Cucsa.123720 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.123720
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Unknown protein
Location	scaffold01000 : 824425 .. 825011 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCCATTTTCAACAACAAATCAAATCAAATCCAACTCCCCAATTTCCCAAACCACAATCAACTACAGAACCATGTCGTCGATGTTCCGTCCAAAAACGAAATCGAATTCACCGCCCGTCTCGTCGGAAAGCGCCTACTCTGCCAAAGAACTGAGACAGATCTTTAACCAAATGGATACGAATGAAGACGGAGGATTAGGATTGTTCGAGATCAAGAACGCATTGAAGAAACTGGACGCTGAAATGCCAGTTCATCTCGCTTACAGTGCTCTGTGTCACGCAGATCGAAACAGAGATGGAAAGCTTGGCGAGGAAGAGATCGATCAGTTCGTAAAGTACATGATGAGCAAAGGTTATGTTGTCTGATTTTTATTTACTGCCCCGCCGCCTCCCACGACGGCTTGTTGATCATATCGTCGGTTTCTATAAACTTGTCCAATAAATGGCGGCTGCTTCTTATGTGTATTTTTCTTTTATTTTACTTTCGAACCTCTGTTGCCTTTAATTTATTGACAATATATATCTCAATCAGTTGTATTTTAATTGTATATATCTTCAGTTCGTATGTGTTGTTATGTTTAGCTAAT

mRNA sequence

TTTCCATTTTCAACAACAAATCAAATCAAATCCAACTCCCCAATTTCCCAAACCACAATCAACTACAGAACCATGTCGTCGATGTTCCGTCCAAAAACGAAATCGAATTCACCGCCCGTCTCGTCGGAAAGCGCCTACTCTGCCAAAGAACTGAGACAGATCTTTAACCAAATGGATACGAATGAAGACGGAGGATTAGGATTGTTCGAGATCAAGAACGCATTGAAGAAACTGGACGCTGAAATGCCAGTTCATCTCGCTTACAGTGCTCTGTGTCACGCAGATCGAAACAGAGATGGAAAGCTTGGCGAGGAAGAGATCGATCAGTTCGTAAAGTACATGATGAGCAAAGGTTATGTTGTCTGATTTTTATTTACTGCCCCGCCGCCTCCCACGACGGCTTGTTGATCATATCGTCGGTTTCTATAAACTTGTCCAATAAATGGCGGCTGCTTCTTATGTGTATTTTTCTTTTATTTTACTTTCGAACCTCTGTTGCCTTTAATTTATTGACAATATATATCTCAATCAGTTGTATTTTAATTGTATATATCTTCAGTTCGTATGTGTTGTTATGTTTAGCTAAT

Coding sequence (CDS)

ATGTCGTCGATGTTCCGTCCAAAAACGAAATCGAATTCACCGCCCGTCTCGTCGGAAAGCGCCTACTCTGCCAAAGAACTGAGACAGATCTTTAACCAAATGGATACGAATGAAGACGGAGGATTAGGATTGTTCGAGATCAAGAACGCATTGAAGAAACTGGACGCTGAAATGCCAGTTCATCTCGCTTACAGTGCTCTGTGTCACGCAGATCGAAACAGAGATGGAAAGCTTGGCGAGGAAGAGATCGATCAGTTCGTAAAGTACATGATGAGCAAAGGTTATGTTGTCTGA

Protein sequence

MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV*

Homology

[Close]

BLAST of Cucsa.123720 vs. TrEMBL
Match: A0A0A0L3J7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639750 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 1.2e-47
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 1


Query: 1  MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV 60
          MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV
Sbjct: 1  MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV 60

Query: 61 HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
          HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV
Sbjct: 61 HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of Cucsa.123720 vs. TrEMBL
Match: W9S022_9ROSA (Putative calcium-binding protein CML10 OS=Morus notabilis GN=L484_000100 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.3e-09
Identity = 36/90 (40.00%), Postives = 51/90 (56.67%), Query Frame = 1


Query: 8   KTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSAL 67
           K+  N  P    + Y  ++L+ +  + DT+ DG L   E+K A K+L A  P   A  AL
Sbjct: 13  KSVQNHSP--KPAVYDKEKLKSVIRRYDTDGDGNLSRKELKKAFKELGATAPGWRAIRAL 72

Query: 68  CHADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
           CHAD+NRDG + EEE+   V Y   +GYV+
Sbjct: 73  CHADKNRDGFISEEELANLVHYAAQRGYVL 100

[Close]

BLAST of Cucsa.123720 vs. TrEMBL
Match: A0A061FBR4_THECC (Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_033849 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.1e-08
Identity = 31/71 (43.66%), Postives = 46/71 (64.79%), Query Frame = 1


