Cucsa.012810 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.012810
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	tRNA dimethylallyltransferase
Location	scaffold00164 : 566840 .. 568390 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTCCGAAGCAGAGGAGGAGCTCTCTTCTCCAGAGGACGAGTGTCTTAACAAGCTTCCAGAAAAGAAGAAGCCCTTGTATTCTCACAGTTTGCCTTGCGTTGAGGCTTGGTTGAAGAACTTAGGATTTTACCAAaGTAAAAaGGATTGAGCTGTGTGGCTGATCGAGAAGCCTGAATGGCACGCTCAGCTCTCACTCGATGTCACCGACCTCTATGTAAGGTTCGCCCCTTTTTTGTGATTTTGTGATAAGAATTTGTGTTTTTCAATTTGGGTTTTTTTtGTTTATCTTTTAATTGCATTTTCTGATCAGGGTTATTTCGTAAGTTGCAATGGGAATGAATTGTTCGCTGTTGTTTTGGTTTTAGAATTTTGTTAGAATTTTCTTTTTTCATGACTGCCTTTTGGGTTGTTTAACTTATGTAAGATATCAATGGAGTTGAATTCAGGCATTGGACTCTGGGGTTGTGTTCATTCAAGCAAGCATCTATATGTAACTGCCTCTATTAGGTTGTCTAAATGATGCTTGTTTTATGTATTAAATCTCCATGGAAAGAAGATAATTATGCCTTGCTGTTGTCTTtATTGAAACTATGGTCAAAACTGAGCTGGGATGATGGACAATCCTCTAGCTTTTATCCTCTGGACTGGTTATAGATTAATAATAAAGTGGTATATCCAGTTCAATTCATCCTTGTGGAGTAGAGGGTAGTGAAGATTCTTGGGTGAGAATGAAAAATAATGTCAAACAAGTAAGCTAAGAACGAACCACAATCCATAATTTGTTTTTtGTTACCATAAGATGGCAGAATAGAGCATGGAGAATTTTATAGCTTGGCTGATACCTTTtGTTTTTTTTTTTTTTTTtttACACTTCATTGAGTTTTAAATTCTCTTGGGTGCTAGTAAACGTCTTGAACTGCATCAAAATATTTAAAACAAGGAATAAACTACCCTAACTTTTTGGTAGTGAGAGTAGGAGAGAGACTAGCTGGTGTATTTCCTTCCAAGTTCCAATCATGGCGTTTTAGTGACTGACTCTGATACTTTGTTATCTATCAGTGTGTAAGAATACTTTCTTACTTACGATTTGATTATCAAACGCAAGTATTACAATATAAAACCAGATAACCTCTTCGCAGTCCTGCTAAAAAATTCAGGTAATGTCTACCAAACATAGAATCACTGTTCTGTCCATATTACAAAGCATAACGATAGCAAAGAGATTATGTACTAATTGTACTAATGAATTTTTCCCTGGTCGGTTTGACAGTTCCCTTATTGTTCTTTCTAATGAATAAATGATTAGGGGCTAACACATTGCCCCCAACACCTCGCTCACACACGAAAAGGAAAACAGGAACGAAAAAAAAAaGAACCCTTTCTATTATTGGTTGTGAGGTAAGTTCAGCCACTGGTTTTGAATTTGATACTCACACAATGCAGATATCTCAAGAGTGGACCAGGAAATCTTGAGAAAGATGTAGAGAGAAGATTTAGCTATGCTTTAAGCAGAGAAGATATTGAGAATGCTGTGCTTGGAGGACCTTGA

mRNA sequence

TCTTCCGAAGCAGAGGAGGAGCTCTCTTCTCCAGAGGACGAGTGTCTTAACAAGCTTCCAGAAAAGAAGAAGCCCTTGTATTCTCACAGTTTGCCTTGCGTTGAGGCTTGGTTGAAGAACTTAGGATTTTACCAAAAATACTTTCTTACTTACGATTTGATTATCAAACGCAAGTATTACAATATAAAACCAGATAACCTCTTCGCAGTCCTGCTAAAAAATTCAGGTAATAGTGGACCAGGAAATCTTGAGAAAGATGTAGAGAGAAGATTTAGCTATGCTTTAAGCAGAGAAGATATTGAGAATGCTGTGCTTGGAGGACCTTGA

