourdcarcgyB0083
Cucsa.252480Silver-seed gourdcarcgyB0788
Cucsa.252480Watermelon (97103) v2cgywmbB453
Cucsa.252480Wax gourdcgywgoB512
Cucsa.252480Cucurbita maxima (Rimu)cgycmaB0714
Cucsa.252480Cucurbita maxima (Rimu)cgycmaB0731
Cucsa.252480Cucurbita moschata (Rifu)cgycmoB0700
Cucsa.252480Cucurbita moschata (Rifu)cgycmoB0707
Cucsa.252480Cucurbita moschata (Rifu)cgycmoB0720
Cucsa.252480Melon (DHL92) v3.5.1cgymeB430
Cucsa.252480Watermelon (Charleston Gray)cgywcgB453
Cucsa.252480Watermelon (97103) v1cgywmB483