Query: 26  ELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCHADRNRDGKLGEEEIDQ 85
           ++R IFN+ D N+DG L   E+K+A   L + MP   A+ AL HAD N DG + E+E ++
Sbjct: 46  QVRGIFNRFDANKDGRLSKQELKDAFASLGSRMPGFRAWLALQHADANGDGYISEDEFEK 105

Query: 86  FVKYMMSKGYV 97
            VKY + +GY+
Sbjct: 106 LVKYTLKRGYI 116

[Close]

BLAST of Cucsa.123720 vs. TrEMBL
Match: A0A061EED0_THECC (Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_017140 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.1e-08
Identity = 32/86 (37.21%), Postives = 50/86 (58.14%), Query Frame = 1


Query: 10  KSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCH 69
           + N PP  +E+     EL  +F + D + DG L   ++KNA   + +  P + A +ALC 
Sbjct: 35  RKNFPPALTEA-----ELISVFRRFDGDRDGRLSWQDLKNAFHSIGSRFPSYRALTALCQ 94

Query: 70  ADRNRDGKLGEEEIDQFVKYMMSKGY 96
           AD+NRDG + E+E+D  ++Y  S GY
Sbjct: 95  ADKNRDGYISEDEMDDLIQYAFSCGY 115

[Close]

BLAST of Cucsa.123720 vs. TrEMBL
Match: A0A0D2R5B7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G131800 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.4e-08
Identity = 37/86 (43.02%), Postives = 50/86 (58.14%), Query Frame = 1


Query: 10  KSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCH 69
           K  S P++ E      ++R IF++ DTN DG L   E+KNA   L + MP   A+ AL H
Sbjct: 118 KRASVPITEE------QVRGIFHRYDTNRDGLLSKQELKNAFSSLGSRMPGVRAWLALQH 177

Query: 70  ADRNRDGKLGEEEIDQFVKYMMSKGY 96
           AD N DG + E E D+ VKY + +GY
Sbjct: 178 ADVNGDGYINEAEFDKLVKYTLKRGY 197

[Close]

BLAST of Cucsa.123720 vs. NCBI nr
Match: gi|700200029|gb|KGN55187.1| (hypothetical protein Csa_4G639750 [Cucumis sativus])

HSP 1 Score: 196.8 bits (499), Expect = 1.7e-47
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 1


Query: 1  MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV 60
          MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV
Sbjct: 1  MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV 60

Query: 61 HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
          HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV
Sbjct: 61 HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of Cucsa.123720 vs. NCBI nr
Match: gi|703145680|ref|XP_010108597.1| (putative calcium-binding protein CML10 [Morus notabilis])

HSP 1 Score: 70.5 bits (171), Expect = 1.9e-09
Identity = 36/90 (40.00%), Postives = 51/90 (56.67%), Query Frame = 1


Query: 8   KTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSAL 67
           K+  N  P    + Y  ++L+ +  + DT+ DG L   E+K A K+L A  P   A  AL
Sbjct: 13  KSVQNHSP--KPAVYDKEKLKSVIRRYDTDGDGNLSRKELKKAFKELGATAPGWRAIRAL 72

Query: 68  CHADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
           CHAD+NRDG + EEE+   V Y   +GYV+
Sbjct: 73  CHADKNRDGFISEEELANLVHYAAQRGYVL 100

[Close]

BLAST of Cucsa.123720 vs. NCBI nr
Match: gi|590647148|ref|XP_007031821.1| (Calcium-binding EF-hand family protein, putative [Theobroma cacao])

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-08
Identity = 32/86 (37.21%), Postives = 50/86 (58.14%), Query Frame = 1


Query: 10  KSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCH 69
           + N PP  +E+     EL  +F + D + DG L   ++KNA   + +  P + A +ALC 
Sbjct: 35  RKNFPPALTEA-----ELISVFRRFDGDRDGRLSWQDLKNAFHSIGSRFPSYRALTALCQ 94

Query: 70  ADRNRDGKLGEEEIDQFVKYMMSKGY 96
           AD+NRDG + E+E+D  ++Y  S GY
Sbjct: 95  ADKNRDGYISEDEMDDLIQYAFSCGY 115

[Close]

BLAST of Cucsa.123720 vs. NCBI nr
Match: gi|590592354|ref|XP_007017256.1| (Calcium-binding EF-hand family protein, putative [Theobroma cacao])

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-08
Identity = 31/71 (43.66%), Postives = 46/71 (64.79%), Query Frame = 1


Query: 26  ELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCHADRNRDGKLGEEEIDQ 85
           ++R IFN+ D N+DG L   E+K+A   L + MP   A+ AL HAD N DG + E+E ++
Sbjct: 46  QVRGIFNRFDANKDGRLSKQELKDAFASLGSRMPGFRAWLALQHADANGDGYISEDEFEK 105