Coding sequence (CDS)

TCTTCCGAAGCAGAGGAGGAGCTCTCTTCTCCAGAGGACGAGTGTCTTAACAAGCTTCCAGAAAAGAAGAAGCCCTTGTATTCTCACAGTTTGCCTTGCGTTGAGGCTTGGTTGAAGAACTTAGGATTTTACCAAaAATACTTTCTTACTTACGATTTGATTATCAAACGCAAGTATTACAATATAAAACCAGATAACCTCTTCGCAGTCCTGCTAAAAAATTCAGGTAATAGTGGACCAGGAAATCTTGAGAAAGATGTAGAGAGAAGATTTAGCTATGCTTTAAGCAGAGAAGATATTGAGAATGCTGTGCTTGGAGGACCTTGA

Protein sequence

SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQKYFLTYDLIIKRKYYNIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP*

Homology

[Close]

BLAST of Cucsa.012810 vs. TrEMBL
Match: M5X0W9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012623mg PE=4 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 6.3e-29
Identity = 73/111 (65.77%), Postives = 86/111 (77.48%), Query Frame = 1


Query: 2   SEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ-KYFLTYDLIIKRKY- 61
           SEAEEELS+PEDE L +LP+KKKPLYSHSLPC+EAWL+NLGFYQ K      L+ K ++ 
Sbjct: 55  SEAEEELSAPEDEWLKRLPDKKKPLYSHSLPCIEAWLRNLGFYQSKEDRAVWLVEKPEWQ 114

Query: 62  --YNIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
              ++   +L+   LK    +GPGNLEKDVERRFSYALSREDIENAVLGGP
Sbjct: 115 AQLSLDVTDLYVRYLK----TGPGNLEKDVERRFSYALSREDIENAVLGGP 161

[Close]

BLAST of Cucsa.012810 vs. TrEMBL
Match: B9RIN8_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1581360 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 4.1e-28
Identity = 74/112 (66.07%), Postives = 87/112 (77.68%), Query Frame = 1


Query: 1   SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ-KYFLTYDLIIKRKY 60
           SSEAEEELSS EDE L KLP+KKKPLYSHSLPC+EAWLK+LGF+Q K      LI K ++
Sbjct: 63  SSEAEEELSSTEDEWLKKLPDKKKPLYSHSLPCIEAWLKDLGFHQSKDDRAIWLIEKLEW 122

Query: 61  Y---NIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
           +   ++   +L+   LK    SGPG+LEKDVERRFSYALSREDIENA+LGGP
Sbjct: 123 HAQLSLDITDLYIRYLK----SGPGSLEKDVERRFSYALSREDIENAILGGP 170

[Close]

BLAST of Cucsa.012810 vs. TrEMBL
Match: W9RU92_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_011480 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 5.3e-28
Identity = 75/112 (66.96%), Postives = 86/112 (76.79%), Query Frame = 1


Query: 1   SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ-KYFLTYDLIIKRKY 60
           SSEAEE LSSPEDE L KLP+KKKPLYSHSLPC+EAWL++LGFYQ K      L+ K  +
Sbjct: 63  SSEAEE-LSSPEDEWLKKLPDKKKPLYSHSLPCIEAWLRSLGFYQSKEDRAVWLVEKADW 122

Query: 61  Y---NIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
           +   ++   +L+   LK    SGPGNLEKDVERRFSYALSREDIENAVLGGP
Sbjct: 123 HAQLSLDVTDLYIRYLK----SGPGNLEKDVERRFSYALSREDIENAVLGGP 169

[Close]

BLAST of Cucsa.012810 vs. TrEMBL
Match: A0A067JBP3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00036 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.5e-27
Identity = 70/112 (62.50%), Postives = 83/112 (74.11%), Query Frame = 1


Query: 1   SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQKYFLTYDLIIK---- 60
           SSEAEEELSS EDE L +LP+KKKPLYSHSLPC+EAWL++LGFYQ         ++    
Sbjct: 62  SSEAEEELSSTEDEWLKRLPDKKKPLYSHSLPCIEAWLRDLGFYQSKDDRAVWFVEKPEW 121

Query: 61  RKYYNIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
           R   ++   +L+   LK    SGPG+LEKDVERRFSYALSREDIENA+LGGP
Sbjct: 122 RAQLSLDMTDLYIRYLK----SGPGSLEKDVERRFSYALSREDIENAILGGP 169

[Close]

BLAST of Cucsa.012810 vs. TrEMBL
Match: A0A067EYZ7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g030903mg PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 1.7e-26
Identity = 69/112 (61.61%), Postives = 82/112 (73.21%), Query Frame = 1


Query: 1   SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQKY-FLTYDLIIKRKY 60
           S EAEEEL S EDE L KLPEK KPLYSHSLPC+EAWL++LGF+Q        L+ K  +
Sbjct: 62  SFEAEEELPSTEDEWLEKLPEKNKPLYSHSLPCIEAWLRSLGFHQSSDDRAVWLVEKHDW 121

Query: 61  Y---NIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
           +   ++   +L+   LKN    GPGNLEKD+ERRFSYALSREDIENA+LGGP
Sbjct: 122 HAQLSLDMTDLYIRYLKN----GPGNLEKDIERRFSYALSREDIENAILGGP 169

[Close]

BLAST of Cucsa.012810 vs. TAIR10
Match: AT5G52960.1 (AT5G52960.1 unknown protein)

HSP 1 Score: 118.2 bits (295), Expect = 3.1e-27
Identity = 63/108 (58.33%), Postives = 76/108 (70.37%), Query Frame = 1


Query: 1   SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQKYFLTYDLIIKRKYY 60
           SS+AEE +S  EDE L KLPEK KPLYSHSLPC+EAWL+ LGFYQ        +I++  +
Sbjct: 64  SSDAEE-VSDTEDEWLKKLPEKNKPLYSHSLPCIEAWLRKLGFYQSKDDRAVWLIQKPDW 123

Query: 61  NIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
           + +       L      SGPGNLE+D+ERRFSYALSRED ENA+LGGP
Sbjct: 124 HAQLSLDVTDLCIRYMKSGPGNLERDMERRFSYALSREDTENAILGGP 170

[Close]

BLAST of Cucsa.012810 vs. NCBI nr
Match: gi|778680932|ref|XP_011651422.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101209888, partial [Cucumis sativus])

HSP 1 Score: 151.0 bits (380), Expect = 1.2e-33
Identity = 83/112 (74.11%), Postives = 90/112 (80.36%), Query Frame = 1


Query: 1   SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ-KYFLTYDLIIKRKY 60
           SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ K      LI K ++
Sbjct: 60  SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQSKKDXAVWLIEKPEW 119

Query: 61  Y---NIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
           +   ++   +L+   LK    SGPGNLEKDVERRFSYALSREDIENAVLGGP
Sbjct: 120 HAQLSLDVTDLYVRYLK----SGPGNLEKDVERRFSYALSREDIENAVLGGP 167

[Close]

BLAST of Cucsa.012810 vs. NCBI nr
Match: gi|659077667|ref|XP_008439322.1| (PREDICTED: uncharacterized protein LOC103484146 [Cucumis melo])

HSP 1 Score: 139.4 bits (350), Expect = 3.7e-30
Identity = 81/112 (72.32%), Postives = 88/112 (78.57%), Query Frame = 1