Query: 86  FVKYMMSKGYV 97
            VKY + +GY+
Sbjct: 106 LVKYTLKRGYI 116

[Close]

BLAST of Cucsa.123720 vs. NCBI nr
Match: gi|763747131|gb|KJB14570.1| (hypothetical protein B456_002G131800 [Gossypium raimondii])

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-08
Identity = 37/86 (43.02%), Postives = 50/86 (58.14%), Query Frame = 1


Query: 10  KSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCH 69
           K  S P++ E      ++R IF++ DTN DG L   E+KNA   L + MP   A+ AL H
Sbjct: 118 KRASVPITEE------QVRGIFHRYDTNRDGLLSKQELKNAFSSLGSRMPGVRAWLALQH 177

Query: 70  ADRNRDGKLGEEEIDQFVKYMMSKGY 96
           AD N DG + E E D+ VKY + +GY
Sbjct: 178 ADVNGDGYINEAEFDKLVKYTLKRGY 197

The following BLAST results are available for this feature:

BLAST of Cucsa.123720 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3J7_CUCSA	1.2e-47	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639750 PE=4 SV=1	[more]
W9S022_9ROSA	1.3e-09	40.00	Putative calcium-binding protein CML10 OS=Morus notabilis GN=L484_000100 PE=4 SV...	[more]
A0A061FBR4_THECC	1.1e-08	43.66	Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_03384...	[more]
A0A061EED0_THECC	1.1e-08	37.21	Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_01714...	[more]
A0A0D2R5B7_GOSRA	1.4e-08	43.02	Uncharacterized protein OS=Gossypium raimondii GN=B456_002G131800 PE=4 SV=1	[more]

BLAST of Cucsa.123720 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700200029\|gb\|KGN55187.1\|	1.7e-47	100.00	hypothetical protein Csa_4G639750 [Cucumis sativus]	[more]
gi\|703145680\|ref\|XP_010108597.1\|	1.9e-09	40.00	putative calcium-binding protein CML10 [Morus notabilis]	[more]
gi\|590647148\|ref\|XP_007031821.1\|	1.6e-08	37.21	Calcium-binding EF-hand family protein, putative [Theobroma cacao]	[more]
gi\|590592354\|ref\|XP_007017256.1\|	1.6e-08	43.66	Calcium-binding EF-hand family protein, putative [Theobroma cacao]	[more]
gi\|763747131\|gb\|KJB14570.1\|	2.1e-08	43.02	hypothetical protein B456_002G131800 [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.123720.1	Cucsa.123720.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 26..54 score: 3.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 62..90 score: 16.0coord: 26..54 score: 0
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 22..57 score: 11.529coord: 58..93 score: 8
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 17..90 score: 8.4
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 24..91 score: 1.13
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 71..83 scor

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.123720	Cla020680	Watermelon (97103) v1	cgywmB196
Cucsa.123720	Csa4G639750	Cucumber (Chinese Long) v2	cgycuB163
Cucsa.123720	MELO3C016747	Melon (DHL92) v3.5.1	cgymeB189
Cucsa.123720	ClCG05G019820	Watermelon (Charleston Gray)	cgywcgB194
Cucsa.123720	CSPI04G24020	Wild cucumber (PI 183967)	cgycpiB164
Cucsa.123720	CmaCh01G018460	Cucurbita maxima (Rimu)	cgycmaB0323
Cucsa.123720	CmoCh01G019040	Cucurbita moschata (Rifu)	cgycmoB0319
Cucsa.123720	CmoCh09G002150	Cucurbita moschata (Rifu)	cgycmoB0317
Cucsa.123720	Lsi04G015690	Bottle gourd (USVL1VR-Ls)	cgylsiB186
Cucsa.123720	Cp4.1LG02g07000	Cucurbita pepo (Zucchini)	cgycpeB0311
Cucsa.123720	Cp4.1LG06g01590	Cucurbita pepo (Zucchini)	cgycpeB0313
Cucsa.123720	MELO3C016747.2	Melon (DHL92) v3.6.1	cgymedB187
Cucsa.123720	Cla97C05G101440	Watermelon (97103) v2	cgywmbB190
Cucsa.123720	Bhi09G002737	Wax gourd	cgywgoB223
Cucsa.123720	Carg08774	Silver-seed gourd	carcgyB0123
Cucsa.123720	Carg10598	Silver-seed gourd	carcgyB0872

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.123720	Cucumber (Chinese Long) v3	cgycucB167
Cucsa.123720	Cucurbita maxima (Rimu)	cgycmaB0320