Query: 1   SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ-KYFLTYDLIIKRKY 60
           SSEAEE LSSPEDE LNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ K      LI K ++
Sbjct: 58  SSEAEE-LSSPEDEWLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQSKEDRAMWLIEKPEW 117

Query: 61  Y---NIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
           +   ++   +L+   LK    SGPGNLEKDVERRFSYALSREDIENAVLGGP
Sbjct: 118 HAQLSLDVTDLYIRYLK----SGPGNLEKDVERRFSYALSREDIENAVLGGP 164

[Close]

BLAST of Cucsa.012810 vs. NCBI nr
Match: gi|645258254|ref|XP_008234801.1| (PREDICTED: uncharacterized protein LOC103333685 [Prunus mume])

HSP 1 Score: 134.8 bits (338), Expect = 9.0e-29
Identity = 73/111 (65.77%), Postives = 86/111 (77.48%), Query Frame = 1


Query: 2   SEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ-KYFLTYDLIIKRKY- 61
           SEAEEELS+PEDE L +LP+KKKPLYSHSLPC+EAWL+NLGFYQ K      L+ K ++ 
Sbjct: 55  SEAEEELSAPEDEWLKRLPDKKKPLYSHSLPCIEAWLRNLGFYQSKEDRAVWLVEKPEWQ 114

Query: 62  --YNIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
              ++   +L+   LK    +GPGNLEKDVERRFSYALSREDIENAVLGGP
Sbjct: 115 AQLSLDVTDLYVRYLK----TGPGNLEKDVERRFSYALSREDIENAVLGGP 161

[Close]

BLAST of Cucsa.012810 vs. NCBI nr
Match: gi|596009460|ref|XP_007218528.1| (hypothetical protein PRUPE_ppa012623mg [Prunus persica])

HSP 1 Score: 134.8 bits (338), Expect = 9.0e-29
Identity = 73/111 (65.77%), Postives = 86/111 (77.48%), Query Frame = 1


Query: 2   SEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ-KYFLTYDLIIKRKY- 61
           SEAEEELS+PEDE L +LP+KKKPLYSHSLPC+EAWL+NLGFYQ K      L+ K ++ 
Sbjct: 55  SEAEEELSAPEDEWLKRLPDKKKPLYSHSLPCIEAWLRNLGFYQSKEDRAVWLVEKPEWQ 114

Query: 62  --YNIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
              ++   +L+   LK    +GPGNLEKDVERRFSYALSREDIENAVLGGP
Sbjct: 115 AQLSLDVTDLYVRYLK----TGPGNLEKDVERRFSYALSREDIENAVLGGP 161

[Close]

BLAST of Cucsa.012810 vs. NCBI nr
Match: gi|255545092|ref|XP_002513607.1| (PREDICTED: uncharacterized protein LOC8277054 [Ricinus communis])

HSP 1 Score: 132.1 bits (331), Expect = 5.8e-28
Identity = 74/112 (66.07%), Postives = 87/112 (77.68%), Query Frame = 1


Query: 1   SSEAEEELSSPEDECLNKLPEKKKPLYSHSLPCVEAWLKNLGFYQ-KYFLTYDLIIKRKY 60
           SSEAEEELSS EDE L KLP+KKKPLYSHSLPC+EAWLK+LGF+Q K      LI K ++
Sbjct: 63  SSEAEEELSSTEDEWLKKLPDKKKPLYSHSLPCIEAWLKDLGFHQSKDDRAIWLIEKLEW 122

Query: 61  Y---NIKPDNLFAVLLKNSGNSGPGNLEKDVERRFSYALSREDIENAVLGGP 109
           +   ++   +L+   LK    SGPG+LEKDVERRFSYALSREDIENA+LGGP
Sbjct: 123 HAQLSLDITDLYIRYLK----SGPGSLEKDVERRFSYALSREDIENAILGGP 170

The following BLAST results are available for this feature:

BLAST of Cucsa.012810 vs. TrEMBL


Match Name	E-value	Identity	Description
M5X0W9_PRUPE	6.3e-29	65.77	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012623mg PE=4 SV=1	[more]
B9RIN8_RICCO	4.1e-28	66.07	Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1581360 PE=4 SV=1	[more]
W9RU92_9ROSA	5.3e-28	66.96	Uncharacterized protein OS=Morus notabilis GN=L484_011480 PE=4 SV=1	[more]
A0A067JBP3_JATCU	1.5e-27	62.50	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00036 PE=4 SV=1	[more]
A0A067EYZ7_CITSI	1.7e-26	61.61	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g030903mg PE=4 SV=1	[more]

BLAST of Cucsa.012810 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G52960.1	3.1e-27	58.33	unknown protein	[more]

BLAST of Cucsa.012810 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778680932\|ref\|XP_011651422.1\|	1.2e-33	74.11	PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101209888, partial [C...	[more]
gi\|659077667\|ref\|XP_008439322.1\|	3.7e-30	72.32	PREDICTED: uncharacterized protein LOC103484146 [Cucumis melo]	[more]
gi\|645258254\|ref\|XP_008234801.1\|	9.0e-29	65.77	PREDICTED: uncharacterized protein LOC103333685 [Prunus mume]	[more]
gi\|596009460\|ref\|XP_007218528.1\|	9.0e-29	65.77	hypothetical protein PRUPE_ppa012623mg [Prunus persica]	[more]
gi\|255545092\|ref\|XP_002513607.1\|	5.8e-28	66.07	PREDICTED: uncharacterized protein LOC8277054 [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR021489	DUF3143

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.012810.1	Cucsa.012810.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021489	Protein of unknown function DUF3143	PFAM	PF11341	DUF3143	coord: 81..107 score: 1.
None	No IPR available	PANTHER	PTHR35765	FAMILY NOT NAMED	coord: 1..108 score: 4.0
None	No IPR available	PANTHER	PTHR35765:SF2	SUBFAMILY NOT NAMED	coord: 1..108 score: 4.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.012810	Cla004219	Watermelon (97103) v1	cgywmB366
Cucsa.012810	MELO3C006968	Melon (DHL92) v3.5.1	cgymeB342
Cucsa.012810	ClCG05G008590	Watermelon (Charleston Gray)	cgywcgB344
Cucsa.012810	CmaCh16G000840	Cucurbita maxima (Rimu)	cgycmaB0549
Cucsa.012810	CmoCh16G000870	Cucurbita moschata (Rifu)	cgycmoB0550
Cucsa.012810	Lsi05G011700	Bottle gourd (USVL1VR-Ls)	cgylsiB323
Cucsa.012810	Cp4.1LG14g06340	Cucurbita pepo (Zucchini)	cgycpeB0530
Cucsa.012810	MELO3C006968.2	Melon (DHL92) v3.6.1	cgymedB337
Cucsa.012810	Cla97C05G090740	Watermelon (97103) v2	cgywmbB350
Cucsa.012810	Bhi01G001058	Wax gourd	cgywgoB392
Cucsa.012810	Carg15092	Silver-seed gourd	carcgyB1106

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.012810	Cucurbita maxima (Rimu)	cgycmaB0550
Cucsa.012810	Cucurbita moschata (Rifu)	cgycmoB0551
Cucsa.012810	Wild cucumber (PI 183967)	cgycpiB299
Cucsa.012810	Wild cucumber (PI 183967)	cgycpiB300
Cucsa.012810	Cucumber (Chinese Long) v2	cgycuB290
Cucsa.012810	Cucumber (Chinese Long) v2	cgycuB291
Cucsa.012810	Cucurbita pepo (Zucchini)	cgycpeB0529
Cucsa.012810	Bottle gourd (USVL1VR-Ls)	cgylsiB321
Cucsa.012810	Silver-seed gourd	carcgyB0616
Cucsa.012810	Cucumber (Chinese Long) v3	cgycucB303